ECA2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	ECA2
Entrez Gene ID	827984
Full Name	ER-type Ca2+-ATPase 2
Synonyms	ARABIDOPSIS THALIANA ER-TYPE CA2+-ATPASE 2,ATECA2,A_TM018A10.4,A_TM018A10_4,ER-type Ca2+-ATPase 2,T18A10.20,T18A10_20
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_116317.4	NP_191999.1	ER-type Ca2+-ATPase 2
NM_001340276.1	NP_001329712.1	ER-type Ca2+-ATPase 2

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os03g0281600	NP_001049744.1
Arabidopsis thaliana (thale cress)	ECA2	NP_191999.1
Arabidopsis thaliana (thale cress)	ECA4	NP_172246.3
Arabidopsis thaliana (thale cress)	ECA1	NP_172259.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ECA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ECA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb15645	NM_116317.4 Latest version!	Arabidopsis thaliana ER-type Ca2+-ATPase 2 (ECA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OAb15645	NM_001340276.1 Latest version!	Arabidopsis thaliana ER-type Ca2+-ATPase 2 (ECA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ECA2 ( NM_116317.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_191999.1 Arabidopsis thaliana ER-type Ca2+-ATPase 2 (ECA2), mRNA.

Price & Availability↑

CloneID	OAb15645
Clone ID Related Accession (Same CDS sequence)	NM_116317.4 , NM_001340276.1
Accession Version	NM_116317.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	ER-type Ca2+-ATPase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_116317.3.

ATGGAGGAGG AGAAGTCGTT CTCGGCGTGG TCTTGGTCTG TTGAGCAATG TTTGAAAGAG 
TACAAGACAA GATTAGACAA GGGTTTAACT AGCGAAGATG TCCAAATCCG CCGTCAAAAG 
TACGGTTTTA ACGAGCTTGC TAAGGAGAAA GGCAAGCCTT TATGGCATCT TGTATTGGAA 
CAATTCGACG ATACGCTTGT GAAGATTCTT CTCGGTGCTG CGTTTATCTC TTTCGTTTTA 
GCCTTTTTAG GTGAGGAACA CGGCTCAGGT TCAGGGTTCG AGGCCTTTGT TGAGCCTTTT 
GTGATTGTTT TGATTCTGAT TTTAAACGCT GTGGTTGGTG TGTGGCAAGA GAGTAATGCT 
GAGAAAGCAC TTGAGGCTCT TAAAGAGATG CAATGTGAAT CTGCAAAGGT TTTGAGAGAT 
GGGAATGTGT TGCCTAATTT GCCTGCTAGA GAGCTTGTTC CAGGGGATAT TGTGGAGTTA 
AATGTAGGAG ATAAAGTCCC TGCTGATATG AGAGTTTCCG GTTTGAAAAC ATCCACTTTA 
AGAGTCGAGC AAAGCTCGTT AACCGGTGAA GCAATGCCGG TTTTGAAAGG AGCGAATCTT 
GTAGTCATGG ATGATTGTGA ACTGCAAGGG AAAGAAAATA TGGTTTTCGC TGGAACAACG 
GTTGTTAACG GAAGCTGCGT TTGCATTGTG ACAAGTATAG GTATGGATAC GGAGATCGGG 
AAAATCCAGA GACAGATTCA CGAGGCGTCG CTTGAAGAGA GCGAGACTCC GTTAAAGAAG 
AAGCTAGACG AGTTTGGGAG TAGATTGACT ACAGCTATTT GCATTGTCTG TGTTCTTGTG 
TGGATGATAA ACTACAAGAA CTTTGTTTCT TGGGATGTTG TGGATGGCTA TAAACCTGTG 
