MBD9 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	MBD9
Entrez Gene ID	821132
Full Name	methyl-CPG-binding domain 9
Synonyms	ATMBD9,T13O15.10,T13O15_10,methyl-CPG-binding domain 9
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_111012.4	NP_186795.1	methyl-CPG-binding domain 9

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	MBD9	NP_186795.1
Oryza sativa (rice)	Os02g0192400	NP_001046163.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

AtMBD9 controls flowering time and axillary branching by modulating gene expression through DNA methylation and histone acetylation.
Title: AtMBD9 modulates Arabidopsis development through the dual epigenetic pathways of DNA methylation and histone acetylation.

The following MBD9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MBD9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb11572	NM_111012.4 Latest version!	Arabidopsis thaliana methyl-CPG-binding domain 9 (MBD9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MBD9 ( NM_111012.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_186795.1 Arabidopsis thaliana methyl-CPG-binding domain 9 (MBD9), mRNA.

Price & Availability↑

CloneID	OAb11572
Clone ID Related Accession (Same CDS sequence)	NM_111012.4
Accession Version	NM_111012.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	methyl-CPG-binding domain 9
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_111012.3.

ATGGAACCCA CTGATTCTAC TAACGAGCAA CTCGGAGACA CTAAGACCGC CGCTGTCAAG 
GAAGAGAGTC GCTCCTTTCT CGGCATCGAT CTCAACGAAA TCCCTACCGG CGCTACTCTC 
GGCGGTGGTT GCACCGCTGG TCAGGATGAC GACGGCGAAT ATGAACCTGT TGAAGTTGTT 
AGGTCAATTC ACGATAACCC GGACCCAGCC CCTGGAGCCC CTGCCGAGGT TCCTGAACCG 
GATCGGGATG CTTCGTGCGG CGCGTGTGGA AGACCTGAGT CTATAGAGCT CGTTGTAGTC 
TGCGATGCCT GTGAGCGAGG CTTTCATATG TCTTGTGTCA ACGATGGAGT TGAGGCGGCT 
CCTTCCGCCG ATTGGATGTG CAGCGACTGT CGTACTGGCG GCGAGAGGAG CAAACTGTGG 
CCGTTGGGGG TTAAGTCCAA GCTCATTCTG GACATGAACG CCTCGCCGCC CAGTGATGCT 
GAGGGATACG GAGCTGAGGA GACGTCTGAT TCGAGAAAGC ATATGCTCGC CAGCAGCTCT 
TGCATTGGAA ACTCTTTTGA TTATGCAATG ATGCATTCAA GCTTCTCAAG TCTCGGTAGA 
GGACATGCTA GTCTCGAAGC TTCAGGGTTA ATGTCTCGTA ATACTAAAAT GAGTATGGAT 
GCATTGGGTT CACATAATCT AGGTTTTGGA TTCCCATTAA ACTTGAACAA TAGTAGTTTG 
CCCATGAGAT TTCCATCCTT GGATCCAAGT GAGTTGTTTC TGCAAAATCT CAGGCATTTC 
ATATCTGAAA GGCATGGAGT ATTGGAAGAT GGCTGGCGTG TCGAATTCAG ACAACCTTTA 
