HARS cDNA ORF clone, Alligator mississippiensis(American alligator)

Gene
Clones
gRNAs

Summary

Gene Symbol	HARS
Entrez Gene ID	102575240
Full Name	histidyl-tRNA synthetase
Gene Type	protein-coding
Organism	Alligator mississippiensis(American alligator)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006273416.3	XP_006273478.1	histidine--tRNA ligase, cytoplasmic

Pathways from BioSystems
Gene Ontology

The following HARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAl56190	XM_006273416.3 Latest version!	Alligator mississippiensis histidyl-tRNA synthetase (HARS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HARS ( XM_006273416.3 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_006273478.1 Alligator mississippiensis histidyl-tRNA synthetase (HARS), mRNA.

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CloneID	OAl56190
Clone ID Related Accession (Same CDS sequence)	XM_006273416.3
Accession Version	XM_006273416.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	histidine--tRNA ligase, cytoplasmic
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017709751.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 9, 2016 this sequence version replaced XM_006273416.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG AGGCAGCGGA GTTGGCTGAG GCGCTGCGCC TGCAGGCGGA CACGGTGCGG 
CGACTGAAGC AGGAGCGGGC CGGCACCGAG GAGGTGGCGG AGCAGGTGGC CAAGTTGCTG 
GAGCTGAAGG CACGGCTGGG CGTGGATGAC GGACGGCCCC GCTTCCTGCT CAAGACCCCC 
AAGGGCACCC GGGACTATGG CCCCAAGCAG ATGGCCATCC GGGAGAGAGT CTTCAGCACC 
ATCATCAGCT GTTTCAAGCG TCATGGGGCT GAGGTGATTG ACACCCCTGT GTTTGAGCTA 
AAGGAAACCC TGACTGGGAA GTATGGGGAG GACTCAAAGC TCATCTATGA CCTGAAGGAT 
CAGGGGGGAG AGCTGCTGTC TCTTCGCTAC GATCTGACTG TGCCTTTTGC TCGTTATTTG 
GCCATGAATA AAATCACGAA CATTAAACGC TACCACATTG CTAAAGTTTA CAGACGGGAT 
AACCCAGCCA TGACAAGGGG CCGCTACCGG GAGTTCTACC AGTGTGATTT TGATATTGCT 
GGCCAGTTTG ACCCCATGAT TCCTGATGCT GAGTGTCTGA AGATAGTGTA TGAGATCCTG 
TCTGCACTCC AGCTTGGGGA CTTCCTTGTT AAGGTCAATG ACCGGCGCAT TCTAGATGGG 
ATGTTTGCAG TTTGTGGAGT TCCAGACAAC AAGTTCCGGA CCATTTGCTC CAGTGTGGAC 
AAACTAGACA AAGTGATGTG GGAGGATGTG AGGAGCGAGA TAGTTGGAGA GAAGGGGCTC 
TCAACTGAGG CTGCAGATTG CATTGGGGAA TACGTTAGAC TCCACGGTGG GCTGGACCTG 
GTTGAAGAGC TGCTCCAGGA CCCAAAACTC TCCCAGAACA AGCTGGCCAG GGAGGGGCTG 
GCAGACATGA AGCTGCTGTT TGAGTACCTG ATGCTATTTG GCATTGCAGA TAAGATCTCC 
TTTGACCTGA GCCTGGCCCG GGGTCTGGAT TATTACACGG GGGTGATCTA CGAGGCTGTC 
CTGCTCCAGC AACAGAATGA CCATGTGGAG GAGCCAGCCA CTGTTGGCAG TGTGGCTGGA 
GGAGGCCGCT ATGATGGGCT GGTGGGGATG TTTGATCCCA AGGGGAAGAA GGTTCCCTGT 
GTGGGCGTTA GCATTGGAAT CGAACGGATC TTCTCCCTCA TGGAGCAGAA AATGGAGGCT 
TCCGAGGAGA AGATCCGGAC AACAGACACA CAAGTGCTTG TGGCATCTGC TCAAAAGAAA 
CTCCTTGAAG AGAGATTGAA GCTCATCTCT GAGCTGTGGG ATTCTGGGAT CAAGGCAGAA 
ATGCTATACA AGAAGAACCC CAAACTGCTG AACCAGCTGC AGTACTGCGA GGACACTGGG 
ATCCCTCTTG TTGCCATCAT AGGTGAGCAG GAGCTCAAGG ATGGAGTTGT CAAGCTGAGA 
GATGTAGCTA CCAGGGAGGA GGTTGATATT CGTAGGGAAA ATCTTGTTGA AGAAATCAAG 
AAGCGAACAT GCCAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006273478.1
CDS	44..1564
Translation	MAAEAAELAEALRLQADTVRRLKQERAGTEEVAEQVAKLLELKARLGVDDGRPRFLLKTPKGTRDYGPKQMAIRERVFSTIISCFKRHGAEVIDTPVFELKETLTGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKITNIKRYHIAKVYRRDNPAMTRGRYREFYQCDFDIAGQFDPMIPDAECLKIVYEILSALQLGDFLVKVNDRRILDGMFAVCGVPDNKFRTICSSVDKLDKVMWEDVRSEIVGEKGLSTEAADCIGEYVRLHGGLDLVEELLQDPKLSQNKLAREGLADMKLLFEYLMLFGIADKISFDLSLARGLDYYTGVIYEAVLLQQQNDHVEEPATVGSVAGGGRYDGLVGMFDPKGKKVPCVGVSIGIERIFSLMEQKMEASEEKIRTTDTQVLVASAQKKLLEERLKLISELWDSGIKAEMLYKKNPKLLNQLQYCEDTGIPLVAIIGEQELKDGVVKLRDVATREEVDIRRENLVEEIKKRTCQP