AATATCAAGT TTTCATTCGA GAAATGTACT TACTACTTCA AAATCGCTGT TGCTCTCGCA 
GTGGCAGCGA TACCCGAGGG TTTACCCGCT GTGATCACGA CATGTTTAGC TTTAGGGACG 
AGGAAAATGG CGCAGAAGAA CGCGATAGTG AGGAAGCTCC CGAGTGTAGA GACACTCGGT 
TGCACAACTG TGATCTGTTC AGATAAAACC GGGACTTTAA CAACAAACCA GATGTCTGCA 
ACCGAATTCT TCACATTAGG CGGTAAAACA ACGACTACTC GAGTGTTTTC AGTCAGTGGT 
ACGACTTATG ATCCTAAAGA TGGTGGAATT GTGGATTGGG GCTGCAACAA TATGGATGCT 
AACTTGCAAG CTGTTGCTGA GATATGTTCA ATTTGTAATG ATGCTGGAGT GTTCTATGAA 
GGGAAGTTGT TTAGAGCAAC AGGTTTGCCT ACAGAAGCAG CATTGAAAGT TCTTGTTGAG 
AAGATGGGTA TCCCGGAGAA GAAGAACAGT GAGAACATCG AGGAAGTTAC AAACTTTTCA 
GACAATGGTA GCTCAGTGAA GCTAGCTTGT TGCGATTGGT GGAACAAAAG ATCGAAAAAG 
GTAGCGACAT TAGAGTTTGA TCGCGTTCGT AAGTCCATGA GTGTTATTGT GAGCGAACCA 
AATGGACAAA ACCGGCTTCT CGTTAAGGGT GCTGCTGAGA GTATACTTGA GAGAAGTTCT 
TTTGCACAGC TTGCTGATGG ATCTCTTGTA GCTTTAGATG AATCTAGCAG AGAAGTAATC 
CTTAAGAAAC ATTCTGAAAT GACTTCAAAA GGATTAAGAT GTTTAGGATT AGCTTACAAA 
GACGAATTAG GAGAGTTTTC GGATTACTCT TCCGAAGAAC ATCCTTCACA CAAGAAGCTT 
TTGGACCCTT CTAGCTATTC GAACATCGAA ACAAATCTAA TCTTTGTTGG AGTTGTTGGT 
CTAAGAGATC CTCCGCGTGA AGAAGTTGGA AGAGCAATTG AAGATTGCAG AGACGCAGGG 
ATAAGAGTAA TGGTTATAAC TGGAGATAAC AAATCAACAG CTGAAGCTAT TTGTTGTGAA 
ATTAGATTGT TTTCGGAGAA TGAAGATCTT TCGCAGAGTA GCTTCACTGG TAAAGAGTTT 
ATGTCTCTTC CGGCTTCACG GAGATCCGAG ATTCTGTCAA AATCTGGAGG GAAAGTGTTC 
TCTCGAGCTG AGCCAAGGCA TAAGCAAGAA ATCGTTAGGA TGCTTAAGGA AATGGGAGAA 
ATAGTTGCCA TGACAGGTGA TGGTGTGAAT GATGCTCCTG CTTTGAAACT TGCTGACATT 
GGTATCGCCA TGGGAATCAC TGGAACTGAG GTTGCCAAAG AAGCTTCGGA TATGGTTCTT 
GCAGATGATA ATTTCAGTAC TATAGTATCA GCTGTAGCAG AAGGTCGGTC CATTTACAAC 
AATATGAAAG CTTTTATCAG GTATATGATA TCATCAAACG TTGGAGAAGT AATCTCCATC 
TTCTTAACCG CGGCATTGGG GATACCGGAA TGTATGATAC CGGTTCAGCT TCTTTGGGTT 
AATCTTGTAA CTGATGGTCC TCCTGCTACT GCGTTAGGCT TCAATCCAGC TGATATTGAT 
ATCATGAAGA AGCCACCCCG CAAAAGCGAT GATTGTCTCA TTGATTCATG GGTTCTTATT 
CGATATCTAG TGATTGGTTC TTATGTTGGA GTTGCAACTG TTGGAATCTT TGTCTTGTGG 
TACACACAAG CTTCATTCCT TGGGATTAGC CTAATCTCAG ATGGACACAC ATTGGTTAGC 
TTCACTCAGC TTCAGAACTG GTCAGAGTGT TCTTCGTGGG GAACGAACTT CACGGCGACT 
CCTTACACAG TCGCAGGTGG TCTTAGGACC ATCGCGTTTG AGAACAATCC TTGCGATTAC 
TTCACTCTCG GGAAAGTCAA GCCAATGACA TTATCACTCA CCGTTCTTGT GGCTATCGAA 
ATGTTTAACT CGCTGAACGC GTTGTCTGAA GACAACAGTC TCTTAACGAT GCCACCATGG 
AGAAACCCTT GGCTACTAGT GGCCATGACT GTCTCCTTTG CGCTACACTG TGTCATCCTC 
TATGTTCCTT TCTTAGCTAA TGTGTTTGGG ATTGTGCCTT TGAGTTTCAG GGAATGGTTT 
GTGGTTATTC TTGTTTCCTT CCCTGTGATT CTGATCGATG AAGCTCTCAA GTTCATTGGG 
AGATGCAGAA GGACAAGAAT CAAGAAGAAG ATCAAGACAA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_191999.1
