AgaP_AGAP003979 cDNA ORF clone, Anopheles gambiae str. PEST

The following AgaP_AGAP003979 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AgaP_AGAP003979 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OAh06167 XM_318429.4
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Anopheles gambiae str. PEST AGAP003979-RA (AgaP_AGAP003979), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OAh06167
    Clone ID Related Accession (Same CDS sequence) XM_318429.4
    Accession Version XM_318429.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3981bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-25
    Organism Anopheles gambiae str. PEST
    Product AGAP003979-PA
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_078266). On Oct 15, 2007 this sequence version replaced XM_318429.3. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGGAACC TGTACCGTGT GTCACACTCT CGCTTCGACC TATCGGAAAA TGCGCTCCGT 
    TCAGTTCGGT CCGGTTTTGC GATCGATCAA AACACGGACC ACAAGTTTTA CTTCGTGCAG
    GACAGTGGTG TGTACTGCTG TCCGCTGTTC GTTGACCGGG AGCATCCGTT CTGCGAACCG
    CTGCTGTCGC AATCGGACGG ATACGAGAAC ATCATAGCGC TGGAACATCT GGCCCTGTCC
    GACGAGCTTT GCTGTGCGTC GTCGAGTGGA AACGTGTGGT CCTGCAAGCT GGAGACGGGA
    GTGGTGGAAG AAGTCACGCA CTGCAAGCAC GGCATCAGGG CGATGCAGTG GAGCCCGGAC
    CAGGAGCTGG TCGTGTTTGT GGACGGCGAG CTGAACGTTG TCACCATGAT CGGGAGCGAG
    TTCGAGCCGA TCAACGAGGT ACAGCTGAAG GACGACACGT TCGGCGACCG GCAGTTCATG
    AGTGTGGGCT GGGGCAAGAA GGAAACTCAA TTTCACGGAT CGGAGGGAAA GGCTGCCGCA
    AAGAAGGGTA CCACGCCCAG CGAGCCGAGC ACAGCGGAGA CGGAGCGACA GCTGGACGCG
    ACCGTAAACA TCAGCTGGCG TGCGGATGGA GAATACTTTG CGGTGGGCTA CCTGGCACCG
    TTTGGTCAGC GAGCTTTCAA AGTGTTCAAC AAGGAGGGCG CATTGCAATA CACGTCGGAA
    AAGTGTCACG GGCTGGAGGG TTCGCTTGCC TGGCGTCCGT CGGGCAATTG GATTGCGATC
    CCGCAGGAAC TTAAAGATCG GTATGTTGTA GCGTTGTTCG AGAAGAATGG GCTGCGACAC
    CGGGAGATTC CGCTGACGTT GAACCCTCGC GCCGGTCAAA GCGTACAGTC GCTTCACTGG
    AGTGCGGACT CGGACGTGCT CGCCGTGCAT TGGGTGGATG GGGAGCAAGG GACTACCGGC
    GTGTTGCTGT ACGTGATCGG AAACTATCAT TGGTACTTGA AGCAAAGCTT GACCTACCCG
    CCTGGTACCG CGCTAACTGG ACTGCAGTGG GATCAGCGTC ACACGGCCGG CAAAACCCTC
    CATTTGTTTT CATCTGACCC AGGCGTTTAT GAGTGCATCC GGTTCGATTT TCGAGTGGAT
    CGTTCCGTGG GCCTTTCGGA AGAAGATCAA ACGATGGTAG CGGTGATCGA TGGAAAGCGA
    CTGCTGTTGA CCGGTTTCCG GCAGGCTGTC ATACCGCCGC CCATGTGTGG CTTTACGCTG
    GAGCAAGAGC ATCCCATCAA TGCGACCGGG TTCATTCGTA CAGCCGGACG CTGCGAAGCA
