AT4G16745 cDNA ORF clone, Arabidopsis thaliana(thale cress)

The following AT4G16745 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT4G16745 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OAb15127 NM_117776.5
Latest version!
Arabidopsis thaliana Exostosin family protein (AT4G16745), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OAb29574 NM_001203814.1
Latest version!
Arabidopsis thaliana Exostosin family protein (AT4G16745), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OAb15127
    Clone ID Related Accession (Same CDS sequence) NM_117776.5
    Accession Version NM_117776.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product Exostosin family protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_117776.4.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGTGAATA TTAAAAGCCC TTTACGGCTA TTCATAGAGG AGTCGATTAA GGAATTAATT 
    AGGGGTTTCA GGAGACAAAG GATCAAATGG AGAAATGCGT TTCTTCTTGG TTCTATAATG
    ACTACCATTG TTGTTCTGCT TCATACACCA ACGTTTTCAG TCTTCTCTGA TGAAGAAGAA
    ACCGAGTCTT CTTCTTCTTC GCCTATTTAC TTGAATGGTT CTCTGCATTT GAATATCCAT
    ATTGTTAGTA GTGAAGCAAA AGTTGAAAAT TTTCATACTC TTAGAACAAG AACTCCTATT
    GTGCAATTGA ATGCTTCTGA AGCAAGTGAA GCTGTTCTTT CGAGAAAAAG GCGAAAAAGG
    AAGAAAAGAA AGAAGACAAA AGATGATCTG ATTCTCACTG ATCCTCCACC AGCACCACGA
    CATGTTCTAT CTTCCTCAGA GAGACGTGCT CTTTCCTTGC CACCGAAGAA AGCTCTTACT
    TATGCAAAAC TGGAGATTCA GCGTGCCCCG GAGGTCATAA ATGATACAGA TCTGTTTGCC
    CCATTGTTTC GAAATCTCTC TGTTTTCAAA AGGAGCTATG AGCTTATGGA ACTAATACTA
    AAGGTCTACA TATATCCTGA CGGAGACAAA CCCATCTTCC ACGAACCGCA TTTAAATGGT
    ATATATGCTT CAGAAGGTTG GTTTATGAAG CTAATGGAGT CAAACAAACA GTTTGTCACA
    AAGAACCCTG AGAGGGCTCA CTTGTTCTAC ATGCCATATA GTGTGAAACA GCTTCAGAAA
    AGTATCTTTG TTCCTGGATC ACATAACATC AAACCTTTAT CGATCTTTCT TAGAGACTAC
    GTCAACATGC TCTCCATCAA ATACCCCTTC TGGAACCGCA CTCATGGGTC AGATCATTTC
    CTTGTCGCTT GCCACGATTG GGGTCCTTAC ACAGTGAACG AGCACCCAGA GCTAAAACGA
    AACGCTATTA AAGCTCTCTG CAATGCAGAC TTATCAGATG GAATCTTCGT CCCGGGGAAA
    GACGTTTCTC TCCCTGAAAC ATCCATAAGA AACGCAGGTA GACCACTACG CAACATAGGA
    AACGGAAACA GAGTTTCTCA ACGTCCCATC CTCGCTTTCT TCGCTGGAAA CCTCCACGGT
    CGTGTCCGTC CAAAGCTTCT AAAACACTGG AGAAACAAAG ACGAGGACAT GAAAATCTAC
    GGTCCGCTTC CACACAACGT AGCTAGGAAA ATGACTTACG TACAACACAT GAAATCAAGC
    AAGTACTGTC TTTGTCCAAT GGGTTACGAA GTGAACAGTC CACGTATCGT TGAAGCTATA
    TACTATGAAT GCGTCCCTGT AGTGATCGCT GATAACTTCA TGTTACCGTT CAGCGATGTG
    CTAGATTGGT CAGCGTTCTC AGTGGTTGTA CCGGAGAAGG AGATTCCACG GCTGAAGGAG
