SEC15B cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	SEC15B
Entrez Gene ID	828060
Full Name	exocyst complex component sec15B
Synonyms	T14P8.16,T14P8_16,exocyst complex component sec15B
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001340371.1	NP_001319844.1	exocyst complex component sec15B
NM_116468.4	NP_567229.2	exocyst complex component sec15B

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os01g0351300	NP_001042985.1
Arabidopsis thaliana (thale cress)	SEC15B	NP_567229.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SEC15b interacts with SEC10, and functions as a subunit in a exocyst complex that plays important roles in morphogenesis.
Title: An exocyst complex functions in plant cell growth in Arabidopsis and tobacco.

The following SEC15B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEC15B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb15717	NM_001340371.1 Latest version!	Arabidopsis thaliana exocyst complex component sec15B (SEC15B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb15717	NM_116468.4 Latest version!	Arabidopsis thaliana exocyst complex component sec15B (SEC15B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEC15B ( NM_001340371.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001319844.1 Arabidopsis thaliana exocyst complex component sec15B (SEC15B), mRNA.

Price & Availability↑

CloneID	OAb15717
Clone ID Related Accession (Same CDS sequence)	NM_001340371.1 , NM_116468.4
Accession Version	NM_001340371.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	exocyst complex component sec15B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGCAATCGT CGAAAGGACG GCGGAAAGTT GGCTCCACAA CCGCCGGAGC CGGTATAGAT 
TCGGCGGAGA AGCTCGACGA GCTCTTGATA TCGTCTGCCA TTTGTAACGG TGAAGATTTA 
GGACCATTTG TTCGGAAAAC TTTCGGTACA GGGAAACCAG AGACTCTTCT TCACCATCTC 
AAGTTTTTCG CACGATCTAA GGAATCAGAG ATCGAAGAGG TATGTAAAGC TCACTATCAA 
GACTTTATCC ACGCCGTAGA CGATCTTAAA TCGCTTCTCT CAGATGTTGA ATCGCTTAAA 
TCTGCGCTTT CCGATTCAAA CTCTAAACTC CAATCTGTTG CGGCGCCGCT TCTTTCGTCT 
CTCGATTCGC TTGTTGAGGC TCAGACTGTG TCTAAGAACG TCGATCTGGC GATTGGAGCG 
GTGACTCATT GTGTTCGTGT TATGGAACTT GTCTCTCGAG CTAATCAGCA TCTTCAGAGC 
GGTAATTTCT ACATGGCGTT GAAATGTGTG GACTCGATTG AGAGTGATTT CATGGAGAAA 
ACTCCTTCGT CAACGCTGAA ACGGATGCTT GAGAATAGGA TTCCGGCGAT TCGATCTTAT 
GTGGAGAGGA AAGTGAATAA GGAGTTTGGA GATTGGTTGG TTGAGATCCG TGTGGTGAGT 
CGGAATTTAG GTCAGTTGGC TATTGGTGAA GCTTCCGCGG CTCGTCAGCG AGAGGAGGAG 
CTCAGGATCA AGCAGAGACA AGCGGAGGAG CAGAGTAGAC TTAGTCTACG CGATTGCGTT 
TATGCGCTAA ACGAAGAAGA GGATGATGAA TTTGGTTCTG GCCATGAAGG CAGCGATGGT 
