TKFC cDNA ORF clone, Alligator mississippiensis(American alligator)

Gene
Clones
gRNAs

Summary

Gene Symbol	TKFC
Entrez Gene ID	102566051
Full Name	triokinase and FMN cyclase
Synonyms	DAK
Gene Type	protein-coding
Organism	Alligator mississippiensis(American alligator)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019485271.1	XP_019340816.1	triokinase/FMN cyclase isoform X2
XM_014593468.2	XP_014448954.1	triokinase/FMN cyclase isoform X1

Pathways from BioSystems
Gene Ontology

The following TKFC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TKFC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAl53985	XM_014593468.2 Latest version!	Alligator mississippiensis triokinase and FMN cyclase (TKFC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	Quote Price
OAl53986	XM_019485271.1 Latest version!	Alligator mississippiensis triokinase and FMN cyclase (TKFC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TKFC ( XM_014593468.2 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_014448954.1 Alligator mississippiensis triokinase and FMN cyclase (TKFC), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAl53985
Clone ID Related Accession (Same CDS sequence)	XM_014593468.2
Accession Version	XM_014593468.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	triokinase/FMN cyclase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017709042.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 9, 2016 this sequence version replaced XM_014593468.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGTCT CCAAGAAACT GGTGAACTCT GTGCCAGGCT GTGCGGACGA TGCGCTGGCA 
GGGCTGGTGG CCTGTAACCC AGCCATACAG CTCCTGCAGG GGCATCGGGT GGCACTGCGA 
TCTGACCTGG AGAATGTCCG AGGCCGAGTG GCACTGCTTT CTGGAGGGGG GTCAGGACAT 
GAGCCCGCAC ATGCAGGGTA CATCGGGAAG GGAATGCTCA CTGGTGTGGT GGCTGGTGCG 
GTCTTCACCT CCCCTGCAGT GGGCAGCATA TTGGCAGCAA TCCGGGCAGT GACGCAGGCT 
GGTTCAGCCG GGACCCTGCT GATTGTGAAG AACTACACCG GAGACCGGCT GAATTTTGGG 
CTGGCCCTGG AGCAGGCACG GGCGGAGGGA GCTGATGTGC AGATGGTGGT GATTGGTGAT 
GACAGTGCTT TCACCACACT GAAGAAGGCT GGGAGGAGAG GCCTGTGTGG CACTGTGCTC 
