LOC110957548 cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC110957548
Entrez Gene ID	110957548
Full Name	attractin-like protein 1
Gene Type	protein-coding
Organism	Acanthochromis polyacanthus(spiny chromis)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022203606.1	XP_022059298.1	attractin-like protein 1

Pathways from BioSystems
Gene Ontology

The following LOC110957548 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC110957548 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc199677	XM_022203606.1 Latest version!	Acanthochromis polyacanthus attractin-like protein 1 (LOC110957548), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC110957548 ( XM_022203606.1 ) cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis) -> XP_022059298.1 Acanthochromis polyacanthus attractin-like protein 1 (LOC110957548), mRNA.

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CloneID	OAc199677
Clone ID Related Accession (Same CDS sequence)	XM_022203606.1
Accession Version	XM_022203606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-06
Organism	Acanthochromis polyacanthus(spiny chromis)
Product	attractin-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019029658.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthochromis polyacanthus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGATGACA CCGGGGCGGA ACATCTCTGC GCCTGGACCG GGGCGTCCAG ACGGAGCGTC 
CGGGACGTTC TGAGGACCTA CCTGGGGATA CTGATGGTGC TTCACCTCGT GCTGCACACG 
GAAGCCTCAT CTGTGTCCAA GTCGTGTGAT AAAAACTGCT TTTCAGGGAA GTGCGTGAAC 
GGATCCTGCG TGTGTGACCA CGGCTGGGTG GGAGATCAGT GCCAACACTG TCAGGGGAGA 
TTCAAGTTAA CAGACCTGTC AGGTTCTCTC ACAGATGGTC CAGTAAACTA CAAATACAAG 
ACCAAATGCA CTTGGTTAAT AGAAGGATTC CCTAATGCAG TACTCAGGCT GCGCTTCAAT 
CACTTTGCCA CTGAGTGCAG CTGGGACCAC ATGTACGTCT ATGATGGAGA CTCCATTTAC 
GCCCCTTTGA TTGCCGTCTT CAGTGGTCTG GTGGTACCGG AGTCCAGCGG GAATGAAACG 
GTACCGGAGG TGGTGACGAC GTCCGGCTAC GCTCTGCTTC ACTTCTTCAG CGATGCTGCC 
TACAACCTGA CTGGTTTCAG CATCTCCTAC TCGATAAACT CTTGTCCCAA CAACTGCTCG 
GGCCACGGTC GATGCAGCAC GGCCAACTCG GTGGCGGGTC GGGTGTACTG CGAGTGCGAC 
GAGTACTGGA AAGGAGAGGC GTGTGACATC CCATACTGCA GAGACAACTG TGGCAGCCCC 
GACCACGGCT ACTGCGACCT GACTGGGGAG AAGCTGTGCG TCTGCAATGA CAGCTGGCAA 
GGTCCAGACT GCTCTCTCTC CGTCCCCTCC ACTGAGGCCT TCTGGGTTCT ACCCAGCATC 
AAACCATCGG CTCAGTCTCT GGGTCGGGCG TCCCACCGGG CCCTGGTGCA TTCTGGGTTG 
