AT2G20050 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT2G20050
Entrez Gene ID	816524
Full Name	protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein
Synonyms	T2G17.15,T2G17_15
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_127563.6	NP_179595.5	protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein
NM_001202628.1	NP_001189557.1	protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT2G20050	NP_179595.5
Danio rerio (zebrafish)	si:dkey-121j17.5	XP_002665675.3
Oryza sativa (rice)	Os02g0281000	NP_001046559.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Lin X, Kaul S, Rounsley S, Shea TP, Benito MI, Town CD, Fujii CY, Mason T, Bowman CL, Barnstead M, Feldblyum TV, Buell CR, Ketchum KA, Lee J, Ronning CM, Koo HL, Moffat KS, Cronin LA, Shen M, Pai G, Van Aken S, Umayam L, Tallon LJ, Gill JE, Adams MD, Carrera AJ, Creasy TH, Goodman HM, Somerville CR, Copenhaver GP, Preuss D, Nierman WC, White O, Eisen JA, Salzberg SL, Fraser CM, Venter JC
Nature402(6763)761-8(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AT2G20050 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT2G20050 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb07443	NM_127563.6 Latest version!	Arabidopsis thaliana protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein (AT2G20050), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OAb30757	NM_001202628.1 Latest version!	Arabidopsis thaliana protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein (AT2G20050), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT2G20050 ( NM_127563.6 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_179595.5 Arabidopsis thaliana protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein (AT2G20050), mRNA.

Price & Availability↑

CloneID	OAb07443
Clone ID Related Accession (Same CDS sequence)	NM_127563.6
Accession Version	NM_127563.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). On Sep 12, 2016 this sequence version replaced NM_127563.5.

