ptpro cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis)

Gene
Clones
gRNAs

Summary

Gene Symbol	ptpro
Entrez Gene ID	110971392
Full Name	protein tyrosine phosphatase, receptor type O
Gene Type	protein-coding
Organism	Acanthochromis polyacanthus(spiny chromis)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022221734.1	XP_022077426.1	receptor-type tyrosine-protein phosphatase O isoform X2
XM_022221733.1	XP_022077425.1	receptor-type tyrosine-protein phosphatase O isoform X1

Pathways from BioSystems
Gene Ontology

The following ptpro gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ptpro cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc183026	XM_022221733.1 Latest version!	Acanthochromis polyacanthus protein tyrosine phosphatase, receptor type O (ptpro), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAc183027	XM_022221734.1 Latest version!	Acanthochromis polyacanthus protein tyrosine phosphatase, receptor type O (ptpro), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ptpro ( XM_022221733.1 ) cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis) -> XP_022077425.1 Acanthochromis polyacanthus protein tyrosine phosphatase, receptor type O (ptpro), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAc183026
Clone ID Related Accession (Same CDS sequence)	XM_022221733.1
Accession Version	XM_022221733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-06
Organism	Acanthochromis polyacanthus(spiny chromis)
Product	receptor-type tyrosine-protein phosphatase O isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019032312.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthochromis polyacanthus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACTC TGACGCTGCT CACAAAGCCA CTACCCATCC TCAGCGCTCA GATTTCTGAC 
TATAAAGAAG CTCCAGAAAC GGGAGTCGTG TTTGAAATCG ACCCTCCAGC TGGAAACGTC 
TTCAGCCGAG TGAACATCAG CTTCACCGAA GGACCAGAGA GACGGTCGAT GCTCTACAAA 
GATTTCTACA GAGGGAAGAC GGTGTTCAAA CATTGGTTAC CAGGGGTTTG CTACCGGAAC 
ATCACCTTCC AGCTCATCTC TGAAGCTACA GTTTACCAGA CGCCATTGGT CGCCCACAGT 
GACATCACAC ATACACCTCT GCACCACCGT ACAGTGCCCT TCCCTCCCCT CAACATCTCC 
CATAAGATTA TCCACCTGAG TCAGAGGGCA GCAGGTGAAG CCATCATAGC TCCGCTCCAC 
CAGACGGCTC GTAACGCCCG AGATGTTCCT CTGATGGAGG AGCGAGAGGA GGAGGAGGTT 
CAGCCGGAGG AGGAGAACAT CTCTGAGGAG GTGGCGACCT CCAGCCCCTT TAACTCCACC 
ATGGTGGTTA GCACCACCGC CACCGCTAAC AGCAGCCAGC CAGAGGAGGC GGAGCTTCAG 
AACTACTGGT TGGACCCGAC GGAGCCGTCG GCCGCCGACG ATGAGTTCGT GAACGCCGTG 
GTGTCGGAGT ACGAGGACTC CAACGAGCCG GGTTCAGCCA TGGGCCTCCA ACCAGAACCC 