ATGTGGGTGG TGGGCGGCTA CTCCTTCAAC TACTCCAACT ACCACATGGT GCTCAACTAC 
AACCTGGAGA CGAGTACGTG GGATGTTGTT CCTGTCAACA ACGGTCCTCA CTACAGATAT 
GGACACTCAC TGGCTCTGTA CCAGGACGAC ATCTACATGT TTGGTGGGAA GCTGGAATCG 
GGATCCGCCA ACGTGACGGA TGAGTTGTGG GTGTTCAACA TCCCCAGACG CACCTGGTCG 
CCACGGAAAC CAGCCCCGCC TCCCCCGTAC GGCCTGGAGG GCCACACGGC CCACGTTGTG 
GAGCTGGCTG ACGGCGAGCC GGTGATGCTC GTCTTTTTTG GATACTCACC GATCTACAGC 
TACATCAACA AGGTCCAGGA GTACAACATC AGGTCCAACT CGTGGCAGGT GGTTTCGGCC 
GGAGGCGCCG TGGTCCAGGG AGGTTACGGC CACAGCAGCG TGTTGGACGA GGCCAGCGGC 
TGCGTGTTCG TCCACGGAGG CTACAAGGCT CTGAGCAACA ACAAATACGG CCTGGTGGAC 
CACATGTACC GATACCACGT CCACACCAAG ACATGGTTGA TCCTCAGGGA GAGCGGCTTG 
GCTCGGTATC TCCACTCGGT GGTGCTGTTG AGCGGCACAC TGCTGGTCTT TGGAGGAAAC 
ACCCACAATG ATACGTCTCT GAGCAACGGA GCCAAGTGTT TTTCAGCCGA CTTTCTGGCG 
TACGATATTG CCTGCGATGA GTGGAGCGTC CTCCCCAGGC CTGGACTGCA CCGAGACATC 
AACCGCTTCG GACACTCTGC TGTGGTCAGC AACGGCTCCA TGTACATCTT TGGAGGTTTT 
TCGGGTCTCC TTCTGAACGA CGTCCTGGCC TACACCCCTC CGTCCTGCCT GGCCTTCTCC 
AGCCAAGCTT CGTGTGCCGC CGCTGGGCCT GGACTTCGCT GCCACTGGGT CAAAAGTAGC 
TGCGTCCCCT GGGAGCCCAA ACCACCGGAC CACGCTTTCC CTGCTCCTTT CTGCCCTGCA 
CAACCCGACA CTACAGACGA ACAGTGTTTC CGGTTCTCAG ACTGTGCGAG CTGCACGGCC 
AACACCAGAG GATGCCAGTG GTGTGAAGAC AGGAAGTGTA TCTCCGCCTC CAGCAACTGC 
ACTGTGCTGA CTCTGGAACT GGCCCAGCAG CATGGCGGCG CTCTGTCTCT GTCTCCACCT 
CCATCCATGT TGTCTGAGCA TCAGCTGAGC TTGTGGAAGC ACGGAGCCAG AAGGAGAGGA 
CATTCGGTGA CAGACTCGTC CAGGTGTAAA CGCAGGGAGG AGCAGGAGTG TTTCCGACTG 
GCCAACTGCA GGAGCTGCTC CCTGAACGCC AACTGTCAGT GGGAGGCGCA GCAGCAGGAG 
TGCCAGGCGC TGCCAGGTAA TCTGTGTGGG GAGGGTTGGC AGCACGTCGG AGAGGCCTGC 
CTGAGGATCA ACTCCAGCAG GGAGAGCTAC GATAACGCCC AGCACTACTG CAAGAACCTG 
GGAGGGAACA TCGCCTCTCT GCTCACTGAC AAACAGGTCA ATTTTGTCCT GGAGGAGCTG 
CAGAAGTATC AGCTGCAGGA CAAGAGGTTA TCTCCATGGG TCGGCCTGAG GAAGATCAAC 
GTGTCGTACT GGGGCTGGGA GGACGCCTCA CCGTTCACCA ACACCAGCCT GAGGTGGTCA 
CCTGGAGAGC CCAGCGACTC TGGATTTTGT GCTTACCTGG AGCGAGCGCA GGTGGCCGGT 
CTGAAGGCAA ACCCCTGCAC GGCCACTACC GACGGCCTGA TCTGTGAAAA GCCTGCCGGG 
AGTCCACAGA GCCAGAGCGC TCGTCCCTGT AAGACGCCGT GCGCGCTACG TAGCAGCTGT 
GCTAACTGCA CCAGCCAGGC CATGGAGTGT ATGTGGTGTG GAAGCACTCA GCGATGCGTC 
GACTCCTCGG CCTACGTCAT CTCCTTTCCC TACGGACAGT GTCTGGAGTG GCAGACCCAG 
GACTGCTCAG CCCAGAACTG TTCCGGTCTA AGGACTTGTT CCGACTGTCT GGAGAGGGTT 