AATGGTTATC AGTTATGTGC AGTGTATTGT GCTCCGAATG GAAAAACATT TAGTTCAATA 
CAAGAAGTTG CTTGTTATCT GGGCTTGGCA ATTAATGGTA ACTACAGCTG TATGGATGCT 
GAAATCAGGA ATGAGAATTC TCTTCTTCAA GAAAGATTGC ATACGCCCAA GAGAAGAAAG 
ACATCAAGAT GGCCAAACAA TGGTTTCCCT GAGCAAAAGG GTAGTTCAGT GAGTGCTCAA 
CTCAGGCGTT TTCCATTCAA TGGTCAGACC ATGTCTCCTT TCGCCGTTAA ATCTGGTACT 
CATTTTCAGG CTGGTGGTTC TCTTAGCTCT GGAAATAATG GATGCGGTTG TGAGGAAGCT 
AAGAATGGAT GTCCGATGCA GTTTGAAGAT TTCTTTGTTC TGTCGCTTGG ACGAATTGAC 
ATAAGACAGT CTTACCATAA TGTCAACGTG ATTTATCCAA TAGGATATAA GTCCTGCTGG 
CATGACAAGA TCACGGGGTC ACTATTTACA TGTGAAGTAT CTGATGGCAA TTCTGGTCCC 
ATTTTCAAGG TTACACGGTC ACCATGCTCG AAATCATTTA TTCCAGCTGG ATCAACTGTC 
TTCTCCTGCC CAAAGATTGA TGAAATGGTG GAACAGAACA GTGACAAACT TAGTAATCGT 
AGAGATAGTA CTCAAGAGCG TGATGATGAC GCTAGTGTTG AGATCCTTCT TTCGGAACAC 
TGCCCACCTC TTGGAGATGA TATATTGTCT TGTTTACGTG AGAAGAGTTT CTCCAAGACA 
GTCAATTCCC TGCGCTCAGA GGTTGATTCT TCTCGAGTAG ATTTTGATAA AAATTTATCC 
TATGATCAGG ACCATGGGGT TGAAATTGGT GACATTGTTG TGGAAGAAGA TTCATTGTCT 
GATGCATGGA AAAAAGTGTC TCAAAAACTT GTTGATGCAT GTTCAATTGT ACTGAAGCAG 
AAGGGTACCC TGAATTTCCT ATGTAAGCAT GTTGACAGAG AAACAAGTGA AATCAACTGG 
GATACCATGA ATGAGAAAGA CAATGTAATT TTATCGTTGT CAAAATTTTG CTGTTCGTTG 
GCTCCTTGCA GTGTCACGTG TGGTGAAAAG GATAAAAGCG AATTTGCAGC AGTAGTTGAT 
GCTTTGTCAA GGTGGCTCGA TCAAAACAGA TTTGGACTTG ATGCAGATTT TGTACAGGAA 
ATGATTGAAC ATATGCCTGG TGCCGAATCA TGTACGAATT ATAGGACTCT GAAGAGTAGA 
AGTTCTTCTT CTGTTCCTAT AACTGTAGCG GAAGGAGCGC TAGTGGTCAA ACCAAAAGGT 
GGGGAAAATG TCAAGGACGA AGTTTTCGGT GAGATTTCTC GGAAAGCCAA GAAGCCTAAA 
CTAAATGGTG GTCATGGTGT CAGAAATCTA CACCCTCCTC CTGGGAGGCC AATGTGTTTG 
AGGCTCCCTC CTGGGCTTGT TGGTGACTTC CTTCAGGTAT CTGAAGTGTT CTGGCGTTTC 
CATGAAATTT TGGGTTTTGA AGAGGCTTTC TCACCTGAAA ACCTTGAACA GGAGCTTATC 
AATCCAGTGT TTGATGGTTT GTTTCTTGAT AAACCTGGGA AAGATGATAA GAGAAGTGAG 
ATTAACTTTA CTGATAAGGA TTCTACAGCT ACTAAACTTT TTTCTTTGTT CGATGAATCT 
CGCCAACCTT TTCCTGCAAA AAATACCTCT GCTTCTGAAC TAAAGGAGAA AAAGGCAGGG 
GATTCTTCTG ATTTTAAGAT TTCAGATTCC TCTCGTGGGT CGTGTGTGGG TGCACTTCTA 
ACAAGGGCTC ACATTTCGCT TCTGCAAGTG CTAATATGTG AGCTGCAATC CAAGGTAGCT 
GCATTTGTTG ATCCAAACTT TGATTCTGGC GAATCGAGAT CCAGACGAGG ACGAAAAAAG 
GATGACAGTA CACTTTCTGC TAAAAGAAAT AAGCTGCATA TGCTTCCTGT TAATGAGTTC 
ACTTGGCCTG AATTGGCCCG TAGGTACATC TTGTCTCTTT TATCCATGGA TGGGAACCTC 
GAATCAGCAG AGATTGCTGC ACGTGAAAGT GGTAAGGTAT TCCGTTGCTT ACAAGGGGAT 