GGTAGTTCCG GAGGTGGATT GCTAGGTTTT GATTTGACAC CACTTTATAG AGCTTATCAC 
ATTCATCAGA CACTATCTCT TGGGGATACT TTTAAGCAGT ACTATTATAA CAATAGGGAT 
CTTCAGCTTA CCTCTGACTT CCAGATTGCT GGGTTTTTCA TAGTAGAGGA TAGAGTTTTA 
AGGACTGGAG GAGGGTTGAT TTCGAAGCTG GAAGTGGAGA CTTTGTGGGA TACTGCTGTT 
ACTAAGATGT GTGCTGTGTT GGAGGATCAG TTCTCTAGAA TGCAGACGGC TAATCATCTC 
TTACTGATTA AGGATTATGT GAGCTTGTTG GGGGTGAGTC TACGCAGGTA TGGCTATGCT 
GTTGATTCCC TTCTCGAAGT ATTAAGCAAG CACAGGGACA AGTATCATGA GTTGTTACTA 
TCTGATTGCC GTAAACAGAT CACAGAAGCT TTATCAGCAG ATAAGTTTGA GCAGATGTTG 
ATGAAGAAAG AATATGAGTA TTCCATGAAT GTGCTCTCTT TCCAGTTACA AACATCAGAA 
ATAGTACCGG CATTCCCTTT CATTGCTCCA TTTTCGACCA CCGTCCCAGA TTGTTGCCGG 
ATTGTGAGGT CTTTTATCGA GGATTCGGTT AGTTTCATGT CTCACGGAGG TCAGCTTGAT 
TTCTATGATG TAGTGAAGAA GTATCTGGAT AGGCTTCTAG GCGAGGTTCT CGATGAGGCT 
CTGTTGAAGC TGATTAGCAC GTCGGTTCAT GGAGTTTCTC AGGCAATGCA AGTTGCAGCA 
AACATGGCCG TATTTGAACG AGCTTGCGAT TTCTTCTTCC GTCACGCTGC ACATCTTTCA 
GGAGTTCCTT TGAGAATGGC GGAGAGAGGT AGGAGGCATT TCCCATTGAC GAAATCTCAA 
AACACTGCAG AAGATACACT CTCAGGAATG CTTAAGAAGA AGATAGATGG TTTCATGACA 
TTACTCGAGA ATGTCAATTG GACAAGCGAC GATATACCAC AAGGTGGGAA TGAGTACATG 
AACGAAGTGT TGATCTACTT GGAAACTTTG GTTTCCACCG CACAACAGAT CTTACCCGCT 
AAAGTCTTGA AAAGGGTTTT GCGTGATGTC CTTGCTCACA TTTCTGAGAA AATCGTTGGA 
ACGTTATGCG GGGATCTAGT AAAAAGATTG AGCATGGCTG CAATCAAAGG ACTTGATGTG 
GATATTCAGT TGTTGGACTC GTTTACAGAG AACCTAACTC CATTGCTCAC GGATAAAGAA 
GCAAGAGAGA TGAAAAAAGC GTTTGTCGAG ATAAGACAGA TGATCAATTT ACTTCTGAGT 
AGTCATCCTG AGAATTTTGT AAACCCGGTG ATCAGAGAAA GAAGCTACAA TGCTTTGGAT 
TACCGGAAAG TAGCCACGGT TTCAGAGAAA TTCAGAGATC CATCAGATAG TATTTTCGGG 
ACTTTTGGAA CAAGAGGGTC GAGGCAAAAC CCGAAGAACA AATCTTTAGA TGCGTTGATA 
AAGAGATTGA AAGATGTGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001319844.1
CDS	44..2407
Translation	MQSSKGRRKVGSTTAGAGIDSAEKLDELLISSAICNGEDLGPFVRKTFGTGKPETLLHHLKFFARSKESEIEEVCKAHYQDFIHAVDDLKSLLSDVESLKSALSDSNSKLQSVAAPLLSSLDSLVEAQTVSKNVDLAIGAVTHCVRVMELVSRANQHLQSGNFYMALKCVDSIESDFMEKTPSSTLKRMLENRIPAIRSYVERKVNKEFGDWLVEIRVVSRNLGQLAIGEASAARQREEELRIKQRQAEEQSRLSLRDCVYALNEEEDDEFGSGHEGSDGGSSGGGLLGFDLTPLYRAYHIHQTLSLGDTFKQYYYNNRDLQLTSDFQIAGFFIVEDRVLRTGGGLISKLEVETLWDTAVTKMCAVLEDQFSRMQTANHLLLIKDYVSLLGVSLRRYGYAVDSLLEVLSKHRDKYHELLLSDCRKQITEALSADKFEQMLMKKEYEYSMNVLSFQLQTSEIVPAFPFIAPFSTTVPDCCRIVRSFIEDSVSFMSHGGQLDFYDVVKKYLDRLLGEVLDEALLKLISTSVHGVSQAMQVAANMAVFERACDFFFRHAAHLSGVPLRMAERGRRHFPLTKSQNTAEDTLSGMLKKKIDGFMTLLENVNWTSDDIPQGGNEYMNEVLIYLETLVSTAQQILPAKVLKRVLRDVLAHISEKIVGTLCGDLVKRLSMAAIKGLDVDIQLLDSFTENLTPLLTDKEAREMKKAFVEIRQMINLLLSSHPENFVNPVIRERSYNALDYRKVATVSEKFRDPSDSIFGTFGTRGSRQNPKNKSLDALIKRLKDVS

