ASAH2 cDNA ORF clone, Alligator sinensis(Chinese alligator)

Gene
Clones
gRNAs

Summary

Gene Symbol	ASAH2
Entrez Gene ID	102387682
Full Name	N-acylsphingosine amidohydrolase 2
Synonyms	ASAH2B
Gene Type	protein-coding
Organism	Alligator sinensis(Chinese alligator)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006022943.1	XP_006023005.1	neutral ceramidase

Pathways from BioSystems
Gene Ontology

The following ASAH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASAH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAl17577	XM_006022943.1 Latest version!	Alligator sinensis N-acylsphingosine amidohydrolase 2 (ASAH2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ASAH2 ( XM_006022943.1 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_006023005.1 Alligator sinensis N-acylsphingosine amidohydrolase 2 (ASAH2), mRNA.

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CloneID	OAl17577
Clone ID Related Accession (Same CDS sequence)	XM_006022943.1
Accession Version	XM_006022943.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Alligator sinensis(Chinese alligator)
Product	neutral ceramidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005842055.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Alligator sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGA GAGACAAAAC CACGTTTTCT TGTTTGGAGG TGATACTTAT TGTTCTCTTG 
GTGTTGATGA CGGCAGTTAC AGTGACCCTC CTAGTTCTCC ACTTTGTCTC TGACAAGAGC 
ACATCTGAAA TAAACAATGT GTCAACATCC ACATCTAGCC CGGGTTACAA CTTCACATCA 
ACTCAACATC CTTCAACTGT TGGCTCAAAG AACTACTTAA TTGGGGTTGG CCGGGCTGAT 
TGCACTGGGC CTATTGCAGA AATCCCTTTG ATGGGTTATG CCAGTCCTGA CCAGCTGGCT 
GGTGGGATCC TGTCACGACT GTACAGTCGA GCTTTCATTG TGGCCGAGCC TGATAATTCC 
AAAAGAGTGG TGTTTGTTAG TGCTGACATA GGGATGGTAT CCCAAAGACT TAGATTAGAG 
GTACTCAGTC AACTGAAGAG TAAATATGGG GATCTATACA GACAAGACAA TGTTGTATTG 
AGTGGCACTC ACACCCATTC AGGGCCTGCT GGGTACTTCC AATTCACGCT TTTTTTGATA 
ACTAGCAAGG GGCTTATTGA GCCATCCCTT AAAGCCATCG TCAATGGCAT TGTCAAGAGC 
ATTGATATAG CACACCAGAA TATGAGGAAA GGAAGGATTT TTATCAATAA AGGCTTGGTA 
GAAAACAGCC AGATCAACAG AAGTCCTTAC TCTTACCTTC AGAACCCACC ACTTGAAATA 
CGCAGGTATT CATCAAATAC AGACAAAGAA ATGGTGATGC TGAAGATGGT TGATGTGAGT 
GGACAGGAGC TAGGCTTTAT CAGCTGGTTT GCTGTTCACC CAGTCAGCAT GAACAACACA 
AATCATCTTG TAAATAGCGA CAACATGGGG TACGCATCTT ACCTGTTTGA ACAAGAGAAG 
