IKI3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	IKI3
Entrez Gene ID	851100
Full Name	Elongator subunit IKI3
Synonyms	ELP1,KTI7,TOT1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182273.3	NP_013488.3	Elongator subunit IKI3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	IKBKAP	XP_001143066.2
	LOC713524	XP_002808145.1
Rattus norvegicus (Norway rat)	Ikbkap	NP_543175.1
Kluyveromyces lactis	KLLA0E05237g	XP_454181.1
Magnaporthe oryzae (rice blast fungus)	MGG_07174	XP_003715376.1
Bos taurus (cattle)	IKBKAP	XP_003586478.2
Gallus gallus (chicken)	IKBKAP	XP_003643119.1
Danio rerio (zebrafish)	ikbkap	XP_689534.5
Drosophila melanogaster (fruit fly)	Elp1	NP_001262478.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003979	XP_318429.4
Xenopus tropicalis (tropical clawed frog)	ikbkap	NP_001123686.1
Eremothecium gossypii	AGOS_AER047C	NP_984907.2
Schizosaccharomyces pombe (fission yeast)	iki3	NP_595335.1
Homo sapiens (human)	IKBKAP	NP_003631.2
Canis lupus familiaris (dog)	IKBKAP	XP_005626959.1
Arabidopsis thaliana (thale cress)	ABO1	NP_196872.1
Oryza sativa (rice)	Os07g0563700	NP_001059998.1
Mus musculus (house mouse)	Ikbkap	NP_080355.2
Saccharomyces cerevisiae (baker's yeast)	IKI3	NP_013488.3
Neurospora crassa	NCU01486	XP_956763.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

N-terminal truncation of Elp1p is not likely to regulate elongator complex activity.
Title: Yeast Elongator protein Elp1p does not undergo proteolytic processing in exponentially growing cells.

The formation of the Elp1 dimer contributes to its stability in vitro and in vivo and is required for the assembly of yeast Elongator complexes.
Title: Dimerization of elongator protein 1 is essential for Elongator complex assembly.

Thus the conserved basic region in Elp1 may be essential for tRNA wobble uridine modification by acting as tRNA binding motif.
Title: A conserved and essential basic region mediates tRNA binding to the Elp1 subunit of the Saccharomyces cerevisiae Elongator complex.

These data suggest that rather than directly targeting Elp1, the Hrr25 kinase indirectly affects Elp1 phosphorylation states through control of Sit4-dependent dephosphorylation of Elp1.
Title: Elongator function depends on antagonistic regulation by casein kinase Hrr25 and protein phosphatase Sit4.