    TTAGGGCAGG ACATAAGCTC AAACGCCTTC TTTACCGTGG ATTGCCGTGG TGAAATCATT
    TTGTACGATG CTGTATTTAC CAAGACAGCG GGTAGATCCA TATTATCTGG AGTTACCGTA
    TTGCTGAAGA TTTCACCAGA ACAGCTGAAA ATGTTCAACG AAGATTTTCC CCAACAGCAC
    CACTGCGGAC TTCACTACCT TTGGTTGAAC GCGGATAATT TCGTGATGGA TCACAGTCAA
    CTACGTTGCG GCGTTTATAC CATTGCAATG CACAATGACG TCCCCGGCTT GAAGAAAGTA
    TGGCACATGG AGATGGAAAA CAGGGAGGAA ATCGGTTGCA TTGAATCGTC CGGAGCGGAT
    AGTATTCTTG TTGAGCTGCT ATCTGGCCAC GTAATGGAGG TAAAAGGATT GACCGGCCAG
    CAGGATGGTG CATTGGAAAC AAGCTCACGC AATCACAGCG TTTTGCCGGT ATTTTGCGAA
    CAACTGTTTG TCGATCGCAA CCGTCCCGAG CGGACAGTGT ACGCGCTGGC GCAGAACCGC
    CGTCACCTGT ACCGTGATGG AAGTCTTCTC GCGTCGGACA TAACGTCTGC TCTGCTTACC
    GACAGCTACC TACTTTGCAC CACTATTTCG GAGCTTAAAT TCATCGCACT CGGTGCAGCG
    GTTGTGGCAC CCGGCCGTGA TCCGATCATC GGCGAGCGAA GGGTCGAACG TGGCTCGAAG
    CTGGTGGCGG TTGTGGCTAG AGCTTCGCGG ACCATTTTTC AGCTACCGCG TGGCAATCTG
    GAAGCGATCA ATCCACGCGT GCTATCGCTC TGTCTGATTG CAAAACATCT CGACGCTCTC
    GAGTACTACG AAGCGTTCGA TATTATGCGC AAGGAGCGTA TCAATTTGAA TCTGCTCGTC
    GATCACGATC CGGCCCGCTT TCTGCAGCAT CTGGCGAGTC ACTTCCTGCG GCAGATAACG
    AACGTGAACT GGTTGAACCT TTTCATATCG GATCTGGCAA ACCAGGATGC GTGCAAAATG
    TACGAAAGTA ACTATCTCGA CCGTGGCACC GGTGGTGGTT CGCTGCCGGA CGGTTACACC
    ATTGCGGAAA AGGTGCGTTT TTGCTGTGAC CGATTGCTCG CAGCGATGGA TTCGGAGCCG
    ACCGTGCTAA CGCTTCCAAA AATCACCTGC CACGTAAAGC AGGGACAACT GGAAAACGCA
    CTGGCACTCA TATGGACCTT GAAGCAGCAA CAGCAATCGC CCGAAGCTGC AGAGGAAGCG
    TTACGCTATC TGCTTTATCT GGTCGAGGTG AACGTACTGT ACGATGTGGC GCTAGGCATG
    TACGATTTCG GGCTCGTCCT GTTTGTAGCG ACCAAGTCGC AGAAAGATCC GAAGGAGTAT
    CTTCCCTTCC TGAACGAGCT GAAGCGGATG GAGGAAAACT ATCGAAAGTA TCGCATCGAT
    TGCCACCTGA AGCGTTACGA TCGTGCGCTA GAACACATTG CCCGGTACGA GACGGACGAG
    GACCGGTTTC GGGAGGCACT GGAGCTGATC ACGACCCACC AGCTGTATGG GGTCGCTCTG
    CGCTGCTACA GGGATAGTGC GAATCAGGAC CACTACCGTC GAGTTTGCGC TGTGTATGGT
    GATTATCTGC GAAAGGGTAG CAAACATGCG AACGCTAGTT TGATGTACGA ACGGGCCGGA
    GATCTGCAGC AGGCCATCTC TTCCGCACGC CATGCACTGG ATTGGCGACG CGTTGTACGG
    TTGTCTACCG GCTCTTCGAT GGCGGTGGAA GCCGTGCTGC GATCTCTTGT GCCGGGTTTG
    CTGGAAGCCG GCGAATATGA TGCAGCAGCC ACGATCGCCC ACGATCATCT GAACGATGCA
    CGGCTAGCGA TTGAGTGTCT GGTGAAGGAT CATCGTTACG AGCGAGCACT TTTGCTAGCC
    ACCGAACCCG AGCTGCACGA GACGGTTCGG AGTAGTGTGG GAGAATATTT AACTACACTG
    ATCGAAACAC TCGGCTCAGA AAAGGAACAA TTTCTGCGCC AAAAAGACCG GCTAGCAACG