AATAAAGGAC TGCTTCCCGG AGAGGGTTCC TTTGTAGCAG CCTTTGCATC ATCTAACCTG 
GGAGATGTGT CACCTAATAC CAAAGGCCCA CACTGCATAA ACACAGGAGA GACTTGTGAT 
AACCCCCAAA GTTATTGCCC TGTAGGTGGG GCCAAAATGT GCATGGCTGT GGGTCCTGGC 
ACTGATATGT TCGACAGTAC AAAAATTATA GGGCAAAATA TCTATTTGAA AGCAAAGGAA 
CTGTATGCAA CAGCTTCCCA AGAGATAACA GGAAATCTAA GTTCAGCTCA CCAGTGGGTA 
AACATGAGTG ATGTCACCGT CCAGTTCAAT GCCACACACA CAGCTAAAAC ATGTAAACCG 
GCCTTGGGCT ACAGCTTTGC TGCAGGTACT ATTGATGGAG TAGGAGCTTT CAATTTCACA 
CAAGGTGCTG TAGAAGGAGA TCCATTCTGG GATGCTGTTC GTGATCAGCT GCTTGGAGTA 
CCATCCAATG AAACGAAAGA TTGCCACAAA CCTAAGCCAA TCCTTTTTAA TACAGGAGAG 
ATGTTGCTCC CTTATCCCTG GCACCCTGAA ATTGTTGATA TTCAGATCAT TACCATTGGG 
TCCGTGGCAA TTCTTGCTGT TCCAGGAGAG TTTACAACGA TGTCTGGGCG TAGACTGAGA 
GAAGCTGTTA AAAGTGAATT TGAATCCCAA GGGACACATG GAATGAATGT TATAATTGCA 
GGCTTGTGTA ATATTTATAC CCATTATATT GCAACCTATG AAGAATATCA GGTTCAACGA 
TACGAGGCAG CATCCACCAT TTATGGGCCA CACACACTAT CTGCTTATAT TCAGCTGTTC 
AGAAGGCTGG CAAGGGCTAT AGCTAAGCAT GAAGTTCAGG ATTTACCCAA AGGTCCAGAG 
CCACCATTTT TTAATGTAAC CAACTTGACA TTGTTACCCA CTGTTTTACC TGATGTAGCA 
CCACTGAATA AAACATTTGG AGATGTATTA CAGGAAGTAA AACAACATTA CCGAGTGGGA 
GAAGTTGTTG AAGTAACTTT TGTAAGTGCA AATCCCAGAA ATTGGGCAGA TGATATGACT 
GATCACACCT TCCTTACAGT AGAGAAATAT GAGAATGTTT CTAGAAGCTG GCGTGTAGTA 
CATAATGATG CCTCCTGGGA AACCAGGTTT TACTGGTCCA AAGGATTACG TGGTCACAGC 
AATGCTACAA TAGAATGGCA TATCCCAAAT ACTACTGAAC CAGGTATCTA TCAAATACAA 
TACTTTGGGT ACCGTAAGGT AAAACCACCT TTGAAGCCAA GTGTGTTTTA TCCATTCAAA 
GGCACATCTT CAGCATTTGA AATAATAAAA TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006023005.1
CDS	1..2256
Translation	MGKRDKTTFSCLEVILIVLLVLMTAVTVTLLVLHFVSDKSTSEINNVSTSTSSPGYNFTSTQHPSTVGSKNYLIGVGRADCTGPIAEIPLMGYASPDQLAGGILSRLYSRAFIVAEPDNSKRVVFVSADIGMVSQRLRLEVLSQLKSKYGDLYRQDNVVLSGTHTHSGPAGYFQFTLFLITSKGLIEPSLKAIVNGIVKSIDIAHQNMRKGRIFINKGLVENSQINRSPYSYLQNPPLEIRRYSSNTDKEMVMLKMVDVSGQELGFISWFAVHPVSMNNTNHLVNSDNMGYASYLFEQEKNKGLLPGEGSFVAAFASSNLGDVSPNTKGPHCINTGETCDNPQSYCPVGGAKMCMAVGPGTDMFDSTKIIGQNIYLKAKELYATASQEITGNLSSAHQWVNMSDVTVQFNATHTAKTCKPALGYSFAAGTIDGVGAFNFTQGAVEGDPFWDAVRDQLLGVPSNETKDCHKPKPILFNTGEMLLPYPWHPEIVDIQIITIGSVAILAVPGEFTTMSGRRLREAVKSEFESQGTHGMNVIIAGLCNIYTHYIATYEEYQVQRYEAASTIYGPHTLSAYIQLFRRLARAIAKHEVQDLPKGPEPPFFNVTNLTLLPTVLPDVAPLNKTFGDVLQEVKQHYRVGEVVEVTFVSANPRNWADDMTDHTFLTVEKYENVSRSWRVVHNDASWETRFYWSKGLRGHSNATIEWHIPNTTEPGIYQIQYFGYRKVKPPLKPSVFYPFKGTSSAFEIIKL

