AgaP_AGAP006637 cDNA ORF clone, Anopheles gambiae str. PEST

The following AgaP_AGAP006637 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AgaP_AGAP006637 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OAh05528 XM_316668.4
Latest version!
Anopheles gambiae str. PEST AGAP006637-RA (AgaP_AGAP006637), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OAh05527 XM_001237848.2
Latest version!
Anopheles gambiae str. PEST AGAP006637-RB (AgaP_AGAP006637), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OAh05528
    Clone ID Related Accession (Same CDS sequence) XM_316668.4
    Accession Version XM_316668.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-25
    Organism Anopheles gambiae str. PEST
    Product AGAP006637-PA
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_078265). On Oct 15, 2007 this sequence version replaced XM_316668.3. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAACGGTA GTTTGGCGAA CGGATTGAAC GAGGGCCACC TGAACGCGCC GCCGACGAAG 
    ATCGCCTTCG ATCGGAGCAT TTCCGCGTAC AGCGAGCGGG ATGTGCAGTG CGGGTTTTGG
    GTGTGCCGTG GCCCTTTCTG GCAGCGGCTG GCCAGCAAGA AGCTGTACGT GCTGCTGTTC
    GGGCTGATGG GATGCATCCT CAACTCGACC ACCTCCTACT TCTACGGCAC GCTCACGACG
    GTGGAGAAGA GCATACAGAT CTCGTCCCGC ACGGTGGGTA TCATTACCGC CGGCTCCGAC
    CTATCCTTCG TGCTGGCATC GCTCTTTCTA TCGTACTACG CCTCGAACCG CCGCAAACCG
    CTATGGATAG CGATGGGGAT TGTCAGCATG GCACTATCCT GCTTCGTGAA CGCACTGCCC
    CACTTCCTGT TCTCAAAGGC TCAGGCAGAG ATGGAGCAGA ATCTCGGCAA CTTGACGAAG
    GGTGCGGATA ACCTGTGTAG AGAGGATCGA CTATCGCCAG ACTGTTCGCT GGACGTCGGA
    GATATAAGAC CACAGATCGT GCTGTTCTTC GGCAGCTTCC TGTCCGGCAT AGGGACATCA
    CTATATTTCA CCCTCGGCCT TACCTATCTG GATGATAATG TGCGCAAGGA GAAGGTACCG
    TTCCTATCCA GTTTGGCTGC GTTCGGGCGG CGCATGGGAC CGCTGGTTGG CTTTTCGTTG
    GCTTCCCTGT GTTTGAAGTA CTTTGTGCAC GTCGGCGTCG ATCCGGGCTA TAACCAGAGC
    GATCCGCGCT GGATCGGTGC CTGGTGGATG GGTTGGCTAG CGCTCGGCTC CACGCTTATC
    ATCATCGCAC CGTTCTTTGC CAGCTTCCCG AAGGTGCTGC CCAGGACGGC GGAACGCATA
    TCGCTTTCCC GGCAGGCGAG TCGCGCTAGC GGTACCTCGA CCGCCGAAGA GGATGTCGAG
    ACGAACCGGA AGGTGTCGTT CAGTGACATG CTGGTTACGG TGCGCCGGCT CGTCACCAAC
    AAGGCGTACG TGTTTAACAA TGTGGCCAGC ATCTTCTACT TCTTCGGGTA TATGCCGTAC
    TTCCTGTTTC AGGCTAAGTA CATCGAGATC CAGTATCGGC TGACACCGTC CCAGGCTAAC
    ATGATTACGG GCTCTACTTC GCTCGTCTTT TCCGCACTGG GAATACTGAT CGCCGGTATG
    GTGATCCAAA AGCTGCGTGT CAGTTCTAGA CAGCTGGCCG GGTGGAACGT AATCACAAGC
