egfr cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	egfr
Entrez Gene ID	111573600
Full Name	epidermal growth factor receptor
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023277838.1	XP_023133606.1	epidermal growth factor receptor isoform X2
XM_023277837.1	XP_023133605.1	epidermal growth factor receptor isoform X1

Pathways from BioSystems
Gene Ontology

The following egfr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the egfr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm83855	XM_023277837.1 Latest version!	Amphiprion ocellaris epidermal growth factor receptor (egfr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OAm83856	XM_023277838.1 Latest version!	Amphiprion ocellaris epidermal growth factor receptor (egfr), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

egfr ( XM_023277837.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023133605.1 Amphiprion ocellaris epidermal growth factor receptor (egfr), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAm83855
Clone ID Related Accession (Same CDS sequence)	XM_023277837.1
Accession Version	XM_023277837.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	epidermal growth factor receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019372753.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGCG TTTTTCTCAA GTGGATCACC TCTCTGCTCT GGCTGGGCTC CTGCGTTCTG 
GCGGAAAGGA AAGTATGCCA AGGCATCAGC AACCGGTTTA ACCTCCTGGG TTCGAAAGAC 
AACCACTACC TGAACATGGT GAAGACCTAC GGTAACTGCA CCGTGGTCCT GGAGAACCTG 
GAGGTCACCC ACATGGAGGA GCATCGTGAC CTGTCCTTCC TCAGGTCTAT TGAAGAAGTG 
GGTGGCTATG TCATGATTGC CATCAACACA GCATCCAGGA TTTCTCTGGA GAACCTGCGC 
GTCATCAGAG GACATACCCT TTATGAGGGA GAGTTTGCAC TTGTTGTGTT CGGCAATTAT 
AACAAAGAAT CAAATATGGG CACCAATCAG CTTCATCTAA ACAGTCTAAC AGAAATTCTA 
AAAGGAGGCG TGAAGTTTGG GAGAAACCGG CTGTGCAATG TAGAAACAAT ACAGTGGTAT 
GATATTGTCA GTGCCGACAA CAAACCCAAG ATGGAGCTCC CAGTTGCTAG CAACAACCCA 
CTTTGTAGAA GATGTGACTC GAGCTGCTTC AATGGTTCAT GTTGGGCATC TGGTCCTCAA 
AATTGTCAGA CTTTAACAAA GCTGAACTGT GCACAGCAGT GCTCTAAGAG ATGCAAAGGA 
CCTTCACCAA GTGACTGCTG CAATGAGCAT TGCGCCGCAG GATGCACAGG ACCCAGACCG 
ACCGACTGTC TGGCCTGTCG GGACTTCCGG GATGATGGGG TGTGTAAGGA CTCCTGCCCA 
GGCCTCATGC GCTACGATCC CAACCTGCAC CAGCTGGTTC CCAACCCACA TGGAAAGTAC 
AACTTCGGGG CCACCTGTGT CAAAAGCTGC CCACATAATT ATGTTGTGAC TGATCACGGA 
GCCTGTGTGC GGACGTGCAG CGGTAACACA TACGAGGTGG ATGAAGGAGG AATTAGGAAA 
TGCGCCAAGT GTGACGGACT GTGTCCAAAA GTGTGCAATG GACTTGGAAC AGGAGACCTG 
ACTCACGCTG TGTCCATTAA TGCCACAAAC ATCGACTCCT TTAAAAACTG CACCAAAATC 
AACGGTAACA TTGCCATCAT GTACCCATCG ATTTATGGGG ATACATTTAC CAAAACACCA 
AAGATGGATC CTGCCCAGCT TGATGTGTTT AAGACAGTTA AGGAAATTAC TGGATACTTG 
