NRPD1B cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	NRPD1B
Entrez Gene ID	818591
Full Name	nuclear RNA polymerase D1B
Synonyms	ATNRPD1B,DEFECTIVE IN MERISTEM SILENCING 5,DMS5,DRD3,NRPE1,T28M21.19,T28M21_19,nuclear RNA polymerase D1B
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001336818.1	NP_001323985.1	nuclear RNA polymerase D1B
NM_129561.4	NP_181532.2	nuclear RNA polymerase D1B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Lin X, Kaul S, Rounsley S, Shea TP, Benito MI, Town CD, Fujii CY, Mason T, Bowman CL, Barnstead M, Feldblyum TV, Buell CR, Ketchum KA, Lee J, Ronning CM, Koo HL, Moffat KS, Cronin LA, Shen M, Pai G, Van Aken S, Umayam L, Tallon LJ, Gill JE, Adams MD, Carrera AJ, Creasy TH, Goodman HM, Somerville CR, Copenhaver GP, Preuss D, Nierman WC, White O, Eisen JA, Salzberg SL, Fraser CM, Venter JC
Nature402(6763)761-8(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Genome-wide chromatin association of NRPE1 is dependent on all members of a putative chromatin-remodeling complex termed DDR, which includes the proteins DRD1, DMS3 and RDM1.
Title: DDR complex facilitates global association of RNA polymerase V to promoters and evolutionarily young transposons.

DRD1-Pol V-dependent de novo methylation may play a role in restraining the inappropriate silencing of active protein-coding genes in plants. [NRPE1]
Title: DRD1-Pol V-dependent self-silencing of an exogenous silencer restricts the non-cell autonomous silencing of an endogenous target gene.

Mutants defective for the NRPD1a and NRPD1b alternative largest subunits of polymerase IV were tested for their ability to undergo RNA silencing and mantenance of transcription and post-transcriptional silencing.
Title: NRPD1a and NRPD1b are required to maintain post-transcriptional RNA silencing and RNA-directed DNA methylation in Arabidopsis.

The following NRPD1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NRPD1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb09291	NM_129561.4 Latest version!	Arabidopsis thaliana nuclear RNA polymerase D1B (NRPD1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OAb36467	NM_001336818.1 Latest version!	Arabidopsis thaliana nuclear RNA polymerase D1B (NRPD1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NRPD1B ( NM_129561.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_181532.2 Arabidopsis thaliana nuclear RNA polymerase D1B (NRPD1B), mRNA.

Price & Availability↑

CloneID	OAb09291
Clone ID Related Accession (Same CDS sequence)	NM_129561.4
Accession Version	NM_129561.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	nuclear RNA polymerase D1B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). On Sep 12, 2016 this sequence version replaced NM_129561.3.