    ATTCTTTCGG CGGCACTCAT CGTTGGGTAT GCGTGGTTCG GTTGCTCTGC GTTGAACAAT
    GCAGATATCG TTGCTAATTC TCAAGACGCA TCCTGTAGTT TGGGATGCAA TTGTGAGTTC
    GTGAAGTACG CCCCAATCTG TGGAAGCGAT GGACAAACGT ATCTTTCGCC GTGTCATGCT
    GGATGCCGGG ACTCGCTCAA GGATCCAACT GGAACAACGG TAGGTGACAC TGCGTTCGTA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_316668.4
    CDS1..1503
    Translation

    Target ORF information:

    RefSeq Version XM_316668.4
    Organism Anopheles gambiae str. PEST
    Definition Anopheles gambiae str. PEST AGAP006637-RA (AgaP_AGAP006637), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_316668.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAACGGTA GTTTGGCGAA CGGATTGAAC GAGGGCCACC TGAACGCGCC GCCGACGAAG 
    ATCGCCTTCG ATCGGAGCAT TTCCGCGTAC AGCGAGCGGG ATGTGCAGTG CGGGTTTTGG
    GTGTGCCGTG GCCCTTTCTG GCAGCGGCTG GCCAGCAAGA AGCTGTACGT GCTGCTGTTC
    GGGCTGATGG GATGCATCCT CAACTCGACC ACCTCCTACT TCTACGGCAC GCTCACGACG
    GTGGAGAAGA GCATACAGAT CTCGTCCCGC ACGGTGGGTA TCATTACCGC CGGCTCCGAC
    CTATCCTTCG TGCTGGCATC GCTCTTTCTA TCGTACTACG CCTCGAACCG CCGCAAACCG
    CTATGGATAG CGATGGGGAT TGTCAGCATG GCACTATCCT GCTTCGTGAA CGCACTGCCC
    CACTTCCTGT TCTCAAAGGC TCAGGCAGAG ATGGAGCAGA ATCTCGGCAA CTTGACGAAG
    GGTGCGGATA ACCTGTGTAG AGAGGATCGA CTATCGCCAG ACTGTTCGCT GGACGTCGGA
    GATATAAGAC CACAGATCGT GCTGTTCTTC GGCAGCTTCC TGTCCGGCAT AGGGACATCA
    CTATATTTCA CCCTCGGCCT TACCTATCTG GATGATAATG TGCGCAAGGA GAAGGTACCG
    TTCCTATCCA GTTTGGCTGC GTTCGGGCGG CGCATGGGAC CGCTGGTTGG CTTTTCGTTG
    GCTTCCCTGT GTTTGAAGTA CTTTGTGCAC GTCGGCGTCG ATCCGGGCTA TAACCAGAGC
    GATCCGCGCT GGATCGGTGC CTGGTGGATG GGTTGGCTAG CGCTCGGCTC CACGCTTATC
    ATCATCGCAC CGTTCTTTGC CAGCTTCCCG AAGGTGCTGC CCAGGACGGC GGAACGCATA
    TCGCTTTCCC GGCAGGCGAG TCGCGCTAGC GGTACCTCGA CCGCCGAAGA GGATGTCGAG
    ACGAACCGGA AGGTGTCGTT CAGTGACATG CTGGTTACGG TGCGCCGGCT CGTCACCAAC
    AAGGCGTACG TGTTTAACAA TGTGGCCAGC ATCTTCTACT TCTTCGGGTA TATGCCGTAC
    TTCCTGTTTC AGGCTAAGTA CATCGAGATC CAGTATCGGC TGACACCGTC CCAGGCTAAC
    ATGATTACGG GCTCTACTTC GCTCGTCTTT TCCGCACTGG GAATACTGAT CGCCGGTATG
    GTGATCCAAA AGCTGCGTGT CAGTTCTAGA CAGCTGGCCG GGTGGAACGT AATCACAAGC
    ATTCTTTCGG CGGCACTCAT CGTTGGGTAT GCGTGGTTCG GTTGCTCTGC GTTGAACAAT
    GCAGATATCG TTGCTAATTC TCAAGACGCA TCCTGTAGTT TGGGATGCAA TTGTGAGTTC
    GTGAAGTACG CCCCAATCTG TGGAAGCGAT GGACAAACGT ATCTTTCGCC GTGTCATGCT
    GGATGCCGGG ACTCGCTCAA GGATCCAACT GGAACAACGG TAGGTGACAC TGCGTTCGTA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OAh05527
