CUL1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	CUL1
Entrez Gene ID	825648
Full Name	cullin 1
Synonyms	ATCUL1,AUXIN RESISTANT 6,AXR6,T10P11.26,T10P11_26,cullin 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001036499.1	NP_001031576.1	cullin 1
NM_001036498.3	NP_001031575.1	cullin 1
NM_001203732.1	NP_001190661.1	cullin 1
NM_116491.3	NP_567243.1	cullin 1

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os01g0369000	NP_001043048.1
Danio rerio (zebrafish)	cul1a	NP_955953.2
Arabidopsis thaliana (thale cress)	CUL1	NP_001190661.1
Oryza sativa (rice)	Os05g0149600	NP_001054659.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The axr6-101 phenotype is caused by the E716K substitution of the CUL1 protein, which is likely to affect its ability to bind to the C-terminal RING domain of RING-box 1 (RBX1).
Title: A new temperature-insensitive allele of the Arabidopsis AXR6/CUL1 locus derived from a missense mutation in the C-terminal RBX1 binding region.

a new viable recessive allele of the Arabidopsis CULLIN1 gene in the non-reference Wassilewskija (Ws-4) accession, was identified.
Title: A novel viable allele of Arabidopsis CULLIN1 identified in a screen for superroot2 suppressors by next generation sequencing-assisted mapping.

icu13 is a novel recessive allele of AUXIN RESISTANT6 (AXR6), which encodes CULLIN1, an invariable component of the SCF complex.[ICU13] [ncurvata13]
Title: incurvata13, a novel allele of AUXIN RESISTANT6, reveals a specific role for auxin and the SCF complex in Arabidopsis embryogenesis, vascular specification, and leaf flatness.

CUL1-based SCF E3 ligase activity is required for Della protein degradation.
Title: Biochemical insights on degradation of Arabidopsis DELLA proteins gained from a cell-free assay system.

the first reported allele of CUL1 to directly affect subunit interactions at the CUL1 C terminus
Title: Isolation and characterization of cul1-7, a recessive allele of CULLIN1 that disrupts SCF function at the C terminus of CUL1 in Arabidopsis thaliana.

The following CUL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CUL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb14247	NM_001203732.1 Latest version!	Arabidopsis thaliana cullin 1 (CUL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb14247	NM_001036499.1 Latest version!	Arabidopsis thaliana cullin 1 (CUL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb14247	NM_116491.3 Latest version!	Arabidopsis thaliana cullin 1 (CUL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb14247	NM_001036498.3 Latest version!	Arabidopsis thaliana cullin 1 (CUL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CUL1 ( NM_001036499.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001031576.1 Arabidopsis thaliana cullin 1 (CUL1), mRNA.

Price & Availability↑

CloneID	OAb14247
Clone ID Related Accession (Same CDS sequence)	NM_001036499.1 , NM_001036498.3 , NM_001203732.1 , NM_116491.3
Accession Version	NM_001036499.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	cullin 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGGAGCGCA AGACTATTGA CTTGGAGCAA GGATGGGACT ATATGCAGAC TGGAATTACT 
AAGCTGAAAC GGATTCTGGA AGGATTGAAT GAGCCAGCAT TTGACTCTGA GCAATATATG 
ATGCTTTATA CGACTATCTA CAACATGTGC ACCCAGAAAC CTCCTCATGA TTACTCACAG 
CAGCTTTATG ACAAGTATCG GGAAGCATTT GAGGAATATA TTAACTCAAC AGTTTTGCCT 
GCGTTGAGGG AGAAGCATGA TGAGTTTATG CTGCGGGAGC TATTTAAAAG ATGGTCAAAC 
CATAAAGTAA TGGTCAGATG GCTATCCCGC TTCTTCTACT ACCTTGACCG TTACTTCATT 
GCTCGGAGAT CACTTCCACC ACTGAATGAA GTTGGCCTGA CATGCTTCCG TGACCTGGTT 
TATAATGAGC TACATTCTAA GGTCAAACAA GCTGTAATAG CTCTTGTTGA TAAAGAACGG 
GAGGGCGAGC AGATTGATAG GGCCCTGCTG AAAAACGTAT TAGATATCTA TGTAGAGATT 
GGAATGGGGC AGATGGAGAG GTATGAAGAA GATTTTGAAA GCTTCATGCT TCAAGATACT 
TCTTCGTATT ATTCTCGCAA GGCATCAAGC TGGATTCAGG AAGATTCTTG CCCTGATTAC 
ATGTTGAAGT CTGAAGAATG TCTAAAGAAG GAGAGGGAGA GAGTGGCTCA CTACCTACAC 
TCAAGCAGTG AGCCAAAGCT GGTTGAGAAA GTACAACATG AATTGCTGGT TGTGTTTGCA 
AGTCAGCTTC TAGAAAAAGA ACACTCAGGG TGCCGTGCAT TGCTAAGAGA TGACAAGGTG 
GATGATCTCT CCAGGATGTA CAGGCTTTAC CATAAAATTT TGCGAGGCTT GGAACCTGTT 
GCAAACATCT TTAAGCAGCA TGTCACAGCA GAGGGTAACG CTCTTGTCCA ACAGGCCGAA 
GACACGGCTA CTAATCAGGT TGCAAATACT GCTAGCGTCC AGGAACAGGT TCTTATCAGA 
AAAGTGATTG AACTTCATGA TAAATACATG GTATATGTCA CCGAGTGTTT CCAGAACCAC 
ACCCTCTTCC ATAAGGCTTT GAAAGAGGCA TTTGAGATTT TTTGTAACAA AACGGTTGCT 
GGAAGTTCAA GTGCAGAACT ACTTGCAACA TTTTGCGACA ATATTCTCAA AAAGGGGGGA 
AGTGAAAAGC TGAGTGATGA AGCTATCGAA GATACGCTTG AGAAGGTTGT CAAATTGCTT 
GCATACATAA GTGACAAGGA TCTTTTCGCT GAGTTCTACA GGAAGAAGCT GGCCCGTAGG 
CTCTTATTTG ATCGCAGTGC TAATGATGAT CATGAGAGAA GTATCCTGAC AAAGCTCAAG 
CAACAATGTG GTGGACAGTT TACTTCTAAG ATGGAGGGCA TGGTGACGGA TTTGACACTG 
GCAAGAGAAA ACCAAAACAG TTTCGAGGAT TATCTAGGCA GTAACCCTGC TGCAAACCCA 
GGGATTGACT TGACCGTCAC TGTTCTTACC ACTGGTTTCT GGCCAAGTTA CAAATCATTT 
GACATAAATC TACCCAGTGA AATGATCAAG TGTGTTGAAG TCTTCAAAGG GTTTTATGAA 
ACGAAAACGA AACACAGGAA GCTTACGTGG ATCTATTCAC TGGGAACTTG TCACATAAAC 
GGGAAGTTTG ATCAAAAGGC CATCGAGTTA ATAGTGTCTA CTTACCAGGC TGCTGTGCTT 
CTACTCTTTA ACACAACTGA CAAGTTAAGT TACACTGAGA TCTTGGCTCA ACTGAACCTA 
AGCCATGAAG ATCTAGTTAG GTTGCTTCAT TCCTTGTCAT GTGCTAAGTA CAAGATACTC 
CTTAAGGAGC CAAACACCAA GACTGTCTCC CAGAATGATG CCTTTGAGTT CAACTCCAAA 
TTCACCGATA GAATGCGCAG AATCAAGATC CCTCTTCCCC CAGTTGATGA AAGGAAGAAA 
GTCGTTGAAG ATGTCGATAA AGACAGAAGA TATGCAATTG ATGCTGCCAT TGTCAGGATC 
ATGAAGAGCA GGAAAGTATT GGGACATCAA CAACTTGTTT CTGAGTGTGT TGAGCAACTT 
AGCCGAATGT TCAAGCCTGA TATCAAAGCG ATCAAGAAGC GTATGGAGGA TTTAATAACC 
AGAGATTATT TGGAGAGGGA CAAGGAGAAT CCTAACATGT TTAGGTACTT GGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001031576.1
CDS	176..2392
Translation	MERKTIDLEQGWDYMQTGITKLKRILEGLNEPAFDSEQYMMLYTTIYNMCTQKPPHDYSQQLYDKYREAFEEYINSTVLPALREKHDEFMLRELFKRWSNHKVMVRWLSRFFYYLDRYFIARRSLPPLNEVGLTCFRDLVYNELHSKVKQAVIALVDKEREGEQIDRALLKNVLDIYVEIGMGQMERYEEDFESFMLQDTSSYYSRKASSWIQEDSCPDYMLKSEECLKKERERVAHYLHSSSEPKLVEKVQHELLVVFASQLLEKEHSGCRALLRDDKVDDLSRMYRLYHKILRGLEPVANIFKQHVTAEGNALVQQAEDTATNQVANTASVQEQVLIRKVIELHDKYMVYVTECFQNHTLFHKALKEAFEIFCNKTVAGSSSAELLATFCDNILKKGGSEKLSDEAIEDTLEKVVKLLAYISDKDLFAEFYRKKLARRLLFDRSANDDHERSILTKLKQQCGGQFTSKMEGMVTDLTLARENQNSFEDYLGSNPAANPGIDLTVTVLTTGFWPSYKSFDINLPSEMIKCVEVFKGFYETKTKHRKLTWIYSLGTCHINGKFDQKAIELIVSTYQAAVLLLFNTTDKLSYTEILAQLNLSHEDLVRLLHSLSCAKYKILLKEPNTKTVSQNDAFEFNSKFTDRMRRIKIPLPPVDERKKVVEDVDKDRRYAIDAAIVRIMKSRKVLGHQQLVSECVEQLSRMFKPDIKAIKKRMEDLITRDYLERDKENPNMFRYLA