Target ORF information:

RefSeq Version	NM_001340371.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana exocyst complex component sec15B (SEC15B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001340371.1

ORF Insert Sequence:

ATGCAATCGT CGAAAGGACG GCGGAAAGTT GGCTCCACAA CCGCCGGAGC CGGTATAGAT 
TCGGCGGAGA AGCTCGACGA GCTCTTGATA TCGTCTGCCA TTTGTAACGG TGAAGATTTA 
GGACCATTTG TTCGGAAAAC TTTCGGTACA GGGAAACCAG AGACTCTTCT TCACCATCTC 
AAGTTTTTCG CACGATCTAA GGAATCAGAG ATCGAAGAGG TATGTAAAGC TCACTATCAA 
GACTTTATCC ACGCCGTAGA CGATCTTAAA TCGCTTCTCT CAGATGTTGA ATCGCTTAAA 
TCTGCGCTTT CCGATTCAAA CTCTAAACTC CAATCTGTTG CGGCGCCGCT TCTTTCGTCT 
CTCGATTCGC TTGTTGAGGC TCAGACTGTG TCTAAGAACG TCGATCTGGC GATTGGAGCG 
GTGACTCATT GTGTTCGTGT TATGGAACTT GTCTCTCGAG CTAATCAGCA TCTTCAGAGC 
GGTAATTTCT ACATGGCGTT GAAATGTGTG GACTCGATTG AGAGTGATTT CATGGAGAAA 
ACTCCTTCGT CAACGCTGAA ACGGATGCTT GAGAATAGGA TTCCGGCGAT TCGATCTTAT 
GTGGAGAGGA AAGTGAATAA GGAGTTTGGA GATTGGTTGG TTGAGATCCG TGTGGTGAGT 
CGGAATTTAG GTCAGTTGGC TATTGGTGAA GCTTCCGCGG CTCGTCAGCG AGAGGAGGAG 
CTCAGGATCA AGCAGAGACA AGCGGAGGAG CAGAGTAGAC TTAGTCTACG CGATTGCGTT 
TATGCGCTAA ACGAAGAAGA GGATGATGAA TTTGGTTCTG GCCATGAAGG CAGCGATGGT 
GGTAGTTCCG GAGGTGGATT GCTAGGTTTT GATTTGACAC CACTTTATAG AGCTTATCAC 
ATTCATCAGA CACTATCTCT TGGGGATACT TTTAAGCAGT ACTATTATAA CAATAGGGAT 
CTTCAGCTTA CCTCTGACTT CCAGATTGCT GGGTTTTTCA TAGTAGAGGA TAGAGTTTTA 
AGGACTGGAG GAGGGTTGAT TTCGAAGCTG GAAGTGGAGA CTTTGTGGGA TACTGCTGTT 
ACTAAGATGT GTGCTGTGTT GGAGGATCAG TTCTCTAGAA TGCAGACGGC TAATCATCTC 
