PGSIP1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	PGSIP1
Entrez Gene ID	821397
Full Name	plant glycogenin-like starch initiation protein 1
Synonyms	GUX1,glucuronic acid substitution of xylan 1,plant glycogenin-like starch initiation protein 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001202998.1	NP_001189927.1	plant glycogenin-like starch initiation protein 1
NM_112752.3	NP_566615.1	plant glycogenin-like starch initiation protein 1
NM_001035645.3	NP_001030722.1	plant glycogenin-like starch initiation protein 1

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	PGSIP1	NP_001030722.1
Oryza sativa (rice)	Os01g0880200	NP_001044991.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GUX1 and GUX2 are required for different patterns of 4-O-methylglucuronic acid substitution of xylan.
Title: GUX1 and GUX2 glucuronyltransferases decorate distinct domains of glucuronoxylan with different substitution patterns.

GUX2 and GUX4, have activity as xylan alpha-glucuronosyltransferases.
Title: Three members of the Arabidopsis glycosyltransferase family 8 are xylan glucuronosyltransferases.

GUX1/2/3 are glucuronyltransferases responsible for the glucuronic acid substitution of xylan, which is essential for normal secondary wall deposition and plant development.
Title: Arabidopsis GUX proteins are glucuronyltransferases responsible for the addition of glucuronic acid side chains onto xylan.

Data show that a mutant in the PGSIP1 gene encoding one of the Golgi localized candidate proteins has a highly decreased content of glucuronic acid in secondary cell walls and substantially reduced xylan glucuronosyltransferase activity.
Title: An integrative approach to the identification of Arabidopsis and rice genes involved in xylan and secondary wall development.

Data identify two Golgi-localized glycosyltransferases, GlucUronic acid substitution of Xylan (GUX)-1 and GUX2, that are required for addition of glucuronic acid and 4-O-methylglucuronic acid branches to xylan in Arabidopsis cell walls. [GUX1]
Title: Absence of branches from xylan in Arabidopsis gux mutants reveals potential for simplification of lignocellulosic biomass.

The following PGSIP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PGSIP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb11762	NM_001035645.3 Latest version!	Arabidopsis thaliana plant glycogenin-like starch initiation protein 1 (PGSIP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb11763	NM_112752.3 Latest version!	Arabidopsis thaliana plant glycogenin-like starch initiation protein 1 (PGSIP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb30123	NM_001202998.1 Latest version!	Arabidopsis thaliana plant glycogenin-like starch initiation protein 1 (PGSIP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PGSIP1 ( NM_001035645.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001030722.1 Arabidopsis thaliana plant glycogenin-like starch initiation protein 1 (PGSIP1), mRNA.

Price & Availability↑

CloneID	OAb11762
Clone ID Related Accession (Same CDS sequence)	NM_001035645.3
Accession Version	NM_001035645.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	plant glycogenin-like starch initiation protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_001035645.2.