Target ORF information:

RefSeq Version	NM_001036499.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana cullin 1 (CUL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001036499.1

ORF Insert Sequence:

ATGGAGCGCA AGACTATTGA CTTGGAGCAA GGATGGGACT ATATGCAGAC TGGAATTACT 
AAGCTGAAAC GGATTCTGGA AGGATTGAAT GAGCCAGCAT TTGACTCTGA GCAATATATG 
ATGCTTTATA CGACTATCTA CAACATGTGC ACCCAGAAAC CTCCTCATGA TTACTCACAG 
CAGCTTTATG ACAAGTATCG GGAAGCATTT GAGGAATATA TTAACTCAAC AGTTTTGCCT 
GCGTTGAGGG AGAAGCATGA TGAGTTTATG CTGCGGGAGC TATTTAAAAG ATGGTCAAAC 
CATAAAGTAA TGGTCAGATG GCTATCCCGC TTCTTCTACT ACCTTGACCG TTACTTCATT 
GCTCGGAGAT CACTTCCACC ACTGAATGAA GTTGGCCTGA CATGCTTCCG TGACCTGGTT 
TATAATGAGC TACATTCTAA GGTCAAACAA GCTGTAATAG CTCTTGTTGA TAAAGAACGG 
GAGGGCGAGC AGATTGATAG GGCCCTGCTG AAAAACGTAT TAGATATCTA TGTAGAGATT 
GGAATGGGGC AGATGGAGAG GTATGAAGAA GATTTTGAAA GCTTCATGCT TCAAGATACT 
TCTTCGTATT ATTCTCGCAA GGCATCAAGC TGGATTCAGG AAGATTCTTG CCCTGATTAC 
ATGTTGAAGT CTGAAGAATG TCTAAAGAAG GAGAGGGAGA GAGTGGCTCA CTACCTACAC 
TCAAGCAGTG AGCCAAAGCT GGTTGAGAAA GTACAACATG AATTGCTGGT TGTGTTTGCA 
AGTCAGCTTC TAGAAAAAGA ACACTCAGGG TGCCGTGCAT TGCTAAGAGA TGACAAGGTG 
GATGATCTCT CCAGGATGTA CAGGCTTTAC CATAAAATTT TGCGAGGCTT GGAACCTGTT 
GCAAACATCT TTAAGCAGCA TGTCACAGCA GAGGGTAACG CTCTTGTCCA ACAGGCCGAA 
GACACGGCTA CTAATCAGGT TGCAAATACT GCTAGCGTCC AGGAACAGGT TCTTATCAGA 
AAAGTGATTG AACTTCATGA TAAATACATG GTATATGTCA CCGAGTGTTT CCAGAACCAC 
ACCCTCTTCC ATAAGGCTTT GAAAGAGGCA TTTGAGATTT TTTGTAACAA AACGGTTGCT 
GGAAGTTCAA GTGCAGAACT ACTTGCAACA TTTTGCGACA ATATTCTCAA AAAGGGGGGA 
AGTGAAAAGC TGAGTGATGA AGCTATCGAA GATACGCTTG AGAAGGTTGT CAAATTGCTT 
GCATACATAA GTGACAAGGA TCTTTTCGCT GAGTTCTACA GGAAGAAGCT GGCCCGTAGG 
CTCTTATTTG ATCGCAGTGC TAATGATGAT CATGAGAGAA GTATCCTGAC AAAGCTCAAG 
CAACAATGTG GTGGACAGTT TACTTCTAAG ATGGAGGGCA TGGTGACGGA TTTGACACTG 
GCAAGAGAAA ACCAAAACAG TTTCGAGGAT TATCTAGGCA GTAACCCTGC TGCAAACCCA 
GGGATTGACT TGACCGTCAC TGTTCTTACC ACTGGTTTCT GGCCAAGTTA CAAATCATTT 
GACATAAATC TACCCAGTGA AATGATCAAG TGTGTTGAAG TCTTCAAAGG GTTTTATGAA 
ACGAAAACGA AACACAGGAA GCTTACGTGG ATCTATTCAC TGGGAACTTG TCACATAAAC 
GGGAAGTTTG ATCAAAAGGC CATCGAGTTA ATAGTGTCTA CTTACCAGGC TGCTGTGCTT 
CTACTCTTTA ACACAACTGA CAAGTTAAGT TACACTGAGA TCTTGGCTCA ACTGAACCTA 
AGCCATGAAG ATCTAGTTAG GTTGCTTCAT TCCTTGTCAT GTGCTAAGTA CAAGATACTC 
CTTAAGGAGC CAAACACCAA GACTGTCTCC CAGAATGATG CCTTTGAGTT CAACTCCAAA 
TTCACCGATA GAATGCGCAG AATCAAGATC CCTCTTCCCC CAGTTGATGA AAGGAAGAAA 
GTCGTTGAAG ATGTCGATAA AGACAGAAGA TATGCAATTG ATGCTGCCAT TGTCAGGATC 
ATGAAGAGCA GGAAAGTATT GGGACATCAA CAACTTGTTT CTGAGTGTGT TGAGCAACTT 
AGCCGAATGT TCAAGCCTGA TATCAAAGCG ATCAAGAAGC GTATGGAGGA TTTAATAACC 
AGAGATTATT TGGAGAGGGA CAAGGAGAAT CCTAACATGT TTAGGTACTT GGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CUL1 ( NM_001036498.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001031575.1 Arabidopsis thaliana cullin 1 (CUL1), mRNA.