TCCGTCCTGC CCACCAGGGT GCCCCCCATC CTGCTGGAGC TCCGCTGGCT GCCACCGAGG 
CCTCCGACCA GCCATGACGG CTTCAACGTC TACATCTACA GAGATGGAAA CTCCACAGAA 
ACAGCGACTG TAGACGAAAA CACTCACGAA TTCTTCACCG AGTTAACAGA ACCAGGAACG 
TACCGAGTCC AGGTCACCAC GCTGAGTTCA TCTGGAGACT GTGAGGCCCG AGAGAGCAGC 
GCTGACACCG GGTTCACCTT CTACCTCAGT CCTGGTGGTG AGCTGCTGGA GGAGCTTCGA 
GAACGTCCTC AGTCCGTTAG CGTCCGACTG TTGGACTCCA GCACCGCCGC CGTCTCCTGG 
GCGCCGTCCA ACCAGAACCA CAACGGCAGC CTGGTGTCGG TGGTTTCTAC CACCTGCCTG 
AAGCCGAGTC TGAGCCAGAG GATGGAGAAC ACCTACTGCA GCGAGGAGAA CTCAACCAGT 
GACATCATCA CCAACCTGAC GCCAGGTGCT CAGTACAGAG TCGTGGTTTT TCACACCAAC 
GGACCTCTGA TCAGCCCTCC GTCTGAGCCT GTCATCATCG ATATTGAGCC CACAGGTGTC 
CGTGAGCTGG CGGTCTATCC TCTGAGTCCG ACGGCCGTCA TCCTCAGCTG GCAGCGTCCG 
TACCACGTGG CCTTCAGGAA GTACGTCCTG CAGACCTTCT TCTTCAACCC TGTCACGCTG 
GCGTCGGAGT GGACCACCTA CTATGAGATC GCCGCCACCG CCTCAGTCAT CGCCTCCGTG 
AGGGTGACCG ACCTGCTGCC GGCGTGGTAT TACAACTTCC GTGTTTCCAT GGTGACGTGG 
GGCGACCCGC CGCTCAGCTG CTGCGACAGC TCAACCGTGA GCTTCGTTAC AGCTCCTGAA 
GCTCCTCACA TCTCCTCGGT GGATTATTCT CATGGAGTTT TATACGTCCG CTGGACTTAC 
GGAGAACTCT TCATCGACCT GTCGCACTCC AGGATGCTGC ACTGGCAGGT GGTGGCCGTC 
GGCAAGAAGG GACCCGAGAG GAGCTACTCA GTAGACGTGA CCCGTAACGT GATGCGAGCC 
CACCTGCCGC TGCCTCCAGG TGACATCTAC AACCTGACGG TGACGGCGTG CACAGAACGA 
AGCAGGAACA CGTCCACGCC CAACATCATC AAACTGGAAC CGGCTCCGCC CAGATCGCTG 
TTCGCCGTCA ACGCCACCCA CTCGTCCGTC ACTCTGCTGT GGACGGAGGA CGGCGTGGTC 
GACTACTACC AGGTGGTCTG CAGACCCAGC AGGAGCAGCA AGGAGCTCAA GACTCGAGAG 
CCTCAGACGT CGACGTCTCA CGTTGTGACG ATTTCCGGTT TGCTGCCGTC GTCAAGCTAC 
AACTGCACCG TGACCAGCTT CAGCTACAGC ACACCCAGCA AACCGGCCCA CATCAGCATC 
ACCACCATCG CTAAAGAGAT GAACCCCAGT GTAGCAGCCA TCTCGGCTCT AGCCGTCCTC 
AGCATACTGT TGATCAGCCT CCTGGTTCTG TTCCTGCTGG TTCTGAGGAA GAAACACCTG 
CAGATGACCA GAGAATGTGG CGCCGAGACC TTCGTCAACT TCGCGTCATT CGAGCGGGAC 
GGAAAACTTC CCTACAACTG GAGCAAGACC GCTTTAAAGA AACGGAAGCT AACAAGTCCT 
GTTCAGCTCG ACGACTTTGA CGCTTATTTT AAAGAAATGA GCAAAGATTC AGCCTATAAG 
TTCTCCCTGC AATTTGAGGA GCTGAAGAGT GTTGGTTTGG ATCTTTCCCA TGATGCAGCA 
GATCTTCCAG TCAACAGACC CAAGAACAGA TACACCAACA TCCTGCCCTA TGACTTCAGT 
CGTGTGAAGC TGATCTCGAT GCACAATGAC GAAGGTTCAG ATTACATCAA TGCCAACTAC 
ATTCCTGGCT ACAGACACTC CAGGGAGTAC ATCGCCACTC AGGGTCCACT GCCTGAAACC 
CGGAACGACT TCTGGAAAAT GGTTCTTCAG CAGAAATCTC CCATCATCGT GATGCTGACG 
CAGTGCAACG AACGGCGGAG GGTGAAGTGC GACCACTACT GGCCATTCAC CGATGAGCTG 
GTGATGTACG GTGAGATCAG CGTGGAGATG ATGTCAGAAA CCGAGTCTCC AGAGTGGACC 
ATCCGGAAGT TCAGGCTGGG ATACGCCGAT GAGACTCAGG ACGTCCTGCA CCTGAACTAC 