ATGGCAAACT CTCCCGCTGC TCCTGCACCC ACCACCACAA CCGGTGGTGA CTCCCGGCGA 
CGCCTCTCCG CGTCCATAGA AGCAATATGC AAGAGGAGAT TCCGGAGAAA TAGCAAAGGA 
GGTGGCAGAT CGGATATGGT GAAACCGTTT AATATCATAA ATTTTTCGAC ACAAGACAAA 
AACAGTAGTT GTTGTTGTTT CACCAAGTTT CAGATCGTGA AGCTTCTCTT GTTTATCCTT 
CTCTCTGCCA CTCTCTTCAC CATTATCTAT TCTCCTGAAG CTTATCATCA TTCTCTTTCC 
CACTCATCTT CTCGATGGAT ATGGAGAAGA CAAGATCCAC GTTACTTCTC GGATCTGGAT 
ATAAACTGGG ACGATGTGAC TAAAACCCTT GAGAACATCG AAGAAGGCCG TACGATCGGT 
GTCTTGAATT TTGATTCGAA CGAGATCCAA CGATGGAGAG AAGTATCCAA GAGCAAGGAC 
AATGGGGATG AAGAAAAAGT TGTTGTATTG AATCTAGATT ACGCAGACAA GAATGTGACT 
TGGGACGCAC TATATCCAGA GTGGATCGAT GAGGAGCAAG AAACAGAGGT CCCTGTTTGT 
CCTAATATCC CGAACATTAA GGTACCTACA AGAAGACTCG ATCTGATCGT CGTGAAACTT 
CCTTGTCGGA AAGAAGGGAA TTGGTCGAGA GACGTCGGGA GATTGCATCT ACAGCTAGCG 
GCTGCAACTG TGGCGGCTTC GGCCAAAGGG TTTTTCAGGG GACATGTGTT TTTTGTATCT 
AGATGCTTTC CGATTCCGAA TCTTTTCCGG TGTAAAGATC TTGTGTCTCG GAGAGGCGAT 
GTTTGGTTGT ACAAACCTAA TCTTGATACC TTGAGAGACA AGCTTCAGCT GCCTGTAGGG 
TCTTGTGAGC TATCTCTTCC TCTTGGCATC CAAGATAGGC CAAGCTTAGG AAACCCTAAA 
AGAGAAGCTT ACGCAACAAT TCTTCACTCA GCTCACGTTT ACGTCTGCGG TGCAATCGCC 
GCGGCTCAGA GCATAAGACA GTCTGGTTCG ACGAGAGACC TTGTTATCCT TGTTGATGAC 
AACATCAGCG GTTACCACCG GAGTGGACTA GAAGCCGCGG GTTGGCAAAT CCGGACGATA 
CAGAGGATTC GAAACCCTAA GGCAGAGAAA GATGCTTACA ACGAATGGAA CTACAGCAAG 
TTCCGGCTAT GGCAGCTGAC TGATTACGAC AAAATCATTT TCATCGACGC GGATCTCTTA 
ATCTTGAGAA ACATCGATTT CTTGTTCTCG ATGCCTGAGA TCTCAGCTAC AGGAAACAAT 
GGAACTCTGT TTAATTCAGG AGTTATGGTG ATCGAGCCTT GCAACTGTAC GTTTCAGCTT 
CTGATGGAAC ATATAAACGA GATTGAGTCT TATAACGGTG GAGATCAAGG TTACTTAAAC 
GAGGTATTCA CATGGTGGCA CCGGATTCCA AAACATATGA ATTTCTTGAA GCATTTTTGG 
ATTGGCGATG AAGATGACGC GAAACGCAAG AAAACAGAGC TTTTTGGAGC AGAGCCTCCT 
GTTCTTTATG TTCTTCATTA CCTTGGGATG AAGCCGTGGT TATGTTACCG TGACTACGAC 
TGTAACTTCA ACTCCGACAT ATTCGTTGAG TTTGCTACCG ATATCGCTCA TCGAAAATGG 
TGGATGGTCC ACGACGCCAT GCCACAGGAA CTTCACCAAT TCTGTTACTT GCGATCCAAG 
CAAAAGGCAC AGCTGGAATA TGATCGCCGG CAAGCAGAGG CCGCAAATTA TGCCGACGGT 
CATTGGAAAA TAAGAGTAAA GGACCCGAGA TTCAAAATTT GCATCGACAA ATTATGTAAT 
TGGAAAAGTA TGCTGCGGCA TTGGGGCGAA TCAAATTGGA CTGACTACGA GTCTTTTGTT 
CCCACCCCAC CAGCCATTAC CGTAGACCGG AGATCATCAC TTCCCGGCCA TAACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001030722.1
CDS	183..2162
Translation	MANSPAAPAPTTTTGGDSRRRLSASIEAICKRRFRRNSKGGGRSDMVKPFNIINFSTQDKNSSCCCFTKFQIVKLLLFILLSATLFTIIYSPEAYHHSLSHSSSRWIWRRQDPRYFSDLDINWDDVTKTLENIEEGRTIGVLNFDSNEIQRWREVSKSKDNGDEEKVVVLNLDYADKNVTWDALYPEWIDEEQETEVPVCPNIPNIKVPTRRLDLIVVKLPCRKEGNWSRDVGRLHLQLAAATVAASAKGFFRGHVFFVSRCFPIPNLFRCKDLVSRRGDVWLYKPNLDTLRDKLQLPVGSCELSLPLGIQDRPSLGNPKREAYATILHSAHVYVCGAIAAAQSIRQSGSTRDLVILVDDNISGYHRSGLEAAGWQIRTIQRIRNPKAEKDAYNEWNYSKFRLWQLTDYDKIIFIDADLLILRNIDFLFSMPEISATGNNGTLFNSGVMVIEPCNCTFQLLMEHINEIESYNGGDQGYLNEVFTWWHRIPKHMNFLKHFWIGDEDDAKRKKTELFGAEPPVLYVLHYLGMKPWLCYRDYDCNFNSDIFVEFATDIAHRKWWMVHDAMPQELHQFCYLRSKQKAQLEYDRRQAEAANYADGHWKIRVKDPRFKICIDKLCNWKSMLRHWGESNWTDYESFVPTPPAITVDRRSSLPGHNL

Target ORF information:

RefSeq Version	NM_001035645.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana plant glycogenin-like starch initiation protein 1 (PGSIP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001035645.3

ORF Insert Sequence:

ATGGCAAACT CTCCCGCTGC TCCTGCACCC ACCACCACAA CCGGTGGTGA CTCCCGGCGA 
CGCCTCTCCG CGTCCATAGA AGCAATATGC AAGAGGAGAT TCCGGAGAAA TAGCAAAGGA 
GGTGGCAGAT CGGATATGGT GAAACCGTTT AATATCATAA ATTTTTCGAC ACAAGACAAA 
AACAGTAGTT GTTGTTGTTT CACCAAGTTT CAGATCGTGA AGCTTCTCTT GTTTATCCTT 
CTCTCTGCCA CTCTCTTCAC CATTATCTAT TCTCCTGAAG CTTATCATCA TTCTCTTTCC 
CACTCATCTT CTCGATGGAT ATGGAGAAGA CAAGATCCAC GTTACTTCTC GGATCTGGAT 
ATAAACTGGG ACGATGTGAC TAAAACCCTT GAGAACATCG AAGAAGGCCG TACGATCGGT 
GTCTTGAATT TTGATTCGAA CGAGATCCAA CGATGGAGAG AAGTATCCAA GAGCAAGGAC 
AATGGGGATG AAGAAAAAGT TGTTGTATTG AATCTAGATT ACGCAGACAA GAATGTGACT 
TGGGACGCAC TATATCCAGA GTGGATCGAT GAGGAGCAAG AAACAGAGGT CCCTGTTTGT 
CCTAATATCC CGAACATTAA GGTACCTACA AGAAGACTCG ATCTGATCGT CGTGAAACTT 
CCTTGTCGGA AAGAAGGGAA TTGGTCGAGA GACGTCGGGA GATTGCATCT ACAGCTAGCG 
GCTGCAACTG TGGCGGCTTC GGCCAAAGGG TTTTTCAGGG GACATGTGTT TTTTGTATCT 
AGATGCTTTC CGATTCCGAA TCTTTTCCGG TGTAAAGATC TTGTGTCTCG GAGAGGCGAT 
GTTTGGTTGT ACAAACCTAA TCTTGATACC TTGAGAGACA AGCTTCAGCT GCCTGTAGGG 
TCTTGTGAGC TATCTCTTCC TCTTGGCATC CAAGATAGGC CAAGCTTAGG AAACCCTAAA 
AGAGAAGCTT ACGCAACAAT TCTTCACTCA GCTCACGTTT ACGTCTGCGG TGCAATCGCC 
GCGGCTCAGA GCATAAGACA GTCTGGTTCG ACGAGAGACC TTGTTATCCT TGTTGATGAC 
AACATCAGCG GTTACCACCG GAGTGGACTA GAAGCCGCGG GTTGGCAAAT CCGGACGATA 
CAGAGGATTC GAAACCCTAA GGCAGAGAAA GATGCTTACA ACGAATGGAA CTACAGCAAG 
TTCCGGCTAT GGCAGCTGAC TGATTACGAC AAAATCATTT TCATCGACGC GGATCTCTTA 
ATCTTGAGAA ACATCGATTT CTTGTTCTCG ATGCCTGAGA TCTCAGCTAC AGGAAACAAT 
GGAACTCTGT TTAATTCAGG AGTTATGGTG ATCGAGCCTT GCAACTGTAC GTTTCAGCTT 
CTGATGGAAC ATATAAACGA GATTGAGTCT TATAACGGTG GAGATCAAGG TTACTTAAAC 
GAGGTATTCA CATGGTGGCA CCGGATTCCA AAACATATGA ATTTCTTGAA GCATTTTTGG 
ATTGGCGATG AAGATGACGC GAAACGCAAG AAAACAGAGC TTTTTGGAGC AGAGCCTCCT 
GTTCTTTATG TTCTTCATTA CCTTGGGATG AAGCCGTGGT TATGTTACCG TGACTACGAC 
TGTAACTTCA ACTCCGACAT ATTCGTTGAG TTTGCTACCG ATATCGCTCA TCGAAAATGG 
TGGATGGTCC ACGACGCCAT GCCACAGGAA CTTCACCAAT TCTGTTACTT GCGATCCAAG 
CAAAAGGCAC AGCTGGAATA TGATCGCCGG CAAGCAGAGG CCGCAAATTA TGCCGACGGT 
CATTGGAAAA TAAGAGTAAA GGACCCGAGA TTCAAAATTT GCATCGACAA ATTATGTAAT 
TGGAAAAGTA TGCTGCGGCA TTGGGGCGAA TCAAATTGGA CTGACTACGA GTCTTTTGTT 
CCCACCCCAC CAGCCATTAC CGTAGACCGG AGATCATCAC TTCCCGGCCA TAACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PGSIP1 ( NM_112752.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_566615.1 Arabidopsis thaliana plant glycogenin-like starch initiation protein 1 (PGSIP1), mRNA.