Target ORF information:

RefSeq Version	XM_006273416.3
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis histidyl-tRNA synthetase (HARS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006273416.3

ORF Insert Sequence:

ATGGCGGCTG AGGCAGCGGA GTTGGCTGAG GCGCTGCGCC TGCAGGCGGA CACGGTGCGG 
CGACTGAAGC AGGAGCGGGC CGGCACCGAG GAGGTGGCGG AGCAGGTGGC CAAGTTGCTG 
GAGCTGAAGG CACGGCTGGG CGTGGATGAC GGACGGCCCC GCTTCCTGCT CAAGACCCCC 
AAGGGCACCC GGGACTATGG CCCCAAGCAG ATGGCCATCC GGGAGAGAGT CTTCAGCACC 
ATCATCAGCT GTTTCAAGCG TCATGGGGCT GAGGTGATTG ACACCCCTGT GTTTGAGCTA 
AAGGAAACCC TGACTGGGAA GTATGGGGAG GACTCAAAGC TCATCTATGA CCTGAAGGAT 
CAGGGGGGAG AGCTGCTGTC TCTTCGCTAC GATCTGACTG TGCCTTTTGC TCGTTATTTG 
GCCATGAATA AAATCACGAA CATTAAACGC TACCACATTG CTAAAGTTTA CAGACGGGAT 
AACCCAGCCA TGACAAGGGG CCGCTACCGG GAGTTCTACC AGTGTGATTT TGATATTGCT 
GGCCAGTTTG ACCCCATGAT TCCTGATGCT GAGTGTCTGA AGATAGTGTA TGAGATCCTG 
TCTGCACTCC AGCTTGGGGA CTTCCTTGTT AAGGTCAATG ACCGGCGCAT TCTAGATGGG 
ATGTTTGCAG TTTGTGGAGT TCCAGACAAC AAGTTCCGGA CCATTTGCTC CAGTGTGGAC 
AAACTAGACA AAGTGATGTG GGAGGATGTG AGGAGCGAGA TAGTTGGAGA GAAGGGGCTC 
TCAACTGAGG CTGCAGATTG CATTGGGGAA TACGTTAGAC TCCACGGTGG GCTGGACCTG 
GTTGAAGAGC TGCTCCAGGA CCCAAAACTC TCCCAGAACA AGCTGGCCAG GGAGGGGCTG 
GCAGACATGA AGCTGCTGTT TGAGTACCTG ATGCTATTTG GCATTGCAGA TAAGATCTCC 
TTTGACCTGA GCCTGGCCCG GGGTCTGGAT TATTACACGG GGGTGATCTA CGAGGCTGTC 
CTGCTCCAGC AACAGAATGA CCATGTGGAG GAGCCAGCCA CTGTTGGCAG TGTGGCTGGA 
GGAGGCCGCT ATGATGGGCT GGTGGGGATG TTTGATCCCA AGGGGAAGAA GGTTCCCTGT 
GTGGGCGTTA GCATTGGAAT CGAACGGATC TTCTCCCTCA TGGAGCAGAA AATGGAGGCT 
TCCGAGGAGA AGATCCGGAC AACAGACACA CAAGTGCTTG TGGCATCTGC TCAAAAGAAA 
CTCCTTGAAG AGAGATTGAA GCTCATCTCT GAGCTGTGGG ATTCTGGGAT CAAGGCAGAA 
ATGCTATACA AGAAGAACCC CAAACTGCTG AACCAGCTGC AGTACTGCGA GGACACTGGG 
ATCCCTCTTG TTGCCATCAT AGGTGAGCAG GAGCTCAAGG ATGGAGTTGT CAAGCTGAGA 
GATGTAGCTA CCAGGGAGGA GGTTGATATT CGTAGGGAAA ATCTTGTTGA AGAAATCAAG 
AAGCGAACAT GCCAGCCCTG A