ATCCACAAGG TGGCTGGGGC ATTGGCAGAG GCAGGTGCCA GCTTGGATGA GATCGTCAGC 
AGGGTGACAG CTGCTGCGAG AGCCATGGGC ACTTTGGGCG TGAGCCTGTC CCCCTGCAGT 
GTTCCTGGTT CCCAGCCTAC CTTTCAACTG GCACCTGATG AGCTGGAGCT GGGCCTGGGG 
ATTCATGGTG AGGCGGGTGT GCGCAGGATG AAGGTGGCAT CGGCTGATGA GATTGTGGAG 
ATAATGCTTA ATCACATGAC AAACCCTTCC AACGCTTCCC ATGTGCCTTT GGACTCTGGT 
GTCTCTGTGG TCCTTGTGGT AAATAACCTG GGTGGCCTGT CCTGTCTGGA GCTAGGTGTG 
GTGGCCGGCT CAGCTGTGCA CTGTCTGGAA AGCAGAGGTG TTCGCATTGC CCGAGCCCTG 
GTTGGCTCCT TCATGACTGC ACTGGAGATG GCTGGCGTCT CCCTCACCCT ACTGCTGGTG 
GATGACAAGC TCTTGAGACT GATTGATGCG GAGACCACTG CTGTAGCCTG GCCCAACCTA 
GCCAAAGCAT CTGTGACAGG TCGGAGCCGA GAGCTGGCAG CACCCAAGGG AGGATCTGAG 
TCAGAGGAGC CTGCCTCAGG CACCGCACCA TCCTGCCAGC ATACACACCT GGTCCTGGAG 
CGGGTGTGTG ACGTCCTTCT GGGCATGCAG GAGAAACTCA ATGAGCTGGA CCGCGCAGCA 
GGGGATGGGG ACTGCGGGAA CACACATGCC AGAGCTGCCA TAGCTATACA GGAATGGTTG 
AATGCAAGGC CTCCACCTGC CCAACCATCC CAGCTCCTCT CTGTCCTGGC CAGGCTGCTG 
CTGGAGAAGA TGGGGGGCTC CTCAGGGGTG CTGTATGGGC TGTTCCTTAC AGCAGCTGCA 
CGGCCACTGC AGAATTGCAG CAACCTTCCA GCCTGGGCTG ATGCCATGGA CACAGGCATT 
GAAGCCATGC AGCAGTATGG AGGAGCTGCG CCTGGGGACA GGACCATGCT GGATTCACTA 
TGTGCTGCTG CAGGGGAGCT GCATGCTTTG CGAGCCCCTG GTGCCGACCT GTTCCAGGTG 
CTGGCCAAGG CTGTCCAGAG TGCAGAGGCT GCAGCAGAAG CCACTAAGAA TATGGAGGCA 
GGTGCCGGCA GGGCCAGTTA CATCAGCTCA GCCAAGCTAC TGCAGCCGGA CCCTGGGGCT 
GTAGCAGTTT CAGCTGTGCT CCGTGCTGTG TTGGAGGGGC TGCAGAGCCA GAGTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014448954.1
CDS	102..1841
Translation	MQVSKKLVNSVPGCADDALAGLVACNPAIQLLQGHRVALRSDLENVRGRVALLSGGGSGHEPAHAGYIGKGMLTGVVAGAVFTSPAVGSILAAIRAVTQAGSAGTLLIVKNYTGDRLNFGLALEQARAEGADVQMVVIGDDSAFTTLKKAGRRGLCGTVLIHKVAGALAEAGASLDEIVSRVTAAARAMGTLGVSLSPCSVPGSQPTFQLAPDELELGLGIHGEAGVRRMKVASADEIVEIMLNHMTNPSNASHVPLDSGVSVVLVVNNLGGLSCLELGVVAGSAVHCLESRGVRIARALVGSFMTALEMAGVSLTLLLVDDKLLRLIDAETTAVAWPNLAKASVTGRSRELAAPKGGSESEEPASGTAPSCQHTHLVLERVCDVLLGMQEKLNELDRAAGDGDCGNTHARAAIAIQEWLNARPPPAQPSQLLSVLARLLLEKMGGSSGVLYGLFLTAAARPLQNCSNLPAWADAMDTGIEAMQQYGGAAPGDRTMLDSLCAAAGELHALRAPGADLFQVLAKAVQSAEAAAEATKNMEAGAGRASYISSAKLLQPDPGAVAVSAVLRAVLEGLQSQSV