Target ORF information:

RefSeq Version	XM_006022943.1
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis N-acylsphingosine amidohydrolase 2 (ASAH2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006022943.1

ORF Insert Sequence:

ATGGGGAAGA GAGACAAAAC CACGTTTTCT TGTTTGGAGG TGATACTTAT TGTTCTCTTG 
GTGTTGATGA CGGCAGTTAC AGTGACCCTC CTAGTTCTCC ACTTTGTCTC TGACAAGAGC 
ACATCTGAAA TAAACAATGT GTCAACATCC ACATCTAGCC CGGGTTACAA CTTCACATCA 
ACTCAACATC CTTCAACTGT TGGCTCAAAG AACTACTTAA TTGGGGTTGG CCGGGCTGAT 
TGCACTGGGC CTATTGCAGA AATCCCTTTG ATGGGTTATG CCAGTCCTGA CCAGCTGGCT 
GGTGGGATCC TGTCACGACT GTACAGTCGA GCTTTCATTG TGGCCGAGCC TGATAATTCC 
AAAAGAGTGG TGTTTGTTAG TGCTGACATA GGGATGGTAT CCCAAAGACT TAGATTAGAG 
GTACTCAGTC AACTGAAGAG TAAATATGGG GATCTATACA GACAAGACAA TGTTGTATTG 
AGTGGCACTC ACACCCATTC AGGGCCTGCT GGGTACTTCC AATTCACGCT TTTTTTGATA 
ACTAGCAAGG GGCTTATTGA GCCATCCCTT AAAGCCATCG TCAATGGCAT TGTCAAGAGC 
ATTGATATAG CACACCAGAA TATGAGGAAA GGAAGGATTT TTATCAATAA AGGCTTGGTA 
GAAAACAGCC AGATCAACAG AAGTCCTTAC TCTTACCTTC AGAACCCACC ACTTGAAATA 
CGCAGGTATT CATCAAATAC AGACAAAGAA ATGGTGATGC TGAAGATGGT TGATGTGAGT 
GGACAGGAGC TAGGCTTTAT CAGCTGGTTT GCTGTTCACC CAGTCAGCAT GAACAACACA 
AATCATCTTG TAAATAGCGA CAACATGGGG TACGCATCTT ACCTGTTTGA ACAAGAGAAG 
AATAAAGGAC TGCTTCCCGG AGAGGGTTCC TTTGTAGCAG CCTTTGCATC ATCTAACCTG 
GGAGATGTGT CACCTAATAC CAAAGGCCCA CACTGCATAA ACACAGGAGA GACTTGTGAT 
AACCCCCAAA GTTATTGCCC TGTAGGTGGG GCCAAAATGT GCATGGCTGT GGGTCCTGGC 
ACTGATATGT TCGACAGTAC AAAAATTATA GGGCAAAATA TCTATTTGAA AGCAAAGGAA 
CTGTATGCAA CAGCTTCCCA AGAGATAACA GGAAATCTAA GTTCAGCTCA CCAGTGGGTA 
AACATGAGTG ATGTCACCGT CCAGTTCAAT GCCACACACA CAGCTAAAAC ATGTAAACCG 
GCCTTGGGCT ACAGCTTTGC TGCAGGTACT ATTGATGGAG TAGGAGCTTT CAATTTCACA 
CAAGGTGCTG TAGAAGGAGA TCCATTCTGG GATGCTGTTC GTGATCAGCT GCTTGGAGTA 
CCATCCAATG AAACGAAAGA TTGCCACAAA CCTAAGCCAA TCCTTTTTAA TACAGGAGAG 
ATGTTGCTCC CTTATCCCTG GCACCCTGAA ATTGTTGATA TTCAGATCAT TACCATTGGG 
TCCGTGGCAA TTCTTGCTGT TCCAGGAGAG TTTACAACGA TGTCTGGGCG TAGACTGAGA 
GAAGCTGTTA AAAGTGAATT TGAATCCCAA GGGACACATG GAATGAATGT TATAATTGCA 
GGCTTGTGTA ATATTTATAC CCATTATATT GCAACCTATG AAGAATATCA GGTTCAACGA 
TACGAGGCAG CATCCACCAT TTATGGGCCA CACACACTAT CTGCTTATAT TCAGCTGTTC 
AGAAGGCTGG CAAGGGCTAT AGCTAAGCAT GAAGTTCAGG ATTTACCCAA AGGTCCAGAG 
CCACCATTTT TTAATGTAAC CAACTTGACA TTGTTACCCA CTGTTTTACC TGATGTAGCA 
CCACTGAATA AAACATTTGG AGATGTATTA CAGGAAGTAA AACAACATTA CCGAGTGGGA 
GAAGTTGTTG AAGTAACTTT TGTAAGTGCA AATCCCAGAA ATTGGGCAGA TGATATGACT 
GATCACACCT TCCTTACAGT AGAGAAATAT GAGAATGTTT CTAGAAGCTG GCGTGTAGTA 
CATAATGATG CCTCCTGGGA AACCAGGTTT TACTGGTCCA AAGGATTACG TGGTCACAGC 
AATGCTACAA TAGAATGGCA TATCCCAAAT ACTACTGAAC CAGGTATCTA TCAAATACAA 
TACTTTGGGT ACCGTAAGGT AAAACCACCT TTGAAGCCAA GTGTGTTTTA TCCATTCAAA 
GGCACATCTT CAGCATTTGA AATAATAAAA TTGTAG