PAT2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	PAT2
Entrez Gene ID	824714
Full Name	protein affected trafficking 2
Synonyms	AP-3 beta,WAT1,WEAK ACID TOLERANT 1,beta-subunit of adaptor protein complex 3,protein affected trafficking 2
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_202714.4	NP_974443.4	protein affected trafficking 2
NM_115406.5	NP_567022.1	protein affected trafficking 2

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	PAT2	NP_974443.4
Oryza sativa (rice)	Os01g0973300	NP_001045546.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Vacuolar invertases (VIs) might follow an alternative, adaptor protein 3 (AP3)-dependent route in addition to the classical sorting pathway.
Title: Trafficking of plant vacuolar invertases: from a membrane-anchored to a soluble status. Understanding sorting information in their complex N-terminal motifs.

The phenotypes of the wat1-1D mutant can be explained if loss of function of the AP-3 beta-adaptin (PAT2) causes activation of channels or transporters for organic anions and for K(+) at the plasma membrane.
Title: A dominant-negative form of Arabidopsis AP-3 ?-adaptin improves intracellular pH homeostasis.

In Arabidopsis plants defective in the beta-subunit of the AP-3 adaptor complex, INT1 is correctly localized to the tonoplast, while sorting of the vacuolar sucrose transporter SUC4 is blocked in cis-Golgi stacks.
Title: Routes to the tonoplast: the sorting of tonoplast transporters in Arabidopsis mesophyll protoplasts.

Evolutionarily conserved AP-3 beta plays a role in the biogenesis and function of the lytic vacuole.
Title: The AP-3 ? adaptin mediates the biogenesis and function of lytic vacuoles in Arabidopsis.

The following PAT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PAT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb31662	NM_115406.5 Latest version!	Arabidopsis thaliana protein affected trafficking 2 (PAT2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb31661	NM_202714.4 Latest version!	Arabidopsis thaliana protein affected trafficking 2 (PAT2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PAT2 ( NM_115406.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_567022.1 Arabidopsis thaliana protein affected trafficking 2 (PAT2), mRNA.

Price & Availability↑

CloneID	OAb31662
Clone ID Related Accession (Same CDS sequence)	NM_115406.5
Accession Version	NM_115406.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	protein affected trafficking 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Jun 19, 2009 this sequence version replaced NM_115406.4.