Target ORF information:

RefSeq Version	XM_014593468.2
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis triokinase and FMN cyclase (TKFC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014593468.2

ORF Insert Sequence:

ATGCAGGTCT CCAAGAAACT GGTGAACTCT GTGCCAGGCT GTGCGGACGA TGCGCTGGCA 
GGGCTGGTGG CCTGTAACCC AGCCATACAG CTCCTGCAGG GGCATCGGGT GGCACTGCGA 
TCTGACCTGG AGAATGTCCG AGGCCGAGTG GCACTGCTTT CTGGAGGGGG GTCAGGACAT 
GAGCCCGCAC ATGCAGGGTA CATCGGGAAG GGAATGCTCA CTGGTGTGGT GGCTGGTGCG 
GTCTTCACCT CCCCTGCAGT GGGCAGCATA TTGGCAGCAA TCCGGGCAGT GACGCAGGCT 
GGTTCAGCCG GGACCCTGCT GATTGTGAAG AACTACACCG GAGACCGGCT GAATTTTGGG 
CTGGCCCTGG AGCAGGCACG GGCGGAGGGA GCTGATGTGC AGATGGTGGT GATTGGTGAT 
GACAGTGCTT TCACCACACT GAAGAAGGCT GGGAGGAGAG GCCTGTGTGG CACTGTGCTC 
ATCCACAAGG TGGCTGGGGC ATTGGCAGAG GCAGGTGCCA GCTTGGATGA GATCGTCAGC 
AGGGTGACAG CTGCTGCGAG AGCCATGGGC ACTTTGGGCG TGAGCCTGTC CCCCTGCAGT 
GTTCCTGGTT CCCAGCCTAC CTTTCAACTG GCACCTGATG AGCTGGAGCT GGGCCTGGGG 
ATTCATGGTG AGGCGGGTGT GCGCAGGATG AAGGTGGCAT CGGCTGATGA GATTGTGGAG 
ATAATGCTTA ATCACATGAC AAACCCTTCC AACGCTTCCC ATGTGCCTTT GGACTCTGGT 
GTCTCTGTGG TCCTTGTGGT AAATAACCTG GGTGGCCTGT CCTGTCTGGA GCTAGGTGTG 
GTGGCCGGCT CAGCTGTGCA CTGTCTGGAA AGCAGAGGTG TTCGCATTGC CCGAGCCCTG 
GTTGGCTCCT TCATGACTGC ACTGGAGATG GCTGGCGTCT CCCTCACCCT ACTGCTGGTG 
GATGACAAGC TCTTGAGACT GATTGATGCG GAGACCACTG CTGTAGCCTG GCCCAACCTA 
GCCAAAGCAT CTGTGACAGG TCGGAGCCGA GAGCTGGCAG CACCCAAGGG AGGATCTGAG 
TCAGAGGAGC CTGCCTCAGG CACCGCACCA TCCTGCCAGC ATACACACCT GGTCCTGGAG 
CGGGTGTGTG ACGTCCTTCT GGGCATGCAG GAGAAACTCA ATGAGCTGGA CCGCGCAGCA 
GGGGATGGGG ACTGCGGGAA CACACATGCC AGAGCTGCCA TAGCTATACA GGAATGGTTG 
AATGCAAGGC CTCCACCTGC CCAACCATCC CAGCTCCTCT CTGTCCTGGC CAGGCTGCTG 
CTGGAGAAGA TGGGGGGCTC CTCAGGGGTG CTGTATGGGC TGTTCCTTAC AGCAGCTGCA 
CGGCCACTGC AGAATTGCAG CAACCTTCCA GCCTGGGCTG ATGCCATGGA CACAGGCATT 
GAAGCCATGC AGCAGTATGG AGGAGCTGCG CCTGGGGACA GGACCATGCT GGATTCACTA 
TGTGCTGCTG CAGGGGAGCT GCATGCTTTG CGAGCCCCTG GTGCCGACCT GTTCCAGGTG 
CTGGCCAAGG CTGTCCAGAG TGCAGAGGCT GCAGCAGAAG CCACTAAGAA TATGGAGGCA 
GGTGCCGGCA GGGCCAGTTA CATCAGCTCA GCCAAGCTAC TGCAGCCGGA CCCTGGGGCT 
GTAGCAGTTT CAGCTGTGCT CCGTGCTGTG TTGGAGGGGC TGCAGAGCCA GAGTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TKFC ( XM_019485271.1 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_019340816.1 Alligator mississippiensis triokinase and FMN cyclase (TKFC), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAl53986
Clone ID Related Accession (Same CDS sequence)	XM_019485271.1