CDS	429..3593
Translation	MEEEKSFSAWSWSVEQCLKEYKTRLDKGLTSEDVQIRRQKYGFNELAKEKGKPLWHLVLEQFDDTLVKILLGAAFISFVLAFLGEEHGSGSGFEAFVEPFVIVLILILNAVVGVWQESNAEKALEALKEMQCESAKVLRDGNVLPNLPARELVPGDIVELNVGDKVPADMRVSGLKTSTLRVEQSSLTGEAMPVLKGANLVVMDDCELQGKENMVFAGTTVVNGSCVCIVTSIGMDTEIGKIQRQIHEASLEESETPLKKKLDEFGSRLTTAICIVCVLVWMINYKNFVSWDVVDGYKPVNIKFSFEKCTYYFKIAVALAVAAIPEGLPAVITTCLALGTRKMAQKNAIVRKLPSVETLGCTTVICSDKTGTLTTNQMSATEFFTLGGKTTTTRVFSVSGTTYDPKDGGIVDWGCNNMDANLQAVAEICSICNDAGVFYEGKLFRATGLPTEAALKVLVEKMGIPEKKNSENIEEVTNFSDNGSSVKLACCDWWNKRSKKVATLEFDRVRKSMSVIVSEPNGQNRLLVKGAAESILERSSFAQLADGSLVALDESSREVILKKHSEMTSKGLRCLGLAYKDELGEFSDYSSEEHPSHKKLLDPSSYSNIETNLIFVGVVGLRDPPREEVGRAIEDCRDAGIRVMVITGDNKSTAEAICCEIRLFSENEDLSQSSFTGKEFMSLPASRRSEILSKSGGKVFSRAEPRHKQEIVRMLKEMGEIVAMTGDGVNDAPALKLADIGIAMGITGTEVAKEASDMVLADDNFSTIVSAVAEGRSIYNNMKAFIRYMISSNVGEVISIFLTAALGIPECMIPVQLLWVNLVTDGPPATALGFNPADIDIMKKPPRKSDDCLIDSWVLIRYLVIGSYVGVATVGIFVLWYTQASFLGISLISDGHTLVSFTQLQNWSECSSWGTNFTATPYTVAGGLRTIAFENNPCDYFTLGKVKPMTLSLTVLVAIEMFNSLNALSEDNSLLTMPPWRNPWLLVAMTVSFALHCVILYVPFLANVFGIVPLSFREWFVVILVSFPVILIDEALKFIGRCRRTRIKKKIKTM

Target ORF information:

RefSeq Version	NM_116317.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana ER-type Ca2+-ATPase 2 (ECA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_116317.4

ORF Insert Sequence:

ATGGAGGAGG AGAAGTCGTT CTCGGCGTGG TCTTGGTCTG TTGAGCAATG TTTGAAAGAG 
TACAAGACAA GATTAGACAA GGGTTTAACT AGCGAAGATG TCCAAATCCG CCGTCAAAAG 
TACGGTTTTA ACGAGCTTGC TAAGGAGAAA GGCAAGCCTT TATGGCATCT TGTATTGGAA 
CAATTCGACG ATACGCTTGT GAAGATTCTT CTCGGTGCTG CGTTTATCTC TTTCGTTTTA 
GCCTTTTTAG GTGAGGAACA CGGCTCAGGT TCAGGGTTCG AGGCCTTTGT TGAGCCTTTT 
GTGATTGTTT TGATTCTGAT TTTAAACGCT GTGGTTGGTG TGTGGCAAGA GAGTAATGCT 
GAGAAAGCAC TTGAGGCTCT TAAAGAGATG CAATGTGAAT CTGCAAAGGT TTTGAGAGAT 
GGGAATGTGT TGCCTAATTT GCCTGCTAGA GAGCTTGTTC CAGGGGATAT TGTGGAGTTA 
AATGTAGGAG ATAAAGTCCC TGCTGATATG AGAGTTTCCG GTTTGAAAAC ATCCACTTTA 
AGAGTCGAGC AAAGCTCGTT AACCGGTGAA GCAATGCCGG TTTTGAAAGG AGCGAATCTT 
GTAGTCATGG ATGATTGTGA ACTGCAAGGG AAAGAAAATA TGGTTTTCGC TGGAACAACG 
GTTGTTAACG GAAGCTGCGT TTGCATTGTG ACAAGTATAG GTATGGATAC GGAGATCGGG 
AAAATCCAGA GACAGATTCA CGAGGCGTCG CTTGAAGAGA GCGAGACTCC GTTAAAGAAG 
AAGCTAGACG AGTTTGGGAG TAGATTGACT ACAGCTATTT GCATTGTCTG TGTTCTTGTG 
TGGATGATAA ACTACAAGAA CTTTGTTTCT TGGGATGTTG TGGATGGCTA TAAACCTGTG 
AATATCAAGT TTTCATTCGA GAAATGTACT TACTACTTCA AAATCGCTGT TGCTCTCGCA 
GTGGCAGCGA TACCCGAGGG TTTACCCGCT GTGATCACGA CATGTTTAGC TTTAGGGACG 
AGGAAAATGG CGCAGAAGAA CGCGATAGTG AGGAAGCTCC CGAGTGTAGA GACACTCGGT 