ACCTCGTGGC CAGACCACGG CGTCCCGACT GTCAACGCCA TCGAGTCCAT CCTGCAGTTC 
GTCCACATCG TCCGGCAGCA GGCCAACCGG ACCAAAGACC CCATCATCGT CCACTGCAGC 
GCCGGCGTCG GCAGAACCGG AACCTTCATG GCTCTGGACC GACTGATGCA GCAAATCAGG 
GAGCATGAGT TCATCGACAT CCTGGGGATG GTGTCAGAGA TGAGGTCACA CCGGCTGTCC 
ATGGTCCAGA CAGAGGAGCA GTACGTGTTC ATCCATCAGT GCGTCCTCCT CATGTGGCAG 
AAGAAGAAAC AGTCCAGCAT CACGTCAGAC GTCATCTATG AAAACGCCAG TAAAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022077425.1
CDS	104..3403
Translation	MKTLTLLTKPLPILSAQISDYKEAPETGVVFEIDPPAGNVFSRVNISFTEGPERRSMLYKDFYRGKTVFKHWLPGVCYRNITFQLISEATVYQTPLVAHSDITHTPLHHRTVPFPPLNISHKIIHLSQRAAGEAIIAPLHQTARNARDVPLMEEREEEEVQPEEENISEEVATSSPFNSTMVVSTTATANSSQPEEAELQNYWLDPTEPSAADDEFVNAVVSEYEDSNEPGSAMGLQPEPSVLPTRVPPILLELRWLPPRPPTSHDGFNVYIYRDGNSTETATVDENTHEFFTELTEPGTYRVQVTTLSSSGDCEARESSADTGFTFYLSPGGELLEELRERPQSVSVRLLDSSTAAVSWAPSNQNHNGSLVSVVSTTCLKPSLSQRMENTYCSEENSTSDIITNLTPGAQYRVVVFHTNGPLISPPSEPVIIDIEPTGVRELAVYPLSPTAVILSWQRPYHVAFRKYVLQTFFFNPVTLASEWTTYYEIAATASVIASVRVTDLLPAWYYNFRVSMVTWGDPPLSCCDSSTVSFVTAPEAPHISSVDYSHGVLYVRWTYGELFIDLSHSRMLHWQVVAVGKKGPERSYSVDVTRNVMRAHLPLPPGDIYNLTVTACTERSRNTSTPNIIKLEPAPPRSLFAVNATHSSVTLLWTEDGVVDYYQVVCRPSRSSKELKTREPQTSTSHVVTISGLLPSSSYNCTVTSFSYSTPSKPAHISITTIAKEMNPSVAAISALAVLSILLISLLVLFLLVLRKKHLQMTRECGAETFVNFASFERDGKLPYNWSKTALKKRKLTSPVQLDDFDAYFKEMSKDSAYKFSLQFEELKSVGLDLSHDAADLPVNRPKNRYTNILPYDFSRVKLISMHNDEGSDYINANYIPGYRHSREYIATQGPLPETRNDFWKMVLQQKSPIIVMLTQCNERRRVKCDHYWPFTDELVMYGEISVEMMSETESPEWTIRKFRLGYADETQDVLHLNYTSWPDHGVPTVNAIESILQFVHIVRQQANRTKDPIIVHCSAGVGRTGTFMALDRLMQQIREHEFIDILGMVSEMRSHRLSMVQTEEQYVFIHQCVLLMWQKKKQSSITSDVIYENASKT

Target ORF information:

RefSeq Version	XM_022221733.1
Organism	Acanthochromis polyacanthus(spiny chromis)
Definition	Acanthochromis polyacanthus protein tyrosine phosphatase, receptor type O (ptpro), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022221733.1

ORF Insert Sequence:

ATGAAGACTC TGACGCTGCT CACAAAGCCA CTACCCATCC TCAGCGCTCA GATTTCTGAC 
TATAAAGAAG CTCCAGAAAC GGGAGTCGTG TTTGAAATCG ACCCTCCAGC TGGAAACGTC 
TTCAGCCGAG TGAACATCAG CTTCACCGAA GGACCAGAGA GACGGTCGAT GCTCTACAAA 
GATTTCTACA GAGGGAAGAC GGTGTTCAAA CATTGGTTAC CAGGGGTTTG CTACCGGAAC 
ATCACCTTCC AGCTCATCTC TGAAGCTACA GTTTACCAGA CGCCATTGGT CGCCCACAGT 
GACATCACAC ATACACCTCT GCACCACCGT ACAGTGCCCT TCCCTCCCCT CAACATCTCC 
CATAAGATTA TCCACCTGAG TCAGAGGGCA GCAGGTGAAG CCATCATAGC TCCGCTCCAC 
CAGACGGCTC GTAACGCCCG AGATGTTCCT CTGATGGAGG AGCGAGAGGA GGAGGAGGTT 
CAGCCGGAGG AGGAGAACAT CTCTGAGGAG GTGGCGACCT CCAGCCCCTT TAACTCCACC 
ATGGTGGTTA GCACCACCGC CACCGCTAAC AGCAGCCAGC CAGAGGAGGC GGAGCTTCAG 