    Clone ID Related Accession (Same CDS sequence) XM_001237848.2
    Accession Version XM_001237848.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2064bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-25
    Organism Anopheles gambiae str. PEST
    Product AGAP006637-PB
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_078265). On Oct 15, 2007 this sequence version replaced XM_001237848.1. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAACGGTA GTTTGGCGAA CGGATTGAAC GAGGGCCACC TGAACGCGCC GCCGACGAAG 
    ATCGCCTTCG ATCGGAGCAT TTCCGCGTAC AGCGAGCGGG ATGTGCAGTG CGGGTTTTGG
    GTGTGCCGTG GCCCTTTCTG GCAGCGGCTG GCCAGCAAGA AGCTGTACGT GCTGCTGTTC
    GGGCTGATGG GATGCATCCT CAACTCGACC ACCTCCTACT TCTACGGCAC GCTCACGACG
    GTGGAGAAGA GCATACAGAT CTCGTCCCGC ACGGTGGGTA TCATTACCGC CGGCTCCGAC
    CTATCCTTCG TGCTGGCATC GCTCTTTCTA TCGTACTACG CCTCGAACCG CCGCAAACCG
    CTATGGATAG CGATGGGGAT TGTCAGCATG GCACTATCCT GCTTCGTGAA CGCACTGCCC
    CACTTCCTGT TCTCAAAGGC TCAGGCAGAG ATGGAGCAGA ATCTCGGCAA CTTGACGAAG
    GGTGCGGATA ACCTGTGTAG AGAGGATCGA CTATCGCCAG ACTGTTCGCT GGACGTCGGA
    GATATAAGAC CACAGATCGT GCTGTTCTTC GGCAGCTTCC TGTCCGGCAT AGGGACATCA
    CTATATTTCA CCCTCGGCCT TACCTATCTG GATGATAATG TGCGCAAGGA GAAGGTACCG
    TTCCTATCCA GTTTGGCTGC GTTCGGGCGG CGCATGGGAC CGCTGGTTGG CTTTTCGTTG
    GCTTCCCTGT GTTTGAAGTA CTTTGTGCAC GTCGGCGTCG ATCCGGGCTA TAACCAGAGC
    GATCCGCGCT GGATCGGTGC CTGGTGGATG GGTTGGCTAG CGCTCGGCTC CACGCTTATC
    ATCATCGCAC CGTTCTTTGC CAGCTTCCCG AAGGTGCTGC CCAGGACGGC GGAACGCATA
    TCGCTTTCCC GGCAGGCGAG TCGCGCTAGC GGTACCTCGA CCGCCGAAGA GGATGTCGAG
    ACGAACCGGA AGGTGTCGTT CAGTGACATG CTGGTTACGG TGCGCCGGCT CGTCACCAAC
    AAGGCGTACG TGTTTAACAA TGTGGCCAGC ATCTTCTACT TCTTCGGGTA TATGCCGTAC
    TTCCTGTTTC AGGCTAAGTA CATCGAGATC CAGTATCGGC TGACACCGTC CCAGGCTAAC
    ATGATTACGG GCTCTACTTC GCTCGTCTTT TCCGCACTGG GAATACTGAT CGCCGGTATG
    GTGATCCAAA AGCTGCGTGT CAGTTCTAGA CAGCTGGCCG GGTGGAACGT AATCACAAGC
    ATTCTTTCGG CGGCACTCAT CGTTGGGTAT GCGTGGTTCG GTTGCTCTGC GTTGAACAAT
    GCAGATATCG TTGCTAATTC TCAAGACGCA TCCTGTAGTT TGGGATGCAA TTGTGAGTTC
    GTGAAGTACG CCCCAATCTG TGGAAGCGAT GGACAAACGT ATCTTTCGCC GTGTCATGCT
    GGATGCCGGG ACTCGCTCAA GGATCCAACT GGAACAACGC TCTACACAAA CTGTTCCTGC
    ATCGCGAGCC CGACAGATGA TTTGGATGCC AATTTCACCT CCTCTTATGG TTCGGCCACG
    CCTGGAAGTT GCCCAGTGGA CTGCGGGACA CCGTTCTACT TCTTCCTGAT GTGCTTGTGC