The following IKI3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IKI3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00699	NM_001182273.3 Latest version!	Saccharomyces cerevisiae S288C Elongator subunit IKI3 (IKI3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IKI3 ( NM_001182273.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013488.3 Saccharomyces cerevisiae S288C Elongator subunit IKI3 (IKI3), partial mRNA.

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CloneID	OSi00699
Clone ID Related Accession (Same CDS sequence)	NM_001182273.3
Accession Version	NM_001182273.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Elongator subunit IKI3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). On Jul 30, 2012 this sequence version replaced NM_001182273.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGTTGAAC ATGACAAGAG TGGGTCAAAG AGGCAGGAGC TAAGATCAAA TATGCGTAAT 
CTTATTACCT TGAATAAGGG TAAATTTAAG CCAACGGCGT CTACTGCAGA AGGCGATGAA 
GATGACTTAT CTTTCACTTT GTTAGACTCG GTATTTGATA CATTATCCGA CAGTATAACA 
TGTGTACTCG GTTCTACGGA TATTGGTGCT ATTGAAGTGC AACAATTCAT GAAGGATGGT 
TCCAGGAATG TGTTGGCTTC ATTCAACATT CAGACTTTTG ACGACAAATT ATTATCTTTT 
GTTCATTTTG CGGATATTAA CCAATTAGTC TTTGTCTTTG AACAAGGTGA TATCATTACA 
GCCACTTATG ATCCTGTCAG TTTGGACCCA GCTGAAACTT TAATTGAAAT TATGGGTACT 
ATAGACAACG GAATTGCAGC CGCGCAGTGG TCCTATGACG AAGAGACGCT GGCCATGGTG 
ACTAAAGATC GTAACGTGGT AGTTCTAAGT AAACTGTTCG AACCCATATC GGAATACCAT 
TTAGAGGTAG ATGATCTTAA GATTTCAAAG CACGTCACCG TTGGTTGGGG TAAAAAGGAA 
ACACAATTCA GAGGTAAAGG TGCACGTGCC ATGGAAAGAG AAGCATTAGC CAGCTTAAAG 
GCATCAGGTT TGGTAGGTAA CCAATTAAGA GATCCGACCA TGCCCTATAT GGTAGATACT 
GGTGATGTAA CCGCATTGGA CAGTCATGAA ATCACAATTT CTTGGAGAGG GGATTGTGAT 
TATTTTGCTG TTTCTTCTGT AGAGGAGGTA CCAGACGAAG ATGATGAAAC AAAATCAATA 
AAAAGAAGAG CTTTCAGAGT TTTTTCCCGT GAAGGCCAAT TGGATAGTGC TTCTGAGCCA 
GTAACTGGGA TGGAACACCA GTTAAGCTGG AAACCACAAG GTTCTTTGAT TGCGTCCATT 
CAAAGAAAAA CCGACCTTGG TGAGGAGGAT TCAGTGGATG TGATTTTCTT CGAACGTAAC 
GGTTTAAGGC ATGGTGAATT TGATACAAGA TTACCTCTAG ATGAGAAGGT AGAAAGTGTA 
TGCTGGAATA GTAACTCAGA GGCACTAGCC GTAGTATTGG CAAACCGCAT TCAGTTGTGG 
ACGTCCAAGA ATTACCATTG GTATTTAAAA CAAGAATTGT ACGCTAGCGA CATTTCCTAC 
GTTAAATGGC ACCCAGAAAA GGACTTCACG TTGATGTTTT CTGATGCTGG ATTTATCAAT 
ATCGTTGATT TCGCTTATAA GATGGCGCAG GGTCCGACAT TAGAGCCGTT CGATAACGGT 
ACCTCTCTTG TTGTTGATGG AAGGACAGTG AATATCACTC CTCTAGCTCT TGCTAATGTC 