Price & Availability↑

CloneID	OAb14247
Clone ID Related Accession (Same CDS sequence)	NM_001036499.1 , NM_001036498.3 , NM_001203732.1 , NM_116491.3
Accession Version	NM_001036498.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	cullin 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_001036498.2.

ATGGAGCGCA AGACTATTGA CTTGGAGCAA GGATGGGACT ATATGCAGAC TGGAATTACT 
AAGCTGAAAC GGATTCTGGA AGGATTGAAT GAGCCAGCAT TTGACTCTGA GCAATATATG 
ATGCTTTATA CGACTATCTA CAACATGTGC ACCCAGAAAC CTCCTCATGA TTACTCACAG 
CAGCTTTATG ACAAGTATCG GGAAGCATTT GAGGAATATA TTAACTCAAC AGTTTTGCCT 
GCGTTGAGGG AGAAGCATGA TGAGTTTATG CTGCGGGAGC TATTTAAAAG ATGGTCAAAC 
CATAAAGTAA TGGTCAGATG GCTATCCCGC TTCTTCTACT ACCTTGACCG TTACTTCATT 
GCTCGGAGAT CACTTCCACC ACTGAATGAA GTTGGCCTGA CATGCTTCCG TGACCTGGTT 
TATAATGAGC TACATTCTAA GGTCAAACAA GCTGTAATAG CTCTTGTTGA TAAAGAACGG 
GAGGGCGAGC AGATTGATAG GGCCCTGCTG AAAAACGTAT TAGATATCTA TGTAGAGATT 
GGAATGGGGC AGATGGAGAG GTATGAAGAA GATTTTGAAA GCTTCATGCT TCAAGATACT 
TCTTCGTATT ATTCTCGCAA GGCATCAAGC TGGATTCAGG AAGATTCTTG CCCTGATTAC 
ATGTTGAAGT CTGAAGAATG TCTAAAGAAG GAGAGGGAGA GAGTGGCTCA CTACCTACAC 
TCAAGCAGTG AGCCAAAGCT GGTTGAGAAA GTACAACATG AATTGCTGGT TGTGTTTGCA 
AGTCAGCTTC TAGAAAAAGA ACACTCAGGG TGCCGTGCAT TGCTAAGAGA TGACAAGGTG 
GATGATCTCT CCAGGATGTA CAGGCTTTAC CATAAAATTT TGCGAGGCTT GGAACCTGTT 
GCAAACATCT TTAAGCAGCA TGTCACAGCA GAGGGTAACG CTCTTGTCCA ACAGGCCGAA 
GACACGGCTA CTAATCAGGT TGCAAATACT GCTAGCGTCC AGGAACAGGT TCTTATCAGA 
AAAGTGATTG AACTTCATGA TAAATACATG GTATATGTCA CCGAGTGTTT CCAGAACCAC 
ACCCTCTTCC ATAAGGCTTT GAAAGAGGCA TTTGAGATTT TTTGTAACAA AACGGTTGCT 
GGAAGTTCAA GTGCAGAACT ACTTGCAACA TTTTGCGACA ATATTCTCAA AAAGGGGGGA 
AGTGAAAAGC TGAGTGATGA AGCTATCGAA GATACGCTTG AGAAGGTTGT CAAATTGCTT 
GCATACATAA GTGACAAGGA TCTTTTCGCT GAGTTCTACA GGAAGAAGCT GGCCCGTAGG 
CTCTTATTTG ATCGCAGTGC TAATGATGAT CATGAGAGAA GTATCCTGAC AAAGCTCAAG 
CAACAATGTG GTGGACAGTT TACTTCTAAG ATGGAGGGCA TGGTGACGGA TTTGACACTG 
GCAAGAGAAA ACCAAAACAG TTTCGAGGAT TATCTAGGCA GTAACCCTGC TGCAAACCCA 
GGGATTGACT TGACCGTCAC TGTTCTTACC ACTGGTTTCT GGCCAAGTTA CAAATCATTT 
GACATAAATC TACCCAGTGA AATGATCAAG TGTGTTGAAG TCTTCAAAGG GTTTTATGAA 
ACGAAAACGA AACACAGGAA GCTTACGTGG ATCTATTCAC TGGGAACTTG TCACATAAAC 
GGGAAGTTTG ATCAAAAGGC CATCGAGTTA ATAGTGTCTA CTTACCAGGC TGCTGTGCTT 
CTACTCTTTA ACACAACTGA CAAGTTAAGT TACACTGAGA TCTTGGCTCA ACTGAACCTA 
AGCCATGAAG ATCTAGTTAG GTTGCTTCAT TCCTTGTCAT GTGCTAAGTA CAAGATACTC 
CTTAAGGAGC CAAACACCAA GACTGTCTCC CAGAATGATG CCTTTGAGTT CAACTCCAAA 
TTCACCGATA GAATGCGCAG AATCAAGATC CCTCTTCCCC CAGTTGATGA AAGGAAGAAA 
GTCGTTGAAG ATGTCGATAA AGACAGAAGA TATGCAATTG ATGCTGCCAT TGTCAGGATC 
ATGAAGAGCA GGAAAGTATT GGGACATCAA CAACTTGTTT CTGAGTGTGT TGAGCAACTT 
AGCCGAATGT TCAAGCCTGA TATCAAAGCG ATCAAGAAGC GTATGGAGGA TTTAATAACC 
AGAGATTATT TGGAGAGGGA CAAGGAGAAT CCTAACATGT TTAGGTACTT GGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001031575.1
CDS	240..2456
Translation	MERKTIDLEQGWDYMQTGITKLKRILEGLNEPAFDSEQYMMLYTTIYNMCTQKPPHDYSQQLYDKYREAFEEYINSTVLPALREKHDEFMLRELFKRWSNHKVMVRWLSRFFYYLDRYFIARRSLPPLNEVGLTCFRDLVYNELHSKVKQAVIALVDKEREGEQIDRALLKNVLDIYVEIGMGQMERYEEDFESFMLQDTSSYYSRKASSWIQEDSCPDYMLKSEECLKKERERVAHYLHSSSEPKLVEKVQHELLVVFASQLLEKEHSGCRALLRDDKVDDLSRMYRLYHKILRGLEPVANIFKQHVTAEGNALVQQAEDTATNQVANTASVQEQVLIRKVIELHDKYMVYVTECFQNHTLFHKALKEAFEIFCNKTVAGSSSAELLATFCDNILKKGGSEKLSDEAIEDTLEKVVKLLAYISDKDLFAEFYRKKLARRLLFDRSANDDHERSILTKLKQQCGGQFTSKMEGMVTDLTLARENQNSFEDYLGSNPAANPGIDLTVTVLTTGFWPSYKSFDINLPSEMIKCVEVFKGFYETKTKHRKLTWIYSLGTCHINGKFDQKAIELIVSTYQAAVLLLFNTTDKLSYTEILAQLNLSHEDLVRLLHSLSCAKYKILLKEPNTKTVSQNDAFEFNSKFTDRMRRIKIPLPPVDERKKVVEDVDKDRRYAIDAAIVRIMKSRKVLGHQQLVSECVEQLSRMFKPDIKAIKKRMEDLITRDYLERDKENPNMFRYLA