Price & Availability↑

CloneID	OAb11763
Clone ID Related Accession (Same CDS sequence)	NM_112752.3
Accession Version	NM_112752.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	plant glycogenin-like starch initiation protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Apr 18, 2007 this sequence version replaced NM_112752.2.

ATGGCAAACT CTCCCGCTGC TCCTGCACCC ACCACCACAA CCGGTGGTGA CTCCCGGCGA 
CGCCTCTCCG CGTCCATAGA AGCAATATGC AAGAGGAGAT TCCGGAGAAA TAGCAAAGGA 
GGTGGCAGAT CGGATATGGT GAAACCGTTT AATATCATAA ATTTTTCGAC ACAAGACAAA 
AACAGTAGTT GTTGTTGTTT CACCAAGTTT CAGATCGTGA AGCTTCTCTT GTTTATCCTT 
CTCTCTGCCA CTCTCTTCAC CATTATCTAT TCTCCTGAAG CTTATCATCA TTCTCTTTCC 
CACTCATCTT CTCGAAGACA AGATCCACGT TACTTCTCGG ATCTGGATAT AAACTGGGAC 
GATGTGACTA AAACCCTTGA GAACATCGAA GAAGGCCGTA CGATCGGTGT CTTGAATTTT 
GATTCGAACG AGATCCAACG ATGGAGAGAA GTATCCAAGA GCAAGGACAA TGGGGATGAA 
GAAAAAGTTG TTGTATTGAA TCTAGATTAC GCAGACAAGA ATGTGACTTG GGACGCACTA 
TATCCAGAGT GGATCGATGA GGAGCAAGAA ACAGAGGTCC CTGTTTGTCC TAATATCCCG 
AACATTAAGG TACCTACAAG AAGACTCGAT CTGATCGTCG TGAAACTTCC TTGTCGGAAA 
GAAGGGAATT GGTCGAGAGA CGTCGGGAGA TTGCATCTAC AGCTAGCGGC TGCAACTGTG 
GCGGCTTCGG CCAAAGGGTT TTTCAGGGGA CATGTGTTTT TTGTATCTAG ATGCTTTCCG 
ATTCCGAATC TTTTCCGGTG TAAAGATCTT GTGTCTCGGA GAGGCGATGT TTGGTTGTAC 
AAACCTAATC TTGATACCTT GAGAGACAAG CTTCAGCTGC CTGTAGGGTC TTGTGAGCTA 
TCTCTTCCTC TTGGCATCCA AGATAGGCCA AGCTTAGGAA ACCCTAAAAG AGAAGCTTAC 
GCAACAATTC TTCACTCAGC TCACGTTTAC GTCTGCGGTG CAATCGCCGC GGCTCAGAGC 
ATAAGACAGT CTGGTTCGAC GAGAGACCTT GTTATCCTTG TTGATGACAA CATCAGCGGT 
TACCACCGGA GTGGACTAGA AGCCGCGGGT TGGCAAATCC GGACGATACA GAGGATTCGA 