CCTCCTCCAA TGTACTATCG TGATTTTGAA ACTCCAGGAA ATGTATTGGA TGTAGCGTGC 
AGTTTTTCGA ATGAAATATA TGCAGCAATC AACAAAGATG TTCTAATTTT CGCAGCCGTC 
CCAAGTATCG AAGAAATGAA AAAAGGCAAA CATCCAAGTA TAGTCTGCGA ATTCCCCAAA 
TCTGAGTTCA CAAGTGAGGT TGACTCTTTA AGGCAAGTTG CATTTATTAA CGATTCCATT 
GTCGGTGTGT TGCTTGACAC AGATAACCTT TCTAGAATAG CGCTTTTGGA TATCCAGGAT 
ATTACGCAAC CAACTTTAAT AACTATTGTG GAGGTCTATG ATAAAATCGT TTTACTAAGA 
AGTGACTTTG ATTATAACCA CTTGGTGTAT GAAACTCGCG ATGGTACAGT TTGCCAATTG 
GATGCGGAAG GCCAGTTAAT GGAAATTACC AAGTTTCCTC AATTGGTACG TGATTTTAGA 
GTAAAAAGAG TCCATAACAC ATCAGCAGAA GATGATGACA ACTGGTCTGC AGAAAGTTCT 
GAGTTAGTTG CATTTGGTAT CACAAATAAC GGTAAGTTAT TTGCTAATCA AGTTCTTTTG 
GCTTCGGCAG TTACTTCCTT GGAGATTACC GATTCCTTTC TGTTGTTCAC CACGGCACAA 
CACAATTTAC AGTTTGTGCA TTTAAATTCC ACCGATTTCA AGCCATTGCC TTTGGTAGAG 
GAGGGTGTGG AAGATGAAAG AGTGCGTGCC ATTGAAAGAG GTTCCATTTT AGTGTCCGTT 
ATTCCATCTA AGTCTTCCGT TGTTTTGCAA GCCACACGTG GTAATCTAGA AACAATTTAT 
CCAAGAATTA TGGTACTGGC AGAGGTAAGA AAGAATATCA TGGCAAAACG TTATAAAGAG 
GCGTTTATTG TTTGTCGTAC ACACAGAATT AACCTTGATA TTTTGCATGA CTATGCGCCA 
GAGTTATTTA TTGAAAACTT AGAAGTATTT ATCAACCAAA TCGGAAGGGT TGATTATCTC 
AATTTATTTA TTTCCTGTTT GTCGGAAGAT GACGTTACCA AAACAAAATA TAAGGAAACC 
CTTTATTCAG GTATCTCGAA GTCATTTGGA ATGGAACCAG CACCACTGAC TGAAATGCAA 
ATATATATGA AAAAGAAAAT GTTTGATCCC AAAACCTCTA AAGTCAACAA AATATGCGAT 
GCTGTGCTAA ATGTTTTATT AAGCAATCCT GAATACAAGA AGAAATACTT GCAAACGATC 
ATTACAGCAT ATGCATCCCA AAACCCTCAA AACTTATCGG CTGCACTAAA ACTAATTAGT 
GAACTAGAAA ATTCTGAAGA AAAAGACTCT TGTGTAACTT ACTTGTGTTT TTTGCAAGAC 
GTCAACGTGG TTTATAAATC TGCCCTATCT TTGTATGATG TCAGTTTAGC ATTATTAGTC 
GCCCAAAAAT CACAAATGGA TCCTCGTGAA TATCTGCCAT TTTTACAAGA ATTACAAGAT 
AATGAGCCTT TACGCCGTAA GTTTTTGATT GATGATTACT TAGGGAACTA TGAAAAAGCT 
TTGGAACACT TGTCCGAAAT TGATAAAGAT GGTAATGTTT CAGAGGAAGT CATAGACTAT 
GTTGAATCTC ATGATTTGTA TAAGCATGGT TTAGCACTTT ACCGTTATGA TTCTGAGAAG 
CAGAATGTTA TTTATAACAT TTACGCAAAG CATTTGTCAT CAAATCAAAT GTATACGGAT 
GCGGCGGTGG CGTACGAGAT GCTAGGAAAG CTCAAAGAAG CAATGGGGGC ATACCAATCA 
GCGAAAAGAT GGCGTGAGGC CATGTCCATC GCTGTTCAGA AATTCCCAGA GGAAGTTGAG 
TCAGTCGCAG AAGAGTTGAT ATCTTCTTTG ACTTTTGAAC ATAGATATGT TGATGCTGCT 
GATATTCAAT TGGAATATTT GGACAATGTT AAAGAAGCCG TAGCTTTGTA CTGTAAGGCC 
TATAGATATG ATATTGCTTC TCTTGTGGCG ATAAAGGCCA AGAAAGATGA ACTCTTGGAA 
GAAGTTGTCG ATCCCGGTTT AGGCGAAGGT TTTGGTATAA TTGCAGAATT ACTCGCCGAT 