TGGATTCAGA CGTGGCCAGC AAGCATGAGC TCACTTAGTC CTTTTGAGAA TCTGGAGATC 
ATTCGAGGAA GAACTAAACG TGCGAGTCGC AGTTTGGTTA TGACTTATCT CGGTATCAAC 
TATCTAGGCC TTAAGTCTCT CAAAGAAATC ACCGATGGAG ACGTGAGCAT CTTTAAGAAC 
CGGGACCTCT GCTACACCAA CATCAGCCAC TGGAAGAGGT TCTTCAAATC AGACAGCCAA 
AGTGCTATGA TAGAAGGAAA CGCTGATGCT GCCACGTGCG CTCTGAGGAA CAACACGTGT 
GACAGGAAGT GTACCACAGA CGGCTGCTGG GGTCCAGGCC CTGACATGTG TTTTGCCTGT 
CGTGACTACA GCCGTGATGG GAGTTGTGTT GACTCCTGCA ACATCCTGGA GGGAGAACCA 
CGGGAGGCAG TCGTGAAAAA AACCTGCACT GAGTGTCACC CAGAGTGTCA GCGAATGAAT 
GGGACTGTGA CCTGCAGCGC GCCTGGTTCT GGAAACTGTG CTAAGTGTGC CAACTTCCAA 
GATGGACTGT TCTGTGTCTC CCGCTGTCCC CAAGGCATGC CAGGGGAGGA CGACATGCTA 
GTGTGGAAAT ATGCAGACGA GATGAAAGTG TGCCAGCTCT GCCACAAAAA CTGCACTCAA 
GGGTGCATTG GACCTGGCCT CGGCGGCTGC CATATTAAAA GCCTGCTGTC TTTCAGCCCC 
CCTGGTCTTT CCATGATTGC GGCTGGTGTA GTTGGTGGGC TGCTGGCTGT TCTTATTGCA 
GGCCTGTCTG TCTTTGTGTT GCTCCGCCGA CGCCACATCA AGAGGAAGAG AACCATGCGC 
AGACTGCTCC AAGAGAGAGA GTTGGTTGAA CCTCTTACTC CAAGCGGAGA GGCGCCAAAC 
CAGGCTCTGC TGCGGATCCT GAAGGAACCT GAATTTAAGA AAATCAAAGT GCTGGGATCC 
GGAGCGTTTG GTACAGTTTA CAAGGGTCTG TGGGTTCCAG AGGGAGAAGA TGTGAAGATC 
CCTGTGGCCA TCAAGGTTTT AAGAGAGGCC ACATCACCAA AAGCAAACAA GGAGATCTTG 
GACGAAGCCT ATGTGATGGC GAGTGTGGAG CACCCCCATG TGTGTCGTCT GCTGGGCATC 
TGCCTAACCT CCACAGTGCA GCTCATAACC CAGCTGATGC CCTACGGCTG CCTGCTGGAC 
TACGTCAAAG AGAACAAGGA CAATATCGGC TCCCAGCACC TGCTCAACTG GTGTGTGCAG 
ATCGCCAAGG GTATGAACTA CCTGGAGGAG CGCCACTTGG TGCATCGTGA CTTGGCAGCG 
AGAAACGTCC TGGTGAAGAC TCCTCAACAC GTCAAGATTA CAGACTTTGG TCTGGCCAAA 
CTCCTCAGTT CGGACGAGAA AGAGTACCAC GCAGACGGAG GAAAGGTACC GATTAAATGG 
ATGGCTCTTG AATCTATTCT GAACAGAACC TACACACACC AGAGTGATGT ATGGAGTTAC 
GGTGTAACAG TCTGGGAGCT GATGACATTT GGGACCAAAC CATATGATGG GATTCCAGCC 
AGTGAAATAG CCGGGGTCCT GGAGAAAGGA GAGCGCCTAC CTCAACCACC AATCTGTACT 
ATAGATGTCT ACATGATCAT GGTGAAATGT TGGATGATCG ATGCAGACAG CCGGCCTCGT 
TTCCGAGAAC TAATAGCTGA ATTTACAAAG ATGGCTCGGG ATCCATCCCG TTATCTTGTC 
ATCCAGGGTG ATGACCGCAT GCACCTACCC AGTCCCACAG ATACTAAATT CTACGGCAGC 
CTGATCAGCA GTGAGGACAT GGAGGATGCT GTGGATGCAG ATGAATACCT GGTACCACAG 
CATGGCTTCT TCAGCAGCCC GAGCACCTCC CATACCCCAC TGCTTCACTC CACAAGCCTT 
AACAGCAGCA TTGGAACATG CCACGGCAGA AATGGCTTTA TGAGCGGATA CCCGAGCAGA 
GATGGAAGCA TGGTCCTCCG ATACATTCCT GACCCCACAG ACAAAATTTT GGACAATGCC 
TTCCAGCCCG CTCCAGACTA CATGAACCAG AATGGAGTCT CAGATGTGGT GAATCCTAAC 
TACCAGCATC CAGGTCCACC TCGCGCCATT CTTCCCACCA TCTCTTCTGA CGATACAGAG 
ACTGAGTACT TGAACTGCTT TAAGAACGGG ACCAACGGAC CCGAATACCT CAATGAGGTC 
CCGACCCCCG CCATCCTCCC GCTCACCTCA AACGGCATAG TCCACAGCAT TCAGAAATAC 
CATCCCCAGA ATAGCATAGA CAACCCCGAT TACCAACAAG ATTTCACTCC CACCTTCAAA 
