EVC cDNA ORF clone, Apteryx australis mantelli

Gene
Clones
gRNAs

Summary

Gene Symbol	EVC
Entrez Gene ID	106498769
Full Name	EvC ciliary complex subunit 1
Gene Type	protein-coding
Organism	Apteryx australis mantelli

mRNA and Protein(s)

mRNA	Protein	Name
XM_013960095.1	XP_013815549.1	ellis-van Creveld syndrome protein isoform X1
XM_013960096.1	XP_013815550.1	ellis-van Creveld syndrome protein isoform X2

Pathways from BioSystems
Gene Ontology

The following EVC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EVC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAp27153	XM_013960095.1 Latest version!	Apteryx australis mantelli Ellis van Creveld protein (EVC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAp27154	XM_013960096.1 Latest version!	Apteryx australis mantelli Ellis van Creveld protein (EVC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EVC ( XM_013960095.1 ) cDNA ORF clone, Apteryx australis mantelli -> XP_013815549.1 Apteryx australis mantelli Ellis van Creveld protein (EVC), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAp27153
Clone ID Related Accession (Same CDS sequence)	XM_013960095.1
Accession Version	XM_013960095.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-08
Organism	Apteryx australis mantelli
Product	ellis-van Creveld syndrome protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014006397.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apteryx australis mantelli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTCGG AGGAAGATGA GTCTCCTCTG GACAGTAACG TCACAGCATT TGCATTAAAG 
GCAAAAGTCA TCTATCCAAT CAACCAGAAA TTTAGGCCAT TGGCAGATGG CTCATCCAAT 
CCGTCTTTGC ATGAGAATCC AAAGCAGGCT GTTCTGCCTA ATCAGGTAAT GGAGACGTCC 
ACTTCGGGCA GCCTGGGCTC CCTCAGCCAG GGAGACAAGG AGGACTGCAG TTCCTCCACG 
ACGATTCATT CCTCAGCCAG CGACGACAGG TTCCAGGCTC GGACCTTCCT CAAGGTGACC 
TGCTTCCCTG AAGTGCTGAC TTGCGAGAGT TTTGATGTTA AGCTCTGCCT CTGCAGTCTT 
CTTTTGAAAG ATCTTATGCT TCTTGATACT GAACTCAGAA AAGAAAAACA TGTGATTTTT 
ATTCAGGTTC TCAAAATATA CCTCACAGAT TTTTATCATA AAAAGAAGAT TACTGATGAT 
TTGTTCAAGA AGATCCTTTT AATTCAGGAA AATGATTTTG AAGAATTACA GAAACAACTT 
GACTCTAGAC TCCAGAATAC TGAGATGTCA GGAGCCCATA ATTCAGAGTA CCAGACCCTA 
GAAGACTTAG AAAGAAAAGA AAGAGAATAT TCTGAGCACA TAATAGATAA TATGGAAGCT 
TTCTGGAAGC AGGCTGACAA AGCCCAGCAA TTTCTTTTGG ACCAGTCAAA ATGCTCAAGT 
GCCAAAGCAA CAAAGATAAC TATGGATCTG ACTGAAAGGA TGATTGCAGT AGAAGGGTTA 
TTAAGTAAAT CACAGGATTT GCATGCAATG GACATTCAGG AAAGGTTATT TAACTGGGAG 
CTTATGGTAA AAGTGGTCGA TTCACTGAAG TCATATATAC CAGAAGAGTG CAAATGCAGA 
TTAAATACTG TATCAAATAT TCTGGACCAT TTAACTGTGA AGAATAATCT CTCAGTCCGA 
CAAAAGGAAG AGCTTTTAAC AGACCTGCAC AAGGCCTTCT GGGAACAACT AGCACATTTC 
AGTAATGAAT GTGTCCAGCA AAGTAAAGAC CTGATCCTGA AACAACTGGA GTACCATGCA 
AAGAAGAGAG AAGAGTTCAA ACACAGACAG AAAGCTGAAC AAGGGAGTCT CCTCAGTAAA 
ACTTTTCATA CTGAAGATTT TCATGATTTT CTTAAGGCTT ACCAGGAACT GTTAGAGAAG 
CATCGGCAGA TACAGTGGGA CCTGGAAGAG GAGGATGACT GTGAGAGCGC AGAGGCTGTT 
ACTGACCTCT ACAAGGAACT GTACTCTAAA GCTTCCCACG CTTTAGCGGA GTTGGTAAAG 
GAGCTGTTTC TTCAGACCCT CCCCGTTGTG ACCGGCCTCT CTGTAAGAGA ATGTGAACTT 
CTGAAAGAGG AATGGCAGGA GAATTTGGTC CCTCAGCTGG AGAAATGGGA CGTCTACCGC 
CAGAGACGCT GGAAGCTTTT CCAGGAACAG CTGCTGCAAG AGAAAAAGCT ATGGCTAAAG 
GAGTATGCCC TGTCTGCTGT AATACAAAAA CATCTGTCTG AGAAACAAGA GAAGATCATT 
CAAGGTGTCG TAAGCAGACT AGGAGGCCTC AGTGATGAGT CTGTGAACTA TATAATGCAA 
AAACACAAGC TTCTGCTGAG TTCAGTACTC AGAAGGGTAA GTCTCCGAAA CATTGGGATG 
GCTACTCTGA CCCGCATGAG GATGTCCAGG AAGAAGAGCT TGCTTCAGGA GCTGCGGGAG 
CAGCATGTCC TGCAGAAGAG CTCCTCCCAC TGCCAAGATG AAGATCAGTG GCAGCTACAG 
AAAGCAGTGG AGTCCCACAT TCTGGAAGAA GAAGAGAAGC TCGAGGAAGA AACACAGCAA 
ACACATTTAG AATTTCACCA GCAGTTAGTC ACCGAAATCC AAGAAGCTCT TCATTTTCTT 
CAGCAACACA TGGAGCAAGT GATCGGGCAG ACACTGCTGC AGCATGCCCG ACAGGAAGCT 
GCAAAAAAGA ATTCAGATGA TGGACAGGAC TGTAAGGAGA GGCTGGTGGA AATAGCTGTA 
GAAAGTGTGT ACGGGACCAG CAATAATATC AATAGACTCG TGCAAAACTA CTACCAACAA 
TTAGGAAAAA TCACAGAAGC TTATGAAGGG AAAAAACATC AACAATTGAA ATCCTTGCAA 
GAAAGAGGTG AAAGGAGCAG ACTTAGAAAG AAACAAGAGA ATGAACAGGC ATTGAAAGGA 
AAAGTGAACA AAGGCCTCCT GGACGTGTTG TCTGCAGTCC ATCAAAGGAT GGTGCTGCAG 
CAAGAGAAGG TTTTGGCTCA GTTTGAACAG CAGCAGCAGA TTCGTCTGGA GTCTCTGAAA 
CAGAAGATAC TGGGATTGCA TCAGCTAGAA ACTGAGTTGG AGAATCAGCT ACAGGAAGCA 
GAGCAGGACT TTGTCACAGA GCTAGCTACA CTGGCCCGCA TTCCACTCAC CGTGAAGAAG 
CAGCTTTCCA AAAGGAGCAA GCCAACAGGG GAAAAAACAA ACTCAAAAAG AAAGACCTTT 
CAATCTCCAG AATCAGAAGA CAAAGGTGAT TCTCATGAAA ATATTCAAGA GCCCCCTGGA 
CAGACTTCTG GCTTATACAG AGATAAATCG CAGAGTTCAC ATGAAGGGGA GACTGAGCCA 
AGGAAAAACT CAAAGATGCT GAAAAAAAGA GGCAACAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013815549.1
CDS	79..2760
Translation	MVSEEDESPLDSNVTAFALKAKVIYPINQKFRPLADGSSNPSLHENPKQAVLPNQVMETSTSGSLGSLSQGDKEDCSSSTTIHSSASDDRFQARTFLKVTCFPEVLTCESFDVKLCLCSLLLKDLMLLDTELRKEKHVIFIQVLKIYLTDFYHKKKITDDLFKKILLIQENDFEELQKQLDSRLQNTEMSGAHNSEYQTLEDLERKEREYSEHIIDNMEAFWKQADKAQQFLLDQSKCSSAKATKITMDLTERMIAVEGLLSKSQDLHAMDIQERLFNWELMVKVVDSLKSYIPEECKCRLNTVSNILDHLTVKNNLSVRQKEELLTDLHKAFWEQLAHFSNECVQQSKDLILKQLEYHAKKREEFKHRQKAEQGSLLSKTFHTEDFHDFLKAYQELLEKHRQIQWDLEEEDDCESAEAVTDLYKELYSKASHALAELVKELFLQTLPVVTGLSVRECELLKEEWQENLVPQLEKWDVYRQRRWKLFQEQLLQEKKLWLKEYALSAVIQKHLSEKQEKIIQGVVSRLGGLSDESVNYIMQKHKLLLSSVLRRVSLRNIGMATLTRMRMSRKKSLLQELREQHVLQKSSSHCQDEDQWQLQKAVESHILEEEEKLEEETQQTHLEFHQQLVTEIQEALHFLQQHMEQVIGQTLLQHARQEAAKKNSDDGQDCKERLVEIAVESVYGTSNNINRLVQNYYQQLGKITEAYEGKKHQQLKSLQERGERSRLRKKQENEQALKGKVNKGLLDVLSAVHQRMVLQQEKVLAQFEQQQQIRLESLKQKILGLHQLETELENQLQEAEQDFVTELATLARIPLTVKKQLSKRSKPTGEKTNSKRKTFQSPESEDKGDSHENIQEPPGQTSGLYRDKSQSSHEGETEPRKNSKMLKKRGNR

Target ORF information:

RefSeq Version	XM_013960095.1
Organism	Apteryx australis mantelli
Definition	Apteryx australis mantelli Ellis van Creveld protein (EVC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013960095.1

ORF Insert Sequence:

ATGGTGTCGG AGGAAGATGA GTCTCCTCTG GACAGTAACG TCACAGCATT TGCATTAAAG 
GCAAAAGTCA TCTATCCAAT CAACCAGAAA TTTAGGCCAT TGGCAGATGG CTCATCCAAT 
CCGTCTTTGC ATGAGAATCC AAAGCAGGCT GTTCTGCCTA ATCAGGTAAT GGAGACGTCC 
ACTTCGGGCA GCCTGGGCTC CCTCAGCCAG GGAGACAAGG AGGACTGCAG TTCCTCCACG 
ACGATTCATT CCTCAGCCAG CGACGACAGG TTCCAGGCTC GGACCTTCCT CAAGGTGACC 
TGCTTCCCTG AAGTGCTGAC TTGCGAGAGT TTTGATGTTA AGCTCTGCCT CTGCAGTCTT 
CTTTTGAAAG ATCTTATGCT TCTTGATACT GAACTCAGAA AAGAAAAACA TGTGATTTTT 
ATTCAGGTTC TCAAAATATA CCTCACAGAT TTTTATCATA AAAAGAAGAT TACTGATGAT 
TTGTTCAAGA AGATCCTTTT AATTCAGGAA AATGATTTTG AAGAATTACA GAAACAACTT 
GACTCTAGAC TCCAGAATAC TGAGATGTCA GGAGCCCATA ATTCAGAGTA CCAGACCCTA 
GAAGACTTAG AAAGAAAAGA AAGAGAATAT TCTGAGCACA TAATAGATAA TATGGAAGCT 
TTCTGGAAGC AGGCTGACAA AGCCCAGCAA TTTCTTTTGG ACCAGTCAAA ATGCTCAAGT 
GCCAAAGCAA CAAAGATAAC TATGGATCTG ACTGAAAGGA TGATTGCAGT AGAAGGGTTA 
TTAAGTAAAT CACAGGATTT GCATGCAATG GACATTCAGG AAAGGTTATT TAACTGGGAG 
CTTATGGTAA AAGTGGTCGA TTCACTGAAG TCATATATAC CAGAAGAGTG CAAATGCAGA 
TTAAATACTG TATCAAATAT TCTGGACCAT TTAACTGTGA AGAATAATCT CTCAGTCCGA 
CAAAAGGAAG AGCTTTTAAC AGACCTGCAC AAGGCCTTCT GGGAACAACT AGCACATTTC 
AGTAATGAAT GTGTCCAGCA AAGTAAAGAC CTGATCCTGA AACAACTGGA GTACCATGCA 
AAGAAGAGAG AAGAGTTCAA ACACAGACAG AAAGCTGAAC AAGGGAGTCT CCTCAGTAAA 
ACTTTTCATA CTGAAGATTT TCATGATTTT CTTAAGGCTT ACCAGGAACT GTTAGAGAAG 
CATCGGCAGA TACAGTGGGA CCTGGAAGAG GAGGATGACT GTGAGAGCGC AGAGGCTGTT 
ACTGACCTCT ACAAGGAACT GTACTCTAAA GCTTCCCACG CTTTAGCGGA GTTGGTAAAG 
GAGCTGTTTC TTCAGACCCT CCCCGTTGTG ACCGGCCTCT CTGTAAGAGA ATGTGAACTT 
CTGAAAGAGG AATGGCAGGA GAATTTGGTC CCTCAGCTGG AGAAATGGGA CGTCTACCGC 
CAGAGACGCT GGAAGCTTTT CCAGGAACAG CTGCTGCAAG AGAAAAAGCT ATGGCTAAAG 
GAGTATGCCC TGTCTGCTGT AATACAAAAA CATCTGTCTG AGAAACAAGA GAAGATCATT 
CAAGGTGTCG TAAGCAGACT AGGAGGCCTC AGTGATGAGT CTGTGAACTA TATAATGCAA 
AAACACAAGC TTCTGCTGAG TTCAGTACTC AGAAGGGTAA GTCTCCGAAA CATTGGGATG 
GCTACTCTGA CCCGCATGAG GATGTCCAGG AAGAAGAGCT TGCTTCAGGA GCTGCGGGAG 
CAGCATGTCC TGCAGAAGAG CTCCTCCCAC TGCCAAGATG AAGATCAGTG GCAGCTACAG 
AAAGCAGTGG AGTCCCACAT TCTGGAAGAA GAAGAGAAGC TCGAGGAAGA AACACAGCAA 
ACACATTTAG AATTTCACCA GCAGTTAGTC ACCGAAATCC AAGAAGCTCT TCATTTTCTT 
CAGCAACACA TGGAGCAAGT GATCGGGCAG ACACTGCTGC AGCATGCCCG ACAGGAAGCT 
GCAAAAAAGA ATTCAGATGA TGGACAGGAC TGTAAGGAGA GGCTGGTGGA AATAGCTGTA 
GAAAGTGTGT ACGGGACCAG CAATAATATC AATAGACTCG TGCAAAACTA CTACCAACAA 
TTAGGAAAAA TCACAGAAGC TTATGAAGGG AAAAAACATC AACAATTGAA ATCCTTGCAA 
GAAAGAGGTG AAAGGAGCAG ACTTAGAAAG AAACAAGAGA ATGAACAGGC ATTGAAAGGA 
AAAGTGAACA AAGGCCTCCT GGACGTGTTG TCTGCAGTCC ATCAAAGGAT GGTGCTGCAG 
CAAGAGAAGG TTTTGGCTCA GTTTGAACAG CAGCAGCAGA TTCGTCTGGA GTCTCTGAAA 
CAGAAGATAC TGGGATTGCA TCAGCTAGAA ACTGAGTTGG AGAATCAGCT ACAGGAAGCA 
GAGCAGGACT TTGTCACAGA GCTAGCTACA CTGGCCCGCA TTCCACTCAC CGTGAAGAAG 
CAGCTTTCCA AAAGGAGCAA GCCAACAGGG GAAAAAACAA ACTCAAAAAG AAAGACCTTT 
CAATCTCCAG AATCAGAAGA CAAAGGTGAT TCTCATGAAA ATATTCAAGA GCCCCCTGGA 
CAGACTTCTG GCTTATACAG AGATAAATCG CAGAGTTCAC ATGAAGGGGA GACTGAGCCA 
AGGAAAAACT CAAAGATGCT GAAAAAAAGA GGCAACAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EVC ( XM_013960096.1 ) cDNA ORF clone, Apteryx australis mantelli -> XP_013815550.1 Apteryx australis mantelli Ellis van Creveld protein (EVC), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAp27154
Clone ID Related Accession (Same CDS sequence)	XM_013960096.1
Accession Version	XM_013960096.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-08
Organism	Apteryx australis mantelli
Product	ellis-van Creveld syndrome protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014006397.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apteryx australis mantelli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTCGG AGGAAGATGA GTCTCCTCTG GACAGTAACG TCACAGCATT TGCATTAAAG 
GCAAAAGTCA TCTATCCAAT CAACCAGAAA TTTAGGCCAT TGGCAGATGG CTCATCCAAT 
CCGTCTTTGC ATGAGAATCC AAAGCAGGCT GTTCTGCCTA ATCAGGTAAT GGAGACGTCC 
ACTTCGGGCA GCCTGGGCTC CCTCAGCCAG GGAGACAAGG AGGACTGCAG TTCCTCCACG 
ACGATTCATT CCTCAGCCAG CGACGACAGG TTCCAGGCTC GGACCTTCCT CAAGGTGACC 
TGCTTCCCTG AAGTGCTGAC TTGCGAGAGT TTTGATGTTA AGCTCTGCCT CTGCAGTCTT 
CTTTTGAAAG ATCTTATGCT TCTTGATACT GAACTCAGAA AAGAAAAACA TGTGATTTTT 
ATTCAGGTTC TCAAAATATA CCTCACAGAT TTTTATCATA AAAAGAAGAT TACTGATGAT 
TTGTTCAAGA AGATCCTTTT AATTCAGGAA AATGATTTTG AAGAATTACA GAAACAACTT 
GACTCTAGAC TCCAGAATAC TGAGATGTCA GGAGCCCATA ATTCAGAGTA CCAGACCCTA 
GAAGACTTAG AAAGAAAAGA AAGAGAATAT TCTGAGCACA TAATAGATAA TATGGAAGCT 
TTCTGGAAGC AGGCTGACAA AGCCCAGCAA TTTCTTTTGG ACCAGTCAAA ATGCTCAAGT 
GCCAAAGCAA CAAAGATAAC TATGGATCTG ACTGAAAGGA TGATTGCAGT AGAAGGGTTA 
TTAAGTAAAT CACAGGATTT GCATGCAATG GACATTCAGG AAAGGTTATT TAACTGGGAG 
CTTATGGTAA AAGTGGTCGA TTCACTGAAG TCATATATAC CAGAAGAGTG CAAATGCAGA 
TTAAATACTG TATCAAATAT TCTGGACCAT TTAACTGTGA AGAATAATCT CTCAGTCCGA 
CAAAAGGAAG AGCTTTTAAC AGACCTGCAC AAGGCCTTCT GGGAACAACT AGCACATTTC 
AGTAATGAAT GTGTCCAGCA AAGTAAAGAC CTGATCCTGA AACAACTGGA GTACCATGCA 
AAGAAGAGAG AAGAGTTCAA ACACAGACAG AAAGCTGAAC AAGGGAGTCT CCTCAGTAAA 
ACTTTTCATA CTGAAGATTT TCATGATTTT CTTAAGGCTT ACCAGGAACT GTTAGAGAAG 
CATCGGCAGA TACAGTGGGA CCTGGAAGAG GAGGATGACT GTGAGAGCGC AGAGGCTGTT 
ACTGACCTCT ACAAGGAACT GTACTCTAAA GCTTCCCACG CTTTAGCGGA GTTGGTAAAG 
GAGCTGTTTC TTCAGACCCT CCCCGTTGTG ACCGGCCTCT CTGTAAGAGA ATGTGAACTT 
CTGAAAGAGG AATGGCAGGA GAATTTGGTC CCTCAGCTGG AGAAATGGGA CGTCTACCGC 
CAGAGACGCT GGAAGCTTTT CCAGGAACAG CTGCTGCAAG AGAAAAAGCT ATGGCTAAAG 
GAGTATGCCC TGTCTGCTGT AATACAAAAA CATCTGTCTG AGAAACAAGA GAAGATCATT 
CAAGGTGTCG TAAGCAGACT AGGAGGCCTC AGTGATGAGT CTGTGAACTA TATAATGCAA 
AAACACAAGC TTCTGCTGAG TTCAGTACTC AGAAGGGTAA GTCTCCGAAA CATTGGGATG 
GCTACTCTGA CCCGCATGAG GATGTCCAGG AAGAAGAGCT TGCTTCAGGA GCTGCGGGAG 
CAGCATGTCC TGCAGAAGAG CTCCTCCCAC TGCCAAGATG AAGATCAGTG GCAGCTACAG 
AAAGCAGTGG AGTCCCACAT TCTGGAAGAA GAAGAGAAGC TCGAGGAAGA AACACAGCAA 
ACACATTTAG AATTTCACCA GCAGTTAGTC ACCGAAATCC AAGAAGCTCT TCATTTTCTT 
CAGCAACACA TGGAGCAAGT GATCGGGCAG ACACTGCTGC AGCATGCCCG ACAGGAAGCT 
GCAAAAAAGA ATTCAGATGA TGGACAGGAC TGTAAGGAGA GGCTGGTGGA AATAGCTGTA 
GAAAGTGTGT ACGGGACCAG CAATAATATC AATAGACTCG TGCAAAACTA CTACCAACAA 
TTAGGAAAAA TCACAGAAGC TTATGAAGGG AAAAAACATC AACAATTGAA ATCCTTGCAA 
GCAGAAAGAG GTGAAAGGAG CAGACTTAGA AAGAAACAAG AGAATGAACA GGCATTGAAA 
GGAAAAGTGA ACAAAGGCCT CCTGGACGTG TTGTCTGCAG TCCATCAAAG GATGGTGCTG 
CAGCAAGAGA AGGTTTTGGC TCAGTTTGAA CAGCAGCAGC AGATTCGTCT GGAGTCTCTG 
AAACAGAAGA TACTGGGATT GCATCAGCTA GAAACTGAGT TGGAGAATCA GCTACAGGGG 
AAAAAACAAA CTCAAAAAGA AAGACCTTTC AATCTCCAGA ATCAGAAGAC AAAGGTGATT 
CTCATGAAAA TATTCAAGAG CCCCCTGGAC AGACTTCTGG CTTATACAGA GATAAATCGC 
AGAGTTCACA TGAAGGGGAG ACTGAGCCAA GGAAAAACTC AAAGATGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013815550.1
CDS	79..2649
Translation	MVSEEDESPLDSNVTAFALKAKVIYPINQKFRPLADGSSNPSLHENPKQAVLPNQVMETSTSGSLGSLSQGDKEDCSSSTTIHSSASDDRFQARTFLKVTCFPEVLTCESFDVKLCLCSLLLKDLMLLDTELRKEKHVIFIQVLKIYLTDFYHKKKITDDLFKKILLIQENDFEELQKQLDSRLQNTEMSGAHNSEYQTLEDLERKEREYSEHIIDNMEAFWKQADKAQQFLLDQSKCSSAKATKITMDLTERMIAVEGLLSKSQDLHAMDIQERLFNWELMVKVVDSLKSYIPEECKCRLNTVSNILDHLTVKNNLSVRQKEELLTDLHKAFWEQLAHFSNECVQQSKDLILKQLEYHAKKREEFKHRQKAEQGSLLSKTFHTEDFHDFLKAYQELLEKHRQIQWDLEEEDDCESAEAVTDLYKELYSKASHALAELVKELFLQTLPVVTGLSVRECELLKEEWQENLVPQLEKWDVYRQRRWKLFQEQLLQEKKLWLKEYALSAVIQKHLSEKQEKIIQGVVSRLGGLSDESVNYIMQKHKLLLSSVLRRVSLRNIGMATLTRMRMSRKKSLLQELREQHVLQKSSSHCQDEDQWQLQKAVESHILEEEEKLEEETQQTHLEFHQQLVTEIQEALHFLQQHMEQVIGQTLLQHARQEAAKKNSDDGQDCKERLVEIAVESVYGTSNNINRLVQNYYQQLGKITEAYEGKKHQQLKSLQAERGERSRLRKKQENEQALKGKVNKGLLDVLSAVHQRMVLQQEKVLAQFEQQQQIRLESLKQKILGLHQLETELENQLQGKKQTQKERPFNLQNQKTKVILMKIFKSPLDRLLAYTEINRRVHMKGRLSQGKTQRC