Target ORF information:

RefSeq Version	NM_001036498.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana cullin 1 (CUL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001036498.3

ORF Insert Sequence:

ATGGAGCGCA AGACTATTGA CTTGGAGCAA GGATGGGACT ATATGCAGAC TGGAATTACT 
AAGCTGAAAC GGATTCTGGA AGGATTGAAT GAGCCAGCAT TTGACTCTGA GCAATATATG 
ATGCTTTATA CGACTATCTA CAACATGTGC ACCCAGAAAC CTCCTCATGA TTACTCACAG 
CAGCTTTATG ACAAGTATCG GGAAGCATTT GAGGAATATA TTAACTCAAC AGTTTTGCCT 
GCGTTGAGGG AGAAGCATGA TGAGTTTATG CTGCGGGAGC TATTTAAAAG ATGGTCAAAC 
CATAAAGTAA TGGTCAGATG GCTATCCCGC TTCTTCTACT ACCTTGACCG TTACTTCATT 
GCTCGGAGAT CACTTCCACC ACTGAATGAA GTTGGCCTGA CATGCTTCCG TGACCTGGTT 
TATAATGAGC TACATTCTAA GGTCAAACAA GCTGTAATAG CTCTTGTTGA TAAAGAACGG 
GAGGGCGAGC AGATTGATAG GGCCCTGCTG AAAAACGTAT TAGATATCTA TGTAGAGATT 
GGAATGGGGC AGATGGAGAG GTATGAAGAA GATTTTGAAA GCTTCATGCT TCAAGATACT 
TCTTCGTATT ATTCTCGCAA GGCATCAAGC TGGATTCAGG AAGATTCTTG CCCTGATTAC 
ATGTTGAAGT CTGAAGAATG TCTAAAGAAG GAGAGGGAGA GAGTGGCTCA CTACCTACAC 
TCAAGCAGTG AGCCAAAGCT GGTTGAGAAA GTACAACATG AATTGCTGGT TGTGTTTGCA 
AGTCAGCTTC TAGAAAAAGA ACACTCAGGG TGCCGTGCAT TGCTAAGAGA TGACAAGGTG 
GATGATCTCT CCAGGATGTA CAGGCTTTAC CATAAAATTT TGCGAGGCTT GGAACCTGTT 
GCAAACATCT TTAAGCAGCA TGTCACAGCA GAGGGTAACG CTCTTGTCCA ACAGGCCGAA 
GACACGGCTA CTAATCAGGT TGCAAATACT GCTAGCGTCC AGGAACAGGT TCTTATCAGA 
AAAGTGATTG AACTTCATGA TAAATACATG GTATATGTCA CCGAGTGTTT CCAGAACCAC 
ACCCTCTTCC ATAAGGCTTT GAAAGAGGCA TTTGAGATTT TTTGTAACAA AACGGTTGCT 
GGAAGTTCAA GTGCAGAACT ACTTGCAACA TTTTGCGACA ATATTCTCAA AAAGGGGGGA 
AGTGAAAAGC TGAGTGATGA AGCTATCGAA GATACGCTTG AGAAGGTTGT CAAATTGCTT 
GCATACATAA GTGACAAGGA TCTTTTCGCT GAGTTCTACA GGAAGAAGCT GGCCCGTAGG 
CTCTTATTTG ATCGCAGTGC TAATGATGAT CATGAGAGAA GTATCCTGAC AAAGCTCAAG 
CAACAATGTG GTGGACAGTT TACTTCTAAG ATGGAGGGCA TGGTGACGGA TTTGACACTG 
GCAAGAGAAA ACCAAAACAG TTTCGAGGAT TATCTAGGCA GTAACCCTGC TGCAAACCCA 
GGGATTGACT TGACCGTCAC TGTTCTTACC ACTGGTTTCT GGCCAAGTTA CAAATCATTT 
GACATAAATC TACCCAGTGA AATGATCAAG TGTGTTGAAG TCTTCAAAGG GTTTTATGAA 
ACGAAAACGA AACACAGGAA GCTTACGTGG ATCTATTCAC TGGGAACTTG TCACATAAAC 
GGGAAGTTTG ATCAAAAGGC CATCGAGTTA ATAGTGTCTA CTTACCAGGC TGCTGTGCTT 
CTACTCTTTA ACACAACTGA CAAGTTAAGT TACACTGAGA TCTTGGCTCA ACTGAACCTA 
AGCCATGAAG ATCTAGTTAG GTTGCTTCAT TCCTTGTCAT GTGCTAAGTA CAAGATACTC 
CTTAAGGAGC CAAACACCAA GACTGTCTCC CAGAATGATG CCTTTGAGTT CAACTCCAAA 
TTCACCGATA GAATGCGCAG AATCAAGATC CCTCTTCCCC CAGTTGATGA AAGGAAGAAA 
GTCGTTGAAG ATGTCGATAA AGACAGAAGA TATGCAATTG ATGCTGCCAT TGTCAGGATC 
ATGAAGAGCA GGAAAGTATT GGGACATCAA CAACTTGTTT CTGAGTGTGT TGAGCAACTT 
AGCCGAATGT TCAAGCCTGA TATCAAAGCG ATCAAGAAGC GTATGGAGGA TTTAATAACC 
AGAGATTATT TGGAGAGGGA CAAGGAGAAT CCTAACATGT TTAGGTACTT GGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CUL1 ( NM_001203732.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001190661.1 Arabidopsis thaliana cullin 1 (CUL1), mRNA.