AACCCTAAGG CAGAGAAAGA TGCTTACAAC GAATGGAACT ACAGCAAGTT CCGGCTATGG 
CAGCTGACTG ATTACGACAA AATCATTTTC ATCGACGCGG ATCTCTTAAT CTTGAGAAAC 
ATCGATTTCT TGTTCTCGAT GCCTGAGATC TCAGCTACAG GAAACAATGG AACTCTGTTT 
AATTCAGGAG TTATGGTGAT CGAGCCTTGC AACTGTACGT TTCAGCTTCT GATGGAACAT 
ATAAACGAGA TTGAGTCTTA TAACGGTGGA GATCAAGGTT ACTTAAACGA GGTATTCACA 
TGGTGGCACC GGATTCCAAA ACATATGAAT TTCTTGAAGC ATTTTTGGAT TGGCGATGAA 
GATGACGCGA AACGCAAGAA AACAGAGCTT TTTGGAGCAG AGCCTCCTGT TCTTTATGTT 
CTTCATTACC TTGGGATGAA GCCGTGGTTA TGTTACCGTG ACTACGACTG TAACTTCAAC 
TCCGACATAT TCGTTGAGTT TGCTACCGAT ATCGCTCATC GAAAATGGTG GATGGTCCAC 
GACGCCATGC CACAGGAACT TCACCAATTC TGTTACTTGC GATCCAAGCA AAAGGCACAG 
CTGGAATATG ATCGCCGGCA AGCAGAGGCC GCAAATTATG CCGACGGTCA TTGGAAAATA 
AGAGTAAAGG ACCCGAGATT CAAAATTTGC ATCGACAAAT TATGTAATTG GAAAAGTATG 
CTGCGGCATT GGGGCGAATC AAATTGGACT GACTACGAGT CTTTTGTTCC CACCCCACCA 
GCCATTACCG TAGACCGGAG ATCATCACTT CCCGGCCATA ACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_566615.1
CDS	25..1992
Translation	MANSPAAPAPTTTTGGDSRRRLSASIEAICKRRFRRNSKGGGRSDMVKPFNIINFSTQDKNSSCCCFTKFQIVKLLLFILLSATLFTIIYSPEAYHHSLSHSSSRRQDPRYFSDLDINWDDVTKTLENIEEGRTIGVLNFDSNEIQRWREVSKSKDNGDEEKVVVLNLDYADKNVTWDALYPEWIDEEQETEVPVCPNIPNIKVPTRRLDLIVVKLPCRKEGNWSRDVGRLHLQLAAATVAASAKGFFRGHVFFVSRCFPIPNLFRCKDLVSRRGDVWLYKPNLDTLRDKLQLPVGSCELSLPLGIQDRPSLGNPKREAYATILHSAHVYVCGAIAAAQSIRQSGSTRDLVILVDDNISGYHRSGLEAAGWQIRTIQRIRNPKAEKDAYNEWNYSKFRLWQLTDYDKIIFIDADLLILRNIDFLFSMPEISATGNNGTLFNSGVMVIEPCNCTFQLLMEHINEIESYNGGDQGYLNEVFTWWHRIPKHMNFLKHFWIGDEDDAKRKKTELFGAEPPVLYVLHYLGMKPWLCYRDYDCNFNSDIFVEFATDIAHRKWWMVHDAMPQELHQFCYLRSKQKAQLEYDRRQAEAANYADGHWKIRVKDPRFKICIDKLCNWKSMLRHWGESNWTDYESFVPTPPAITVDRRSSLPGHNL

