LOC101863543 cDNA ORF clone, Aplysia californica(California sea hare)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101863543
Entrez Gene ID	101863543
Full Name	fibroin heavy chain-like
Gene Type	protein-coding
Organism	Aplysia californica(California sea hare)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013088374.1	XP_012943828.1	fibroin heavy chain-like isoform X1
XM_013088375.1	XP_012943829.1	ribonucleoprotein PTB-binding 1-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC101863543 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101863543 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAp160532	XM_013088375.1 Latest version!	Aplysia californica fibroin heavy chain-like (LOC101863543), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OAp160531	XM_013088374.1 Latest version!	Aplysia californica fibroin heavy chain-like (LOC101863543), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101863543 ( XM_013088375.1 ) cDNA ORF clone, Aplysia californica(California sea hare) -> XP_012943829.1 Aplysia californica fibroin heavy chain-like (LOC101863543), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAp160532
Clone ID Related Accession (Same CDS sequence)	XM_013088375.1
Accession Version	XM_013088375.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-06
Organism	Aplysia californica(California sea hare)
Product	ribonucleoprotein PTB-binding 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004798001.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aplysia californica Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGA CCCGGAGTGG TGGTGCGCTG AAGCGCAGAG CGAGGGAAGA ATTCAACAAA 
CATTGCATCC TTGAGCTGTC CAATGTTCCT CCAAGTACAG ATGGGGAAAT AAAAACTTTC 
ATGGCAGGAA TCAAAGTGTC AAAAATCAAA AGAAATGAAG AAACGGCTCT TGTCACCTTG 
GTCCAAGGGG AACAGTTAGA ACAAGCCGTT GAGAAGTTGA ATGAAAAGCA GATGAAGGAC 
AACACTGTGG CTGTGAAACC AGGATGCAGT GGTAAAACCA TTTGCGTGGC TCATATCCCC 
CTGACCTACA CTGAAGACAT GTTCCGTGTC TTGTGTGAGA AATATGGAAC TGTGGAATTT 
TCTTATATCA TGCGGTCTGA AGAGACAGGT GCTAGTAAAG GTTATGGTAT TGTTGAGTTT 
AAAGAGGAGG TTGAGCAAAG TAAAGTCATC AAATCTGAGC TAGACTGGCA CACAGTGGAT 
GGTCAGTCTC TGCATGCTGA CATTCTGGAC GAGTCCTGTC TAGCTAACTT TGAACAGCTG 
CAGTCTCGCT GCCTGCTCAT CAACGACATG CCAGAAGACT TTGTTGATGT TTCCAAATTG 
CGTGAGATGT ATGGATGCGT GACTAGTCCA GTGTATTGTC AGATTATTGC TAAAGGAGAG 