ATGGCTGGGA TTCGCTTACA TGTTATTGCA CCTCTTGCAC TTGCAGCTGT GAGTAAATGT 
GCTAGAGATC CAGCTGTTTA TGTTCGGAGA TGTGCTGCAA ACGCTCTTCC GAAGTTGCAT 
GATTTGCGGC TGGAAGAACA TGCTTCTGCG ATTGAAGAGC TAGTTGGGAT ACTGTTGAAT 
GACCATTCTC CAGGAGTAGT TGGAGCTGCG GCAGCGGCAT TCACCTCTAT CTGTCCAAAT 
AACTTCAAAC TGATTGGGAA GAACTATAAG AAGTTGTGCC AGATTCTCCC TGACGTTGAA 
GAGTGGGGTC AGATATTACT CATAGGAACT CTTTTGCGCT ATGTGGTTGC TAGGCATGGA 
CTTGTACGGG AATCTTTGAT GTTGTCCATA CATGGTACGA ATAGTAATGG CTTCTGTGAG 
AAGGATGGCC TAGGCAGAGA TCTTACATTG GATAAAGAAG ATGGTGGTAA AAGTGATTCA 
TTTGATGTTA ATCTGGTCAG TCTTGTATCT AAATGTTACA TTCAAGGTCC AGATGAGTAT 
CTATCAAGAT CCAGTTGCAC CGATACAGTG TCATCTGCTT TCGATACCAA AGAGACCACA 
TCTATTGCAC ATAATGAAGA TGTTAAGATC CTGCTTCAGT GTACATCTCC ATTGTTATGG 
AGTAATAACA GTGCGGTTGT ACTAGCAGCA GCTGGCGTTC AATGGATAAT GGCGCCTCTG 
GAAGATGTTA AGAAAATTGT TAAACCGCTC TTGTTTTTGC TTAGGTCATC TAGCGCCTCC 
AAATATGTGG TCCTCTGCAA TATCCTAGTT TTTGCCAAAG CAGTCCCTTC TCTCTTTGCT 
CCACACTTCG AAAATTTCTT TATTTGTTCC TCAGATGCAT ATCAAGTGAA AGCATACAAG 
CTGGAAATGC TTTCTCTCAT TGCTACCACT TCATCTATCG CTTCAATTTT GAGAGAGTTT 
GAGGATTATA TTAAAGATCC AGACAGGAGA TTTGCGGCTG ATACAGTTGC AGCAATTGGT 
TTGTGCGCAA AAAGACTGAT GACAATTCCA ACTACATGTC TTGATGGATT GTTAGCACTA 
GTTCGACAGG AATCTTTTGC TGGCGATTTT GAGTCCGCGG ATGGAGAAGC TGGAGTATTG 
GTGCAAGCCG TGATGTCAAT TCAGACTATG ATAGAGCGAG ACCCCCTTCG TCATGAGAAA 
GTGTTAATTC AGCTGTTTCG TAGTCTGGAT TCAATCAAGG TAGCTGCTGC TCGTGCAACT 
ATTATTTGGA TGGTGGGGGT CTACTGCTCT TTGGGTCATA TCATTCCTAG GATGCTGACC 
ACAATAACCA AGTACCTTGC ATGGAGCTTT AAATCAGAGG CATCAGAGAC AAAACTACAG 
ATTCTTAATA CTATTGCTAA GGTTCTAATA AGTGCAGAGG CTGGTGACTT TCATATGTTG 
AAAAGGATTG TGGTCTATGT GTTTGAATTG GGCGAATATG ACTTAAGCTA TGATATTCGT 
GATCGAACTC GTTTCCTGAA GAAACTGCTG TCATGCAAAT TAGCTAGTCA TGAGCCGGCA 
GAAGACAGTG TTGCATCACA AGAAAATATT GCTGCGCATG TTGTGGAACA TGTCTTTGGA 
AGAAAACTGA AATCTGTTTC ACCTATAACC CTGCACAATC GTTTTTATCT TCCTGGATCT 
CTGTCACAGA TAGTTCTTCA TGCAGCCCCT GGATATGAAC CTCTTCCAAA GCCCTGCAGT 
TTTGTGTATG AAGAACAGGA CCAGCTGTCA GATTTAGATA AACAAAGAGA GGCCGCAGCT 
GATTTAGATG GTTCTGAGGA ATCCTCTGAA ACAGGGGATG AGAATGGCTC CTCTGATTAT 
GACTCTGAAT CTTCTAATGG TTCGGATTTT AGCAGTGAAG GTGATGAAAG AACAGTGTCT 
AATGACGCCA ATGACCCAGC TGCTCCTTTA ATTCAAATTT CAGAGACTTC TGTTTCCGCT 