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CloneID	OAb14247
Clone ID Related Accession (Same CDS sequence)	NM_001036499.1 , NM_001036498.3 , NM_001203732.1 , NM_116491.3
Accession Version	NM_001203732.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	cullin 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGGAGCGCA AGACTATTGA CTTGGAGCAA GGATGGGACT ATATGCAGAC TGGAATTACT 
AAGCTGAAAC GGATTCTGGA AGGATTGAAT GAGCCAGCAT TTGACTCTGA GCAATATATG 
ATGCTTTATA CGACTATCTA CAACATGTGC ACCCAGAAAC CTCCTCATGA TTACTCACAG 
CAGCTTTATG ACAAGTATCG GGAAGCATTT GAGGAATATA TTAACTCAAC AGTTTTGCCT 
GCGTTGAGGG AGAAGCATGA TGAGTTTATG CTGCGGGAGC TATTTAAAAG ATGGTCAAAC 
CATAAAGTAA TGGTCAGATG GCTATCCCGC TTCTTCTACT ACCTTGACCG TTACTTCATT 
GCTCGGAGAT CACTTCCACC ACTGAATGAA GTTGGCCTGA CATGCTTCCG TGACCTGGTT 
TATAATGAGC TACATTCTAA GGTCAAACAA GCTGTAATAG CTCTTGTTGA TAAAGAACGG 
GAGGGCGAGC AGATTGATAG GGCCCTGCTG AAAAACGTAT TAGATATCTA TGTAGAGATT 
GGAATGGGGC AGATGGAGAG GTATGAAGAA GATTTTGAAA GCTTCATGCT TCAAGATACT 
TCTTCGTATT ATTCTCGCAA GGCATCAAGC TGGATTCAGG AAGATTCTTG CCCTGATTAC 
ATGTTGAAGT CTGAAGAATG TCTAAAGAAG GAGAGGGAGA GAGTGGCTCA CTACCTACAC 
TCAAGCAGTG AGCCAAAGCT GGTTGAGAAA GTACAACATG AATTGCTGGT TGTGTTTGCA 
AGTCAGCTTC TAGAAAAAGA ACACTCAGGG TGCCGTGCAT TGCTAAGAGA TGACAAGGTG 
GATGATCTCT CCAGGATGTA CAGGCTTTAC CATAAAATTT TGCGAGGCTT GGAACCTGTT 
GCAAACATCT TTAAGCAGCA TGTCACAGCA GAGGGTAACG CTCTTGTCCA ACAGGCCGAA 
GACACGGCTA CTAATCAGGT TGCAAATACT GCTAGCGTCC AGGAACAGGT TCTTATCAGA 
AAAGTGATTG AACTTCATGA TAAATACATG GTATATGTCA CCGAGTGTTT CCAGAACCAC 
ACCCTCTTCC ATAAGGCTTT GAAAGAGGCA TTTGAGATTT TTTGTAACAA AACGGTTGCT 
GGAAGTTCAA GTGCAGAACT ACTTGCAACA TTTTGCGACA ATATTCTCAA AAAGGGGGGA 
AGTGAAAAGC TGAGTGATGA AGCTATCGAA GATACGCTTG AGAAGGTTGT CAAATTGCTT 
GCATACATAA GTGACAAGGA TCTTTTCGCT GAGTTCTACA GGAAGAAGCT GGCCCGTAGG 
CTCTTATTTG ATCGCAGTGC TAATGATGAT CATGAGAGAA GTATCCTGAC AAAGCTCAAG 
CAACAATGTG GTGGACAGTT TACTTCTAAG ATGGAGGGCA TGGTGACGGA TTTGACACTG 
GCAAGAGAAA ACCAAAACAG TTTCGAGGAT TATCTAGGCA GTAACCCTGC TGCAAACCCA 
GGGATTGACT TGACCGTCAC TGTTCTTACC ACTGGTTTCT GGCCAAGTTA CAAATCATTT 
GACATAAATC TACCCAGTGA AATGATCAAG TGTGTTGAAG TCTTCAAAGG GTTTTATGAA 
ACGAAAACGA AACACAGGAA GCTTACGTGG ATCTATTCAC TGGGAACTTG TCACATAAAC 
GGGAAGTTTG ATCAAAAGGC CATCGAGTTA ATAGTGTCTA CTTACCAGGC TGCTGTGCTT 
CTACTCTTTA ACACAACTGA CAAGTTAAGT TACACTGAGA TCTTGGCTCA ACTGAACCTA 
AGCCATGAAG ATCTAGTTAG GTTGCTTCAT TCCTTGTCAT GTGCTAAGTA CAAGATACTC 
CTTAAGGAGC CAAACACCAA GACTGTCTCC CAGAATGATG CCTTTGAGTT CAACTCCAAA 
TTCACCGATA GAATGCGCAG AATCAAGATC CCTCTTCCCC CAGTTGATGA AAGGAAGAAA 
GTCGTTGAAG ATGTCGATAA AGACAGAAGA TATGCAATTG ATGCTGCCAT TGTCAGGATC 
ATGAAGAGCA GGAAAGTATT GGGACATCAA CAACTTGTTT CTGAGTGTGT TGAGCAACTT 
AGCCGAATGT TCAAGCCTGA TATCAAAGCG ATCAAGAAGC GTATGGAGGA TTTAATAACC 
AGAGATTATT TGGAGAGGGA CAAGGAGAAT CCTAACATGT TTAGGTACTT GGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001190661.1
CDS	238..2454
Translation	MERKTIDLEQGWDYMQTGITKLKRILEGLNEPAFDSEQYMMLYTTIYNMCTQKPPHDYSQQLYDKYREAFEEYINSTVLPALREKHDEFMLRELFKRWSNHKVMVRWLSRFFYYLDRYFIARRSLPPLNEVGLTCFRDLVYNELHSKVKQAVIALVDKEREGEQIDRALLKNVLDIYVEIGMGQMERYEEDFESFMLQDTSSYYSRKASSWIQEDSCPDYMLKSEECLKKERERVAHYLHSSSEPKLVEKVQHELLVVFASQLLEKEHSGCRALLRDDKVDDLSRMYRLYHKILRGLEPVANIFKQHVTAEGNALVQQAEDTATNQVANTASVQEQVLIRKVIELHDKYMVYVTECFQNHTLFHKALKEAFEIFCNKTVAGSSSAELLATFCDNILKKGGSEKLSDEAIEDTLEKVVKLLAYISDKDLFAEFYRKKLARRLLFDRSANDDHERSILTKLKQQCGGQFTSKMEGMVTDLTLARENQNSFEDYLGSNPAANPGIDLTVTVLTTGFWPSYKSFDINLPSEMIKCVEVFKGFYETKTKHRKLTWIYSLGTCHINGKFDQKAIELIVSTYQAAVLLLFNTTDKLSYTEILAQLNLSHEDLVRLLHSLSCAKYKILLKEPNTKTVSQNDAFEFNSKFTDRMRRIKIPLPPVDERKKVVEDVDKDRRYAIDAAIVRIMKSRKVLGHQQLVSECVEQLSRMFKPDIKAIKKRMEDLITRDYLERDKENPNMFRYLA