ACCCACGCTA ACGGACACAT ACCGGCAGCG GAGAACGCAG AGTACCTGGG CCCAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023133605.1
CDS	161..3760
Translation	MASVFLKWITSLLWLGSCVLAERKVCQGISNRFNLLGSKDNHYLNMVKTYGNCTVVLENLEVTHMEEHRDLSFLRSIEEVGGYVMIAINTASRISLENLRVIRGHTLYEGEFALVVFGNYNKESNMGTNQLHLNSLTEILKGGVKFGRNRLCNVETIQWYDIVSADNKPKMELPVASNNPLCRRCDSSCFNGSCWASGPQNCQTLTKLNCAQQCSKRCKGPSPSDCCNEHCAAGCTGPRPTDCLACRDFRDDGVCKDSCPGLMRYDPNLHQLVPNPHGKYNFGATCVKSCPHNYVVTDHGACVRTCSGNTYEVDEGGIRKCAKCDGLCPKVCNGLGTGDLTHAVSINATNIDSFKNCTKINGNIAIMYPSIYGDTFTKTPKMDPAQLDVFKTVKEITGYLWIQTWPASMSSLSPFENLEIIRGRTKRASRSLVMTYLGINYLGLKSLKEITDGDVSIFKNRDLCYTNISHWKRFFKSDSQSAMIEGNADAATCALRNNTCDRKCTTDGCWGPGPDMCFACRDYSRDGSCVDSCNILEGEPREAVVKKTCTECHPECQRMNGTVTCSAPGSGNCAKCANFQDGLFCVSRCPQGMPGEDDMLVWKYADEMKVCQLCHKNCTQGCIGPGLGGCHIKSLLSFSPPGLSMIAAGVVGGLLAVLIAGLSVFVLLRRRHIKRKRTMRRLLQERELVEPLTPSGEAPNQALLRILKEPEFKKIKVLGSGAFGTVYKGLWVPEGEDVKIPVAIKVLREATSPKANKEILDEAYVMASVEHPHVCRLLGICLTSTVQLITQLMPYGCLLDYVKENKDNIGSQHLLNWCVQIAKGMNYLEERHLVHRDLAARNVLVKTPQHVKITDFGLAKLLSSDEKEYHADGGKVPIKWMALESILNRTYTHQSDVWSYGVTVWELMTFGTKPYDGIPASEIAGVLEKGERLPQPPICTIDVYMIMVKCWMIDADSRPRFRELIAEFTKMARDPSRYLVIQGDDRMHLPSPTDTKFYGSLISSEDMEDAVDADEYLVPQHGFFSSPSTSHTPLLHSTSLNSSIGTCHGRNGFMSGYPSRDGSMVLRYIPDPTDKILDNAFQPAPDYMNQNGVSDVVNPNYQHPGPPRAILPTISSDDTETEYLNCFKNGTNGPEYLNEVPTPAILPLTSNGIVHSIQKYHPQNSIDNPDYQQDFTPTFKTHANGHIPAAENAEYLGPD

Target ORF information:

RefSeq Version	XM_023277837.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris epidermal growth factor receptor (egfr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023277837.1

ORF Insert Sequence:

ATGGCAAGCG TTTTTCTCAA GTGGATCACC TCTCTGCTCT GGCTGGGCTC CTGCGTTCTG 
GCGGAAAGGA AAGTATGCCA AGGCATCAGC AACCGGTTTA ACCTCCTGGG TTCGAAAGAC 
AACCACTACC TGAACATGGT GAAGACCTAC GGTAACTGCA CCGTGGTCCT GGAGAACCTG 
GAGGTCACCC ACATGGAGGA GCATCGTGAC CTGTCCTTCC TCAGGTCTAT TGAAGAAGTG 
GGTGGCTATG TCATGATTGC CATCAACACA GCATCCAGGA TTTCTCTGGA GAACCTGCGC 
GTCATCAGAG GACATACCCT TTATGAGGGA GAGTTTGCAC TTGTTGTGTT CGGCAATTAT 
AACAAAGAAT CAAATATGGG CACCAATCAG CTTCATCTAA ACAGTCTAAC AGAAATTCTA 
AAAGGAGGCG TGAAGTTTGG GAGAAACCGG CTGTGCAATG TAGAAACAAT ACAGTGGTAT 
GATATTGTCA GTGCCGACAA CAAACCCAAG ATGGAGCTCC CAGTTGCTAG CAACAACCCA 
CTTTGTAGAA GATGTGACTC GAGCTGCTTC AATGGTTCAT GTTGGGCATC TGGTCCTCAA 
AATTGTCAGA CTTTAACAAA GCTGAACTGT GCACAGCAGT GCTCTAAGAG ATGCAAAGGA 
CCTTCACCAA GTGACTGCTG CAATGAGCAT TGCGCCGCAG GATGCACAGG ACCCAGACCG 
ACCGACTGTC TGGCCTGTCG GGACTTCCGG GATGATGGGG TGTGTAAGGA CTCCTGCCCA 
GGCCTCATGC GCTACGATCC CAACCTGCAC CAGCTGGTTC CCAACCCACA TGGAAAGTAC 
AACTTCGGGG CCACCTGTGT CAAAAGCTGC CCACATAATT ATGTTGTGAC TGATCACGGA 
