HMBS cDNA ORF clone, Alligator mississippiensis(American alligator)

Gene
Clones
gRNAs

Summary

Gene Symbol	HMBS
Entrez Gene ID	102565829
Full Name	hydroxymethylbilane synthase
Gene Type	protein-coding
Organism	Alligator mississippiensis(American alligator)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006266834.3	XP_006266896.1	porphobilinogen deaminase isoform X1
XM_006266835.3	XP_006266897.1	porphobilinogen deaminase isoform X2

Pathways from BioSystems
Gene Ontology

The following HMBS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HMBS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAl52043	XM_006266834.3 Latest version!	Alligator mississippiensis hydroxymethylbilane synthase (HMBS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OAl52044	XM_006266835.3 Latest version!	Alligator mississippiensis hydroxymethylbilane synthase (HMBS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HMBS ( XM_006266834.3 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_006266896.1 Alligator mississippiensis hydroxymethylbilane synthase (HMBS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAl52043
Clone ID Related Accession (Same CDS sequence)	XM_006266834.3
Accession Version	XM_006266834.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	porphobilinogen deaminase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017708636.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 9, 2016 this sequence version replaced XM_006266834.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATT GTACATCAAG GAACAGCCAG GACGGAAATG GCCTGAAAGA CAGAATTATC 
CGAGTGGGCA CCAGGAAGAG CCAGCTGGCT CGGATTCAGA CTGACAGTGT GGTCGAGATG 
CTCTGCAAGC AATACCCTGG CCTTCACTTT GAGATAGTTG CCATGTCAAC AACTGGAGAT 
AAGATTCTGG ACACGGCTCT TTCCAAGATC GGGGAGAAGA GTCTTTTTAC CAAAGAGCTG 
GAAAATGCAC TAGAAAGAAA CGAAGTTGAC CTGGTGGTTC ACTCCTTGAA GGACCTGCCA 
ACATCGCTTC CCCCTGGCTT TACTATTGGT GCCATCTGCA AAAGGGAAAA CCCACATGAT 
GCTGTTGTGT TTCATCCTAA GAGCTGTGGG AAAACACTCA ATGCCCTCCC TGACAAGAGT 
GTCATTGGAA CCAGTTCACT TCGGCGTGCA GCACAGCTCA AAAGGAGCTT TCCTCAGCTG 
GAATTCAGGG ATATTAGAGG GAATTTAAAC ACCCGCTTAA AAAAGCTGGA TGAGAAGGAG 
GATTTCAGTG CTATAATCCT TGCTGCAGCT GGACTGAGGA GGATGGGCTG GGAGAATCGT 
ATTGGTCAGA TTCTAAACCC TGAGGATTGT CTGTATGCTG TTGGCCAGGG TGCCTTAGCA 
ATAGAAGTTC GAGCCAAAGA CCAAGAGATA CTGGATATGG TATCTGCCCT GCATGATGGG 
GAGACAGTGC TGTGCTGCAT TGCTGAGAGA GCCTTTATGA AACATCTGGA GGGGGGATGT 
AGCGTCCCTG TCGCAGTCAG CACCCTACTG AAAGACTCCC AGTTGTATTT GACAGGAGCT 
GTCTACAGCT TGGATGGTTC AGATAGCCTG AGAGAAACTA TGCAGACCAG TGTTAATTAC 
CCACAGCAGA ATGAAGATGG ACCCAATGAT GATGTGCAGC ACGTTGGTAT CACAGCCAAG 
AACATTCCTC ATCGTGCTCA AGAGGCTGCA GAGAAGCTAG GTACCGAACT AGCCAGCTTG 
CTGCTGAGCA AGGGAGCCAA GCACATCCTC AATGTGGCAA GGCAACTCAA TGATGCCCGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006266896.1
CDS	1183..2265
Translation	MENCTSRNSQDGNGLKDRIIRVGTRKSQLARIQTDSVVEMLCKQYPGLHFEIVAMSTTGDKILDTALSKIGEKSLFTKELENALERNEVDLVVHSLKDLPTSLPPGFTIGAICKRENPHDAVVFHPKSCGKTLNALPDKSVIGTSSLRRAAQLKRSFPQLEFRDIRGNLNTRLKKLDEKEDFSAIILAAAGLRRMGWENRIGQILNPEDCLYAVGQGALAIEVRAKDQEILDMVSALHDGETVLCCIAERAFMKHLEGGCSVPVAVSTLLKDSQLYLTGAVYSLDGSDSLRETMQTSVNYPQQNEDGPNDDVQHVGITAKNIPHRAQEAAEKLGTELASLLLSKGAKHILNVARQLNDAR

Target ORF information:

RefSeq Version	XM_006266834.3
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis hydroxymethylbilane synthase (HMBS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006266834.3

ORF Insert Sequence:

ATGGAAAATT GTACATCAAG GAACAGCCAG GACGGAAATG GCCTGAAAGA CAGAATTATC 
CGAGTGGGCA CCAGGAAGAG CCAGCTGGCT CGGATTCAGA CTGACAGTGT GGTCGAGATG 
CTCTGCAAGC AATACCCTGG CCTTCACTTT GAGATAGTTG CCATGTCAAC AACTGGAGAT 
AAGATTCTGG ACACGGCTCT TTCCAAGATC GGGGAGAAGA GTCTTTTTAC CAAAGAGCTG 
GAAAATGCAC TAGAAAGAAA CGAAGTTGAC CTGGTGGTTC ACTCCTTGAA GGACCTGCCA 
ACATCGCTTC CCCCTGGCTT TACTATTGGT GCCATCTGCA AAAGGGAAAA CCCACATGAT 
GCTGTTGTGT TTCATCCTAA GAGCTGTGGG AAAACACTCA ATGCCCTCCC TGACAAGAGT 
GTCATTGGAA CCAGTTCACT TCGGCGTGCA GCACAGCTCA AAAGGAGCTT TCCTCAGCTG 
GAATTCAGGG ATATTAGAGG GAATTTAAAC ACCCGCTTAA AAAAGCTGGA TGAGAAGGAG 
GATTTCAGTG CTATAATCCT TGCTGCAGCT GGACTGAGGA GGATGGGCTG GGAGAATCGT 
ATTGGTCAGA TTCTAAACCC TGAGGATTGT CTGTATGCTG TTGGCCAGGG TGCCTTAGCA 
ATAGAAGTTC GAGCCAAAGA CCAAGAGATA CTGGATATGG TATCTGCCCT GCATGATGGG 
GAGACAGTGC TGTGCTGCAT TGCTGAGAGA GCCTTTATGA AACATCTGGA GGGGGGATGT 
AGCGTCCCTG TCGCAGTCAG CACCCTACTG AAAGACTCCC AGTTGTATTT GACAGGAGCT 
GTCTACAGCT TGGATGGTTC AGATAGCCTG AGAGAAACTA TGCAGACCAG TGTTAATTAC 
CCACAGCAGA ATGAAGATGG ACCCAATGAT GATGTGCAGC ACGTTGGTAT CACAGCCAAG 
AACATTCCTC ATCGTGCTCA AGAGGCTGCA GAGAAGCTAG GTACCGAACT AGCCAGCTTG 
CTGCTGAGCA AGGGAGCCAA GCACATCCTC AATGTGGCAA GGCAACTCAA TGATGCCCGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMBS ( XM_006266835.3 ) cDNA ORF clone, Alligator mississippiensis(American alligator) -> XP_006266897.1 Alligator mississippiensis hydroxymethylbilane synthase (HMBS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAl52044
Clone ID Related Accession (Same CDS sequence)	XM_006266835.3
Accession Version	XM_006266835.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-08
Organism	Alligator mississippiensis(American alligator)
Product	porphobilinogen deaminase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017708636.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 9, 2016 this sequence version replaced XM_006266835.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator mississippiensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA CGGACGGAAA TGGCCTGAAA GACAGAATTA TCCGAGTGGG CACCAGGAAG 
AGCCAGCTGG CTCGGATTCA GACTGACAGT GTGGTCGAGA TGCTCTGCAA GCAATACCCT 
GGCCTTCACT TTGAGATAGT TGCCATGTCA ACAACTGGAG ATAAGATTCT GGACACGGCT 
CTTTCCAAGA TCGGGGAGAA GAGTCTTTTT ACCAAAGAGC TGGAAAATGC ACTAGAAAGA 
AACGAAGTTG ACCTGGTGGT TCACTCCTTG AAGGACCTGC CAACATCGCT TCCCCCTGGC 
TTTACTATTG GTGCCATCTG CAAAAGGGAA AACCCACATG ATGCTGTTGT GTTTCATCCT 
AAGAGCTGTG GGAAAACACT CAATGCCCTC CCTGACAAGA GTGTCATTGG AACCAGTTCA 
CTTCGGCGTG CAGCACAGCT CAAAAGGAGC TTTCCTCAGC TGGAATTCAG GGATATTAGA 
GGGAATTTAA ACACCCGCTT AAAAAAGCTG GATGAGAAGG AGGATTTCAG TGCTATAATC 
CTTGCTGCAG CTGGACTGAG GAGGATGGGC TGGGAGAATC GTATTGGTCA GATTCTAAAC 
CCTGAGGATT GTCTGTATGC TGTTGGCCAG GGTGCCTTAG CAATAGAAGT TCGAGCCAAA 
GACCAAGAGA TACTGGATAT GGTATCTGCC CTGCATGATG GGGAGACAGT GCTGTGCTGC 
ATTGCTGAGA GAGCCTTTAT GAAACATCTG GAGGGGGGAT GTAGCGTCCC TGTCGCAGTC 
AGCACCCTAC TGAAAGACTC CCAGTTGTAT TTGACAGGAG CTGTCTACAG CTTGGATGGT 
TCAGATAGCC TGAGAGAAAC TATGCAGACC AGTGTTAATT ACCCACAGCA GAATGAAGAT 
GGACCCAATG ATGATGTGCA GCACGTTGGT ATCACAGCCA AGAACATTCC TCATCGTGCT 
CAAGAGGCTG CAGAGAAGCT AGGTACCGAA CTAGCCAGCT TGCTGCTGAG CAAGGGAGCC 
AAGCACATCC TCAATGTGGC AAGGCAACTC AATGATGCCC GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006266897.1
CDS	95..1159
Translation	MSATDGNGLKDRIIRVGTRKSQLARIQTDSVVEMLCKQYPGLHFEIVAMSTTGDKILDTALSKIGEKSLFTKELENALERNEVDLVVHSLKDLPTSLPPGFTIGAICKRENPHDAVVFHPKSCGKTLNALPDKSVIGTSSLRRAAQLKRSFPQLEFRDIRGNLNTRLKKLDEKEDFSAIILAAAGLRRMGWENRIGQILNPEDCLYAVGQGALAIEVRAKDQEILDMVSALHDGETVLCCIAERAFMKHLEGGCSVPVAVSTLLKDSQLYLTGAVYSLDGSDSLRETMQTSVNYPQQNEDGPNDDVQHVGITAKNIPHRAQEAAEKLGTELASLLLSKGAKHILNVARQLNDAR