    ATACTATTGG AGATTCCTAT GAGGAGATAT TTGAAGATGC AGAGTAACGT GAAGATGGTT
    CAGAGACATT TCTTGTGGAG CCCTAAACCT AGAAAGTACG ATGTGTTTCA TATGATACTT
    CACTCCATTT GGTTTAACCT TCTCAATCAA AACCAAACTT CTTTTGATGA TCATATTCCC
    GATTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_567512.2
    CDS269..1897
    Translation

    Target ORF information:

    RefSeq Version NM_117776.5
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana Exostosin family protein (AT4G16745), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_117776.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGTGAATA TTAAAAGCCC TTTACGGCTA TTCATAGAGG AGTCGATTAA GGAATTAATT 
    AGGGGTTTCA GGAGACAAAG GATCAAATGG AGAAATGCGT TTCTTCTTGG TTCTATAATG
    ACTACCATTG TTGTTCTGCT TCATACACCA ACGTTTTCAG TCTTCTCTGA TGAAGAAGAA
    ACCGAGTCTT CTTCTTCTTC GCCTATTTAC TTGAATGGTT CTCTGCATTT GAATATCCAT
    ATTGTTAGTA GTGAAGCAAA AGTTGAAAAT TTTCATACTC TTAGAACAAG AACTCCTATT
    GTGCAATTGA ATGCTTCTGA AGCAAGTGAA GCTGTTCTTT CGAGAAAAAG GCGAAAAAGG
    AAGAAAAGAA AGAAGACAAA AGATGATCTG ATTCTCACTG ATCCTCCACC AGCACCACGA
    CATGTTCTAT CTTCCTCAGA GAGACGTGCT CTTTCCTTGC CACCGAAGAA AGCTCTTACT
    TATGCAAAAC TGGAGATTCA GCGTGCCCCG GAGGTCATAA ATGATACAGA TCTGTTTGCC
    CCATTGTTTC GAAATCTCTC TGTTTTCAAA AGGAGCTATG AGCTTATGGA ACTAATACTA
    AAGGTCTACA TATATCCTGA CGGAGACAAA CCCATCTTCC ACGAACCGCA TTTAAATGGT
    ATATATGCTT CAGAAGGTTG GTTTATGAAG CTAATGGAGT CAAACAAACA GTTTGTCACA
    AAGAACCCTG AGAGGGCTCA CTTGTTCTAC ATGCCATATA GTGTGAAACA GCTTCAGAAA
    AGTATCTTTG TTCCTGGATC ACATAACATC AAACCTTTAT CGATCTTTCT TAGAGACTAC
    GTCAACATGC TCTCCATCAA ATACCCCTTC TGGAACCGCA CTCATGGGTC AGATCATTTC
    CTTGTCGCTT GCCACGATTG GGGTCCTTAC ACAGTGAACG AGCACCCAGA GCTAAAACGA
    AACGCTATTA AAGCTCTCTG CAATGCAGAC TTATCAGATG GAATCTTCGT CCCGGGGAAA
    GACGTTTCTC TCCCTGAAAC ATCCATAAGA AACGCAGGTA GACCACTACG CAACATAGGA
    AACGGAAACA GAGTTTCTCA ACGTCCCATC CTCGCTTTCT TCGCTGGAAA CCTCCACGGT
    CGTGTCCGTC CAAAGCTTCT AAAACACTGG AGAAACAAAG ACGAGGACAT GAAAATCTAC
    GGTCCGCTTC CACACAACGT AGCTAGGAAA ATGACTTACG TACAACACAT GAAATCAAGC
    AAGTACTGTC TTTGTCCAAT GGGTTACGAA GTGAACAGTC CACGTATCGT TGAAGCTATA
    TACTATGAAT GCGTCCCTGT AGTGATCGCT GATAACTTCA TGTTACCGTT CAGCGATGTG
    CTAGATTGGT CAGCGTTCTC AGTGGTTGTA CCGGAGAAGG AGATTCCACG GCTGAAGGAG
    ATACTATTGG AGATTCCTAT GAGGAGATAT TTGAAGATGC AGAGTAACGT GAAGATGGTT
    CAGAGACATT TCTTGTGGAG CCCTAAACCT AGAAAGTACG ATGTGTTTCA TATGATACTT
    CACTCCATTT GGTTTAACCT TCTCAATCAA AACCAAACTT CTTTTGATGA TCATATTCCC
    GATTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OAb29574
    Clone ID Related Accession (Same CDS sequence) NM_001203814.1
    Accession Version NM_001203814.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1770bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product Exostosin family protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGTGAATA TTAAAAGCCC TTTACGGCTA TTCATAGAGG AGTCGATTAA GGAATTAATT 
    AGGGGTTTCA GGAGACAAAG GATCAAATGG AGAAATGCGT TTCTTCTTGG TTCTATAATG
    ACTACCATTG TTGTTCTGCT TCATACACCA ACGTTTTCAG TCTTCTCTGA TGAAGAAGAA
    ACCGAGTCTT CTTCTTCTTC GCCTATTTAC TTGAATGGTT CTCTGCATTT GAATATCCAT
    ATTGTTAGTA GTGAAGCAAA AGTTGAAAAT TTTCATACTC TTAGAACAAG AACTCCTATT
    GTGCAATTGA ATGCTTCTGA AGCAAGTGAA GCTGTTCTTT CGAGAAAAAG GCGAAAAAGG
    AAGAAAAGAA AGAAGACAAA AGATGATCTG ATTCTCACTG ATCCTCCACC AGCACCACGA
    CATGTTCTAT CTTCCTCAGA GAGACGTGCT CTTTCCTTGC CACCGAAGAA AGCTCTTACT
    TATGCAAAAC TGGAGATTCA GCGTGCCCCG GAGGTCATAA ATGATACAGA TCTGTTTGCC
    CCATTGTTTC GAAATCTCTC TGTTTTCAAA AGGAGCTATG AGCTTATGGA ACTAATACTA
    AAGGTCTACA TATATCCTGA CGGAGACAAA CCCATCTTCC ACGAACCGCA TTTAAATGGT
    ATATATGCTT CAGAAGGTTG GTTTATGAAG CTAATGGAGT CAAACAAACA GTTTGTCACA
    AAGAACCCTG AGAGGGCTCA CTTGTTCTAC ATGCCATATA GTGTGAAACA GCTTCAGAAA
    AGTATCTTTG TTCCTGGATC ACATAACATC AAACCTTTAT CGATCTTTCT TAGAGACTAC
    GTCAACATGC TCTCCATCAA ATACCCCTTC TGGAACCGCA CTCATGGGTC AGATCATTTC
    CTTGTCGCTT GCCACGATTG GGGTCCTTAC ACAGTGAACG AGCACCCAGA GCTAAAACGA
    AACGCTATTA AAGCTCTCTG CAATGCAGAC TTATCAGATG GAATCTTCGT CCCGGGGAAA
    GACGTTTCTC TCCCTGAAAC ATCCATAAGA AACGCAGGTA GACCACTACG CAACATAGGA
    AACGGAAACA GAGTTTCTCA ACGTCCCATC CTCGCTTTCT TCGCTGGAAA CCTCCACGGT
    CGTGTCCGTC CAAAGCTTCT AAAACACTGG AGAAACAAAG ACGAGGACAT GAAAATCTAC
    GGTCCGCTTC CACACAACGT AGCTAGGAAA ATGACTTACG TACAACACAT GAAATCAAGC
    AAGTACTGTC TTTGTCCAAT GGGTTACGAA GTGAACAGTC CACGTATCGT TGAAGCTATA
    TACTATGAAT GCGTCCCTGT AGTGATCGCT GATAACTTCA TGTTACCGTT CAGCGATGTG
    CTAGATTGGT CAGCGTTCTC AGTGGTTGTA CCGGAGAAGG AGATTCCACG GCTGAAGGAG
    ATACTATTGG AGATTCCTAT GAGGAGATAT TTGAAGATGC AGAGTAACGT GAAGATGGTT
    CAGAGACATT TCTTGTGGAG CCCTAAACCT AGAAAGATTA AACCGTTTGT GAAAGAGCGC
    ATTACTATTT TAAAGCTTCT CCTTCAGCCC GCGGTTTACC ACGTTTGGAA AAAGCGCAAC
    GCTAGGATCT TCACCAGCAT TCCATCTTCG GTCTCCACTC AGTGTTTGGC AATTGATAAG
    ACCTTGAGGA ATCGTCTTTT GTCCTTTCCT TCCTCAGACA TGCGCTCTTC CACTACTCTT
    CTTCATTTTT ACTTCTCTTG TATCTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001190743.1
    CDS252..2021
    Translation