ATGGGTTGTG CTTATTCCAA AACTTGCATT GGTCAGATTT GTGCTACGAA AGAGAATAGT 
ATCAGGCAAA CGCATCAACA AGCTCCGTCC AGAGGTGGCA CGAGAGCTAC TGCGGCGGCG 
GCGGCGGTTG AGGAGGATAA TCCGGTGTTT AATTTCTCTT CAGATGCGGT GGATGATGTA 
GATAACGACG AAATTCATCA GCTTGGTTTG AGTAGAGACC AGGAATGGGG GATTACTCGG 
CTTTCTAGGG TTTCTTCTCA ATTTCTACCA CCAGATGGGT CAAGGGTTGT CAAGGTTCCG 
TCTTGTAACT ATGAATTGAG ATGCTCGTTT TTGTCTCAAA GAGGGTATTA TCCTGATGCT 
CTTGATAAGG CCAATCAAGA TAGTTTCGCT ATTCATACTC CTTTTGGGAG TAATTCGGAT 
GATCATTTCT TTGGGGTTTT TGATGGGCAT GGTGAGTTTG GTGCTCAGTG TTCTCAGTTT 
GTGAAGCGGA GGTTGTGTGA GAATCTTCTT AGACATGGTA GGTTTCGTGT GGATCCTGCT 
GAGGCTTGTA ATTCGGCTTT CTTGACCACG AATTCTCAGT TACATGCTGA TTTGGTAGAT 
GATAGTATGA GTGGGACTAC TGCGATTACG GTTATGGTTA GAGGAAGGAC GATATATGTG 
GCGAATGCAG GTGATTCCAG GGCGGTTTTA GCTGAGAAGA GGGATGGTGA TCTTGTAGCT 
GTTGATTTGT CTATTGACCA GACTCCTTTT AGACCTGATG AGCTTGAAAG GGTTAAGCTT 
TGTGGTGCTA GAGTTCTTAC TCTTGATCAG ATTGAAGGCT TGAAAAATCC TGATGTTCAG 
TGTTGGGGTA CTGAGGAAGA TGATGATGGA GACCCTCCGA GACTTTGGGT GCCTAATGGA 
ATGTATCCTG GAACTGCTTT TACCAGGAGT ATTGGTGATT CTATTGCAGA GACAATTGGT 
GTTGTTGCTA ACCCTGAGAT TGCTGTTGTT GAGCTCACAC CGGATAATCC TTTCTTTGTG 
GTTGCTAGTG ATGGCGTATT TGAGTTCATC TCTAGTCAAA CGGTGGTTGA CATGGTTGCA 
AAACATAAGG ATCCTCGAGA TGCTTGTGCT GCAATTGTTG CTGAATCATA TAGGCTATGG 
CTACAGTATG AAACTCGGAC AGATGATATA ACAATCATCG TCGTGCACAT TGATGGACTA 
AAAGATGATG CTCCTCGGCA ATTGTCGAGT ACAGGAACTC AATTGCAGCC TCCTATTCCA 
CAAGTTGTGG AGCTTACAGG TTCGGAATCT CCTTCCACCT TTGGATGGAA CTCTAAAAAT 
CAACGTGTGA GGCATGATCT ATCTCGAGCT AGGATACGTG CCATTGAGAA TTCACTAGAG 
AATGGACATG CTTGGGTTCC TCCATCTCCA GCCCACAGAA AAACCTGGGA AGAAGAAGCA 
CACATTGAGC GGGTGTTGCG TGACCATTTC CTCTTCAGGA AACTCACTGA TTCTCAGTGC 
CAGGTTTTGT TGGACTGCAT GCAAAGGCTT GAAGCTAATC CAGGGGATAT TGTTGTGAAA 
CAGGGTGGTG AAGGGGACTG CTTCTACGTT GTGGGTAGTG GAGAATTCGA GGTCTTAGCA 
ACTCAGGATG GAAAGAACGG TGAGGTACCT AGGATCTTGC AGCGTTATAC AGCTGAGAAA 
CAATCATCAT TCGGTGAACT CGCCTTAATG CATAACAAGC CGCTTCAGGC TTCTGTACGT 
GCTGTAGATC ACGGAACATT GTGGGCCTTA AAAAGAGAAG ATTTTCGAGG AATTTTGATG 
TCTGAGTTTT CAAACTTAGC ATCCTTGAAG CTTCTTCGTT CTGTCGATCT TCTTTCCCGT 
CTTACAATTT TGCAACTAAG CCATGTTGCA GAGTCTCTTT CCGAAGCTTG CTTTTCTGAT 
GGACAAACAA TTGTTACCAA GGACCAAAAA CTTCAGGGCC TGTATGTTAT CCAGAAGGGA 
CGCGTAAAAA TATCTTTCTG TACAGAGGTG TTGGAGAGTC AAAATGTTTC AAGCCTTACA 
ACTGGAATCA CTAACGAGTA TGACAATCTT GAAATCGGAA CAGAAGTCTC CATAGAAAAG 
CATGAAGGAA GTTACTTTGG TGAATGGGCT CTTCTTGGCG AACTCAAAGA TTCCTTAAGT 