    GTGCGGGACG ACCGAATCCG GAAACAATCG GCTGCCGCAG CAGCTGGCGA TCTTGACGAT
    TTGGATGGAG ACTGTGGTGA TTGTGATCTC TTCTCCGATA GCTCCACCCT CGCTTCATCG
    CGTCACACGG GCACATCTGG TCGTTCAGGA AAGTCGCATC GTTCCAGCAA AAATCGTCGC
    AAGCACGAAC GGAAGCTGCT AAACCTGAAG GAAGGCAATC CGTTCGAGGA TATCGCCCTT
    ATCGATGCAC TACACTCGCT GGTCGTGCGT GTGTGCGGAG TCGAGCGACA GCGACACGTA
    CGCTCCATTT GCCAGGTGGC GATGGAACTA TGCTACGATG ACGAGGCGCA CCAGCTGCAG
    CAAGAATATG GTGCACTGTT TCAGCTGATT CGCTACTCGC TGGATGCGAT TTGGGTGCCG
    GAAATGATCG TGCCAGGAAT GCCGCCGACC ATCGGCAGTG GCGGCGATAC GGCCGCGATC
    GGTGATGTCC CAGCGACGAT TCTGACACCG GCGGACCTTG TGAAAGCGCA GGACTCCCAG
    CGGTACTCCT TGATAAAGCC TCATCAACGG TACAAGCCCG AGGTGCAGTC GATAGCCTGG
    CAGTGGGATA TTTTAAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_318429.4
    CDS1..3981
    Translation

    Target ORF information:

    RefSeq Version XM_318429.4
    Organism Anopheles gambiae str. PEST
    Definition Anopheles gambiae str. PEST AGAP003979-RA (AgaP_AGAP003979), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_318429.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGGAACC TGTACCGTGT GTCACACTCT CGCTTCGACC TATCGGAAAA TGCGCTCCGT 
    TCAGTTCGGT CCGGTTTTGC GATCGATCAA AACACGGACC ACAAGTTTTA CTTCGTGCAG
    GACAGTGGTG TGTACTGCTG TCCGCTGTTC GTTGACCGGG AGCATCCGTT CTGCGAACCG
    CTGCTGTCGC AATCGGACGG ATACGAGAAC ATCATAGCGC TGGAACATCT GGCCCTGTCC
    GACGAGCTTT GCTGTGCGTC GTCGAGTGGA AACGTGTGGT CCTGCAAGCT GGAGACGGGA
    GTGGTGGAAG AAGTCACGCA CTGCAAGCAC GGCATCAGGG CGATGCAGTG GAGCCCGGAC
    CAGGAGCTGG TCGTGTTTGT GGACGGCGAG CTGAACGTTG TCACCATGAT CGGGAGCGAG
    TTCGAGCCGA TCAACGAGGT ACAGCTGAAG GACGACACGT TCGGCGACCG GCAGTTCATG
    AGTGTGGGCT GGGGCAAGAA GGAAACTCAA TTTCACGGAT CGGAGGGAAA GGCTGCCGCA
    AAGAAGGGTA CCACGCCCAG CGAGCCGAGC ACAGCGGAGA CGGAGCGACA GCTGGACGCG
    ACCGTAAACA TCAGCTGGCG TGCGGATGGA GAATACTTTG CGGTGGGCTA CCTGGCACCG
    TTTGGTCAGC GAGCTTTCAA AGTGTTCAAC AAGGAGGGCG CATTGCAATA CACGTCGGAA
    AAGTGTCACG GGCTGGAGGG TTCGCTTGCC TGGCGTCCGT CGGGCAATTG GATTGCGATC
    CCGCAGGAAC TTAAAGATCG GTATGTTGTA GCGTTGTTCG AGAAGAATGG GCTGCGACAC
    CGGGAGATTC CGCTGACGTT GAACCCTCGC GCCGGTCAAA GCGTACAGTC GCTTCACTGG
    AGTGCGGACT CGGACGTGCT CGCCGTGCAT TGGGTGGATG GGGAGCAAGG GACTACCGGC
    GTGTTGCTGT ACGTGATCGG AAACTATCAT TGGTACTTGA AGCAAAGCTT GACCTACCCG
    CCTGGTACCG CGCTAACTGG ACTGCAGTGG GATCAGCGTC ACACGGCCGG CAAAACCCTC
    CATTTGTTTT CATCTGACCC AGGCGTTTAT GAGTGCATCC GGTTCGATTT TCGAGTGGAT