AAGTCTCTTG GATACGGCAT TATCGAATTC CGCACCCCGG AGGATGCTGA GCAGACATGG 
CTGAAAATGC ACACGGAAAA AATAGAAGAT AAAGAAGTTG TAGCGACTTT CTGTATCCCA 
GGGAAGTCAG CTGTTGTGAT CAACAATAGG ATAATGTGGA AATATGGAGA CAAACTTCAG 
AAGAGCAGTA GCCTTCTTCC TGATCCCGTT GCTGCAAAGC CTGTCATTGC TGGGAATCCA 
ATTGTCATTA GTTTGTCCAA CATGAACTCT AAATTAATGG ATGAGTTCAC AAAAGTGCTT 
GGTGAATTGC AGCAAGCATA TGTGTCTCAG ATGATGTCAC CTGTACACAA ACCAGGTTTG 
TTGGGACCGG CGCCATCACT TCCTCTAAGC CCCATGATGA ACCCAAACAT GCAGTTGGGG 
CTTTTGGTGA TGCTTGCTAT CCACATTCAG GGTGCAAAGA AAGAACAGTT TAAAGGAGTC 
TTGGCCAAGC AGCTGAACAA CTTGAGTGAA CCGGAAGGCG TAGATAGTGG ACGTAAGCCA 
TCCATTTTGG GGGATCCCTT GACTGGACAA GCCAACGTGA TTCTTACCAC TCTGAAGCAA 
CAGCTGAACC AGGTCGCTCT CACTGGAGAA GATGGAGAGA CTGTTGAAGA ACCAGAGGCT 
GTTGATGACA AAATCCAGCA CATTGTCAAG AAGTTTATTG CCAATGGACG CTACCTCAAC 
CTGAGCCTCC TCACCAACCT GGGTCAGCTG CTGGTCACCT TGAACAAAGT GGCCTCCGAC 
ACTCCCAGAA GTGGTCCCAC ATCTTTGGGA GGTGGTAGGG GCCAGTCTCT ATTGGGTCCC 
GTACCTGGAT CTGGTCCACC CCAGCCTCTA ATGGGGCCCC CTATTGGCCA AAACCTCCTG 
TACCAGATGT CGATGGGAGG TGGGGGACCT GGTGGTCCCG GCGGCGGTGG CCCAGGAGGC 
AGAGGACTGC TGGGTGATGT GCCCAAGGAC GACCCCGGCA TGGGCAAGCC AATGGGCAAC 
CACATGCGAA ACCAGATGGG CAACCAGCGA ATGAAGAGCG GAGGGGGTGG ATTTGGGGGT 
GGCCCCGGCG GCAGTGGATT TGGAGGCGGA CCTGGTGGAC CTGGGAGTGG ATTTGGATCT 
GGAGGTGGCG GATTTGGAAG CGGAGGAGGA GGAGGTGGGT TTGGAGGCGG ACCTGGCAGT 
GGATTTGGCG GAGGCGCTGG CAGTGGTTTT GGCGGAGGAC CCGGTAGTGG ATTTGGTGGA 
GGTGCTGGAG GAGGTGGATT TGGCGGGGGT TCTGGAGGTA GCGGTTTTGG TGCTGGTTCC 
GGAGGTGGTT TTGGCGGAAG TGGTGGCGGA GGAGGAGGTG GAGGTGGATC GGGTGGAGGT 
GGAAAGTCGC TGTTGGGCAA GCCTCCCAGT TTCAAGTCGA ACCAGGGTGG AAATACTGGC 
AACGCCGCAT TGGACCAAGG ATCCTGGAAG AGTATGGAAT CTGGGCGTTG GGGTTCTGGA 
AACAACTATG AGAGCGGCTA CGGCAACAAA ACCAATATGT CTGGAGGATA CAATGACTAC 
TCGTATGGAA ATGATAATTA CTCGCAGGAC ATGAGTAGCG GAGGCTATGG TGGTTATGGT 
GATGGCAACT CGGACTACTC CTATTATCAA CAGTTTGGAT ATGGTCAGGA TAACTCGTAC 
CAAGGGGACA GTGGGTATAA TCAGTTTGGT GGCTCTGGCT TTGGCTCTGG AGGTGGCATG 
GGCTCTGGAG GTGGCATGAG CGGCAGCGGC TCTGGCAATA TGGGCGGTGG TGGCAGCGGC 
ACTGGTGGAT ATGGAATGGG TGGGGGCCTT GGAGGCAACC AAGCATCAGG ATGGACAAGC 
TTTCGGCAAA GGAGGCAACA GTGGCCTTGG CCAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012943829.1
CDS	26..2584
