MEE22 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	MEE22
Entrez Gene ID	818043
Full Name	maternal effect embryo arrest 22
Synonyms	EMB1611,EMBRYO DEFECTIVE 1611,F19I3.1,F19I3_1,MATERNAL EFFECT EMBRYO ARREST 22
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001336528.1	NP_001324226.1	maternal effect embryo arrest 22
NM_001202750.1	NP_001189679.1	maternal effect embryo arrest 22
NM_179906.3	NP_850237.1	maternal effect embryo arrest 22
NM_001336527.1	NP_001324225.1	maternal effect embryo arrest 22

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Lin X, Kaul S, Rounsley S, Shea TP, Benito MI, Town CD, Fujii CY, Mason T, Bowman CL, Barnstead M, Feldblyum TV, Buell CR, Ketchum KA, Lee J, Ronning CM, Koo HL, Moffat KS, Cronin LA, Shen M, Pai G, Van Aken S, Umayam L, Tallon LJ, Gill JE, Adams MD, Carrera AJ, Creasy TH, Goodman HM, Somerville CR, Copenhaver GP, Preuss D, Nierman WC, White O, Eisen JA, Salzberg SL, Fraser CM, Venter JC
Nature402(6763)761-8(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

EMB1611 is required for maintenance of the stem cell regulatory pathway in the shoot meristem.
Title: The essential gene EMB1611 maintains shoot apical meristem function during Arabidopsis development.

The following MEE22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MEE22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb08770	NM_179906.3 Latest version!	Arabidopsis thaliana maternal effect embryo arrest 22 (MEE22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OAb29710	NM_001202750.1 Latest version!	Arabidopsis thaliana maternal effect embryo arrest 22 (MEE22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OAb29710	NM_001336527.1 Latest version!	Arabidopsis thaliana maternal effect embryo arrest 22 (MEE22), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OAb29710	NM_001336528.1 Latest version!	Arabidopsis thaliana maternal effect embryo arrest 22 (MEE22), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MEE22 ( NM_179906.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_850237.1 Arabidopsis thaliana maternal effect embryo arrest 22 (MEE22), mRNA.

Price & Availability↑

CloneID	OAb08770
Clone ID Related Accession (Same CDS sequence)	NM_179906.3
Accession Version	NM_179906.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	maternal effect embryo arrest 22
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). On Sep 12, 2016 this sequence version replaced NM_179906.2.

ATGGCGACGA CCAATGCTCC ACCTGAGCTA GCTTCAGGAA ACCCTTGCTG CCTCGCTTGG 
CAAGGGAAGT ACATAGGAAT GAAGAAGAGG CGAGATGCTT TTAAAGAAGG AGTAACTCTT 
CTCCAGAAGG CTATAGAAAA TGTTAATGCT GAAAAATCCA ATCTCGAGAG AAAATTTGGG 
GAGATGGCGA CTGACGGAGA TACTAAGGAA AATGGTTCAA CTGTGAAAGC ATCTTTGGAG 
AAAGAAATAT CTCGTTTGAA GTTTGAGATA GTGTCTTTGC AACAAAAACT GGAGCGGAAC 
CTTAAAGAAA AAAGTGAAGA AACAAAGCTT CTTCAGGATC AAGCTTCTGG TCGAGAAAAG 
GAGATCAATG AACTGAGGGA TCTCCTTAAG AAAGAAACAT TAAGGGCAGA TAGTTCTGAA 
GAAGAGAGGG AACATGCTTT TAAGGAACTG AACAAGGCAA AGGCTCTGAT AGTAAAAGAT 
GAGGAAATCG AGCAAGATAT TCCTGAAGTA AAAAGGGAAA TAAGTCTGGT TAAAAATCTT 
CTTGCTAGTG AGAGACAGAA GACAGAGTCA GAGAGGAAGA AAGCAGAATC AGAGAAAAAG 
AAAGCTGATA AGTATCTTTC GGAGTTGGAG GTGTTAAGAA ATTCAGCTCA TAAGACTAGT 
TCTGATTTAC TTACTTTGAC CTCCAATCTT GAGACTGTAA AAAAGCAGCT TGAGCTTGAA 
AAACAGAAGA CACTGAAAGA GAAAAAACGT GCAGATATGG AGAGTGCAAA AGCTAGGGAC 
CAGATGAAGC TGGCAGAAGA TGTGTCTAAG AAGTTTGAAA TCGTTAGAGC AAGAAATGAA 
GAGCTCAAGA AAGAAATGGA ATCGCAGACC GCTAGTAGCC AAGTGAAGTT TGCTGAAAAC 
TCGGAAAAGC TGGAAGAGAA GATAAGGCTC CTTGAGATGA ATAAAAAAAC AGCCATGGAC 
TGGAAATCTC GTACTGATGA TCTGACTCAA CAGTTACAAG AAGCGCAATT AGTCGCTGAA 
GGTTTGAAGA AACAAGTGCA TGAGCTTTCA CTTTCCCAGA AATCCATCAA GACTCACTCT 
ATTTCTCCTC AGAAAGTCAG AGACCTGGAA AAGGCTGAAA TGAGGCTTTT GAAAAAAAAG 
ATGAAGTTTG AGAGAAATTG TGCAAAACAC TCCCAAACAG TGGCTAAATT TGAAAAGTTT 
CGAAGGGAAT TTCAGTGCGA AGAGCTAGGT CGGTTGAAAC TGGAGTTCGG TAGTCTTACG 
AATCGCATGA ATCTACTGGA TGAGTATTTT TCAACAGACG TTGAAGGTAC AGCTGGCCTT 
GGAAAGGCTA CAGGATGCAG GAAACTGCTG ACGCTAAATT CACAGAAGAA CCGTAATGGT 
GAAAAGCATT CTGACGCAAG ATGCAAATTG GTAGCCAGCT CAGGTTATCA GGAGCAGGCT 
TGCAAGTTGT CTGCCCACTT AATTTCTAAA TCTGGACGGG GCGTGAGTGA GTCTGTCTCA 
GGTACTATTT CTCAATTGGA GTCTCCTACT GGAGGCTCTA GAAAATTACC GAGTTCTGGA 
GTAATTTCCA GCGCAACATC TTTTTCTGAT GGGCAGTTAC TGGCATCACA GGGAAGAGAG 
CAGTTCTCTG TTACTACTTC AGCAGAAATA GCAAAAGATA AACCGAATAT CCAACCAACA 
AAATCAAGTA TGCTCCAAAA GATCTCCGAT ACCAGTAAAA ATGGAAATCT CTGTTTGGTG 
GCAGAAAACT ATCTTCAAAG GTGTCAGAGA GATATTCATG AGAACTCTCG GAAAAGAAAA 
AGAATGTTAG AGGCAGTTGT GTCCCATAAG CATCTGGCAT CTGGTGATAA AAAGAAAAAT 
TTGCCGATTG GAGAGAAGAT GGGCACCTTG CAGTCCATGA TTGTAGGAAC TGGGTCTAGG 
CCTTCAGAAA AGGAAGAAAC CTTGGTCCCC CCTGATAGGC AAGGTGGCTC CTCCGCAATT 
GACATTACAG TTTCCAAGAA AAGAAGGGTT TCTTGTAAGA AGAAGATAAT TGTACAGAAT 
TCTCTTGAGT TCAACCAGTC AGGCAAGACT CCAGGTAATA TAGCTGGGAA AACTACGTGT 
CTCTCTACTG CTACTGGACA TGACGTGAAA ACATTATTTT CGGAGGACTT TGCTGCTACA 
GATTATATGA AGTTGCTAGA GTTGGATAAC TTGGAAGAAG AAAATTATTA CCAGATGGCT 
AGAGAATCGC TTCTGTCCCC TGATCTTCCT CAGGTAGATT TTTTGGGTTG TGAGATCATG 
AATGAGGACA AAAATCCTGC TAGGGCTATT GACTTGGCTG CCAGTAATAG CATGTATTTA 
CGTGAGACCA TACTTTCTAG TGAATCTCCT AGTCTCAACA CTCAGAATAT CTCAGTGACA 
GTTGAAATGC CACCCATGTT GAAACCGTTG CATGGGCATC TTCTTAAACA CTTTATTGTG 
TTTTCAAATA TTGAAGACCA GAACAGTATC ATCATAATAA TTCATGCTAC TAACAATTGT 
CTACAGCGTT GCCCGTCAGT TACTAAAGAA CAGTGGGCAG TGCCAGCGAT TTTGTCTTCT 
TTGAAAATGG AAGAAAACCT TTTGGCCCAG GAAAGGGCCT GTGTGTTCCT CTCCTTGTTG 
CTGCATAACT TCTCCATGGT TCACACAACA AAAACTGGGA ATACTCTGAA TGTTGATTCT 
TTCTCCTGCT TGGATTCTTT CTCAAAGCAT ATACGTGGTG TTATGGCTGA TACTGAAGCT 
GGAGTTATGC TTTCTGGATT TTCGGAAGAA CTCCTTTGTC TTCTTCAGGA CCTCCTTTCT 
GGGCAGCGGG TATTATTTTC GGTTAAATCC TCAGAAACAT GTGAATCTGA TTTAAGCATC 
CCTGTCACCC TGAATGGAGA AAATGTAGCT CTCGTCAACA AAATCGCTCT AACTGATCAA 
TTGGTGGCCG GAAGCGCTAT TTTGGCGGCA ATATGTACTG CACTTGATCG TATTGGATAT 
ATCTGCGAAG CTTCCTTTGA AATCCTGCAC AAGTACAGTC ATGAGAAAAC CTCAGTGCTA 
CTGACCATTC TTCACGTTTT TGCTTACATT GCTGGAGAGA AAATGGTGTT GTCTAGTGAG 
CATGGCATAT CAATTGCAGT GTTGAAATAC ATTGTCATGT TTCTAGAAAA CAAACATTTT 
GGTACTGTGG AGGGCAGTTC TCGATTGCAC CCAGGCAAGA ACAAGTGTCC ATTCTCAGAC 
AGGTCTTCCT CGCTGGAGGC TATGGCATCT AAGCTCATGG AAATTCTTCA GGAATTTACT 
GAGTCTAATA CTTTGCATAA AAGCTTGACT GGTTCATTGG GTTCTAGCCA CCTAGAGAAG 
ACCGAGTTTA GGCCGGCACA CAAAGATTTC CAGTGTGTAT TGACCAGGGA TCAAAGTATC 
AATCTCTGTG ACATTCTATC ATTGGTGGAG CTTATTGCTT GTTATACGGC TTGGGATTGG 
ACTAGTGCGA ACATTGTTGC TCCACTGCTT AAGATGCTGG GAATGCCATT GCCAATGAAC 
CTCTCTGTTG CAATCGTCTC CCTTCTTGGG CAACTTAGCA GTATTGGAGT GGATGCTGGT 
GGCTATGAAA ACGAAGGAAT CTCAAACTTG AGAGTGAAAC TGTCAGCATT TCTACAGTGT 
GAGACGACAC TAAAGGCCGG TTTTGCAGTG CAGATAGCAA CTGTGAGCTC CCTCCTGAAG 
ACGCTGCAGC TGAAATTCCC AATAGACTTT CAAGACAAAA CCACCATGAT TCCGGGTAGT 
GGCGACCAAA GCTTATCTGG TTCAGTCAAT GTGGTGACCA AGTGGTTGTC GTTGTTGAGC 
AAGGAACAAC GAGTTTTCGC ATTTGAGTTT CTACAAACCA ATGTTGTTAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_850237.1
CDS	198..4091
Translation	MATTNAPPELASGNPCCLAWQGKYIGMKKRRDAFKEGVTLLQKAIENVNAEKSNLERKFGEMATDGDTKENGSTVKASLEKEISRLKFEIVSLQQKLERNLKEKSEETKLLQDQASGREKEINELRDLLKKETLRADSSEEEREHAFKELNKAKALIVKDEEIEQDIPEVKREISLVKNLLASERQKTESERKKAESEKKKADKYLSELEVLRNSAHKTSSDLLTLTSNLETVKKQLELEKQKTLKEKKRADMESAKARDQMKLAEDVSKKFEIVRARNEELKKEMESQTASSQVKFAENSEKLEEKIRLLEMNKKTAMDWKSRTDDLTQQLQEAQLVAEGLKKQVHELSLSQKSIKTHSISPQKVRDLEKAEMRLLKKKMKFERNCAKHSQTVAKFEKFRREFQCEELGRLKLEFGSLTNRMNLLDEYFSTDVEGTAGLGKATGCRKLLTLNSQKNRNGEKHSDARCKLVASSGYQEQACKLSAHLISKSGRGVSESVSGTISQLESPTGGSRKLPSSGVISSATSFSDGQLLASQGREQFSVTTSAEIAKDKPNIQPTKSSMLQKISDTSKNGNLCLVAENYLQRCQRDIHENSRKRKRMLEAVVSHKHLASGDKKKNLPIGEKMGTLQSMIVGTGSRPSEKEETLVPPDRQGGSSAIDITVSKKRRVSCKKKIIVQNSLEFNQSGKTPGNIAGKTTCLSTATGHDVKTLFSEDFAATDYMKLLELDNLEEENYYQMARESLLSPDLPQVDFLGCEIMNEDKNPARAIDLAASNSMYLRETILSSESPSLNTQNISVTVEMPPMLKPLHGHLLKHFIVFSNIEDQNSIIIIIHATNNCLQRCPSVTKEQWAVPAILSSLKMEENLLAQERACVFLSLLLHNFSMVHTTKTGNTLNVDSFSCLDSFSKHIRGVMADTEAGVMLSGFSEELLCLLQDLLSGQRVLFSVKSSETCESDLSIPVTLNGENVALVNKIALTDQLVAGSAILAAICTALDRIGYICEASFEILHKYSHEKTSVLLTILHVFAYIAGEKMVLSSEHGISIAVLKYIVMFLENKHFGTVEGSSRLHPGKNKCPFSDRSSSLEAMASKLMEILQEFTESNTLHKSLTGSLGSSHLEKTEFRPAHKDFQCVLTRDQSINLCDILSLVELIACYTAWDWTSANIVAPLLKMLGMPLPMNLSVAIVSLLGQLSSIGVDAGGYENEGISNLRVKLSAFLQCETTLKAGFAVQIATVSSLLKTLQLKFPIDFQDKTTMIPGSGDQSLSGSVNVVTKWLSLLSKEQRVFAFEFLQTNVVR