GTGGTTGCAG TTGGCGAAGT GGTTTGTGTG GTTTTAACAA AAGAAAATTT CGAGTCAGCG 
GTCGGCCCTC TGACCAATAT TTCAGATGAC GGTCCCAAGA CAAGACATTC TTCATTCGAG 
CTGTCAAAGG AATCTGCAAA AGTTACCGAT ACCACAGCTC TTGCTAAAGC CACTCTTGCA 
GACCTGGAAT GGACAACATG CTTGAGTACA ACAGACTGTA GTGAGATTGG GCTCGTGCAT 
TTGAAAGATA AAGAAAATTT GCTCAGCTTG AAAAGATTTT CAAAGCAAAA GGTGAAAAAG 
TTAGGTAAAG AGGCACAAGT ATTGAAAGAG CGGAATCTGA TGAAGAACGT AATAAAGCCC 
TCAGCTATTG TTCCCGAAAT CTTGTGTACT TGTGTCGATC AAACATTTGC GGCAATCTTA 
CTGAACACTA CTCTTGCCTG TCCTATCTCT TCTCTGCTTC ACTCTCCGCT TGACGAGTCA 
TCTGTCCGTT TCATTACCGG CTCACTTGTG TCTGCCATAG AAGACATACA CAAGAACGAG 
ATTCTCTTCA GAGGTTCATC CCCCGAGTTA CTGATGTTGG ATCAATCCGG ATATCTACAG 
ATTGTAGACT TCAGATTCGC CAAGAAATTG TCCGGGGAAC GAACATTTAC AATCTGTGGA 
AATGCAGATT ATTTAGCCCC AGAAATTGTT CAAGGCAAAG GCCATGGTTA TGCAGCTGAC 
TGGTGGGCTC TTGGAGTTTT GATCTACTAT ATGCTAGAAG GAGAAATGCC ATTCGGGTCA 
TGGAGAGAGA GCGAGCTCGA CACGTTTCAG AAGATTGCAA AAGGCCAACT AACTTTCCCT 
CGAGTTCTAA GCTCAGAAGC TGAAGATCTC ATCACAAAGT TGCTTGAAGT TGATGAAAAC 
CTGCGGTTTG GTAGCCAAGG AGGTCCAGAA TCAATCAAGA AACATCCCTG GTTCAATGGT 
CTTAAATGGG AAGCTATTAG TAACCGCGAA TTTCAAGTTC CTCAAGAGAT AATCTCGCGA 
ATTCATCACC ATTTGGAGAA TGATAACGTT CTCCCTCTAG AAACTTCTAA ATCGCTTGAC 
ACAACAGAAG ATCAAGATGC TCAAAACTGG CTTGAAGAAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_179595.5
CDS	627..3911
Translation	MGCAYSKTCIGQICATKENSIRQTHQQAPSRGGTRATAAAAAVEEDNPVFNFSSDAVDDVDNDEIHQLGLSRDQEWGITRLSRVSSQFLPPDGSRVVKVPSCNYELRCSFLSQRGYYPDALDKANQDSFAIHTPFGSNSDDHFFGVFDGHGEFGAQCSQFVKRRLCENLLRHGRFRVDPAEACNSAFLTTNSQLHADLVDDSMSGTTAITVMVRGRTIYVANAGDSRAVLAEKRDGDLVAVDLSIDQTPFRPDELERVKLCGARVLTLDQIEGLKNPDVQCWGTEEDDDGDPPRLWVPNGMYPGTAFTRSIGDSIAETIGVVANPEIAVVELTPDNPFFVVASDGVFEFISSQTVVDMVAKHKDPRDACAAIVAESYRLWLQYETRTDDITIIVVHIDGLKDDAPRQLSSTGTQLQPPIPQVVELTGSESPSTFGWNSKNQRVRHDLSRARIRAIENSLENGHAWVPPSPAHRKTWEEEAHIERVLRDHFLFRKLTDSQCQVLLDCMQRLEANPGDIVVKQGGEGDCFYVVGSGEFEVLATQDGKNGEVPRILQRYTAEKQSSFGELALMHNKPLQASVRAVDHGTLWALKREDFRGILMSEFSNLASLKLLRSVDLLSRLTILQLSHVAESLSEACFSDGQTIVTKDQKLQGLYVIQKGRVKISFCTEVLESQNVSSLTTGITNEYDNLEIGTEVSIEKHEGSYFGEWALLGELKDSLSVVAVGEVVCVVLTKENFESAVGPLTNISDDGPKTRHSSFELSKESAKVTDTTALAKATLADLEWTTCLSTTDCSEIGLVHLKDKENLLSLKRFSKQKVKKLGKEAQVLKERNLMKNVIKPSAIVPEILCTCVDQTFAAILLNTTLACPISSLLHSPLDESSVRFITGSLVSAIEDIHKNEILFRGSSPELLMLDQSGYLQIVDFRFAKKLSGERTFTICGNADYLAPEIVQGKGHGYAADWWALGVLIYYMLEGEMPFGSWRESELDTFQKIAKGQLTFPRVLSSEAEDLITKLLEVDENLRFGSQGGPESIKKHPWFNGLKWEAISNREFQVPQEIISRIHHHLENDNVLPLETSKSLDTTEDQDAQNWLEEW