TTACTGATTA AGGATTATGT GAGCTTGTTG GGGGTGAGTC TACGCAGGTA TGGCTATGCT 
GTTGATTCCC TTCTCGAAGT ATTAAGCAAG CACAGGGACA AGTATCATGA GTTGTTACTA 
TCTGATTGCC GTAAACAGAT CACAGAAGCT TTATCAGCAG ATAAGTTTGA GCAGATGTTG 
ATGAAGAAAG AATATGAGTA TTCCATGAAT GTGCTCTCTT TCCAGTTACA AACATCAGAA 
ATAGTACCGG CATTCCCTTT CATTGCTCCA TTTTCGACCA CCGTCCCAGA TTGTTGCCGG 
ATTGTGAGGT CTTTTATCGA GGATTCGGTT AGTTTCATGT CTCACGGAGG TCAGCTTGAT 
TTCTATGATG TAGTGAAGAA GTATCTGGAT AGGCTTCTAG GCGAGGTTCT CGATGAGGCT 
CTGTTGAAGC TGATTAGCAC GTCGGTTCAT GGAGTTTCTC AGGCAATGCA AGTTGCAGCA 
AACATGGCCG TATTTGAACG AGCTTGCGAT TTCTTCTTCC GTCACGCTGC ACATCTTTCA 
GGAGTTCCTT TGAGAATGGC GGAGAGAGGT AGGAGGCATT TCCCATTGAC GAAATCTCAA 
AACACTGCAG AAGATACACT CTCAGGAATG CTTAAGAAGA AGATAGATGG TTTCATGACA 
TTACTCGAGA ATGTCAATTG GACAAGCGAC GATATACCAC AAGGTGGGAA TGAGTACATG 
AACGAAGTGT TGATCTACTT GGAAACTTTG GTTTCCACCG CACAACAGAT CTTACCCGCT 
AAAGTCTTGA AAAGGGTTTT GCGTGATGTC CTTGCTCACA TTTCTGAGAA AATCGTTGGA 
ACGTTATGCG GGGATCTAGT AAAAAGATTG AGCATGGCTG CAATCAAAGG ACTTGATGTG 
GATATTCAGT TGTTGGACTC GTTTACAGAG AACCTAACTC CATTGCTCAC GGATAAAGAA 
GCAAGAGAGA TGAAAAAAGC GTTTGTCGAG ATAAGACAGA TGATCAATTT ACTTCTGAGT 
AGTCATCCTG AGAATTTTGT AAACCCGGTG ATCAGAGAAA GAAGCTACAA TGCTTTGGAT 
TACCGGAAAG TAGCCACGGT TTCAGAGAAA TTCAGAGATC CATCAGATAG TATTTTCGGG 
ACTTTTGGAA CAAGAGGGTC GAGGCAAAAC CCGAAGAACA AATCTTTAGA TGCGTTGATA 
AAGAGATTGA AAGATGTGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEC15B ( NM_116468.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_567229.2 Arabidopsis thaliana exocyst complex component sec15B (SEC15B), mRNA.