Target ORF information:

RefSeq Version	NM_001203732.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana cullin 1 (CUL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001203732.1

ORF Insert Sequence:

ATGGAGCGCA AGACTATTGA CTTGGAGCAA GGATGGGACT ATATGCAGAC TGGAATTACT 
AAGCTGAAAC GGATTCTGGA AGGATTGAAT GAGCCAGCAT TTGACTCTGA GCAATATATG 
ATGCTTTATA CGACTATCTA CAACATGTGC ACCCAGAAAC CTCCTCATGA TTACTCACAG 
CAGCTTTATG ACAAGTATCG GGAAGCATTT GAGGAATATA TTAACTCAAC AGTTTTGCCT 
GCGTTGAGGG AGAAGCATGA TGAGTTTATG CTGCGGGAGC TATTTAAAAG ATGGTCAAAC 
CATAAAGTAA TGGTCAGATG GCTATCCCGC TTCTTCTACT ACCTTGACCG TTACTTCATT 
GCTCGGAGAT CACTTCCACC ACTGAATGAA GTTGGCCTGA CATGCTTCCG TGACCTGGTT 
TATAATGAGC TACATTCTAA GGTCAAACAA GCTGTAATAG CTCTTGTTGA TAAAGAACGG 
GAGGGCGAGC AGATTGATAG GGCCCTGCTG AAAAACGTAT TAGATATCTA TGTAGAGATT 
GGAATGGGGC AGATGGAGAG GTATGAAGAA GATTTTGAAA GCTTCATGCT TCAAGATACT 
TCTTCGTATT ATTCTCGCAA GGCATCAAGC TGGATTCAGG AAGATTCTTG CCCTGATTAC 
ATGTTGAAGT CTGAAGAATG TCTAAAGAAG GAGAGGGAGA GAGTGGCTCA CTACCTACAC 
TCAAGCAGTG AGCCAAAGCT GGTTGAGAAA GTACAACATG AATTGCTGGT TGTGTTTGCA 
AGTCAGCTTC TAGAAAAAGA ACACTCAGGG TGCCGTGCAT TGCTAAGAGA TGACAAGGTG 
GATGATCTCT CCAGGATGTA CAGGCTTTAC CATAAAATTT TGCGAGGCTT GGAACCTGTT 
GCAAACATCT TTAAGCAGCA TGTCACAGCA GAGGGTAACG CTCTTGTCCA ACAGGCCGAA 
GACACGGCTA CTAATCAGGT TGCAAATACT GCTAGCGTCC AGGAACAGGT TCTTATCAGA 
AAAGTGATTG AACTTCATGA TAAATACATG GTATATGTCA CCGAGTGTTT CCAGAACCAC 
ACCCTCTTCC ATAAGGCTTT GAAAGAGGCA TTTGAGATTT TTTGTAACAA AACGGTTGCT 
GGAAGTTCAA GTGCAGAACT ACTTGCAACA TTTTGCGACA ATATTCTCAA AAAGGGGGGA 
AGTGAAAAGC TGAGTGATGA AGCTATCGAA GATACGCTTG AGAAGGTTGT CAAATTGCTT 
GCATACATAA GTGACAAGGA TCTTTTCGCT GAGTTCTACA GGAAGAAGCT GGCCCGTAGG 
CTCTTATTTG ATCGCAGTGC TAATGATGAT CATGAGAGAA GTATCCTGAC AAAGCTCAAG 
CAACAATGTG GTGGACAGTT TACTTCTAAG ATGGAGGGCA TGGTGACGGA TTTGACACTG 
GCAAGAGAAA ACCAAAACAG TTTCGAGGAT TATCTAGGCA GTAACCCTGC TGCAAACCCA 
GGGATTGACT TGACCGTCAC TGTTCTTACC ACTGGTTTCT GGCCAAGTTA CAAATCATTT 
GACATAAATC TACCCAGTGA AATGATCAAG TGTGTTGAAG TCTTCAAAGG GTTTTATGAA 
ACGAAAACGA AACACAGGAA GCTTACGTGG ATCTATTCAC TGGGAACTTG TCACATAAAC 
GGGAAGTTTG ATCAAAAGGC CATCGAGTTA ATAGTGTCTA CTTACCAGGC TGCTGTGCTT 
CTACTCTTTA ACACAACTGA CAAGTTAAGT TACACTGAGA TCTTGGCTCA ACTGAACCTA 
AGCCATGAAG ATCTAGTTAG GTTGCTTCAT TCCTTGTCAT GTGCTAAGTA CAAGATACTC 
CTTAAGGAGC CAAACACCAA GACTGTCTCC CAGAATGATG CCTTTGAGTT CAACTCCAAA 
TTCACCGATA GAATGCGCAG AATCAAGATC CCTCTTCCCC CAGTTGATGA AAGGAAGAAA 
GTCGTTGAAG ATGTCGATAA AGACAGAAGA TATGCAATTG ATGCTGCCAT TGTCAGGATC 
ATGAAGAGCA GGAAAGTATT GGGACATCAA CAACTTGTTT CTGAGTGTGT TGAGCAACTT 
AGCCGAATGT TCAAGCCTGA TATCAAAGCG ATCAAGAAGC GTATGGAGGA TTTAATAACC 
AGAGATTATT TGGAGAGGGA CAAGGAGAAT CCTAACATGT TTAGGTACTT GGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CUL1 ( NM_116491.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_567243.1 Arabidopsis thaliana cullin 1 (CUL1), mRNA.