ATGGAGGAAG AATCTACATC AGAGATTCTT GACGGAGAGA TTGTTGGAAT CACATTTGCT 
CTTGCTAGTC ACCATGAAAT ATGTATTCAA TCGATTAGTG AATCCGCAAT TAATCATCCC 
AGTCAACTCA CTAACGCATT CTTGGGGTTA CCTCTTGAAT TTGGTAAATG TGAATCTTGC 
GGTGCGACAG AACCAGATAA ATGTGAAGGT CATTTTGGTT ATATTCAGCT TCCAGTGCCA 
ATATACCATC CTGCTCATGT TAATGAGTTG AAACAAATGC TGAGCCTTCT GTGCTTGAAA 
TGTCTGAAGA TAAAGAAGGC AAAGGGCACA AGTGGTGGTC TGGCGGATCG CTTGTTGGGT 
GTCTGTTGTG AGGAAGCTTC GCAAATCTCA ATCAAAGATA GAGCATCAGA TGGTGCCTCA 
TATCTAGAAC TGAAGCTCCC ATCTAGGTCA AGACTGCAGC CTGGTTGCTG GAATTTCCTG 
GAAAGATATG GTTATCGCTA TGGAAGCGAT TACACGCGTC CCCTGCTGGC AAGAGAGGTT 
AAGGAAATTC TAAGACGGAT CCCCGAAGAA TCAAGAAAGA AACTCACGGC AAAGGGACAT 
ATTCCTCAAG AAGGATACAT ACTAGAGTAT CTTCCTGTCC CGCCTAACTG TTTGTCAGTG 
CCAGAGGCTT CTGATGGTTT CAGCACCATG TCAGTGGATC CTTCCAGAAT TGAGCTAAAG 
GACGTTCTGA AGAAAGTCAT AGCCATAAAA AGCTCTAGGT CTGGCGAGAC AAACTTTGAG 
TCGCATAAGG CTGAAGCCAG TGAGATGTTT AGAGTGGTGG ATACCTATCT CCAGGTGAGG 
GGTACTGCAA AAGCGGCAAG AAACATTGAT ATGAGATACG GGGTGTCTAA GATCTCGGAT 
AGTTCATCTT CAAAAGCTTG GACAGAAAAA ATGAGAACAC TTTTCATCCG TAAGGGTTCA 
GGTTTCTCTT CCCGTAGCGT CATCACTGGA GATGCTTACC GACATGTGAA TGAGGTAGGG 
ATCCCAATAG AAATTGCGCA GAGAATCACA TTTGAGGAAA GGGTTAGTGT CCACAATAGA 
GGTTATCTAC AAAAGTTGGT AGACGACAAG TTGTGTTTGA GTTATACTCA AGGTTCTACG 
ACCTATTCTT TAAGGGATGG ATCCAAGGGG CACACTGAGC TTAAACCCGG GCAGGTTGTT 
CATCGCAGGG TCATGGATGG GGATGTTGTG TTTATCAACA GACCCCCGAC AACACATAAA 
CATTCTCTTC AAGCGCTTCG GGTGTATGTT CATGAAGACA ACACCGTGAA GATTAACCCT 
CTGATGTGTA GCCCCCTCAG TGCTGATTTT GATGGTGATT GTGTCCATTT GTTCTACCCT 
CAGTCTCTTA GTGCTAAGGC AGAGGTTATG GAGCTCTTCT CTGTGGAGAA GCAACTGCTT 
AGCTCACATA CTGGACAGCT GATTTTGCAG ATGGGTAGTG ATTCTTTACT GTCTTTGAGG 
GTAATGCTGG AGCGGGTATT TCTGGACAAG GCAACTGCTC AACAGTTAGC TATGTATGGC 
TCTCTGTCCC TGCCACCACC AGCACTAAGG AAGTCCAGTA AATCTGGCCC AGCTTGGACA 
GTTTTCCAGA TTCTACAGCT TGCCTTTCCA GAACGTCTGA GTTGCAAAGG AGACAGATTC 
CTGGTTGATG GAAGTGATTT GCTGAAGTTT GATTTCGGGG TTGACGCGAT GGGGTCCATC 
ATCAATGAAA TTGTAACTTC AATCTTCCTG GAGAAGGGTC CAAAGGAGAC ACTTGGATTC 
TTTGATTCGC TGCAGCCTCT ATTGATGGAA AGTCTGTTTG CTGAGGGATT TAGTCTAAGT 
TTGGAAGACT TATCCATGTC TAGGGCAGAC ATGGATGTAA TTCACAACCT AATAATCCGA 
GAGATTTCAC CTATGGTGTC TCGCTTGAGA TTATCATATA GAGATGAATT GCAGCTAGAG 
AACAGCATCC ACAAAGTGAA GGAAGTGGCT GCTAACTTTA TGCTGAAGTC ATATTCCATT 
CGGAACTTGA TTGATATCAA GAGTAACTCA GCAATCACCA AGCTGGTGCA GCAGACAGGC 
TTCTTGGGTC TTCAGCTTTC TGACAAGAAG AAGTTTTACA CGAAGACTCT GGTAGAGGAC 
ATGGCTATCT TTTGTAAGAG AAAGTATGGC AGGATTTCTT CATCTGGTGA TTTTGGAATA 
GTGAAGGGCT GCTTCTTTCA TGGGCTGGAT CCGTATGAAG AGATGGCTCA TTCAATTGCA 
GCTAGGGAGG TAATTGTTCG CTCTTCAAGG GGATTAGCTG AGCCTGGAAC CCTCTTTAAG 
AACCTGATGG CTGTTCTTCG GGATATCGTG ATTACAAACG ATGGAACTGT AAGAAACACC 
TGTAGTAACT CAGTCATCCA GTTCAAATAT GGAGTTGATT CTGAACGTGG ACATCAAGGT 
CTGTTTGAAG CTGGAGAACC TGTTGGAGTA TTGGCAGCTA CTGCCATGTC AAATCCTGCA 
TATAAGGCAG TGCTTGATTC CTCTCCAAAT AGTAATTCAT CATGGGAGCT AATGAAGGAA 
GTTCTGCTCT GCAAAGTCAA TTTCCAAAAC ACTACTAATG ATCGGCGCGT AATCCTTTAT 
TTAAACGAAT GCCATTGTGG AAAAAGGTTT TGCCAGGAAA ATGCTGCTTG CACAGTCAGG 
AACAAGCTGA ATAAAGTTAG TCTTAAAGAT ACTGCAGTGG AATTCCTAGT CGAATATAGA 
AAGCAACCAA CTATATCAGA AATTTTTGGA ATTGATTCAT GCCTACATGG CCATATTCAT 
CTTAATAAGA CATTGCTGCA AGATTGGAAC ATCAGTATGC AGGATATACA TCAAAAGTGT 
GAGGATGTAA TTAACTCACT AGGACAAAAG AAAAAGAAGA AAGCTACTGA TGATTTCAAG 
AGAACAAGTT TGTCTGTCAG TGAGTGCTGC TCTTTCCGAG ATCCTTGTGG GAGCAAAGGT 
TCGGATATGC CTTGTTTGAC GTTCTCCTAC AATGCCACCG ATCCCGATCT AGAACGAACT 
CTTGATGTTC TTTGCAATAC AGTTTACCCA GTTCTGCTGG AAATAGTGAT CAAAGGTGAT 
TCACGGATTT GCTCAGCAAA TATAATCTGG AATAGTTCAG ACATGACAAC GTGGATCCGG 
AATCGTCATG CATCCCGCCG AGGGGAATGG GTTTTGGATG TCACTGTTGA AAAATCTGCT 
GTCAAGCAAA GTGGTGATGC ATGGAGGGTT GTGATAGACT CATGTCTTTC AGTGCTTCAT 
CTAATAGACA CAAAACGGTC AATCCCTTAT TCTGTAAAAC AAGTGCAAGA ATTGCTTGGG 
TTATCCTGTG CTTTTGAGCA AGCAGTACAG CGTCTATCAG CTTCAGTGAG AATGGTCTCC 
AAGGGAGTTT TGAAAGAACA CATCATTCTC CTAGCAAACA ACATGACTTG TTCAGGGACT 
ATGTTGGGTT TCAATTCTGG AGGTTACAAA GCTTTGACCC GATCCTTAAA CATCAAGGCT 
CCATTCACAG AAGCAACTCT GATTGCACCA AGAAAATGTT TTGAGAAAGC GGCTGAGAAA 
TGTCATACAG ATTCACTATC AACAGTCGTT GGGTCTTGTT CTTGGGGAAA ACGCGTGGAT 
GTTGGTACAG GCTCACAGTT TGAGCTCCTA TGGAACCAAA AAGAGACTGG TCTAGATGAT 
AAAGAAGAAA CAGATGTGTA CAGCTTCCTT CAAATGGTCA TATCCACGAC AAACGCAGAT 
GCATTTGTTT CTTCCCCTGG TTTTGATGTT ACAGAGGAAG AAATGGCTGA ATGGGCTGAA 
TCGCCCGAGC GAGACTCTGC TCTTGGAGAG CCCAAGTTTG AGGACAGTGC CGACTTCCAA 
