nsf cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis)

Gene
Clones
gRNAs

Summary

Gene Symbol	nsf
Entrez Gene ID	110955464
Full Name	N-ethylmaleimide sensitive factor, vesicle fusing ATPase
Gene Type	protein-coding
Organism	Acanthochromis polyacanthus(spiny chromis)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022200470.1	XP_022056162.1	vesicle-fusing ATPase isoform X1
XM_022200471.1	XP_022056163.1	vesicle-fusing ATPase isoform X2

Pathways from BioSystems
Gene Ontology

The following nsf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nsf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc197151	XM_022200470.1 Latest version!	Acanthochromis polyacanthus N-ethylmaleimide sensitive factor, vesicle fusing ATPase (nsf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OAc197152	XM_022200471.1 Latest version!	Acanthochromis polyacanthus N-ethylmaleimide sensitive factor, vesicle fusing ATPase (nsf), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nsf ( XM_022200470.1 ) cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis) -> XP_022056162.1 Acanthochromis polyacanthus N-ethylmaleimide sensitive factor, vesicle fusing ATPase (nsf), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAc197151
Clone ID Related Accession (Same CDS sequence)	XM_022200470.1
Accession Version	XM_022200470.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-06
Organism	Acanthochromis polyacanthus(spiny chromis)
Product	vesicle-fusing ATPase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019029521.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthochromis polyacanthus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCC GGGTGGTAAT ATTTTTCAAG ACCATGCAAG CGGCGCGATG CCCGACAGAT 
GAGCTGTCGC TGACTAATTG TGCCGTGGTG AGCGAGAAGG ATCTGCAGTC AGGACAACAT 
GTGACTGTGA AGACCACCCC CAACCACAAG TTTGTGTTTA CAGTCAAGAC CCACCACACT 
GTGGCTCCTG GAACCATCGC CTTCAGCCTG CCTCAGAGGA AATGGGCCGG TCTCTCCATT 
GGCCAGGAGG TGGAAGTGGC CAACTACAAC TTCGACAAGT CCAAGCAGTG CATTGGCGCC 
ATGACAATCG AAATCGACTT CCTGCAGAAG AAGAGCACCG ACTCCTCTCC CTACGACTCG 
GACAAGATGG CCGCAGAGTT CATCCAGCAG TTCAACAACC AGGGCTTCTC TGTCACCCAG 
CAGCTAGTGT TTAGTTTCTG CGACAAGCTG TTTGGTTTGA TGGTGAAGGA CATCGAGGCC 
ATGGACGCCA GCATCCTCAA AGGAGAACCA GCCTCCGGAA AGAAACAAAA GATCGACGTC 
GGTCTGATGG TGGGCAACAG CCAGGTGATT TTTGAGAAGG CTGAGAATTC TTCCCTCACA 
CTCGTCGGTA AAGCAAAGAC CAAGGAGGCA CGACAGACAA TCATCAACCC AGACTGGAAC 
TTTGAGAAGA TGGGAATCGG AGGTCTGGAC AAGGAGTTCT CCGACATTTT CCGCAGAGCT 
TTTGCTTCCC GTGTTTTCCC TCCTGATATC GTGGAGCAGA TGGGTTGTAA ACACGTGAAG 
GGCATCTTGC TGTTTGGACC ACCAGGATGT GGTAAAACCC TGATGGCCAG GCAGATCGGT 
AAGATGCTCA ACGCCCGCGA GCCCAAGATT GTCAACGGCC CCGAGATCCT CAACAAGTAC 
GTAGGAGAGT CTGAGGCCAA CATCCGCAAA CTGTTCGCCG AGGCAGAGGA GGAGCAGAAG 
AGGCTGGGAG CCAACAGCGG CCTCCACATC ATCATCTTTG ATGAGTTGGA TGCCATCTGC 
AAGCAGAGAG GAACGGGCGC CAGCAGCACG GGCGTGCACG ACACCGTGGT CAACCAGCTT 
CTGTCCAAGA TCGACGGCGT GGAGCAACTT AACAACATCC TGGTCATTGG TATGACCAAC 
AGGCCTGACC TGATCGATGA CGCCCTGATG AGACCCGGCA GGTTTGAGGT GAAGATGGAA 
ATCGGTCTGC CTGATGAGAA GGGTCGTGTC CAGATCCTGA CCATCCACAC CAACAAGATG 
AGAGGCTTCA ACTTGCTCGC CGTCGATGTC AACATCAAAG AACTGGCATC GGAGACGAAG 
AACTACAGCG GTGCCGAGCT GGAGGGTCTG GTCAGGGCGG CCCAGTCCAC CGCCATGAAC 
CGACACATTA AGGCTACATC TACAGTGGAG GTGGACATGG AGAAGGCAGA GAAGCTGCAG 
GTCACCAGAG CTGACTTCAT GGGTTCCCTC AACAACGACA TAAAACCAGC GTTTGGTACA 
AACCAGGAGG ATTATTCCAG CTATATCATG AACGGCATCA TCAAATGGGG CGACCCGGTC 
ACTCACGTTC TGGATGATGG AGAGCTGCTG GTGCAGCAGA CCAAGAACAG TGACCGCACA 
CCGCTGGTGG CCGTGTTGTT GGAGGGTCCA CCCCACAGTG GAAAGACGGC CCTGGCAGCG 
AAGATTGCAG AGGACTCACA GTTCCCCTTC ATCAAGATCT GTTCTCCAGA CAAGATGATT 
GGACACTCAG AGATCTCCAA GTGCCAGGCA ATCAAAAAGG TCTTCGATGA TGCCTACAAG 
TCTCAGCTCA GCTGTGTGGT TGTGGATGAC ATCGAGCGTC TGCTGGACTA CGTCCCCATC 
GGTCCTCGTT TCTCCAACCT GGTCCTCCAG GCTCTGCTGG TGCTGCTGAA GAAAGCTCCA 
CCCAAGGGTC GGAAGCTGCT CATCATCGGC ACCACCAGCA GGAAGGACGT CCTGCAGGAG 
ATGGAGATGC TGGACGCCTT CAGCACCACC ATCCACATCC CCAACATCTC CACTGGGGAG 
CAGCTAGTCG ACGCCTTAGA GCTGCTGGGG AGCTTCACGG ACAAAGAGCG AGCCAGCATC 
GCACAGCAGC TCAAAGGGAA GCGAGTCTGG ATCGGCATCA AGAAGCTGCT CGTGCTCATC 
GAGATGTCGC TGCAGATGGA CCAGGATTAC AGAGTGACCA AGTTCTTGTC TCTGCTCAGA 
GACGAGGGGG CTGATCGCAG CTTCTACGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022056162.1
CDS	80..2332
Translation	MAARVVIFFKTMQAARCPTDELSLTNCAVVSEKDLQSGQHVTVKTTPNHKFVFTVKTHHTVAPGTIAFSLPQRKWAGLSIGQEVEVANYNFDKSKQCIGAMTIEIDFLQKKSTDSSPYDSDKMAAEFIQQFNNQGFSVTQQLVFSFCDKLFGLMVKDIEAMDASILKGEPASGKKQKIDVGLMVGNSQVIFEKAENSSLTLVGKAKTKEARQTIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLFGPPGCGKTLMARQIGKMLNAREPKIVNGPEILNKYVGESEANIRKLFAEAEEEQKRLGANSGLHIIIFDELDAICKQRGTGASSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDDALMRPGRFEVKMEIGLPDEKGRVQILTIHTNKMRGFNLLAVDVNIKELASETKNYSGAELEGLVRAAQSTAMNRHIKATSTVEVDMEKAEKLQVTRADFMGSLNNDIKPAFGTNQEDYSSYIMNGIIKWGDPVTHVLDDGELLVQQTKNSDRTPLVAVLLEGPPHSGKTALAAKIAEDSQFPFIKICSPDKMIGHSEISKCQAIKKVFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPKGRKLLIIGTTSRKDVLQEMEMLDAFSTTIHIPNISTGEQLVDALELLGSFTDKERASIAQQLKGKRVWIGIKKLLVLIEMSLQMDQDYRVTKFLSLLRDEGADRSFYE