Target ORF information:

RefSeq Version	XM_006266835.3
Organism	Alligator mississippiensis(American alligator)
Definition	Alligator mississippiensis hydroxymethylbilane synthase (HMBS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006266835.3

ORF Insert Sequence:

ATGTCCGCCA CGGACGGAAA TGGCCTGAAA GACAGAATTA TCCGAGTGGG CACCAGGAAG 
AGCCAGCTGG CTCGGATTCA GACTGACAGT GTGGTCGAGA TGCTCTGCAA GCAATACCCT 
GGCCTTCACT TTGAGATAGT TGCCATGTCA ACAACTGGAG ATAAGATTCT GGACACGGCT 
CTTTCCAAGA TCGGGGAGAA GAGTCTTTTT ACCAAAGAGC TGGAAAATGC ACTAGAAAGA 
AACGAAGTTG ACCTGGTGGT TCACTCCTTG AAGGACCTGC CAACATCGCT TCCCCCTGGC 
TTTACTATTG GTGCCATCTG CAAAAGGGAA AACCCACATG ATGCTGTTGT GTTTCATCCT 
AAGAGCTGTG GGAAAACACT CAATGCCCTC CCTGACAAGA GTGTCATTGG AACCAGTTCA 
CTTCGGCGTG CAGCACAGCT CAAAAGGAGC TTTCCTCAGC TGGAATTCAG GGATATTAGA 
GGGAATTTAA ACACCCGCTT AAAAAAGCTG GATGAGAAGG AGGATTTCAG TGCTATAATC 
CTTGCTGCAG CTGGACTGAG GAGGATGGGC TGGGAGAATC GTATTGGTCA GATTCTAAAC 
CCTGAGGATT GTCTGTATGC TGTTGGCCAG GGTGCCTTAG CAATAGAAGT TCGAGCCAAA 
GACCAAGAGA TACTGGATAT GGTATCTGCC CTGCATGATG GGGAGACAGT GCTGTGCTGC 
ATTGCTGAGA GAGCCTTTAT GAAACATCTG GAGGGGGGAT GTAGCGTCCC TGTCGCAGTC 
AGCACCCTAC TGAAAGACTC CCAGTTGTAT TTGACAGGAG CTGTCTACAG CTTGGATGGT 
TCAGATAGCC TGAGAGAAAC TATGCAGACC AGTGTTAATT ACCCACAGCA GAATGAAGAT 
GGACCCAATG ATGATGTGCA GCACGTTGGT ATCACAGCCA AGAACATTCC TCATCGTGCT 
CAAGAGGCTG CAGAGAAGCT AGGTACCGAA CTAGCCAGCT TGCTGCTGAG CAAGGGAGCC 
AAGCACATCC TCAATGTGGC AAGGCAACTC AATGATGCCC GCTAA