GGTGGTTTGC TTTGCGGCTC GCTTACAGGA GTGGCTGGGA TGGAAGCAGA TTCAATGTTA 
CTTGCAGAGG CTATTAAGAA AATATCTGGT TCGTTGACAA GCGAAAATGA TGTTCTTTCT 
GTGGAAGATG ATGATTCCGA TGGCCTTGAT GCTACTGAGA CAAACACTTG CAGTGGTGAT 
ATTCCAGAGT GGGCTCAGGT TTTGGAACCT GTGAAAAAGC TTCCAACAAA TGTTGGGACT 
AGAATCAGAA AGTGTGTCTA TGAAGCTTTA GAGAGAAATC CACCAGAGTG GGCAAAGAAG 
ATATTGGAGC ATTCTATCAG TAAAGAAATA TATAAAGGCA ATGCATCAGG ACCAACAAAG 
AAAGCTGTCC TCTCATTGCT AGCGGATATT CGAGGTGGAG ACTTGGTGCA GAGGTCTATT 
AAAGGAACCA AAAAGCGGAC ATATATAAGT GTATCTGATG TCATTATGAA GAAATGCCGT 
GCTGTATTGC GTGGTGTTGC AGCTGCAGAC GAGGATAAAG TCCTTTGCAC TTTACTGGGA 
AGAAAGTTAC TGAATTCCAG TGATAATGAT GATGACGGGC TCCTGGGATC ACCTGCAATG 
GTTTCGCGTC CCTTAGACTT CAGAACTATT GATTTGAGGT TGGCTGCTGG TGCGTATGAC 
GGATCAACTG AAGCTTTTCT TGAAGATGTT CTTGAGCTGT GGAGTAGTAT ACGTGTTATG 
TATGCAGATC AGCCCGATTG TGTGGACCTG GTTGCAACAT TGTCTGAAAA ATTCAAGTCG 
TTATACGAGG CTGAGGTTGT ACCACTTGTT CAGAAACTTA AGGACTACAG GAAATTGGAA 
TGCCTAAGTG CAGAGATGAA GAAGGAAATT AAGGACATAG TTGTTTCAGT AAATAAGCTT 
CCCAAGGCCC CGTGGGATGA GGGGGTATGT AAAGTATGTG GCGTTGACAA AGATGATGAC 
AGTGTTCTCT TGTGTGATAC ATGCGATGCT GAGTATCACA CATATTGTTT AAATCCACCT 
CTTATTAGAA TTCCTGATGG AAATTGGTAT TGTCCATCTT GTGTCATTGC CAAGCGCATG 
GCTCAAGAGG CTTTGGAATC TTACAAACTA GTTAGGCGGC GGAAAGGTAG AAAGTATCAG 
GGGGAACTCA CCCGAGCTTC TATGGAACTG ACTGCTCACC TGGCAGATGT GATGGAAGAA 
AAGGACTACT GGGAGTTTAG TGCTGAGGAG AGAATCTTGC TGCTTAAGCT TCTATGCGAT 
GAACTGCTTA GTTCATCTCT TGTCCATCAA CACCTTGAGC AGTGTGCCGA AGCAATAATT 
GAAATGCAGC AGAAGTTACG CTCTCTTTCC TCAGAATGGA AAAACGCAAA AATGCGGCAA 
GAATTCCTGA CGGCTAAACT GGCAAAGGTT GAACCGAGTA TTCTGAAGGA AGTGGGCGAA 
CCACATAATT CAAGCTACTT TGCAGACCAA ATGGGATGTG ATCCACAACC ACAGGAGGGC 
GTTGGAGACG GAGTTACTCG TGATGATGAG ACTTCCTCTA CTGCATATCT TAACAAGAAT 
CAAGGTAAAT CTCCACTTGA AACCGATACT CAACCCGGAG AGTCGCATGT TAATTTCGGT 
GAGAGCAAAA TTTCCTCCCC GGAAACAATA TCATCCCCTG GGAGGCATGA GCTACCTATA 
GCAGATACCT CTCCTCTTGT AACAGATAAT CTGCCTGAAA AAGATACCTC GGAGACCTTG 
CTTAAGTCAG TTGGAAGGAA TCATGAAACA CATTCACCAA ATTCCAATGC AGTAGAATTG 
CCGACAGCTC ATGATGCATC TTCTCAGGCT TCCCAAGAGT TGCAGGCTTG TCAGCAGGAT 
TTGAGTGCCA CTAGTAATGA AATTCAGAAT CTTCAGCAAT CAATTAGAAG CATAGAATCA 
CAGCTTCTAA AGCAATCTAT ACGGAGAGAT TTTCTGGGAA CCGATGCTAG TGGTCGGTTA 
TATTGGGGTT GCTGCTTCCC AGATGAAAAT CCTCGTATAT TGGTTGATGG AAGCATATCT 
TTGCAGAAAC CTGTTCAAGC CGATTTGATA GGTTCAAAAG TCCCCTCTCC GTTTCTCCAT 