    TTGAACAAGT TTATCGGTGG CACGGAATCG GCTGCAAACT TTCTCATCGG CTTAAGATGT
    GTTGAACCAC GCGATAAAGC CATCTCGATG GGATTCTCGA TGGCGATCAA CACACTGTTC
    TCGTTCCTTC CTGCACCGAT CGTCTTCGGT GCTATCATCG ACCGGACCTG TGTGCTTTGG
    GGCCGTACCT GCGCTAAACA GGGCAACTGC TGGCTGTACG ATGGTGAGGC ACTGCGCGCA
    TCAATGAACT ACACGGCGGC TGTGTTTGTC ATCATTGGAA CCTGCTTCGA TATGGGCACC
    TGGTACTACT CGAAGAACTT TAAGATATTC GACGATAAGC CCGGCGAGCA GAGTGCTAGC
    AAGAGCCCCA CGGAACGGGA GCTAGTGTCG CTCGTTAGTA AAGCGAAGGA AGAGGAGGCA
    CAAACTACTC GTAACAACCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001237849.2
    CDS1..2064
    Translation

    Target ORF information:

    RefSeq Version XM_001237848.2
    Organism Anopheles gambiae str. PEST
    Definition Anopheles gambiae str. PEST AGAP006637-RB (AgaP_AGAP006637), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001237848.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAACGGTA GTTTGGCGAA CGGATTGAAC GAGGGCCACC TGAACGCGCC GCCGACGAAG 
    ATCGCCTTCG ATCGGAGCAT TTCCGCGTAC AGCGAGCGGG ATGTGCAGTG CGGGTTTTGG
    GTGTGCCGTG GCCCTTTCTG GCAGCGGCTG GCCAGCAAGA AGCTGTACGT GCTGCTGTTC
    GGGCTGATGG GATGCATCCT CAACTCGACC ACCTCCTACT TCTACGGCAC GCTCACGACG
    GTGGAGAAGA GCATACAGAT CTCGTCCCGC ACGGTGGGTA TCATTACCGC CGGCTCCGAC
    CTATCCTTCG TGCTGGCATC GCTCTTTCTA TCGTACTACG CCTCGAACCG CCGCAAACCG
    CTATGGATAG CGATGGGGAT TGTCAGCATG GCACTATCCT GCTTCGTGAA CGCACTGCCC
    CACTTCCTGT TCTCAAAGGC TCAGGCAGAG ATGGAGCAGA ATCTCGGCAA CTTGACGAAG
    GGTGCGGATA ACCTGTGTAG AGAGGATCGA CTATCGCCAG ACTGTTCGCT GGACGTCGGA
    GATATAAGAC CACAGATCGT GCTGTTCTTC GGCAGCTTCC TGTCCGGCAT AGGGACATCA
    CTATATTTCA CCCTCGGCCT TACCTATCTG GATGATAATG TGCGCAAGGA GAAGGTACCG
    TTCCTATCCA GTTTGGCTGC GTTCGGGCGG CGCATGGGAC CGCTGGTTGG CTTTTCGTTG
    GCTTCCCTGT GTTTGAAGTA CTTTGTGCAC GTCGGCGTCG ATCCGGGCTA TAACCAGAGC
    GATCCGCGCT GGATCGGTGC CTGGTGGATG GGTTGGCTAG CGCTCGGCTC CACGCTTATC
    ATCATCGCAC CGTTCTTTGC CAGCTTCCCG AAGGTGCTGC CCAGGACGGC GGAACGCATA
    TCGCTTTCCC GGCAGGCGAG TCGCGCTAGC GGTACCTCGA CCGCCGAAGA GGATGTCGAG
    ACGAACCGGA AGGTGTCGTT CAGTGACATG CTGGTTACGG TGCGCCGGCT CGTCACCAAC
    AAGGCGTACG TGTTTAACAA TGTGGCCAGC ATCTTCTACT TCTTCGGGTA TATGCCGTAC
    TTCCTGTTTC AGGCTAAGTA CATCGAGATC CAGTATCGGC TGACACCGTC CCAGGCTAAC
    ATGATTACGG GCTCTACTTC GCTCGTCTTT TCCGCACTGG GAATACTGAT CGCCGGTATG
    GTGATCCAAA AGCTGCGTGT CAGTTCTAGA CAGCTGGCCG GGTGGAACGT AATCACAAGC