Target ORF information:

RefSeq Version	NM_127563.6
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein (AT2G20050), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_127563.6

ORF Insert Sequence:

ATGGGTTGTG CTTATTCCAA AACTTGCATT GGTCAGATTT GTGCTACGAA AGAGAATAGT 
ATCAGGCAAA CGCATCAACA AGCTCCGTCC AGAGGTGGCA CGAGAGCTAC TGCGGCGGCG 
GCGGCGGTTG AGGAGGATAA TCCGGTGTTT AATTTCTCTT CAGATGCGGT GGATGATGTA 
GATAACGACG AAATTCATCA GCTTGGTTTG AGTAGAGACC AGGAATGGGG GATTACTCGG 
CTTTCTAGGG TTTCTTCTCA ATTTCTACCA CCAGATGGGT CAAGGGTTGT CAAGGTTCCG 
TCTTGTAACT ATGAATTGAG ATGCTCGTTT TTGTCTCAAA GAGGGTATTA TCCTGATGCT 
CTTGATAAGG CCAATCAAGA TAGTTTCGCT ATTCATACTC CTTTTGGGAG TAATTCGGAT 
GATCATTTCT TTGGGGTTTT TGATGGGCAT GGTGAGTTTG GTGCTCAGTG TTCTCAGTTT 
GTGAAGCGGA GGTTGTGTGA GAATCTTCTT AGACATGGTA GGTTTCGTGT GGATCCTGCT 
GAGGCTTGTA ATTCGGCTTT CTTGACCACG AATTCTCAGT TACATGCTGA TTTGGTAGAT 
GATAGTATGA GTGGGACTAC TGCGATTACG GTTATGGTTA GAGGAAGGAC GATATATGTG 
GCGAATGCAG GTGATTCCAG GGCGGTTTTA GCTGAGAAGA GGGATGGTGA TCTTGTAGCT 
GTTGATTTGT CTATTGACCA GACTCCTTTT AGACCTGATG AGCTTGAAAG GGTTAAGCTT 
TGTGGTGCTA GAGTTCTTAC TCTTGATCAG ATTGAAGGCT TGAAAAATCC TGATGTTCAG 
TGTTGGGGTA CTGAGGAAGA TGATGATGGA GACCCTCCGA GACTTTGGGT GCCTAATGGA 
ATGTATCCTG GAACTGCTTT TACCAGGAGT ATTGGTGATT CTATTGCAGA GACAATTGGT 
GTTGTTGCTA ACCCTGAGAT TGCTGTTGTT GAGCTCACAC CGGATAATCC TTTCTTTGTG 
GTTGCTAGTG ATGGCGTATT TGAGTTCATC TCTAGTCAAA CGGTGGTTGA CATGGTTGCA 
AAACATAAGG ATCCTCGAGA TGCTTGTGCT GCAATTGTTG CTGAATCATA TAGGCTATGG 
CTACAGTATG AAACTCGGAC AGATGATATA ACAATCATCG TCGTGCACAT TGATGGACTA 
AAAGATGATG CTCCTCGGCA ATTGTCGAGT ACAGGAACTC AATTGCAGCC TCCTATTCCA 
CAAGTTGTGG AGCTTACAGG TTCGGAATCT CCTTCCACCT TTGGATGGAA CTCTAAAAAT 
CAACGTGTGA GGCATGATCT ATCTCGAGCT AGGATACGTG CCATTGAGAA TTCACTAGAG 
AATGGACATG CTTGGGTTCC TCCATCTCCA GCCCACAGAA AAACCTGGGA AGAAGAAGCA 
CACATTGAGC GGGTGTTGCG TGACCATTTC CTCTTCAGGA AACTCACTGA TTCTCAGTGC 
CAGGTTTTGT TGGACTGCAT GCAAAGGCTT GAAGCTAATC CAGGGGATAT TGTTGTGAAA 
CAGGGTGGTG AAGGGGACTG CTTCTACGTT GTGGGTAGTG GAGAATTCGA GGTCTTAGCA 
ACTCAGGATG GAAAGAACGG TGAGGTACCT AGGATCTTGC AGCGTTATAC AGCTGAGAAA 
CAATCATCAT TCGGTGAACT CGCCTTAATG CATAACAAGC CGCTTCAGGC TTCTGTACGT 
GCTGTAGATC ACGGAACATT GTGGGCCTTA AAAAGAGAAG ATTTTCGAGG AATTTTGATG 
TCTGAGTTTT CAAACTTAGC ATCCTTGAAG CTTCTTCGTT CTGTCGATCT TCTTTCCCGT 
CTTACAATTT TGCAACTAAG CCATGTTGCA GAGTCTCTTT CCGAAGCTTG CTTTTCTGAT 
GGACAAACAA TTGTTACCAA GGACCAAAAA CTTCAGGGCC TGTATGTTAT CCAGAAGGGA 
CGCGTAAAAA TATCTTTCTG TACAGAGGTG TTGGAGAGTC AAAATGTTTC AAGCCTTACA 
ACTGGAATCA CTAACGAGTA TGACAATCTT GAAATCGGAA CAGAAGTCTC CATAGAAAAG 
CATGAAGGAA GTTACTTTGG TGAATGGGCT CTTCTTGGCG AACTCAAAGA TTCCTTAAGT 
GTGGTTGCAG TTGGCGAAGT GGTTTGTGTG GTTTTAACAA AAGAAAATTT CGAGTCAGCG 
GTCGGCCCTC TGACCAATAT TTCAGATGAC GGTCCCAAGA CAAGACATTC TTCATTCGAG 
CTGTCAAAGG AATCTGCAAA AGTTACCGAT ACCACAGCTC TTGCTAAAGC CACTCTTGCA 
GACCTGGAAT GGACAACATG CTTGAGTACA ACAGACTGTA GTGAGATTGG GCTCGTGCAT 
TTGAAAGATA AAGAAAATTT GCTCAGCTTG AAAAGATTTT CAAAGCAAAA GGTGAAAAAG 
TTAGGTAAAG AGGCACAAGT ATTGAAAGAG CGGAATCTGA TGAAGAACGT AATAAAGCCC 
TCAGCTATTG TTCCCGAAAT CTTGTGTACT TGTGTCGATC AAACATTTGC GGCAATCTTA 
CTGAACACTA CTCTTGCCTG TCCTATCTCT TCTCTGCTTC ACTCTCCGCT TGACGAGTCA 
TCTGTCCGTT TCATTACCGG CTCACTTGTG TCTGCCATAG AAGACATACA CAAGAACGAG 
ATTCTCTTCA GAGGTTCATC CCCCGAGTTA CTGATGTTGG ATCAATCCGG ATATCTACAG 
ATTGTAGACT TCAGATTCGC CAAGAAATTG TCCGGGGAAC GAACATTTAC AATCTGTGGA 
AATGCAGATT ATTTAGCCCC AGAAATTGTT CAAGGCAAAG GCCATGGTTA TGCAGCTGAC 
TGGTGGGCTC TTGGAGTTTT GATCTACTAT ATGCTAGAAG GAGAAATGCC ATTCGGGTCA 
TGGAGAGAGA GCGAGCTCGA CACGTTTCAG AAGATTGCAA AAGGCCAACT AACTTTCCCT 
CGAGTTCTAA GCTCAGAAGC TGAAGATCTC ATCACAAAGT TGCTTGAAGT TGATGAAAAC 
CTGCGGTTTG GTAGCCAAGG AGGTCCAGAA TCAATCAAGA AACATCCCTG GTTCAATGGT 
CTTAAATGGG AAGCTATTAG TAACCGCGAA TTTCAAGTTC CTCAAGAGAT AATCTCGCGA 
ATTCATCACC ATTTGGAGAA TGATAACGTT CTCCCTCTAG AAACTTCTAA ATCGCTTGAC 
ACAACAGAAG ATCAAGATGC TCAAAACTGG CTTGAAGAAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT2G20050 ( NM_001202628.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001189557.1 Arabidopsis thaliana protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein (AT2G20050), mRNA.