Accession Version	XM_019485271.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	triokinase/FMN cyclase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017709042.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGTCT CCAAGAAACT GGTGAACTCT GTGCCAGGCT GTGCGGACGA TGCGCTGGCA 
GGGCTGGTGG CCTGTAACCC AGCCATACAG CTCCTGCAGG GGCATCGGGT GGCACTGCGA 
TCTGACCTGG AGAATGTCCG AGGCCGAGTG GCACTGCTTT CTGGAGGGGG GTCAGGACAT 
GAGCCCGCAC ATGCAGGGTA CATCGGGAAG GGAATGCTCA CTGGTGTGGT GGCTGGTGCG 
GTCTTCACCT CCCCTGCAGT GGGCAGCATA TTGGCAGCAA TCCGGGCAGT GACGCAGGCT 
GGTTCAGCCG GGACCCTGCT GATTGTGAAG AACTACACCG GAGACCGGCT GAATTTTGGG 
CTGGCCCTGG AGCAGGCACG GGCGGAGGGA GCTGATGTGC AGATGGTGGT GATTGGTGAT 
GACAGTGCTT TCACCACACT GAAGAAGGCT GGGAGGAGAG GCCTGTGTGG CACTGTGCTC 
ATCCACAAGG TGGCTGGGGC ATTGGCAGAG GCAGGTGCCA GCTTGGATGA GATCGTCAGC 
AGGGTGACAG CTGCTGCGAG AGCCATGGGC ACTTTGGGCG TGAGCCTGTC CCCCTGCAGT 
GTTCCTGGTT CCCAGCCTAC CTTTCAACTG GCACCTGATG AGCTGGAGCT GGGCCTGGGG 
ATTCATGGTG AGGCGGGTGT GCGCAGGATG AAGGTGGCAT CGGCTGATGA GATTGTGGAG 
ATAATGCTTA ATCACATGAC AAACCCTTCC AACGCTTCCC ATGTGCCTTT GGACTCTGGT 
GTCTCTGTGG TCCTTGTGGT AAATAACCTG GGTGGCCTGT CCTGTCTGGA GCTAGGTGTG 
GTGGCCGGCT CAGCTGTGCA CTGTCTGGAA AGCAGAGGTG TTCGCATTGC CCGAGCCCTG 
GTTGGCTCCT TCATGACTGC ACTGGAGATG GCTGGCGTCT CCCTCACCCT ACTGCTGGTG 
GATGACAAGC TCTTGAGACT GATTGATGCG GAGACCACTG CTGTAGCCTG GCCCAACCTA 
GCCAAAGCAT CTGTGACAGG TCGGAGCCGA GAGCTGGCAG CACCCAAGGG AGGATCTGAG 
TCAGAGGAGC CTGCCTCAGG CACCGCACCA TCCTGCCAGC ATACACACCT GGTCCTGGAG 
CGGGTGTGTG ACGTCCTTCT GGGCATGCAG GAGAAACTCA ATGAGCTGGA CCGCGCAGCA 
GGGGATGGGG ACTGCGGGAA CACACATGCC AGAGCTGCCA TAGCTATACA GGAATGGTTG 
AATGCAAGGC CTCCACCTGC CCAACCATCC CAGCTCCTCT CTGTCCTGGC CAGGCTGCTG 
CTGGAGAAGA TGGGGGGCTC CTCAGGGGTG CTGTATGGGC TGTTCCTTAC AGCAGCTGCA 
CGGCCACTGC AGAATTGCAG CAACCTTCCA GCCTGGGCTG ATGCCATGGA CACAGGCATT 
GAAGCCATGC AGCAGTATGG AGGAGCTGCG CCTGGGGACA GGACCATGCT GGATTCACTA 
TGTGCTGCTG CAGGGGAGCT GCATGCTTTG CGAGCCCCTG GTGCCGACCT GTTCCAGGTG 
CTGGCCAAGG CTGTCCAGAC ACGCTGCAGA GGAGGACCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019340816.1
CDS	102..1703
Translation	MQVSKKLVNSVPGCADDALAGLVACNPAIQLLQGHRVALRSDLENVRGRVALLSGGGSGHEPAHAGYIGKGMLTGVVAGAVFTSPAVGSILAAIRAVTQAGSAGTLLIVKNYTGDRLNFGLALEQARAEGADVQMVVIGDDSAFTTLKKAGRRGLCGTVLIHKVAGALAEAGASLDEIVSRVTAAARAMGTLGVSLSPCSVPGSQPTFQLAPDELELGLGIHGEAGVRRMKVASADEIVEIMLNHMTNPSNASHVPLDSGVSVVLVVNNLGGLSCLELGVVAGSAVHCLESRGVRIARALVGSFMTALEMAGVSLTLLLVDDKLLRLIDAETTAVAWPNLAKASVTGRSRELAAPKGGSESEEPASGTAPSCQHTHLVLERVCDVLLGMQEKLNELDRAAGDGDCGNTHARAAIAIQEWLNARPPPAQPSQLLSVLARLLLEKMGGSSGVLYGLFLTAAARPLQNCSNLPAWADAMDTGIEAMQQYGGAAPGDRTMLDSLCAAAGELHALRAPGADLFQVLAKAVQTRCRGGP