Translation	MASTRSGGALKRRAREEFNKHCILELSNVPPSTDGEIKTFMAGIKVSKIKRNEETALVTLVQGEQLEQAVEKLNEKQMKDNTVAVKPGCSGKTICVAHIPLTYTEDMFRVLCEKYGTVEFSYIMRSEETGASKGYGIVEFKEEVEQSKVIKSELDWHTVDGQSLHADILDESCLANFEQLQSRCLLINDMPEDFVDVSKLREMYGCVTSPVYCQIIAKGEKSLGYGIIEFRTPEDAEQTWLKMHTEKIEDKEVVATFCIPGKSAVVINNRIMWKYGDKLQKSSSLLPDPVAAKPVIAGNPIVISLSNMNSKLMDEFTKVLGELQQAYVSQMMSPVHKPGLLGPAPSLPLSPMMNPNMQLGLLVMLAIHIQGAKKEQFKGVLAKQLNNLSEPEGVDSGRKPSILGDPLTGQANVILTTLKQQLNQVALTGEDGETVEEPEAVDDKIQHIVKKFIANGRYLNLSLLTNLGQLLVTLNKVASDTPRSGPTSLGGGRGQSLLGPVPGSGPPQPLMGPPIGQNLLYQMSMGGGGPGGPGGGGPGGRGLLGDVPKDDPGMGKPMGNHMRNQMGNQRMKSGGGGFGGGPGGSGFGGGPGGPGSGFGSGGGGFGSGGGGGGFGGGPGSGFGGGAGSGFGGGPGSGFGGGAGGGGFGGGSGGSGFGAGSGGGFGGSGGGGGGGGGSGGGGKSLLGKPPSFKSNQGGNTGNAALDQGSWKSMESGRWGSGNNYESGYGNKTNMSGGYNDYSYGNDNYSQDMSSGGYGGYGDGNSDYSYYQQFGYGQDNSYQGDSGYNQFGGSGFGSGGGMGSGGGMSGSGSGNMGGGGSGTGGYGMGGGLGGNQASGWTSFRQRRQQWPWPR

Target ORF information:

RefSeq Version	XM_013088375.1
Organism	Aplysia californica(California sea hare)
Definition	Aplysia californica fibroin heavy chain-like (LOC101863543), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013088375.1

ORF Insert Sequence:

ATGGCGTCGA CCCGGAGTGG TGGTGCGCTG AAGCGCAGAG CGAGGGAAGA ATTCAACAAA 
CATTGCATCC TTGAGCTGTC CAATGTTCCT CCAAGTACAG ATGGGGAAAT AAAAACTTTC 
ATGGCAGGAA TCAAAGTGTC AAAAATCAAA AGAAATGAAG AAACGGCTCT TGTCACCTTG 
GTCCAAGGGG AACAGTTAGA ACAAGCCGTT GAGAAGTTGA ATGAAAAGCA GATGAAGGAC 
AACACTGTGG CTGTGAAACC AGGATGCAGT GGTAAAACCA TTTGCGTGGC TCATATCCCC 
CTGACCTACA CTGAAGACAT GTTCCGTGTC TTGTGTGAGA AATATGGAAC TGTGGAATTT 
TCTTATATCA TGCGGTCTGA AGAGACAGGT GCTAGTAAAG GTTATGGTAT TGTTGAGTTT 
AAAGAGGAGG TTGAGCAAAG TAAAGTCATC AAATCTGAGC TAGACTGGCA CACAGTGGAT 
GGTCAGTCTC TGCATGCTGA CATTCTGGAC GAGTCCTGTC TAGCTAACTT TGAACAGCTG 
CAGTCTCGCT GCCTGCTCAT CAACGACATG CCAGAAGACT TTGTTGATGT TTCCAAATTG 
CGTGAGATGT ATGGATGCGT GACTAGTCCA GTGTATTGTC AGATTATTGC TAAAGGAGAG 
AAGTCTCTTG GATACGGCAT TATCGAATTC CGCACCCCGG AGGATGCTGA GCAGACATGG 
CTGAAAATGC ACACGGAAAA AATAGAAGAT AAAGAAGTTG TAGCGACTTT CTGTATCCCA 
GGGAAGTCAG CTGTTGTGAT CAACAATAGG ATAATGTGGA AATATGGAGA CAAACTTCAG 
AAGAGCAGTA GCCTTCTTCC TGATCCCGTT GCTGCAAAGC CTGTCATTGC TGGGAATCCA 
ATTGTCATTA GTTTGTCCAA CATGAACTCT AAATTAATGG ATGAGTTCAC AAAAGTGCTT 
GGTGAATTGC AGCAAGCATA TGTGTCTCAG ATGATGTCAC CTGTACACAA ACCAGGTTTG 
TTGGGACCGG CGCCATCACT TCCTCTAAGC CCCATGATGA ACCCAAACAT GCAGTTGGGG 
CTTTTGGTGA TGCTTGCTAT CCACATTCAG GGTGCAAAGA AAGAACAGTT TAAAGGAGTC 
TTGGCCAAGC AGCTGAACAA CTTGAGTGAA CCGGAAGGCG TAGATAGTGG ACGTAAGCCA 