Price & Availability↑

CloneID	OAb30757
Clone ID Related Accession (Same CDS sequence)	NM_001202628.1
Accession Version	NM_001202628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071).

ATGGGTTGTG CTTATTCCAA AACTTGCATT GGTCAGATTT GTGCTACGAA AGAGAATAGT 
ATCAGGCAAA CGCATCAACA AGCTCCGTCC AGAGGTGGCA CGAGAGCTAC TGCGGCGGCG 
GCGGCGGTTG AGGAGGATAA TCCGGTGTTT AATTTCTCTT CAGATGCGGT GGATGATGTA 
GATAACGACG AAATTCATCA GCTTGGTTTG AGTAGAGACC AGGAATGGGG GATTACTCGG 
CTTTCTAGGG TTTCTTCTCA ATTTCTACCA CCAGATGGGT CAAGGGTTGT CAAGGTTCCG 
TCTTGTAACT ATGAATTGAG ATGCTCGTTT TTGTCTCAAA GAGGGTATTA TCCTGATGCT 
CTTGATAAGG CCAATCAAGA TAGTTTCGCT ATTCATACTC CTTTTGGGAG TAATTCGGAT 
GATCATTTCT TTGGGGTTTT TGATGGGCAT GGTGAGTTTG GTGCTCAGTG TTCTCAGTTT 
GTGAAGCGGA GGTTGTGTGA GAATCTTCTT AGACATGGTA GGTTTCGTGT GGATCCTGCT 
GAGGCTTGTA ATTCGGCTTT CTTGACCACG AATTCTCAGT TACATGCTGA TTTGGTAGAT 
GATAGTATGA GTGGGACTAC TGCGATTACG GTTATGGTTA GAGGAAGGAC GATATATGTG 
GCGAATGCAG GTGATTCCAG GGCGGTTTTA GCTGAGAAGA GGGATGGTGA TCTTGTAGCT 
GTTGATTTGT CTATTGACCA GACTCCTTTT AGACCTGATG AGCTTGAAAG GGTTAAGCTT 
TGTGGTGCTA GAGTTCTTAC TCTTGATCAG ATTGAAGGCT TGAAAAATCC TGATGTTCAG 
TGTTGGGGTA CTGAGGAAGA TGATGATGGA GACCCTCCGA GACTTTGGGT GCCTAATGGA 
ATGTATCCTG GAACTGCTTT TACCAGGAGT ATTGGTGATT CTATTGCAGA GACAATTGGT 
GTTGTTGCTA ACCCTGAGAT TGCTGTTGTT GAGCTCACAC CGGATAATCC TTTCTTTGTG 
GTTGCTAGTG ATGGCGTATT TGAGTTCATC TCTAGTCAAA CGGTGGTTGA CATGGTTGCA 
AAACATAAGG ATCCTCGAGA TGCTTGTGCT GCAATTGTTG CTGAATCATA TAGGCTATGG 
CTACAGTATG AAACTCGGAC AGATGATATA ACAATCATCG TCGTGCACAT TGATGGACTA 
AAAGATGATG CTCCTCGGCA ATTGTCGAGT ACAGGAACTC AATTGCAGCC TCCTATTCCA 
CAAGTTGTGG AGCTTACAGG TTCGGAATCT CCTTCCACCT TTGGATGGAA CTCTAAAAAT 
CAACGTGTGA GGCATGATCT ATCTCGAGCT AGGATACGTG CCATTGAGAA TTCACTAGAG 
AATGGACATG CTTGGGTTCC TCCATCTCCA GCCCACAGAA AAACCTGGGA AGAAGAAGCA 
CACATTGAGC GGGTGTTGCG TGACCATTTC CTCTTCAGGA AACTCACTGA TTCTCAGTGC 
CAGGTTTTGT TGGACTGCAT GCAAAGGCTT GAAGCTAATC CAGGGGATAT TGTTGTGAAA 
CAGGGTGGTG AAGGGGACTG CTTCTACGTT GTGGGTAGTG GAGAATTCGA GGTCTTAGCA 
ACTCAGAACG GTGAGGTACC TAGGATCTTG CAGCGTTATA CAGCTGAGAA ACAATCATCA 
TTCGGTGAAC TCGCCTTAAT GCATAACAAG CCGCTTCAGG CTTCTGTACG TGCTGTAGAT 
CACGGAACAT TGTGGGCCTT AAAAAGAGAA GATTTTCGAG GAATTTTGAT GTCTGAGTTT 
TCAAACTTAG CATCCTTGAA GCTTCTTCGT TCTGTCGATC TTCTTTCCCG TCTTACAATT 
TTGCAACTAA GCCATGTTGC AGAGTCTCTT TCCGAAGCTT GCTTTTCTGA TGGACAAACA 
ATTGTTACCA AGGACCAAAA ACTTCAGGGC CTGTATGTTA TCCAGAAGGG ACGCGTAAAA 
ATATCTTTCT GTACAGAGGT GTTGGAGAGT CAAAATGTTT CAAGCCTTAC AACTGGAATC 
ACTAACGAGT ATGACAATCT TGAAATCGGA ACAGAAGTCT CCATAGAAAA GCATGAAGGA 