GAGTGCGGCT GGTGTGGAGA TCCCAGTAAC ACCGGCAGAG GTGTCTGCAT GGAGGGCTCC 
TACCGAGGCC CCCTGAAGCC CCTGAACCCG AGGGCCGGGC CCAGAGACAG GGAGAGGCTA 
CGAGACCGGG ACATGGTGGT GGATCAGAAC CTGTGTTCAT CCGATCGAGG CTACAACTGG 
GCATTTATCC AGTGTCCCGC GTGTCAGTGT AACGGCCACA GCAGGTGTGT GAACGGCAGC 
TTGTGTGAAC ACTGCGGGAA CCTGACGGCG GGAACGCACT GCCAGAGCTG CATGCCCGGT 
TACTATGGCG ACCCCACCAA TGGCGGGAAG TGCAACGCGT GCAAGTGCAA CAGTCACGCC 
AACGTCTGCC ACGTCAACAC GGGGAAGTGC TTCTGTACCA CCAAGGGCGT GAAGGGAGAC 
CAGTGTCAGC TATGTGACTC AGAAAACCGT TACCTTGGCA ACCCTCTCCG AGGAACATGT 
TATTACAACC TGTTGATTGA TTACCAGTTT ACCTTCAGTC TGCTGCAGGA AGACGACCGT 
TACTACACCG CCATCAACTT CATGGCCACA CCTGAACAGG CCAACAAAAA CCTGGACATG 
TCCATCAACG CCTCCAACAA CTTCAACCTC AACATCACTT GGTCCTTAAG CTCCACAGCT 
GGAACCATCT CCGGTGAAGA GATGCCCATC ATAGCGAGGA CCAACATCAA GGAGCACAGA 
GACAGTTTCT CCTGTGAGAA GTTCAACTTC AGACTCCATC ACAACATCAC CTTCTACGTC 
TACGTGTCCA ACTTCAGCTG GCCCATCAAA ATCCAGATTG CGTTCTCGCA GCACAACAGC 
ATCATGGACC TGGTGCAGTT CTTCGTTACC TTCTTTAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022059298.1
CDS	572..4393
Translation	MDDTGAEHLCAWTGASRRSVRDVLRTYLGILMVLHLVLHTEASSVSKSCDKNCFSGKCVNGSCVCDHGWVGDQCQHCQGRFKLTDLSGSLTDGPVNYKYKTKCTWLIEGFPNAVLRLRFNHFATECSWDHMYVYDGDSIYAPLIAVFSGLVVPESSGNETVPEVVTTSGYALLHFFSDAAYNLTGFSISYSINSCPNNCSGHGRCSTANSVAGRVYCECDEYWKGEACDIPYCRDNCGSPDHGYCDLTGEKLCVCNDSWQGPDCSLSVPSTEAFWVLPSIKPSAQSLGRASHRALVHSGLMWVVGGYSFNYSNYHMVLNYNLETSTWDVVPVNNGPHYRYGHSLALYQDDIYMFGGKLESGSANVTDELWVFNIPRRTWSPRKPAPPPPYGLEGHTAHVVELADGEPVMLVFFGYSPIYSYINKVQEYNIRSNSWQVVSAGGAVVQGGYGHSSVLDEASGCVFVHGGYKALSNNKYGLVDHMYRYHVHTKTWLILRESGLARYLHSVVLLSGTLLVFGGNTHNDTSLSNGAKCFSADFLAYDIACDEWSVLPRPGLHRDINRFGHSAVVSNGSMYIFGGFSGLLLNDVLAYTPPSCLAFSSQASCAAAGPGLRCHWVKSSCVPWEPKPPDHAFPAPFCPAQPDTTDEQCFRFSDCASCTANTRGCQWCEDRKCISASSNCTVLTLELAQQHGGALSLSPPPSMLSEHQLSLWKHGARRRGHSVTDSSRCKRREEQECFRLANCRSCSLNANCQWEAQQQECQALPGNLCGEGWQHVGEACLRINSSRESYDNAQHYCKNLGGNIASLLTDKQVNFVLEELQKYQLQDKRLSPWVGLRKINVSYWGWEDASPFTNTSLRWSPGEPSDSGFCAYLERAQVAGLKANPCTATTDGLICEKPAGSPQSQSARPCKTPCALRSSCANCTSQAMECMWCGSTQRCVDSSAYVISFPYGQCLEWQTQDCSAQNCSGLRTCSDCLERVECGWCGDPSNTGRGVCMEGSYRGPLKPLNPRAGPRDRERLRDRDMVVDQNLCSSDRGYNWAFIQCPACQCNGHSRCVNGSLCEHCGNLTAGTHCQSCMPGYYGDPTNGGKCNACKCNSHANVCHVNTGKCFCTTKGVKGDQCQLCDSENRYLGNPLRGTCYYNLLIDYQFTFSLLQEDDRYYTAINFMATPEQANKNLDMSINASNNFNLNITWSLSSTAGTISGEEMPIIARTNIKEHRDSFSCEKFNFRLHHNITFYVYVSNFSWPIKIQIAFSQHNSIMDLVQFFVTFFR

