GNE cDNA ORF clone, Apteryx australis mantelli

Gene
Clones
gRNAs

Summary

Gene Symbol	GNE
Entrez Gene ID	106487477
Full Name	glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Gene Type	protein-coding
Organism	Apteryx australis mantelli

mRNA and Protein(s)

mRNA	Protein	Name
XM_013946243.1	XP_013801697.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase

Pathways from BioSystems
Gene Ontology

The following GNE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAp16796	XM_013946243.1 Latest version!	Apteryx australis mantelli glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GNE ( XM_013946243.1 ) cDNA ORF clone, Apteryx australis mantelli -> XP_013801697.1 Apteryx australis mantelli glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), mRNA.

Price & Availability↑

CloneID	OAp16796
Clone ID Related Accession (Same CDS sequence)	XM_013946243.1
Accession Version	XM_013946243.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-08
Organism	Apteryx australis mantelli
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013994825.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apteryx australis mantelli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA ATGGAAGCAA CCACAAACTT CGTGTTTGTG TTGCTACTTG CAATCGGGCT 
GATTATTCAA AATTAGCTCC CATTATGTTT GGTATTAAGG CAGAACCACA GTTCTTTGAG 
CTTGATGTTG TAGTGCTTGG TTCCCATCTG ATTGATGATT ATGGTAACAC CTATCGAATG 
ATTGAACAAG ATGACTTTGA CATTCATACA AGGTTGCACA CTATTGTGAG AGGCGAGGAT 
GAGGCAGCCA TGGTGGAGTC AGTGGGCCTT GCATTGGTGA AGTTACCAGA TGTCCTGAAC 
CGCTTGAAAC CTGACATAAT GATAGTGCAT GGTGACAGAT TTGATGCTTT GGCACTGGCC 
ACTTCTGCAG CCCTGATGAA TATTCGAATT CTTCACATTG AAGGTGGGGA AGTCAGCGGG 
ACCATAGATG ACTCTATCAG ACACGCAATC ACCAAACTGG CCCATTACCA CGTATGCTGC 
ACAAGGAGTG CAGAGCAGCA TTTGATAGCC ATGTGTGAAG ACCACGATCG CATCCTTTTA 
GCGGGATGCC CCTCATATGA CAAGCTTCTG TCTGCCAAAA ATAAGGACTA CATGAGTATT 
ATACGCATAT GGCTAGGTGA AGATGTCAAA TCCAGAGATT ATATAGTTGC TCTGCAGCAT 
CCTGTAACCA CAGATATTAA GCATTCCATA AAGATGTTTG AGCTGACGCT AGATGCACTC 
ATCTCCTTCA ACAAGAGAAC GCTTGTTCTC TTCCCCAACG TGGATGCAGG AAGCAAAGAG 
ATGGTTCGGG TGATGAGGAA GAAGGGCATT GAACACCATC CCAATTTTCG GGCAGTAAAG 
CATGTTCCAT TTGACCAGTT CATTCAGCTA GTGGCGCACG CTGGTTGTAT GATCGGTAAC 