AGTTACTTTG GTGAATGGGC TCTTCTTGGC GAACTCAAAG ATTCCTTAAG TGTGGTTGCA 
GTTGGCGAAG TGGTTTGTGT GGTTTTAACA AAAGAAAATT TCGAGTCAGC GGTCGGCCCT 
CTGACCAATA TTTCAGATGA CGGTCCCAAG ACAAGACATT CTTCATTCGA GCTGTCAAAG 
GAATCTGCAA AAGTTACCGA TACCACAGCT CTTGCTAAAG CCACTCTTGC AGACCTGGAA 
TGGACAACAT GCTTGAGTAC AACAGACTGT AGTGAGATTG GGCTCGTGCA TTTGAAAGAT 
AAAGAAAATT TGCTCAGCTT GAAAAGATTT TCAAAGCAAA AGGTGAAAAA GTTAGGTAAA 
GAGGCACAAG TATTGAAAGA GCGGAATCTG ATGAAGAACG TAATAAAGCC CTCAGCTATT 
GTTCCCGAAA TCTTGTGTAC TTGTGTCGAT CAAACATTTG CGGCAATCTT ACTGAACACT 
ACTCTTGCCT GTCCTATCTC TTCTCTGCTT CACTCTCCGC TTGACGAGTC ATCTGTCCGT 
TTCATTACCG GCTCACTTGT GTCTGCCATA GAAGACATAC ACAAGAACGA GATTCTCTTC 
AGAGGTTCAT CCCCCGAGTT ACTGATGTTG GATCAATCCG GATATCTACA GATTGTAGAC 
TTCAGATTCG CCAAGAAATT GTCCGGGGAA CGAACATTTA CAATCTGTGG AAATGCAGAT 
TATTTAGCCC CAGAAATTGT TCAAGGCAAA GGCCATGGTT ATGCAGCTGA CTGGTGGGCT 
CTTGGAGTTT TGATCTACTA TATGCTAGAA GGAGAAATGC CATTCGGGTC ATGGAGAGAG 
AGCGAGCTCG ACACGTTTCA GAAGATTGCA AAAGGCCAAC TAACTTTCCC TCGAGTTCTA 
AGCTCAGAAG CTGAAGATCT CATCACAAAG TTGCTTGAAG TTGATGAAAA CCTGCGGTTT 
GGTAGCCAAG GAGGTCCAGA ATCAATCAAG AAACATCCCT GGTTCAATGG TCTTAAATGG 
GAAGCTATTA GTAACCGCGA ATTTCAAGTT CCTCAAGAGA TAATCTCGCG AATTCATCAC 
CATTTGGAGA ATGATAACGT TCTCCCTCTA GAAACTTCTA AATCGCTTGA CACAACAGAA 
GATCAAGATG CTCAAAACTG GCTTGAAGAA TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189557.1
CDS	258..3533
Translation	MGCAYSKTCIGQICATKENSIRQTHQQAPSRGGTRATAAAAAVEEDNPVFNFSSDAVDDVDNDEIHQLGLSRDQEWGITRLSRVSSQFLPPDGSRVVKVPSCNYELRCSFLSQRGYYPDALDKANQDSFAIHTPFGSNSDDHFFGVFDGHGEFGAQCSQFVKRRLCENLLRHGRFRVDPAEACNSAFLTTNSQLHADLVDDSMSGTTAITVMVRGRTIYVANAGDSRAVLAEKRDGDLVAVDLSIDQTPFRPDELERVKLCGARVLTLDQIEGLKNPDVQCWGTEEDDDGDPPRLWVPNGMYPGTAFTRSIGDSIAETIGVVANPEIAVVELTPDNPFFVVASDGVFEFISSQTVVDMVAKHKDPRDACAAIVAESYRLWLQYETRTDDITIIVVHIDGLKDDAPRQLSSTGTQLQPPIPQVVELTGSESPSTFGWNSKNQRVRHDLSRARIRAIENSLENGHAWVPPSPAHRKTWEEEAHIERVLRDHFLFRKLTDSQCQVLLDCMQRLEANPGDIVVKQGGEGDCFYVVGSGEFEVLATQNGEVPRILQRYTAEKQSSFGELALMHNKPLQASVRAVDHGTLWALKREDFRGILMSEFSNLASLKLLRSVDLLSRLTILQLSHVAESLSEACFSDGQTIVTKDQKLQGLYVIQKGRVKISFCTEVLESQNVSSLTTGITNEYDNLEIGTEVSIEKHEGSYFGEWALLGELKDSLSVVAVGEVVCVVLTKENFESAVGPLTNISDDGPKTRHSSFELSKESAKVTDTTALAKATLADLEWTTCLSTTDCSEIGLVHLKDKENLLSLKRFSKQKVKKLGKEAQVLKERNLMKNVIKPSAIVPEILCTCVDQTFAAILLNTTLACPISSLLHSPLDESSVRFITGSLVSAIEDIHKNEILFRGSSPELLMLDQSGYLQIVDFRFAKKLSGERTFTICGNADYLAPEIVQGKGHGYAADWWALGVLIYYMLEGEMPFGSWRESELDTFQKIAKGQLTFPRVLSSEAEDLITKLLEVDENLRFGSQGGPESIKKHPWFNGLKWEAISNREFQVPQEIISRIHHHLENDNVLPLETSKSLDTTEDQDAQNWLEEW