TCCATTTTGG GGGATCCCTT GACTGGACAA GCCAACGTGA TTCTTACCAC TCTGAAGCAA 
CAGCTGAACC AGGTCGCTCT CACTGGAGAA GATGGAGAGA CTGTTGAAGA ACCAGAGGCT 
GTTGATGACA AAATCCAGCA CATTGTCAAG AAGTTTATTG CCAATGGACG CTACCTCAAC 
CTGAGCCTCC TCACCAACCT GGGTCAGCTG CTGGTCACCT TGAACAAAGT GGCCTCCGAC 
ACTCCCAGAA GTGGTCCCAC ATCTTTGGGA GGTGGTAGGG GCCAGTCTCT ATTGGGTCCC 
GTACCTGGAT CTGGTCCACC CCAGCCTCTA ATGGGGCCCC CTATTGGCCA AAACCTCCTG 
TACCAGATGT CGATGGGAGG TGGGGGACCT GGTGGTCCCG GCGGCGGTGG CCCAGGAGGC 
AGAGGACTGC TGGGTGATGT GCCCAAGGAC GACCCCGGCA TGGGCAAGCC AATGGGCAAC 
CACATGCGAA ACCAGATGGG CAACCAGCGA ATGAAGAGCG GAGGGGGTGG ATTTGGGGGT 
GGCCCCGGCG GCAGTGGATT TGGAGGCGGA CCTGGTGGAC CTGGGAGTGG ATTTGGATCT 
GGAGGTGGCG GATTTGGAAG CGGAGGAGGA GGAGGTGGGT TTGGAGGCGG ACCTGGCAGT 
GGATTTGGCG GAGGCGCTGG CAGTGGTTTT GGCGGAGGAC CCGGTAGTGG ATTTGGTGGA 
GGTGCTGGAG GAGGTGGATT TGGCGGGGGT TCTGGAGGTA GCGGTTTTGG TGCTGGTTCC 
GGAGGTGGTT TTGGCGGAAG TGGTGGCGGA GGAGGAGGTG GAGGTGGATC GGGTGGAGGT 
GGAAAGTCGC TGTTGGGCAA GCCTCCCAGT TTCAAGTCGA ACCAGGGTGG AAATACTGGC 
AACGCCGCAT TGGACCAAGG ATCCTGGAAG AGTATGGAAT CTGGGCGTTG GGGTTCTGGA 
AACAACTATG AGAGCGGCTA CGGCAACAAA ACCAATATGT CTGGAGGATA CAATGACTAC 
TCGTATGGAA ATGATAATTA CTCGCAGGAC ATGAGTAGCG GAGGCTATGG TGGTTATGGT 
GATGGCAACT CGGACTACTC CTATTATCAA CAGTTTGGAT ATGGTCAGGA TAACTCGTAC 
CAAGGGGACA GTGGGTATAA TCAGTTTGGT GGCTCTGGCT TTGGCTCTGG AGGTGGCATG 
GGCTCTGGAG GTGGCATGAG CGGCAGCGGC TCTGGCAATA TGGGCGGTGG TGGCAGCGGC 
ACTGGTGGAT ATGGAATGGG TGGGGGCCTT GGAGGCAACC AAGCATCAGG ATGGACAAGC 
TTTCGGCAAA GGAGGCAACA GTGGCCTTGG CCAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101863543 ( XM_013088374.1 ) cDNA ORF clone, Aplysia californica(California sea hare) -> XP_012943828.1 Aplysia californica fibroin heavy chain-like (LOC101863543), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAp160531
Clone ID Related Accession (Same CDS sequence)	XM_013088374.1
Accession Version	XM_013088374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-06
Organism	Aplysia californica(California sea hare)
Product	fibroin heavy chain-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004798001.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aplysia californica Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGA CCCGGAGTGG TGGTGCGCTG AAGCGCAGAG CGAGGGAAGA ATTCAACAAA 