GCCTGTGTGC GGACGTGCAG CGGTAACACA TACGAGGTGG ATGAAGGAGG AATTAGGAAA 
TGCGCCAAGT GTGACGGACT GTGTCCAAAA GTGTGCAATG GACTTGGAAC AGGAGACCTG 
ACTCACGCTG TGTCCATTAA TGCCACAAAC ATCGACTCCT TTAAAAACTG CACCAAAATC 
AACGGTAACA TTGCCATCAT GTACCCATCG ATTTATGGGG ATACATTTAC CAAAACACCA 
AAGATGGATC CTGCCCAGCT TGATGTGTTT AAGACAGTTA AGGAAATTAC TGGATACTTG 
TGGATTCAGA CGTGGCCAGC AAGCATGAGC TCACTTAGTC CTTTTGAGAA TCTGGAGATC 
ATTCGAGGAA GAACTAAACG TGCGAGTCGC AGTTTGGTTA TGACTTATCT CGGTATCAAC 
TATCTAGGCC TTAAGTCTCT CAAAGAAATC ACCGATGGAG ACGTGAGCAT CTTTAAGAAC 
CGGGACCTCT GCTACACCAA CATCAGCCAC TGGAAGAGGT TCTTCAAATC AGACAGCCAA 
AGTGCTATGA TAGAAGGAAA CGCTGATGCT GCCACGTGCG CTCTGAGGAA CAACACGTGT 
GACAGGAAGT GTACCACAGA CGGCTGCTGG GGTCCAGGCC CTGACATGTG TTTTGCCTGT 
CGTGACTACA GCCGTGATGG GAGTTGTGTT GACTCCTGCA ACATCCTGGA GGGAGAACCA 
CGGGAGGCAG TCGTGAAAAA AACCTGCACT GAGTGTCACC CAGAGTGTCA GCGAATGAAT 
GGGACTGTGA CCTGCAGCGC GCCTGGTTCT GGAAACTGTG CTAAGTGTGC CAACTTCCAA 
GATGGACTGT TCTGTGTCTC CCGCTGTCCC CAAGGCATGC CAGGGGAGGA CGACATGCTA 
GTGTGGAAAT ATGCAGACGA GATGAAAGTG TGCCAGCTCT GCCACAAAAA CTGCACTCAA 
GGGTGCATTG GACCTGGCCT CGGCGGCTGC CATATTAAAA GCCTGCTGTC TTTCAGCCCC 
CCTGGTCTTT CCATGATTGC GGCTGGTGTA GTTGGTGGGC TGCTGGCTGT TCTTATTGCA 
GGCCTGTCTG TCTTTGTGTT GCTCCGCCGA CGCCACATCA AGAGGAAGAG AACCATGCGC 
AGACTGCTCC AAGAGAGAGA GTTGGTTGAA CCTCTTACTC CAAGCGGAGA GGCGCCAAAC 
CAGGCTCTGC TGCGGATCCT GAAGGAACCT GAATTTAAGA AAATCAAAGT GCTGGGATCC 
GGAGCGTTTG GTACAGTTTA CAAGGGTCTG TGGGTTCCAG AGGGAGAAGA TGTGAAGATC 
CCTGTGGCCA TCAAGGTTTT AAGAGAGGCC ACATCACCAA AAGCAAACAA GGAGATCTTG 
GACGAAGCCT ATGTGATGGC GAGTGTGGAG CACCCCCATG TGTGTCGTCT GCTGGGCATC 
TGCCTAACCT CCACAGTGCA GCTCATAACC CAGCTGATGC CCTACGGCTG CCTGCTGGAC 
TACGTCAAAG AGAACAAGGA CAATATCGGC TCCCAGCACC TGCTCAACTG GTGTGTGCAG 
ATCGCCAAGG GTATGAACTA CCTGGAGGAG CGCCACTTGG TGCATCGTGA CTTGGCAGCG 
AGAAACGTCC TGGTGAAGAC TCCTCAACAC GTCAAGATTA CAGACTTTGG TCTGGCCAAA 
CTCCTCAGTT CGGACGAGAA AGAGTACCAC GCAGACGGAG GAAAGGTACC GATTAAATGG 
ATGGCTCTTG AATCTATTCT GAACAGAACC TACACACACC AGAGTGATGT ATGGAGTTAC 
GGTGTAACAG TCTGGGAGCT GATGACATTT GGGACCAAAC CATATGATGG GATTCCAGCC 
AGTGAAATAG CCGGGGTCCT GGAGAAAGGA GAGCGCCTAC CTCAACCACC AATCTGTACT 
ATAGATGTCT ACATGATCAT GGTGAAATGT TGGATGATCG ATGCAGACAG CCGGCCTCGT 
TTCCGAGAAC TAATAGCTGA ATTTACAAAG ATGGCTCGGG ATCCATCCCG TTATCTTGTC 
ATCCAGGGTG ATGACCGCAT GCACCTACCC AGTCCCACAG ATACTAAATT CTACGGCAGC 
CTGATCAGCA GTGAGGACAT GGAGGATGCT GTGGATGCAG ATGAATACCT GGTACCACAG 
CATGGCTTCT TCAGCAGCCC GAGCACCTCC CATACCCCAC TGCTTCACTC CACAAGCCTT 
AACAGCAGCA TTGGAACATG CCACGGCAGA AATGGCTTTA TGAGCGGATA CCCGAGCAGA 
GATGGAAGCA TGGTCCTCCG ATACATTCCT GACCCCACAG ACAAAATTTT GGACAATGCC 