AATTTACATG ACGAAGGCAA GCCATCAGGA GCCAACTGGG AGAAAAGCTC TTCGTGGGAC 
AATGGTTGCA GTGGTGGTTC AGAGTGGGGA GTCTCAAAAA GTACAGGTGG TGAGGCAAAC 
CCGGAGTCTA ATTGGGAAAA GACTACAAAT GTTGAGAAGG AAGATGCTTG GTCTAGTTGG 
AACACTAGAA AAGATGCTCA AGAATCTTCC AAGTCAGATT CAGGGGGTGC TTGGGGTATA 
AAGACAAAGG ATGCTGATGC AGATACAACA CCAAACTGGG AAACAAGTCC AGCTCCGAAG 
GATTCCATTG TCCCAGAAAA TAATGAGCCG ACTTCTGACG TTTGGGGACA CAAATCTGTT 
TCAGACAAAT CGTGGGACAA AAAGAACTGG GGAACTGAAT CAGCTCCTGC TGCATGGGGT 
TCTACAGATG CTGCTGTTTG GGGTTCTAGT GATAAAAAGA ACTCGGAAAC AGAATCAGAT 
GCTGCTGCTT GGGGTTCCAG GGACAAAAAT AATTCTGACG TTGGGTCAGG TGCTGGTGTT 
TTAGGTCCCT GGAACAAGAA GAGCTCGGAA ACTGAGTCAA ATGGTGCTAC TTGGGGTTCT 
AGTGACAAAA CCAAATCAGG TGCTGCTGCA TGGAATTCTT GGGACAAAAA GAACATAGAA 
ACTGACTCAG AACCTGCTGC TTGGGGTTCT CAGGGAAAAA AGAATTCTGA AACTGAGTCA 
GGTCCTGCTG CTTGGGGTGC TTGGGACAAA AAGAAATCGG AAACTGAACC AGGTCCTGCT 
GGTTGGGGTA TGGGGGACAA AAAGAATTCT GAAACTGAAT TAGGCCCTGC TGCTATGGGT 
AATTGGGACA AAAAGAAATC GGATACGAAA TCAGGTCCAG CTGCTTGGGG ATCTACAGAT 
GCTGCTGCTT GGGGTTCTAG TGACAAAAAC AACTCGGAAA CTGAATCAGA TGCTGCTGCT 
TGGGGTTCTA GGAACAAAAA GACTTCTGAA ATTGAATCAG GTGCTGGCGC TTGGGGTTCG 
TGGGGACAGC CAAGTCCCAC TGCAGAAGAC AAGGATACCA ATGAGGATGA TAGAAATCCG 
TGGGTTTCCT TGAAGGAGAC CAAAAGTAGA GAGAAGGATG ATAAAGAGAG AAGCCAGTGG 
GGAAATCCAG CCAAGAAATT TCCATCTAGT GGTGGCTGGT CCAATGGTGG TGGTGCAGAC 
TGGAAAGGAA ATAGGAACCA TACTCCTAGG CCACCTAGAT CTGAGGATAA TTTGGCTCCC 
ATGTTTACAG CAACGAGACA GCGCTTGGAC TCATTCACCT CTGAGGAGCA AGAGCTGCTC 
TCAGATGTTG AGCCAGTCAT GAGGACTCTC AGGAAAATTA TGCATCCGTC TGCGTACCCT 
GATGGAGACC CAATTTCTGA TGATGACAAG ACGTTTGTCC TGGAGAAGAT TTTGAACTTC 
CATCCCCAAA AAGAGACAAA GCTTGGTTCT GGCGTTGACT TCATCACGGT TGATAAGCAC 
ACCATCTTCT CAGACAGCAG GTGCTTCTTT GTTGTCTCAA CTGATGGGGC CAAACAGGAC 
TTTTCATACC GAAAATCCCT CAACAACTAT TTGATGAAGA AATACCCTGA CCGTGCTGAA 
GAATTCATTG ACAAGTACTT TACGAAACCT CGGCCTAGCG GAAACAGAGA CAGGAACAAC 
CAAGATGCCA CACCACCTGG GGAAGAACAG TCTCAGCCTC CGAATCAGTC TATAGGTAAT 
GGAGGTGACG ACTTTCAGAC ACAGACTCAG TCTCAGTCTC CATCTCAGAC TCGGGCTCAG 
TCTCCATCTC AGGCTCAGGC TCAGTCTCCA TCTCAGACGC AGTCTCAGTC TCAGTCTCAG 
TCTCAGTCTC AGTCTCAGTC TCAGTCTCAG TCTCAGTCTC AGTCTCAGTC TCAGTCTCAG 
TCTCAGTCTC AGTCTCAGTC TCCATCCCAG ACTCAGACTC AGTCTCCGTC TCAGACTCAG 
GCTCAGGCTC AGTCTCCATC ATCTCAGTCT CCGTCCCAGA CGCAGACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_181532.2
CDS	275..6205
Translation	MEEESTSEILDGEIVGITFALASHHEICIQSISESAINHPSQLTNAFLGLPLEFGKCESCGATEPDKCEGHFGYIQLPVPIYHPAHVNELKQMLSLLCLKCLKIKKAKGTSGGLADRLLGVCCEEASQISIKDRASDGASYLELKLPSRSRLQPGCWNFLERYGYRYGSDYTRPLLAREVKEILRRIPEESRKKLTAKGHIPQEGYILEYLPVPPNCLSVPEASDGFSTMSVDPSRIELKDVLKKVIAIKSSRSGETNFESHKAEASEMFRVVDTYLQVRGTAKAARNIDMRYGVSKISDSSSSKAWTEKMRTLFIRKGSGFSSRSVITGDAYRHVNEVGIPIEIAQRITFEERVSVHNRGYLQKLVDDKLCLSYTQGSTTYSLRDGSKGHTELKPGQVVHRRVMDGDVVFINRPPTTHKHSLQALRVYVHEDNTVKINPLMCSPLSADFDGDCVHLFYPQSLSAKAEVMELFSVEKQLLSSHTGQLILQMGSDSLLSLRVMLERVFLDKATAQQLAMYGSLSLPPPALRKSSKSGPAWTVFQILQLAFPERLSCKGDRFLVDGSDLLKFDFGVDAMGSIINEIVTSIFLEKGPKETLGFFDSLQPLLMESLFAEGFSLSLEDLSMSRADMDVIHNLIIREISPMVSRLRLSYRDELQLENSIHKVKEVAANFMLKSYSIRNLIDIKSNSAITKLVQQTGFLGLQLSDKKKFYTKTLVEDMAIFCKRKYGRISSSGDFGIVKGCFFHGLDPYEEMAHSIAAREVIVRSSRGLAEPGTLFKNLMAVLRDIVITNDGTVRNTCSNSVIQFKYGVDSERGHQGLFEAGEPVGVLAATAMSNPAYKAVLDSSPNSNSSWELMKEVLLCKVNFQNTTNDRRVILYLNECHCGKRFCQENAACTVRNKLNKVSLKDTAVEFLVEYRKQPTISEIFGIDSCLHGHIHLNKTLLQDWNISMQDIHQKCEDVINSLGQKKKKKATDDFKRTSLSVSECCSFRDPCGSKGSDMPCLTFSYNATDPDLERTLDVLCNTVYPVLLEIVIKGDSRICSANIIWNSSDMTTWIRNRHASRRGEWVLDVTVEKSAVKQSGDAWRVVIDSCLSVLHLIDTKRSIPYSVKQVQELLGLSCAFEQAVQRLSASVRMVSKGVLKEHIILLANNMTCSGTMLGFNSGGYKALTRSLNIKAPFTEATLIAPRKCFEKAAEKCHTDSLSTVVGSCSWGKRVDVGTGSQFELLWNQKETGLDDKEETDVYSFLQMVISTTNADAFVSSPGFDVTEEEMAEWAESPERDSALGEPKFEDSADFQNLHDEGKPSGANWEKSSSWDNGCSGGSEWGVSKSTGGEANPESNWEKTTNVEKEDAWSSWNTRKDAQESSKSDSGGAWGIKTKDADADTTPNWETSPAPKDSIVPENNEPTSDVWGHKSVSDKSWDKKNWGTESAPAAWGSTDAAVWGSSDKKNSETESDAAAWGSRDKNNSDVGSGAGVLGPWNKKSSETESNGATWGSSDKTKSGAAAWNSWDKKNIETDSEPAAWGSQGKKNSETESGPAAWGAWDKKKSETEPGPAGWGMGDKKNSETELGPAAMGNWDKKKSDTKSGPAAWGSTDAAAWGSSDKNNSETESDAAAWGSRNKKTSEIESGAGAWGSWGQPSPTAEDKDTNEDDRNPWVSLKETKSREKDDKERSQWGNPAKKFPSSGGWSNGGGADWKGNRNHTPRPPRSEDNLAPMFTATRQRLDSFTSEEQELLSDVEPVMRTLRKIMHPSAYPDGDPISDDDKTFVLEKILNFHPQKETKLGSGVDFITVDKHTIFSDSRCFFVVSTDGAKQDFSYRKSLNNYLMKKYPDRAEEFIDKYFTKPRPSGNRDRNNQDATPPGEEQSQPPNQSIGNGGDDFQTQTQSQSPSQTRAQSPSQAQAQSPSQTQSQSQSQSQSQSQSQSQSQSQSQSQSQSQSQSQSPSQTQTQSPSQTQAQAQSPSSQSPSQTQT