Target ORF information:

RefSeq Version	XM_022203606.1
Organism	Acanthochromis polyacanthus(spiny chromis)
Definition	Acanthochromis polyacanthus attractin-like protein 1 (LOC110957548), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022203606.1

ORF Insert Sequence:

ATGGATGACA CCGGGGCGGA ACATCTCTGC GCCTGGACCG GGGCGTCCAG ACGGAGCGTC 
CGGGACGTTC TGAGGACCTA CCTGGGGATA CTGATGGTGC TTCACCTCGT GCTGCACACG 
GAAGCCTCAT CTGTGTCCAA GTCGTGTGAT AAAAACTGCT TTTCAGGGAA GTGCGTGAAC 
GGATCCTGCG TGTGTGACCA CGGCTGGGTG GGAGATCAGT GCCAACACTG TCAGGGGAGA 
TTCAAGTTAA CAGACCTGTC AGGTTCTCTC ACAGATGGTC CAGTAAACTA CAAATACAAG 
ACCAAATGCA CTTGGTTAAT AGAAGGATTC CCTAATGCAG TACTCAGGCT GCGCTTCAAT 
CACTTTGCCA CTGAGTGCAG CTGGGACCAC ATGTACGTCT ATGATGGAGA CTCCATTTAC 
GCCCCTTTGA TTGCCGTCTT CAGTGGTCTG GTGGTACCGG AGTCCAGCGG GAATGAAACG 
GTACCGGAGG TGGTGACGAC GTCCGGCTAC GCTCTGCTTC ACTTCTTCAG CGATGCTGCC 
TACAACCTGA CTGGTTTCAG CATCTCCTAC TCGATAAACT CTTGTCCCAA CAACTGCTCG 
GGCCACGGTC GATGCAGCAC GGCCAACTCG GTGGCGGGTC GGGTGTACTG CGAGTGCGAC 
GAGTACTGGA AAGGAGAGGC GTGTGACATC CCATACTGCA GAGACAACTG TGGCAGCCCC 
GACCACGGCT ACTGCGACCT GACTGGGGAG AAGCTGTGCG TCTGCAATGA CAGCTGGCAA 
GGTCCAGACT GCTCTCTCTC CGTCCCCTCC ACTGAGGCCT TCTGGGTTCT ACCCAGCATC 
AAACCATCGG CTCAGTCTCT GGGTCGGGCG TCCCACCGGG CCCTGGTGCA TTCTGGGTTG 
ATGTGGGTGG TGGGCGGCTA CTCCTTCAAC TACTCCAACT ACCACATGGT GCTCAACTAC 
AACCTGGAGA CGAGTACGTG GGATGTTGTT CCTGTCAACA ACGGTCCTCA CTACAGATAT 
GGACACTCAC TGGCTCTGTA CCAGGACGAC ATCTACATGT TTGGTGGGAA GCTGGAATCG 
GGATCCGCCA ACGTGACGGA TGAGTTGTGG GTGTTCAACA TCCCCAGACG CACCTGGTCG 
CCACGGAAAC CAGCCCCGCC TCCCCCGTAC GGCCTGGAGG GCCACACGGC CCACGTTGTG 
GAGCTGGCTG ACGGCGAGCC GGTGATGCTC GTCTTTTTTG GATACTCACC GATCTACAGC 
TACATCAACA AGGTCCAGGA GTACAACATC AGGTCCAACT CGTGGCAGGT GGTTTCGGCC 
GGAGGCGCCG TGGTCCAGGG AGGTTACGGC CACAGCAGCG TGTTGGACGA GGCCAGCGGC 
TGCGTGTTCG TCCACGGAGG CTACAAGGCT CTGAGCAACA ACAAATACGG CCTGGTGGAC 
CACATGTACC GATACCACGT CCACACCAAG ACATGGTTGA TCCTCAGGGA GAGCGGCTTG 
GCTCGGTATC TCCACTCGGT GGTGCTGTTG AGCGGCACAC TGCTGGTCTT TGGAGGAAAC 
ACCCACAATG ATACGTCTCT GAGCAACGGA GCCAAGTGTT TTTCAGCCGA CTTTCTGGCG 
TACGATATTG CCTGCGATGA GTGGAGCGTC CTCCCCAGGC CTGGACTGCA CCGAGACATC 
AACCGCTTCG GACACTCTGC TGTGGTCAGC AACGGCTCCA TGTACATCTT TGGAGGTTTT 