    CGTTCCGTGG GCCTTTCGGA AGAAGATCAA ACGATGGTAG CGGTGATCGA TGGAAAGCGA
    CTGCTGTTGA CCGGTTTCCG GCAGGCTGTC ATACCGCCGC CCATGTGTGG CTTTACGCTG
    GAGCAAGAGC ATCCCATCAA TGCGACCGGG TTCATTCGTA CAGCCGGACG CTGCGAAGCA
    TTAGGGCAGG ACATAAGCTC AAACGCCTTC TTTACCGTGG ATTGCCGTGG TGAAATCATT
    TTGTACGATG CTGTATTTAC CAAGACAGCG GGTAGATCCA TATTATCTGG AGTTACCGTA
    TTGCTGAAGA TTTCACCAGA ACAGCTGAAA ATGTTCAACG AAGATTTTCC CCAACAGCAC
    CACTGCGGAC TTCACTACCT TTGGTTGAAC GCGGATAATT TCGTGATGGA TCACAGTCAA
    CTACGTTGCG GCGTTTATAC CATTGCAATG CACAATGACG TCCCCGGCTT GAAGAAAGTA
    TGGCACATGG AGATGGAAAA CAGGGAGGAA ATCGGTTGCA TTGAATCGTC CGGAGCGGAT
    AGTATTCTTG TTGAGCTGCT ATCTGGCCAC GTAATGGAGG TAAAAGGATT GACCGGCCAG
    CAGGATGGTG CATTGGAAAC AAGCTCACGC AATCACAGCG TTTTGCCGGT ATTTTGCGAA
    CAACTGTTTG TCGATCGCAA CCGTCCCGAG CGGACAGTGT ACGCGCTGGC GCAGAACCGC
    CGTCACCTGT ACCGTGATGG AAGTCTTCTC GCGTCGGACA TAACGTCTGC TCTGCTTACC
    GACAGCTACC TACTTTGCAC CACTATTTCG GAGCTTAAAT TCATCGCACT CGGTGCAGCG
    GTTGTGGCAC CCGGCCGTGA TCCGATCATC GGCGAGCGAA GGGTCGAACG TGGCTCGAAG
    CTGGTGGCGG TTGTGGCTAG AGCTTCGCGG ACCATTTTTC AGCTACCGCG TGGCAATCTG
    GAAGCGATCA ATCCACGCGT GCTATCGCTC TGTCTGATTG CAAAACATCT CGACGCTCTC
    GAGTACTACG AAGCGTTCGA TATTATGCGC AAGGAGCGTA TCAATTTGAA TCTGCTCGTC
    GATCACGATC CGGCCCGCTT TCTGCAGCAT CTGGCGAGTC ACTTCCTGCG GCAGATAACG
    AACGTGAACT GGTTGAACCT TTTCATATCG GATCTGGCAA ACCAGGATGC GTGCAAAATG
    TACGAAAGTA ACTATCTCGA CCGTGGCACC GGTGGTGGTT CGCTGCCGGA CGGTTACACC
    ATTGCGGAAA AGGTGCGTTT TTGCTGTGAC CGATTGCTCG CAGCGATGGA TTCGGAGCCG
    ACCGTGCTAA CGCTTCCAAA AATCACCTGC CACGTAAAGC AGGGACAACT GGAAAACGCA
    CTGGCACTCA TATGGACCTT GAAGCAGCAA CAGCAATCGC CCGAAGCTGC AGAGGAAGCG
    TTACGCTATC TGCTTTATCT GGTCGAGGTG AACGTACTGT ACGATGTGGC GCTAGGCATG
    TACGATTTCG GGCTCGTCCT GTTTGTAGCG ACCAAGTCGC AGAAAGATCC GAAGGAGTAT
    CTTCCCTTCC TGAACGAGCT GAAGCGGATG GAGGAAAACT ATCGAAAGTA TCGCATCGAT
    TGCCACCTGA AGCGTTACGA TCGTGCGCTA GAACACATTG CCCGGTACGA GACGGACGAG
    GACCGGTTTC GGGAGGCACT GGAGCTGATC ACGACCCACC AGCTGTATGG GGTCGCTCTG
    CGCTGCTACA GGGATAGTGC GAATCAGGAC CACTACCGTC GAGTTTGCGC TGTGTATGGT
    GATTATCTGC GAAAGGGTAG CAAACATGCG AACGCTAGTT TGATGTACGA ACGGGCCGGA
    GATCTGCAGC AGGCCATCTC TTCCGCACGC CATGCACTGG ATTGGCGACG CGTTGTACGG
    TTGTCTACCG GCTCTTCGAT GGCGGTGGAA GCCGTGCTGC GATCTCTTGT GCCGGGTTTG
    CTGGAAGCCG GCGAATATGA TGCAGCAGCC ACGATCGCCC ACGATCATCT GAACGATGCA