ACCGTTGACC ATGGAAGACT AAGGCTTTCA CCCTGGACGT ATTATGAAAC TGAAACCGAG 
ATCAGTGAGC TTGTCCAATG GCTTCATGAT GATGATCTGA AAGAAAGAGA CCTGAGAGAG 
TCTATTTTGT GGTGGAAAAG GTTACGATAT GGAGACGTTC AAAAGGAAAA GAAACAAGCT 
CAGAATTTAT CTGCTCCGGT ATTTGCTACG GGTCTTGAGA CCAAGGCTGC CATGTCAATG 
GAGAAGAGAT ATGGTCCATG CATCAAACTG GAGATGGAAA CCTTAAAAAA ACGGGGGAAG 
AAGACAAAGG TTGCAGAGCG AGAGAAATTG TGTAGATGCG AATGCTTGGA ATCCATTTTG 
CCATCTATGA TTCACTGCCT CATATGCCAT AAAACATTCG CAAGTGATGA TGAGTTTGAG 
GATCACACTG AGAGTAAGTG TATTCCTTAT TCATTAGCAA CTGAAGAAGG CAAGGACATC 
TCTGATTCTT CAAAAGCCAA AGAAAGTCTG AAATCCGATT ATCTTAATGT AAAGTCTAGT 
GCCGGCAAAG ATGTAGCTGA AATATCCAAT GTTTCTGAAC TTGATTCTGG GTTGATAAGA 
TATCAAGAAG AAGAATCTAT TTCCCCATAC CATTTTGAGG AGATCTGTTC CAAGTTTGTG 
ACAAAGGATT GCAACAGAGA TTTGGTTAAA GAGATCGGTC TGATCAGTTC AAATGGCATT 
CCAACATTTC TTCCATCGTC ATCTACTCAT CTTAACGACT CCGTGCTCAT CTCTGCCAAA 
TCCAATAAGC CAGATGGTGG TGATTCAGGG GATCAGGTCA TTTTTGCTGG TCCTGAAACC 
AATGTTGAAG GCTTAAATTC TGAATCTAAC ATGTCATTCG ATAGATCTGT CACAGACAGT 
CACGGGGGTC CACTGGATAA ACCAAGTGGA CTGGGTTTTG GCTTCTCAGA GCAAAAGAAT 
AAGAAATCTT CAGGTAGTGG GTTGAAAAGC TGCTGTGTGG TTCCACAGGC TGCTTTGAAA 
CGAGTAACTG GCAAAGCTTT GCCGGGTTTC AGGTTCCTGA AAACCAACTT GCTTGATATG 
GATGTAGCAC TGCCTGAAGA AGCTTTAAGA CCATCGAAAT CACATCCAAA CCGTAGAAGA 
GCTTGGCGTG TATTTGTTAA ATCGTCGCAA AGTATATACG AGTTGGTTCA GGCAACAATT 
GTGGTAGAAG ATATGATTAA GACAGAGTAC TTGAAAAATG AATGGTGGTA CTGGTCTTCT 
CTTTCAGCGG CTGCTAAAAT CTCGACTCTC TCAGCGTTAT CCGTCCGTAT CTTCTCCCTC 
GACGCTGCTA TCATTTATGA TAAACCCATA ACTCCATCAA ATCCTATCGA TGAAACAAAG 
CCGATCATCA GCTTACCGGA CCAAAAGTCA CAGCCGGTTT CGGATTCTCA AGAAAGAAGC 
AGCAGAGTTA GAAGATCTGG CAAGAAAAGG AAAGAACCCG AGGGATCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_186795.1
CDS	127..6657
Translation	MEPTDSTNEQLGDTKTAAVKEESRSFLGIDLNEIPTGATLGGGCTAGQDDDGEYEPVEVVRSIHDNPDPAPGAPAEVPEPDRDASCGACGRPESIELVVVCDACERGFHMSCVNDGVEAAPSADWMCSDCRTGGERSKLWPLGVKSKLILDMNASPPSDAEGYGAEETSDSRKHMLASSSCIGNSFDYAMMHSSFSSLGRGHASLEASGLMSRNTKMSMDALGSHNLGFGFPLNLNNSSLPMRFPSLDPSELFLQNLRHFISERHGVLEDGWRVEFRQPLNGYQLCAVYCAPNGKTFSSIQEVACYLGLAINGNYSCMDAEIRNENSLLQERLHTPKRRKTSRWPNNGFPEQKGSSVSAQLRRFPFNGQTMSPFAVKSGTHFQAGGSLSSGNNGCGCEEAKNGCPMQFEDFFVLSLGRIDIRQSYHNVNVIYPIGYKSCWHDKITGSLFTCEVSDGNSGPIFKVTRSPCSKSFIPAGSTVFSCPKIDEMVEQNSDKLSNRRDSTQERDDDASVEILLSEHCPPLGDDILSCLREKSFSKTVNSLRSEVDSSRVDFDKNLSYDQDHGVEIGDIVVEEDSLSDAWKKVSQKLVDACSIVLKQKGTLNFLCKHVDRETSEINWDTMNEKDNVILSLSKFCCSLAPCSVTCGEKDKSEFAAVVDALSRWLDQNRFGLDADFVQEMIEHMPGAESCTNYRTLKSRSSSSVPITVAEGALVVKPKGGENVKDEVFGEISRKAKKPKLNGGHGVRNLHPPPGRPMCLRLPPGLVGDFLQVSEVFWRFHEILGFEEAFSPENLEQELINPVFDGLFLDKPGKDDKRSEINFTDKDSTATKLFSLFDESRQPFPAKNTSASELKEKKAGDSSDFKISDSSRGSCVGALLTRAHISLLQVLICELQSKVAAFVDPNFDSGESRSRRGRKKDDSTLSAKRNKLHMLPVNEFTWPELARRYILSLLSMDGNLESAEIAARESGKVFRCLQGDGGLLCGSLTGVAGMEADSMLLAEAIKKISGSLTSENDVLSVEDDDSDGLDATETNTCSGDIPEWAQVLEPVKKLPTNVGTRIRKCVYEALERNPPEWAKKILEHSISKEIYKGNASGPTKKAVLSLLADIRGGDLVQRSIKGTKKRTYISVSDVIMKKCRAVLRGVAAADEDKVLCTLLGRKLLNSSDNDDDGLLGSPAMVSRPLDFRTIDLRLAAGAYDGSTEAFLEDVLELWSSIRVMYADQPDCVDLVATLSEKFKSLYEAEVVPLVQKLKDYRKLECLSAEMKKEIKDIVVSVNKLPKAPWDEGVCKVCGVDKDDDSVLLCDTCDAEYHTYCLNPPLIRIPDGNWYCPSCVIAKRMAQEALESYKLVRRRKGRKYQGELTRASMELTAHLADVMEEKDYWEFSAEERILLLKLLCDELLSSSLVHQHLEQCAEAIIEMQQKLRSLSSEWKNAKMRQEFLTAKLAKVEPSILKEVGEPHNSSYFADQMGCDPQPQEGVGDGVTRDDETSSTAYLNKNQGKSPLETDTQPGESHVNFGESKISSPETISSPGRHELPIADTSPLVTDNLPEKDTSETLLKSVGRNHETHSPNSNAVELPTAHDASSQASQELQACQQDLSATSNEIQNLQQSIRSIESQLLKQSIRRDFLGTDASGRLYWGCCFPDENPRILVDGSISLQKPVQADLIGSKVPSPFLHTVDHGRLRLSPWTYYETETEISELVQWLHDDDLKERDLRESILWWKRLRYGDVQKEKKQAQNLSAPVFATGLETKAAMSMEKRYGPCIKLEMETLKKRGKKTKVAEREKLCRCECLESILPSMIHCLICHKTFASDDEFEDHTESKCIPYSLATEEGKDISDSSKAKESLKSDYLNVKSSAGKDVAEISNVSELDSGLIRYQEEESISPYHFEEICSKFVTKDCNRDLVKEIGLISSNGIPTFLPSSSTHLNDSVLISAKSNKPDGGDSGDQVIFAGPETNVEGLNSESNMSFDRSVTDSHGGPLDKPSGLGFGFSEQKNKKSSGSGLKSCCVVPQAALKRVTGKALPGFRFLKTNLLDMDVALPEEALRPSKSHPNRRRAWRVFVKSSQSIYELVQATIVVEDMIKTEYLKNEWWYWSSLSAAAKISTLSALSVRIFSLDAAIIYDKPITPSNPIDETKPIISLPDQKSQPVSDSQERSSRVRRSGKKRKEPEGS

Target ORF information:

RefSeq Version	NM_111012.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana methyl-CPG-binding domain 9 (MBD9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_111012.4

ORF Insert Sequence:

ATGGAACCCA CTGATTCTAC TAACGAGCAA CTCGGAGACA CTAAGACCGC CGCTGTCAAG 
GAAGAGAGTC GCTCCTTTCT CGGCATCGAT CTCAACGAAA TCCCTACCGG CGCTACTCTC 
GGCGGTGGTT GCACCGCTGG TCAGGATGAC GACGGCGAAT ATGAACCTGT TGAAGTTGTT 
AGGTCAATTC ACGATAACCC GGACCCAGCC CCTGGAGCCC CTGCCGAGGT TCCTGAACCG 
GATCGGGATG CTTCGTGCGG CGCGTGTGGA AGACCTGAGT CTATAGAGCT CGTTGTAGTC 
TGCGATGCCT GTGAGCGAGG CTTTCATATG TCTTGTGTCA ACGATGGAGT TGAGGCGGCT 
CCTTCCGCCG ATTGGATGTG CAGCGACTGT CGTACTGGCG GCGAGAGGAG CAAACTGTGG 