Target ORF information:

RefSeq Version	XM_013960096.1
Organism	Apteryx australis mantelli
Definition	Apteryx australis mantelli Ellis van Creveld protein (EVC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013960096.1

ORF Insert Sequence:

ATGGTGTCGG AGGAAGATGA GTCTCCTCTG GACAGTAACG TCACAGCATT TGCATTAAAG 
GCAAAAGTCA TCTATCCAAT CAACCAGAAA TTTAGGCCAT TGGCAGATGG CTCATCCAAT 
CCGTCTTTGC ATGAGAATCC AAAGCAGGCT GTTCTGCCTA ATCAGGTAAT GGAGACGTCC 
ACTTCGGGCA GCCTGGGCTC CCTCAGCCAG GGAGACAAGG AGGACTGCAG TTCCTCCACG 
ACGATTCATT CCTCAGCCAG CGACGACAGG TTCCAGGCTC GGACCTTCCT CAAGGTGACC 
TGCTTCCCTG AAGTGCTGAC TTGCGAGAGT TTTGATGTTA AGCTCTGCCT CTGCAGTCTT 
CTTTTGAAAG ATCTTATGCT TCTTGATACT GAACTCAGAA AAGAAAAACA TGTGATTTTT 
ATTCAGGTTC TCAAAATATA CCTCACAGAT TTTTATCATA AAAAGAAGAT TACTGATGAT 
TTGTTCAAGA AGATCCTTTT AATTCAGGAA AATGATTTTG AAGAATTACA GAAACAACTT 
GACTCTAGAC TCCAGAATAC TGAGATGTCA GGAGCCCATA ATTCAGAGTA CCAGACCCTA 
GAAGACTTAG AAAGAAAAGA AAGAGAATAT TCTGAGCACA TAATAGATAA TATGGAAGCT 
TTCTGGAAGC AGGCTGACAA AGCCCAGCAA TTTCTTTTGG ACCAGTCAAA ATGCTCAAGT 
GCCAAAGCAA CAAAGATAAC TATGGATCTG ACTGAAAGGA TGATTGCAGT AGAAGGGTTA 
TTAAGTAAAT CACAGGATTT GCATGCAATG GACATTCAGG AAAGGTTATT TAACTGGGAG 
CTTATGGTAA AAGTGGTCGA TTCACTGAAG TCATATATAC CAGAAGAGTG CAAATGCAGA 
TTAAATACTG TATCAAATAT TCTGGACCAT TTAACTGTGA AGAATAATCT CTCAGTCCGA 
CAAAAGGAAG AGCTTTTAAC AGACCTGCAC AAGGCCTTCT GGGAACAACT AGCACATTTC 
AGTAATGAAT GTGTCCAGCA AAGTAAAGAC CTGATCCTGA AACAACTGGA GTACCATGCA 
AAGAAGAGAG AAGAGTTCAA ACACAGACAG AAAGCTGAAC AAGGGAGTCT CCTCAGTAAA 
ACTTTTCATA CTGAAGATTT TCATGATTTT CTTAAGGCTT ACCAGGAACT GTTAGAGAAG 
