EEFSEC cDNA ORF clone, Apteryx australis mantelli

Gene
Clones
gRNAs

Summary

Gene Symbol	EEFSEC
Entrez Gene ID	106500284
Full Name	eukaryotic elongation factor, selenocysteine-tRNA specific
Gene Type	protein-coding
Organism	Apteryx australis mantelli

mRNA and Protein(s)

mRNA	Protein	Name
XM_013962001.1	XP_013817455.1	selenocysteine-specific elongation factor

Pathways from BioSystems
Gene Ontology

The following EEFSEC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EEFSEC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAp11519	XM_013962001.1 Latest version!	Apteryx australis mantelli eukaryotic elongation factor, selenocysteine-tRNA-specific (EEFSEC), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EEFSEC ( XM_013962001.1 ) cDNA ORF clone, Apteryx australis mantelli -> XP_013817455.1 Apteryx australis mantelli eukaryotic elongation factor, selenocysteine-tRNA-specific (EEFSEC), mRNA.

Price & Availability↑

CloneID	OAp11519
Clone ID Related Accession (Same CDS sequence)	XM_013962001.1
Accession Version	XM_013962001.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-08
Organism	Apteryx australis mantelli
Product	selenocysteine-specific elongation factor
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013988969.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apteryx australis mantelli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTTG TAATAGATGT GACAAAAGGG ATGCAGACGC AGTCAGCAGA GTGCTTGGTA 
ATTGGGCAAA TCGCCTGCCA GAAGATGGTG GTAGTTCTCA ACAAAATAGA TCTCCTTCCA 
GAGGGCAAGA GGCAAAGTGC TATAGAGAAG ATGACTAAGA AAATGCAGAA GACATTGGAA 
AATACAAAGT TCTGTGGGTG TCCTATTGTT GCAGTTGCAG CAAAGCCTGG TGGACCGGAA 
GCCCCAGAGT CTGAGACTCC ACTAGGTGTT TCTGAATTAA TTGAGGTCCT CAAGTCTCAG 
GCTTACCTGC CATCAAGAGA CCCCTCGGGA CACTTTCTTA TGGCAGTCGA CCACTGTTTC 
TCTATCAAGG GTCAAGGCAC GGTGATGACA GGGACTATTC TTTCTGGCTC TGTTAGCCTT 
GGTGACAATG TGGAGATTCC TGCCCTGAAG GTGACAAAGA AAGTCAAGTC TATGCAGATG 
TTCCATACTC CTGTGACTTA TGCTATGCAG GGTGACAGAG TAGGCATCTG TGTGACCCAG 
TTTGATCCCA AACTGCTAGA ACGAGGTCTA ATTTGCACCC CAGAATCACT TCACACTATC 
CATGCTGCTA TCGTTTCCCT GAAGAAAATC CAGTATTTTC GAGGAGCTCT TCAGACAAAG 
GCCAAGTTTC ACATCACAGT TGGTCATGAA ACAGTCATGG GAAGAGTGAT GTTTTTCAGT 
CCAGCCCCTA CTGACTTCAA TGAAGAAATT CAAGAAAATG TTTTTAATTT TGAAAAAGAT 
TATCTTTATC AAGAGGAATA CTTGTCCAAG GACTCGAAGA CTTCAGAGGA GAACAAAGAA 
AATGACCAGT CAGGAGAAGT CCAGCTCCCA AGGCAACAGT GGGCTCTGCT AGAGTTTGAA 
AAACCAGTCA CTTGTCCGAA ACTGTGCCTT GTGATCGGCT CCAAGCTGGA TACGGATATT 
CATGCAAATA CCTGCAGATT GGCGTTCCAT GGGGTACTGC TGCAAGGTAT GGAAGACAAG 
AACTATGCAG AGACTTTCCT TCCTAAGCTG AAAGTGTACA AACTGAAGCA CAAAGAAGGG 
CAAGTGGAAA GGGTGATGGA TGATTACAGT GTGATTGGTC GTTCATTGTT TAAAAAGGAA 
ACGAACATCC AAATTTTTGT GGGTCTAAAA GTGAAACTAT CTACAGGAGA AGATGGAGTG 
ATAGAAGGTG GCTTTGGACA AAGCGGCAAA TTTAAAATCC GAATTCCAGA TGGGTTAAGG 
CCGGATACCA AGATGCTTCT CACTGTTGCC TCCAAGAAGA AGTCTAAGGC AAGCAAGGGT 
GACACAGCCA AAGAGGATGA AAGCAGCAAG AGTGATTCGG CCCAACCTGT TGCCATCACT 
CTTCTCTTTA AAAGATATGT CTTTGACATG CAGAAGAAAA TGATACAATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013817455.1
CDS	21..1454
Translation	MMLVIDVTKGMQTQSAECLVIGQIACQKMVVVLNKIDLLPEGKRQSAIEKMTKKMQKTLENTKFCGCPIVAVAAKPGGPEAPESETPLGVSELIEVLKSQAYLPSRDPSGHFLMAVDHCFSIKGQGTVMTGTILSGSVSLGDNVEIPALKVTKKVKSMQMFHTPVTYAMQGDRVGICVTQFDPKLLERGLICTPESLHTIHAAIVSLKKIQYFRGALQTKAKFHITVGHETVMGRVMFFSPAPTDFNEEIQENVFNFEKDYLYQEEYLSKDSKTSEENKENDQSGEVQLPRQQWALLEFEKPVTCPKLCLVIGSKLDTDIHANTCRLAFHGVLLQGMEDKNYAETFLPKLKVYKLKHKEGQVERVMDDYSVIGRSLFKKETNIQIFVGLKVKLSTGEDGVIEGGFGQSGKFKIRIPDGLRPDTKMLLTVASKKKSKASKGDTAKEDESSKSDSAQPVAITLLFKRYVFDMQKKMIQS