TGCACAACTG TGATCTGTTC AGATAAAACC GGGACTTTAA CAACAAACCA GATGTCTGCA 
ACCGAATTCT TCACATTAGG CGGTAAAACA ACGACTACTC GAGTGTTTTC AGTCAGTGGT 
ACGACTTATG ATCCTAAAGA TGGTGGAATT GTGGATTGGG GCTGCAACAA TATGGATGCT 
AACTTGCAAG CTGTTGCTGA GATATGTTCA ATTTGTAATG ATGCTGGAGT GTTCTATGAA 
GGGAAGTTGT TTAGAGCAAC AGGTTTGCCT ACAGAAGCAG CATTGAAAGT TCTTGTTGAG 
AAGATGGGTA TCCCGGAGAA GAAGAACAGT GAGAACATCG AGGAAGTTAC AAACTTTTCA 
GACAATGGTA GCTCAGTGAA GCTAGCTTGT TGCGATTGGT GGAACAAAAG ATCGAAAAAG 
GTAGCGACAT TAGAGTTTGA TCGCGTTCGT AAGTCCATGA GTGTTATTGT GAGCGAACCA 
AATGGACAAA ACCGGCTTCT CGTTAAGGGT GCTGCTGAGA GTATACTTGA GAGAAGTTCT 
TTTGCACAGC TTGCTGATGG ATCTCTTGTA GCTTTAGATG AATCTAGCAG AGAAGTAATC 
CTTAAGAAAC ATTCTGAAAT GACTTCAAAA GGATTAAGAT GTTTAGGATT AGCTTACAAA 
GACGAATTAG GAGAGTTTTC GGATTACTCT TCCGAAGAAC ATCCTTCACA CAAGAAGCTT 
TTGGACCCTT CTAGCTATTC GAACATCGAA ACAAATCTAA TCTTTGTTGG AGTTGTTGGT 
CTAAGAGATC CTCCGCGTGA AGAAGTTGGA AGAGCAATTG AAGATTGCAG AGACGCAGGG 
ATAAGAGTAA TGGTTATAAC TGGAGATAAC AAATCAACAG CTGAAGCTAT TTGTTGTGAA 
ATTAGATTGT TTTCGGAGAA TGAAGATCTT TCGCAGAGTA GCTTCACTGG TAAAGAGTTT 
ATGTCTCTTC CGGCTTCACG GAGATCCGAG ATTCTGTCAA AATCTGGAGG GAAAGTGTTC 
TCTCGAGCTG AGCCAAGGCA TAAGCAAGAA ATCGTTAGGA TGCTTAAGGA AATGGGAGAA 
ATAGTTGCCA TGACAGGTGA TGGTGTGAAT GATGCTCCTG CTTTGAAACT TGCTGACATT 
GGTATCGCCA TGGGAATCAC TGGAACTGAG GTTGCCAAAG AAGCTTCGGA TATGGTTCTT 
GCAGATGATA ATTTCAGTAC TATAGTATCA GCTGTAGCAG AAGGTCGGTC CATTTACAAC 
AATATGAAAG CTTTTATCAG GTATATGATA TCATCAAACG TTGGAGAAGT AATCTCCATC 
TTCTTAACCG CGGCATTGGG GATACCGGAA TGTATGATAC CGGTTCAGCT TCTTTGGGTT 
AATCTTGTAA CTGATGGTCC TCCTGCTACT GCGTTAGGCT TCAATCCAGC TGATATTGAT 
ATCATGAAGA AGCCACCCCG CAAAAGCGAT GATTGTCTCA TTGATTCATG GGTTCTTATT 
CGATATCTAG TGATTGGTTC TTATGTTGGA GTTGCAACTG TTGGAATCTT TGTCTTGTGG 
TACACACAAG CTTCATTCCT TGGGATTAGC CTAATCTCAG ATGGACACAC ATTGGTTAGC 
TTCACTCAGC TTCAGAACTG GTCAGAGTGT TCTTCGTGGG GAACGAACTT CACGGCGACT 
CCTTACACAG TCGCAGGTGG TCTTAGGACC ATCGCGTTTG AGAACAATCC TTGCGATTAC 
TTCACTCTCG GGAAAGTCAA GCCAATGACA TTATCACTCA CCGTTCTTGT GGCTATCGAA 
ATGTTTAACT CGCTGAACGC GTTGTCTGAA GACAACAGTC TCTTAACGAT GCCACCATGG 
AGAAACCCTT GGCTACTAGT GGCCATGACT GTCTCCTTTG CGCTACACTG TGTCATCCTC 
TATGTTCCTT TCTTAGCTAA TGTGTTTGGG ATTGTGCCTT TGAGTTTCAG GGAATGGTTT 
GTGGTTATTC TTGTTTCCTT CCCTGTGATT CTGATCGATG AAGCTCTCAA GTTCATTGGG 
AGATGCAGAA GGACAAGAAT CAAGAAGAAG ATCAAGACAA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECA2 ( NM_001340276.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001329712.1 Arabidopsis thaliana ER-type Ca2+-ATPase 2 (ECA2), mRNA.