CATTGCATCC TTGAGCTGTC CAATGTTCCT CCAAGTACAG ATGGGGAAAT AAAAACTTTC 
ATGGCAGGAA TCAAAGTGTC AAAAATCAAA AGAAATGAAG AAACGGCTCT TGTCACCTTG 
GTCCAAGGGG AACAGTTAGA ACAAGCCGTT GAGAAGTTGA ATGAAAAGCA GATGAAGGAC 
AACACTGTGG CTGTGAAACC AGGATGCAGT GGTAAAACCA TTTGCGTGGC TCATATCCCC 
CTGACCTACA CTGAAGACAT GTTCCGTGTC TTGTGTGAGA AATATGGAAC TGTGGAATTT 
TCTTATATCA TGCGGTCTGA AGAGACAGGT GCTAGTAAAG GTTATGGTAT TGTTGAGTTT 
AAAGAGGAGG TTGAGCAAAG TAAAGTCATC AAATCTGAGC TAGACTGGCA CACAGTGGAT 
GGTCAGTCTC TGCATGCTGA CATTCTGGAC GAGTCCTGTC TAGCTAACTT TGAACAGCTG 
CAGTCTCGCT GCCTGCTCAT CAACGACATG CCAGAAGACT TTGTTGATGT TTCCAAATTG 
CGTGAGATGT ATGGATGCGT GACTAGTCCA GTGTATTGTC AGATTATTGC TAAAGGAGAG 
AAGTCTCTTG GATACGGCAT TATCGAATTC CGCACCCCGG AGGATGCTGA GCAGACATGG 
CTGAAAATGC ACACGGAAAA AATAGAAGAT AAAGAAGTTG TAGCGACTTT CTGTATCCCA 
GGGAAGTCAG CTGTTGTGAT CAACAATAGG ATAATGTGGA AATATGGAGA CAAACTTCAG 
AAGAGCAGTA GCCTTCTTCC TGATCCCGTT GCTGCAAAGC CTGTCATTGC TGGGAATCCA 
ATTGTCATTA GTTTGTCCAA CATGAACTCT AAATTAATGG ATGAGTTCAC AAAAGTGCTT 
GGTGAATTGC AGCAAGCATA TGTGTCTCAG ATGATGTCAC CTGTACACAA ACCAGGTTTG 
TTGGGACCGG CGCCATCACT TCCTCTAAGC CCCATGATGA ACCCAAACAT GCAGTTGGGG 
CTTTTGGTGA TGCTTGCTAT CCACATTCAG GGTGCAAAGA AAGAACAGTT TAAAGGAGTC 
TTGGCCAAGC AGCTGAACAA CTTGAGTGAA CCGGAAGGCG TAGATAGTGG ACGTAAGCCA 
TCCATTTTGG GGGATCCCTT GACTGGACAA GCCAACGTGA TTCTTACCAC TCTGAAGCAA 
CAGCTGAACC AGGTCGCTCT CACTGGAGAA GATGGAGAGA CTGTTGAAGA ACCAGAGGCT 
GTTGATGACA AAATCCAGCA CATTGTCAAG AAGTTTATTG CCAATGGACG CTACCTCAAC 
CTGAGCCTCC TCACCAACCT GGGTCAGCTG CTGGTCACCT TGAACAAAGT GGCCTCCGAC 
ACTCCCAGAA GTGGTCCCAC ATCTTTGGGA GGTGGTAGGG GCCAGTCTCT ATTGGGTCCC 
GTACCTGGAT CTGGTCCACC CCAGCCTCTA ATGGGGCCCC CTATTGGCCA AAACCTCCTG 
TACCAGATGT CGATGGGAGG TGGGGGACCT GGTGGTCCCG GCGGCGGTGG CCCAGGAGGC 
AGAGGACTGC TGGGTGATGT GCCCAAGGAC GACCCCGGCA TGGGCAAGCC AATGGGCAAC 
CACATGCGAA ACCAGATGGG CAACCAGCGA ATGAAGAGCG GAGGGGGTGG ATTTGGGGGT 
GGCCCCGGCG GCAGTGGATT TGGAGGCGGA CCTGGTGGAC CTGGGAGTGG ATTTGGATCT 
GGAGGTGGCG GATTTGGAAG CGGAGGAGGA GGAGGTGGGT TTGGAGGCGG ACCTGGCAGT 
GGATTTGGCG GAGGCGCTGG CAGTGGTTTT GGCGGAGGAC CCGGTAGTGG ATTTGGTGGA 
GGTGCTGGAG GAGGTGGATT TGGCGGGGGT TCTGGAGGTA GCGGTTTTGG TGCTGGTTCC 
GGAGGTGGTT TTGGCGGAAG TGGTGGCGGA GGAGGAGGTG GAGGTGGATC GGGTGGAGGT 
GGAAAGTCGC TGTTGGGCAA GCCTCCCAGT TTCAAGTCGA ACCAGGGTGG AAATACTGGC 
AACGCCGCAT TGGACCAAGG ATCCTGGAAG AGTATGGAAT CTGGGCGTTG GGGTTCTGGA 