Target ORF information:

RefSeq Version	NM_179906.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana maternal effect embryo arrest 22 (MEE22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_179906.3

ORF Insert Sequence:

ATGGCGACGA CCAATGCTCC ACCTGAGCTA GCTTCAGGAA ACCCTTGCTG CCTCGCTTGG 
CAAGGGAAGT ACATAGGAAT GAAGAAGAGG CGAGATGCTT TTAAAGAAGG AGTAACTCTT 
CTCCAGAAGG CTATAGAAAA TGTTAATGCT GAAAAATCCA ATCTCGAGAG AAAATTTGGG 
GAGATGGCGA CTGACGGAGA TACTAAGGAA AATGGTTCAA CTGTGAAAGC ATCTTTGGAG 
AAAGAAATAT CTCGTTTGAA GTTTGAGATA GTGTCTTTGC AACAAAAACT GGAGCGGAAC 
CTTAAAGAAA AAAGTGAAGA AACAAAGCTT CTTCAGGATC AAGCTTCTGG TCGAGAAAAG 
GAGATCAATG AACTGAGGGA TCTCCTTAAG AAAGAAACAT TAAGGGCAGA TAGTTCTGAA 
GAAGAGAGGG AACATGCTTT TAAGGAACTG AACAAGGCAA AGGCTCTGAT AGTAAAAGAT 
GAGGAAATCG AGCAAGATAT TCCTGAAGTA AAAAGGGAAA TAAGTCTGGT TAAAAATCTT 
CTTGCTAGTG AGAGACAGAA GACAGAGTCA GAGAGGAAGA AAGCAGAATC AGAGAAAAAG 
AAAGCTGATA AGTATCTTTC GGAGTTGGAG GTGTTAAGAA ATTCAGCTCA TAAGACTAGT 
TCTGATTTAC TTACTTTGAC CTCCAATCTT GAGACTGTAA AAAAGCAGCT TGAGCTTGAA 
AAACAGAAGA CACTGAAAGA GAAAAAACGT GCAGATATGG AGAGTGCAAA AGCTAGGGAC 
CAGATGAAGC TGGCAGAAGA TGTGTCTAAG AAGTTTGAAA TCGTTAGAGC AAGAAATGAA 
GAGCTCAAGA AAGAAATGGA ATCGCAGACC GCTAGTAGCC AAGTGAAGTT TGCTGAAAAC 
TCGGAAAAGC TGGAAGAGAA GATAAGGCTC CTTGAGATGA ATAAAAAAAC AGCCATGGAC 
TGGAAATCTC GTACTGATGA TCTGACTCAA CAGTTACAAG AAGCGCAATT AGTCGCTGAA 
GGTTTGAAGA AACAAGTGCA TGAGCTTTCA CTTTCCCAGA AATCCATCAA GACTCACTCT 
ATTTCTCCTC AGAAAGTCAG AGACCTGGAA AAGGCTGAAA TGAGGCTTTT GAAAAAAAAG 
ATGAAGTTTG AGAGAAATTG TGCAAAACAC TCCCAAACAG TGGCTAAATT TGAAAAGTTT 
CGAAGGGAAT TTCAGTGCGA AGAGCTAGGT CGGTTGAAAC TGGAGTTCGG TAGTCTTACG 
AATCGCATGA ATCTACTGGA TGAGTATTTT TCAACAGACG TTGAAGGTAC AGCTGGCCTT 
GGAAAGGCTA CAGGATGCAG GAAACTGCTG ACGCTAAATT CACAGAAGAA CCGTAATGGT 
GAAAAGCATT CTGACGCAAG ATGCAAATTG GTAGCCAGCT CAGGTTATCA GGAGCAGGCT 
TGCAAGTTGT CTGCCCACTT AATTTCTAAA TCTGGACGGG GCGTGAGTGA GTCTGTCTCA 
GGTACTATTT CTCAATTGGA GTCTCCTACT GGAGGCTCTA GAAAATTACC GAGTTCTGGA 
GTAATTTCCA GCGCAACATC TTTTTCTGAT GGGCAGTTAC TGGCATCACA GGGAAGAGAG 
CAGTTCTCTG TTACTACTTC AGCAGAAATA GCAAAAGATA AACCGAATAT CCAACCAACA 
AAATCAAGTA TGCTCCAAAA GATCTCCGAT ACCAGTAAAA ATGGAAATCT CTGTTTGGTG 
GCAGAAAACT ATCTTCAAAG GTGTCAGAGA GATATTCATG AGAACTCTCG GAAAAGAAAA 
AGAATGTTAG AGGCAGTTGT GTCCCATAAG CATCTGGCAT CTGGTGATAA AAAGAAAAAT 
TTGCCGATTG GAGAGAAGAT GGGCACCTTG CAGTCCATGA TTGTAGGAAC TGGGTCTAGG 
CCTTCAGAAA AGGAAGAAAC CTTGGTCCCC CCTGATAGGC AAGGTGGCTC CTCCGCAATT 
GACATTACAG TTTCCAAGAA AAGAAGGGTT TCTTGTAAGA AGAAGATAAT TGTACAGAAT 
TCTCTTGAGT TCAACCAGTC AGGCAAGACT CCAGGTAATA TAGCTGGGAA AACTACGTGT 
CTCTCTACTG CTACTGGACA TGACGTGAAA ACATTATTTT CGGAGGACTT TGCTGCTACA 
GATTATATGA AGTTGCTAGA GTTGGATAAC TTGGAAGAAG AAAATTATTA CCAGATGGCT 
AGAGAATCGC TTCTGTCCCC TGATCTTCCT CAGGTAGATT TTTTGGGTTG TGAGATCATG 
AATGAGGACA AAAATCCTGC TAGGGCTATT GACTTGGCTG CCAGTAATAG CATGTATTTA 
CGTGAGACCA TACTTTCTAG TGAATCTCCT AGTCTCAACA CTCAGAATAT CTCAGTGACA 
GTTGAAATGC CACCCATGTT GAAACCGTTG CATGGGCATC TTCTTAAACA CTTTATTGTG 
TTTTCAAATA TTGAAGACCA GAACAGTATC ATCATAATAA TTCATGCTAC TAACAATTGT 
CTACAGCGTT GCCCGTCAGT TACTAAAGAA CAGTGGGCAG TGCCAGCGAT TTTGTCTTCT 
TTGAAAATGG AAGAAAACCT TTTGGCCCAG GAAAGGGCCT GTGTGTTCCT CTCCTTGTTG 
CTGCATAACT TCTCCATGGT TCACACAACA AAAACTGGGA ATACTCTGAA TGTTGATTCT 
TTCTCCTGCT TGGATTCTTT CTCAAAGCAT ATACGTGGTG TTATGGCTGA TACTGAAGCT 
GGAGTTATGC TTTCTGGATT TTCGGAAGAA CTCCTTTGTC TTCTTCAGGA CCTCCTTTCT 
GGGCAGCGGG TATTATTTTC GGTTAAATCC TCAGAAACAT GTGAATCTGA TTTAAGCATC 
CCTGTCACCC TGAATGGAGA AAATGTAGCT CTCGTCAACA AAATCGCTCT AACTGATCAA 
TTGGTGGCCG GAAGCGCTAT TTTGGCGGCA ATATGTACTG CACTTGATCG TATTGGATAT 
ATCTGCGAAG CTTCCTTTGA AATCCTGCAC AAGTACAGTC ATGAGAAAAC CTCAGTGCTA 
CTGACCATTC TTCACGTTTT TGCTTACATT GCTGGAGAGA AAATGGTGTT GTCTAGTGAG 
CATGGCATAT CAATTGCAGT GTTGAAATAC ATTGTCATGT TTCTAGAAAA CAAACATTTT 
GGTACTGTGG AGGGCAGTTC TCGATTGCAC CCAGGCAAGA ACAAGTGTCC ATTCTCAGAC 
AGGTCTTCCT CGCTGGAGGC TATGGCATCT AAGCTCATGG AAATTCTTCA GGAATTTACT 
GAGTCTAATA CTTTGCATAA AAGCTTGACT GGTTCATTGG GTTCTAGCCA CCTAGAGAAG 
ACCGAGTTTA GGCCGGCACA CAAAGATTTC CAGTGTGTAT TGACCAGGGA TCAAAGTATC 
AATCTCTGTG ACATTCTATC ATTGGTGGAG CTTATTGCTT GTTATACGGC TTGGGATTGG 
ACTAGTGCGA ACATTGTTGC TCCACTGCTT AAGATGCTGG GAATGCCATT GCCAATGAAC 
CTCTCTGTTG CAATCGTCTC CCTTCTTGGG CAACTTAGCA GTATTGGAGT GGATGCTGGT 
GGCTATGAAA ACGAAGGAAT CTCAAACTTG AGAGTGAAAC TGTCAGCATT TCTACAGTGT 
GAGACGACAC TAAAGGCCGG TTTTGCAGTG CAGATAGCAA CTGTGAGCTC CCTCCTGAAG 
ACGCTGCAGC TGAAATTCCC AATAGACTTT CAAGACAAAA CCACCATGAT TCCGGGTAGT 
GGCGACCAAA GCTTATCTGG TTCAGTCAAT GTGGTGACCA AGTGGTTGTC GTTGTTGAGC 
AAGGAACAAC GAGTTTTCGC ATTTGAGTTT CTACAAACCA ATGTTGTTAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEE22 ( NM_001336528.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001324226.1 Arabidopsis thaliana maternal effect embryo arrest 22 (MEE22), partial mRNA.