Target ORF information:

RefSeq Version	NM_001202628.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein (AT2G20050), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202628.1

ORF Insert Sequence:

ATGGGTTGTG CTTATTCCAA AACTTGCATT GGTCAGATTT GTGCTACGAA AGAGAATAGT 
ATCAGGCAAA CGCATCAACA AGCTCCGTCC AGAGGTGGCA CGAGAGCTAC TGCGGCGGCG 
GCGGCGGTTG AGGAGGATAA TCCGGTGTTT AATTTCTCTT CAGATGCGGT GGATGATGTA 
GATAACGACG AAATTCATCA GCTTGGTTTG AGTAGAGACC AGGAATGGGG GATTACTCGG 
CTTTCTAGGG TTTCTTCTCA ATTTCTACCA CCAGATGGGT CAAGGGTTGT CAAGGTTCCG 
TCTTGTAACT ATGAATTGAG ATGCTCGTTT TTGTCTCAAA GAGGGTATTA TCCTGATGCT 
CTTGATAAGG CCAATCAAGA TAGTTTCGCT ATTCATACTC CTTTTGGGAG TAATTCGGAT 
GATCATTTCT TTGGGGTTTT TGATGGGCAT GGTGAGTTTG GTGCTCAGTG TTCTCAGTTT 
GTGAAGCGGA GGTTGTGTGA GAATCTTCTT AGACATGGTA GGTTTCGTGT GGATCCTGCT 
GAGGCTTGTA ATTCGGCTTT CTTGACCACG AATTCTCAGT TACATGCTGA TTTGGTAGAT 
GATAGTATGA GTGGGACTAC TGCGATTACG GTTATGGTTA GAGGAAGGAC GATATATGTG 
GCGAATGCAG GTGATTCCAG GGCGGTTTTA GCTGAGAAGA GGGATGGTGA TCTTGTAGCT 
GTTGATTTGT CTATTGACCA GACTCCTTTT AGACCTGATG AGCTTGAAAG GGTTAAGCTT 
TGTGGTGCTA GAGTTCTTAC TCTTGATCAG ATTGAAGGCT TGAAAAATCC TGATGTTCAG 
TGTTGGGGTA CTGAGGAAGA TGATGATGGA GACCCTCCGA GACTTTGGGT GCCTAATGGA 
ATGTATCCTG GAACTGCTTT TACCAGGAGT ATTGGTGATT CTATTGCAGA GACAATTGGT 
GTTGTTGCTA ACCCTGAGAT TGCTGTTGTT GAGCTCACAC CGGATAATCC TTTCTTTGTG 
GTTGCTAGTG ATGGCGTATT TGAGTTCATC TCTAGTCAAA CGGTGGTTGA CATGGTTGCA 
AAACATAAGG ATCCTCGAGA TGCTTGTGCT GCAATTGTTG CTGAATCATA TAGGCTATGG 
CTACAGTATG AAACTCGGAC AGATGATATA ACAATCATCG TCGTGCACAT TGATGGACTA 
AAAGATGATG CTCCTCGGCA ATTGTCGAGT ACAGGAACTC AATTGCAGCC TCCTATTCCA 
CAAGTTGTGG AGCTTACAGG TTCGGAATCT CCTTCCACCT TTGGATGGAA CTCTAAAAAT 
CAACGTGTGA GGCATGATCT ATCTCGAGCT AGGATACGTG CCATTGAGAA TTCACTAGAG 
AATGGACATG CTTGGGTTCC TCCATCTCCA GCCCACAGAA AAACCTGGGA AGAAGAAGCA 
CACATTGAGC GGGTGTTGCG TGACCATTTC CTCTTCAGGA AACTCACTGA TTCTCAGTGC 
CAGGTTTTGT TGGACTGCAT GCAAAGGCTT GAAGCTAATC CAGGGGATAT TGTTGTGAAA 
CAGGGTGGTG AAGGGGACTG CTTCTACGTT GTGGGTAGTG GAGAATTCGA GGTCTTAGCA 
ACTCAGAACG GTGAGGTACC TAGGATCTTG CAGCGTTATA CAGCTGAGAA ACAATCATCA 
TTCGGTGAAC TCGCCTTAAT GCATAACAAG CCGCTTCAGG CTTCTGTACG TGCTGTAGAT 
CACGGAACAT TGTGGGCCTT AAAAAGAGAA GATTTTCGAG GAATTTTGAT GTCTGAGTTT 