TGTAAAGGCC AGATTAATTC ACAGTTAAGA AGATTGAGAG AGCTACGCGC GAAGAAGGAG 
GAGAACCCAT ACGCCTTTTA TGGCCAGGAA ACTGAGCAAG CGGATGACGT TTCTGTTGCT 
CCTTCTGAGA CGTCAACACA AGAATCATTC TTCACCAGAT ACACAGGCAA AACTGGGGGT 
ACTGCAAAGA CTGGTGCTTC CAGACGTACA GCTAAAAACA AGCGTAGGGA AGAGCGTAAG 
CGTGCTCGTG GTAAGAAGGG AACTATTTAT GAAGAAGAAT ATTTGGTTCA GTCAGTCGGA 
AGATTGATTG AGAGGTTAAA CCAAACAAAA CCAGACGCTG TGAGAGTTGT TGAGGGTCTT 
TGTAGACGAA ACATGAGAGA GCAAGCTCAC CAAATTCAAA AGAACTTTGT TGAAGTACTT 
GATTTATTGA AGGCTAACGT TAAAGAAATT TATTCTATTA GCGAAAAGGA TAGAGAAAGG 
GTTAATGAAA ATGGGGAAGT ATATTATATC CCAGAAATAC CTGTTCCAGA GATTCATGAT 
TTCCCTAAGA GTCATATTGT TGATTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013488.3
CDS	1..4050
Translation	MVEHDKSGSKRQELRSNMRNLITLNKGKFKPTASTAEGDEDDLSFTLLDSVFDTLSDSITCVLGSTDIGAIEVQQFMKDGSRNVLASFNIQTFDDKLLSFVHFADINQLVFVFEQGDIITATYDPVSLDPAETLIEIMGTIDNGIAAAQWSYDEETLAMVTKDRNVVVLSKLFEPISEYHLEVDDLKISKHVTVGWGKKETQFRGKGARAMEREALASLKASGLVGNQLRDPTMPYMVDTGDVTALDSHEITISWRGDCDYFAVSSVEEVPDEDDETKSIKRRAFRVFSREGQLDSASEPVTGMEHQLSWKPQGSLIASIQRKTDLGEEDSVDVIFFERNGLRHGEFDTRLPLDEKVESVCWNSNSEALAVVLANRIQLWTSKNYHWYLKQELYASDISYVKWHPEKDFTLMFSDAGFINIVDFAYKMAQGPTLEPFDNGTSLVVDGRTVNITPLALANVPPPMYYRDFETPGNVLDVACSFSNEIYAAINKDVLIFAAVPSIEEMKKGKHPSIVCEFPKSEFTSEVDSLRQVAFINDSIVGVLLDTDNLSRIALLDIQDITQPTLITIVEVYDKIVLLRSDFDYNHLVYETRDGTVCQLDAEGQLMEITKFPQLVRDFRVKRVHNTSAEDDDNWSAESSELVAFGITNNGKLFANQVLLASAVTSLEITDSFLLFTTAQHNLQFVHLNSTDFKPLPLVEEGVEDERVRAIERGSILVSVIPSKSSVVLQATRGNLETIYPRIMVLAEVRKNIMAKRYKEAFIVCRTHRINLDILHDYAPELFIENLEVFINQIGRVDYLNLFISCLSEDDVTKTKYKETLYSGISKSFGMEPAPLTEMQIYMKKKMFDPKTSKVNKICDAVLNVLLSNPEYKKKYLQTIITAYASQNPQNLSAALKLISELENSEEKDSCVTYLCFLQDVNVVYKSALSLYDVSLALLVAQKSQMDPREYLPFLQELQDNEPLRRKFLIDDYLGNYEKALEHLSEIDKDGNVSEEVIDYVESHDLYKHGLALYRYDSEKQNVIYNIYAKHLSSNQMYTDAAVAYEMLGKLKEAMGAYQSAKRWREAMSIAVQKFPEEVESVAEELISSLTFEHRYVDAADIQLEYLDNVKEAVALYCKAYRYDIASLVAIKAKKDELLEEVVDPGLGEGFGIIAELLADCKGQINSQLRRLRELRAKKEENPYAFYGQETEQADDVSVAPSETSTQESFFTRYTGKTGGTAKTGASRRTAKNKRREERKRARGKKGTIYEEEYLVQSVGRLIERLNQTKPDAVRVVEGLCRRNMREQAHQIQKNFVEVLDLLKANVKEIYSISEKDRERVNENGEVYYIPEIPVPEIHDFPKSHIVDF

Target ORF information:

RefSeq Version	NM_001182273.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Elongator subunit IKI3 (IKI3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182273.3

ORF Insert Sequence:

ATGGTTGAAC ATGACAAGAG TGGGTCAAAG AGGCAGGAGC TAAGATCAAA TATGCGTAAT 