Price & Availability↑

CloneID	OAb29710
Clone ID Related Accession (Same CDS sequence)	NM_001336528.1 , NM_001202750.1 , NM_001336527.1
Accession Version	NM_001336528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	maternal effect embryo arrest 22
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). COMPLETENESS: incomplete on the 3' end.

ATGGCGACTG ACGGAGATAC TAAGGAAAAT GGTTCAACTG TGAAAGCATC TTTGGAGAAA 
GAAATATCTC GTTTGAAGTT TGAGATAGTG TCTTTGCAAC AAAAACTGGA GCGGAACCTT 
AAAGAAAAAA GTGAAGAAAC AAAGCTTCTT CAGGATCAAG CTTCTGGTCG AGAAAAGGAG 
ATCAATGAAC TGAGGGATCT CCTTAAGAAA GAAACATTAA GGGCAGATAG TTCTGAAGAA 
GAGAGGGAAC ATGCTTTTAA GGAACTGAAC AAGGCAAAGG CTCTGATAGT AAAAGATGAG 
GAAATCGAGC AAGATATTCC TGAAGTAAAA AGGGAAATAA GTCTGGTTAA AAATCTTCTT 
GCTAGTGAGA GACAGAAGAC AGAGTCAGAG AGGAAGAAAG CAGAATCAGA GAAAAAGAAA 
GCTGATAAGT ATCTTTCGGA GTTGGAGGTG TTAAGAAATT CAGCTCATAA GACTAGTTCT 
GATTTACTTA CTTTGACCTC CAATCTTGAG ACTGTAAAAA AGCAGCTTGA GCTTGAAAAA 
CAGAAGACAC TGAAAGAGAA AAAACGTGCA GATATGGAGA GTGCAAAAGC TAGGGACCAG 
ATGAAGCTGG CAGAAGATGT GTCTAAGAAG TTTGAAATCG TTAGAGCAAG AAATGAAGAG 
CTCAAGAAAG AAATGGAATC GCAGACCGCT AGTAGCCAAG TGAAGTTTGC TGAAAACTCG 
GAAAAGCTGG AAGAGAAGAT AAGGCTCCTT GAGATGAATA AAAAAACAGC CATGGACTGG 
AAATCTCGTA CTGATGATCT GACTCAACAG TTACAAGAAG CGCAATTAGT CGCTGAAGGT 
TTGAAGAAAC AAGTGCATGA GCTTTCACTT TCCCAGAAAT CCATCAAGAC TCACTCTATT 
TCTCCTCAGA AAGTCAGAGA CCTGGAAAAG GCTGAAATGA GGCTTTTGAA AAAAAAGATG 
AAGTTTGAGA GAAATTGTGC AAAACACTCC CAAACAGTGG CTAAATTTGA AAAGTTTCGA 
AGGGAATTTC AGTGCGAAGA GCTAGGTCGG TTGAAACTGG AGTTCGGTAG TCTTACGAAT 
CGCATGAATC TACTGGATGA GTATTTTTCA ACAGACGTTG AAGGTACAGC TGGCCTTGGA 
AAGGCTACAG GATGCAGGAA ACTGCTGACG CTAAATTCAC AGAAGAACCG TAATGGTGAA 
AAGCATTCTG ACGCAAGATG CAAATTGGTA GCCAGCTCAG GTTATCAGGA GCAGGCTTGC 
AAGTTGTCTG CCCACTTAAT TTCTAAATCT GGACGGGGCG TGAGTGAGTC TGTCTCAGGT 
ACTATTTCTC AATTGGAGTC TCCTACTGGA GGCTCTAGAA AATTACCGAG TTCTGGAGTA 
ATTTCCAGCG CAACATCTTT TTCTGATGGG CAGTTACTGG CATCACAGGG AAGAGAGCAG 
TTCTCTGTTA CTACTTCAGC AGAAATAGCA AAAGATAAAC CGAATATCCA ACCAACAAAA 
TCAAGTATGC TCCAAAAGAT CTCCGATACC AGTAAAAATG GAAATCTCTG TTTGGTGGCA 
GAAAACTATC TTCAAAGGTG TCAGAGAGAT ATTCATGAGA ACTCTCGGAA AAGAAAAAGA 
ATGTTAGAGG CAGTTGTGTC CCATAAGCAT CTGGCATCTG GTGATAAAAA GAAAAATTTG 
CCGATTGGAG AGAAGATGGG CACCTTGCAG TCCATGATTG TAGGAACTGG GTCTAGGCCT 
TCAGAAAAGG AAGAAACCTT GGTCCCCCCT GATAGGCAAG GTGGCTCCTC CGCAATTGAC 
ATTACAGTTT CCAAGAAAAG AAGGGTTTCT TGTAAGAAGA AGATAATTGT ACAGAATTCT 
CTTGAGTTCA ACCAGTCAGG CAAGACTCCA GGTAATATAG CTGGGAAAAC TACGTGTCTC 
TCTACTGCTA CTGGACATGA CGTGAAAACA TTATTTTCGG AGGACTTTGC TGCTACAGAT 
TATATGAAGT TGCTAGAGTT GGATAACTTG GAAGAAGAAA ATTATTACCA GATGGCTAGA 
GAATCGCTTC TGTCCCCTGA TCTTCCTCAG GTAGATTTTT TGGGTTGTGA GATCATGAAT 
GAGGACAAAA ATCCTGCTAG GGCTATTGAC TTGGCTGCCA GTAATAGCAT GTATTTACGT 
GAGACCATAC TTTCTAGTGA ATCTCCTAGT CTCAACACTC AGAATATCTC AGTGACAGTT 
GAAATGCCAC CCATGTTGAA ACCGTTGCAT GGGCATCTTC TTAAACACTT TATTGTGTTT 
TCAAATATTG AAGACCAGAA CAGTATCATC ATAATAATTC ATGCTACTAA CAATTGTCTA 
CAGCGTTGCC CGTCAGTTAC TAAAGAACAG TGGGCAGTGC CAGCGATTTT GTCTTCTTTG 
AAAATGGAAG AAAACCTTTT GGCCCAGGAA AGGGCCTGTG TGTTCCTCTC CTTGTTGCTG 
CATAACTTCT CCATGGTTCA CACAACAAAA ACTGGGAATA CTCTGAATGT TGATTCTTTC 
TCCTGCTTGG ATTCTTTCTC AAAGCATATA CGTGGTGTTA TGGCTGATAC TGAAGCTGGA 
GTTATGCTTT CTGGATTTTC GGAAGAACTC CTTTGTCTTC TTCAGGACCT CCTTTCTGGG 
CAGCGGGTAT TATTTTCGGT TAAATCCTCA GAAACATGTG AATCTGATTT AAGCATCCCT 
GTCACCCTGA ATGGAGAAAA TGTAGCTCTC GTCAACAAAA TCGCTCTAAC TGATCAATTG 
GTGGCCGGAA GCGCTATTTT GGCGGCAATA TGTACTGCAC TTGATCGTAT TGGATATATC 
TGCGAAGCTT CCTTTGAAAT CCTGCACAAG TACAGTCATG AGAAAACCTC AGTGCTACTG 
ACCATTCTTC ACGTTTTTGC TTACATTGCT GGAGAGAAAA TGGTGTTGTC TAGTGAGCAT 
GGCATATCAA TTGCAGTGTT GAAATACATT GTCATGTTTC TAGAAAACAA ACATTTTGGT 
ACTGTGGAGG GCAGTTCTCG ATTGCACCCA GGCAAGAACA AGTGTCCATT CTCAGACAGG 
TCTTCCTCGC TGGAGGCTAT GGCATCTAAG CTCATGGAAA TTCTTCAGGA ATTTACTGAG 
TCTAATACTT TGCATAAAAG CTTGACTGGT TCATTGGGTT CTAGCCACCT AGAGAAGACC 
GAGTTTAGGC CGGCACACAA AGATTTCCAG TGTGTATTGA CCAGGGATCA AAGTATCAAT 
CTCTGTGACA TTCTATCATT GGTGGAGCTT ATTGCTTGTT ATACGGCTTG GGATTGGACT 
AGTGCGAACA TTGTTGCTCC ACTGCTTAAG ATGCTGGGAA TGCCATTGCC AATGAACCTC 
TCTGTTGCAA TCGTCTCCCT TCTTGGGCAA CTTAGCAGTA TTGGAGTGGA TGCTGGTGGC 
TATGAAAACG AAGGAATCTC AAACTTGAGA GTGAAACTGT CAGCATTTCT ACAGTGTGAG 
ACGACACTAA AGGCCGGTTT TGCAGTGCAG ATAGCAACTG TGAGCTCCCT CCTGAAGACG 
CTGCAGCTGA AATTCCCAAT AGACTTTCAA GACAAAACCA CCATGATTCC GGGTAGTGGC 
GACCAAAGCT TATCTGGTTC AGTCAATGTG GTGACCAAGT GGTTGTCGTT GTTGAGCAAG 
GAACAACGAG TTTTCGCATT TGAGTTTCTA CAAACCAATG TTGTTAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001324226.1
CDS	38..3748
Translation	MATDGDTKENGSTVKASLEKEISRLKFEIVSLQQKLERNLKEKSEETKLLQDQASGREKEINELRDLLKKETLRADSSEEEREHAFKELNKAKALIVKDEEIEQDIPEVKREISLVKNLLASERQKTESERKKAESEKKKADKYLSELEVLRNSAHKTSSDLLTLTSNLETVKKQLELEKQKTLKEKKRADMESAKARDQMKLAEDVSKKFEIVRARNEELKKEMESQTASSQVKFAENSEKLEEKIRLLEMNKKTAMDWKSRTDDLTQQLQEAQLVAEGLKKQVHELSLSQKSIKTHSISPQKVRDLEKAEMRLLKKKMKFERNCAKHSQTVAKFEKFRREFQCEELGRLKLEFGSLTNRMNLLDEYFSTDVEGTAGLGKATGCRKLLTLNSQKNRNGEKHSDARCKLVASSGYQEQACKLSAHLISKSGRGVSESVSGTISQLESPTGGSRKLPSSGVISSATSFSDGQLLASQGREQFSVTTSAEIAKDKPNIQPTKSSMLQKISDTSKNGNLCLVAENYLQRCQRDIHENSRKRKRMLEAVVSHKHLASGDKKKNLPIGEKMGTLQSMIVGTGSRPSEKEETLVPPDRQGGSSAIDITVSKKRRVSCKKKIIVQNSLEFNQSGKTPGNIAGKTTCLSTATGHDVKTLFSEDFAATDYMKLLELDNLEEENYYQMARESLLSPDLPQVDFLGCEIMNEDKNPARAIDLAASNSMYLRETILSSESPSLNTQNISVTVEMPPMLKPLHGHLLKHFIVFSNIEDQNSIIIIIHATNNCLQRCPSVTKEQWAVPAILSSLKMEENLLAQERACVFLSLLLHNFSMVHTTKTGNTLNVDSFSCLDSFSKHIRGVMADTEAGVMLSGFSEELLCLLQDLLSGQRVLFSVKSSETCESDLSIPVTLNGENVALVNKIALTDQLVAGSAILAAICTALDRIGYICEASFEILHKYSHEKTSVLLTILHVFAYIAGEKMVLSSEHGISIAVLKYIVMFLENKHFGTVEGSSRLHPGKNKCPFSDRSSSLEAMASKLMEILQEFTESNTLHKSLTGSLGSSHLEKTEFRPAHKDFQCVLTRDQSINLCDILSLVELIACYTAWDWTSANIVAPLLKMLGMPLPMNLSVAIVSLLGQLSSIGVDAGGYENEGISNLRVKLSAFLQCETTLKAGFAVQIATVSSLLKTLQLKFPIDFQDKTTMIPGSGDQSLSGSVNVVTKWLSLLSKEQRVFAFEFLQTNVVR