TTCCAGCCCG CTCCAGACTA CATGAACCAG AATGGAGTCT CAGATGTGGT GAATCCTAAC 
TACCAGCATC CAGGTCCACC TCGCGCCATT CTTCCCACCA TCTCTTCTGA CGATACAGAG 
ACTGAGTACT TGAACTGCTT TAAGAACGGG ACCAACGGAC CCGAATACCT CAATGAGGTC 
CCGACCCCCG CCATCCTCCC GCTCACCTCA AACGGCATAG TCCACAGCAT TCAGAAATAC 
CATCCCCAGA ATAGCATAGA CAACCCCGAT TACCAACAAG ATTTCACTCC CACCTTCAAA 
ACCCACGCTA ACGGACACAT ACCGGCAGCG GAGAACGCAG AGTACCTGGG CCCAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egfr ( XM_023277838.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023133606.1 Amphiprion ocellaris epidermal growth factor receptor (egfr), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAm83856
Clone ID Related Accession (Same CDS sequence)	XM_023277838.1
Accession Version	XM_023277838.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	epidermal growth factor receptor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019372753.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGCG TTTTTCTCAA GTGGATCACC TCTCTGCTCT GGCTGGGCTC CTGCGTTCTG 
GCGGAAAGGA AAGTATGCCA AGGCATCAGC AACCGGTTTA ACCTCCTGGG TTCGAAAGAC 
AACCACTACC TGAACATGGT GAAGACCTAC GGTAACTGCA CCGTGGTCCT GGAGAACCTG 
GAGGTCACCC ACATGGAGGA GCATCGTGAC CTGTCCTTCC TCAGGTCTAT TGAAGAAGTG 
GGTGGCTATG TCATGATTGC CATCAACACA GCATCCAGGA TTTCTCTGGA GAACCTGCGC 
GTCATCAGAG GACATACCCT TTATGAGGGA GAGTTTGCAC TTGTTGTGTT CGGCAATTAT 
AACAAAGAAT CAAATATGGG CACCAATCAG CTTCATCTAA ACAGTCTAAC AGAAATTCTA 
AAAGGAGGCG TGAAGTTTGG GAGAAACCGG CTGTGCAATG TAGAAACAAT ACAGTGGTAT 
GATATTGTCA GTGCCGACAA CAAACCCAAG ATGGAGCTCC CAGTTGCTAG CAACAACCCA 
CTTTGTAGAA GATGTGACTC GAGCTGCTTC AATGGTTCAT GTTGGGCATC TGGTCCTCAA 
AATTGTCAGA CTTTAACAAA GCTGAACTGT GCACAGCAGT GCTCTAAGAG ATGCAAAGGA 
CCTTCACCAA GTGACTGCTG CAATGAGCAT TGCGCCGCAG GATGCACAGG ACCCAGACCG 
ACCGACTGTC TGGCCTGTCG GGACTTCCGG GATGATGGGG TGTGTAAGGA CTCCTGCCCA 
GGCCTCATGC GCTACGATCC CAACCTGCAC CAGCTGGTTC CCAACCCACA TGGAAAGTAC 
AACTTCGGGG CCACCTGTGT CAAAAGCTGC CCACATAATT ATGTTGTGAC TGATCACGGA 
GCCTGTGTGC GGACGTGCAG CGGTAACACA TACGAGGTGG ATGAAGGAGG AATTAGGAAA 
TGCGCCAAGT GTGACGGACT GTGTCCAAAA GTGTGCAATG GACTTGGAAC AGGAGACCTG 
ACTCACGCTG TGTCCATTAA TGCCACAAAC ATCGACTCCT TTAAAAACTG CACCAAAATC 
AACGGTAACA TTGCCATCAT GTACCCATCG ATTTATGGGG ATACATTTAC CAAAACACCA 
AAGATGGATC CTGCCCAGCT TGATGTGTTT AAGACAGTTA AGGAAATTAC TGGATACTTG 
TGGATTCAGA CGTGGCCAGC AAGCATGAGC TCACTTAGTC CTTTTGAGAA TCTGGAGATC 
ATTCGAGGAA GAACTAAACG TGCGAGTCGC AGTTTGGTTA TGACTTATCT CGGTATCAAC 
TATCTAGGCC TTAAGTCTCT CAAAGAAATC ACCGATGGAG ACGTGAGCAT CTTTAAGAAC 
CGGGACCTCT GCTACACCAA CATCAGCCAC TGGAAGAGGT TCTTCAAATC AGACAGCCAA 
AGTGCTATGA TAGAAGGAAA CGCTGATGCT GCCACGTGCG CTCTGAGGAA CAACACGTGT 