Target ORF information:

RefSeq Version	NM_129561.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana nuclear RNA polymerase D1B (NRPD1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_129561.4

ORF Insert Sequence:

ATGGAGGAAG AATCTACATC AGAGATTCTT GACGGAGAGA TTGTTGGAAT CACATTTGCT 
CTTGCTAGTC ACCATGAAAT ATGTATTCAA TCGATTAGTG AATCCGCAAT TAATCATCCC 
AGTCAACTCA CTAACGCATT CTTGGGGTTA CCTCTTGAAT TTGGTAAATG TGAATCTTGC 
GGTGCGACAG AACCAGATAA ATGTGAAGGT CATTTTGGTT ATATTCAGCT TCCAGTGCCA 
ATATACCATC CTGCTCATGT TAATGAGTTG AAACAAATGC TGAGCCTTCT GTGCTTGAAA 
TGTCTGAAGA TAAAGAAGGC AAAGGGCACA AGTGGTGGTC TGGCGGATCG CTTGTTGGGT 
GTCTGTTGTG AGGAAGCTTC GCAAATCTCA ATCAAAGATA GAGCATCAGA TGGTGCCTCA 
TATCTAGAAC TGAAGCTCCC ATCTAGGTCA AGACTGCAGC CTGGTTGCTG GAATTTCCTG 
GAAAGATATG GTTATCGCTA TGGAAGCGAT TACACGCGTC CCCTGCTGGC AAGAGAGGTT 
AAGGAAATTC TAAGACGGAT CCCCGAAGAA TCAAGAAAGA AACTCACGGC AAAGGGACAT 
ATTCCTCAAG AAGGATACAT ACTAGAGTAT CTTCCTGTCC CGCCTAACTG TTTGTCAGTG 
CCAGAGGCTT CTGATGGTTT CAGCACCATG TCAGTGGATC CTTCCAGAAT TGAGCTAAAG 
GACGTTCTGA AGAAAGTCAT AGCCATAAAA AGCTCTAGGT CTGGCGAGAC AAACTTTGAG 
TCGCATAAGG CTGAAGCCAG TGAGATGTTT AGAGTGGTGG ATACCTATCT CCAGGTGAGG 
GGTACTGCAA AAGCGGCAAG AAACATTGAT ATGAGATACG GGGTGTCTAA GATCTCGGAT 
AGTTCATCTT CAAAAGCTTG GACAGAAAAA ATGAGAACAC TTTTCATCCG TAAGGGTTCA 
GGTTTCTCTT CCCGTAGCGT CATCACTGGA GATGCTTACC GACATGTGAA TGAGGTAGGG 
ATCCCAATAG AAATTGCGCA GAGAATCACA TTTGAGGAAA GGGTTAGTGT CCACAATAGA 
GGTTATCTAC AAAAGTTGGT AGACGACAAG TTGTGTTTGA GTTATACTCA AGGTTCTACG 
ACCTATTCTT TAAGGGATGG ATCCAAGGGG CACACTGAGC TTAAACCCGG GCAGGTTGTT 
CATCGCAGGG TCATGGATGG GGATGTTGTG TTTATCAACA GACCCCCGAC AACACATAAA 
CATTCTCTTC AAGCGCTTCG GGTGTATGTT CATGAAGACA ACACCGTGAA GATTAACCCT 
CTGATGTGTA GCCCCCTCAG TGCTGATTTT GATGGTGATT GTGTCCATTT GTTCTACCCT 
CAGTCTCTTA GTGCTAAGGC AGAGGTTATG GAGCTCTTCT CTGTGGAGAA GCAACTGCTT 
AGCTCACATA CTGGACAGCT GATTTTGCAG ATGGGTAGTG ATTCTTTACT GTCTTTGAGG 
GTAATGCTGG AGCGGGTATT TCTGGACAAG GCAACTGCTC AACAGTTAGC TATGTATGGC 
TCTCTGTCCC TGCCACCACC AGCACTAAGG AAGTCCAGTA AATCTGGCCC AGCTTGGACA 
GTTTTCCAGA TTCTACAGCT TGCCTTTCCA GAACGTCTGA GTTGCAAAGG AGACAGATTC 
CTGGTTGATG GAAGTGATTT GCTGAAGTTT GATTTCGGGG TTGACGCGAT GGGGTCCATC 
ATCAATGAAA TTGTAACTTC AATCTTCCTG GAGAAGGGTC CAAAGGAGAC ACTTGGATTC 
TTTGATTCGC TGCAGCCTCT ATTGATGGAA AGTCTGTTTG CTGAGGGATT TAGTCTAAGT 
TTGGAAGACT TATCCATGTC TAGGGCAGAC ATGGATGTAA TTCACAACCT AATAATCCGA 
GAGATTTCAC CTATGGTGTC TCGCTTGAGA TTATCATATA GAGATGAATT GCAGCTAGAG 
AACAGCATCC ACAAAGTGAA GGAAGTGGCT GCTAACTTTA TGCTGAAGTC ATATTCCATT 
CGGAACTTGA TTGATATCAA GAGTAACTCA GCAATCACCA AGCTGGTGCA GCAGACAGGC 
TTCTTGGGTC TTCAGCTTTC TGACAAGAAG AAGTTTTACA CGAAGACTCT GGTAGAGGAC 
ATGGCTATCT TTTGTAAGAG AAAGTATGGC AGGATTTCTT CATCTGGTGA TTTTGGAATA 
GTGAAGGGCT GCTTCTTTCA TGGGCTGGAT CCGTATGAAG AGATGGCTCA TTCAATTGCA 
GCTAGGGAGG TAATTGTTCG CTCTTCAAGG GGATTAGCTG AGCCTGGAAC CCTCTTTAAG 
AACCTGATGG CTGTTCTTCG GGATATCGTG ATTACAAACG ATGGAACTGT AAGAAACACC 
TGTAGTAACT CAGTCATCCA GTTCAAATAT GGAGTTGATT CTGAACGTGG ACATCAAGGT 
CTGTTTGAAG CTGGAGAACC TGTTGGAGTA TTGGCAGCTA CTGCCATGTC AAATCCTGCA 
TATAAGGCAG TGCTTGATTC CTCTCCAAAT AGTAATTCAT CATGGGAGCT AATGAAGGAA 
GTTCTGCTCT GCAAAGTCAA TTTCCAAAAC ACTACTAATG ATCGGCGCGT AATCCTTTAT 
TTAAACGAAT GCCATTGTGG AAAAAGGTTT TGCCAGGAAA ATGCTGCTTG CACAGTCAGG 
AACAAGCTGA ATAAAGTTAG TCTTAAAGAT ACTGCAGTGG AATTCCTAGT CGAATATAGA 
AAGCAACCAA CTATATCAGA AATTTTTGGA ATTGATTCAT GCCTACATGG CCATATTCAT 
CTTAATAAGA CATTGCTGCA AGATTGGAAC ATCAGTATGC AGGATATACA TCAAAAGTGT 
GAGGATGTAA TTAACTCACT AGGACAAAAG AAAAAGAAGA AAGCTACTGA TGATTTCAAG 