CCGTTGGGGG TTAAGTCCAA GCTCATTCTG GACATGAACG CCTCGCCGCC CAGTGATGCT 
GAGGGATACG GAGCTGAGGA GACGTCTGAT TCGAGAAAGC ATATGCTCGC CAGCAGCTCT 
TGCATTGGAA ACTCTTTTGA TTATGCAATG ATGCATTCAA GCTTCTCAAG TCTCGGTAGA 
GGACATGCTA GTCTCGAAGC TTCAGGGTTA ATGTCTCGTA ATACTAAAAT GAGTATGGAT 
GCATTGGGTT CACATAATCT AGGTTTTGGA TTCCCATTAA ACTTGAACAA TAGTAGTTTG 
CCCATGAGAT TTCCATCCTT GGATCCAAGT GAGTTGTTTC TGCAAAATCT CAGGCATTTC 
ATATCTGAAA GGCATGGAGT ATTGGAAGAT GGCTGGCGTG TCGAATTCAG ACAACCTTTA 
AATGGTTATC AGTTATGTGC AGTGTATTGT GCTCCGAATG GAAAAACATT TAGTTCAATA 
CAAGAAGTTG CTTGTTATCT GGGCTTGGCA ATTAATGGTA ACTACAGCTG TATGGATGCT 
GAAATCAGGA ATGAGAATTC TCTTCTTCAA GAAAGATTGC ATACGCCCAA GAGAAGAAAG 
ACATCAAGAT GGCCAAACAA TGGTTTCCCT GAGCAAAAGG GTAGTTCAGT GAGTGCTCAA 
CTCAGGCGTT TTCCATTCAA TGGTCAGACC ATGTCTCCTT TCGCCGTTAA ATCTGGTACT 
CATTTTCAGG CTGGTGGTTC TCTTAGCTCT GGAAATAATG GATGCGGTTG TGAGGAAGCT 
AAGAATGGAT GTCCGATGCA GTTTGAAGAT TTCTTTGTTC TGTCGCTTGG ACGAATTGAC 
ATAAGACAGT CTTACCATAA TGTCAACGTG ATTTATCCAA TAGGATATAA GTCCTGCTGG 
CATGACAAGA TCACGGGGTC ACTATTTACA TGTGAAGTAT CTGATGGCAA TTCTGGTCCC 
ATTTTCAAGG TTACACGGTC ACCATGCTCG AAATCATTTA TTCCAGCTGG ATCAACTGTC 
TTCTCCTGCC CAAAGATTGA TGAAATGGTG GAACAGAACA GTGACAAACT TAGTAATCGT 
AGAGATAGTA CTCAAGAGCG TGATGATGAC GCTAGTGTTG AGATCCTTCT TTCGGAACAC 
TGCCCACCTC TTGGAGATGA TATATTGTCT TGTTTACGTG AGAAGAGTTT CTCCAAGACA 
GTCAATTCCC TGCGCTCAGA GGTTGATTCT TCTCGAGTAG ATTTTGATAA AAATTTATCC 
TATGATCAGG ACCATGGGGT TGAAATTGGT GACATTGTTG TGGAAGAAGA TTCATTGTCT 
GATGCATGGA AAAAAGTGTC TCAAAAACTT GTTGATGCAT GTTCAATTGT ACTGAAGCAG 
AAGGGTACCC TGAATTTCCT ATGTAAGCAT GTTGACAGAG AAACAAGTGA AATCAACTGG 
GATACCATGA ATGAGAAAGA CAATGTAATT TTATCGTTGT CAAAATTTTG CTGTTCGTTG 
GCTCCTTGCA GTGTCACGTG TGGTGAAAAG GATAAAAGCG AATTTGCAGC AGTAGTTGAT 
GCTTTGTCAA GGTGGCTCGA TCAAAACAGA TTTGGACTTG ATGCAGATTT TGTACAGGAA 
ATGATTGAAC ATATGCCTGG TGCCGAATCA TGTACGAATT ATAGGACTCT GAAGAGTAGA 
AGTTCTTCTT CTGTTCCTAT AACTGTAGCG GAAGGAGCGC TAGTGGTCAA ACCAAAAGGT 
GGGGAAAATG TCAAGGACGA AGTTTTCGGT GAGATTTCTC GGAAAGCCAA GAAGCCTAAA 
CTAAATGGTG GTCATGGTGT CAGAAATCTA CACCCTCCTC CTGGGAGGCC AATGTGTTTG 
AGGCTCCCTC CTGGGCTTGT TGGTGACTTC CTTCAGGTAT CTGAAGTGTT CTGGCGTTTC 
CATGAAATTT TGGGTTTTGA AGAGGCTTTC TCACCTGAAA ACCTTGAACA GGAGCTTATC 
AATCCAGTGT TTGATGGTTT GTTTCTTGAT AAACCTGGGA AAGATGATAA GAGAAGTGAG 
ATTAACTTTA CTGATAAGGA TTCTACAGCT ACTAAACTTT TTTCTTTGTT CGATGAATCT 