TCAAACTTAG CATCCTTGAA GCTTCTTCGT TCTGTCGATC TTCTTTCCCG TCTTACAATT 
TTGCAACTAA GCCATGTTGC AGAGTCTCTT TCCGAAGCTT GCTTTTCTGA TGGACAAACA 
ATTGTTACCA AGGACCAAAA ACTTCAGGGC CTGTATGTTA TCCAGAAGGG ACGCGTAAAA 
ATATCTTTCT GTACAGAGGT GTTGGAGAGT CAAAATGTTT CAAGCCTTAC AACTGGAATC 
ACTAACGAGT ATGACAATCT TGAAATCGGA ACAGAAGTCT CCATAGAAAA GCATGAAGGA 
AGTTACTTTG GTGAATGGGC TCTTCTTGGC GAACTCAAAG ATTCCTTAAG TGTGGTTGCA 
GTTGGCGAAG TGGTTTGTGT GGTTTTAACA AAAGAAAATT TCGAGTCAGC GGTCGGCCCT 
CTGACCAATA TTTCAGATGA CGGTCCCAAG ACAAGACATT CTTCATTCGA GCTGTCAAAG 
GAATCTGCAA AAGTTACCGA TACCACAGCT CTTGCTAAAG CCACTCTTGC AGACCTGGAA 
TGGACAACAT GCTTGAGTAC AACAGACTGT AGTGAGATTG GGCTCGTGCA TTTGAAAGAT 
AAAGAAAATT TGCTCAGCTT GAAAAGATTT TCAAAGCAAA AGGTGAAAAA GTTAGGTAAA 
GAGGCACAAG TATTGAAAGA GCGGAATCTG ATGAAGAACG TAATAAAGCC CTCAGCTATT 
GTTCCCGAAA TCTTGTGTAC TTGTGTCGAT CAAACATTTG CGGCAATCTT ACTGAACACT 
ACTCTTGCCT GTCCTATCTC TTCTCTGCTT CACTCTCCGC TTGACGAGTC ATCTGTCCGT 
TTCATTACCG GCTCACTTGT GTCTGCCATA GAAGACATAC ACAAGAACGA GATTCTCTTC 
AGAGGTTCAT CCCCCGAGTT ACTGATGTTG GATCAATCCG GATATCTACA GATTGTAGAC 
TTCAGATTCG CCAAGAAATT GTCCGGGGAA CGAACATTTA CAATCTGTGG AAATGCAGAT 
TATTTAGCCC CAGAAATTGT TCAAGGCAAA GGCCATGGTT ATGCAGCTGA CTGGTGGGCT 
CTTGGAGTTT TGATCTACTA TATGCTAGAA GGAGAAATGC CATTCGGGTC ATGGAGAGAG 
AGCGAGCTCG ACACGTTTCA GAAGATTGCA AAAGGCCAAC TAACTTTCCC TCGAGTTCTA 
AGCTCAGAAG CTGAAGATCT CATCACAAAG TTGCTTGAAG TTGATGAAAA CCTGCGGTTT 
GGTAGCCAAG GAGGTCCAGA ATCAATCAAG AAACATCCCT GGTTCAATGG TCTTAAATGG 
GAAGCTATTA GTAACCGCGA ATTTCAAGTT CCTCAAGAGA TAATCTCGCG AATTCATCAC 
CATTTGGAGA ATGATAACGT TCTCCCTCTA GAAACTTCTA AATCGCTTGA CACAACAGAA 
GATCAAGATG CTCAAAACTG GCTTGAAGAA TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Nature402(6763)761-8(1999 Dec)
Lin X,Kaul S,Rounsley S,Shea TP,Benito MI,Town CD,Fujii CY,Mason T,Bowman CL,Barnstead M,Feldblyum TV,Buell CR,Ketchum KA,Lee J,Ronning CM,Koo HL,Moffat KS,Cronin LA,Shen M,Pai G,Van Aken S,Umayam L,Tallon LJ,Gill JE,Adams MD,Carrera AJ,Creasy TH,Goodman HM,Somerville CR,Copenhaver GP,Preuss D,Nierman WC,White O,Eisen JA,Salzberg SL,Fraser CM,Venter JC

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AT2G20050 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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