GACCAGGAAG AACTAAGGTC CAGAAGGGCC CTGGATTTGT GGTTAGATGA TCAGCCTAGT 
ACATCAAACC AAACTCCATC TGCATTAAAT AGTAATCAGA GCTCTTATGC GAAGATATCC 
ATTGGTGATG TAGGAAGCCG AGTTAAGCCT AAAAGCTATA GTCTCGTAGA CCCTGGAAAT 
GGCAGTGGCT TGAAGGTAGA CTATGCCTTT TTATCTGAAG TCTCTAATGT ATCTCCATTA 
CATGTTTGCG TAGAAGTTCT GTTTGAGAAC AGTTCTGCTG AACCTATCCT GGAGGTGAAT 
TTGGAAGATG AAGAATCAAT GAAGGTAGCA GACTCTTCTG AACAGACATT GGTTGGTAAA 
GCAAATGCAT CTTACAACAA TATACCAACC CTAATTCCAA TGGAGGAAAT TAGCTGCCTT 
GAACCACATC AGAGCACAAA GAGGCTTATC CAGGTTCGGT TTCATCACCA CCTGCTTCCC 
ATGAGGTTAA CCTTACATTA CAATGAAAAG AAAGTTCCTG TCAAGTTACG ACCTGATCTT 
GGATACCTTG TGAAACCATT TTCAATGAGC ATCGAGGAAT TCTTAGCTAC AGAATCTCGA 
TTACCTGGAA TGTTTGAGTA TAGTCGAAGG TGTACTTTTG ACGATCATGT TAAAGATTCA 
AGAACGGAGA ATGGGAAAGA CAAATTTCTT AGCATCTGCG AGAGTATAAC ACTAAAGGTG 
CTTAGCAACT CAAATCTACA CCTTGTATCT GTGGATTTAC CAGTTGCAAA CTCACTCGAA 
GATGCAACTG GTCTGCGATT AAGATTCAGC AGCAAAATCT TGAGCAGTGA GATCCCTCTT 
TTGATTACCA TCACAGTCGA AGGCAAATGC ACCGAAGTTC TGAATTTAAC AGTCAAGATC 
AATTGCGAAG AAACGGTTTT TGGTTTAAAC CTGTTGAACA GGATAGCTAA TTTCATGGTT 
GAGCCGTCTT CTTCAGCCAC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_567022.1
CDS	534..3497
Translation	MAGIRLHVIAPLALAAVSKCARDPAVYVRRCAANALPKLHDLRLEEHASAIEELVGILLNDHSPGVVGAAAAAFTSICPNNFKLIGKNYKKLCQILPDVEEWGQILLIGTLLRYVVARHGLVRESLMLSIHGTNSNGFCEKDGLGRDLTLDKEDGGKSDSFDVNLVSLVSKCYIQGPDEYLSRSSCTDTVSSAFDTKETTSIAHNEDVKILLQCTSPLLWSNNSAVVLAAAGVQWIMAPLEDVKKIVKPLLFLLRSSSASKYVVLCNILVFAKAVPSLFAPHFENFFICSSDAYQVKAYKLEMLSLIATTSSIASILREFEDYIKDPDRRFAADTVAAIGLCAKRLMTIPTTCLDGLLALVRQESFAGDFESADGEAGVLVQAVMSIQTMIERDPLRHEKVLIQLFRSLDSIKVAAARATIIWMVGVYCSLGHIIPRMLTTITKYLAWSFKSEASETKLQILNTIAKVLISAEAGDFHMLKRIVVYVFELGEYDLSYDIRDRTRFLKKLLSCKLASHEPAEDSVASQENIAAHVVEHVFGRKLKSVSPITLHNRFYLPGSLSQIVLHAAPGYEPLPKPCSFVYEEQDQLSDLDKQREAAADLDGSEESSETGDENGSSDYDSESSNGSDFSSEGDERTVSNDANDPAAPLIQISETSVSADQEELRSRRALDLWLDDQPSTSNQTPSALNSNQSSYAKISIGDVGSRVKPKSYSLVDPGNGSGLKVDYAFLSEVSNVSPLHVCVEVLFENSSAEPILEVNLEDEESMKVADSSEQTLVGKANASYNNIPTLIPMEEISCLEPHQSTKRLIQVRFHHHLLPMRLTLHYNEKKVPVKLRPDLGYLVKPFSMSIEEFLATESRLPGMFEYSRRCTFDDHVKDSRTENGKDKFLSICESITLKVLSNSNLHLVSVDLPVANSLEDATGLRLRFSSKILSSEIPLLITITVEGKCTEVLNLTVKINCEETVFGLNLLNRIANFMVEPSSSAT