Price & Availability↑

CloneID	OAb15717
Clone ID Related Accession (Same CDS sequence)	NM_001340371.1 , NM_116468.4
Accession Version	NM_116468.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	exocyst complex component sec15B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On May 26, 2011 this sequence version replaced NM_116468.3.

ATGCAATCGT CGAAAGGACG GCGGAAAGTT GGCTCCACAA CCGCCGGAGC CGGTATAGAT 
TCGGCGGAGA AGCTCGACGA GCTCTTGATA TCGTCTGCCA TTTGTAACGG TGAAGATTTA 
GGACCATTTG TTCGGAAAAC TTTCGGTACA GGGAAACCAG AGACTCTTCT TCACCATCTC 
AAGTTTTTCG CACGATCTAA GGAATCAGAG ATCGAAGAGG TATGTAAAGC TCACTATCAA 
GACTTTATCC ACGCCGTAGA CGATCTTAAA TCGCTTCTCT CAGATGTTGA ATCGCTTAAA 
TCTGCGCTTT CCGATTCAAA CTCTAAACTC CAATCTGTTG CGGCGCCGCT TCTTTCGTCT 
CTCGATTCGC TTGTTGAGGC TCAGACTGTG TCTAAGAACG TCGATCTGGC GATTGGAGCG 
GTGACTCATT GTGTTCGTGT TATGGAACTT GTCTCTCGAG CTAATCAGCA TCTTCAGAGC 
GGTAATTTCT ACATGGCGTT GAAATGTGTG GACTCGATTG AGAGTGATTT CATGGAGAAA 
ACTCCTTCGT CAACGCTGAA ACGGATGCTT GAGAATAGGA TTCCGGCGAT TCGATCTTAT 
GTGGAGAGGA AAGTGAATAA GGAGTTTGGA GATTGGTTGG TTGAGATCCG TGTGGTGAGT 
CGGAATTTAG GTCAGTTGGC TATTGGTGAA GCTTCCGCGG CTCGTCAGCG AGAGGAGGAG 
CTCAGGATCA AGCAGAGACA AGCGGAGGAG CAGAGTAGAC TTAGTCTACG CGATTGCGTT 
TATGCGCTAA ACGAAGAAGA GGATGATGAA TTTGGTTCTG GCCATGAAGG CAGCGATGGT 
GGTAGTTCCG GAGGTGGATT GCTAGGTTTT GATTTGACAC CACTTTATAG AGCTTATCAC 
ATTCATCAGA CACTATCTCT TGGGGATACT TTTAAGCAGT ACTATTATAA CAATAGGGAT 
CTTCAGCTTA CCTCTGACTT CCAGATTGCT GGGTTTTTCA TAGTAGAGGA TAGAGTTTTA 
AGGACTGGAG GAGGGTTGAT TTCGAAGCTG GAAGTGGAGA CTTTGTGGGA TACTGCTGTT 
ACTAAGATGT GTGCTGTGTT GGAGGATCAG TTCTCTAGAA TGCAGACGGC TAATCATCTC 
TTACTGATTA AGGATTATGT GAGCTTGTTG GGGGTGAGTC TACGCAGGTA TGGCTATGCT 
GTTGATTCCC TTCTCGAAGT ATTAAGCAAG CACAGGGACA AGTATCATGA GTTGTTACTA 
TCTGATTGCC GTAAACAGAT CACAGAAGCT TTATCAGCAG ATAAGTTTGA GCAGATGTTG 
ATGAAGAAAG AATATGAGTA TTCCATGAAT GTGCTCTCTT TCCAGTTACA AACATCAGAA 
ATAGTACCGG CATTCCCTTT CATTGCTCCA TTTTCGACCA CCGTCCCAGA TTGTTGCCGG 