AACTACTGGT TGGACCCGAC GGAGCCGTCG GCCGCCGACG ATGAGTTCGT GAACGCCGTG 
GTGTCGGAGT ACGAGGACTC CAACGAGCCG GGTTCAGCCA TGGGCCTCCA ACCAGAACCC 
TCCGTCCTGC CCACCAGGGT GCCCCCCATC CTGCTGGAGC TCCGCTGGCT GCCACCGAGG 
CCTCCGACCA GCCATGACGG CTTCAACGTC TACATCTACA GAGATGGAAA CTCCACAGAA 
ACAGCGACTG TAGACGAAAA CACTCACGAA TTCTTCACCG AGTTAACAGA ACCAGGAACG 
TACCGAGTCC AGGTCACCAC GCTGAGTTCA TCTGGAGACT GTGAGGCCCG AGAGAGCAGC 
GCTGACACCG GGTTCACCTT CTACCTCAGT CCTGGTGGTG AGCTGCTGGA GGAGCTTCGA 
GAACGTCCTC AGTCCGTTAG CGTCCGACTG TTGGACTCCA GCACCGCCGC CGTCTCCTGG 
GCGCCGTCCA ACCAGAACCA CAACGGCAGC CTGGTGTCGG TGGTTTCTAC CACCTGCCTG 
AAGCCGAGTC TGAGCCAGAG GATGGAGAAC ACCTACTGCA GCGAGGAGAA CTCAACCAGT 
GACATCATCA CCAACCTGAC GCCAGGTGCT CAGTACAGAG TCGTGGTTTT TCACACCAAC 
GGACCTCTGA TCAGCCCTCC GTCTGAGCCT GTCATCATCG ATATTGAGCC CACAGGTGTC 
CGTGAGCTGG CGGTCTATCC TCTGAGTCCG ACGGCCGTCA TCCTCAGCTG GCAGCGTCCG 
TACCACGTGG CCTTCAGGAA GTACGTCCTG CAGACCTTCT TCTTCAACCC TGTCACGCTG 
GCGTCGGAGT GGACCACCTA CTATGAGATC GCCGCCACCG CCTCAGTCAT CGCCTCCGTG 
AGGGTGACCG ACCTGCTGCC GGCGTGGTAT TACAACTTCC GTGTTTCCAT GGTGACGTGG 
GGCGACCCGC CGCTCAGCTG CTGCGACAGC TCAACCGTGA GCTTCGTTAC AGCTCCTGAA 
GCTCCTCACA TCTCCTCGGT GGATTATTCT CATGGAGTTT TATACGTCCG CTGGACTTAC 
GGAGAACTCT TCATCGACCT GTCGCACTCC AGGATGCTGC ACTGGCAGGT GGTGGCCGTC 
GGCAAGAAGG GACCCGAGAG GAGCTACTCA GTAGACGTGA CCCGTAACGT GATGCGAGCC 
CACCTGCCGC TGCCTCCAGG TGACATCTAC AACCTGACGG TGACGGCGTG CACAGAACGA 
AGCAGGAACA CGTCCACGCC CAACATCATC AAACTGGAAC CGGCTCCGCC CAGATCGCTG 
TTCGCCGTCA ACGCCACCCA CTCGTCCGTC ACTCTGCTGT GGACGGAGGA CGGCGTGGTC 
GACTACTACC AGGTGGTCTG CAGACCCAGC AGGAGCAGCA AGGAGCTCAA GACTCGAGAG 
CCTCAGACGT CGACGTCTCA CGTTGTGACG ATTTCCGGTT TGCTGCCGTC GTCAAGCTAC 
AACTGCACCG TGACCAGCTT CAGCTACAGC ACACCCAGCA AACCGGCCCA CATCAGCATC 
ACCACCATCG CTAAAGAGAT GAACCCCAGT GTAGCAGCCA TCTCGGCTCT AGCCGTCCTC 
AGCATACTGT TGATCAGCCT CCTGGTTCTG TTCCTGCTGG TTCTGAGGAA GAAACACCTG 
CAGATGACCA GAGAATGTGG CGCCGAGACC TTCGTCAACT TCGCGTCATT CGAGCGGGAC 
GGAAAACTTC CCTACAACTG GAGCAAGACC GCTTTAAAGA AACGGAAGCT AACAAGTCCT 
GTTCAGCTCG ACGACTTTGA CGCTTATTTT AAAGAAATGA GCAAAGATTC AGCCTATAAG 
TTCTCCCTGC AATTTGAGGA GCTGAAGAGT GTTGGTTTGG ATCTTTCCCA TGATGCAGCA 
GATCTTCCAG TCAACAGACC CAAGAACAGA TACACCAACA TCCTGCCCTA TGACTTCAGT 
CGTGTGAAGC TGATCTCGAT GCACAATGAC GAAGGTTCAG ATTACATCAA TGCCAACTAC 
ATTCCTGGCT ACAGACACTC CAGGGAGTAC ATCGCCACTC AGGGTCCACT GCCTGAAACC 
CGGAACGACT TCTGGAAAAT GGTTCTTCAG CAGAAATCTC CCATCATCGT GATGCTGACG 
CAGTGCAACG AACGGCGGAG GGTGAAGTGC GACCACTACT GGCCATTCAC CGATGAGCTG 