Target ORF information:

RefSeq Version	NM_115406.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana protein affected trafficking 2 (PAT2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_115406.5

ORF Insert Sequence:

ATGGCTGGGA TTCGCTTACA TGTTATTGCA CCTCTTGCAC TTGCAGCTGT GAGTAAATGT 
GCTAGAGATC CAGCTGTTTA TGTTCGGAGA TGTGCTGCAA ACGCTCTTCC GAAGTTGCAT 
GATTTGCGGC TGGAAGAACA TGCTTCTGCG ATTGAAGAGC TAGTTGGGAT ACTGTTGAAT 
GACCATTCTC CAGGAGTAGT TGGAGCTGCG GCAGCGGCAT TCACCTCTAT CTGTCCAAAT 
AACTTCAAAC TGATTGGGAA GAACTATAAG AAGTTGTGCC AGATTCTCCC TGACGTTGAA 
GAGTGGGGTC AGATATTACT CATAGGAACT CTTTTGCGCT ATGTGGTTGC TAGGCATGGA 
CTTGTACGGG AATCTTTGAT GTTGTCCATA CATGGTACGA ATAGTAATGG CTTCTGTGAG 
AAGGATGGCC TAGGCAGAGA TCTTACATTG GATAAAGAAG ATGGTGGTAA AAGTGATTCA 
TTTGATGTTA ATCTGGTCAG TCTTGTATCT AAATGTTACA TTCAAGGTCC AGATGAGTAT 
CTATCAAGAT CCAGTTGCAC CGATACAGTG TCATCTGCTT TCGATACCAA AGAGACCACA 
TCTATTGCAC ATAATGAAGA TGTTAAGATC CTGCTTCAGT GTACATCTCC ATTGTTATGG 
AGTAATAACA GTGCGGTTGT ACTAGCAGCA GCTGGCGTTC AATGGATAAT GGCGCCTCTG 
GAAGATGTTA AGAAAATTGT TAAACCGCTC TTGTTTTTGC TTAGGTCATC TAGCGCCTCC 
AAATATGTGG TCCTCTGCAA TATCCTAGTT TTTGCCAAAG CAGTCCCTTC TCTCTTTGCT 
CCACACTTCG AAAATTTCTT TATTTGTTCC TCAGATGCAT ATCAAGTGAA AGCATACAAG 
CTGGAAATGC TTTCTCTCAT TGCTACCACT TCATCTATCG CTTCAATTTT GAGAGAGTTT 
GAGGATTATA TTAAAGATCC AGACAGGAGA TTTGCGGCTG ATACAGTTGC AGCAATTGGT 
TTGTGCGCAA AAAGACTGAT GACAATTCCA ACTACATGTC TTGATGGATT GTTAGCACTA 
GTTCGACAGG AATCTTTTGC TGGCGATTTT GAGTCCGCGG ATGGAGAAGC TGGAGTATTG 
GTGCAAGCCG TGATGTCAAT TCAGACTATG ATAGAGCGAG ACCCCCTTCG TCATGAGAAA 
GTGTTAATTC AGCTGTTTCG TAGTCTGGAT TCAATCAAGG TAGCTGCTGC TCGTGCAACT 
ATTATTTGGA TGGTGGGGGT CTACTGCTCT TTGGGTCATA TCATTCCTAG GATGCTGACC 
ACAATAACCA AGTACCTTGC ATGGAGCTTT AAATCAGAGG CATCAGAGAC AAAACTACAG 
ATTCTTAATA CTATTGCTAA GGTTCTAATA AGTGCAGAGG CTGGTGACTT TCATATGTTG 
AAAAGGATTG TGGTCTATGT GTTTGAATTG GGCGAATATG ACTTAAGCTA TGATATTCGT 
GATCGAACTC GTTTCCTGAA GAAACTGCTG TCATGCAAAT TAGCTAGTCA TGAGCCGGCA 
GAAGACAGTG TTGCATCACA AGAAAATATT GCTGCGCATG TTGTGGAACA TGTCTTTGGA 
AGAAAACTGA AATCTGTTTC ACCTATAACC CTGCACAATC GTTTTTATCT TCCTGGATCT 
CTGTCACAGA TAGTTCTTCA TGCAGCCCCT GGATATGAAC CTCTTCCAAA GCCCTGCAGT 
TTTGTGTATG AAGAACAGGA CCAGCTGTCA GATTTAGATA AACAAAGAGA GGCCGCAGCT 
GATTTAGATG GTTCTGAGGA ATCCTCTGAA ACAGGGGATG AGAATGGCTC CTCTGATTAT 
GACTCTGAAT CTTCTAATGG TTCGGATTTT AGCAGTGAAG GTGATGAAAG AACAGTGTCT 
AATGACGCCA ATGACCCAGC TGCTCCTTTA ATTCAAATTT CAGAGACTTC TGTTTCCGCT 
GACCAGGAAG AACTAAGGTC CAGAAGGGCC CTGGATTTGT GGTTAGATGA TCAGCCTAGT 
ACATCAAACC AAACTCCATC TGCATTAAAT AGTAATCAGA GCTCTTATGC GAAGATATCC 
ATTGGTGATG TAGGAAGCCG AGTTAAGCCT AAAAGCTATA GTCTCGTAGA CCCTGGAAAT 
GGCAGTGGCT TGAAGGTAGA CTATGCCTTT TTATCTGAAG TCTCTAATGT ATCTCCATTA 
CATGTTTGCG TAGAAGTTCT GTTTGAGAAC AGTTCTGCTG AACCTATCCT GGAGGTGAAT 
TTGGAAGATG AAGAATCAAT GAAGGTAGCA GACTCTTCTG AACAGACATT GGTTGGTAAA 
GCAAATGCAT CTTACAACAA TATACCAACC CTAATTCCAA TGGAGGAAAT TAGCTGCCTT 
GAACCACATC AGAGCACAAA GAGGCTTATC CAGGTTCGGT TTCATCACCA CCTGCTTCCC 
ATGAGGTTAA CCTTACATTA CAATGAAAAG AAAGTTCCTG TCAAGTTACG ACCTGATCTT 
GGATACCTTG TGAAACCATT TTCAATGAGC ATCGAGGAAT TCTTAGCTAC AGAATCTCGA 
TTACCTGGAA TGTTTGAGTA TAGTCGAAGG TGTACTTTTG ACGATCATGT TAAAGATTCA 
AGAACGGAGA ATGGGAAAGA CAAATTTCTT AGCATCTGCG AGAGTATAAC ACTAAAGGTG 
CTTAGCAACT CAAATCTACA CCTTGTATCT GTGGATTTAC CAGTTGCAAA CTCACTCGAA 
GATGCAACTG GTCTGCGATT AAGATTCAGC AGCAAAATCT TGAGCAGTGA GATCCCTCTT 
TTGATTACCA TCACAGTCGA AGGCAAATGC ACCGAAGTTC TGAATTTAAC AGTCAAGATC 
AATTGCGAAG AAACGGTTTT TGGTTTAAAC CTGTTGAACA GGATAGCTAA TTTCATGGTT 
GAGCCGTCTT CTTCAGCCAC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAT2 ( NM_202714.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_974443.4 Arabidopsis thaliana protein affected trafficking 2 (PAT2), mRNA.