AGAACAAGTT TGTCTGTCAG TGAGTGCTGC TCTTTCCGAG ATCCTTGTGG GAGCAAAGGT 
TCGGATATGC CTTGTTTGAC GTTCTCCTAC AATGCCACCG ATCCCGATCT AGAACGAACT 
CTTGATGTTC TTTGCAATAC AGTTTACCCA GTTCTGCTGG AAATAGTGAT CAAAGGTGAT 
TCACGGATTT GCTCAGCAAA TATAATCTGG AATAGTTCAG ACATGACAAC GTGGATCCGG 
AATCGTCATG CATCCCGCCG AGGGGAATGG GTTTTGGATG TCACTGTTGA AAAATCTGCT 
GTCAAGCAAA GTGGTGATGC ATGGAGGGTT GTGATAGACT CATGTCTTTC AGTGCTTCAT 
CTAATAGACA CAAAACGGTC AATCCCTTAT TCTGTAAAAC AAGTGCAAGA ATTGCTTGGG 
TTATCCTGTG CTTTTGAGCA AGCAGTACAG CGTCTATCAG CTTCAGTGAG AATGGTCTCC 
AAGGGAGTTT TGAAAGAACA CATCATTCTC CTAGCAAACA ACATGACTTG TTCAGGGACT 
ATGTTGGGTT TCAATTCTGG AGGTTACAAA GCTTTGACCC GATCCTTAAA CATCAAGGCT 
CCATTCACAG AAGCAACTCT GATTGCACCA AGAAAATGTT TTGAGAAAGC GGCTGAGAAA 
TGTCATACAG ATTCACTATC AACAGTCGTT GGGTCTTGTT CTTGGGGAAA ACGCGTGGAT 
GTTGGTACAG GCTCACAGTT TGAGCTCCTA TGGAACCAAA AAGAGACTGG TCTAGATGAT 
AAAGAAGAAA CAGATGTGTA CAGCTTCCTT CAAATGGTCA TATCCACGAC AAACGCAGAT 
GCATTTGTTT CTTCCCCTGG TTTTGATGTT ACAGAGGAAG AAATGGCTGA ATGGGCTGAA 
TCGCCCGAGC GAGACTCTGC TCTTGGAGAG CCCAAGTTTG AGGACAGTGC CGACTTCCAA 
AATTTACATG ACGAAGGCAA GCCATCAGGA GCCAACTGGG AGAAAAGCTC TTCGTGGGAC 
AATGGTTGCA GTGGTGGTTC AGAGTGGGGA GTCTCAAAAA GTACAGGTGG TGAGGCAAAC 
CCGGAGTCTA ATTGGGAAAA GACTACAAAT GTTGAGAAGG AAGATGCTTG GTCTAGTTGG 
AACACTAGAA AAGATGCTCA AGAATCTTCC AAGTCAGATT CAGGGGGTGC TTGGGGTATA 
AAGACAAAGG ATGCTGATGC AGATACAACA CCAAACTGGG AAACAAGTCC AGCTCCGAAG 
GATTCCATTG TCCCAGAAAA TAATGAGCCG ACTTCTGACG TTTGGGGACA CAAATCTGTT 
TCAGACAAAT CGTGGGACAA AAAGAACTGG GGAACTGAAT CAGCTCCTGC TGCATGGGGT 
TCTACAGATG CTGCTGTTTG GGGTTCTAGT GATAAAAAGA ACTCGGAAAC AGAATCAGAT 
GCTGCTGCTT GGGGTTCCAG GGACAAAAAT AATTCTGACG TTGGGTCAGG TGCTGGTGTT 
TTAGGTCCCT GGAACAAGAA GAGCTCGGAA ACTGAGTCAA ATGGTGCTAC TTGGGGTTCT 
AGTGACAAAA CCAAATCAGG TGCTGCTGCA TGGAATTCTT GGGACAAAAA GAACATAGAA 
ACTGACTCAG AACCTGCTGC TTGGGGTTCT CAGGGAAAAA AGAATTCTGA AACTGAGTCA 
GGTCCTGCTG CTTGGGGTGC TTGGGACAAA AAGAAATCGG AAACTGAACC AGGTCCTGCT 
GGTTGGGGTA TGGGGGACAA AAAGAATTCT GAAACTGAAT TAGGCCCTGC TGCTATGGGT 
AATTGGGACA AAAAGAAATC GGATACGAAA TCAGGTCCAG CTGCTTGGGG ATCTACAGAT 
GCTGCTGCTT GGGGTTCTAG TGACAAAAAC AACTCGGAAA CTGAATCAGA TGCTGCTGCT 
TGGGGTTCTA GGAACAAAAA GACTTCTGAA ATTGAATCAG GTGCTGGCGC TTGGGGTTCG 
TGGGGACAGC CAAGTCCCAC TGCAGAAGAC AAGGATACCA ATGAGGATGA TAGAAATCCG 
TGGGTTTCCT TGAAGGAGAC CAAAAGTAGA GAGAAGGATG ATAAAGAGAG AAGCCAGTGG 
GGAAATCCAG CCAAGAAATT TCCATCTAGT GGTGGCTGGT CCAATGGTGG TGGTGCAGAC 
TGGAAAGGAA ATAGGAACCA TACTCCTAGG CCACCTAGAT CTGAGGATAA TTTGGCTCCC 
ATGTTTACAG CAACGAGACA GCGCTTGGAC TCATTCACCT CTGAGGAGCA AGAGCTGCTC 
TCAGATGTTG AGCCAGTCAT GAGGACTCTC AGGAAAATTA TGCATCCGTC TGCGTACCCT 
GATGGAGACC CAATTTCTGA TGATGACAAG ACGTTTGTCC TGGAGAAGAT TTTGAACTTC 
CATCCCCAAA AAGAGACAAA GCTTGGTTCT GGCGTTGACT TCATCACGGT TGATAAGCAC 
ACCATCTTCT CAGACAGCAG GTGCTTCTTT GTTGTCTCAA CTGATGGGGC CAAACAGGAC 
TTTTCATACC GAAAATCCCT CAACAACTAT TTGATGAAGA AATACCCTGA CCGTGCTGAA 
GAATTCATTG ACAAGTACTT TACGAAACCT CGGCCTAGCG GAAACAGAGA CAGGAACAAC 
CAAGATGCCA CACCACCTGG GGAAGAACAG TCTCAGCCTC CGAATCAGTC TATAGGTAAT 
GGAGGTGACG ACTTTCAGAC ACAGACTCAG TCTCAGTCTC CATCTCAGAC TCGGGCTCAG 
TCTCCATCTC AGGCTCAGGC TCAGTCTCCA TCTCAGACGC AGTCTCAGTC TCAGTCTCAG 
TCTCAGTCTC AGTCTCAGTC TCAGTCTCAG TCTCAGTCTC AGTCTCAGTC TCAGTCTCAG 
TCTCAGTCTC AGTCTCAGTC TCCATCCCAG ACTCAGACTC AGTCTCCGTC TCAGACTCAG 
GCTCAGGCTC AGTCTCCATC ATCTCAGTCT CCGTCCCAGA CGCAGACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRPD1B ( NM_001336818.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001323985.1 Arabidopsis thaliana nuclear RNA polymerase D1B (NRPD1B), mRNA.