    ATTCTTTCGG CGGCACTCAT CGTTGGGTAT GCGTGGTTCG GTTGCTCTGC GTTGAACAAT
    GCAGATATCG TTGCTAATTC TCAAGACGCA TCCTGTAGTT TGGGATGCAA TTGTGAGTTC
    GTGAAGTACG CCCCAATCTG TGGAAGCGAT GGACAAACGT ATCTTTCGCC GTGTCATGCT
    GGATGCCGGG ACTCGCTCAA GGATCCAACT GGAACAACGC TCTACACAAA CTGTTCCTGC
    ATCGCGAGCC CGACAGATGA TTTGGATGCC AATTTCACCT CCTCTTATGG TTCGGCCACG
    CCTGGAAGTT GCCCAGTGGA CTGCGGGACA CCGTTCTACT TCTTCCTGAT GTGCTTGTGC
    TTGAACAAGT TTATCGGTGG CACGGAATCG GCTGCAAACT TTCTCATCGG CTTAAGATGT
    GTTGAACCAC GCGATAAAGC CATCTCGATG GGATTCTCGA TGGCGATCAA CACACTGTTC
    TCGTTCCTTC CTGCACCGAT CGTCTTCGGT GCTATCATCG ACCGGACCTG TGTGCTTTGG
    GGCCGTACCT GCGCTAAACA GGGCAACTGC TGGCTGTACG ATGGTGAGGC ACTGCGCGCA
    TCAATGAACT ACACGGCGGC TGTGTTTGTC ATCATTGGAA CCTGCTTCGA TATGGGCACC
    TGGTACTACT CGAAGAACTT TAAGATATTC GACGATAAGC CCGGCGAGCA GAGTGCTAGC
    AAGAGCCCCA CGGAACGGGA GCTAGTGTCG CTCGTTAGTA AAGCGAAGGA AGAGGAGGCA
    CAAACTACTC GTAACAACCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The Anopheles gambiae genome: an update.
    Trends in parasitology20(2)49-52(2004 Feb)
    Mongin E,Louis C,Holt RA,Birney E,Collins FH


    The genome sequence of the malaria mosquito Anopheles gambiae.
    Science (New York, N.Y.)298(5591)129-49(2002 Oct)
    Holt RA,Subramanian GM,Halpern A,Sutton GG,Charlab R,Nusskern DR,Wincker P,Clark AG,Ribeiro JM,Wides R,Salzberg SL,Loftus B,Yandell M,Majoros WH,Rusch DB,Lai Z,Kraft CL,Abril JF,Anthouard V,Arensburger P,Atkinson PW,Baden H,de Berardinis V,Baldwin D,Benes V,Biedler J,Blass C,Bolanos R,Boscus D,Barnstead M,Cai S,Center A,Chaturverdi K,Christophides GK,Chrystal MA,Clamp M,Cravchik A,Curwen V,Dana A,Delcher A,Dew I,Evans CA,Flanigan M,Grundschober-Freimoser A,Friedli L,Gu Z,Guan P,Guigo R,Hillenmeyer ME,Hladun SL,Hogan JR,Hong YS,Hoover J,Jaillon O,Ke Z,Kodira C,Kokoza E,Koutsos A,Letunic I,Levitsky A,Liang Y,Lin JJ,Lobo NF,Lopez JR,Malek JA,McIntosh TC,Meister S,Miller J,Mobarry C,Mongin E,Murphy SD,O'Brochta DA,Pfannkoch C,Qi R,Regier MA,Remington K,Shao H,Sharakhova MV,Sitter CD,Shetty J,Smith TJ,Strong R,Sun J,Thomasova D,Ton LQ,Topalis P,Tu Z,Unger MF,Walenz B,Wang A,Wang J,Wang M,Wang X,Woodford KJ,Wortman JR,Wu M,Yao A,Zdobnov EM,Zhang H,Zhao Q,Zhao S,Zhu SC,Zhimulev I,Coluzzi M,della Torre A,Roth CW,Louis C,Kalush F,Mural RJ,Myers EW,Adams MD,Smith HO,Broder S,Gardner MJ,Fraser CM,Birney E,Bork P,Brey PT,Venter JC,Weissenbach J,Kafatos FC,Collins FH,Hoffman SL