Price & Availability↑

CloneID	OAb31661
Clone ID Related Accession (Same CDS sequence)	NM_202714.4
Accession Version	NM_202714.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	protein affected trafficking 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Jun 19, 2009 this sequence version replaced NM_202714.3.

ATGTTCAATA AGTTTGGTTC GACGTCGGAG ACTCTTAGCA AAGCTTCAGC TGGGTTGCTT 
CGGATCGGCA CTGACGCTCA TCTTTATGAT GATCCGGAAG ACGTCAACAT TGCTCCACTT 
CTCGATAGCA AGTTCGAATC TGAGAAATGT GAAGCTCTTA AGCGTCTCCT TGCTCTTATC 
GCTCAAGGCT TTGATGTCTC CAATTTCTTT CCTCAGGTGG TGAAGAATGT GGCGTCACAG 
TCATCGGAAG TGAAGAAGCT TGTTTACTTG TATTTGCTAC AATATGCAGA AAAGCGTCCA 
AATGAAGCAT TGCTATCGAT CAACTATTTT CAAAAGGACT TGGGGGATCC AAATCCATTG 
GTTAGGGCTT GGGCTCTTCG TACAATGGCT GGGATTCGCT TACATGTTAT TGCACCTCTT 
GCACTTGCAG CTGTGAGTAA ATGTGCTAGA GATCCAGCTG TTTATGTTCG GAGATGTGCT 
GCAAACGCTC TTCCGAAGTT GCATGATTTG CGGCTGGAAG AACATGCTTC TGCGATTGAA 
GAGCTAGTTG GGATACTGTT GAATGACCAT TCTCCAGGAG TAGTTGGAGC TGCGGCAGCG 
GCATTCACCT CTATCTGTCC AAATAACTTC AAACTGATTG GGAAGAACTA TAAGAAGTTG 
TGCCAGATTC TCCCTGACGT TGAAGAGTGG GGTCAGATAT TACTCATAGG AACTCTTTTG 
CGCTATGTGG TTGCTAGGCA TGGACTTGTA CGGGAATCTT TGATGTTGTC CATACATGGT 
ACGAATAGTA ATGGCTTCTG TGAGAAGGAT GGCCTAGGCA GAGATCTTAC ATTGGATAAA 
GAAGATGGTG GTAAAAGTGA TTCATTTGAT GTTAATCTGG TCAGTCTTGT ATCTAAATGT 
TACATTCAAG GTCCAGATGA GTATCTATCA AGATCCAGTT GCACCGATAC AGTGTCATCT 
GCTTTCGATA CCAAAGAGAC CACATCTATT GCACATAATG AAGATGTTAA GATCCTGCTT 
CAGTGTACAT CTCCATTGTT ATGGAGTAAT AACAGTGCGG TTGTACTAGC AGCAGCTGGC 
GTTCAATGGA TAATGGCGCC TCTGGAAGAT GTTAAGAAAA TTGTTAAACC GCTCTTGTTT 
TTGCTTAGGT CATCTAGCGC CTCCAAATAT GTGGTCCTCT GCAATATCCT AGTTTTTGCC 
AAAGCAGTCC CTTCTCTCTT TGCTCCACAC TTCGAAAATT TCTTTATTTG TTCCTCAGAT 
GCATATCAAG TGAAAGCATA CAAGCTGGAA ATGCTTTCTC TCATTGCTAC CACTTCATCT 
ATCGCTTCAA TTTTGAGAGA GTTTGAGGAT TATATTAAAG ATCCAGACAG GAGATTTGCG 
GCTGATACAG TTGCAGCAAT TGGTTTGTGC GCAAAAAGAC TGATGACAAT TCCAACTACA 
TGTCTTGATG GATTGTTAGC ACTAGTTCGA CAGGAATCTT TTGCTGGCGA TTTTGAGTCC 
GCGGATGGAG AAGCTGGAGT ATTGGTGCAA GCCGTGATGT CAATTCAGAC TATGATAGAG 
CGAGACCCCC TTCGTCATGA GAAAGTGTTA ATTCAGCTGT TTCGTAGTCT GGATTCAATC 
AAGGTAGCTG CTGCTCGTGC AACTATTATT TGGATGGTGG GGGTCTACTG CTCTTTGGGT 
CATATCATTC CTAGGATGCT GACCACAATA ACCAAGTACC TTGCATGGAG CTTTAAATCA 
GAGGCATCAG AGACAAAACT ACAGATTCTT AATACTATTG CTAAGGTTCT AATAAGTGCA 
GAGGCTGGTG ACTTTCATAT GTTGAAAAGG ATTGTGGTCT ATGTGTTTGA ATTGGGCGAA 
TATGACTTAA GCTATGATAT TCGTGATCGA ACTCGTTTCC TGAAGAAACT GCTGTCATGC 
AAATTAGCTA GTCATGAGCC GGCAGAAGAC AGTGTTGCAT CACAAGAAAA TATTGCTGCG 
CATGTTGTGG AACATGTCTT TGGAAGAAAA CTGAAATCTG TTTCACCTAT AACCCTGCAC 