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CloneID	OAb36467
Clone ID Related Accession (Same CDS sequence)	NM_001336818.1
Accession Version	NM_001336818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	nuclear RNA polymerase D1B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071).

ATGGAGGAAG AATCTACATC AGAGATTCTT GACGGAGAGA TTGTTGGAAT CACATTTGCT 
CTTGCTAGTC ACCATGAAAT ATGTATTCAA TCGATTAGTG AATCCGCAAT TAATCATCCC 
AGTCAACTCA CTAACGCATT CTTGGGGTTA CCTCTTGAAT TTGGTAAATG TGAATCTTGC 
GGTGCGACAG AACCAGATAA ATGTGAAGGT CATTTTGGTT ATATTCAGCT TCCAGTGCCA 
ATATACCATC CTGCTCATGT TAATGAGTTG AAACAAATGC TGAGCCTTCT GTGCTTGAAA 
TGTCTGAAGA TAAAGAAGGC AAAGGGCACA AGTGGTGGTC TGGCGGATCG CTTGTTGGGT 
GTCTGTTGTG AGGAAGCTTC GCAAATCTCA ATCAAAGATA GAGCATCAGA TGGTGCCTCA 
TATCTAGAAC TGAAGCTCCC ATCTAGGTCA AGACTGCAGC CTGGTTGCTG GAATTTCCTG 
GAAAGATATG GTTATCGCTA TGGAAGCGAT TACACGCGTC CCCTGCTGGC AAGAGAGGTT 
AAGGAAATTC TAAGACGGAT CCCCGAAGAA TCAAGAAAGA AACTCACGGC AAAGGGACAT 
ATTCCTCAAG AAGGATACAT ACTAGAGTAT CTTCCTGTCC CGCCTAACTG TTTGTCAGTG 
CCAGAGGCTT CTGATGGTTT CAGCACCATG TCAGTGGATC CTTCCAGAAT TGAGCTAAAG 
GACGTTCTGA AGAAAGTCAT AGCCATAAAA AGCTCTAGGT CTGGCGAGAC AAACTTTGAG 
TCGCATAAGG CTGAAGCCAG TGAGATGTTT AGAGTGGTGG ATACCTATCT CCAGGTGAGG 
GGTACTGCAA AAGCGGCAAG AAACATTGAT ATGAGATACG GGGTGTCTAA GATCTCGGAT 
AGTTCATCTT CAAAAGCTTG GACAGAAAAA ATGAGAACAC TTTTCATCCG TAAGGGTTCA 
GGTTTCTCTT CCCGTAGCGT CATCACTGGA GATGCTTACC GACATGTGAA TGAGGTAGGG 
ATCCCAATAG AAATTGCGCA GAGAATCACA TTTGAGGAAA GGGTTAGTGT CCACAATAGA 
GGTTATCTAC AAAAGTTGGT AGACGACAAG TTGTGTTTGA GTTATACTCA AGGTTCTACG 
ACCTATTCTT TAAGGGATGG ATCCAAGGGG CACACTGAGC TTAAACCCGG GCAGGTTGTT 
CATCGCAGGG TCATGGATGG GGATGTTGTG TTTATCAACA GACCCCCGAC AACACATAAA 
CATTCTCTTC AAGCGCTTCG GGTGTATGTT CATGAAGACA ACACCGTGAA GATTAACCCT 
CTGATGTGTA GCCCCCTCAG TGCTGATTTT GATGGTGATT GTGTCCATTT GTTCTACCCT 
CAGTCTCTTA GTGCTAAGGC AGAGGTTATG GAGCTCTTCT CTGTGGAGAA GCAACTGCTT 
AGCTCACATA CTGGACAGCT GATTTTGCAG ATGGGTAGTG ATTCTTTACT GTCTTTGAGG 
GTAATGCTGG AGCGGGTATT TCTGGACAAG GCAACTGCTC AACAGTTAGC TATGTATGGC 
TCTCTGTCCC TGCCACCACC AGCACTAAGG AAGTCCAGTA AATCTGGCCC AGCTTGGACA 
GTTTTCCAGA TTCTACAGCT TGCCTTTCCA GAACGTCTGA GTTGCAAAGG AGACAGATTC 
CTGGTTGATG GAAGTGATTT GCTGAAGTTT GATTTCGGGG TTGACGCGAT GGGGTCCATC 
ATCAATGAAA TTGTAACTTC AATCTTCCTG GAGAAGGGTC CAAAGGAGAC ACTTGGATTC 
TTTGATTCGC TGCAGCCTCT ATTGATGGAA AGTCTGTTTG CTGAGGGATT TAGTCTAAGT 
TTGGAAGACT TATCCATGTC TAGGGCAGAC ATGGATGTAA TTCACAACCT AATAATCCGA 
GAGATTTCAC CTATGGTGTC TCGCTTGAGA TTATCATATA GAGATGAATT GCAGCTAGAG 
AACAGCATCC ACAAAGTGAA GGAAGTGGCT GCTAACTTTA TGCTGAAGTC ATATTCCATT 
CGGAACTTGA TTGATATCAA GAGTAACTCA GCAATCACCA AGCTGGTGCA GCAGACAGGC 
TTCTTGGGTC TTCAGCTTTC TGACAAGAAG AAGTTTTACA CGAAGACTCT GGTAGAGGAC 
ATGGCTATCT TTTGTAAGAG AAAGTATGGC AGGATTTCTT CATCTGGTGA TTTTGGAATA 
GTGAAGGGCT GCTTCTTTCA TGGGCTGGAT CCGTATGAAG AGATGGCTCA TTCAATTGCA 
GCTAGGGAGG TAATTGTTCG CTCTTCAAGG GGATTAGCTG AGCCTGGAAC CCTCTTTAAG 
AACCTGATGG CTGTTCTTCG GGATATCGTG ATTACAAACG ATGGAACTGT AAGAAACACC 
TGTAGTAACT CAGTCATCCA GTTCAAATAT GGAGTTGATT CTGAACGTGG ACATCAAGGT 
CTGTTTGAAG CTGGAGAACC TGTTGGAGTA TTGGCAGCTA CTGCCATGTC AAATCCTGCA 
TATAAGGCAG TGCTTGATTC CTCTCCAAAT AGTAATTCAT CATGGGAGCT AATGAAGGAA 
GTTCTGCTCT GCAAAGTCAA TTTCCAAAAC ACTACTAATG ATCGGCGCGT AATCCTTTAT 
TTAAACGAAT GCCATTGTGG AAAAAGGTTT TGCCAGGAAA ATGCTGCTTG CACAGTCAGG 
AACAAGCTGA ATAAAGTTAG TCTTAAAGAT ACTGCAGTGG AATTCCTAGT CGAATATAGA 
AAGCAACCAA CTATATCAGA AATTTTTGGA ATTGATTCAT GCCTACATGG CCATATTCAT 
CTTAATAAGA CATTGCTGCA AGATTGGAAC ATCAGTATGC AGGATATACA TCAAAAGTGT 
GAGGATGTAA TTAACTCACT AGGACAAAAG AAAAAGAAGA AAGCTACTGA TGATTTCAAG 
AGAACAAGTT TGTCTGTCAG TGAGTGCTGC TCTTTCCGAG ATCCTTGTGG GAGCAAAGGT 
TCGGATATGC CTTGTTTGAC GTTCTCCTAC AATGCCACCG ATCCCGATCT AGAACGAACT 
CTTGATGTTC TTTGCAATAC AGTTTACCCA GTTCTGCTGG AAATAGTGAT CAAAGGTGAT 
TCACGGATTT GCTCAGCAAA TATAATCTGG AATAGTTCAG ACATGACAAC GTGGATCCGG 
AATCGTCATG CATCCCGCCG AGGGGAATGG GTTTTGGATG TCACTGTTGA AAAATCTGCT 
GTCAAGCAAA GTGGTGATGC ATGGAGGGTT GTGATAGACT CATGTCTTTC AGTGCTTCAT 
CTAATAGACA CAAAACGGTC AATCCCTTAT TCTGTAAAAC AAGTGCAAGA ATTGCTTGGG 