ATTGTGAGGT CTTTTATCGA GGATTCGGTT AGTTTCATGT CTCACGGAGG TCAGCTTGAT 
TTCTATGATG TAGTGAAGAA GTATCTGGAT AGGCTTCTAG GCGAGGTTCT CGATGAGGCT 
CTGTTGAAGC TGATTAGCAC GTCGGTTCAT GGAGTTTCTC AGGCAATGCA AGTTGCAGCA 
AACATGGCCG TATTTGAACG AGCTTGCGAT TTCTTCTTCC GTCACGCTGC ACATCTTTCA 
GGAGTTCCTT TGAGAATGGC GGAGAGAGGT AGGAGGCATT TCCCATTGAC GAAATCTCAA 
AACACTGCAG AAGATACACT CTCAGGAATG CTTAAGAAGA AGATAGATGG TTTCATGACA 
TTACTCGAGA ATGTCAATTG GACAAGCGAC GATATACCAC AAGGTGGGAA TGAGTACATG 
AACGAAGTGT TGATCTACTT GGAAACTTTG GTTTCCACCG CACAACAGAT CTTACCCGCT 
AAAGTCTTGA AAAGGGTTTT GCGTGATGTC CTTGCTCACA TTTCTGAGAA AATCGTTGGA 
ACGTTATGCG GGGATCTAGT AAAAAGATTG AGCATGGCTG CAATCAAAGG ACTTGATGTG 
GATATTCAGT TGTTGGACTC GTTTACAGAG AACCTAACTC CATTGCTCAC GGATAAAGAA 
GCAAGAGAGA TGAAAAAAGC GTTTGTCGAG ATAAGACAGA TGATCAATTT ACTTCTGAGT 
AGTCATCCTG AGAATTTTGT AAACCCGGTG ATCAGAGAAA GAAGCTACAA TGCTTTGGAT 
TACCGGAAAG TAGCCACGGT TTCAGAGAAA TTCAGAGATC CATCAGATAG TATTTTCGGG 
ACTTTTGGAA CAAGAGGGTC GAGGCAAAAC CCGAAGAACA AATCTTTAGA TGCGTTGATA 
AAGAGATTGA AAGATGTGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_567229.2
CDS	44..2407
Translation	MQSSKGRRKVGSTTAGAGIDSAEKLDELLISSAICNGEDLGPFVRKTFGTGKPETLLHHLKFFARSKESEIEEVCKAHYQDFIHAVDDLKSLLSDVESLKSALSDSNSKLQSVAAPLLSSLDSLVEAQTVSKNVDLAIGAVTHCVRVMELVSRANQHLQSGNFYMALKCVDSIESDFMEKTPSSTLKRMLENRIPAIRSYVERKVNKEFGDWLVEIRVVSRNLGQLAIGEASAARQREEELRIKQRQAEEQSRLSLRDCVYALNEEEDDEFGSGHEGSDGGSSGGGLLGFDLTPLYRAYHIHQTLSLGDTFKQYYYNNRDLQLTSDFQIAGFFIVEDRVLRTGGGLISKLEVETLWDTAVTKMCAVLEDQFSRMQTANHLLLIKDYVSLLGVSLRRYGYAVDSLLEVLSKHRDKYHELLLSDCRKQITEALSADKFEQMLMKKEYEYSMNVLSFQLQTSEIVPAFPFIAPFSTTVPDCCRIVRSFIEDSVSFMSHGGQLDFYDVVKKYLDRLLGEVLDEALLKLISTSVHGVSQAMQVAANMAVFERACDFFFRHAAHLSGVPLRMAERGRRHFPLTKSQNTAEDTLSGMLKKKIDGFMTLLENVNWTSDDIPQGGNEYMNEVLIYLETLVSTAQQILPAKVLKRVLRDVLAHISEKIVGTLCGDLVKRLSMAAIKGLDVDIQLLDSFTENLTPLLTDKEAREMKKAFVEIRQMINLLLSSHPENFVNPVIRERSYNALDYRKVATVSEKFRDPSDSIFGTFGTRGSRQNPKNKSLDALIKRLKDVS