Target ORF information:

RefSeq Version	NM_001336528.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana maternal effect embryo arrest 22 (MEE22), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001336528.1

ORF Insert Sequence:

ATGGCGACTG ACGGAGATAC TAAGGAAAAT GGTTCAACTG TGAAAGCATC TTTGGAGAAA 
GAAATATCTC GTTTGAAGTT TGAGATAGTG TCTTTGCAAC AAAAACTGGA GCGGAACCTT 
AAAGAAAAAA GTGAAGAAAC AAAGCTTCTT CAGGATCAAG CTTCTGGTCG AGAAAAGGAG 
ATCAATGAAC TGAGGGATCT CCTTAAGAAA GAAACATTAA GGGCAGATAG TTCTGAAGAA 
GAGAGGGAAC ATGCTTTTAA GGAACTGAAC AAGGCAAAGG CTCTGATAGT AAAAGATGAG 
GAAATCGAGC AAGATATTCC TGAAGTAAAA AGGGAAATAA GTCTGGTTAA AAATCTTCTT 
GCTAGTGAGA GACAGAAGAC AGAGTCAGAG AGGAAGAAAG CAGAATCAGA GAAAAAGAAA 
GCTGATAAGT ATCTTTCGGA GTTGGAGGTG TTAAGAAATT CAGCTCATAA GACTAGTTCT 
GATTTACTTA CTTTGACCTC CAATCTTGAG ACTGTAAAAA AGCAGCTTGA GCTTGAAAAA 
CAGAAGACAC TGAAAGAGAA AAAACGTGCA GATATGGAGA GTGCAAAAGC TAGGGACCAG 
ATGAAGCTGG CAGAAGATGT GTCTAAGAAG TTTGAAATCG TTAGAGCAAG AAATGAAGAG 
CTCAAGAAAG AAATGGAATC GCAGACCGCT AGTAGCCAAG TGAAGTTTGC TGAAAACTCG 
GAAAAGCTGG AAGAGAAGAT AAGGCTCCTT GAGATGAATA AAAAAACAGC CATGGACTGG 
AAATCTCGTA CTGATGATCT GACTCAACAG TTACAAGAAG CGCAATTAGT CGCTGAAGGT 
TTGAAGAAAC AAGTGCATGA GCTTTCACTT TCCCAGAAAT CCATCAAGAC TCACTCTATT 
TCTCCTCAGA AAGTCAGAGA CCTGGAAAAG GCTGAAATGA GGCTTTTGAA AAAAAAGATG 
AAGTTTGAGA GAAATTGTGC AAAACACTCC CAAACAGTGG CTAAATTTGA AAAGTTTCGA 
AGGGAATTTC AGTGCGAAGA GCTAGGTCGG TTGAAACTGG AGTTCGGTAG TCTTACGAAT 
CGCATGAATC TACTGGATGA GTATTTTTCA ACAGACGTTG AAGGTACAGC TGGCCTTGGA 
AAGGCTACAG GATGCAGGAA ACTGCTGACG CTAAATTCAC AGAAGAACCG TAATGGTGAA 
AAGCATTCTG ACGCAAGATG CAAATTGGTA GCCAGCTCAG GTTATCAGGA GCAGGCTTGC 
AAGTTGTCTG CCCACTTAAT TTCTAAATCT GGACGGGGCG TGAGTGAGTC TGTCTCAGGT 
ACTATTTCTC AATTGGAGTC TCCTACTGGA GGCTCTAGAA AATTACCGAG TTCTGGAGTA 
ATTTCCAGCG CAACATCTTT TTCTGATGGG CAGTTACTGG CATCACAGGG AAGAGAGCAG 
TTCTCTGTTA CTACTTCAGC AGAAATAGCA AAAGATAAAC CGAATATCCA ACCAACAAAA 
TCAAGTATGC TCCAAAAGAT CTCCGATACC AGTAAAAATG GAAATCTCTG TTTGGTGGCA 
GAAAACTATC TTCAAAGGTG TCAGAGAGAT ATTCATGAGA ACTCTCGGAA AAGAAAAAGA 
ATGTTAGAGG CAGTTGTGTC CCATAAGCAT CTGGCATCTG GTGATAAAAA GAAAAATTTG 
CCGATTGGAG AGAAGATGGG CACCTTGCAG TCCATGATTG TAGGAACTGG GTCTAGGCCT 
TCAGAAAAGG AAGAAACCTT GGTCCCCCCT GATAGGCAAG GTGGCTCCTC CGCAATTGAC 
ATTACAGTTT CCAAGAAAAG AAGGGTTTCT TGTAAGAAGA AGATAATTGT ACAGAATTCT 
CTTGAGTTCA ACCAGTCAGG CAAGACTCCA GGTAATATAG CTGGGAAAAC TACGTGTCTC 
TCTACTGCTA CTGGACATGA CGTGAAAACA TTATTTTCGG AGGACTTTGC TGCTACAGAT 
TATATGAAGT TGCTAGAGTT GGATAACTTG GAAGAAGAAA ATTATTACCA GATGGCTAGA 
GAATCGCTTC TGTCCCCTGA TCTTCCTCAG GTAGATTTTT TGGGTTGTGA GATCATGAAT 
GAGGACAAAA ATCCTGCTAG GGCTATTGAC TTGGCTGCCA GTAATAGCAT GTATTTACGT 
GAGACCATAC TTTCTAGTGA ATCTCCTAGT CTCAACACTC AGAATATCTC AGTGACAGTT 
GAAATGCCAC CCATGTTGAA ACCGTTGCAT GGGCATCTTC TTAAACACTT TATTGTGTTT 
TCAAATATTG AAGACCAGAA CAGTATCATC ATAATAATTC ATGCTACTAA CAATTGTCTA 
CAGCGTTGCC CGTCAGTTAC TAAAGAACAG TGGGCAGTGC CAGCGATTTT GTCTTCTTTG 
AAAATGGAAG AAAACCTTTT GGCCCAGGAA AGGGCCTGTG TGTTCCTCTC CTTGTTGCTG 
CATAACTTCT CCATGGTTCA CACAACAAAA ACTGGGAATA CTCTGAATGT TGATTCTTTC 
TCCTGCTTGG ATTCTTTCTC AAAGCATATA CGTGGTGTTA TGGCTGATAC TGAAGCTGGA 
GTTATGCTTT CTGGATTTTC GGAAGAACTC CTTTGTCTTC TTCAGGACCT CCTTTCTGGG 
CAGCGGGTAT TATTTTCGGT TAAATCCTCA GAAACATGTG AATCTGATTT AAGCATCCCT 
GTCACCCTGA ATGGAGAAAA TGTAGCTCTC GTCAACAAAA TCGCTCTAAC TGATCAATTG 
GTGGCCGGAA GCGCTATTTT GGCGGCAATA TGTACTGCAC TTGATCGTAT TGGATATATC 
TGCGAAGCTT CCTTTGAAAT CCTGCACAAG TACAGTCATG AGAAAACCTC AGTGCTACTG 
ACCATTCTTC ACGTTTTTGC TTACATTGCT GGAGAGAAAA TGGTGTTGTC TAGTGAGCAT 
GGCATATCAA TTGCAGTGTT GAAATACATT GTCATGTTTC TAGAAAACAA ACATTTTGGT 
ACTGTGGAGG GCAGTTCTCG ATTGCACCCA GGCAAGAACA AGTGTCCATT CTCAGACAGG 
TCTTCCTCGC TGGAGGCTAT GGCATCTAAG CTCATGGAAA TTCTTCAGGA ATTTACTGAG 
TCTAATACTT TGCATAAAAG CTTGACTGGT TCATTGGGTT CTAGCCACCT AGAGAAGACC 
GAGTTTAGGC CGGCACACAA AGATTTCCAG TGTGTATTGA CCAGGGATCA AAGTATCAAT 
CTCTGTGACA TTCTATCATT GGTGGAGCTT ATTGCTTGTT ATACGGCTTG GGATTGGACT 
AGTGCGAACA TTGTTGCTCC ACTGCTTAAG ATGCTGGGAA TGCCATTGCC AATGAACCTC 
TCTGTTGCAA TCGTCTCCCT TCTTGGGCAA CTTAGCAGTA TTGGAGTGGA TGCTGGTGGC 
TATGAAAACG AAGGAATCTC AAACTTGAGA GTGAAACTGT CAGCATTTCT ACAGTGTGAG 
ACGACACTAA AGGCCGGTTT TGCAGTGCAG ATAGCAACTG TGAGCTCCCT CCTGAAGACG 
CTGCAGCTGA AATTCCCAAT AGACTTTCAA GACAAAACCA CCATGATTCC GGGTAGTGGC 
GACCAAAGCT TATCTGGTTC AGTCAATGTG GTGACCAAGT GGTTGTCGTT GTTGAGCAAG 
GAACAACGAG TTTTCGCATT TGAGTTTCTA CAAACCAATG TTGTTAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEE22 ( NM_001202750.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001189679.1 Arabidopsis thaliana maternal effect embryo arrest 22 (MEE22), mRNA.