Target ORF information:

RefSeq Version	XM_013962001.1
Organism	Apteryx australis mantelli
Definition	Apteryx australis mantelli eukaryotic elongation factor, selenocysteine-tRNA-specific (EEFSEC), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013962001.1

ORF Insert Sequence:

ATGATGCTTG TAATAGATGT GACAAAAGGG ATGCAGACGC AGTCAGCAGA GTGCTTGGTA 
ATTGGGCAAA TCGCCTGCCA GAAGATGGTG GTAGTTCTCA ACAAAATAGA TCTCCTTCCA 
GAGGGCAAGA GGCAAAGTGC TATAGAGAAG ATGACTAAGA AAATGCAGAA GACATTGGAA 
AATACAAAGT TCTGTGGGTG TCCTATTGTT GCAGTTGCAG CAAAGCCTGG TGGACCGGAA 
GCCCCAGAGT CTGAGACTCC ACTAGGTGTT TCTGAATTAA TTGAGGTCCT CAAGTCTCAG 
GCTTACCTGC CATCAAGAGA CCCCTCGGGA CACTTTCTTA TGGCAGTCGA CCACTGTTTC 
TCTATCAAGG GTCAAGGCAC GGTGATGACA GGGACTATTC TTTCTGGCTC TGTTAGCCTT 
GGTGACAATG TGGAGATTCC TGCCCTGAAG GTGACAAAGA AAGTCAAGTC TATGCAGATG 
TTCCATACTC CTGTGACTTA TGCTATGCAG GGTGACAGAG TAGGCATCTG TGTGACCCAG 
TTTGATCCCA AACTGCTAGA ACGAGGTCTA ATTTGCACCC CAGAATCACT TCACACTATC 
CATGCTGCTA TCGTTTCCCT GAAGAAAATC CAGTATTTTC GAGGAGCTCT TCAGACAAAG 
GCCAAGTTTC ACATCACAGT TGGTCATGAA ACAGTCATGG GAAGAGTGAT GTTTTTCAGT 
CCAGCCCCTA CTGACTTCAA TGAAGAAATT CAAGAAAATG TTTTTAATTT TGAAAAAGAT 
TATCTTTATC AAGAGGAATA CTTGTCCAAG GACTCGAAGA CTTCAGAGGA GAACAAAGAA 
AATGACCAGT CAGGAGAAGT CCAGCTCCCA AGGCAACAGT GGGCTCTGCT AGAGTTTGAA 
AAACCAGTCA CTTGTCCGAA ACTGTGCCTT GTGATCGGCT CCAAGCTGGA TACGGATATT 
CATGCAAATA CCTGCAGATT GGCGTTCCAT GGGGTACTGC TGCAAGGTAT GGAAGACAAG 
AACTATGCAG AGACTTTCCT TCCTAAGCTG AAAGTGTACA AACTGAAGCA CAAAGAAGGG 
CAAGTGGAAA GGGTGATGGA TGATTACAGT GTGATTGGTC GTTCATTGTT TAAAAAGGAA 
ACGAACATCC AAATTTTTGT GGGTCTAAAA GTGAAACTAT CTACAGGAGA AGATGGAGTG 
ATAGAAGGTG GCTTTGGACA AAGCGGCAAA TTTAAAATCC GAATTCCAGA TGGGTTAAGG 
CCGGATACCA AGATGCTTCT CACTGTTGCC TCCAAGAAGA AGTCTAAGGC AAGCAAGGGT 
GACACAGCCA AAGAGGATGA AAGCAGCAAG AGTGATTCGG CCCAACCTGT TGCCATCACT 
CTTCTCTTTA AAAGATATGT CTTTGACATG CAGAAGAAAA TGATACAATC TTGA