Target ORF information:

RefSeq Version	NM_116468.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana exocyst complex component sec15B (SEC15B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_116468.4

ORF Insert Sequence:

ATGCAATCGT CGAAAGGACG GCGGAAAGTT GGCTCCACAA CCGCCGGAGC CGGTATAGAT 
TCGGCGGAGA AGCTCGACGA GCTCTTGATA TCGTCTGCCA TTTGTAACGG TGAAGATTTA 
GGACCATTTG TTCGGAAAAC TTTCGGTACA GGGAAACCAG AGACTCTTCT TCACCATCTC 
AAGTTTTTCG CACGATCTAA GGAATCAGAG ATCGAAGAGG TATGTAAAGC TCACTATCAA 
GACTTTATCC ACGCCGTAGA CGATCTTAAA TCGCTTCTCT CAGATGTTGA ATCGCTTAAA 
TCTGCGCTTT CCGATTCAAA CTCTAAACTC CAATCTGTTG CGGCGCCGCT TCTTTCGTCT 
CTCGATTCGC TTGTTGAGGC TCAGACTGTG TCTAAGAACG TCGATCTGGC GATTGGAGCG 
GTGACTCATT GTGTTCGTGT TATGGAACTT GTCTCTCGAG CTAATCAGCA TCTTCAGAGC 
GGTAATTTCT ACATGGCGTT GAAATGTGTG GACTCGATTG AGAGTGATTT CATGGAGAAA 
ACTCCTTCGT CAACGCTGAA ACGGATGCTT GAGAATAGGA TTCCGGCGAT TCGATCTTAT 
GTGGAGAGGA AAGTGAATAA GGAGTTTGGA GATTGGTTGG TTGAGATCCG TGTGGTGAGT 
CGGAATTTAG GTCAGTTGGC TATTGGTGAA GCTTCCGCGG CTCGTCAGCG AGAGGAGGAG 
CTCAGGATCA AGCAGAGACA AGCGGAGGAG CAGAGTAGAC TTAGTCTACG CGATTGCGTT 
TATGCGCTAA ACGAAGAAGA GGATGATGAA TTTGGTTCTG GCCATGAAGG CAGCGATGGT 
GGTAGTTCCG GAGGTGGATT GCTAGGTTTT GATTTGACAC CACTTTATAG AGCTTATCAC 
ATTCATCAGA CACTATCTCT TGGGGATACT TTTAAGCAGT ACTATTATAA CAATAGGGAT 
CTTCAGCTTA CCTCTGACTT CCAGATTGCT GGGTTTTTCA TAGTAGAGGA TAGAGTTTTA 
AGGACTGGAG GAGGGTTGAT TTCGAAGCTG GAAGTGGAGA CTTTGTGGGA TACTGCTGTT 
ACTAAGATGT GTGCTGTGTT GGAGGATCAG TTCTCTAGAA TGCAGACGGC TAATCATCTC 
TTACTGATTA AGGATTATGT GAGCTTGTTG GGGGTGAGTC TACGCAGGTA TGGCTATGCT 
GTTGATTCCC TTCTCGAAGT ATTAAGCAAG CACAGGGACA AGTATCATGA GTTGTTACTA 
TCTGATTGCC GTAAACAGAT CACAGAAGCT TTATCAGCAG ATAAGTTTGA GCAGATGTTG 
ATGAAGAAAG AATATGAGTA TTCCATGAAT GTGCTCTCTT TCCAGTTACA AACATCAGAA 
ATAGTACCGG CATTCCCTTT CATTGCTCCA TTTTCGACCA CCGTCCCAGA TTGTTGCCGG 
ATTGTGAGGT CTTTTATCGA GGATTCGGTT AGTTTCATGT CTCACGGAGG TCAGCTTGAT 
TTCTATGATG TAGTGAAGAA GTATCTGGAT AGGCTTCTAG GCGAGGTTCT CGATGAGGCT 
CTGTTGAAGC TGATTAGCAC GTCGGTTCAT GGAGTTTCTC AGGCAATGCA AGTTGCAGCA 
AACATGGCCG TATTTGAACG AGCTTGCGAT TTCTTCTTCC GTCACGCTGC ACATCTTTCA 
GGAGTTCCTT TGAGAATGGC GGAGAGAGGT AGGAGGCATT TCCCATTGAC GAAATCTCAA 
AACACTGCAG AAGATACACT CTCAGGAATG CTTAAGAAGA AGATAGATGG TTTCATGACA 
TTACTCGAGA ATGTCAATTG GACAAGCGAC GATATACCAC AAGGTGGGAA TGAGTACATG 
AACGAAGTGT TGATCTACTT GGAAACTTTG GTTTCCACCG CACAACAGAT CTTACCCGCT 
AAAGTCTTGA AAAGGGTTTT GCGTGATGTC CTTGCTCACA TTTCTGAGAA AATCGTTGGA 
ACGTTATGCG GGGATCTAGT AAAAAGATTG AGCATGGCTG CAATCAAAGG ACTTGATGTG 
GATATTCAGT TGTTGGACTC GTTTACAGAG AACCTAACTC CATTGCTCAC GGATAAAGAA 
GCAAGAGAGA TGAAAAAAGC GTTTGTCGAG ATAAGACAGA TGATCAATTT ACTTCTGAGT 
AGTCATCCTG AGAATTTTGT AAACCCGGTG ATCAGAGAAA GAAGCTACAA TGCTTTGGAT 
TACCGGAAAG TAGCCACGGT TTCAGAGAAA TTCAGAGATC CATCAGATAG TATTTTCGGG 
ACTTTTGGAA CAAGAGGGTC GAGGCAAAAC CCGAAGAACA AATCTTTAGA TGCGTTGATA 
AAGAGATTGA AAGATGTGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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SEC15B cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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