CATCGGCAGA TACAGTGGGA CCTGGAAGAG GAGGATGACT GTGAGAGCGC AGAGGCTGTT 
ACTGACCTCT ACAAGGAACT GTACTCTAAA GCTTCCCACG CTTTAGCGGA GTTGGTAAAG 
GAGCTGTTTC TTCAGACCCT CCCCGTTGTG ACCGGCCTCT CTGTAAGAGA ATGTGAACTT 
CTGAAAGAGG AATGGCAGGA GAATTTGGTC CCTCAGCTGG AGAAATGGGA CGTCTACCGC 
CAGAGACGCT GGAAGCTTTT CCAGGAACAG CTGCTGCAAG AGAAAAAGCT ATGGCTAAAG 
GAGTATGCCC TGTCTGCTGT AATACAAAAA CATCTGTCTG AGAAACAAGA GAAGATCATT 
CAAGGTGTCG TAAGCAGACT AGGAGGCCTC AGTGATGAGT CTGTGAACTA TATAATGCAA 
AAACACAAGC TTCTGCTGAG TTCAGTACTC AGAAGGGTAA GTCTCCGAAA CATTGGGATG 
GCTACTCTGA CCCGCATGAG GATGTCCAGG AAGAAGAGCT TGCTTCAGGA GCTGCGGGAG 
CAGCATGTCC TGCAGAAGAG CTCCTCCCAC TGCCAAGATG AAGATCAGTG GCAGCTACAG 
AAAGCAGTGG AGTCCCACAT TCTGGAAGAA GAAGAGAAGC TCGAGGAAGA AACACAGCAA 
ACACATTTAG AATTTCACCA GCAGTTAGTC ACCGAAATCC AAGAAGCTCT TCATTTTCTT 
CAGCAACACA TGGAGCAAGT GATCGGGCAG ACACTGCTGC AGCATGCCCG ACAGGAAGCT 
GCAAAAAAGA ATTCAGATGA TGGACAGGAC TGTAAGGAGA GGCTGGTGGA AATAGCTGTA 
GAAAGTGTGT ACGGGACCAG CAATAATATC AATAGACTCG TGCAAAACTA CTACCAACAA 
TTAGGAAAAA TCACAGAAGC TTATGAAGGG AAAAAACATC AACAATTGAA ATCCTTGCAA 
GCAGAAAGAG GTGAAAGGAG CAGACTTAGA AAGAAACAAG AGAATGAACA GGCATTGAAA 
GGAAAAGTGA ACAAAGGCCT CCTGGACGTG TTGTCTGCAG TCCATCAAAG GATGGTGCTG 
CAGCAAGAGA AGGTTTTGGC TCAGTTTGAA CAGCAGCAGC AGATTCGTCT GGAGTCTCTG 
AAACAGAAGA TACTGGGATT GCATCAGCTA GAAACTGAGT TGGAGAATCA GCTACAGGGG 
AAAAAACAAA CTCAAAAAGA AAGACCTTTC AATCTCCAGA ATCAGAAGAC AAAGGTGATT 
CTCATGAAAA TATTCAAGAG CCCCCTGGAC AGACTTCTGG CTTATACAGA GATAAATCGC 
AGAGTTCACA TGAAGGGGAG ACTGAGCCAA GGAAAAACTC AAAGATGCTG A