Price & Availability↑

CloneID	OAb15645
Clone ID Related Accession (Same CDS sequence)	NM_116317.4 , NM_001340276.1
Accession Version	NM_001340276.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	ER-type Ca2+-ATPase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGGAGGAGG AGAAGTCGTT CTCGGCGTGG TCTTGGTCTG TTGAGCAATG TTTGAAAGAG 
TACAAGACAA GATTAGACAA GGGTTTAACT AGCGAAGATG TCCAAATCCG CCGTCAAAAG 
TACGGTTTTA ACGAGCTTGC TAAGGAGAAA GGCAAGCCTT TATGGCATCT TGTATTGGAA 
CAATTCGACG ATACGCTTGT GAAGATTCTT CTCGGTGCTG CGTTTATCTC TTTCGTTTTA 
GCCTTTTTAG GTGAGGAACA CGGCTCAGGT TCAGGGTTCG AGGCCTTTGT TGAGCCTTTT 
GTGATTGTTT TGATTCTGAT TTTAAACGCT GTGGTTGGTG TGTGGCAAGA GAGTAATGCT 
GAGAAAGCAC TTGAGGCTCT TAAAGAGATG CAATGTGAAT CTGCAAAGGT TTTGAGAGAT 
GGGAATGTGT TGCCTAATTT GCCTGCTAGA GAGCTTGTTC CAGGGGATAT TGTGGAGTTA 
AATGTAGGAG ATAAAGTCCC TGCTGATATG AGAGTTTCCG GTTTGAAAAC ATCCACTTTA 
AGAGTCGAGC AAAGCTCGTT AACCGGTGAA GCAATGCCGG TTTTGAAAGG AGCGAATCTT 
GTAGTCATGG ATGATTGTGA ACTGCAAGGG AAAGAAAATA TGGTTTTCGC TGGAACAACG 
GTTGTTAACG GAAGCTGCGT TTGCATTGTG ACAAGTATAG GTATGGATAC GGAGATCGGG 
AAAATCCAGA GACAGATTCA CGAGGCGTCG CTTGAAGAGA GCGAGACTCC GTTAAAGAAG 
AAGCTAGACG AGTTTGGGAG TAGATTGACT ACAGCTATTT GCATTGTCTG TGTTCTTGTG 
TGGATGATAA ACTACAAGAA CTTTGTTTCT TGGGATGTTG TGGATGGCTA TAAACCTGTG 
AATATCAAGT TTTCATTCGA GAAATGTACT TACTACTTCA AAATCGCTGT TGCTCTCGCA 
GTGGCAGCGA TACCCGAGGG TTTACCCGCT GTGATCACGA CATGTTTAGC TTTAGGGACG 
AGGAAAATGG CGCAGAAGAA CGCGATAGTG AGGAAGCTCC CGAGTGTAGA GACACTCGGT 
TGCACAACTG TGATCTGTTC AGATAAAACC GGGACTTTAA CAACAAACCA GATGTCTGCA 
ACCGAATTCT TCACATTAGG CGGTAAAACA ACGACTACTC GAGTGTTTTC AGTCAGTGGT 
ACGACTTATG ATCCTAAAGA TGGTGGAATT GTGGATTGGG GCTGCAACAA TATGGATGCT 
AACTTGCAAG CTGTTGCTGA GATATGTTCA ATTTGTAATG ATGCTGGAGT GTTCTATGAA 
GGGAAGTTGT TTAGAGCAAC AGGTTTGCCT ACAGAAGCAG CATTGAAAGT TCTTGTTGAG 
AAGATGGGTA TCCCGGAGAA GAAGAACAGT GAGAACATCG AGGAAGTTAC AAACTTTTCA 
GACAATGGTA GCTCAGTGAA GCTAGCTTGT TGCGATTGGT GGAACAAAAG ATCGAAAAAG 
GTAGCGACAT TAGAGTTTGA TCGCGTTCGT AAGTCCATGA GTGTTATTGT GAGCGAACCA 
AATGGACAAA ACCGGCTTCT CGTTAAGGGT GCTGCTGAGA GTATACTTGA GAGAAGTTCT 
TTTGCACAGC TTGCTGATGG ATCTCTTGTA GCTTTAGATG AATCTAGCAG AGAAGTAATC 
CTTAAGAAAC ATTCTGAAAT GACTTCAAAA GGATTAAGAT GTTTAGGATT AGCTTACAAA 