AGCAGCTGTG GTGTTAGAGA GGTGGGAGCA TTTGGCACAC CTGTCATCAA CCTGGGGACA 
CGTCAGACAG GAAGAGAAAC AGGTGAAAAT GTTCTTCATG TTCGTGATGC TGACACACAG 
GATAAGATTC TGCATGCTCT GCAGCTTCAG TTCGGTAAAC AGTATCCATG CTCAAAAATA 
TATGGAGATG GTAATGCCGT TCCAAGGATT CTGAAGTTCC TCAAATCTAT TGACCTCGAA 
GAGCCACTGC AAAAGAAATT CTGTTTTCCT CCTGTCAAAG ATAATATCTC TCAAGATATC 
GACCATATTC TAGAGACACA GAGTGCCTTG GCAGTGGATC TAGGTGGAAC AAATCTCCGA 
GTAGCAATTG TCAGTATGAA GGGTGAAATA GTTAAGAAGT ATACCCAGCT CAACCCTAAG 
ACATATGAAG ACAGACTAGA ATTAATTCTG AAGCTTTACT TCAAGGATTG CACACACTTA 
GTTTTGCTTT CTCTCCTGTG TTTATGGGAC TTAGGTATTT CCACAGGTGG ACGGGTAAAT 
CCCCGAGAAG GAATTGTGCT TCACTCTACC AAGCTCATTC AGGAGTGGAG CTGTGTGGAT 
CTCAGAACCC CAATATCTGA TGCTCTGCAC CTGCCCGTCT GGGTGGACAA TGATGGAAAC 
TGTGCTGCTT TGGCAGAGAG GAAATTTGGT CATGGAAAAG GAGTAGAAAA TTTTGTAACA 
CTCATCACTG GTACAGGAAT TGGAGGTGGA ATCATTCATC AACATGAACT GATCCACGGC 
AGTTCTTTCT GTGCTGCTGA ACTTGGGCAT ATTGTCGTGT CTTTAGATGG GCCAGAGTGT 
CTCTGTGGTA GCCAAGGATG TATAGAAGCA TATGCCTCTG GAATAGCCTT ACAGAGAGAA 
GCTAAGAAAC TGCATGATGA GGATCTGCTT TTAGTAGAAG GAATGTCCAT GAAGAAGGAG 
GAGGTTGTTA GTGCTGCACA TCTCATTCAA GCAGCTAAAC TTGGAAATGC AAAAGCAGAC 
AGCATTCTCA GAACAGCTGG GACAGCATTA GGCCTTGGAG TTGTGAACAT TCTGCACACC 
ATGAACCCCT CTCTTGTGAT CCTGTCTGGA ATTCTAGCCA GCCACTATGT TAATGCTGTT 
AAAGATGTGA TACAGCGACA GGCTTTGTCC TCTGCTAAAA CTGTGGATGT GGTGGTCTCA 
AATCTAGCAG ATCCTGCTCT TCTTGGAGCT GCTAGCCTGG TACTGGATTA CACTACACGT 
AGAACATACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013801697.1
CDS	40..2211
Translation	MEKNGSNHKLRVCVATCNRADYSKLAPIMFGIKAEPQFFELDVVVLGSHLIDDYGNTYRMIEQDDFDIHTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLIAMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRIWLGEDVKSRDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNVDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQTGRETGENVLHVRDADTQDKILHALQLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLEEPLQKKFCFPPVKDNISQDIDHILETQSALAVDLGGTNLRVAIVSMKGEIVKKYTQLNPKTYEDRLELILKLYFKDCTHLVLLSLLCLWDLGISTGGRVNPREGIVLHSTKLIQEWSCVDLRTPISDALHLPVWVDNDGNCAALAERKFGHGKGVENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHIVVSLDGPECLCGSQGCIEAYASGIALQREAKKLHDEDLLLVEGMSMKKEEVVSAAHLIQAAKLGNAKADSILRTAGTALGLGVVNILHTMNPSLVILSGILASHYVNAVKDVIQRQALSSAKTVDVVVSNLADPALLGAASLVLDYTTRRTY