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CloneID	OAb29710
Clone ID Related Accession (Same CDS sequence)	NM_001336528.1 , NM_001202750.1 , NM_001336527.1
Accession Version	NM_001202750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	maternal effect embryo arrest 22
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071).

ATGGCGACTG ACGGAGATAC TAAGGAAAAT GGTTCAACTG TGAAAGCATC TTTGGAGAAA 
GAAATATCTC GTTTGAAGTT TGAGATAGTG TCTTTGCAAC AAAAACTGGA GCGGAACCTT 
AAAGAAAAAA GTGAAGAAAC AAAGCTTCTT CAGGATCAAG CTTCTGGTCG AGAAAAGGAG 
ATCAATGAAC TGAGGGATCT CCTTAAGAAA GAAACATTAA GGGCAGATAG TTCTGAAGAA 
GAGAGGGAAC ATGCTTTTAA GGAACTGAAC AAGGCAAAGG CTCTGATAGT AAAAGATGAG 
GAAATCGAGC AAGATATTCC TGAAGTAAAA AGGGAAATAA GTCTGGTTAA AAATCTTCTT 
GCTAGTGAGA GACAGAAGAC AGAGTCAGAG AGGAAGAAAG CAGAATCAGA GAAAAAGAAA 
GCTGATAAGT ATCTTTCGGA GTTGGAGGTG TTAAGAAATT CAGCTCATAA GACTAGTTCT 
GATTTACTTA CTTTGACCTC CAATCTTGAG ACTGTAAAAA AGCAGCTTGA GCTTGAAAAA 
CAGAAGACAC TGAAAGAGAA AAAACGTGCA GATATGGAGA GTGCAAAAGC TAGGGACCAG 
ATGAAGCTGG CAGAAGATGT GTCTAAGAAG TTTGAAATCG TTAGAGCAAG AAATGAAGAG 
CTCAAGAAAG AAATGGAATC GCAGACCGCT AGTAGCCAAG TGAAGTTTGC TGAAAACTCG 
GAAAAGCTGG AAGAGAAGAT AAGGCTCCTT GAGATGAATA AAAAAACAGC CATGGACTGG 
AAATCTCGTA CTGATGATCT GACTCAACAG TTACAAGAAG CGCAATTAGT CGCTGAAGGT 
TTGAAGAAAC AAGTGCATGA GCTTTCACTT TCCCAGAAAT CCATCAAGAC TCACTCTATT 
TCTCCTCAGA AAGTCAGAGA CCTGGAAAAG GCTGAAATGA GGCTTTTGAA AAAAAAGATG 
AAGTTTGAGA GAAATTGTGC AAAACACTCC CAAACAGTGG CTAAATTTGA AAAGTTTCGA 
AGGGAATTTC AGTGCGAAGA GCTAGGTCGG TTGAAACTGG AGTTCGGTAG TCTTACGAAT 
CGCATGAATC TACTGGATGA GTATTTTTCA ACAGACGTTG AAGGTACAGC TGGCCTTGGA 
AAGGCTACAG GATGCAGGAA ACTGCTGACG CTAAATTCAC AGAAGAACCG TAATGGTGAA 
AAGCATTCTG ACGCAAGATG CAAATTGGTA GCCAGCTCAG GTTATCAGGA GCAGGCTTGC 
AAGTTGTCTG CCCACTTAAT TTCTAAATCT GGACGGGGCG TGAGTGAGTC TGTCTCAGGT 
ACTATTTCTC AATTGGAGTC TCCTACTGGA GGCTCTAGAA AATTACCGAG TTCTGGAGTA 
ATTTCCAGCG CAACATCTTT TTCTGATGGG CAGTTACTGG CATCACAGGG AAGAGAGCAG 
TTCTCTGTTA CTACTTCAGC AGAAATAGCA AAAGATAAAC CGAATATCCA ACCAACAAAA 
TCAAGTATGC TCCAAAAGAT CTCCGATACC AGTAAAAATG GAAATCTCTG TTTGGTGGCA 
GAAAACTATC TTCAAAGGTG TCAGAGAGAT ATTCATGAGA ACTCTCGGAA AAGAAAAAGA 
ATGTTAGAGG CAGTTGTGTC CCATAAGCAT CTGGCATCTG GTGATAAAAA GAAAAATTTG 
CCGATTGGAG AGAAGATGGG CACCTTGCAG TCCATGATTG TAGGAACTGG GTCTAGGCCT 
TCAGAAAAGG AAGAAACCTT GGTCCCCCCT GATAGGCAAG GTGGCTCCTC CGCAATTGAC 
ATTACAGTTT CCAAGAAAAG AAGGGTTTCT TGTAAGAAGA AGATAATTGT ACAGAATTCT 
CTTGAGTTCA ACCAGTCAGG CAAGACTCCA GGTAATATAG CTGGGAAAAC TACGTGTCTC 
TCTACTGCTA CTGGACATGA CGTGAAAACA TTATTTTCGG AGGACTTTGC TGCTACAGAT 
TATATGAAGT TGCTAGAGTT GGATAACTTG GAAGAAGAAA ATTATTACCA GATGGCTAGA 
GAATCGCTTC TGTCCCCTGA TCTTCCTCAG GTAGATTTTT TGGGTTGTGA GATCATGAAT 
GAGGACAAAA ATCCTGCTAG GGCTATTGAC TTGGCTGCCA GTAATAGCAT GTATTTACGT 
GAGACCATAC TTTCTAGTGA ATCTCCTAGT CTCAACACTC AGAATATCTC AGTGACAGTT 
GAAATGCCAC CCATGTTGAA ACCGTTGCAT GGGCATCTTC TTAAACACTT TATTGTGTTT 
TCAAATATTG AAGACCAGAA CAGTATCATC ATAATAATTC ATGCTACTAA CAATTGTCTA 
CAGCGTTGCC CGTCAGTTAC TAAAGAACAG TGGGCAGTGC CAGCGATTTT GTCTTCTTTG 
AAAATGGAAG AAAACCTTTT GGCCCAGGAA AGGGCCTGTG TGTTCCTCTC CTTGTTGCTG 
CATAACTTCT CCATGGTTCA CACAACAAAA ACTGGGAATA CTCTGAATGT TGATTCTTTC 
TCCTGCTTGG ATTCTTTCTC AAAGCATATA CGTGGTGTTA TGGCTGATAC TGAAGCTGGA 
GTTATGCTTT CTGGATTTTC GGAAGAACTC CTTTGTCTTC TTCAGGACCT CCTTTCTGGG 
CAGCGGGTAT TATTTTCGGT TAAATCCTCA GAAACATGTG AATCTGATTT AAGCATCCCT 
GTCACCCTGA ATGGAGAAAA TGTAGCTCTC GTCAACAAAA TCGCTCTAAC TGATCAATTG 
GTGGCCGGAA GCGCTATTTT GGCGGCAATA TGTACTGCAC TTGATCGTAT TGGATATATC 
TGCGAAGCTT CCTTTGAAAT CCTGCACAAG TACAGTCATG AGAAAACCTC AGTGCTACTG 
ACCATTCTTC ACGTTTTTGC TTACATTGCT GGAGAGAAAA TGGTGTTGTC TAGTGAGCAT 
GGCATATCAA TTGCAGTGTT GAAATACATT GTCATGTTTC TAGAAAACAA ACATTTTGGT 
ACTGTGGAGG GCAGTTCTCG ATTGCACCCA GGCAAGAACA AGTGTCCATT CTCAGACAGG 
TCTTCCTCGC TGGAGGCTAT GGCATCTAAG CTCATGGAAA TTCTTCAGGA ATTTACTGAG 
TCTAATACTT TGCATAAAAG CTTGACTGGT TCATTGGGTT CTAGCCACCT AGAGAAGACC 
GAGTTTAGGC CGGCACACAA AGATTTCCAG TGTGTATTGA CCAGGGATCA AAGTATCAAT 
CTCTGTGACA TTCTATCATT GGTGGAGCTT ATTGCTTGTT ATACGGCTTG GGATTGGACT 
AGTGCGAACA TTGTTGCTCC ACTGCTTAAG ATGCTGGGAA TGCCATTGCC AATGAACCTC 
TCTGTTGCAA TCGTCTCCCT TCTTGGGCAA CTTAGCAGTA TTGGAGTGGA TGCTGGTGGC 
TATGAAAACG AAGGAATCTC AAACTTGAGA GTGAAACTGT CAGCATTTCT ACAGTGTGAG 
ACGACACTAA AGGCCGGTTT TGCAGTGCAG ATAGCAACTG TGAGCTCCCT CCTGAAGACG 
CTGCAGCTGA AATTCCCAAT AGACTTTCAA GACAAAACCA CCATGATTCC GGGTAGTGGC 
GACCAAAGCT TATCTGGTTC AGTCAATGTG GTGACCAAGT GGTTGTCGTT GTTGAGCAAG 
GAACAACGAG TTTTCGCATT TGAGTTTCTA CAAACCAATG TTGTTAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189679.1
CDS	530..4240
Translation	MATDGDTKENGSTVKASLEKEISRLKFEIVSLQQKLERNLKEKSEETKLLQDQASGREKEINELRDLLKKETLRADSSEEEREHAFKELNKAKALIVKDEEIEQDIPEVKREISLVKNLLASERQKTESERKKAESEKKKADKYLSELEVLRNSAHKTSSDLLTLTSNLETVKKQLELEKQKTLKEKKRADMESAKARDQMKLAEDVSKKFEIVRARNEELKKEMESQTASSQVKFAENSEKLEEKIRLLEMNKKTAMDWKSRTDDLTQQLQEAQLVAEGLKKQVHELSLSQKSIKTHSISPQKVRDLEKAEMRLLKKKMKFERNCAKHSQTVAKFEKFRREFQCEELGRLKLEFGSLTNRMNLLDEYFSTDVEGTAGLGKATGCRKLLTLNSQKNRNGEKHSDARCKLVASSGYQEQACKLSAHLISKSGRGVSESVSGTISQLESPTGGSRKLPSSGVISSATSFSDGQLLASQGREQFSVTTSAEIAKDKPNIQPTKSSMLQKISDTSKNGNLCLVAENYLQRCQRDIHENSRKRKRMLEAVVSHKHLASGDKKKNLPIGEKMGTLQSMIVGTGSRPSEKEETLVPPDRQGGSSAIDITVSKKRRVSCKKKIIVQNSLEFNQSGKTPGNIAGKTTCLSTATGHDVKTLFSEDFAATDYMKLLELDNLEEENYYQMARESLLSPDLPQVDFLGCEIMNEDKNPARAIDLAASNSMYLRETILSSESPSLNTQNISVTVEMPPMLKPLHGHLLKHFIVFSNIEDQNSIIIIIHATNNCLQRCPSVTKEQWAVPAILSSLKMEENLLAQERACVFLSLLLHNFSMVHTTKTGNTLNVDSFSCLDSFSKHIRGVMADTEAGVMLSGFSEELLCLLQDLLSGQRVLFSVKSSETCESDLSIPVTLNGENVALVNKIALTDQLVAGSAILAAICTALDRIGYICEASFEILHKYSHEKTSVLLTILHVFAYIAGEKMVLSSEHGISIAVLKYIVMFLENKHFGTVEGSSRLHPGKNKCPFSDRSSSLEAMASKLMEILQEFTESNTLHKSLTGSLGSSHLEKTEFRPAHKDFQCVLTRDQSINLCDILSLVELIACYTAWDWTSANIVAPLLKMLGMPLPMNLSVAIVSLLGQLSSIGVDAGGYENEGISNLRVKLSAFLQCETTLKAGFAVQIATVSSLLKTLQLKFPIDFQDKTTMIPGSGDQSLSGSVNVVTKWLSLLSKEQRVFAFEFLQTNVVR