GACAGGAAGT GTACCACAGA CGGCTGCTGG GGTCCAGGCC CTGACATGTG TTTTGCCTGT 
CGTGACTACA GCCGTGATGG GAGTTGTGTT GACTCCTGCA ACATCCTGGA GGGAGAACCA 
CGGGAGGCAG TCGTGAAAAA AACCTGCACT GAGTGTCACC CAGAGTGTCA GCGAATGAAT 
GGGACTGTGA CCTGCAGCGC GCCTGGTTCT GGAAACTGTG CTAAGTGTGC CAACTTCCAA 
GATGGACTGT TCTGTGTCTC CCGCTGTCCC CAAGGCATGC CAGGGGAGGA CGACATGCTA 
GTGTGGAAAT ATGCAGACGA GATGAAAGTG TGCCAGCTCT GCCACAAAAA CTGCACTCAA 
GGGTGCATTG GACCTGGCCT CGGCGGCTGC CATATTAAAA GCCCCCCTGG TCTTTCCATG 
ATTGCGGCTG GTGTAGTTGG TGGGCTGCTG GCTGTTCTTA TTGCAGGCCT GTCTGTCTTT 
GTGTTGCTCC GCCGACGCCA CATCAAGAGG AAGAGAACCA TGCGCAGACT GCTCCAAGAG 
AGAGAGTTGG TTGAACCTCT TACTCCAAGC GGAGAGGCGC CAAACCAGGC TCTGCTGCGG 
ATCCTGAAGG AACCTGAATT TAAGAAAATC AAAGTGCTGG GATCCGGAGC GTTTGGTACA 
GTTTACAAGG GTCTGTGGGT TCCAGAGGGA GAAGATGTGA AGATCCCTGT GGCCATCAAG 
GTTTTAAGAG AGGCCACATC ACCAAAAGCA AACAAGGAGA TCTTGGACGA AGCCTATGTG 
ATGGCGAGTG TGGAGCACCC CCATGTGTGT CGTCTGCTGG GCATCTGCCT AACCTCCACA 
GTGCAGCTCA TAACCCAGCT GATGCCCTAC GGCTGCCTGC TGGACTACGT CAAAGAGAAC 
AAGGACAATA TCGGCTCCCA GCACCTGCTC AACTGGTGTG TGCAGATCGC CAAGGGTATG 
AACTACCTGG AGGAGCGCCA CTTGGTGCAT CGTGACTTGG CAGCGAGAAA CGTCCTGGTG 
AAGACTCCTC AACACGTCAA GATTACAGAC TTTGGTCTGG CCAAACTCCT CAGTTCGGAC 
GAGAAAGAGT ACCACGCAGA CGGAGGAAAG GTACCGATTA AATGGATGGC TCTTGAATCT 
ATTCTGAACA GAACCTACAC ACACCAGAGT GATGTATGGA GTTACGGTGT AACAGTCTGG 
GAGCTGATGA CATTTGGGAC CAAACCATAT GATGGGATTC CAGCCAGTGA AATAGCCGGG 
GTCCTGGAGA AAGGAGAGCG CCTACCTCAA CCACCAATCT GTACTATAGA TGTCTACATG 
ATCATGGTGA AATGTTGGAT GATCGATGCA GACAGCCGGC CTCGTTTCCG AGAACTAATA 
GCTGAATTTA CAAAGATGGC TCGGGATCCA TCCCGTTATC TTGTCATCCA GGGTGATGAC 
CGCATGCACC TACCCAGTCC CACAGATACT AAATTCTACG GCAGCCTGAT CAGCAGTGAG 
GACATGGAGG ATGCTGTGGA TGCAGATGAA TACCTGGTAC CACAGCATGG CTTCTTCAGC 
AGCCCGAGCA CCTCCCATAC CCCACTGCTT CACTCCACAA GCCTTAACAG CAGCATTGGA 
ACATGCCACG GCAGAAATGG CTTTATGAGC GGATACCCGA GCAGAGATGG AAGCATGGTC 
CTCCGATACA TTCCTGACCC CACAGACAAA ATTTTGGACA ATGCCTTCCA GCCCGCTCCA 
GACTACATGA ACCAGAATGG AGTCTCAGAT GTGGTGAATC CTAACTACCA GCATCCAGGT 
CCACCTCGCG CCATTCTTCC CACCATCTCT TCTGACGATA CAGAGACTGA GTACTTGAAC 
TGCTTTAAGA ACGGGACCAA CGGACCCGAA TACCTCAATG AGGTCCCGAC CCCCGCCATC 
CTCCCGCTCA CCTCAAACGG CATAGTCCAC AGCATTCAGA AATACCATCC CCAGAATAGC 
ATAGACAACC CCGATTACCA ACAAGATTTC ACTCCCACCT TCAAAACCCA CGCTAACGGA 
CACATACCGG CAGCGGAGAA CGCAGAGTAC CTGGGCCCAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023133606.1
CDS	161..3745