    CGGCTAGCGA TTGAGTGTCT GGTGAAGGAT CATCGTTACG AGCGAGCACT TTTGCTAGCC
    ACCGAACCCG AGCTGCACGA GACGGTTCGG AGTAGTGTGG GAGAATATTT AACTACACTG
    ATCGAAACAC TCGGCTCAGA AAAGGAACAA TTTCTGCGCC AAAAAGACCG GCTAGCAACG
    GTGCGGGACG ACCGAATCCG GAAACAATCG GCTGCCGCAG CAGCTGGCGA TCTTGACGAT
    TTGGATGGAG ACTGTGGTGA TTGTGATCTC TTCTCCGATA GCTCCACCCT CGCTTCATCG
    CGTCACACGG GCACATCTGG TCGTTCAGGA AAGTCGCATC GTTCCAGCAA AAATCGTCGC
    AAGCACGAAC GGAAGCTGCT AAACCTGAAG GAAGGCAATC CGTTCGAGGA TATCGCCCTT
    ATCGATGCAC TACACTCGCT GGTCGTGCGT GTGTGCGGAG TCGAGCGACA GCGACACGTA
    CGCTCCATTT GCCAGGTGGC GATGGAACTA TGCTACGATG ACGAGGCGCA CCAGCTGCAG
    CAAGAATATG GTGCACTGTT TCAGCTGATT CGCTACTCGC TGGATGCGAT TTGGGTGCCG
    GAAATGATCG TGCCAGGAAT GCCGCCGACC ATCGGCAGTG GCGGCGATAC GGCCGCGATC
    GGTGATGTCC CAGCGACGAT TCTGACACCG GCGGACCTTG TGAAAGCGCA GGACTCCCAG
    CGGTACTCCT TGATAAAGCC TCATCAACGG TACAAGCCCG AGGTGCAGTC GATAGCCTGG
    CAGTGGGATA TTTTAAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The Anopheles gambiae genome: an update.
    Trends in parasitology20(2)49-52(2004 Feb)
    Mongin E,Louis C,Holt RA,Birney E,Collins FH


    The genome sequence of the malaria mosquito Anopheles gambiae.
    Science (New York, N.Y.)298(5591)129-49(2002 Oct)
    Holt RA,Subramanian GM,Halpern A,Sutton GG,Charlab R,Nusskern DR,Wincker P,Clark AG,Ribeiro JM,Wides R,Salzberg SL,Loftus B,Yandell M,Majoros WH,Rusch DB,Lai Z,Kraft CL,Abril JF,Anthouard V,Arensburger P,Atkinson PW,Baden H,de Berardinis V,Baldwin D,Benes V,Biedler J,Blass C,Bolanos R,Boscus D,Barnstead M,Cai S,Center A,Chaturverdi K,Christophides GK,Chrystal MA,Clamp M,Cravchik A,Curwen V,Dana A,Delcher A,Dew I,Evans CA,Flanigan M,Grundschober-Freimoser A,Friedli L,Gu Z,Guan P,Guigo R,Hillenmeyer ME,Hladun SL,Hogan JR,Hong YS,Hoover J,Jaillon O,Ke Z,Kodira C,Kokoza E,Koutsos A,Letunic I,Levitsky A,Liang Y,Lin JJ,Lobo NF,Lopez JR,Malek JA,McIntosh TC,Meister S,Miller J,Mobarry C,Mongin E,Murphy SD,O'Brochta DA,Pfannkoch C,Qi R,Regier MA,Remington K,Shao H,Sharakhova MV,Sitter CD,Shetty J,Smith TJ,Strong R,Sun J,Thomasova D,Ton LQ,Topalis P,Tu Z,Unger MF,Walenz B,Wang A,Wang J,Wang M,Wang X,Woodford KJ,Wortman JR,Wu M,Yao A,Zdobnov EM,Zhang H,Zhao Q,Zhao S,Zhu SC,Zhimulev I,Coluzzi M,della Torre A,Roth CW,Louis C,Kalush F,Mural RJ,Myers EW,Adams MD,Smith HO,Broder S,Gardner MJ,Fraser CM,Birney E,Bork P,Brey PT,Venter JC,Weissenbach J,Kafatos FC,Collins FH,Hoffman SL