    Target ORF information:

    RefSeq Version NM_001203814.1
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana Exostosin family protein (AT4G16745), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001203814.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGTGAATA TTAAAAGCCC TTTACGGCTA TTCATAGAGG AGTCGATTAA GGAATTAATT 
    AGGGGTTTCA GGAGACAAAG GATCAAATGG AGAAATGCGT TTCTTCTTGG TTCTATAATG
    ACTACCATTG TTGTTCTGCT TCATACACCA ACGTTTTCAG TCTTCTCTGA TGAAGAAGAA
    ACCGAGTCTT CTTCTTCTTC GCCTATTTAC TTGAATGGTT CTCTGCATTT GAATATCCAT
    ATTGTTAGTA GTGAAGCAAA AGTTGAAAAT TTTCATACTC TTAGAACAAG AACTCCTATT
    GTGCAATTGA ATGCTTCTGA AGCAAGTGAA GCTGTTCTTT CGAGAAAAAG GCGAAAAAGG
    AAGAAAAGAA AGAAGACAAA AGATGATCTG ATTCTCACTG ATCCTCCACC AGCACCACGA
    CATGTTCTAT CTTCCTCAGA GAGACGTGCT CTTTCCTTGC CACCGAAGAA AGCTCTTACT
    TATGCAAAAC TGGAGATTCA GCGTGCCCCG GAGGTCATAA ATGATACAGA TCTGTTTGCC
    CCATTGTTTC GAAATCTCTC TGTTTTCAAA AGGAGCTATG AGCTTATGGA ACTAATACTA
    AAGGTCTACA TATATCCTGA CGGAGACAAA CCCATCTTCC ACGAACCGCA TTTAAATGGT
    ATATATGCTT CAGAAGGTTG GTTTATGAAG CTAATGGAGT CAAACAAACA GTTTGTCACA
    AAGAACCCTG AGAGGGCTCA CTTGTTCTAC ATGCCATATA GTGTGAAACA GCTTCAGAAA
    AGTATCTTTG TTCCTGGATC ACATAACATC AAACCTTTAT CGATCTTTCT TAGAGACTAC
    GTCAACATGC TCTCCATCAA ATACCCCTTC TGGAACCGCA CTCATGGGTC AGATCATTTC
    CTTGTCGCTT GCCACGATTG GGGTCCTTAC ACAGTGAACG AGCACCCAGA GCTAAAACGA
    AACGCTATTA AAGCTCTCTG CAATGCAGAC TTATCAGATG GAATCTTCGT CCCGGGGAAA
    GACGTTTCTC TCCCTGAAAC ATCCATAAGA AACGCAGGTA GACCACTACG CAACATAGGA
    AACGGAAACA GAGTTTCTCA ACGTCCCATC CTCGCTTTCT TCGCTGGAAA CCTCCACGGT
    CGTGTCCGTC CAAAGCTTCT AAAACACTGG AGAAACAAAG ACGAGGACAT GAAAATCTAC
    GGTCCGCTTC CACACAACGT AGCTAGGAAA ATGACTTACG TACAACACAT GAAATCAAGC
    AAGTACTGTC TTTGTCCAAT GGGTTACGAA GTGAACAGTC CACGTATCGT TGAAGCTATA
    TACTATGAAT GCGTCCCTGT AGTGATCGCT GATAACTTCA TGTTACCGTT CAGCGATGTG
    CTAGATTGGT CAGCGTTCTC AGTGGTTGTA CCGGAGAAGG AGATTCCACG GCTGAAGGAG
    ATACTATTGG AGATTCCTAT GAGGAGATAT TTGAAGATGC AGAGTAACGT GAAGATGGTT
    CAGAGACATT TCTTGTGGAG CCCTAAACCT AGAAAGATTA AACCGTTTGT GAAAGAGCGC
    ATTACTATTT TAAAGCTTCT CCTTCAGCCC GCGGTTTACC ACGTTTGGAA AAAGCGCAAC
    GCTAGGATCT TCACCAGCAT TCCATCTTCG GTCTCCACTC AGTGTTTGGC AATTGATAAG
    ACCTTGAGGA ATCGTCTTTT GTCCTTTCCT TCCTCAGACA TGCGCTCTTC CACTACTCTT
    CTTCATTTTT ACTTCTCTTG TATCTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
    Nature402(6763)769-77(1999 Dec)
    Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR