CHRNA3 cDNA ORF clone, Apteryx australis mantelli

Gene
Clones
gRNAs

Summary

Gene Symbol	CHRNA3
Entrez Gene ID	106496367
Full Name	cholinergic receptor nicotinic alpha 3 subunit
Gene Type	protein-coding
Organism	Apteryx australis mantelli

mRNA and Protein(s)

mRNA	Protein	Name
XM_013957009.1	XP_013812463.1	LOW QUALITY PROTEIN: neuronal acetylcholine receptor subunit alpha-3

Pathways from BioSystems
Gene Ontology

The following CHRNA3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNA3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAp24817	XM_013957009.1 Latest version!	Apteryx australis mantelli cholinergic receptor, nicotinic, alpha 3 (neuronal) (CHRNA3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHRNA3 ( XM_013957009.1 ) cDNA ORF clone, Apteryx australis mantelli -> XP_013812463.1 Apteryx australis mantelli cholinergic receptor, nicotinic, alpha 3 (neuronal) (CHRNA3), mRNA.

Price & Availability↑

CloneID	OAp24817
Clone ID Related Accession (Same CDS sequence)	XM_013957009.1
Accession Version	XM_013957009.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-08
Organism	Apteryx australis mantelli
Product	LOW QUALITY PROTEIN: neuronal acetylcholine receptor subunit alpha-3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014005022.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apteryx australis mantelli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

ATGGGGAGCT CGCACACTCT CCTCCTAACT GCTGCCGTCT GCTTTCTCTT TAAAGAATCG 
CAATTTCTTC CCAACCTAAC GCGGACTCGC TTCTTGCGCC ATTAGATCTG GAATGATTAT 
AAGCTTCGCT GGAATCCAGT GGACTATGGA GGTGCTGAAT TCATCCGAGT GCCATCTGGC 
CAGATCTGGA AGCCAGATAT TGTTTTATAT AACAATGCAG TTGGAGATTT CCAGGTTGAT 
GACAAGACAA AGGCCCTACT GAAATACACA GGTGATGTGA CCTGGATACC TCCGGCTATA 
TTTAAAAGCT CGTGTAAAAT AGATGTAACC TACTTCCCTT TTGACTATCA GAACTGCACC 
ATGAAGTTTG GTTCCTGGTC TTATGATAAA GCCAAAATTG ACTTAGTTTT AATTGGCTCT 
ACAATGAACC TGAAAGATTA CTGGGAGAGT GGAGAATGGG CTATTATTAA AGCTCCTGGG 
TATAAACATG ACATCAAATA CAACTGTTGT GAAGAGATAT ATCCAGACAT CACATACTCT 
CTTTACATTA GGCGTTTACC TTTATTTTAC ACTATCAACA TGATTATTCC ATGTCTGCTG 
ATTTCTTTTC TGACTGTCCT GGTTTTCTAC TTGCCTTCAG ATTGTGGTGA GAAGGTGACA 
CTCTGCATAT CGGTCCTTTT ATCTTTAACA GTGTTCCTTC TTGTTATCAC AGAAACCATA 
CCTTCTACTT CTCTGGTAAT TCCACTTATT GGGGAATACC TCCTTTTCAC CATGATATTT 
GTAACTCTAT CTATTGTCAT TACGGTATTT GTACTTAACG TGCACTACAG AACACCCAAG 
ACGCACACAA TGCCCGTGTG GGTGAGAACC ATCTTCTTGA ACTTACTTCC CAGGATCATG 
TTTATGACAA GGCCTGCCAG TGATGAAGAG AATAATCAGA AGCCAAAACC ATTTTACACT 
GCTGAGTTTT CAAACTTAAA CTGCTTCAAC AGTTCTGAAA CCAAATGCTG CAAAGATGGC 
TTTGTATGTC AAGATACGGC ATGCAGCTGT TGTCAGCATC AACGAATGAA GTTCTCAGAT 
TTCAGTGGCA ATCTCACAAG AAGTTCAAGC TCTGAATCTG TAGATCCTCT GCTTTCATTT 
TCAGTTCTGT CACCAGAAAT GAGAGATGCT ATTGAAAGTG TTAAATACAT TGCAGAAAAT 
ATGAAAATGC AGAATGAAGC AAAAGAGATT CAGGATGACT GGAAATACGT TGCCATGGTA 
ATTGATCGCA TTTTTCTATG GGTTTTCATC CTGGTATGTA TTCTAGGAAC AGCAGGATTG 
TTTTTACAAC CTTTGATGGC TGGAGATGAA ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013812463.1
CDS	181..1536
Translation	MGSSHTLLLTAAVCFLFKESQFLPNLTRTRFLRH*IWNDYKLRWNPVDYGGAEFIRVPSGQIWKPDIVLYNNAVGDFQVDDKTKALLKYTGDVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSTMNLKDYWESGEWAIIKAPGYKHDIKYNCCEEIYPDITYSLYIRRLPLFYTINMIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPKTHTMPVWVRTIFLNLLPRIMFMTRPASDEENNQKPKPFYTAEFSNLNCFNSSETKCCKDGFVCQDTACSCCQHQRMKFSDFSGNLTRSSSSESVDPLLSFSVLSPEMRDAIESVKYIAENMKMQNEAKEIQDDWKYVAMVIDRIFLWVFILVCILGTAGLFLQPLMAGDEM