AATCGTTTTT ATCTTCCTGG ATCTCTGTCA CAGATAGTTC TTCATGCAGC CCCTGGATAT 
GAACCTCTTC CAAAGCCCTG CAGTTTTGTG TATGAAGAAC AGGACCAGCT GTCAGATTTA 
GATAAACAAA GAGAGGCCGC AGCTGATTTA GATGGTTCTG AGGAATCCTC TGAAACAGGG 
GATGAGAATG GCTCCTCTGA TTATGACTCT GAATCTTCTA ATGGTTCGGA TTTTAGCAGT 
GAAGGTGATG AAAGAACAGT GTCTAATGAC GCCAATGACC CAGCTGCTCC TTTAATTCAA 
ATTTCAGAGA CTTCTGTTTC CGCTGACCAG GAAGAACTAA GGTCCAGAAG GGCCCTGGAT 
TTGTGGTTAG ATGATCAGCC TAGTACATCA AACCAAACTC CATCTGCATT AAATAGTAAT 
CAGAGCTCTT ATGCGAAGAT ATCCATTGGT GATGTAGGAA GCCGAGTTAA GCCTAAAAGC 
TATAGTCTCG TAGACCCTGG AAATGGCAGT GGCTTGAAGG TAGACTATGC CTTTTTATCT 
GAAGTCTCTA ATGTATCTCC ATTACATGTT TGCGTAGAAG TTCTGTTTGA GAACAGTTCT 
GCTGAACCTA TCCTGGAGGT GAATTTGGAA GATGAAGAAT CAATGAAGGT AGCAGACTCT 
TCTGAACAGA CATTGGTTGG TAAAGCAAAT GCATCTTACA ACAATATACC AACCCTAATT 
CCAATGGAGG AAATTAGCTG CCTTGAACCA CATCAGAGCA CAAAGAGGCT TATCCAGGTT 
CGGTTTCATC ACCACCTGCT TCCCATGAGG TTAACCTTAC ATTACAATGA AAAGAAAGTT 
CCTGTCAAGT TACGACCTGA TCTTGGATAC CTTGTGAAAC CATTTTCAAT GAGCATCGAG 
GAATTCTTAG CTACAGAATC TCGATTACCT GGAATGTTTG AGTATAGTCG AAGGTGTACT 
TTTGACGATC ATGTTAAAGA TTCAAGAACG GAGAATGGGA AAGACAAATT TCTTAGCATC 
TGCGAGAGTA TAACACTAAA GGTGCTTAGC AACTCAAATC TACACCTTGT ATCTGTGGAT 
TTACCAGTTG CAAACTCACT CGAAGATGCA ACTGGTCTGC GATTAAGATT CAGCAGCAAA 
ATCTTGAGCA GTGAGATCCC TCTTTTGATT ACCATCACAG TCGAAGGCAA ATGCACCGAA 
GTTCTGAATT TAACAGTCAA GATCAATTGC GAAGAAACGG TTTTTGGTTT AAACCTGTTG 
AACAGGATAG CTAATTTCAT GGTTGAGCCG TCTTCTTCAG CCACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_974443.4
CDS	78..3425
Translation	MFNKFGSTSETLSKASAGLLRIGTDAHLYDDPEDVNIAPLLDSKFESEKCEALKRLLALIAQGFDVSNFFPQVVKNVASQSSEVKKLVYLYLLQYAEKRPNEALLSINYFQKDLGDPNPLVRAWALRTMAGIRLHVIAPLALAAVSKCARDPAVYVRRCAANALPKLHDLRLEEHASAIEELVGILLNDHSPGVVGAAAAAFTSICPNNFKLIGKNYKKLCQILPDVEEWGQILLIGTLLRYVVARHGLVRESLMLSIHGTNSNGFCEKDGLGRDLTLDKEDGGKSDSFDVNLVSLVSKCYIQGPDEYLSRSSCTDTVSSAFDTKETTSIAHNEDVKILLQCTSPLLWSNNSAVVLAAAGVQWIMAPLEDVKKIVKPLLFLLRSSSASKYVVLCNILVFAKAVPSLFAPHFENFFICSSDAYQVKAYKLEMLSLIATTSSIASILREFEDYIKDPDRRFAADTVAAIGLCAKRLMTIPTTCLDGLLALVRQESFAGDFESADGEAGVLVQAVMSIQTMIERDPLRHEKVLIQLFRSLDSIKVAAARATIIWMVGVYCSLGHIIPRMLTTITKYLAWSFKSEASETKLQILNTIAKVLISAEAGDFHMLKRIVVYVFELGEYDLSYDIRDRTRFLKKLLSCKLASHEPAEDSVASQENIAAHVVEHVFGRKLKSVSPITLHNRFYLPGSLSQIVLHAAPGYEPLPKPCSFVYEEQDQLSDLDKQREAAADLDGSEESSETGDENGSSDYDSESSNGSDFSSEGDERTVSNDANDPAAPLIQISETSVSADQEELRSRRALDLWLDDQPSTSNQTPSALNSNQSSYAKISIGDVGSRVKPKSYSLVDPGNGSGLKVDYAFLSEVSNVSPLHVCVEVLFENSSAEPILEVNLEDEESMKVADSSEQTLVGKANASYNNIPTLIPMEEISCLEPHQSTKRLIQVRFHHHLLPMRLTLHYNEKKVPVKLRPDLGYLVKPFSMSIEEFLATESRLPGMFEYSRRCTFDDHVKDSRTENGKDKFLSICESITLKVLSNSNLHLVSVDLPVANSLEDATGLRLRFSSKILSSEIPLLITITVEGKCTEVLNLTVKINCEETVFGLNLLNRIANFMVEPSSSAT

Target ORF information:

RefSeq Version	NM_202714.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana protein affected trafficking 2 (PAT2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_202714.4

ORF Insert Sequence:

ATGTTCAATA AGTTTGGTTC GACGTCGGAG ACTCTTAGCA AAGCTTCAGC TGGGTTGCTT 
CGGATCGGCA CTGACGCTCA TCTTTATGAT GATCCGGAAG ACGTCAACAT TGCTCCACTT 
CTCGATAGCA AGTTCGAATC TGAGAAATGT GAAGCTCTTA AGCGTCTCCT TGCTCTTATC 
GCTCAAGGCT TTGATGTCTC CAATTTCTTT CCTCAGGTGG TGAAGAATGT GGCGTCACAG 
TCATCGGAAG TGAAGAAGCT TGTTTACTTG TATTTGCTAC AATATGCAGA AAAGCGTCCA 
AATGAAGCAT TGCTATCGAT CAACTATTTT CAAAAGGACT TGGGGGATCC AAATCCATTG 
GTTAGGGCTT GGGCTCTTCG TACAATGGCT GGGATTCGCT TACATGTTAT TGCACCTCTT 
GCACTTGCAG CTGTGAGTAA ATGTGCTAGA GATCCAGCTG TTTATGTTCG GAGATGTGCT 
GCAAACGCTC TTCCGAAGTT GCATGATTTG CGGCTGGAAG AACATGCTTC TGCGATTGAA 
GAGCTAGTTG GGATACTGTT GAATGACCAT TCTCCAGGAG TAGTTGGAGC TGCGGCAGCG 
GCATTCACCT CTATCTGTCC AAATAACTTC AAACTGATTG GGAAGAACTA TAAGAAGTTG 
TGCCAGATTC TCCCTGACGT TGAAGAGTGG GGTCAGATAT TACTCATAGG AACTCTTTTG 
CGCTATGTGG TTGCTAGGCA TGGACTTGTA CGGGAATCTT TGATGTTGTC CATACATGGT 
ACGAATAGTA ATGGCTTCTG TGAGAAGGAT GGCCTAGGCA GAGATCTTAC ATTGGATAAA 
GAAGATGGTG GTAAAAGTGA TTCATTTGAT GTTAATCTGG TCAGTCTTGT ATCTAAATGT 
TACATTCAAG GTCCAGATGA GTATCTATCA AGATCCAGTT GCACCGATAC AGTGTCATCT 
GCTTTCGATA CCAAAGAGAC CACATCTATT GCACATAATG AAGATGTTAA GATCCTGCTT 
CAGTGTACAT CTCCATTGTT ATGGAGTAAT AACAGTGCGG TTGTACTAGC AGCAGCTGGC 
GTTCAATGGA TAATGGCGCC TCTGGAAGAT GTTAAGAAAA TTGTTAAACC GCTCTTGTTT 
TTGCTTAGGT CATCTAGCGC CTCCAAATAT GTGGTCCTCT GCAATATCCT AGTTTTTGCC 
AAAGCAGTCC CTTCTCTCTT TGCTCCACAC TTCGAAAATT TCTTTATTTG TTCCTCAGAT 
GCATATCAAG TGAAAGCATA CAAGCTGGAA ATGCTTTCTC TCATTGCTAC CACTTCATCT 
ATCGCTTCAA TTTTGAGAGA GTTTGAGGAT TATATTAAAG ATCCAGACAG GAGATTTGCG 
GCTGATACAG TTGCAGCAAT TGGTTTGTGC GCAAAAAGAC TGATGACAAT TCCAACTACA 
TGTCTTGATG GATTGTTAGC ACTAGTTCGA CAGGAATCTT TTGCTGGCGA TTTTGAGTCC 
GCGGATGGAG AAGCTGGAGT ATTGGTGCAA GCCGTGATGT CAATTCAGAC TATGATAGAG 
CGAGACCCCC TTCGTCATGA GAAAGTGTTA ATTCAGCTGT TTCGTAGTCT GGATTCAATC 
AAGGTAGCTG CTGCTCGTGC AACTATTATT TGGATGGTGG GGGTCTACTG CTCTTTGGGT 
CATATCATTC CTAGGATGCT GACCACAATA ACCAAGTACC TTGCATGGAG CTTTAAATCA 
GAGGCATCAG AGACAAAACT ACAGATTCTT AATACTATTG CTAAGGTTCT AATAAGTGCA 
GAGGCTGGTG ACTTTCATAT GTTGAAAAGG ATTGTGGTCT ATGTGTTTGA ATTGGGCGAA 
TATGACTTAA GCTATGATAT TCGTGATCGA ACTCGTTTCC TGAAGAAACT GCTGTCATGC 
AAATTAGCTA GTCATGAGCC GGCAGAAGAC AGTGTTGCAT CACAAGAAAA TATTGCTGCG 
CATGTTGTGG AACATGTCTT TGGAAGAAAA CTGAAATCTG TTTCACCTAT AACCCTGCAC 
AATCGTTTTT ATCTTCCTGG ATCTCTGTCA CAGATAGTTC TTCATGCAGC CCCTGGATAT 
GAACCTCTTC CAAAGCCCTG CAGTTTTGTG TATGAAGAAC AGGACCAGCT GTCAGATTTA 
GATAAACAAA GAGAGGCCGC AGCTGATTTA GATGGTTCTG AGGAATCCTC TGAAACAGGG 
GATGAGAATG GCTCCTCTGA TTATGACTCT GAATCTTCTA ATGGTTCGGA TTTTAGCAGT 
GAAGGTGATG AAAGAACAGT GTCTAATGAC GCCAATGACC CAGCTGCTCC TTTAATTCAA 
ATTTCAGAGA CTTCTGTTTC CGCTGACCAG GAAGAACTAA GGTCCAGAAG GGCCCTGGAT 
TTGTGGTTAG ATGATCAGCC TAGTACATCA AACCAAACTC CATCTGCATT AAATAGTAAT 
CAGAGCTCTT ATGCGAAGAT ATCCATTGGT GATGTAGGAA GCCGAGTTAA GCCTAAAAGC 
TATAGTCTCG TAGACCCTGG AAATGGCAGT GGCTTGAAGG TAGACTATGC CTTTTTATCT 
GAAGTCTCTA ATGTATCTCC ATTACATGTT TGCGTAGAAG TTCTGTTTGA GAACAGTTCT 
GCTGAACCTA TCCTGGAGGT GAATTTGGAA GATGAAGAAT CAATGAAGGT AGCAGACTCT 
TCTGAACAGA CATTGGTTGG TAAAGCAAAT GCATCTTACA ACAATATACC AACCCTAATT 
CCAATGGAGG AAATTAGCTG CCTTGAACCA CATCAGAGCA CAAAGAGGCT TATCCAGGTT 
CGGTTTCATC ACCACCTGCT TCCCATGAGG TTAACCTTAC ATTACAATGA AAAGAAAGTT 
CCTGTCAAGT TACGACCTGA TCTTGGATAC CTTGTGAAAC CATTTTCAAT GAGCATCGAG 
GAATTCTTAG CTACAGAATC TCGATTACCT GGAATGTTTG AGTATAGTCG AAGGTGTACT 
TTTGACGATC ATGTTAAAGA TTCAAGAACG GAGAATGGGA AAGACAAATT TCTTAGCATC 
TGCGAGAGTA TAACACTAAA GGTGCTTAGC AACTCAAATC TACACCTTGT ATCTGTGGAT 
TTACCAGTTG CAAACTCACT CGAAGATGCA ACTGGTCTGC GATTAAGATT CAGCAGCAAA 
ATCTTGAGCA GTGAGATCCC TCTTTTGATT ACCATCACAG TCGAAGGCAA ATGCACCGAA 
GTTCTGAATT TAACAGTCAA GATCAATTGC GAAGAAACGG TTTTTGGTTT AAACCTGTTG 
AACAGGATAG CTAATTTCAT GGTTGAGCCG TCTTCTTCAG CCACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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PAT2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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