GACGAATTAG GAGAGTTTTC GGATTACTCT TCCGAAGAAC ATCCTTCACA CAAGAAGCTT 
TTGGACCCTT CTAGCTATTC GAACATCGAA ACAAATCTAA TCTTTGTTGG AGTTGTTGGT 
CTAAGAGATC CTCCGCGTGA AGAAGTTGGA AGAGCAATTG AAGATTGCAG AGACGCAGGG 
ATAAGAGTAA TGGTTATAAC TGGAGATAAC AAATCAACAG CTGAAGCTAT TTGTTGTGAA 
ATTAGATTGT TTTCGGAGAA TGAAGATCTT TCGCAGAGTA GCTTCACTGG TAAAGAGTTT 
ATGTCTCTTC CGGCTTCACG GAGATCCGAG ATTCTGTCAA AATCTGGAGG GAAAGTGTTC 
TCTCGAGCTG AGCCAAGGCA TAAGCAAGAA ATCGTTAGGA TGCTTAAGGA AATGGGAGAA 
ATAGTTGCCA TGACAGGTGA TGGTGTGAAT GATGCTCCTG CTTTGAAACT TGCTGACATT 
GGTATCGCCA TGGGAATCAC TGGAACTGAG GTTGCCAAAG AAGCTTCGGA TATGGTTCTT 
GCAGATGATA ATTTCAGTAC TATAGTATCA GCTGTAGCAG AAGGTCGGTC CATTTACAAC 
AATATGAAAG CTTTTATCAG GTATATGATA TCATCAAACG TTGGAGAAGT AATCTCCATC 
TTCTTAACCG CGGCATTGGG GATACCGGAA TGTATGATAC CGGTTCAGCT TCTTTGGGTT 
AATCTTGTAA CTGATGGTCC TCCTGCTACT GCGTTAGGCT TCAATCCAGC TGATATTGAT 
ATCATGAAGA AGCCACCCCG CAAAAGCGAT GATTGTCTCA TTGATTCATG GGTTCTTATT 
CGATATCTAG TGATTGGTTC TTATGTTGGA GTTGCAACTG TTGGAATCTT TGTCTTGTGG 
TACACACAAG CTTCATTCCT TGGGATTAGC CTAATCTCAG ATGGACACAC ATTGGTTAGC 
TTCACTCAGC TTCAGAACTG GTCAGAGTGT TCTTCGTGGG GAACGAACTT CACGGCGACT 
CCTTACACAG TCGCAGGTGG TCTTAGGACC ATCGCGTTTG AGAACAATCC TTGCGATTAC 
TTCACTCTCG GGAAAGTCAA GCCAATGACA TTATCACTCA CCGTTCTTGT GGCTATCGAA 
ATGTTTAACT CGCTGAACGC GTTGTCTGAA GACAACAGTC TCTTAACGAT GCCACCATGG 
AGAAACCCTT GGCTACTAGT GGCCATGACT GTCTCCTTTG CGCTACACTG TGTCATCCTC 
TATGTTCCTT TCTTAGCTAA TGTGTTTGGG ATTGTGCCTT TGAGTTTCAG GGAATGGTTT 
GTGGTTATTC TTGTTTCCTT CCCTGTGATT CTGATCGATG AAGCTCTCAA GTTCATTGGG 
AGATGCAGAA GGACAAGAAT CAAGAAGAAG ATCAAGACAA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001329712.1
CDS	254..3418
Translation	MEEEKSFSAWSWSVEQCLKEYKTRLDKGLTSEDVQIRRQKYGFNELAKEKGKPLWHLVLEQFDDTLVKILLGAAFISFVLAFLGEEHGSGSGFEAFVEPFVIVLILILNAVVGVWQESNAEKALEALKEMQCESAKVLRDGNVLPNLPARELVPGDIVELNVGDKVPADMRVSGLKTSTLRVEQSSLTGEAMPVLKGANLVVMDDCELQGKENMVFAGTTVVNGSCVCIVTSIGMDTEIGKIQRQIHEASLEESETPLKKKLDEFGSRLTTAICIVCVLVWMINYKNFVSWDVVDGYKPVNIKFSFEKCTYYFKIAVALAVAAIPEGLPAVITTCLALGTRKMAQKNAIVRKLPSVETLGCTTVICSDKTGTLTTNQMSATEFFTLGGKTTTTRVFSVSGTTYDPKDGGIVDWGCNNMDANLQAVAEICSICNDAGVFYEGKLFRATGLPTEAALKVLVEKMGIPEKKNSENIEEVTNFSDNGSSVKLACCDWWNKRSKKVATLEFDRVRKSMSVIVSEPNGQNRLLVKGAAESILERSSFAQLADGSLVALDESSREVILKKHSEMTSKGLRCLGLAYKDELGEFSDYSSEEHPSHKKLLDPSSYSNIETNLIFVGVVGLRDPPREEVGRAIEDCRDAGIRVMVITGDNKSTAEAICCEIRLFSENEDLSQSSFTGKEFMSLPASRRSEILSKSGGKVFSRAEPRHKQEIVRMLKEMGEIVAMTGDGVNDAPALKLADIGIAMGITGTEVAKEASDMVLADDNFSTIVSAVAEGRSIYNNMKAFIRYMISSNVGEVISIFLTAALGIPECMIPVQLLWVNLVTDGPPATALGFNPADIDIMKKPPRKSDDCLIDSWVLIRYLVIGSYVGVATVGIFVLWYTQASFLGISLISDGHTLVSFTQLQNWSECSSWGTNFTATPYTVAGGLRTIAFENNPCDYFTLGKVKPMTLSLTVLVAIEMFNSLNALSEDNSLLTMPPWRNPWLLVAMTVSFALHCVILYVPFLANVFGIVPLSFREWFVVILVSFPVILIDEALKFIGRCRRTRIKKKIKTM

Target ORF information:

RefSeq Version	NM_001340276.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana ER-type Ca2+-ATPase 2 (ECA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001340276.1

ORF Insert Sequence:

ATGGAGGAGG AGAAGTCGTT CTCGGCGTGG TCTTGGTCTG TTGAGCAATG TTTGAAAGAG 
TACAAGACAA GATTAGACAA GGGTTTAACT AGCGAAGATG TCCAAATCCG CCGTCAAAAG 
TACGGTTTTA ACGAGCTTGC TAAGGAGAAA GGCAAGCCTT TATGGCATCT TGTATTGGAA 
CAATTCGACG ATACGCTTGT GAAGATTCTT CTCGGTGCTG CGTTTATCTC TTTCGTTTTA 
GCCTTTTTAG GTGAGGAACA CGGCTCAGGT TCAGGGTTCG AGGCCTTTGT TGAGCCTTTT 
GTGATTGTTT TGATTCTGAT TTTAAACGCT GTGGTTGGTG TGTGGCAAGA GAGTAATGCT 
GAGAAAGCAC TTGAGGCTCT TAAAGAGATG CAATGTGAAT CTGCAAAGGT TTTGAGAGAT 
GGGAATGTGT TGCCTAATTT GCCTGCTAGA GAGCTTGTTC CAGGGGATAT TGTGGAGTTA 
AATGTAGGAG ATAAAGTCCC TGCTGATATG AGAGTTTCCG GTTTGAAAAC ATCCACTTTA 
AGAGTCGAGC AAAGCTCGTT AACCGGTGAA GCAATGCCGG TTTTGAAAGG AGCGAATCTT 
GTAGTCATGG ATGATTGTGA ACTGCAAGGG AAAGAAAATA TGGTTTTCGC TGGAACAACG 
GTTGTTAACG GAAGCTGCGT TTGCATTGTG ACAAGTATAG GTATGGATAC GGAGATCGGG 
AAAATCCAGA GACAGATTCA CGAGGCGTCG CTTGAAGAGA GCGAGACTCC GTTAAAGAAG 
AAGCTAGACG AGTTTGGGAG TAGATTGACT ACAGCTATTT GCATTGTCTG TGTTCTTGTG 
TGGATGATAA ACTACAAGAA CTTTGTTTCT TGGGATGTTG TGGATGGCTA TAAACCTGTG 
AATATCAAGT TTTCATTCGA GAAATGTACT TACTACTTCA AAATCGCTGT TGCTCTCGCA 
GTGGCAGCGA TACCCGAGGG TTTACCCGCT GTGATCACGA CATGTTTAGC TTTAGGGACG 
AGGAAAATGG CGCAGAAGAA CGCGATAGTG AGGAAGCTCC CGAGTGTAGA GACACTCGGT 
TGCACAACTG TGATCTGTTC AGATAAAACC GGGACTTTAA CAACAAACCA GATGTCTGCA 
ACCGAATTCT TCACATTAGG CGGTAAAACA ACGACTACTC GAGTGTTTTC AGTCAGTGGT 
ACGACTTATG ATCCTAAAGA TGGTGGAATT GTGGATTGGG GCTGCAACAA TATGGATGCT 
AACTTGCAAG CTGTTGCTGA GATATGTTCA ATTTGTAATG ATGCTGGAGT GTTCTATGAA 
GGGAAGTTGT TTAGAGCAAC AGGTTTGCCT ACAGAAGCAG CATTGAAAGT TCTTGTTGAG 