Target ORF information:

RefSeq Version	XM_019485271.1
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis triokinase and FMN cyclase (TKFC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019485271.1

ORF Insert Sequence:

ATGCAGGTCT CCAAGAAACT GGTGAACTCT GTGCCAGGCT GTGCGGACGA TGCGCTGGCA 
GGGCTGGTGG CCTGTAACCC AGCCATACAG CTCCTGCAGG GGCATCGGGT GGCACTGCGA 
TCTGACCTGG AGAATGTCCG AGGCCGAGTG GCACTGCTTT CTGGAGGGGG GTCAGGACAT 
GAGCCCGCAC ATGCAGGGTA CATCGGGAAG GGAATGCTCA CTGGTGTGGT GGCTGGTGCG 
GTCTTCACCT CCCCTGCAGT GGGCAGCATA TTGGCAGCAA TCCGGGCAGT GACGCAGGCT 
GGTTCAGCCG GGACCCTGCT GATTGTGAAG AACTACACCG GAGACCGGCT GAATTTTGGG 
CTGGCCCTGG AGCAGGCACG GGCGGAGGGA GCTGATGTGC AGATGGTGGT GATTGGTGAT 
GACAGTGCTT TCACCACACT GAAGAAGGCT GGGAGGAGAG GCCTGTGTGG CACTGTGCTC 
ATCCACAAGG TGGCTGGGGC ATTGGCAGAG GCAGGTGCCA GCTTGGATGA GATCGTCAGC 
AGGGTGACAG CTGCTGCGAG AGCCATGGGC ACTTTGGGCG TGAGCCTGTC CCCCTGCAGT 
GTTCCTGGTT CCCAGCCTAC CTTTCAACTG GCACCTGATG AGCTGGAGCT GGGCCTGGGG 
ATTCATGGTG AGGCGGGTGT GCGCAGGATG AAGGTGGCAT CGGCTGATGA GATTGTGGAG 
ATAATGCTTA ATCACATGAC AAACCCTTCC AACGCTTCCC ATGTGCCTTT GGACTCTGGT 
GTCTCTGTGG TCCTTGTGGT AAATAACCTG GGTGGCCTGT CCTGTCTGGA GCTAGGTGTG 
GTGGCCGGCT CAGCTGTGCA CTGTCTGGAA AGCAGAGGTG TTCGCATTGC CCGAGCCCTG 
GTTGGCTCCT TCATGACTGC ACTGGAGATG GCTGGCGTCT CCCTCACCCT ACTGCTGGTG 
GATGACAAGC TCTTGAGACT GATTGATGCG GAGACCACTG CTGTAGCCTG GCCCAACCTA 
GCCAAAGCAT CTGTGACAGG TCGGAGCCGA GAGCTGGCAG CACCCAAGGG AGGATCTGAG 
TCAGAGGAGC CTGCCTCAGG CACCGCACCA TCCTGCCAGC ATACACACCT GGTCCTGGAG 
CGGGTGTGTG ACGTCCTTCT GGGCATGCAG GAGAAACTCA ATGAGCTGGA CCGCGCAGCA 
GGGGATGGGG ACTGCGGGAA CACACATGCC AGAGCTGCCA TAGCTATACA GGAATGGTTG 
AATGCAAGGC CTCCACCTGC CCAACCATCC CAGCTCCTCT CTGTCCTGGC CAGGCTGCTG 
CTGGAGAAGA TGGGGGGCTC CTCAGGGGTG CTGTATGGGC TGTTCCTTAC AGCAGCTGCA 
CGGCCACTGC AGAATTGCAG CAACCTTCCA GCCTGGGCTG ATGCCATGGA CACAGGCATT 
GAAGCCATGC AGCAGTATGG AGGAGCTGCG CCTGGGGACA GGACCATGCT GGATTCACTA 
TGTGCTGCTG CAGGGGAGCT GCATGCTTTG CGAGCCCCTG GTGCCGACCT GTTCCAGGTG 
CTGGCCAAGG CTGTCCAGAC ACGCTGCAGA GGAGGACCCT GA