TTATCCTGTG CTTTTGAGCA AGCAGTACAG CGTCTATCAG CTTCAGTGAG AATGGTCTCC 
AAGGGAGTTT TGAAAGAACA CATCATTCTC CTAGCAAACA ACATGACTTG TTCAGGGACT 
ATGTTGGGTT TCAATTCTGG AGGTTACAAA GCTTTGACCC GATCCTTAAA CATCAAGGCT 
CCATTCACAG AAGCAACTCT GATTGCACCA AGAAAATGTT TTGAGAAAGC GGCTGAGAAA 
TGTCATACAG ATTCACTATC AACAGTCGTT GGGTCTTGTT CTTGGGGAAA ACGCGTGGAT 
GTTGGTACAG GCTCACAGTT TGAGCTCCTA TGGAACCAAA AAGAGACTGG TCTAGATGAT 
AAAGAAGAAA CAGATGTGTA CAGCTTCCTT CAAATGGTCA TATCCACGAC AAACGCAGAT 
GCATTTGTTT CTTCCCCTGG TTTTGATGTT ACAGAGGAAG AAATGGCTGA ATGGGCTGAA 
TCGCCCGAGC GAGACTCTGC TCTTGGAGAG CCCAAGTTTG AGGACAGTGC CGACTTCCAA 
AATTTACATG ACGAAGGCAA GCCATCAGGA GCCAACTGGG AGAAAAGCTC TTCGTGGGAC 
AATGGTTGCA GTGGTGGTTC AGAGTGGGGA GTCTCAAAAA GTACAGGTGG TGAGGCAAAC 
CCGGAGTCTA ATTGGGAAAA GACTACAAAT GTTGAGAAGG AAGATGCTTG GTCTAGTTGG 
AACACTAGAA AAGATGCTCA AGAATCTTCC AAGTCAGATT CAGGGGGTGC TTGGGGTATA 
AAGACAAAGG ATGCTGATGC AGATACAACA CCAAACTGGG AAACAAGTCC AGCTCCGAAG 
GATTCCATTG TCCCAGAAAA TAATGAGCCG ACTTCTGACG TTTGGGGACA CAAATCTGTT 
TCAGACAAAT CGTGGGACAA AAAGAACTGG GGAACTGAAT CAGCTCCTGC TGCATGGGGT 
TCTACAGATG CTGCTGTTTG GGGTTCTAGT GATAAAAAGA ACTCGGAAAC AGAATCAGAT 
GCTGCTGCTT GGGGTTCCAG GGACAAAAAT AATTCTGACG TTGGGTCAGG TGCTGGTGTT 
TTAGGTCCCT GGAACAAGAA GAGCTCGGAA ACTGAGTCAA ATGGTGCTAC TTGGGGTTCT 
AGTGACAAAA CCAAATCAGG TGCTGCTGCA TGGAATTCTT GGGACAAAAA GAACATAGAA 
ACTGACTCAG AACCTGCTGC TTGGGGTTCT CAGGGAAAAA AGAATTCTGA AACTGAGTCA 
GGTCCTGCTG CTTGGGGTGC TTGGGACAAA AAGAAATCGG AAACTGAACC AGGTCCTGCT 
GGTTGGGGTA TGGGGGACAA AAAGAATTCT GAAACTGAAT TAGGCCCTGC TGCTATGGGT 
AATTGGGACA AAAAGAAATC GGATACGAAA TCAGGTCCAG CTGCTTGGGG ATCTACAGAT 
GCTGCTGCTT GGGGTTCTAG TGACAAAAAC AACTCGGAAA CTGAATCAGA TGCTGCTGCT 
TGGGGTTCTA GGAACAAAAA GACTTCTGAA ATTGAATCAG GTGCTGGCGC TTGGGGTTCG 
TGGGGACAGC CAAGTCCCAC TGCAGAAGAC AAGGATACCA ATGAGGATGA TAGAAATCCG 
TGGGTTTCCT TGAAGGAGAC CAAAAGTAGA GAGAAGGATG ATAAAGAGAG AAGCCAGTGG 
GGAAATCCAG CCAAGAAATT TCCATCTAGT GGTGGCTGGT CCAATGGTGG TGGTGCAGAC 
TGGAAAGGAA ATAGGAACCA TACTCCTAGG CCACCTAGAT CTGAGGATAA TTTGGCTCCC 
ATGTTTACAG CAACGAGACA GCGCTTGGAC TCATTCACCT CTGAGGAGCA AGAGCTGCTC 
TCAGATGTTG AGCCAGTCAT GAGGACTCTC AGGAAAATTA TGCATCCGTC TGCGTATGCC 
TTTCTAGTTC TTACGCTCTG TTTATACAAT TTCCAGTTCT ATGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001323985.1
CDS	275..5602
Translation	MEEESTSEILDGEIVGITFALASHHEICIQSISESAINHPSQLTNAFLGLPLEFGKCESCGATEPDKCEGHFGYIQLPVPIYHPAHVNELKQMLSLLCLKCLKIKKAKGTSGGLADRLLGVCCEEASQISIKDRASDGASYLELKLPSRSRLQPGCWNFLERYGYRYGSDYTRPLLAREVKEILRRIPEESRKKLTAKGHIPQEGYILEYLPVPPNCLSVPEASDGFSTMSVDPSRIELKDVLKKVIAIKSSRSGETNFESHKAEASEMFRVVDTYLQVRGTAKAARNIDMRYGVSKISDSSSSKAWTEKMRTLFIRKGSGFSSRSVITGDAYRHVNEVGIPIEIAQRITFEERVSVHNRGYLQKLVDDKLCLSYTQGSTTYSLRDGSKGHTELKPGQVVHRRVMDGDVVFINRPPTTHKHSLQALRVYVHEDNTVKINPLMCSPLSADFDGDCVHLFYPQSLSAKAEVMELFSVEKQLLSSHTGQLILQMGSDSLLSLRVMLERVFLDKATAQQLAMYGSLSLPPPALRKSSKSGPAWTVFQILQLAFPERLSCKGDRFLVDGSDLLKFDFGVDAMGSIINEIVTSIFLEKGPKETLGFFDSLQPLLMESLFAEGFSLSLEDLSMSRADMDVIHNLIIREISPMVSRLRLSYRDELQLENSIHKVKEVAANFMLKSYSIRNLIDIKSNSAITKLVQQTGFLGLQLSDKKKFYTKTLVEDMAIFCKRKYGRISSSGDFGIVKGCFFHGLDPYEEMAHSIAAREVIVRSSRGLAEPGTLFKNLMAVLRDIVITNDGTVRNTCSNSVIQFKYGVDSERGHQGLFEAGEPVGVLAATAMSNPAYKAVLDSSPNSNSSWELMKEVLLCKVNFQNTTNDRRVILYLNECHCGKRFCQENAACTVRNKLNKVSLKDTAVEFLVEYRKQPTISEIFGIDSCLHGHIHLNKTLLQDWNISMQDIHQKCEDVINSLGQKKKKKATDDFKRTSLSVSECCSFRDPCGSKGSDMPCLTFSYNATDPDLERTLDVLCNTVYPVLLEIVIKGDSRICSANIIWNSSDMTTWIRNRHASRRGEWVLDVTVEKSAVKQSGDAWRVVIDSCLSVLHLIDTKRSIPYSVKQVQELLGLSCAFEQAVQRLSASVRMVSKGVLKEHIILLANNMTCSGTMLGFNSGGYKALTRSLNIKAPFTEATLIAPRKCFEKAAEKCHTDSLSTVVGSCSWGKRVDVGTGSQFELLWNQKETGLDDKEETDVYSFLQMVISTTNADAFVSSPGFDVTEEEMAEWAESPERDSALGEPKFEDSADFQNLHDEGKPSGANWEKSSSWDNGCSGGSEWGVSKSTGGEANPESNWEKTTNVEKEDAWSSWNTRKDAQESSKSDSGGAWGIKTKDADADTTPNWETSPAPKDSIVPENNEPTSDVWGHKSVSDKSWDKKNWGTESAPAAWGSTDAAVWGSSDKKNSETESDAAAWGSRDKNNSDVGSGAGVLGPWNKKSSETESNGATWGSSDKTKSGAAAWNSWDKKNIETDSEPAAWGSQGKKNSETESGPAAWGAWDKKKSETEPGPAGWGMGDKKNSETELGPAAMGNWDKKKSDTKSGPAAWGSTDAAAWGSSDKNNSETESDAAAWGSRNKKTSEIESGAGAWGSWGQPSPTAEDKDTNEDDRNPWVSLKETKSREKDDKERSQWGNPAKKFPSSGGWSNGGGADWKGNRNHTPRPPRSEDNLAPMFTATRQRLDSFTSEEQELLSDVEPVMRTLRKIMHPSAYAFLVLTLCLYNFQFYV