AAGATGGGTA TCCCGGAGAA GAAGAACAGT GAGAACATCG AGGAAGTTAC AAACTTTTCA 
GACAATGGTA GCTCAGTGAA GCTAGCTTGT TGCGATTGGT GGAACAAAAG ATCGAAAAAG 
GTAGCGACAT TAGAGTTTGA TCGCGTTCGT AAGTCCATGA GTGTTATTGT GAGCGAACCA 
AATGGACAAA ACCGGCTTCT CGTTAAGGGT GCTGCTGAGA GTATACTTGA GAGAAGTTCT 
TTTGCACAGC TTGCTGATGG ATCTCTTGTA GCTTTAGATG AATCTAGCAG AGAAGTAATC 
CTTAAGAAAC ATTCTGAAAT GACTTCAAAA GGATTAAGAT GTTTAGGATT AGCTTACAAA 
GACGAATTAG GAGAGTTTTC GGATTACTCT TCCGAAGAAC ATCCTTCACA CAAGAAGCTT 
TTGGACCCTT CTAGCTATTC GAACATCGAA ACAAATCTAA TCTTTGTTGG AGTTGTTGGT 
CTAAGAGATC CTCCGCGTGA AGAAGTTGGA AGAGCAATTG AAGATTGCAG AGACGCAGGG 
ATAAGAGTAA TGGTTATAAC TGGAGATAAC AAATCAACAG CTGAAGCTAT TTGTTGTGAA 
ATTAGATTGT TTTCGGAGAA TGAAGATCTT TCGCAGAGTA GCTTCACTGG TAAAGAGTTT 
ATGTCTCTTC CGGCTTCACG GAGATCCGAG ATTCTGTCAA AATCTGGAGG GAAAGTGTTC 
TCTCGAGCTG AGCCAAGGCA TAAGCAAGAA ATCGTTAGGA TGCTTAAGGA AATGGGAGAA 
ATAGTTGCCA TGACAGGTGA TGGTGTGAAT GATGCTCCTG CTTTGAAACT TGCTGACATT 
GGTATCGCCA TGGGAATCAC TGGAACTGAG GTTGCCAAAG AAGCTTCGGA TATGGTTCTT 
GCAGATGATA ATTTCAGTAC TATAGTATCA GCTGTAGCAG AAGGTCGGTC CATTTACAAC 
AATATGAAAG CTTTTATCAG GTATATGATA TCATCAAACG TTGGAGAAGT AATCTCCATC 
TTCTTAACCG CGGCATTGGG GATACCGGAA TGTATGATAC CGGTTCAGCT TCTTTGGGTT 
AATCTTGTAA CTGATGGTCC TCCTGCTACT GCGTTAGGCT TCAATCCAGC TGATATTGAT 
ATCATGAAGA AGCCACCCCG CAAAAGCGAT GATTGTCTCA TTGATTCATG GGTTCTTATT 
CGATATCTAG TGATTGGTTC TTATGTTGGA GTTGCAACTG TTGGAATCTT TGTCTTGTGG 
TACACACAAG CTTCATTCCT TGGGATTAGC CTAATCTCAG ATGGACACAC ATTGGTTAGC 
TTCACTCAGC TTCAGAACTG GTCAGAGTGT TCTTCGTGGG GAACGAACTT CACGGCGACT 
CCTTACACAG TCGCAGGTGG TCTTAGGACC ATCGCGTTTG AGAACAATCC TTGCGATTAC 
TTCACTCTCG GGAAAGTCAA GCCAATGACA TTATCACTCA CCGTTCTTGT GGCTATCGAA 
ATGTTTAACT CGCTGAACGC GTTGTCTGAA GACAACAGTC TCTTAACGAT GCCACCATGG 
AGAAACCCTT GGCTACTAGT GGCCATGACT GTCTCCTTTG CGCTACACTG TGTCATCCTC 
TATGTTCCTT TCTTAGCTAA TGTGTTTGGG ATTGTGCCTT TGAGTTTCAG GGAATGGTTT 
GTGGTTATTC TTGTTTCCTT CCCTGTGATT CTGATCGATG AAGCTCTCAA GTTCATTGGG 
AGATGCAGAA GGACAAGAAT CAAGAAGAAG ATCAAGACAA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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ECA2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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