CTTATTACCT TGAATAAGGG TAAATTTAAG CCAACGGCGT CTACTGCAGA AGGCGATGAA 
GATGACTTAT CTTTCACTTT GTTAGACTCG GTATTTGATA CATTATCCGA CAGTATAACA 
TGTGTACTCG GTTCTACGGA TATTGGTGCT ATTGAAGTGC AACAATTCAT GAAGGATGGT 
TCCAGGAATG TGTTGGCTTC ATTCAACATT CAGACTTTTG ACGACAAATT ATTATCTTTT 
GTTCATTTTG CGGATATTAA CCAATTAGTC TTTGTCTTTG AACAAGGTGA TATCATTACA 
GCCACTTATG ATCCTGTCAG TTTGGACCCA GCTGAAACTT TAATTGAAAT TATGGGTACT 
ATAGACAACG GAATTGCAGC CGCGCAGTGG TCCTATGACG AAGAGACGCT GGCCATGGTG 
ACTAAAGATC GTAACGTGGT AGTTCTAAGT AAACTGTTCG AACCCATATC GGAATACCAT 
TTAGAGGTAG ATGATCTTAA GATTTCAAAG CACGTCACCG TTGGTTGGGG TAAAAAGGAA 
ACACAATTCA GAGGTAAAGG TGCACGTGCC ATGGAAAGAG AAGCATTAGC CAGCTTAAAG 
GCATCAGGTT TGGTAGGTAA CCAATTAAGA GATCCGACCA TGCCCTATAT GGTAGATACT 
GGTGATGTAA CCGCATTGGA CAGTCATGAA ATCACAATTT CTTGGAGAGG GGATTGTGAT 
TATTTTGCTG TTTCTTCTGT AGAGGAGGTA CCAGACGAAG ATGATGAAAC AAAATCAATA 
AAAAGAAGAG CTTTCAGAGT TTTTTCCCGT GAAGGCCAAT TGGATAGTGC TTCTGAGCCA 
GTAACTGGGA TGGAACACCA GTTAAGCTGG AAACCACAAG GTTCTTTGAT TGCGTCCATT 
CAAAGAAAAA CCGACCTTGG TGAGGAGGAT TCAGTGGATG TGATTTTCTT CGAACGTAAC 
GGTTTAAGGC ATGGTGAATT TGATACAAGA TTACCTCTAG ATGAGAAGGT AGAAAGTGTA 
TGCTGGAATA GTAACTCAGA GGCACTAGCC GTAGTATTGG CAAACCGCAT TCAGTTGTGG 
ACGTCCAAGA ATTACCATTG GTATTTAAAA CAAGAATTGT ACGCTAGCGA CATTTCCTAC 
GTTAAATGGC ACCCAGAAAA GGACTTCACG TTGATGTTTT CTGATGCTGG ATTTATCAAT 
ATCGTTGATT TCGCTTATAA GATGGCGCAG GGTCCGACAT TAGAGCCGTT CGATAACGGT 
ACCTCTCTTG TTGTTGATGG AAGGACAGTG AATATCACTC CTCTAGCTCT TGCTAATGTC 
CCTCCTCCAA TGTACTATCG TGATTTTGAA ACTCCAGGAA ATGTATTGGA TGTAGCGTGC 
AGTTTTTCGA ATGAAATATA TGCAGCAATC AACAAAGATG TTCTAATTTT CGCAGCCGTC 
CCAAGTATCG AAGAAATGAA AAAAGGCAAA CATCCAAGTA TAGTCTGCGA ATTCCCCAAA 
TCTGAGTTCA CAAGTGAGGT TGACTCTTTA AGGCAAGTTG CATTTATTAA CGATTCCATT 
GTCGGTGTGT TGCTTGACAC AGATAACCTT TCTAGAATAG CGCTTTTGGA TATCCAGGAT 
ATTACGCAAC CAACTTTAAT AACTATTGTG GAGGTCTATG ATAAAATCGT TTTACTAAGA 
AGTGACTTTG ATTATAACCA CTTGGTGTAT GAAACTCGCG ATGGTACAGT TTGCCAATTG 
GATGCGGAAG GCCAGTTAAT GGAAATTACC AAGTTTCCTC AATTGGTACG TGATTTTAGA 
GTAAAAAGAG TCCATAACAC ATCAGCAGAA GATGATGACA ACTGGTCTGC AGAAAGTTCT 
GAGTTAGTTG CATTTGGTAT CACAAATAAC GGTAAGTTAT TTGCTAATCA AGTTCTTTTG 
GCTTCGGCAG TTACTTCCTT GGAGATTACC GATTCCTTTC TGTTGTTCAC CACGGCACAA 
CACAATTTAC AGTTTGTGCA TTTAAATTCC ACCGATTTCA AGCCATTGCC TTTGGTAGAG 
GAGGGTGTGG AAGATGAAAG AGTGCGTGCC ATTGAAAGAG GTTCCATTTT AGTGTCCGTT 