Translation	MASVFLKWITSLLWLGSCVLAERKVCQGISNRFNLLGSKDNHYLNMVKTYGNCTVVLENLEVTHMEEHRDLSFLRSIEEVGGYVMIAINTASRISLENLRVIRGHTLYEGEFALVVFGNYNKESNMGTNQLHLNSLTEILKGGVKFGRNRLCNVETIQWYDIVSADNKPKMELPVASNNPLCRRCDSSCFNGSCWASGPQNCQTLTKLNCAQQCSKRCKGPSPSDCCNEHCAAGCTGPRPTDCLACRDFRDDGVCKDSCPGLMRYDPNLHQLVPNPHGKYNFGATCVKSCPHNYVVTDHGACVRTCSGNTYEVDEGGIRKCAKCDGLCPKVCNGLGTGDLTHAVSINATNIDSFKNCTKINGNIAIMYPSIYGDTFTKTPKMDPAQLDVFKTVKEITGYLWIQTWPASMSSLSPFENLEIIRGRTKRASRSLVMTYLGINYLGLKSLKEITDGDVSIFKNRDLCYTNISHWKRFFKSDSQSAMIEGNADAATCALRNNTCDRKCTTDGCWGPGPDMCFACRDYSRDGSCVDSCNILEGEPREAVVKKTCTECHPECQRMNGTVTCSAPGSGNCAKCANFQDGLFCVSRCPQGMPGEDDMLVWKYADEMKVCQLCHKNCTQGCIGPGLGGCHIKSPPGLSMIAAGVVGGLLAVLIAGLSVFVLLRRRHIKRKRTMRRLLQERELVEPLTPSGEAPNQALLRILKEPEFKKIKVLGSGAFGTVYKGLWVPEGEDVKIPVAIKVLREATSPKANKEILDEAYVMASVEHPHVCRLLGICLTSTVQLITQLMPYGCLLDYVKENKDNIGSQHLLNWCVQIAKGMNYLEERHLVHRDLAARNVLVKTPQHVKITDFGLAKLLSSDEKEYHADGGKVPIKWMALESILNRTYTHQSDVWSYGVTVWELMTFGTKPYDGIPASEIAGVLEKGERLPQPPICTIDVYMIMVKCWMIDADSRPRFRELIAEFTKMARDPSRYLVIQGDDRMHLPSPTDTKFYGSLISSEDMEDAVDADEYLVPQHGFFSSPSTSHTPLLHSTSLNSSIGTCHGRNGFMSGYPSRDGSMVLRYIPDPTDKILDNAFQPAPDYMNQNGVSDVVNPNYQHPGPPRAILPTISSDDTETEYLNCFKNGTNGPEYLNEVPTPAILPLTSNGIVHSIQKYHPQNSIDNPDYQQDFTPTFKTHANGHIPAAENAEYLGPD

Target ORF information:

RefSeq Version	XM_023277838.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris epidermal growth factor receptor (egfr), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023277838.1

ORF Insert Sequence:

ATGGCAAGCG TTTTTCTCAA GTGGATCACC TCTCTGCTCT GGCTGGGCTC CTGCGTTCTG 
GCGGAAAGGA AAGTATGCCA AGGCATCAGC AACCGGTTTA ACCTCCTGGG TTCGAAAGAC 
AACCACTACC TGAACATGGT GAAGACCTAC GGTAACTGCA CCGTGGTCCT GGAGAACCTG 
GAGGTCACCC ACATGGAGGA GCATCGTGAC CTGTCCTTCC TCAGGTCTAT TGAAGAAGTG 
GGTGGCTATG TCATGATTGC CATCAACACA GCATCCAGGA TTTCTCTGGA GAACCTGCGC 
GTCATCAGAG GACATACCCT TTATGAGGGA GAGTTTGCAC TTGTTGTGTT CGGCAATTAT 
AACAAAGAAT CAAATATGGG CACCAATCAG CTTCATCTAA ACAGTCTAAC AGAAATTCTA 
AAAGGAGGCG TGAAGTTTGG GAGAAACCGG CTGTGCAATG TAGAAACAAT ACAGTGGTAT 
GATATTGTCA GTGCCGACAA CAAACCCAAG ATGGAGCTCC CAGTTGCTAG CAACAACCCA 
CTTTGTAGAA GATGTGACTC GAGCTGCTTC AATGGTTCAT GTTGGGCATC TGGTCCTCAA 
AATTGTCAGA CTTTAACAAA GCTGAACTGT GCACAGCAGT GCTCTAAGAG ATGCAAAGGA 
CCTTCACCAA GTGACTGCTG CAATGAGCAT TGCGCCGCAG GATGCACAGG ACCCAGACCG 
ACCGACTGTC TGGCCTGTCG GGACTTCCGG GATGATGGGG TGTGTAAGGA CTCCTGCCCA 
GGCCTCATGC GCTACGATCC CAACCTGCAC CAGCTGGTTC CCAACCCACA TGGAAAGTAC 
AACTTCGGGG CCACCTGTGT CAAAAGCTGC CCACATAATT ATGTTGTGAC TGATCACGGA 
GCCTGTGTGC GGACGTGCAG CGGTAACACA TACGAGGTGG ATGAAGGAGG AATTAGGAAA 
TGCGCCAAGT GTGACGGACT GTGTCCAAAA GTGTGCAATG GACTTGGAAC AGGAGACCTG 
ACTCACGCTG TGTCCATTAA TGCCACAAAC ATCGACTCCT TTAAAAACTG CACCAAAATC 
AACGGTAACA TTGCCATCAT GTACCCATCG ATTTATGGGG ATACATTTAC CAAAACACCA 
AAGATGGATC CTGCCCAGCT TGATGTGTTT AAGACAGTTA AGGAAATTAC TGGATACTTG 
TGGATTCAGA CGTGGCCAGC AAGCATGAGC TCACTTAGTC CTTTTGAGAA TCTGGAGATC 