Target ORF information:

RefSeq Version	NM_001202750.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana maternal effect embryo arrest 22 (MEE22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202750.1

ORF Insert Sequence:

ATGGCGACTG ACGGAGATAC TAAGGAAAAT GGTTCAACTG TGAAAGCATC TTTGGAGAAA 
GAAATATCTC GTTTGAAGTT TGAGATAGTG TCTTTGCAAC AAAAACTGGA GCGGAACCTT 
AAAGAAAAAA GTGAAGAAAC AAAGCTTCTT CAGGATCAAG CTTCTGGTCG AGAAAAGGAG 
ATCAATGAAC TGAGGGATCT CCTTAAGAAA GAAACATTAA GGGCAGATAG TTCTGAAGAA 
GAGAGGGAAC ATGCTTTTAA GGAACTGAAC AAGGCAAAGG CTCTGATAGT AAAAGATGAG 
GAAATCGAGC AAGATATTCC TGAAGTAAAA AGGGAAATAA GTCTGGTTAA AAATCTTCTT 
GCTAGTGAGA GACAGAAGAC AGAGTCAGAG AGGAAGAAAG CAGAATCAGA GAAAAAGAAA 
GCTGATAAGT ATCTTTCGGA GTTGGAGGTG TTAAGAAATT CAGCTCATAA GACTAGTTCT 
GATTTACTTA CTTTGACCTC CAATCTTGAG ACTGTAAAAA AGCAGCTTGA GCTTGAAAAA 
CAGAAGACAC TGAAAGAGAA AAAACGTGCA GATATGGAGA GTGCAAAAGC TAGGGACCAG 
ATGAAGCTGG CAGAAGATGT GTCTAAGAAG TTTGAAATCG TTAGAGCAAG AAATGAAGAG 
CTCAAGAAAG AAATGGAATC GCAGACCGCT AGTAGCCAAG TGAAGTTTGC TGAAAACTCG 
GAAAAGCTGG AAGAGAAGAT AAGGCTCCTT GAGATGAATA AAAAAACAGC CATGGACTGG 
AAATCTCGTA CTGATGATCT GACTCAACAG TTACAAGAAG CGCAATTAGT CGCTGAAGGT 
TTGAAGAAAC AAGTGCATGA GCTTTCACTT TCCCAGAAAT CCATCAAGAC TCACTCTATT 
TCTCCTCAGA AAGTCAGAGA CCTGGAAAAG GCTGAAATGA GGCTTTTGAA AAAAAAGATG 
AAGTTTGAGA GAAATTGTGC AAAACACTCC CAAACAGTGG CTAAATTTGA AAAGTTTCGA 
AGGGAATTTC AGTGCGAAGA GCTAGGTCGG TTGAAACTGG AGTTCGGTAG TCTTACGAAT 
CGCATGAATC TACTGGATGA GTATTTTTCA ACAGACGTTG AAGGTACAGC TGGCCTTGGA 
AAGGCTACAG GATGCAGGAA ACTGCTGACG CTAAATTCAC AGAAGAACCG TAATGGTGAA 
AAGCATTCTG ACGCAAGATG CAAATTGGTA GCCAGCTCAG GTTATCAGGA GCAGGCTTGC 
AAGTTGTCTG CCCACTTAAT TTCTAAATCT GGACGGGGCG TGAGTGAGTC TGTCTCAGGT 
ACTATTTCTC AATTGGAGTC TCCTACTGGA GGCTCTAGAA AATTACCGAG TTCTGGAGTA 
ATTTCCAGCG CAACATCTTT TTCTGATGGG CAGTTACTGG CATCACAGGG AAGAGAGCAG 
TTCTCTGTTA CTACTTCAGC AGAAATAGCA AAAGATAAAC CGAATATCCA ACCAACAAAA 
TCAAGTATGC TCCAAAAGAT CTCCGATACC AGTAAAAATG GAAATCTCTG TTTGGTGGCA 
GAAAACTATC TTCAAAGGTG TCAGAGAGAT ATTCATGAGA ACTCTCGGAA AAGAAAAAGA 
ATGTTAGAGG CAGTTGTGTC CCATAAGCAT CTGGCATCTG GTGATAAAAA GAAAAATTTG 
CCGATTGGAG AGAAGATGGG CACCTTGCAG TCCATGATTG TAGGAACTGG GTCTAGGCCT 
TCAGAAAAGG AAGAAACCTT GGTCCCCCCT GATAGGCAAG GTGGCTCCTC CGCAATTGAC 
ATTACAGTTT CCAAGAAAAG AAGGGTTTCT TGTAAGAAGA AGATAATTGT ACAGAATTCT 
CTTGAGTTCA ACCAGTCAGG CAAGACTCCA GGTAATATAG CTGGGAAAAC TACGTGTCTC 
TCTACTGCTA CTGGACATGA CGTGAAAACA TTATTTTCGG AGGACTTTGC TGCTACAGAT 
TATATGAAGT TGCTAGAGTT GGATAACTTG GAAGAAGAAA ATTATTACCA GATGGCTAGA 
GAATCGCTTC TGTCCCCTGA TCTTCCTCAG GTAGATTTTT TGGGTTGTGA GATCATGAAT 
GAGGACAAAA ATCCTGCTAG GGCTATTGAC TTGGCTGCCA GTAATAGCAT GTATTTACGT 
GAGACCATAC TTTCTAGTGA ATCTCCTAGT CTCAACACTC AGAATATCTC AGTGACAGTT 
GAAATGCCAC CCATGTTGAA ACCGTTGCAT GGGCATCTTC TTAAACACTT TATTGTGTTT 
TCAAATATTG AAGACCAGAA CAGTATCATC ATAATAATTC ATGCTACTAA CAATTGTCTA 
CAGCGTTGCC CGTCAGTTAC TAAAGAACAG TGGGCAGTGC CAGCGATTTT GTCTTCTTTG 
AAAATGGAAG AAAACCTTTT GGCCCAGGAA AGGGCCTGTG TGTTCCTCTC CTTGTTGCTG 
CATAACTTCT CCATGGTTCA CACAACAAAA ACTGGGAATA CTCTGAATGT TGATTCTTTC 
TCCTGCTTGG ATTCTTTCTC AAAGCATATA CGTGGTGTTA TGGCTGATAC TGAAGCTGGA 
GTTATGCTTT CTGGATTTTC GGAAGAACTC CTTTGTCTTC TTCAGGACCT CCTTTCTGGG 
CAGCGGGTAT TATTTTCGGT TAAATCCTCA GAAACATGTG AATCTGATTT AAGCATCCCT 
GTCACCCTGA ATGGAGAAAA TGTAGCTCTC GTCAACAAAA TCGCTCTAAC TGATCAATTG 
GTGGCCGGAA GCGCTATTTT GGCGGCAATA TGTACTGCAC TTGATCGTAT TGGATATATC 
TGCGAAGCTT CCTTTGAAAT CCTGCACAAG TACAGTCATG AGAAAACCTC AGTGCTACTG 
ACCATTCTTC ACGTTTTTGC TTACATTGCT GGAGAGAAAA TGGTGTTGTC TAGTGAGCAT 
GGCATATCAA TTGCAGTGTT GAAATACATT GTCATGTTTC TAGAAAACAA ACATTTTGGT 
ACTGTGGAGG GCAGTTCTCG ATTGCACCCA GGCAAGAACA AGTGTCCATT CTCAGACAGG 
TCTTCCTCGC TGGAGGCTAT GGCATCTAAG CTCATGGAAA TTCTTCAGGA ATTTACTGAG 
TCTAATACTT TGCATAAAAG CTTGACTGGT TCATTGGGTT CTAGCCACCT AGAGAAGACC 
GAGTTTAGGC CGGCACACAA AGATTTCCAG TGTGTATTGA CCAGGGATCA AAGTATCAAT 
CTCTGTGACA TTCTATCATT GGTGGAGCTT ATTGCTTGTT ATACGGCTTG GGATTGGACT 
AGTGCGAACA TTGTTGCTCC ACTGCTTAAG ATGCTGGGAA TGCCATTGCC AATGAACCTC 
TCTGTTGCAA TCGTCTCCCT TCTTGGGCAA CTTAGCAGTA TTGGAGTGGA TGCTGGTGGC 
TATGAAAACG AAGGAATCTC AAACTTGAGA GTGAAACTGT CAGCATTTCT ACAGTGTGAG 
ACGACACTAA AGGCCGGTTT TGCAGTGCAG ATAGCAACTG TGAGCTCCCT CCTGAAGACG 
CTGCAGCTGA AATTCCCAAT AGACTTTCAA GACAAAACCA CCATGATTCC GGGTAGTGGC 
GACCAAAGCT TATCTGGTTC AGTCAATGTG GTGACCAAGT GGTTGTCGTT GTTGAGCAAG 
GAACAACGAG TTTTCGCATT TGAGTTTCTA CAAACCAATG TTGTTAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEE22 ( NM_001336527.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001324225.1 Arabidopsis thaliana maternal effect embryo arrest 22 (MEE22), partial mRNA.