Target ORF information:

RefSeq Version	NM_001336818.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana nuclear RNA polymerase D1B (NRPD1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001336818.1

ORF Insert Sequence:

ATGGAGGAAG AATCTACATC AGAGATTCTT GACGGAGAGA TTGTTGGAAT CACATTTGCT 
CTTGCTAGTC ACCATGAAAT ATGTATTCAA TCGATTAGTG AATCCGCAAT TAATCATCCC 
AGTCAACTCA CTAACGCATT CTTGGGGTTA CCTCTTGAAT TTGGTAAATG TGAATCTTGC 
GGTGCGACAG AACCAGATAA ATGTGAAGGT CATTTTGGTT ATATTCAGCT TCCAGTGCCA 
ATATACCATC CTGCTCATGT TAATGAGTTG AAACAAATGC TGAGCCTTCT GTGCTTGAAA 
TGTCTGAAGA TAAAGAAGGC AAAGGGCACA AGTGGTGGTC TGGCGGATCG CTTGTTGGGT 
GTCTGTTGTG AGGAAGCTTC GCAAATCTCA ATCAAAGATA GAGCATCAGA TGGTGCCTCA 
TATCTAGAAC TGAAGCTCCC ATCTAGGTCA AGACTGCAGC CTGGTTGCTG GAATTTCCTG 
GAAAGATATG GTTATCGCTA TGGAAGCGAT TACACGCGTC CCCTGCTGGC AAGAGAGGTT 
AAGGAAATTC TAAGACGGAT CCCCGAAGAA TCAAGAAAGA AACTCACGGC AAAGGGACAT 
ATTCCTCAAG AAGGATACAT ACTAGAGTAT CTTCCTGTCC CGCCTAACTG TTTGTCAGTG 
CCAGAGGCTT CTGATGGTTT CAGCACCATG TCAGTGGATC CTTCCAGAAT TGAGCTAAAG 
GACGTTCTGA AGAAAGTCAT AGCCATAAAA AGCTCTAGGT CTGGCGAGAC AAACTTTGAG 
TCGCATAAGG CTGAAGCCAG TGAGATGTTT AGAGTGGTGG ATACCTATCT CCAGGTGAGG 
GGTACTGCAA AAGCGGCAAG AAACATTGAT ATGAGATACG GGGTGTCTAA GATCTCGGAT 
AGTTCATCTT CAAAAGCTTG GACAGAAAAA ATGAGAACAC TTTTCATCCG TAAGGGTTCA 
GGTTTCTCTT CCCGTAGCGT CATCACTGGA GATGCTTACC GACATGTGAA TGAGGTAGGG 
ATCCCAATAG AAATTGCGCA GAGAATCACA TTTGAGGAAA GGGTTAGTGT CCACAATAGA 
GGTTATCTAC AAAAGTTGGT AGACGACAAG TTGTGTTTGA GTTATACTCA AGGTTCTACG 
ACCTATTCTT TAAGGGATGG ATCCAAGGGG CACACTGAGC TTAAACCCGG GCAGGTTGTT 
CATCGCAGGG TCATGGATGG GGATGTTGTG TTTATCAACA GACCCCCGAC AACACATAAA 
CATTCTCTTC AAGCGCTTCG GGTGTATGTT CATGAAGACA ACACCGTGAA GATTAACCCT 
CTGATGTGTA GCCCCCTCAG TGCTGATTTT GATGGTGATT GTGTCCATTT GTTCTACCCT 
CAGTCTCTTA GTGCTAAGGC AGAGGTTATG GAGCTCTTCT CTGTGGAGAA GCAACTGCTT 
AGCTCACATA CTGGACAGCT GATTTTGCAG ATGGGTAGTG ATTCTTTACT GTCTTTGAGG 
GTAATGCTGG AGCGGGTATT TCTGGACAAG GCAACTGCTC AACAGTTAGC TATGTATGGC 
TCTCTGTCCC TGCCACCACC AGCACTAAGG AAGTCCAGTA AATCTGGCCC AGCTTGGACA 
GTTTTCCAGA TTCTACAGCT TGCCTTTCCA GAACGTCTGA GTTGCAAAGG AGACAGATTC 
CTGGTTGATG GAAGTGATTT GCTGAAGTTT GATTTCGGGG TTGACGCGAT GGGGTCCATC 
ATCAATGAAA TTGTAACTTC AATCTTCCTG GAGAAGGGTC CAAAGGAGAC ACTTGGATTC 
TTTGATTCGC TGCAGCCTCT ATTGATGGAA AGTCTGTTTG CTGAGGGATT TAGTCTAAGT 
TTGGAAGACT TATCCATGTC TAGGGCAGAC ATGGATGTAA TTCACAACCT AATAATCCGA 
GAGATTTCAC CTATGGTGTC TCGCTTGAGA TTATCATATA GAGATGAATT GCAGCTAGAG 
AACAGCATCC ACAAAGTGAA GGAAGTGGCT GCTAACTTTA TGCTGAAGTC ATATTCCATT 
CGGAACTTGA TTGATATCAA GAGTAACTCA GCAATCACCA AGCTGGTGCA GCAGACAGGC 
TTCTTGGGTC TTCAGCTTTC TGACAAGAAG AAGTTTTACA CGAAGACTCT GGTAGAGGAC 
ATGGCTATCT TTTGTAAGAG AAAGTATGGC AGGATTTCTT CATCTGGTGA TTTTGGAATA 
GTGAAGGGCT GCTTCTTTCA TGGGCTGGAT CCGTATGAAG AGATGGCTCA TTCAATTGCA 
GCTAGGGAGG TAATTGTTCG CTCTTCAAGG GGATTAGCTG AGCCTGGAAC CCTCTTTAAG 
AACCTGATGG CTGTTCTTCG GGATATCGTG ATTACAAACG ATGGAACTGT AAGAAACACC 
TGTAGTAACT CAGTCATCCA GTTCAAATAT GGAGTTGATT CTGAACGTGG ACATCAAGGT 
CTGTTTGAAG CTGGAGAACC TGTTGGAGTA TTGGCAGCTA CTGCCATGTC AAATCCTGCA 
TATAAGGCAG TGCTTGATTC CTCTCCAAAT AGTAATTCAT CATGGGAGCT AATGAAGGAA 
GTTCTGCTCT GCAAAGTCAA TTTCCAAAAC ACTACTAATG ATCGGCGCGT AATCCTTTAT 
TTAAACGAAT GCCATTGTGG AAAAAGGTTT TGCCAGGAAA ATGCTGCTTG CACAGTCAGG 
AACAAGCTGA ATAAAGTTAG TCTTAAAGAT ACTGCAGTGG AATTCCTAGT CGAATATAGA 
AAGCAACCAA CTATATCAGA AATTTTTGGA ATTGATTCAT GCCTACATGG CCATATTCAT 
CTTAATAAGA CATTGCTGCA AGATTGGAAC ATCAGTATGC AGGATATACA TCAAAAGTGT 
GAGGATGTAA TTAACTCACT AGGACAAAAG AAAAAGAAGA AAGCTACTGA TGATTTCAAG 
AGAACAAGTT TGTCTGTCAG TGAGTGCTGC TCTTTCCGAG ATCCTTGTGG GAGCAAAGGT 
TCGGATATGC CTTGTTTGAC GTTCTCCTAC AATGCCACCG ATCCCGATCT AGAACGAACT 
CTTGATGTTC TTTGCAATAC AGTTTACCCA GTTCTGCTGG AAATAGTGAT CAAAGGTGAT 
TCACGGATTT GCTCAGCAAA TATAATCTGG AATAGTTCAG ACATGACAAC GTGGATCCGG 
AATCGTCATG CATCCCGCCG AGGGGAATGG GTTTTGGATG TCACTGTTGA AAAATCTGCT 
GTCAAGCAAA GTGGTGATGC ATGGAGGGTT GTGATAGACT CATGTCTTTC AGTGCTTCAT 
CTAATAGACA CAAAACGGTC AATCCCTTAT TCTGTAAAAC AAGTGCAAGA ATTGCTTGGG 
TTATCCTGTG CTTTTGAGCA AGCAGTACAG CGTCTATCAG CTTCAGTGAG AATGGTCTCC 
AAGGGAGTTT TGAAAGAACA CATCATTCTC CTAGCAAACA ACATGACTTG TTCAGGGACT 
ATGTTGGGTT TCAATTCTGG AGGTTACAAA GCTTTGACCC GATCCTTAAA CATCAAGGCT 
CCATTCACAG AAGCAACTCT GATTGCACCA AGAAAATGTT TTGAGAAAGC GGCTGAGAAA 
TGTCATACAG ATTCACTATC AACAGTCGTT GGGTCTTGTT CTTGGGGAAA ACGCGTGGAT 
GTTGGTACAG GCTCACAGTT TGAGCTCCTA TGGAACCAAA AAGAGACTGG TCTAGATGAT 
AAAGAAGAAA CAGATGTGTA CAGCTTCCTT CAAATGGTCA TATCCACGAC AAACGCAGAT 
GCATTTGTTT CTTCCCCTGG TTTTGATGTT ACAGAGGAAG AAATGGCTGA ATGGGCTGAA 
TCGCCCGAGC GAGACTCTGC TCTTGGAGAG CCCAAGTTTG AGGACAGTGC CGACTTCCAA 
AATTTACATG ACGAAGGCAA GCCATCAGGA GCCAACTGGG AGAAAAGCTC TTCGTGGGAC 
AATGGTTGCA GTGGTGGTTC AGAGTGGGGA GTCTCAAAAA GTACAGGTGG TGAGGCAAAC 
CCGGAGTCTA ATTGGGAAAA GACTACAAAT GTTGAGAAGG AAGATGCTTG GTCTAGTTGG 
AACACTAGAA AAGATGCTCA AGAATCTTCC AAGTCAGATT CAGGGGGTGC TTGGGGTATA 
AAGACAAAGG ATGCTGATGC AGATACAACA CCAAACTGGG AAACAAGTCC AGCTCCGAAG 
GATTCCATTG TCCCAGAAAA TAATGAGCCG ACTTCTGACG TTTGGGGACA CAAATCTGTT 
TCAGACAAAT CGTGGGACAA AAAGAACTGG GGAACTGAAT CAGCTCCTGC TGCATGGGGT 
TCTACAGATG CTGCTGTTTG GGGTTCTAGT GATAAAAAGA ACTCGGAAAC AGAATCAGAT 
GCTGCTGCTT GGGGTTCCAG GGACAAAAAT AATTCTGACG TTGGGTCAGG TGCTGGTGTT 
TTAGGTCCCT GGAACAAGAA GAGCTCGGAA ACTGAGTCAA ATGGTGCTAC TTGGGGTTCT 
AGTGACAAAA CCAAATCAGG TGCTGCTGCA TGGAATTCTT GGGACAAAAA GAACATAGAA 
ACTGACTCAG AACCTGCTGC TTGGGGTTCT CAGGGAAAAA AGAATTCTGA AACTGAGTCA 
GGTCCTGCTG CTTGGGGTGC TTGGGACAAA AAGAAATCGG AAACTGAACC AGGTCCTGCT 
GGTTGGGGTA TGGGGGACAA AAAGAATTCT GAAACTGAAT TAGGCCCTGC TGCTATGGGT 
AATTGGGACA AAAAGAAATC GGATACGAAA TCAGGTCCAG CTGCTTGGGG ATCTACAGAT 
GCTGCTGCTT GGGGTTCTAG TGACAAAAAC AACTCGGAAA CTGAATCAGA TGCTGCTGCT 
TGGGGTTCTA GGAACAAAAA GACTTCTGAA ATTGAATCAG GTGCTGGCGC TTGGGGTTCG 
TGGGGACAGC CAAGTCCCAC TGCAGAAGAC AAGGATACCA ATGAGGATGA TAGAAATCCG 
TGGGTTTCCT TGAAGGAGAC CAAAAGTAGA GAGAAGGATG ATAAAGAGAG AAGCCAGTGG 
GGAAATCCAG CCAAGAAATT TCCATCTAGT GGTGGCTGGT CCAATGGTGG TGGTGCAGAC 
TGGAAAGGAA ATAGGAACCA TACTCCTAGG CCACCTAGAT CTGAGGATAA TTTGGCTCCC 
ATGTTTACAG CAACGAGACA GCGCTTGGAC TCATTCACCT CTGAGGAGCA AGAGCTGCTC 
TCAGATGTTG AGCCAGTCAT GAGGACTCTC AGGAAAATTA TGCATCCGTC TGCGTATGCC 
TTTCTAGTTC TTACGCTCTG TTTATACAAT TTCCAGTTCT ATGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Nature402(6763)761-8(1999 Dec)
Lin X,Kaul S,Rounsley S,Shea TP,Benito MI,Town CD,Fujii CY,Mason T,Bowman CL,Barnstead M,Feldblyum TV,Buell CR,Ketchum KA,Lee J,Ronning CM,Koo HL,Moffat KS,Cronin LA,Shen M,Pai G,Van Aken S,Umayam L,Tallon LJ,Gill JE,Adams MD,Carrera AJ,Creasy TH,Goodman HM,Somerville CR,Copenhaver GP,Preuss D,Nierman WC,White O,Eisen JA,Salzberg SL,Fraser CM,Venter JC

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NRPD1B cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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