ATTCGAGGAA GAACTAAACG TGCGAGTCGC AGTTTGGTTA TGACTTATCT CGGTATCAAC 
TATCTAGGCC TTAAGTCTCT CAAAGAAATC ACCGATGGAG ACGTGAGCAT CTTTAAGAAC 
CGGGACCTCT GCTACACCAA CATCAGCCAC TGGAAGAGGT TCTTCAAATC AGACAGCCAA 
AGTGCTATGA TAGAAGGAAA CGCTGATGCT GCCACGTGCG CTCTGAGGAA CAACACGTGT 
GACAGGAAGT GTACCACAGA CGGCTGCTGG GGTCCAGGCC CTGACATGTG TTTTGCCTGT 
CGTGACTACA GCCGTGATGG GAGTTGTGTT GACTCCTGCA ACATCCTGGA GGGAGAACCA 
CGGGAGGCAG TCGTGAAAAA AACCTGCACT GAGTGTCACC CAGAGTGTCA GCGAATGAAT 
GGGACTGTGA CCTGCAGCGC GCCTGGTTCT GGAAACTGTG CTAAGTGTGC CAACTTCCAA 
GATGGACTGT TCTGTGTCTC CCGCTGTCCC CAAGGCATGC CAGGGGAGGA CGACATGCTA 
GTGTGGAAAT ATGCAGACGA GATGAAAGTG TGCCAGCTCT GCCACAAAAA CTGCACTCAA 
GGGTGCATTG GACCTGGCCT CGGCGGCTGC CATATTAAAA GCCCCCCTGG TCTTTCCATG 
ATTGCGGCTG GTGTAGTTGG TGGGCTGCTG GCTGTTCTTA TTGCAGGCCT GTCTGTCTTT 
GTGTTGCTCC GCCGACGCCA CATCAAGAGG AAGAGAACCA TGCGCAGACT GCTCCAAGAG 
AGAGAGTTGG TTGAACCTCT TACTCCAAGC GGAGAGGCGC CAAACCAGGC TCTGCTGCGG 
ATCCTGAAGG AACCTGAATT TAAGAAAATC AAAGTGCTGG GATCCGGAGC GTTTGGTACA 
GTTTACAAGG GTCTGTGGGT TCCAGAGGGA GAAGATGTGA AGATCCCTGT GGCCATCAAG 
GTTTTAAGAG AGGCCACATC ACCAAAAGCA AACAAGGAGA TCTTGGACGA AGCCTATGTG 
ATGGCGAGTG TGGAGCACCC CCATGTGTGT CGTCTGCTGG GCATCTGCCT AACCTCCACA 
GTGCAGCTCA TAACCCAGCT GATGCCCTAC GGCTGCCTGC TGGACTACGT CAAAGAGAAC 
AAGGACAATA TCGGCTCCCA GCACCTGCTC AACTGGTGTG TGCAGATCGC CAAGGGTATG 
AACTACCTGG AGGAGCGCCA CTTGGTGCAT CGTGACTTGG CAGCGAGAAA CGTCCTGGTG 
AAGACTCCTC AACACGTCAA GATTACAGAC TTTGGTCTGG CCAAACTCCT CAGTTCGGAC 
GAGAAAGAGT ACCACGCAGA CGGAGGAAAG GTACCGATTA AATGGATGGC TCTTGAATCT 
ATTCTGAACA GAACCTACAC ACACCAGAGT GATGTATGGA GTTACGGTGT AACAGTCTGG 
GAGCTGATGA CATTTGGGAC CAAACCATAT GATGGGATTC CAGCCAGTGA AATAGCCGGG 
GTCCTGGAGA AAGGAGAGCG CCTACCTCAA CCACCAATCT GTACTATAGA TGTCTACATG 
ATCATGGTGA AATGTTGGAT GATCGATGCA GACAGCCGGC CTCGTTTCCG AGAACTAATA 
GCTGAATTTA CAAAGATGGC TCGGGATCCA TCCCGTTATC TTGTCATCCA GGGTGATGAC 
CGCATGCACC TACCCAGTCC CACAGATACT AAATTCTACG GCAGCCTGAT CAGCAGTGAG 
GACATGGAGG ATGCTGTGGA TGCAGATGAA TACCTGGTAC CACAGCATGG CTTCTTCAGC 
AGCCCGAGCA CCTCCCATAC CCCACTGCTT CACTCCACAA GCCTTAACAG CAGCATTGGA 
ACATGCCACG GCAGAAATGG CTTTATGAGC GGATACCCGA GCAGAGATGG AAGCATGGTC 
CTCCGATACA TTCCTGACCC CACAGACAAA ATTTTGGACA ATGCCTTCCA GCCCGCTCCA 
GACTACATGA ACCAGAATGG AGTCTCAGAT GTGGTGAATC CTAACTACCA GCATCCAGGT 
CCACCTCGCG CCATTCTTCC CACCATCTCT TCTGACGATA CAGAGACTGA GTACTTGAAC 
TGCTTTAAGA ACGGGACCAA CGGACCCGAA TACCTCAATG AGGTCCCGAC CCCCGCCATC 
CTCCCGCTCA CCTCAAACGG CATAGTCCAC AGCATTCAGA AATACCATCC CCAGAATAGC 
ATAGACAACC CCGATTACCA ACAAGATTTC ACTCCCACCT TCAAAACCCA CGCTAACGGA 
CACATACCGG CAGCGGAGAA CGCAGAGTAC CTGGGCCCAG ACTGA