GTGATGTACG GTGAGATCAG CGTGGAGATG ATGTCAGAAA CCGAGTCTCC AGAGTGGACC 
ATCCGGAAGT TCAGGCTGGG ATACGCCGAT GAGACTCAGG ACGTCCTGCA CCTGAACTAC 
ACCTCGTGGC CAGACCACGG CGTCCCGACT GTCAACGCCA TCGAGTCCAT CCTGCAGTTC 
GTCCACATCG TCCGGCAGCA GGCCAACCGG ACCAAAGACC CCATCATCGT CCACTGCAGC 
GCCGGCGTCG GCAGAACCGG AACCTTCATG GCTCTGGACC GACTGATGCA GCAAATCAGG 
GAGCATGAGT TCATCGACAT CCTGGGGATG GTGTCAGAGA TGAGGTCACA CCGGCTGTCC 
ATGGTCCAGA CAGAGGAGCA GTACGTGTTC ATCCATCAGT GCGTCCTCCT CATGTGGCAG 
AAGAAGAAAC AGTCCAGCAT CACGTCAGAC GTCATCTATG AAAACGCCAG TAAAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptpro ( XM_022221734.1 ) cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis) -> XP_022077426.1 Acanthochromis polyacanthus protein tyrosine phosphatase, receptor type O (ptpro), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAc183027
Clone ID Related Accession (Same CDS sequence)	XM_022221734.1
Accession Version	XM_022221734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-06
Organism	Acanthochromis polyacanthus(spiny chromis)
Product	receptor-type tyrosine-protein phosphatase O isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019032312.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthochromis polyacanthus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACTC TGACGCTGCT CACAAAGCCA CTACCCATCC TCAGCGCTCA GATTTCTGAC 
TATAAAGAAG CTCCAGAAAC GGGAGTCGTG TTTGAAATCG ACCCTCCAGC TGGAAACGTC 
TTCAGCCGAG TGAACATCAG CTTCACCGAA GGACCAGAGA GACGGTCGAT GCTCTACAAA 
GATTTCTACA GAGGGAAGAC GGTGTTCAAA CATTGGTTAC CAGGGGTTTG CTACCGGAAC 
ATCACCTTCC AGCTCATCTC TGAAGCTACA GTTTACCAGA CGCCATTGGT CGCCCACAGT 
GACATCACAC ATACACCTCT GCACCACCGT ACAGTGCCCT TCCCTCCCCT CAACATCTCC 
CATAAGATTA TCCACCTGAG TCAGAGGGCA GCAGGTGAAG CCATCATAGC TCCGCTCCAC 
CAGACGGCTC GTAACGCCCG AGATGTTCCT CTGATGGAGG AGCGAGAGGA GGAGGAGGTT 
CAGCCGGAGG AGGAGAACAT CTCTGAGGAG GTGGCGACCT CCAGCCCCTT TAACTCCACC 
ATGGTGGTTA GCACCACCGC CACCGCTAAC AGCAGCCAGC CAGAGGAGGC GGAGCTTCAG 
AACTACTGGT TGGACCCGAC GGAGCCGTCG GCCGCCGACG ATGAGTTCGT GAACGCCGTG 
GTGTCGGAGT ACGAGGACTC CAACGAGCCG GGTTCAGCCA TGGGCCTCCA ACCAGAACCC 
TCCGTCCTGC CCACCAGGGT GCCCCCCATC CTGCTGGAGC TCCGCTGGCT GCCACCGAGG 
CCTCCGACCA GCCATGACGG CTTCAACGTC TACATCTACA GAGATGGAAA CTCCACAGAA 
ACAGCGACTG TAGACGAAAA CACTCACGAA TTCTTCACCG AGTTAACAGA ACCAGGAACG 
TACCGAGTCC AGGTCACCAC GCTGAGTTCA TCTGGAGACT GTGAGGCCCG AGAGAGCAGC 
GCTGACACCG GGTTCACCTT CTACCTCAGT CCTGGTGGTG AGCTGCTGGA GGAGCTTCGA 
GAACGTCCTC AGTCCGTTAG CGTCCGACTG TTGGACTCCA GCACCGCCGC CGTCTCCTGG 
GCGCCGTCCA ACCAGAACCA CAACGGCAGC CTGGTGTCGG TGGTTTCTAC CACCTGCCTG 
AAGCCGAGTC TGAGCCAGAG GATGGAGAAC ACCTACTGCA GCGAGGAGAA CTCAACCAGT 
GACATCATCA CCAACCTGAC GCCAGGTGCT CAGTACAGAG TCGTGGTTTT TCACACCAAC 
GGACCTCTGA TCAGCCCTCC GTCTGAGCCT GTCATCATCG ATATTGAGCC CACAGGTGTC 
CGTGAGCTGG CGGTCTATCC TCTGAGTCCG ACGGCCGTCA TCCTCAGCTG GCAGCGTCCG 
TACCACGTGG CCTTCAGGAA GTACGTCCTG CAGACCTTCT TCTTCAACCC TGTCACGCTG 
GCGTCGGAGT GGACCACCTA CTATGAGATC GCCGCCACCG CCTCAGTCAT CGCCTCCGTG 
AGGGTGACCG ACCTGCTGCC GGCGTGGTAT TACAACTTCC GTGTTTCCAT GGTGACGTGG 
GGCGACCCGC CGCTCAGCTG CTGCGACAGC TCAACCGTGA GCTTCGTTAC AGCTCCTGAA 
GCTCCTCACA TCTCCTCGGT GGATTATTCT CATGGAGTTT TATACGTCCG CTGGACTTAC 
GGAGAACTCT TCATCGACCT GTCGCACTCC AGGATGCTGC ACTGGCAGGT GGTGGCCGTC 
GGCAAGAAGG GACCCGAGAG GAGCTACTCA GTAGACGTGA CCCGTAACGT GATGCGAGCC 
CACCTGCCGC TGCCTCCAGG TGACATCTAC AACCTGACGG TGACGGCGTG CACAGAACGA 
AGCAGGAACA CGTCCACGCC CAACATCATC AAACTGGAAC CGGCTCCGCC CAGATCGCTG 
TTCGCCGTCA ACGCCACCCA CTCGTCCGTC ACTCTGCTGT GGACGGAGGA CGGCGTGGTC 
GACTACTACC AGGTGGTCTG CAGACCCAGC AGGAGCAGCA AGGAGCTCAA GACTCGAGAG 
CCTCAGACGT CGACGTCTCA CGTTGTGACG ATTTCCGGTT TGCTGCCGTC GTCAAGCTAC 
AACTGCACCG TGACCAGCTT CAGCTACAGC ACACCCAGCA AACCGGCCCA CATCAGCATC 
ACCACCATCG CTAAAGAGAT GAACCCCAGT GTAGCAGCCA TCTCGGCTCT AGCCGTCCTC 
AGCATACTGT TGATCAGCCT CCTGGTTCTG TTCCTGCTGG TTCTGAGGAA GAAACACCTG 
CAGATGACCA GAGAATGTGG CGCCGAGACC TTCGTCAACT TCGCGTCATT CGAGCGGGAC 
GGAAAACTTC CCTACAACTG TCCTGTTCAG CTCGACGACT TTGACGCTTA TTTTAAAGAA 
ATGAGCAAAG ATTCAGCCTA TAAGTTCTCC CTGCAATTTG AGGAGCTGAA GAGTGTTGGT 
TTGGATCTTT CCCATGATGC AGCAGATCTT CCAGTCAACA GACCCAAGAA CAGATACACC 
AACATCCTGC CCTATGACTT CAGTCGTGTG AAGCTGATCT CGATGCACAA TGACGAAGGT 
TCAGATTACA TCAATGCCAA CTACATTCCT GGCTACAGAC ACTCCAGGGA GTACATCGCC 
ACTCAGGGTC CACTGCCTGA AACCCGGAAC GACTTCTGGA AAATGGTTCT TCAGCAGAAA 
TCTCCCATCA TCGTGATGCT GACGCAGTGC AACGAACGGC GGAGGGTGAA GTGCGACCAC 
TACTGGCCAT TCACCGATGA GCTGGTGATG TACGGTGAGA TCAGCGTGGA GATGATGTCA 
GAAACCGAGT CTCCAGAGTG GACCATCCGG AAGTTCAGGC TGGGATACGC CGATGAGACT 
CAGGACGTCC TGCACCTGAA CTACACCTCG TGGCCAGACC ACGGCGTCCC GACTGTCAAC 
GCCATCGAGT CCATCCTGCA GTTCGTCCAC ATCGTCCGGC AGCAGGCCAA CCGGACCAAA 
GACCCCATCA TCGTCCACTG CAGCGCCGGC GTCGGCAGAA CCGGAACCTT CATGGCTCTG 
GACCGACTGA TGCAGCAAAT CAGGGAGCAT GAGTTCATCG ACATCCTGGG GATGGTGTCA 
GAGATGAGGT CACACCGGCT GTCCATGGTC CAGACAGAGG AGCAGTACGT GTTCATCCAT 
CAGTGCGTCC TCCTCATGTG GCAGAAGAAG AAACAGTCCA GCATCACGTC AGACGTCATC 
TATGAAAACG CCAGTAAAAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022077426.1
CDS	104..3367