ATTCCATCTA AGTCTTCCGT TGTTTTGCAA GCCACACGTG GTAATCTAGA AACAATTTAT 
CCAAGAATTA TGGTACTGGC AGAGGTAAGA AAGAATATCA TGGCAAAACG TTATAAAGAG 
GCGTTTATTG TTTGTCGTAC ACACAGAATT AACCTTGATA TTTTGCATGA CTATGCGCCA 
GAGTTATTTA TTGAAAACTT AGAAGTATTT ATCAACCAAA TCGGAAGGGT TGATTATCTC 
AATTTATTTA TTTCCTGTTT GTCGGAAGAT GACGTTACCA AAACAAAATA TAAGGAAACC 
CTTTATTCAG GTATCTCGAA GTCATTTGGA ATGGAACCAG CACCACTGAC TGAAATGCAA 
ATATATATGA AAAAGAAAAT GTTTGATCCC AAAACCTCTA AAGTCAACAA AATATGCGAT 
GCTGTGCTAA ATGTTTTATT AAGCAATCCT GAATACAAGA AGAAATACTT GCAAACGATC 
ATTACAGCAT ATGCATCCCA AAACCCTCAA AACTTATCGG CTGCACTAAA ACTAATTAGT 
GAACTAGAAA ATTCTGAAGA AAAAGACTCT TGTGTAACTT ACTTGTGTTT TTTGCAAGAC 
GTCAACGTGG TTTATAAATC TGCCCTATCT TTGTATGATG TCAGTTTAGC ATTATTAGTC 
GCCCAAAAAT CACAAATGGA TCCTCGTGAA TATCTGCCAT TTTTACAAGA ATTACAAGAT 
AATGAGCCTT TACGCCGTAA GTTTTTGATT GATGATTACT TAGGGAACTA TGAAAAAGCT 
TTGGAACACT TGTCCGAAAT TGATAAAGAT GGTAATGTTT CAGAGGAAGT CATAGACTAT 
GTTGAATCTC ATGATTTGTA TAAGCATGGT TTAGCACTTT ACCGTTATGA TTCTGAGAAG 
CAGAATGTTA TTTATAACAT TTACGCAAAG CATTTGTCAT CAAATCAAAT GTATACGGAT 
GCGGCGGTGG CGTACGAGAT GCTAGGAAAG CTCAAAGAAG CAATGGGGGC ATACCAATCA 
GCGAAAAGAT GGCGTGAGGC CATGTCCATC GCTGTTCAGA AATTCCCAGA GGAAGTTGAG 
TCAGTCGCAG AAGAGTTGAT ATCTTCTTTG ACTTTTGAAC ATAGATATGT TGATGCTGCT 
GATATTCAAT TGGAATATTT GGACAATGTT AAAGAAGCCG TAGCTTTGTA CTGTAAGGCC 
TATAGATATG ATATTGCTTC TCTTGTGGCG ATAAAGGCCA AGAAAGATGA ACTCTTGGAA 
GAAGTTGTCG ATCCCGGTTT AGGCGAAGGT TTTGGTATAA TTGCAGAATT ACTCGCCGAT 
TGTAAAGGCC AGATTAATTC ACAGTTAAGA AGATTGAGAG AGCTACGCGC GAAGAAGGAG 
GAGAACCCAT ACGCCTTTTA TGGCCAGGAA ACTGAGCAAG CGGATGACGT TTCTGTTGCT 
CCTTCTGAGA CGTCAACACA AGAATCATTC TTCACCAGAT ACACAGGCAA AACTGGGGGT 
ACTGCAAAGA CTGGTGCTTC CAGACGTACA GCTAAAAACA AGCGTAGGGA AGAGCGTAAG 
CGTGCTCGTG GTAAGAAGGG AACTATTTAT GAAGAAGAAT ATTTGGTTCA GTCAGTCGGA 
AGATTGATTG AGAGGTTAAA CCAAACAAAA CCAGACGCTG TGAGAGTTGT TGAGGGTCTT 
TGTAGACGAA ACATGAGAGA GCAAGCTCAC CAAATTCAAA AGAACTTTGT TGAAGTACTT 
GATTTATTGA AGGCTAACGT TAAAGAAATT TATTCTATTA GCGAAAAGGA TAGAGAAAGG 
GTTAATGAAA ATGGGGAAGT ATATTATATC CCAGAAATAC CTGTTCCAGA GATTCATGAT 
TTCCCTAAGA GTCATATTGT TGATTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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IKI3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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