Target ORF information:

RefSeq Version	NM_112752.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana plant glycogenin-like starch initiation protein 1 (PGSIP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_112752.3

ORF Insert Sequence:

ATGGCAAACT CTCCCGCTGC TCCTGCACCC ACCACCACAA CCGGTGGTGA CTCCCGGCGA 
CGCCTCTCCG CGTCCATAGA AGCAATATGC AAGAGGAGAT TCCGGAGAAA TAGCAAAGGA 
GGTGGCAGAT CGGATATGGT GAAACCGTTT AATATCATAA ATTTTTCGAC ACAAGACAAA 
AACAGTAGTT GTTGTTGTTT CACCAAGTTT CAGATCGTGA AGCTTCTCTT GTTTATCCTT 
CTCTCTGCCA CTCTCTTCAC CATTATCTAT TCTCCTGAAG CTTATCATCA TTCTCTTTCC 
CACTCATCTT CTCGAAGACA AGATCCACGT TACTTCTCGG ATCTGGATAT AAACTGGGAC 
GATGTGACTA AAACCCTTGA GAACATCGAA GAAGGCCGTA CGATCGGTGT CTTGAATTTT 
GATTCGAACG AGATCCAACG ATGGAGAGAA GTATCCAAGA GCAAGGACAA TGGGGATGAA 
GAAAAAGTTG TTGTATTGAA TCTAGATTAC GCAGACAAGA ATGTGACTTG GGACGCACTA 
TATCCAGAGT GGATCGATGA GGAGCAAGAA ACAGAGGTCC CTGTTTGTCC TAATATCCCG 
AACATTAAGG TACCTACAAG AAGACTCGAT CTGATCGTCG TGAAACTTCC TTGTCGGAAA 
GAAGGGAATT GGTCGAGAGA CGTCGGGAGA TTGCATCTAC AGCTAGCGGC TGCAACTGTG 
GCGGCTTCGG CCAAAGGGTT TTTCAGGGGA CATGTGTTTT TTGTATCTAG ATGCTTTCCG 
ATTCCGAATC TTTTCCGGTG TAAAGATCTT GTGTCTCGGA GAGGCGATGT TTGGTTGTAC 
AAACCTAATC TTGATACCTT GAGAGACAAG CTTCAGCTGC CTGTAGGGTC TTGTGAGCTA 
TCTCTTCCTC TTGGCATCCA AGATAGGCCA AGCTTAGGAA ACCCTAAAAG AGAAGCTTAC 
GCAACAATTC TTCACTCAGC TCACGTTTAC GTCTGCGGTG CAATCGCCGC GGCTCAGAGC 
ATAAGACAGT CTGGTTCGAC GAGAGACCTT GTTATCCTTG TTGATGACAA CATCAGCGGT 
TACCACCGGA GTGGACTAGA AGCCGCGGGT TGGCAAATCC GGACGATACA GAGGATTCGA 
AACCCTAAGG CAGAGAAAGA TGCTTACAAC GAATGGAACT ACAGCAAGTT CCGGCTATGG 
CAGCTGACTG ATTACGACAA AATCATTTTC ATCGACGCGG ATCTCTTAAT CTTGAGAAAC 
ATCGATTTCT TGTTCTCGAT GCCTGAGATC TCAGCTACAG GAAACAATGG AACTCTGTTT 
AATTCAGGAG TTATGGTGAT CGAGCCTTGC AACTGTACGT TTCAGCTTCT GATGGAACAT 
ATAAACGAGA TTGAGTCTTA TAACGGTGGA GATCAAGGTT ACTTAAACGA GGTATTCACA 
TGGTGGCACC GGATTCCAAA ACATATGAAT TTCTTGAAGC ATTTTTGGAT TGGCGATGAA 
GATGACGCGA AACGCAAGAA AACAGAGCTT TTTGGAGCAG AGCCTCCTGT TCTTTATGTT 
CTTCATTACC TTGGGATGAA GCCGTGGTTA TGTTACCGTG ACTACGACTG TAACTTCAAC 
TCCGACATAT TCGTTGAGTT TGCTACCGAT ATCGCTCATC GAAAATGGTG GATGGTCCAC 
GACGCCATGC CACAGGAACT TCACCAATTC TGTTACTTGC GATCCAAGCA AAAGGCACAG 
CTGGAATATG ATCGCCGGCA AGCAGAGGCC GCAAATTATG CCGACGGTCA TTGGAAAATA 
AGAGTAAAGG ACCCGAGATT CAAAATTTGC ATCGACAAAT TATGTAATTG GAAAAGTATG 
CTGCGGCATT GGGGCGAATC AAATTGGACT GACTACGAGT CTTTTGTTCC CACCCCACCA 
GCCATTACCG TAGACCGGAG ATCATCACTT CCCGGCCATA ACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PGSIP1 ( NM_001202998.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001189927.1 Arabidopsis thaliana plant glycogenin-like starch initiation protein 1 (PGSIP1), mRNA.

Price & Availability↑

CloneID	OAb30123
Clone ID Related Accession (Same CDS sequence)	NM_001202998.1
Accession Version	NM_001202998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	plant glycogenin-like starch initiation protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074).