AACAACTATG AGAGCGGCTA CGGCAACAAA ACCAATATGT CTGGAGGATA CAATGACTAC 
TCGTATGGAA ATGATAATTA CTCGCAGGAC ATGAGTAGCG GAGGCTATGG TGGTTATGGT 
GATGGCAACT CGGACTACTC CTATTATCAA CAGTTTGGAT ATGGTCAGGA TAACTCGTAC 
CAAGGGGACA GTGGGTATAA TCAGTTTGGT GGCTCTGGCT TTGGCTCTGG AGGTGGCATG 
GGCTCTGGAG GTGGCATGAG CGGCAGCGGC TCTGGCAATA TGGGCGGTGG TGGCAGCGGC 
ACTGGTGGAT ATGGAATGGG TGGGGGCCTT GGAGGCAACC AAGCATCAGG TGTGAGCTTC 
CAGGATGGAC AAGCTTTCGG CAAAGGAGGC AACAGTGGCC TTGGCCAAGA TAATAGCAAC 
TATGGTGGGG ATGGGTACGG GGGAGGAATG GGAAACAATA ATTATGGCTA CGGCGGAAGA 
GATGATTACA ACACTGGGAC TTACGATTCC TACGGGTCTG TTGGTTTGGG TGGGAACTAC 
TCTAGCGGAA TGGGAGATGG CATGGGAGGA ATGGGCGGTG CCAGTGGCGG CGGGGGTAGC 
ATGAGTGGGA TGGGAGGGAG CGGAATGAGT GGGATGGGCA GCGGCTCGAT GAGTGGGATG 
GGCAGCATGA GCGGTATGGG CAGCGGCTCC ATGAGTGGGA TGGGAGGAAT GGGCAACACC 
AACTCGATGA GTGGGATGGG CAGTTCGTCC ATGAGCGGAA TGGGAAGCAC CTCTATGAGT 
GGGATGGGAG GAGGCTCTAT GAGTGGGATG GGAGGCGGCT CTATGAGTGG AATGGGCTCT 
GGTATGAGCG GGATGGGCGG AGGCTCTATG AGCGGGATGG GTGGAACTTC CATGGGGGGA 
ATGGGCGGGA TGAGCAGCAG CATGAATAAC GGCATGGGTG GCATGAGCGA CATGTCGTCC 
AGTGCCTACT CCACTGGTAT GAGCGCGTCG GGCAGCAAGG GCTACGGTAG CTCCGGTACC 
GGCATGATGG GAGGCAGCAG CAGCTATGGG AGCAGTGGTG GCATGGGCAC GGGAGGCTCC 
ATGGGCAACA AGATGGGCGG AGGGCTGGGT GGCAGAGACC AAAGTATGAT GGGAAGTTCC 
TACTACCAGG GCGGTGCAGG GAACAGCGTG TCAAACTCTG GACTGTTGCC ATCACCTTCA 
AAATATATGA CCCCACCACA AAAACGCAGC CACAACCAAC TCTTGCCCAA GCCAGAGCCT 
AGCCCTGATG GTGCTGATTA TATTGGTCAA CACTCTCAAG GCATCGGAGG ACACTACCAG 
GACAGCTTCA AGCGCCCACG TCTCTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012943828.1
CDS	24..3533
Translation	MASTRSGGALKRRAREEFNKHCILELSNVPPSTDGEIKTFMAGIKVSKIKRNEETALVTLVQGEQLEQAVEKLNEKQMKDNTVAVKPGCSGKTICVAHIPLTYTEDMFRVLCEKYGTVEFSYIMRSEETGASKGYGIVEFKEEVEQSKVIKSELDWHTVDGQSLHADILDESCLANFEQLQSRCLLINDMPEDFVDVSKLREMYGCVTSPVYCQIIAKGEKSLGYGIIEFRTPEDAEQTWLKMHTEKIEDKEVVATFCIPGKSAVVINNRIMWKYGDKLQKSSSLLPDPVAAKPVIAGNPIVISLSNMNSKLMDEFTKVLGELQQAYVSQMMSPVHKPGLLGPAPSLPLSPMMNPNMQLGLLVMLAIHIQGAKKEQFKGVLAKQLNNLSEPEGVDSGRKPSILGDPLTGQANVILTTLKQQLNQVALTGEDGETVEEPEAVDDKIQHIVKKFIANGRYLNLSLLTNLGQLLVTLNKVASDTPRSGPTSLGGGRGQSLLGPVPGSGPPQPLMGPPIGQNLLYQMSMGGGGPGGPGGGGPGGRGLLGDVPKDDPGMGKPMGNHMRNQMGNQRMKSGGGGFGGGPGGSGFGGGPGGPGSGFGSGGGGFGSGGGGGGFGGGPGSGFGGGAGSGFGGGPGSGFGGGAGGGGFGGGSGGSGFGAGSGGGFGGSGGGGGGGGGSGGGGKSLLGKPPSFKSNQGGNTGNAALDQGSWKSMESGRWGSGNNYESGYGNKTNMSGGYNDYSYGNDNYSQDMSSGGYGGYGDGNSDYSYYQQFGYGQDNSYQGDSGYNQFGGSGFGSGGGMGSGGGMSGSGSGNMGGGGSGTGGYGMGGGLGGNQASGVSFQDGQAFGKGGNSGLGQDNSNYGGDGYGGGMGNNNYGYGGRDDYNTGTYDSYGSVGLGGNYSSGMGDGMGGMGGASGGGGSMSGMGGSGMSGMGSGSMSGMGSMSGMGSGSMSGMGGMGNTNSMSGMGSSSMSGMGSTSMSGMGGGSMSGMGGGSMSGMGSGMSGMGGGSMSGMGGTSMGGMGGMSSSMNNGMGGMSDMSSSAYSTGMSASGSKGYGSSGTGMMGGSSSYGSSGGMGTGGSMGNKMGGGLGGRDQSMMGSSYYQGGAGNSVSNSGLLPSPSKYMTPPQKRSHNQLLPKPEPSPDGADYIGQHSQGIGGHYQDSFKRPRLY

Target ORF information:

RefSeq Version	XM_013088374.1
Organism	Aplysia californica(California sea hare)
Definition	Aplysia californica fibroin heavy chain-like (LOC101863543), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013088374.1

ORF Insert Sequence:

ATGGCGTCGA CCCGGAGTGG TGGTGCGCTG AAGCGCAGAG CGAGGGAAGA ATTCAACAAA 
CATTGCATCC TTGAGCTGTC CAATGTTCCT CCAAGTACAG ATGGGGAAAT AAAAACTTTC 
ATGGCAGGAA TCAAAGTGTC AAAAATCAAA AGAAATGAAG AAACGGCTCT TGTCACCTTG 
GTCCAAGGGG AACAGTTAGA ACAAGCCGTT GAGAAGTTGA ATGAAAAGCA GATGAAGGAC 
AACACTGTGG CTGTGAAACC AGGATGCAGT GGTAAAACCA TTTGCGTGGC TCATATCCCC 
CTGACCTACA CTGAAGACAT GTTCCGTGTC TTGTGTGAGA AATATGGAAC TGTGGAATTT 
TCTTATATCA TGCGGTCTGA AGAGACAGGT GCTAGTAAAG GTTATGGTAT TGTTGAGTTT 
AAAGAGGAGG TTGAGCAAAG TAAAGTCATC AAATCTGAGC TAGACTGGCA CACAGTGGAT 
GGTCAGTCTC TGCATGCTGA CATTCTGGAC GAGTCCTGTC TAGCTAACTT TGAACAGCTG 
CAGTCTCGCT GCCTGCTCAT CAACGACATG CCAGAAGACT TTGTTGATGT TTCCAAATTG 
CGTGAGATGT ATGGATGCGT GACTAGTCCA GTGTATTGTC AGATTATTGC TAAAGGAGAG 
AAGTCTCTTG GATACGGCAT TATCGAATTC CGCACCCCGG AGGATGCTGA GCAGACATGG 
CTGAAAATGC ACACGGAAAA AATAGAAGAT AAAGAAGTTG TAGCGACTTT CTGTATCCCA 
GGGAAGTCAG CTGTTGTGAT CAACAATAGG ATAATGTGGA AATATGGAGA CAAACTTCAG 
AAGAGCAGTA GCCTTCTTCC TGATCCCGTT GCTGCAAAGC CTGTCATTGC TGGGAATCCA 
ATTGTCATTA GTTTGTCCAA CATGAACTCT AAATTAATGG ATGAGTTCAC AAAAGTGCTT 
GGTGAATTGC AGCAAGCATA TGTGTCTCAG ATGATGTCAC CTGTACACAA ACCAGGTTTG 
TTGGGACCGG CGCCATCACT TCCTCTAAGC CCCATGATGA ACCCAAACAT GCAGTTGGGG 
CTTTTGGTGA TGCTTGCTAT CCACATTCAG GGTGCAAAGA AAGAACAGTT TAAAGGAGTC 
TTGGCCAAGC AGCTGAACAA CTTGAGTGAA CCGGAAGGCG TAGATAGTGG ACGTAAGCCA 
TCCATTTTGG GGGATCCCTT GACTGGACAA GCCAACGTGA TTCTTACCAC TCTGAAGCAA 
CAGCTGAACC AGGTCGCTCT CACTGGAGAA GATGGAGAGA CTGTTGAAGA ACCAGAGGCT 
GTTGATGACA AAATCCAGCA CATTGTCAAG AAGTTTATTG CCAATGGACG CTACCTCAAC 
CTGAGCCTCC TCACCAACCT GGGTCAGCTG CTGGTCACCT TGAACAAAGT GGCCTCCGAC 
