AT4G20325 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT4G20325
Entrez Gene ID	5008148
Full Name	ribonuclease H2 subunit B
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001125548.1	NP_001119020.1	ribonuclease H2 subunit B
NM_001084943.2	NP_001078412.2	ribonuclease H2 subunit B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the identification of a second member of the RNase H2 complex, RNase H2B, being able to complement the root growth phenotype of WEE1(KO) plants, is reported.
Title: Lack of RNase H2 activity rescues HU-sensitivity of WEE1 deficient plants.

The following AT4G20325 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT4G20325 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb29118	NM_001084943.2 Latest version!	Arabidopsis thaliana ribonuclease H2 subunit B (AT4G20325), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OAb29119	NM_001125548.1 Latest version!	Arabidopsis thaliana ribonuclease H2 subunit B (AT4G20325), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT4G20325 ( NM_001084943.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001078412.2 Arabidopsis thaliana ribonuclease H2 subunit B (AT4G20325), mRNA.

Price & Availability↑

CloneID	OAb29118
Clone ID Related Accession (Same CDS sequence)	NM_001084943.2
Accession Version	NM_001084943.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	ribonuclease H2 subunit B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Apr 24, 2008 this sequence version replaced NM_001084943.1.

ATGAGTTCTA CTGTTTGGTG GGAAGGAGCT GAGAAGACAC GAGTTCTCAT AGCCCCAGAT 
TCAGGTTGTG GCGGAAACAA ACCCGGAGAA CTGTTAACAC TCCGCCATCC AAAATCAGAA 
AATGGAACAT GCTTCCTTTT TAATAATGAG ATGCTTCAAG AACTTCAGTG GTTTAAGCAA 
TCTTATGGTT CTTGGTTCTT GGGAGATTAC ATATCTGAAG ATGGAAGCTT ATATATGGCC 
ACTCCAGTTG ATCCTGTTTT CATATTGTTG CCTATTTTCG ACGAAGCAAG AATGAAGAAA 
GGTGAAAATT CTGGAAAGTT CAGGCAACTA GATGAGATTT TATTTGTGGA AGGATATCCT 
GGATATCAAC ATTTGTTGTC ACTTGCAGAG AAGTGTATGG AAATCGTTTG TCAAACTCAA 
GAGGTTGGAT CGATGAAGTT CTATCGGCTT GATAACTCGA AAGTCTTAGC TTGGTTAAGT 
TGTAAGATCT ACTGTTTAAA GAATTCTCTA CCGGAATTGG ACAAAAACTA TGCAGCTCAA 
GATGAGAAAC AAACATTGGT AGATTCCGTC TCAATTGTAG GAGAGTACCT GAAGACCGAG 
CCTTGGTTGA AGCTTCTATA TGATCATCTT GGACTCAAAT TTGTTGACCC GACGATGAAA 
GAAACCAATA TGGAGAATCT TCCAACTGCA AATGAGAATA ACATGGCATC ATCAAATTCA 
ATACAGGAGA AAGCGAATAA GAAGCCAGGA AAACAGACAA AGCAAGCAAA GGTAGAGACT 
GGATCAAAGA ACATAAGGGA TATGTTTTCA AGGGCTTGTA AGAAAAAATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001078412.2
CDS	185..1018
Translation	MSSTVWWEGAEKTRVLIAPDSGCGGNKPGELLTLRHPKSENGTCFLFNNEMLQELQWFKQSYGSWFLGDYISEDGSLYMATPVDPVFILLPIFDEARMKKGENSGKFRQLDEILFVEGYPGYQHLLSLAEKCMEIVCQTQEVGSMKFYRLDNSKVLAWLSCKIYCLKNSLPELDKNYAAQDEKQTLVDSVSIVGEYLKTEPWLKLLYDHLGLKFVDPTMKETNMENLPTANENNMASSNSIQEKANKKPGKQTKQAKVETGSKNIRDMFSRACKKKC

Target ORF information:

RefSeq Version	NM_001084943.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana ribonuclease H2 subunit B (AT4G20325), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001084943.2

ORF Insert Sequence:

ATGAGTTCTA CTGTTTGGTG GGAAGGAGCT GAGAAGACAC GAGTTCTCAT AGCCCCAGAT 
TCAGGTTGTG GCGGAAACAA ACCCGGAGAA CTGTTAACAC TCCGCCATCC AAAATCAGAA 
AATGGAACAT GCTTCCTTTT TAATAATGAG ATGCTTCAAG AACTTCAGTG GTTTAAGCAA 
TCTTATGGTT CTTGGTTCTT GGGAGATTAC ATATCTGAAG ATGGAAGCTT ATATATGGCC 
ACTCCAGTTG ATCCTGTTTT CATATTGTTG CCTATTTTCG ACGAAGCAAG AATGAAGAAA 
GGTGAAAATT CTGGAAAGTT CAGGCAACTA GATGAGATTT TATTTGTGGA AGGATATCCT 
GGATATCAAC ATTTGTTGTC ACTTGCAGAG AAGTGTATGG AAATCGTTTG TCAAACTCAA 
GAGGTTGGAT CGATGAAGTT CTATCGGCTT GATAACTCGA AAGTCTTAGC TTGGTTAAGT 
TGTAAGATCT ACTGTTTAAA GAATTCTCTA CCGGAATTGG ACAAAAACTA TGCAGCTCAA 
GATGAGAAAC AAACATTGGT AGATTCCGTC TCAATTGTAG GAGAGTACCT GAAGACCGAG 
CCTTGGTTGA AGCTTCTATA TGATCATCTT GGACTCAAAT TTGTTGACCC GACGATGAAA 
GAAACCAATA TGGAGAATCT TCCAACTGCA AATGAGAATA ACATGGCATC ATCAAATTCA 
ATACAGGAGA AAGCGAATAA GAAGCCAGGA AAACAGACAA AGCAAGCAAA GGTAGAGACT 
GGATCAAAGA ACATAAGGGA TATGTTTTCA AGGGCTTGTA AGAAAAAATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT4G20325 ( NM_001125548.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001119020.1 Arabidopsis thaliana ribonuclease H2 subunit B (AT4G20325), mRNA.

Price & Availability↑

CloneID	OAb29119
Clone ID Related Accession (Same CDS sequence)	NM_001125548.1
Accession Version	NM_001125548.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	ribonuclease H2 subunit B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGAGTTCTA CTGTTTGGTG GGAAGGAGCT GAGAAGACAC GAGTTCTCAT AGCCCCAGAT 
TCAGGTTGTG GCGGAAACAA ACCCGGAGAA CTGTTAACAC TCCGCCATCC AAAATCAGAA 
AATGGAACAT GCTTCCTTTT TAATAATGAG ATGCTTCAAG AACTTCAGTG GTTTAAGCAA 
TCTTATGGTT CTTGGTTCTT GGGAGATTAC ATATCTGAAG ATGGAAGCTT ATATATGGCC 
ACTCCAGTTG ATCCTGTTTT CATATTGTTG CCTATTTTCG ACGAAGCAAG AATGAAGAAA 
GGTGAAAATT CTGGAAAGTT CAGGCAACTA GATGAGATTT TATTTGTGGA AGGATATCCT 
GGATATCAAC ATTTGTTGTC ACTTGCAGAG AAGTGTATGG AAATCGTTTG TCAAACTCAA 
GAGGTTGGAT CGATGAAGTT CTATCGGCTT GATAACTCGA AAGTCTTAGC TTGGTTAAGT 
TGTAAGAGAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001119020.1
CDS	185..679
Translation	MSSTVWWEGAEKTRVLIAPDSGCGGNKPGELLTLRHPKSENGTCFLFNNEMLQELQWFKQSYGSWFLGDYISEDGSLYMATPVDPVFILLPIFDEARMKKGENSGKFRQLDEILFVEGYPGYQHLLSLAEKCMEIVCQTQEVGSMKFYRLDNSKVLAWLSCKRS

Target ORF information:

RefSeq Version	NM_001125548.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana ribonuclease H2 subunit B (AT4G20325), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001125548.1

ORF Insert Sequence:

ATGAGTTCTA CTGTTTGGTG GGAAGGAGCT GAGAAGACAC GAGTTCTCAT AGCCCCAGAT 
TCAGGTTGTG GCGGAAACAA ACCCGGAGAA CTGTTAACAC TCCGCCATCC AAAATCAGAA 
AATGGAACAT GCTTCCTTTT TAATAATGAG ATGCTTCAAG AACTTCAGTG GTTTAAGCAA 
TCTTATGGTT CTTGGTTCTT GGGAGATTAC ATATCTGAAG ATGGAAGCTT ATATATGGCC 
ACTCCAGTTG ATCCTGTTTT CATATTGTTG CCTATTTTCG ACGAAGCAAG AATGAAGAAA 
GGTGAAAATT CTGGAAAGTT CAGGCAACTA GATGAGATTT TATTTGTGGA AGGATATCCT 
GGATATCAAC ATTTGTTGTC ACTTGCAGAG AAGTGTATGG AAATCGTTTG TCAAACTCAA 
GAGGTTGGAT CGATGAAGTT CTATCGGCTT GATAACTCGA AAGTCTTAGC TTGGTTAAGT 
TGTAAGAGAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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AT4G20325 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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