Translation	MKTLTLLTKPLPILSAQISDYKEAPETGVVFEIDPPAGNVFSRVNISFTEGPERRSMLYKDFYRGKTVFKHWLPGVCYRNITFQLISEATVYQTPLVAHSDITHTPLHHRTVPFPPLNISHKIIHLSQRAAGEAIIAPLHQTARNARDVPLMEEREEEEVQPEEENISEEVATSSPFNSTMVVSTTATANSSQPEEAELQNYWLDPTEPSAADDEFVNAVVSEYEDSNEPGSAMGLQPEPSVLPTRVPPILLELRWLPPRPPTSHDGFNVYIYRDGNSTETATVDENTHEFFTELTEPGTYRVQVTTLSSSGDCEARESSADTGFTFYLSPGGELLEELRERPQSVSVRLLDSSTAAVSWAPSNQNHNGSLVSVVSTTCLKPSLSQRMENTYCSEENSTSDIITNLTPGAQYRVVVFHTNGPLISPPSEPVIIDIEPTGVRELAVYPLSPTAVILSWQRPYHVAFRKYVLQTFFFNPVTLASEWTTYYEIAATASVIASVRVTDLLPAWYYNFRVSMVTWGDPPLSCCDSSTVSFVTAPEAPHISSVDYSHGVLYVRWTYGELFIDLSHSRMLHWQVVAVGKKGPERSYSVDVTRNVMRAHLPLPPGDIYNLTVTACTERSRNTSTPNIIKLEPAPPRSLFAVNATHSSVTLLWTEDGVVDYYQVVCRPSRSSKELKTREPQTSTSHVVTISGLLPSSSYNCTVTSFSYSTPSKPAHISITTIAKEMNPSVAAISALAVLSILLISLLVLFLLVLRKKHLQMTRECGAETFVNFASFERDGKLPYNCPVQLDDFDAYFKEMSKDSAYKFSLQFEELKSVGLDLSHDAADLPVNRPKNRYTNILPYDFSRVKLISMHNDEGSDYINANYIPGYRHSREYIATQGPLPETRNDFWKMVLQQKSPIIVMLTQCNERRRVKCDHYWPFTDELVMYGEISVEMMSETESPEWTIRKFRLGYADETQDVLHLNYTSWPDHGVPTVNAIESILQFVHIVRQQANRTKDPIIVHCSAGVGRTGTFMALDRLMQQIREHEFIDILGMVSEMRSHRLSMVQTEEQYVFIHQCVLLMWQKKKQSSITSDVIYENASKT

Target ORF information:

RefSeq Version	XM_022221734.1
Organism	Acanthochromis polyacanthus(spiny chromis)
Definition	Acanthochromis polyacanthus protein tyrosine phosphatase, receptor type O (ptpro), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022221734.1

ORF Insert Sequence:

ATGAAGACTC TGACGCTGCT CACAAAGCCA CTACCCATCC TCAGCGCTCA GATTTCTGAC 
TATAAAGAAG CTCCAGAAAC GGGAGTCGTG TTTGAAATCG ACCCTCCAGC TGGAAACGTC 
TTCAGCCGAG TGAACATCAG CTTCACCGAA GGACCAGAGA GACGGTCGAT GCTCTACAAA 
GATTTCTACA GAGGGAAGAC GGTGTTCAAA CATTGGTTAC CAGGGGTTTG CTACCGGAAC 
ATCACCTTCC AGCTCATCTC TGAAGCTACA GTTTACCAGA CGCCATTGGT CGCCCACAGT 
GACATCACAC ATACACCTCT GCACCACCGT ACAGTGCCCT TCCCTCCCCT CAACATCTCC 
CATAAGATTA TCCACCTGAG TCAGAGGGCA GCAGGTGAAG CCATCATAGC TCCGCTCCAC 
CAGACGGCTC GTAACGCCCG AGATGTTCCT CTGATGGAGG AGCGAGAGGA GGAGGAGGTT 
CAGCCGGAGG AGGAGAACAT CTCTGAGGAG GTGGCGACCT CCAGCCCCTT TAACTCCACC 
ATGGTGGTTA GCACCACCGC CACCGCTAAC AGCAGCCAGC CAGAGGAGGC GGAGCTTCAG 
AACTACTGGT TGGACCCGAC GGAGCCGTCG GCCGCCGACG ATGAGTTCGT GAACGCCGTG 
GTGTCGGAGT ACGAGGACTC CAACGAGCCG GGTTCAGCCA TGGGCCTCCA ACCAGAACCC 
TCCGTCCTGC CCACCAGGGT GCCCCCCATC CTGCTGGAGC TCCGCTGGCT GCCACCGAGG 
CCTCCGACCA GCCATGACGG CTTCAACGTC TACATCTACA GAGATGGAAA CTCCACAGAA 
ACAGCGACTG TAGACGAAAA CACTCACGAA TTCTTCACCG AGTTAACAGA ACCAGGAACG 
TACCGAGTCC AGGTCACCAC GCTGAGTTCA TCTGGAGACT GTGAGGCCCG AGAGAGCAGC 
GCTGACACCG GGTTCACCTT CTACCTCAGT CCTGGTGGTG AGCTGCTGGA GGAGCTTCGA 
GAACGTCCTC AGTCCGTTAG CGTCCGACTG TTGGACTCCA GCACCGCCGC CGTCTCCTGG 
GCGCCGTCCA ACCAGAACCA CAACGGCAGC CTGGTGTCGG TGGTTTCTAC CACCTGCCTG 
AAGCCGAGTC TGAGCCAGAG GATGGAGAAC ACCTACTGCA GCGAGGAGAA CTCAACCAGT 
GACATCATCA CCAACCTGAC GCCAGGTGCT CAGTACAGAG TCGTGGTTTT TCACACCAAC 
GGACCTCTGA TCAGCCCTCC GTCTGAGCCT GTCATCATCG ATATTGAGCC CACAGGTGTC 
CGTGAGCTGG CGGTCTATCC TCTGAGTCCG ACGGCCGTCA TCCTCAGCTG GCAGCGTCCG 
TACCACGTGG CCTTCAGGAA GTACGTCCTG CAGACCTTCT TCTTCAACCC TGTCACGCTG 
GCGTCGGAGT GGACCACCTA CTATGAGATC GCCGCCACCG CCTCAGTCAT CGCCTCCGTG 
AGGGTGACCG ACCTGCTGCC GGCGTGGTAT TACAACTTCC GTGTTTCCAT GGTGACGTGG 
GGCGACCCGC CGCTCAGCTG CTGCGACAGC TCAACCGTGA GCTTCGTTAC AGCTCCTGAA 
GCTCCTCACA TCTCCTCGGT GGATTATTCT CATGGAGTTT TATACGTCCG CTGGACTTAC 
GGAGAACTCT TCATCGACCT GTCGCACTCC AGGATGCTGC ACTGGCAGGT GGTGGCCGTC 
GGCAAGAAGG GACCCGAGAG GAGCTACTCA GTAGACGTGA CCCGTAACGT GATGCGAGCC 
CACCTGCCGC TGCCTCCAGG TGACATCTAC AACCTGACGG TGACGGCGTG CACAGAACGA 
AGCAGGAACA CGTCCACGCC CAACATCATC AAACTGGAAC CGGCTCCGCC CAGATCGCTG 
TTCGCCGTCA ACGCCACCCA CTCGTCCGTC ACTCTGCTGT GGACGGAGGA CGGCGTGGTC 
GACTACTACC AGGTGGTCTG CAGACCCAGC AGGAGCAGCA AGGAGCTCAA GACTCGAGAG 
CCTCAGACGT CGACGTCTCA CGTTGTGACG ATTTCCGGTT TGCTGCCGTC GTCAAGCTAC 
AACTGCACCG TGACCAGCTT CAGCTACAGC ACACCCAGCA AACCGGCCCA CATCAGCATC 
ACCACCATCG CTAAAGAGAT GAACCCCAGT GTAGCAGCCA TCTCGGCTCT AGCCGTCCTC 
AGCATACTGT TGATCAGCCT CCTGGTTCTG TTCCTGCTGG TTCTGAGGAA GAAACACCTG 
CAGATGACCA GAGAATGTGG CGCCGAGACC TTCGTCAACT TCGCGTCATT CGAGCGGGAC 
GGAAAACTTC CCTACAACTG TCCTGTTCAG CTCGACGACT TTGACGCTTA TTTTAAAGAA 
ATGAGCAAAG ATTCAGCCTA TAAGTTCTCC CTGCAATTTG AGGAGCTGAA GAGTGTTGGT 
TTGGATCTTT CCCATGATGC AGCAGATCTT CCAGTCAACA GACCCAAGAA CAGATACACC 
AACATCCTGC CCTATGACTT CAGTCGTGTG AAGCTGATCT CGATGCACAA TGACGAAGGT 
TCAGATTACA TCAATGCCAA CTACATTCCT GGCTACAGAC ACTCCAGGGA GTACATCGCC 
ACTCAGGGTC CACTGCCTGA AACCCGGAAC GACTTCTGGA AAATGGTTCT TCAGCAGAAA 
TCTCCCATCA TCGTGATGCT GACGCAGTGC AACGAACGGC GGAGGGTGAA GTGCGACCAC 
TACTGGCCAT TCACCGATGA GCTGGTGATG TACGGTGAGA TCAGCGTGGA GATGATGTCA 
GAAACCGAGT CTCCAGAGTG GACCATCCGG AAGTTCAGGC TGGGATACGC CGATGAGACT 
CAGGACGTCC TGCACCTGAA CTACACCTCG TGGCCAGACC ACGGCGTCCC GACTGTCAAC 
GCCATCGAGT CCATCCTGCA GTTCGTCCAC ATCGTCCGGC AGCAGGCCAA CCGGACCAAA 
GACCCCATCA TCGTCCACTG CAGCGCCGGC GTCGGCAGAA CCGGAACCTT CATGGCTCTG 
GACCGACTGA TGCAGCAAAT CAGGGAGCAT GAGTTCATCG ACATCCTGGG GATGGTGTCA 
GAGATGAGGT CACACCGGCT GTCCATGGTC CAGACAGAGG AGCAGTACGT GTTCATCCAT 
CAGTGCGTCC TCCTCATGTG GCAGAAGAAG AAACAGTCCA GCATCACGTC AGACGTCATC 
TATGAAAACG CCAGTAAAAC ATAA