ACTCCCAGAA GTGGTCCCAC ATCTTTGGGA GGTGGTAGGG GCCAGTCTCT ATTGGGTCCC 
GTACCTGGAT CTGGTCCACC CCAGCCTCTA ATGGGGCCCC CTATTGGCCA AAACCTCCTG 
TACCAGATGT CGATGGGAGG TGGGGGACCT GGTGGTCCCG GCGGCGGTGG CCCAGGAGGC 
AGAGGACTGC TGGGTGATGT GCCCAAGGAC GACCCCGGCA TGGGCAAGCC AATGGGCAAC 
CACATGCGAA ACCAGATGGG CAACCAGCGA ATGAAGAGCG GAGGGGGTGG ATTTGGGGGT 
GGCCCCGGCG GCAGTGGATT TGGAGGCGGA CCTGGTGGAC CTGGGAGTGG ATTTGGATCT 
GGAGGTGGCG GATTTGGAAG CGGAGGAGGA GGAGGTGGGT TTGGAGGCGG ACCTGGCAGT 
GGATTTGGCG GAGGCGCTGG CAGTGGTTTT GGCGGAGGAC CCGGTAGTGG ATTTGGTGGA 
GGTGCTGGAG GAGGTGGATT TGGCGGGGGT TCTGGAGGTA GCGGTTTTGG TGCTGGTTCC 
GGAGGTGGTT TTGGCGGAAG TGGTGGCGGA GGAGGAGGTG GAGGTGGATC GGGTGGAGGT 
GGAAAGTCGC TGTTGGGCAA GCCTCCCAGT TTCAAGTCGA ACCAGGGTGG AAATACTGGC 
AACGCCGCAT TGGACCAAGG ATCCTGGAAG AGTATGGAAT CTGGGCGTTG GGGTTCTGGA 
AACAACTATG AGAGCGGCTA CGGCAACAAA ACCAATATGT CTGGAGGATA CAATGACTAC 
TCGTATGGAA ATGATAATTA CTCGCAGGAC ATGAGTAGCG GAGGCTATGG TGGTTATGGT 
GATGGCAACT CGGACTACTC CTATTATCAA CAGTTTGGAT ATGGTCAGGA TAACTCGTAC 
CAAGGGGACA GTGGGTATAA TCAGTTTGGT GGCTCTGGCT TTGGCTCTGG AGGTGGCATG 
GGCTCTGGAG GTGGCATGAG CGGCAGCGGC TCTGGCAATA TGGGCGGTGG TGGCAGCGGC 
ACTGGTGGAT ATGGAATGGG TGGGGGCCTT GGAGGCAACC AAGCATCAGG TGTGAGCTTC 
CAGGATGGAC AAGCTTTCGG CAAAGGAGGC AACAGTGGCC TTGGCCAAGA TAATAGCAAC 
TATGGTGGGG ATGGGTACGG GGGAGGAATG GGAAACAATA ATTATGGCTA CGGCGGAAGA 
GATGATTACA ACACTGGGAC TTACGATTCC TACGGGTCTG TTGGTTTGGG TGGGAACTAC 
TCTAGCGGAA TGGGAGATGG CATGGGAGGA ATGGGCGGTG CCAGTGGCGG CGGGGGTAGC 
ATGAGTGGGA TGGGAGGGAG CGGAATGAGT GGGATGGGCA GCGGCTCGAT GAGTGGGATG 
GGCAGCATGA GCGGTATGGG CAGCGGCTCC ATGAGTGGGA TGGGAGGAAT GGGCAACACC 
AACTCGATGA GTGGGATGGG CAGTTCGTCC ATGAGCGGAA TGGGAAGCAC CTCTATGAGT 
GGGATGGGAG GAGGCTCTAT GAGTGGGATG GGAGGCGGCT CTATGAGTGG AATGGGCTCT 
GGTATGAGCG GGATGGGCGG AGGCTCTATG AGCGGGATGG GTGGAACTTC CATGGGGGGA 
ATGGGCGGGA TGAGCAGCAG CATGAATAAC GGCATGGGTG GCATGAGCGA CATGTCGTCC 
AGTGCCTACT CCACTGGTAT GAGCGCGTCG GGCAGCAAGG GCTACGGTAG CTCCGGTACC 
GGCATGATGG GAGGCAGCAG CAGCTATGGG AGCAGTGGTG GCATGGGCAC GGGAGGCTCC 
ATGGGCAACA AGATGGGCGG AGGGCTGGGT GGCAGAGACC AAAGTATGAT GGGAAGTTCC 
TACTACCAGG GCGGTGCAGG GAACAGCGTG TCAAACTCTG GACTGTTGCC ATCACCTTCA 
AAATATATGA CCCCACCACA AAAACGCAGC CACAACCAAC TCTTGCCCAA GCCAGAGCCT 
AGCCCTGATG GTGCTGATTA TATTGGTCAA CACTCTCAAG GCATCGGAGG ACACTACCAG 
GACAGCTTCA AGCGCCCACG TCTCTACTAA