CGCCAACCTT TTCCTGCAAA AAATACCTCT GCTTCTGAAC TAAAGGAGAA AAAGGCAGGG 
GATTCTTCTG ATTTTAAGAT TTCAGATTCC TCTCGTGGGT CGTGTGTGGG TGCACTTCTA 
ACAAGGGCTC ACATTTCGCT TCTGCAAGTG CTAATATGTG AGCTGCAATC CAAGGTAGCT 
GCATTTGTTG ATCCAAACTT TGATTCTGGC GAATCGAGAT CCAGACGAGG ACGAAAAAAG 
GATGACAGTA CACTTTCTGC TAAAAGAAAT AAGCTGCATA TGCTTCCTGT TAATGAGTTC 
ACTTGGCCTG AATTGGCCCG TAGGTACATC TTGTCTCTTT TATCCATGGA TGGGAACCTC 
GAATCAGCAG AGATTGCTGC ACGTGAAAGT GGTAAGGTAT TCCGTTGCTT ACAAGGGGAT 
GGTGGTTTGC TTTGCGGCTC GCTTACAGGA GTGGCTGGGA TGGAAGCAGA TTCAATGTTA 
CTTGCAGAGG CTATTAAGAA AATATCTGGT TCGTTGACAA GCGAAAATGA TGTTCTTTCT 
GTGGAAGATG ATGATTCCGA TGGCCTTGAT GCTACTGAGA CAAACACTTG CAGTGGTGAT 
ATTCCAGAGT GGGCTCAGGT TTTGGAACCT GTGAAAAAGC TTCCAACAAA TGTTGGGACT 
AGAATCAGAA AGTGTGTCTA TGAAGCTTTA GAGAGAAATC CACCAGAGTG GGCAAAGAAG 
ATATTGGAGC ATTCTATCAG TAAAGAAATA TATAAAGGCA ATGCATCAGG ACCAACAAAG 
AAAGCTGTCC TCTCATTGCT AGCGGATATT CGAGGTGGAG ACTTGGTGCA GAGGTCTATT 
AAAGGAACCA AAAAGCGGAC ATATATAAGT GTATCTGATG TCATTATGAA GAAATGCCGT 
GCTGTATTGC GTGGTGTTGC AGCTGCAGAC GAGGATAAAG TCCTTTGCAC TTTACTGGGA 
AGAAAGTTAC TGAATTCCAG TGATAATGAT GATGACGGGC TCCTGGGATC ACCTGCAATG 
GTTTCGCGTC CCTTAGACTT CAGAACTATT GATTTGAGGT TGGCTGCTGG TGCGTATGAC 
GGATCAACTG AAGCTTTTCT TGAAGATGTT CTTGAGCTGT GGAGTAGTAT ACGTGTTATG 
TATGCAGATC AGCCCGATTG TGTGGACCTG GTTGCAACAT TGTCTGAAAA ATTCAAGTCG 
TTATACGAGG CTGAGGTTGT ACCACTTGTT CAGAAACTTA AGGACTACAG GAAATTGGAA 
TGCCTAAGTG CAGAGATGAA GAAGGAAATT AAGGACATAG TTGTTTCAGT AAATAAGCTT 
CCCAAGGCCC CGTGGGATGA GGGGGTATGT AAAGTATGTG GCGTTGACAA AGATGATGAC 
AGTGTTCTCT TGTGTGATAC ATGCGATGCT GAGTATCACA CATATTGTTT AAATCCACCT 
CTTATTAGAA TTCCTGATGG AAATTGGTAT TGTCCATCTT GTGTCATTGC CAAGCGCATG 
GCTCAAGAGG CTTTGGAATC TTACAAACTA GTTAGGCGGC GGAAAGGTAG AAAGTATCAG 
GGGGAACTCA CCCGAGCTTC TATGGAACTG ACTGCTCACC TGGCAGATGT GATGGAAGAA 
AAGGACTACT GGGAGTTTAG TGCTGAGGAG AGAATCTTGC TGCTTAAGCT TCTATGCGAT 
GAACTGCTTA GTTCATCTCT TGTCCATCAA CACCTTGAGC AGTGTGCCGA AGCAATAATT 
GAAATGCAGC AGAAGTTACG CTCTCTTTCC TCAGAATGGA AAAACGCAAA AATGCGGCAA 
GAATTCCTGA CGGCTAAACT GGCAAAGGTT GAACCGAGTA TTCTGAAGGA AGTGGGCGAA 
CCACATAATT CAAGCTACTT TGCAGACCAA ATGGGATGTG ATCCACAACC ACAGGAGGGC 
GTTGGAGACG GAGTTACTCG TGATGATGAG ACTTCCTCTA CTGCATATCT TAACAAGAAT 
CAAGGTAAAT CTCCACTTGA AACCGATACT CAACCCGGAG AGTCGCATGT TAATTTCGGT 
GAGAGCAAAA TTTCCTCCCC GGAAACAATA TCATCCCCTG GGAGGCATGA GCTACCTATA 