Price & Availability↑

CloneID	OAb14247
Clone ID Related Accession (Same CDS sequence)	NM_001036499.1 , NM_001036498.3 , NM_001203732.1 , NM_116491.3
Accession Version	NM_116491.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	cullin 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_116491.2.

ATGGAGCGCA AGACTATTGA CTTGGAGCAA GGATGGGACT ATATGCAGAC TGGAATTACT 
AAGCTGAAAC GGATTCTGGA AGGATTGAAT GAGCCAGCAT TTGACTCTGA GCAATATATG 
ATGCTTTATA CGACTATCTA CAACATGTGC ACCCAGAAAC CTCCTCATGA TTACTCACAG 
CAGCTTTATG ACAAGTATCG GGAAGCATTT GAGGAATATA TTAACTCAAC AGTTTTGCCT 
GCGTTGAGGG AGAAGCATGA TGAGTTTATG CTGCGGGAGC TATTTAAAAG ATGGTCAAAC 
CATAAAGTAA TGGTCAGATG GCTATCCCGC TTCTTCTACT ACCTTGACCG TTACTTCATT 
GCTCGGAGAT CACTTCCACC ACTGAATGAA GTTGGCCTGA CATGCTTCCG TGACCTGGTT 
TATAATGAGC TACATTCTAA GGTCAAACAA GCTGTAATAG CTCTTGTTGA TAAAGAACGG 
GAGGGCGAGC AGATTGATAG GGCCCTGCTG AAAAACGTAT TAGATATCTA TGTAGAGATT 
GGAATGGGGC AGATGGAGAG GTATGAAGAA GATTTTGAAA GCTTCATGCT TCAAGATACT 
TCTTCGTATT ATTCTCGCAA GGCATCAAGC TGGATTCAGG AAGATTCTTG CCCTGATTAC 
ATGTTGAAGT CTGAAGAATG TCTAAAGAAG GAGAGGGAGA GAGTGGCTCA CTACCTACAC 
TCAAGCAGTG AGCCAAAGCT GGTTGAGAAA GTACAACATG AATTGCTGGT TGTGTTTGCA 
AGTCAGCTTC TAGAAAAAGA ACACTCAGGG TGCCGTGCAT TGCTAAGAGA TGACAAGGTG 
GATGATCTCT CCAGGATGTA CAGGCTTTAC CATAAAATTT TGCGAGGCTT GGAACCTGTT 
GCAAACATCT TTAAGCAGCA TGTCACAGCA GAGGGTAACG CTCTTGTCCA ACAGGCCGAA 
GACACGGCTA CTAATCAGGT TGCAAATACT GCTAGCGTCC AGGAACAGGT TCTTATCAGA 
AAAGTGATTG AACTTCATGA TAAATACATG GTATATGTCA CCGAGTGTTT CCAGAACCAC 
ACCCTCTTCC ATAAGGCTTT GAAAGAGGCA TTTGAGATTT TTTGTAACAA AACGGTTGCT 
GGAAGTTCAA GTGCAGAACT ACTTGCAACA TTTTGCGACA ATATTCTCAA AAAGGGGGGA 
AGTGAAAAGC TGAGTGATGA AGCTATCGAA GATACGCTTG AGAAGGTTGT CAAATTGCTT 
GCATACATAA GTGACAAGGA TCTTTTCGCT GAGTTCTACA GGAAGAAGCT GGCCCGTAGG 
CTCTTATTTG ATCGCAGTGC TAATGATGAT CATGAGAGAA GTATCCTGAC AAAGCTCAAG 
CAACAATGTG GTGGACAGTT TACTTCTAAG ATGGAGGGCA TGGTGACGGA TTTGACACTG 
GCAAGAGAAA ACCAAAACAG TTTCGAGGAT TATCTAGGCA GTAACCCTGC TGCAAACCCA 
GGGATTGACT TGACCGTCAC TGTTCTTACC ACTGGTTTCT GGCCAAGTTA CAAATCATTT 
GACATAAATC TACCCAGTGA AATGATCAAG TGTGTTGAAG TCTTCAAAGG GTTTTATGAA 
ACGAAAACGA AACACAGGAA GCTTACGTGG ATCTATTCAC TGGGAACTTG TCACATAAAC 
GGGAAGTTTG ATCAAAAGGC CATCGAGTTA ATAGTGTCTA CTTACCAGGC TGCTGTGCTT 
CTACTCTTTA ACACAACTGA CAAGTTAAGT TACACTGAGA TCTTGGCTCA ACTGAACCTA 
AGCCATGAAG ATCTAGTTAG GTTGCTTCAT TCCTTGTCAT GTGCTAAGTA CAAGATACTC 
CTTAAGGAGC CAAACACCAA GACTGTCTCC CAGAATGATG CCTTTGAGTT CAACTCCAAA 
TTCACCGATA GAATGCGCAG AATCAAGATC CCTCTTCCCC CAGTTGATGA AAGGAAGAAA 
GTCGTTGAAG ATGTCGATAA AGACAGAAGA TATGCAATTG ATGCTGCCAT TGTCAGGATC 
ATGAAGAGCA GGAAAGTATT GGGACATCAA CAACTTGTTT CTGAGTGTGT TGAGCAACTT 
AGCCGAATGT TCAAGCCTGA TATCAAAGCG ATCAAGAAGC GTATGGAGGA TTTAATAACC 
AGAGATTATT TGGAGAGGGA CAAGGAGAAT CCTAACATGT TTAGGTACTT GGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_567243.1
CDS	334..2550
Translation	MERKTIDLEQGWDYMQTGITKLKRILEGLNEPAFDSEQYMMLYTTIYNMCTQKPPHDYSQQLYDKYREAFEEYINSTVLPALREKHDEFMLRELFKRWSNHKVMVRWLSRFFYYLDRYFIARRSLPPLNEVGLTCFRDLVYNELHSKVKQAVIALVDKEREGEQIDRALLKNVLDIYVEIGMGQMERYEEDFESFMLQDTSSYYSRKASSWIQEDSCPDYMLKSEECLKKERERVAHYLHSSSEPKLVEKVQHELLVVFASQLLEKEHSGCRALLRDDKVDDLSRMYRLYHKILRGLEPVANIFKQHVTAEGNALVQQAEDTATNQVANTASVQEQVLIRKVIELHDKYMVYVTECFQNHTLFHKALKEAFEIFCNKTVAGSSSAELLATFCDNILKKGGSEKLSDEAIEDTLEKVVKLLAYISDKDLFAEFYRKKLARRLLFDRSANDDHERSILTKLKQQCGGQFTSKMEGMVTDLTLARENQNSFEDYLGSNPAANPGIDLTVTVLTTGFWPSYKSFDINLPSEMIKCVEVFKGFYETKTKHRKLTWIYSLGTCHINGKFDQKAIELIVSTYQAAVLLLFNTTDKLSYTEILAQLNLSHEDLVRLLHSLSCAKYKILLKEPNTKTVSQNDAFEFNSKFTDRMRRIKIPLPPVDERKKVVEDVDKDRRYAIDAAIVRIMKSRKVLGHQQLVSECVEQLSRMFKPDIKAIKKRMEDLITRDYLERDKENPNMFRYLA

Target ORF information:

RefSeq Version	NM_116491.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana cullin 1 (CUL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_116491.3

ORF Insert Sequence:

ATGGAGCGCA AGACTATTGA CTTGGAGCAA GGATGGGACT ATATGCAGAC TGGAATTACT 
AAGCTGAAAC GGATTCTGGA AGGATTGAAT GAGCCAGCAT TTGACTCTGA GCAATATATG 
ATGCTTTATA CGACTATCTA CAACATGTGC ACCCAGAAAC CTCCTCATGA TTACTCACAG 
CAGCTTTATG ACAAGTATCG GGAAGCATTT GAGGAATATA TTAACTCAAC AGTTTTGCCT 
GCGTTGAGGG AGAAGCATGA TGAGTTTATG CTGCGGGAGC TATTTAAAAG ATGGTCAAAC 
CATAAAGTAA TGGTCAGATG GCTATCCCGC TTCTTCTACT ACCTTGACCG TTACTTCATT 
GCTCGGAGAT CACTTCCACC ACTGAATGAA GTTGGCCTGA CATGCTTCCG TGACCTGGTT 
TATAATGAGC TACATTCTAA GGTCAAACAA GCTGTAATAG CTCTTGTTGA TAAAGAACGG 
GAGGGCGAGC AGATTGATAG GGCCCTGCTG AAAAACGTAT TAGATATCTA TGTAGAGATT 
GGAATGGGGC AGATGGAGAG GTATGAAGAA GATTTTGAAA GCTTCATGCT TCAAGATACT 
TCTTCGTATT ATTCTCGCAA GGCATCAAGC TGGATTCAGG AAGATTCTTG CCCTGATTAC 
ATGTTGAAGT CTGAAGAATG TCTAAAGAAG GAGAGGGAGA GAGTGGCTCA CTACCTACAC 
TCAAGCAGTG AGCCAAAGCT GGTTGAGAAA GTACAACATG AATTGCTGGT TGTGTTTGCA 
AGTCAGCTTC TAGAAAAAGA ACACTCAGGG TGCCGTGCAT TGCTAAGAGA TGACAAGGTG 
GATGATCTCT CCAGGATGTA CAGGCTTTAC CATAAAATTT TGCGAGGCTT GGAACCTGTT 
GCAAACATCT TTAAGCAGCA TGTCACAGCA GAGGGTAACG CTCTTGTCCA ACAGGCCGAA 
GACACGGCTA CTAATCAGGT TGCAAATACT GCTAGCGTCC AGGAACAGGT TCTTATCAGA 
AAAGTGATTG AACTTCATGA TAAATACATG GTATATGTCA CCGAGTGTTT CCAGAACCAC 
ACCCTCTTCC ATAAGGCTTT GAAAGAGGCA TTTGAGATTT TTTGTAACAA AACGGTTGCT 
GGAAGTTCAA GTGCAGAACT ACTTGCAACA TTTTGCGACA ATATTCTCAA AAAGGGGGGA 
AGTGAAAAGC TGAGTGATGA AGCTATCGAA GATACGCTTG AGAAGGTTGT CAAATTGCTT 
GCATACATAA GTGACAAGGA TCTTTTCGCT GAGTTCTACA GGAAGAAGCT GGCCCGTAGG 
CTCTTATTTG ATCGCAGTGC TAATGATGAT CATGAGAGAA GTATCCTGAC AAAGCTCAAG 
CAACAATGTG GTGGACAGTT TACTTCTAAG ATGGAGGGCA TGGTGACGGA TTTGACACTG 
GCAAGAGAAA ACCAAAACAG TTTCGAGGAT TATCTAGGCA GTAACCCTGC TGCAAACCCA 
GGGATTGACT TGACCGTCAC TGTTCTTACC ACTGGTTTCT GGCCAAGTTA CAAATCATTT 
GACATAAATC TACCCAGTGA AATGATCAAG TGTGTTGAAG TCTTCAAAGG GTTTTATGAA 
ACGAAAACGA AACACAGGAA GCTTACGTGG ATCTATTCAC TGGGAACTTG TCACATAAAC 
GGGAAGTTTG ATCAAAAGGC CATCGAGTTA ATAGTGTCTA CTTACCAGGC TGCTGTGCTT 
CTACTCTTTA ACACAACTGA CAAGTTAAGT TACACTGAGA TCTTGGCTCA ACTGAACCTA 
AGCCATGAAG ATCTAGTTAG GTTGCTTCAT TCCTTGTCAT GTGCTAAGTA CAAGATACTC 
CTTAAGGAGC CAAACACCAA GACTGTCTCC CAGAATGATG CCTTTGAGTT CAACTCCAAA 
TTCACCGATA GAATGCGCAG AATCAAGATC CCTCTTCCCC CAGTTGATGA AAGGAAGAAA 
GTCGTTGAAG ATGTCGATAA AGACAGAAGA TATGCAATTG ATGCTGCCAT TGTCAGGATC 
ATGAAGAGCA GGAAAGTATT GGGACATCAA CAACTTGTTT CTGAGTGTGT TGAGCAACTT 
AGCCGAATGT TCAAGCCTGA TATCAAAGCG ATCAAGAAGC GTATGGAGGA TTTAATAACC 
AGAGATTATT TGGAGAGGGA CAAGGAGAAT CCTAACATGT TTAGGTACTT GGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
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CUL1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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