Target ORF information:

RefSeq Version	XM_013946243.1
Organism	Apteryx australis mantelli
Definition	Apteryx australis mantelli glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013946243.1

ORF Insert Sequence:

ATGGAGAAGA ATGGAAGCAA CCACAAACTT CGTGTTTGTG TTGCTACTTG CAATCGGGCT 
GATTATTCAA AATTAGCTCC CATTATGTTT GGTATTAAGG CAGAACCACA GTTCTTTGAG 
CTTGATGTTG TAGTGCTTGG TTCCCATCTG ATTGATGATT ATGGTAACAC CTATCGAATG 
ATTGAACAAG ATGACTTTGA CATTCATACA AGGTTGCACA CTATTGTGAG AGGCGAGGAT 
GAGGCAGCCA TGGTGGAGTC AGTGGGCCTT GCATTGGTGA AGTTACCAGA TGTCCTGAAC 
CGCTTGAAAC CTGACATAAT GATAGTGCAT GGTGACAGAT TTGATGCTTT GGCACTGGCC 
ACTTCTGCAG CCCTGATGAA TATTCGAATT CTTCACATTG AAGGTGGGGA AGTCAGCGGG 
ACCATAGATG ACTCTATCAG ACACGCAATC ACCAAACTGG CCCATTACCA CGTATGCTGC 
ACAAGGAGTG CAGAGCAGCA TTTGATAGCC ATGTGTGAAG ACCACGATCG CATCCTTTTA 
GCGGGATGCC CCTCATATGA CAAGCTTCTG TCTGCCAAAA ATAAGGACTA CATGAGTATT 
ATACGCATAT GGCTAGGTGA AGATGTCAAA TCCAGAGATT ATATAGTTGC TCTGCAGCAT 
CCTGTAACCA CAGATATTAA GCATTCCATA AAGATGTTTG AGCTGACGCT AGATGCACTC 
ATCTCCTTCA ACAAGAGAAC GCTTGTTCTC TTCCCCAACG TGGATGCAGG AAGCAAAGAG 
ATGGTTCGGG TGATGAGGAA GAAGGGCATT GAACACCATC CCAATTTTCG GGCAGTAAAG 
CATGTTCCAT TTGACCAGTT CATTCAGCTA GTGGCGCACG CTGGTTGTAT GATCGGTAAC 
AGCAGCTGTG GTGTTAGAGA GGTGGGAGCA TTTGGCACAC CTGTCATCAA CCTGGGGACA 
CGTCAGACAG GAAGAGAAAC AGGTGAAAAT GTTCTTCATG TTCGTGATGC TGACACACAG 
GATAAGATTC TGCATGCTCT GCAGCTTCAG TTCGGTAAAC AGTATCCATG CTCAAAAATA 
TATGGAGATG GTAATGCCGT TCCAAGGATT CTGAAGTTCC TCAAATCTAT TGACCTCGAA 
GAGCCACTGC AAAAGAAATT CTGTTTTCCT CCTGTCAAAG ATAATATCTC TCAAGATATC 
GACCATATTC TAGAGACACA GAGTGCCTTG GCAGTGGATC TAGGTGGAAC AAATCTCCGA 
GTAGCAATTG TCAGTATGAA GGGTGAAATA GTTAAGAAGT ATACCCAGCT CAACCCTAAG 
ACATATGAAG ACAGACTAGA ATTAATTCTG AAGCTTTACT TCAAGGATTG CACACACTTA 
GTTTTGCTTT CTCTCCTGTG TTTATGGGAC TTAGGTATTT CCACAGGTGG ACGGGTAAAT 
CCCCGAGAAG GAATTGTGCT TCACTCTACC AAGCTCATTC AGGAGTGGAG CTGTGTGGAT 
CTCAGAACCC CAATATCTGA TGCTCTGCAC CTGCCCGTCT GGGTGGACAA TGATGGAAAC 
TGTGCTGCTT TGGCAGAGAG GAAATTTGGT CATGGAAAAG GAGTAGAAAA TTTTGTAACA 
CTCATCACTG GTACAGGAAT TGGAGGTGGA ATCATTCATC AACATGAACT GATCCACGGC 
AGTTCTTTCT GTGCTGCTGA ACTTGGGCAT ATTGTCGTGT CTTTAGATGG GCCAGAGTGT 
CTCTGTGGTA GCCAAGGATG TATAGAAGCA TATGCCTCTG GAATAGCCTT ACAGAGAGAA 
GCTAAGAAAC TGCATGATGA GGATCTGCTT TTAGTAGAAG GAATGTCCAT GAAGAAGGAG 
GAGGTTGTTA GTGCTGCACA TCTCATTCAA GCAGCTAAAC TTGGAAATGC AAAAGCAGAC 
AGCATTCTCA GAACAGCTGG GACAGCATTA GGCCTTGGAG TTGTGAACAT TCTGCACACC 
ATGAACCCCT CTCTTGTGAT CCTGTCTGGA ATTCTAGCCA GCCACTATGT TAATGCTGTT 
AAAGATGTGA TACAGCGACA GGCTTTGTCC TCTGCTAAAA CTGTGGATGT GGTGGTCTCA 
AATCTAGCAG ATCCTGCTCT TCTTGGAGCT GCTAGCCTGG TACTGGATTA CACTACACGT 
AGAACATACT AG