TCGGGTCTCC TTCTGAACGA CGTCCTGGCC TACACCCCTC CGTCCTGCCT GGCCTTCTCC 
AGCCAAGCTT CGTGTGCCGC CGCTGGGCCT GGACTTCGCT GCCACTGGGT CAAAAGTAGC 
TGCGTCCCCT GGGAGCCCAA ACCACCGGAC CACGCTTTCC CTGCTCCTTT CTGCCCTGCA 
CAACCCGACA CTACAGACGA ACAGTGTTTC CGGTTCTCAG ACTGTGCGAG CTGCACGGCC 
AACACCAGAG GATGCCAGTG GTGTGAAGAC AGGAAGTGTA TCTCCGCCTC CAGCAACTGC 
ACTGTGCTGA CTCTGGAACT GGCCCAGCAG CATGGCGGCG CTCTGTCTCT GTCTCCACCT 
CCATCCATGT TGTCTGAGCA TCAGCTGAGC TTGTGGAAGC ACGGAGCCAG AAGGAGAGGA 
CATTCGGTGA CAGACTCGTC CAGGTGTAAA CGCAGGGAGG AGCAGGAGTG TTTCCGACTG 
GCCAACTGCA GGAGCTGCTC CCTGAACGCC AACTGTCAGT GGGAGGCGCA GCAGCAGGAG 
TGCCAGGCGC TGCCAGGTAA TCTGTGTGGG GAGGGTTGGC AGCACGTCGG AGAGGCCTGC 
CTGAGGATCA ACTCCAGCAG GGAGAGCTAC GATAACGCCC AGCACTACTG CAAGAACCTG 
GGAGGGAACA TCGCCTCTCT GCTCACTGAC AAACAGGTCA ATTTTGTCCT GGAGGAGCTG 
CAGAAGTATC AGCTGCAGGA CAAGAGGTTA TCTCCATGGG TCGGCCTGAG GAAGATCAAC 
GTGTCGTACT GGGGCTGGGA GGACGCCTCA CCGTTCACCA ACACCAGCCT GAGGTGGTCA 
CCTGGAGAGC CCAGCGACTC TGGATTTTGT GCTTACCTGG AGCGAGCGCA GGTGGCCGGT 
CTGAAGGCAA ACCCCTGCAC GGCCACTACC GACGGCCTGA TCTGTGAAAA GCCTGCCGGG 
AGTCCACAGA GCCAGAGCGC TCGTCCCTGT AAGACGCCGT GCGCGCTACG TAGCAGCTGT 
GCTAACTGCA CCAGCCAGGC CATGGAGTGT ATGTGGTGTG GAAGCACTCA GCGATGCGTC 
GACTCCTCGG CCTACGTCAT CTCCTTTCCC TACGGACAGT GTCTGGAGTG GCAGACCCAG 
GACTGCTCAG CCCAGAACTG TTCCGGTCTA AGGACTTGTT CCGACTGTCT GGAGAGGGTT 
GAGTGCGGCT GGTGTGGAGA TCCCAGTAAC ACCGGCAGAG GTGTCTGCAT GGAGGGCTCC 
TACCGAGGCC CCCTGAAGCC CCTGAACCCG AGGGCCGGGC CCAGAGACAG GGAGAGGCTA 
CGAGACCGGG ACATGGTGGT GGATCAGAAC CTGTGTTCAT CCGATCGAGG CTACAACTGG 
GCATTTATCC AGTGTCCCGC GTGTCAGTGT AACGGCCACA GCAGGTGTGT GAACGGCAGC 
TTGTGTGAAC ACTGCGGGAA CCTGACGGCG GGAACGCACT GCCAGAGCTG CATGCCCGGT 
TACTATGGCG ACCCCACCAA TGGCGGGAAG TGCAACGCGT GCAAGTGCAA CAGTCACGCC 
AACGTCTGCC ACGTCAACAC GGGGAAGTGC TTCTGTACCA CCAAGGGCGT GAAGGGAGAC 
CAGTGTCAGC TATGTGACTC AGAAAACCGT TACCTTGGCA ACCCTCTCCG AGGAACATGT 
TATTACAACC TGTTGATTGA TTACCAGTTT ACCTTCAGTC TGCTGCAGGA AGACGACCGT 
TACTACACCG CCATCAACTT CATGGCCACA CCTGAACAGG CCAACAAAAA CCTGGACATG 
TCCATCAACG CCTCCAACAA CTTCAACCTC AACATCACTT GGTCCTTAAG CTCCACAGCT 
GGAACCATCT CCGGTGAAGA GATGCCCATC ATAGCGAGGA CCAACATCAA GGAGCACAGA 
GACAGTTTCT CCTGTGAGAA GTTCAACTTC AGACTCCATC ACAACATCAC CTTCTACGTC 
TACGTGTCCA ACTTCAGCTG GCCCATCAAA ATCCAGATTG CGTTCTCGCA GCACAACAGC 
ATCATGGACC TGGTGCAGTT CTTCGTTACC TTCTTTAGGT GA