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CloneID	OAb29710
Clone ID Related Accession (Same CDS sequence)	NM_001336528.1 , NM_001202750.1 , NM_001336527.1
Accession Version	NM_001336527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	maternal effect embryo arrest 22
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). COMPLETENESS: incomplete on the 3' end.

ATGGCGACTG ACGGAGATAC TAAGGAAAAT GGTTCAACTG TGAAAGCATC TTTGGAGAAA 
GAAATATCTC GTTTGAAGTT TGAGATAGTG TCTTTGCAAC AAAAACTGGA GCGGAACCTT 
AAAGAAAAAA GTGAAGAAAC AAAGCTTCTT CAGGATCAAG CTTCTGGTCG AGAAAAGGAG 
ATCAATGAAC TGAGGGATCT CCTTAAGAAA GAAACATTAA GGGCAGATAG TTCTGAAGAA 
GAGAGGGAAC ATGCTTTTAA GGAACTGAAC AAGGCAAAGG CTCTGATAGT AAAAGATGAG 
GAAATCGAGC AAGATATTCC TGAAGTAAAA AGGGAAATAA GTCTGGTTAA AAATCTTCTT 
GCTAGTGAGA GACAGAAGAC AGAGTCAGAG AGGAAGAAAG CAGAATCAGA GAAAAAGAAA 
GCTGATAAGT ATCTTTCGGA GTTGGAGGTG TTAAGAAATT CAGCTCATAA GACTAGTTCT 
GATTTACTTA CTTTGACCTC CAATCTTGAG ACTGTAAAAA AGCAGCTTGA GCTTGAAAAA 
CAGAAGACAC TGAAAGAGAA AAAACGTGCA GATATGGAGA GTGCAAAAGC TAGGGACCAG 
ATGAAGCTGG CAGAAGATGT GTCTAAGAAG TTTGAAATCG TTAGAGCAAG AAATGAAGAG 
CTCAAGAAAG AAATGGAATC GCAGACCGCT AGTAGCCAAG TGAAGTTTGC TGAAAACTCG 
GAAAAGCTGG AAGAGAAGAT AAGGCTCCTT GAGATGAATA AAAAAACAGC CATGGACTGG 
AAATCTCGTA CTGATGATCT GACTCAACAG TTACAAGAAG CGCAATTAGT CGCTGAAGGT 
TTGAAGAAAC AAGTGCATGA GCTTTCACTT TCCCAGAAAT CCATCAAGAC TCACTCTATT 
TCTCCTCAGA AAGTCAGAGA CCTGGAAAAG GCTGAAATGA GGCTTTTGAA AAAAAAGATG 
AAGTTTGAGA GAAATTGTGC AAAACACTCC CAAACAGTGG CTAAATTTGA AAAGTTTCGA 
AGGGAATTTC AGTGCGAAGA GCTAGGTCGG TTGAAACTGG AGTTCGGTAG TCTTACGAAT 
CGCATGAATC TACTGGATGA GTATTTTTCA ACAGACGTTG AAGGTACAGC TGGCCTTGGA 
AAGGCTACAG GATGCAGGAA ACTGCTGACG CTAAATTCAC AGAAGAACCG TAATGGTGAA 
AAGCATTCTG ACGCAAGATG CAAATTGGTA GCCAGCTCAG GTTATCAGGA GCAGGCTTGC 
AAGTTGTCTG CCCACTTAAT TTCTAAATCT GGACGGGGCG TGAGTGAGTC TGTCTCAGGT 
ACTATTTCTC AATTGGAGTC TCCTACTGGA GGCTCTAGAA AATTACCGAG TTCTGGAGTA 
ATTTCCAGCG CAACATCTTT TTCTGATGGG CAGTTACTGG CATCACAGGG AAGAGAGCAG 
TTCTCTGTTA CTACTTCAGC AGAAATAGCA AAAGATAAAC CGAATATCCA ACCAACAAAA 
TCAAGTATGC TCCAAAAGAT CTCCGATACC AGTAAAAATG GAAATCTCTG TTTGGTGGCA 
GAAAACTATC TTCAAAGGTG TCAGAGAGAT ATTCATGAGA ACTCTCGGAA AAGAAAAAGA 
ATGTTAGAGG CAGTTGTGTC CCATAAGCAT CTGGCATCTG GTGATAAAAA GAAAAATTTG 
CCGATTGGAG AGAAGATGGG CACCTTGCAG TCCATGATTG TAGGAACTGG GTCTAGGCCT 
TCAGAAAAGG AAGAAACCTT GGTCCCCCCT GATAGGCAAG GTGGCTCCTC CGCAATTGAC 
ATTACAGTTT CCAAGAAAAG AAGGGTTTCT TGTAAGAAGA AGATAATTGT ACAGAATTCT 
CTTGAGTTCA ACCAGTCAGG CAAGACTCCA GGTAATATAG CTGGGAAAAC TACGTGTCTC 
TCTACTGCTA CTGGACATGA CGTGAAAACA TTATTTTCGG AGGACTTTGC TGCTACAGAT 
TATATGAAGT TGCTAGAGTT GGATAACTTG GAAGAAGAAA ATTATTACCA GATGGCTAGA 
GAATCGCTTC TGTCCCCTGA TCTTCCTCAG GTAGATTTTT TGGGTTGTGA GATCATGAAT 
GAGGACAAAA ATCCTGCTAG GGCTATTGAC TTGGCTGCCA GTAATAGCAT GTATTTACGT 
GAGACCATAC TTTCTAGTGA ATCTCCTAGT CTCAACACTC AGAATATCTC AGTGACAGTT 
GAAATGCCAC CCATGTTGAA ACCGTTGCAT GGGCATCTTC TTAAACACTT TATTGTGTTT 
TCAAATATTG AAGACCAGAA CAGTATCATC ATAATAATTC ATGCTACTAA CAATTGTCTA 
CAGCGTTGCC CGTCAGTTAC TAAAGAACAG TGGGCAGTGC CAGCGATTTT GTCTTCTTTG 
AAAATGGAAG AAAACCTTTT GGCCCAGGAA AGGGCCTGTG TGTTCCTCTC CTTGTTGCTG 
CATAACTTCT CCATGGTTCA CACAACAAAA ACTGGGAATA CTCTGAATGT TGATTCTTTC 
TCCTGCTTGG ATTCTTTCTC AAAGCATATA CGTGGTGTTA TGGCTGATAC TGAAGCTGGA 
GTTATGCTTT CTGGATTTTC GGAAGAACTC CTTTGTCTTC TTCAGGACCT CCTTTCTGGG 
CAGCGGGTAT TATTTTCGGT TAAATCCTCA GAAACATGTG AATCTGATTT AAGCATCCCT 
GTCACCCTGA ATGGAGAAAA TGTAGCTCTC GTCAACAAAA TCGCTCTAAC TGATCAATTG 
GTGGCCGGAA GCGCTATTTT GGCGGCAATA TGTACTGCAC TTGATCGTAT TGGATATATC 
TGCGAAGCTT CCTTTGAAAT CCTGCACAAG TACAGTCATG AGAAAACCTC AGTGCTACTG 
ACCATTCTTC ACGTTTTTGC TTACATTGCT GGAGAGAAAA TGGTGTTGTC TAGTGAGCAT 
GGCATATCAA TTGCAGTGTT GAAATACATT GTCATGTTTC TAGAAAACAA ACATTTTGGT 
ACTGTGGAGG GCAGTTCTCG ATTGCACCCA GGCAAGAACA AGTGTCCATT CTCAGACAGG 
TCTTCCTCGC TGGAGGCTAT GGCATCTAAG CTCATGGAAA TTCTTCAGGA ATTTACTGAG 
TCTAATACTT TGCATAAAAG CTTGACTGGT TCATTGGGTT CTAGCCACCT AGAGAAGACC 
GAGTTTAGGC CGGCACACAA AGATTTCCAG TGTGTATTGA CCAGGGATCA AAGTATCAAT 
CTCTGTGACA TTCTATCATT GGTGGAGCTT ATTGCTTGTT ATACGGCTTG GGATTGGACT 
AGTGCGAACA TTGTTGCTCC ACTGCTTAAG ATGCTGGGAA TGCCATTGCC AATGAACCTC 
TCTGTTGCAA TCGTCTCCCT TCTTGGGCAA CTTAGCAGTA TTGGAGTGGA TGCTGGTGGC 
TATGAAAACG AAGGAATCTC AAACTTGAGA GTGAAACTGT CAGCATTTCT ACAGTGTGAG 
ACGACACTAA AGGCCGGTTT TGCAGTGCAG ATAGCAACTG TGAGCTCCCT CCTGAAGACG 
CTGCAGCTGA AATTCCCAAT AGACTTTCAA GACAAAACCA CCATGATTCC GGGTAGTGGC 
GACCAAAGCT TATCTGGTTC AGTCAATGTG GTGACCAAGT GGTTGTCGTT GTTGAGCAAG 
GAACAACGAG TTTTCGCATT TGAGTTTCTA CAAACCAATG TTGTTAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001324225.1
CDS	86..3796
Translation	MATDGDTKENGSTVKASLEKEISRLKFEIVSLQQKLERNLKEKSEETKLLQDQASGREKEINELRDLLKKETLRADSSEEEREHAFKELNKAKALIVKDEEIEQDIPEVKREISLVKNLLASERQKTESERKKAESEKKKADKYLSELEVLRNSAHKTSSDLLTLTSNLETVKKQLELEKQKTLKEKKRADMESAKARDQMKLAEDVSKKFEIVRARNEELKKEMESQTASSQVKFAENSEKLEEKIRLLEMNKKTAMDWKSRTDDLTQQLQEAQLVAEGLKKQVHELSLSQKSIKTHSISPQKVRDLEKAEMRLLKKKMKFERNCAKHSQTVAKFEKFRREFQCEELGRLKLEFGSLTNRMNLLDEYFSTDVEGTAGLGKATGCRKLLTLNSQKNRNGEKHSDARCKLVASSGYQEQACKLSAHLISKSGRGVSESVSGTISQLESPTGGSRKLPSSGVISSATSFSDGQLLASQGREQFSVTTSAEIAKDKPNIQPTKSSMLQKISDTSKNGNLCLVAENYLQRCQRDIHENSRKRKRMLEAVVSHKHLASGDKKKNLPIGEKMGTLQSMIVGTGSRPSEKEETLVPPDRQGGSSAIDITVSKKRRVSCKKKIIVQNSLEFNQSGKTPGNIAGKTTCLSTATGHDVKTLFSEDFAATDYMKLLELDNLEEENYYQMARESLLSPDLPQVDFLGCEIMNEDKNPARAIDLAASNSMYLRETILSSESPSLNTQNISVTVEMPPMLKPLHGHLLKHFIVFSNIEDQNSIIIIIHATNNCLQRCPSVTKEQWAVPAILSSLKMEENLLAQERACVFLSLLLHNFSMVHTTKTGNTLNVDSFSCLDSFSKHIRGVMADTEAGVMLSGFSEELLCLLQDLLSGQRVLFSVKSSETCESDLSIPVTLNGENVALVNKIALTDQLVAGSAILAAICTALDRIGYICEASFEILHKYSHEKTSVLLTILHVFAYIAGEKMVLSSEHGISIAVLKYIVMFLENKHFGTVEGSSRLHPGKNKCPFSDRSSSLEAMASKLMEILQEFTESNTLHKSLTGSLGSSHLEKTEFRPAHKDFQCVLTRDQSINLCDILSLVELIACYTAWDWTSANIVAPLLKMLGMPLPMNLSVAIVSLLGQLSSIGVDAGGYENEGISNLRVKLSAFLQCETTLKAGFAVQIATVSSLLKTLQLKFPIDFQDKTTMIPGSGDQSLSGSVNVVTKWLSLLSKEQRVFAFEFLQTNVVR