Target ORF information:

RefSeq Version	XM_013957009.1
Organism	Apteryx australis mantelli
Definition	Apteryx australis mantelli cholinergic receptor, nicotinic, alpha 3 (neuronal) (CHRNA3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013957009.1

ORF Insert Sequence:

ATGGGGAGCT CGCACACTCT CCTCCTAACT GCTGCCGTCT GCTTTCTCTT TAAAGAATCG 
CAATTTCTTC CCAACCTAAC GCGGACTCGC TTCTTGCGCC ATTAGATCTG GAATGATTAT 
AAGCTTCGCT GGAATCCAGT GGACTATGGA GGTGCTGAAT TCATCCGAGT GCCATCTGGC 
CAGATCTGGA AGCCAGATAT TGTTTTATAT AACAATGCAG TTGGAGATTT CCAGGTTGAT 
GACAAGACAA AGGCCCTACT GAAATACACA GGTGATGTGA CCTGGATACC TCCGGCTATA 
TTTAAAAGCT CGTGTAAAAT AGATGTAACC TACTTCCCTT TTGACTATCA GAACTGCACC 
ATGAAGTTTG GTTCCTGGTC TTATGATAAA GCCAAAATTG ACTTAGTTTT AATTGGCTCT 
ACAATGAACC TGAAAGATTA CTGGGAGAGT GGAGAATGGG CTATTATTAA AGCTCCTGGG 
TATAAACATG ACATCAAATA CAACTGTTGT GAAGAGATAT ATCCAGACAT CACATACTCT 
CTTTACATTA GGCGTTTACC TTTATTTTAC ACTATCAACA TGATTATTCC ATGTCTGCTG 
ATTTCTTTTC TGACTGTCCT GGTTTTCTAC TTGCCTTCAG ATTGTGGTGA GAAGGTGACA 
CTCTGCATAT CGGTCCTTTT ATCTTTAACA GTGTTCCTTC TTGTTATCAC AGAAACCATA 
CCTTCTACTT CTCTGGTAAT TCCACTTATT GGGGAATACC TCCTTTTCAC CATGATATTT 
GTAACTCTAT CTATTGTCAT TACGGTATTT GTACTTAACG TGCACTACAG AACACCCAAG 
ACGCACACAA TGCCCGTGTG GGTGAGAACC ATCTTCTTGA ACTTACTTCC CAGGATCATG 
TTTATGACAA GGCCTGCCAG TGATGAAGAG AATAATCAGA AGCCAAAACC ATTTTACACT 
GCTGAGTTTT CAAACTTAAA CTGCTTCAAC AGTTCTGAAA CCAAATGCTG CAAAGATGGC 
TTTGTATGTC AAGATACGGC ATGCAGCTGT TGTCAGCATC AACGAATGAA GTTCTCAGAT 
TTCAGTGGCA ATCTCACAAG AAGTTCAAGC TCTGAATCTG TAGATCCTCT GCTTTCATTT 
TCAGTTCTGT CACCAGAAAT GAGAGATGCT ATTGAAAGTG TTAAATACAT TGCAGAAAAT 
ATGAAAATGC AGAATGAAGC AAAAGAGATT CAGGATGACT GGAAATACGT TGCCATGGTA 
ATTGATCGCA TTTTTCTATG GGTTTTCATC CTGGTATGTA TTCTAGGAAC AGCAGGATTG 
TTTTTACAAC CTTTGATGGC TGGAGATGAA ATGTAA