GCAGATACCT CTCCTCTTGT AACAGATAAT CTGCCTGAAA AAGATACCTC GGAGACCTTG 
CTTAAGTCAG TTGGAAGGAA TCATGAAACA CATTCACCAA ATTCCAATGC AGTAGAATTG 
CCGACAGCTC ATGATGCATC TTCTCAGGCT TCCCAAGAGT TGCAGGCTTG TCAGCAGGAT 
TTGAGTGCCA CTAGTAATGA AATTCAGAAT CTTCAGCAAT CAATTAGAAG CATAGAATCA 
CAGCTTCTAA AGCAATCTAT ACGGAGAGAT TTTCTGGGAA CCGATGCTAG TGGTCGGTTA 
TATTGGGGTT GCTGCTTCCC AGATGAAAAT CCTCGTATAT TGGTTGATGG AAGCATATCT 
TTGCAGAAAC CTGTTCAAGC CGATTTGATA GGTTCAAAAG TCCCCTCTCC GTTTCTCCAT 
ACCGTTGACC ATGGAAGACT AAGGCTTTCA CCCTGGACGT ATTATGAAAC TGAAACCGAG 
ATCAGTGAGC TTGTCCAATG GCTTCATGAT GATGATCTGA AAGAAAGAGA CCTGAGAGAG 
TCTATTTTGT GGTGGAAAAG GTTACGATAT GGAGACGTTC AAAAGGAAAA GAAACAAGCT 
CAGAATTTAT CTGCTCCGGT ATTTGCTACG GGTCTTGAGA CCAAGGCTGC CATGTCAATG 
GAGAAGAGAT ATGGTCCATG CATCAAACTG GAGATGGAAA CCTTAAAAAA ACGGGGGAAG 
AAGACAAAGG TTGCAGAGCG AGAGAAATTG TGTAGATGCG AATGCTTGGA ATCCATTTTG 
CCATCTATGA TTCACTGCCT CATATGCCAT AAAACATTCG CAAGTGATGA TGAGTTTGAG 
GATCACACTG AGAGTAAGTG TATTCCTTAT TCATTAGCAA CTGAAGAAGG CAAGGACATC 
TCTGATTCTT CAAAAGCCAA AGAAAGTCTG AAATCCGATT ATCTTAATGT AAAGTCTAGT 
GCCGGCAAAG ATGTAGCTGA AATATCCAAT GTTTCTGAAC TTGATTCTGG GTTGATAAGA 
TATCAAGAAG AAGAATCTAT TTCCCCATAC CATTTTGAGG AGATCTGTTC CAAGTTTGTG 
ACAAAGGATT GCAACAGAGA TTTGGTTAAA GAGATCGGTC TGATCAGTTC AAATGGCATT 
CCAACATTTC TTCCATCGTC ATCTACTCAT CTTAACGACT CCGTGCTCAT CTCTGCCAAA 
TCCAATAAGC CAGATGGTGG TGATTCAGGG GATCAGGTCA TTTTTGCTGG TCCTGAAACC 
AATGTTGAAG GCTTAAATTC TGAATCTAAC ATGTCATTCG ATAGATCTGT CACAGACAGT 
CACGGGGGTC CACTGGATAA ACCAAGTGGA CTGGGTTTTG GCTTCTCAGA GCAAAAGAAT 
AAGAAATCTT CAGGTAGTGG GTTGAAAAGC TGCTGTGTGG TTCCACAGGC TGCTTTGAAA 
CGAGTAACTG GCAAAGCTTT GCCGGGTTTC AGGTTCCTGA AAACCAACTT GCTTGATATG 
GATGTAGCAC TGCCTGAAGA AGCTTTAAGA CCATCGAAAT CACATCCAAA CCGTAGAAGA 
GCTTGGCGTG TATTTGTTAA ATCGTCGCAA AGTATATACG AGTTGGTTCA GGCAACAATT 
GTGGTAGAAG ATATGATTAA GACAGAGTAC TTGAAAAATG AATGGTGGTA CTGGTCTTCT 
CTTTCAGCGG CTGCTAAAAT CTCGACTCTC TCAGCGTTAT CCGTCCGTAT CTTCTCCCTC 
GACGCTGCTA TCATTTATGA TAAACCCATA ACTCCATCAA ATCCTATCGA TGAAACAAAG 
CCGATCATCA GCTTACCGGA CCAAAAGTCA CAGCCGGTTT CGGATTCTCA AGAAAGAAGC 
AGCAGAGTTA GAAGATCTGG CAAGAAAAGG AAAGAACCCG AGGGATCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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MBD9 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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