Target ORF information:

RefSeq Version	NM_001336527.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana maternal effect embryo arrest 22 (MEE22), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001336527.1

ORF Insert Sequence:

ATGGCGACTG ACGGAGATAC TAAGGAAAAT GGTTCAACTG TGAAAGCATC TTTGGAGAAA 
GAAATATCTC GTTTGAAGTT TGAGATAGTG TCTTTGCAAC AAAAACTGGA GCGGAACCTT 
AAAGAAAAAA GTGAAGAAAC AAAGCTTCTT CAGGATCAAG CTTCTGGTCG AGAAAAGGAG 
ATCAATGAAC TGAGGGATCT CCTTAAGAAA GAAACATTAA GGGCAGATAG TTCTGAAGAA 
GAGAGGGAAC ATGCTTTTAA GGAACTGAAC AAGGCAAAGG CTCTGATAGT AAAAGATGAG 
GAAATCGAGC AAGATATTCC TGAAGTAAAA AGGGAAATAA GTCTGGTTAA AAATCTTCTT 
GCTAGTGAGA GACAGAAGAC AGAGTCAGAG AGGAAGAAAG CAGAATCAGA GAAAAAGAAA 
GCTGATAAGT ATCTTTCGGA GTTGGAGGTG TTAAGAAATT CAGCTCATAA GACTAGTTCT 
GATTTACTTA CTTTGACCTC CAATCTTGAG ACTGTAAAAA AGCAGCTTGA GCTTGAAAAA 
CAGAAGACAC TGAAAGAGAA AAAACGTGCA GATATGGAGA GTGCAAAAGC TAGGGACCAG 
ATGAAGCTGG CAGAAGATGT GTCTAAGAAG TTTGAAATCG TTAGAGCAAG AAATGAAGAG 
CTCAAGAAAG AAATGGAATC GCAGACCGCT AGTAGCCAAG TGAAGTTTGC TGAAAACTCG 
GAAAAGCTGG AAGAGAAGAT AAGGCTCCTT GAGATGAATA AAAAAACAGC CATGGACTGG 
AAATCTCGTA CTGATGATCT GACTCAACAG TTACAAGAAG CGCAATTAGT CGCTGAAGGT 
TTGAAGAAAC AAGTGCATGA GCTTTCACTT TCCCAGAAAT CCATCAAGAC TCACTCTATT 
TCTCCTCAGA AAGTCAGAGA CCTGGAAAAG GCTGAAATGA GGCTTTTGAA AAAAAAGATG 
AAGTTTGAGA GAAATTGTGC AAAACACTCC CAAACAGTGG CTAAATTTGA AAAGTTTCGA 
AGGGAATTTC AGTGCGAAGA GCTAGGTCGG TTGAAACTGG AGTTCGGTAG TCTTACGAAT 
CGCATGAATC TACTGGATGA GTATTTTTCA ACAGACGTTG AAGGTACAGC TGGCCTTGGA 
AAGGCTACAG GATGCAGGAA ACTGCTGACG CTAAATTCAC AGAAGAACCG TAATGGTGAA 
AAGCATTCTG ACGCAAGATG CAAATTGGTA GCCAGCTCAG GTTATCAGGA GCAGGCTTGC 
AAGTTGTCTG CCCACTTAAT TTCTAAATCT GGACGGGGCG TGAGTGAGTC TGTCTCAGGT 
ACTATTTCTC AATTGGAGTC TCCTACTGGA GGCTCTAGAA AATTACCGAG TTCTGGAGTA 
ATTTCCAGCG CAACATCTTT TTCTGATGGG CAGTTACTGG CATCACAGGG AAGAGAGCAG 
TTCTCTGTTA CTACTTCAGC AGAAATAGCA AAAGATAAAC CGAATATCCA ACCAACAAAA 
TCAAGTATGC TCCAAAAGAT CTCCGATACC AGTAAAAATG GAAATCTCTG TTTGGTGGCA 
GAAAACTATC TTCAAAGGTG TCAGAGAGAT ATTCATGAGA ACTCTCGGAA AAGAAAAAGA 
ATGTTAGAGG CAGTTGTGTC CCATAAGCAT CTGGCATCTG GTGATAAAAA GAAAAATTTG 
CCGATTGGAG AGAAGATGGG CACCTTGCAG TCCATGATTG TAGGAACTGG GTCTAGGCCT 
TCAGAAAAGG AAGAAACCTT GGTCCCCCCT GATAGGCAAG GTGGCTCCTC CGCAATTGAC 
ATTACAGTTT CCAAGAAAAG AAGGGTTTCT TGTAAGAAGA AGATAATTGT ACAGAATTCT 
CTTGAGTTCA ACCAGTCAGG CAAGACTCCA GGTAATATAG CTGGGAAAAC TACGTGTCTC 
TCTACTGCTA CTGGACATGA CGTGAAAACA TTATTTTCGG AGGACTTTGC TGCTACAGAT 
TATATGAAGT TGCTAGAGTT GGATAACTTG GAAGAAGAAA ATTATTACCA GATGGCTAGA 
GAATCGCTTC TGTCCCCTGA TCTTCCTCAG GTAGATTTTT TGGGTTGTGA GATCATGAAT 
GAGGACAAAA ATCCTGCTAG GGCTATTGAC TTGGCTGCCA GTAATAGCAT GTATTTACGT 
GAGACCATAC TTTCTAGTGA ATCTCCTAGT CTCAACACTC AGAATATCTC AGTGACAGTT 
GAAATGCCAC CCATGTTGAA ACCGTTGCAT GGGCATCTTC TTAAACACTT TATTGTGTTT 
TCAAATATTG AAGACCAGAA CAGTATCATC ATAATAATTC ATGCTACTAA CAATTGTCTA 
CAGCGTTGCC CGTCAGTTAC TAAAGAACAG TGGGCAGTGC CAGCGATTTT GTCTTCTTTG 
AAAATGGAAG AAAACCTTTT GGCCCAGGAA AGGGCCTGTG TGTTCCTCTC CTTGTTGCTG 
CATAACTTCT CCATGGTTCA CACAACAAAA ACTGGGAATA CTCTGAATGT TGATTCTTTC 
TCCTGCTTGG ATTCTTTCTC AAAGCATATA CGTGGTGTTA TGGCTGATAC TGAAGCTGGA 
GTTATGCTTT CTGGATTTTC GGAAGAACTC CTTTGTCTTC TTCAGGACCT CCTTTCTGGG 
CAGCGGGTAT TATTTTCGGT TAAATCCTCA GAAACATGTG AATCTGATTT AAGCATCCCT 
GTCACCCTGA ATGGAGAAAA TGTAGCTCTC GTCAACAAAA TCGCTCTAAC TGATCAATTG 
GTGGCCGGAA GCGCTATTTT GGCGGCAATA TGTACTGCAC TTGATCGTAT TGGATATATC 
TGCGAAGCTT CCTTTGAAAT CCTGCACAAG TACAGTCATG AGAAAACCTC AGTGCTACTG 
ACCATTCTTC ACGTTTTTGC TTACATTGCT GGAGAGAAAA TGGTGTTGTC TAGTGAGCAT 
GGCATATCAA TTGCAGTGTT GAAATACATT GTCATGTTTC TAGAAAACAA ACATTTTGGT 
ACTGTGGAGG GCAGTTCTCG ATTGCACCCA GGCAAGAACA AGTGTCCATT CTCAGACAGG 
TCTTCCTCGC TGGAGGCTAT GGCATCTAAG CTCATGGAAA TTCTTCAGGA ATTTACTGAG 
TCTAATACTT TGCATAAAAG CTTGACTGGT TCATTGGGTT CTAGCCACCT AGAGAAGACC 
GAGTTTAGGC CGGCACACAA AGATTTCCAG TGTGTATTGA CCAGGGATCA AAGTATCAAT 
CTCTGTGACA TTCTATCATT GGTGGAGCTT ATTGCTTGTT ATACGGCTTG GGATTGGACT 
AGTGCGAACA TTGTTGCTCC ACTGCTTAAG ATGCTGGGAA TGCCATTGCC AATGAACCTC 
TCTGTTGCAA TCGTCTCCCT TCTTGGGCAA CTTAGCAGTA TTGGAGTGGA TGCTGGTGGC 
TATGAAAACG AAGGAATCTC AAACTTGAGA GTGAAACTGT CAGCATTTCT ACAGTGTGAG 
ACGACACTAA AGGCCGGTTT TGCAGTGCAG ATAGCAACTG TGAGCTCCCT CCTGAAGACG 
CTGCAGCTGA AATTCCCAAT AGACTTTCAA GACAAAACCA CCATGATTCC GGGTAGTGGC 
GACCAAAGCT TATCTGGTTC AGTCAATGTG GTGACCAAGT GGTTGTCGTT GTTGAGCAAG 
GAACAACGAG TTTTCGCATT TGAGTTTCTA CAAACCAATG TTGTTAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Nature402(6763)761-8(1999 Dec)
Lin X,Kaul S,Rounsley S,Shea TP,Benito MI,Town CD,Fujii CY,Mason T,Bowman CL,Barnstead M,Feldblyum TV,Buell CR,Ketchum KA,Lee J,Ronning CM,Koo HL,Moffat KS,Cronin LA,Shen M,Pai G,Van Aken S,Umayam L,Tallon LJ,Gill JE,Adams MD,Carrera AJ,Creasy TH,Goodman HM,Somerville CR,Copenhaver GP,Preuss D,Nierman WC,White O,Eisen JA,Salzberg SL,Fraser CM,Venter JC

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MEE22 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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