ATGGCAAACT CTCCCGCTGC TCCTGCACCC ACCACCACAA CCGGTGGTGA CTCCCGGCGA 
CGCCTCTCCG CGTCCATAGA AGCAATATGC AAGAGGAGAT TCCGGAGAAA TAGCAAAGGA 
GGTGGCAGAT CGGATATGGT GAAACCGTTT AATATCATAA ATTTTTCGAC ACAAGACAAA 
AACAGTAGTT GTTGTTGTTT CACCAAGTTT CAGATCGTGA AGCTTCTCTT GTTTATCCTT 
CTCTCTGCCA CTCTCTTCAC CATTATCTAT TCTCCTGAAG CTTATCATCA TTCTCTTTCC 
CACTCATCTT CTCGACAAGA TCCACGTTAC TTCTCGGATC TGGATATAAA CTGGGACGAT 
GTGACTAAAA CCCTTGAGAA CATCGAAGAA GGCCGTACGA TCGGTGTCTT GAATTTTGAT 
TCGAACGAGA TCCAACGATG GAGAGAAGTA TCCAAGAGCA AGGACAATGG GGATGAAGAA 
AAAGTTGTTG TATTGAATCT AGATTACGCA GACAAGAATG TGACTTGGGA CGCACTATAT 
CCAGAGTGGA TCGATGAGGA GCAAGAAACA GAGGTCCCTG TTTGTCCTAA TATCCCGAAC 
ATTAAGGTAC CTACAAGAAG ACTCGATCTG ATCGTCGTGA AACTTCCTTG TCGGAAAGAA 
GGGAATTGGT CGAGAGACGT CGGGAGATTG CATCTACAGC TAGCGGCTGC AACTGTGGCG 
GCTTCGGCCA AAGGGTTTTT CAGGGGACAT GTGTTTTTTG TATCTAGATG CTTTCCGATT 
CCGAATCTTT TCCGGTGTAA AGATCTTGTG TCTCGGAGAG GCGATGTTTG GTTGTACAAA 
CCTAATCTTG ATACCTTGAG AGACAAGCTT CAGCTGCCTG TAGGGTCTTG TGAGCTATCT 
CTTCCTCTTG GCATCCAAGA TAGGCCAAGC TTAGGAAACC CTAAAAGAGA AGCTTACGCA 
ACAATTCTTC ACTCAGCTCA CGTTTACGTC TGCGGTGCAA TCGCCGCGGC TCAGAGCATA 
AGACAGTCTG GTTCGACGAG AGACCTTGTT ATCCTTGTTG ATGACAACAT CAGCGGTTAC 
CACCGGAGTG GACTAGAAGC CGCGGGTTGG CAAATCCGGA CGATACAGAG GATTCGAAAC 
CCTAAGGCAG AGAAAGATGC TTACAACGAA TGGAACTACA GCAAGTTCCG GCTATGGCAG 
CTGACTGATT ACGACAAAAT CATTTTCATC GACGCGGATC TCTTAATCTT GAGAAACATC 
GATTTCTTGT TCTCGATGCC TGAGATCTCA GCTACAGGAA ACAATGGAAC TCTGTTTAAT 
TCAGGAGTTA TGGTGATCGA GCCTTGCAAC TGTACGTTTC AGCTTCTGAT GGAACATATA 
AACGAGATTG AGTCTTATAA CGGTGGAGAT CAAGGTTACT TAAACGAGGT ATTCACATGG 
TGGCACCGGA TTCCAAAACA TATGAATTTC TTGAAGCATT TTTGGATTGG CGATGAAGAT 
GACGCGAAAC GCAAGAAAAC AGAGCTTTTT GGAGCAGAGC CTCCTGTTCT TTATGTTCTT 
CATTACCTTG GGATGAAGCC GTGGTTATGT TACCGTGACT ACGACTGTAA CTTCAACTCC 
GACATATTCG TTGAGTTTGC TACCGATATC GCTCATCGAA AATGGTGGAT GGTCCACGAC 
GCCATGCCAC AGGAACTTCA CCAATTCTGT TACTTGCGAT CCAAGCAAAA GGCACAGCTG 
GAATATGATC GCCGGCAAGC AGAGGCCGCA AATTATGCCG ACGGTCATTG GAAAATAAGA 
GTAAAGGACC CGAGATTCAA AATTTGCATC GACAAATTAT GTAATTGGAA AAGTATGCTG 
CGGCATTGGG GCGAATCAAA TTGGACTGAC TACGAGTCTT TTGTTCCCAC CCCACCAGCC 
ATTACCGTAG ACCGGAGATC ATCACTTCCC GGCCATAACT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189927.1
CDS	44..2008
Translation	MANSPAAPAPTTTTGGDSRRRLSASIEAICKRRFRRNSKGGGRSDMVKPFNIINFSTQDKNSSCCCFTKFQIVKLLLFILLSATLFTIIYSPEAYHHSLSHSSSRQDPRYFSDLDINWDDVTKTLENIEEGRTIGVLNFDSNEIQRWREVSKSKDNGDEEKVVVLNLDYADKNVTWDALYPEWIDEEQETEVPVCPNIPNIKVPTRRLDLIVVKLPCRKEGNWSRDVGRLHLQLAAATVAASAKGFFRGHVFFVSRCFPIPNLFRCKDLVSRRGDVWLYKPNLDTLRDKLQLPVGSCELSLPLGIQDRPSLGNPKREAYATILHSAHVYVCGAIAAAQSIRQSGSTRDLVILVDDNISGYHRSGLEAAGWQIRTIQRIRNPKAEKDAYNEWNYSKFRLWQLTDYDKIIFIDADLLILRNIDFLFSMPEISATGNNGTLFNSGVMVIEPCNCTFQLLMEHINEIESYNGGDQGYLNEVFTWWHRIPKHMNFLKHFWIGDEDDAKRKKTELFGAEPPVLYVLHYLGMKPWLCYRDYDCNFNSDIFVEFATDIAHRKWWMVHDAMPQELHQFCYLRSKQKAQLEYDRRQAEAANYADGHWKIRVKDPRFKICIDKLCNWKSMLRHWGESNWTDYESFVPTPPAITVDRRSSLPGHNL

Target ORF information:

RefSeq Version	NM_001202998.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana plant glycogenin-like starch initiation protein 1 (PGSIP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202998.1

ORF Insert Sequence:

ATGGCAAACT CTCCCGCTGC TCCTGCACCC ACCACCACAA CCGGTGGTGA CTCCCGGCGA 
CGCCTCTCCG CGTCCATAGA AGCAATATGC AAGAGGAGAT TCCGGAGAAA TAGCAAAGGA 
GGTGGCAGAT CGGATATGGT GAAACCGTTT AATATCATAA ATTTTTCGAC ACAAGACAAA 
AACAGTAGTT GTTGTTGTTT CACCAAGTTT CAGATCGTGA AGCTTCTCTT GTTTATCCTT 
CTCTCTGCCA CTCTCTTCAC CATTATCTAT TCTCCTGAAG CTTATCATCA TTCTCTTTCC 
CACTCATCTT CTCGACAAGA TCCACGTTAC TTCTCGGATC TGGATATAAA CTGGGACGAT 
GTGACTAAAA CCCTTGAGAA CATCGAAGAA GGCCGTACGA TCGGTGTCTT GAATTTTGAT 
TCGAACGAGA TCCAACGATG GAGAGAAGTA TCCAAGAGCA AGGACAATGG GGATGAAGAA 
AAAGTTGTTG TATTGAATCT AGATTACGCA GACAAGAATG TGACTTGGGA CGCACTATAT 
CCAGAGTGGA TCGATGAGGA GCAAGAAACA GAGGTCCCTG TTTGTCCTAA TATCCCGAAC 
ATTAAGGTAC CTACAAGAAG ACTCGATCTG ATCGTCGTGA AACTTCCTTG TCGGAAAGAA 
GGGAATTGGT CGAGAGACGT CGGGAGATTG CATCTACAGC TAGCGGCTGC AACTGTGGCG 
GCTTCGGCCA AAGGGTTTTT CAGGGGACAT GTGTTTTTTG TATCTAGATG CTTTCCGATT 
CCGAATCTTT TCCGGTGTAA AGATCTTGTG TCTCGGAGAG GCGATGTTTG GTTGTACAAA 
CCTAATCTTG ATACCTTGAG AGACAAGCTT CAGCTGCCTG TAGGGTCTTG TGAGCTATCT 
CTTCCTCTTG GCATCCAAGA TAGGCCAAGC TTAGGAAACC CTAAAAGAGA AGCTTACGCA 
ACAATTCTTC ACTCAGCTCA CGTTTACGTC TGCGGTGCAA TCGCCGCGGC TCAGAGCATA 
AGACAGTCTG GTTCGACGAG AGACCTTGTT ATCCTTGTTG ATGACAACAT CAGCGGTTAC 
CACCGGAGTG GACTAGAAGC CGCGGGTTGG CAAATCCGGA CGATACAGAG GATTCGAAAC 
CCTAAGGCAG AGAAAGATGC TTACAACGAA TGGAACTACA GCAAGTTCCG GCTATGGCAG 
CTGACTGATT ACGACAAAAT CATTTTCATC GACGCGGATC TCTTAATCTT GAGAAACATC 
GATTTCTTGT TCTCGATGCC TGAGATCTCA GCTACAGGAA ACAATGGAAC TCTGTTTAAT 
TCAGGAGTTA TGGTGATCGA GCCTTGCAAC TGTACGTTTC AGCTTCTGAT GGAACATATA 
AACGAGATTG AGTCTTATAA CGGTGGAGAT CAAGGTTACT TAAACGAGGT ATTCACATGG 
TGGCACCGGA TTCCAAAACA TATGAATTTC TTGAAGCATT TTTGGATTGG CGATGAAGAT 
GACGCGAAAC GCAAGAAAAC AGAGCTTTTT GGAGCAGAGC CTCCTGTTCT TTATGTTCTT 
CATTACCTTG GGATGAAGCC GTGGTTATGT TACCGTGACT ACGACTGTAA CTTCAACTCC 
GACATATTCG TTGAGTTTGC TACCGATATC GCTCATCGAA AATGGTGGAT GGTCCACGAC 
GCCATGCCAC AGGAACTTCA CCAATTCTGT TACTTGCGAT CCAAGCAAAA GGCACAGCTG 
GAATATGATC GCCGGCAAGC AGAGGCCGCA AATTATGCCG ACGGTCATTG GAAAATAAGA 
GTAAAGGACC CGAGATTCAA AATTTGCATC GACAAATTAT GTAATTGGAA AAGTATGCTG 
CGGCATTGGG GCGAATCAAA TTGGACTGAC TACGAGTCTT TTGTTCCCAC CCCACCAGCC 
ATTACCGTAG ACCGGAGATC ATCACTTCCC GGCCATAACT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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PGSIP1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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