Target ORF information:

RefSeq Version	XM_022200470.1
Organism	Acanthochromis polyacanthus(spiny chromis)
Definition	Acanthochromis polyacanthus N-ethylmaleimide sensitive factor, vesicle fusing ATPase (nsf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022200470.1

ORF Insert Sequence:

ATGGCGGCCC GGGTGGTAAT ATTTTTCAAG ACCATGCAAG CGGCGCGATG CCCGACAGAT 
GAGCTGTCGC TGACTAATTG TGCCGTGGTG AGCGAGAAGG ATCTGCAGTC AGGACAACAT 
GTGACTGTGA AGACCACCCC CAACCACAAG TTTGTGTTTA CAGTCAAGAC CCACCACACT 
GTGGCTCCTG GAACCATCGC CTTCAGCCTG CCTCAGAGGA AATGGGCCGG TCTCTCCATT 
GGCCAGGAGG TGGAAGTGGC CAACTACAAC TTCGACAAGT CCAAGCAGTG CATTGGCGCC 
ATGACAATCG AAATCGACTT CCTGCAGAAG AAGAGCACCG ACTCCTCTCC CTACGACTCG 
GACAAGATGG CCGCAGAGTT CATCCAGCAG TTCAACAACC AGGGCTTCTC TGTCACCCAG 
CAGCTAGTGT TTAGTTTCTG CGACAAGCTG TTTGGTTTGA TGGTGAAGGA CATCGAGGCC 
ATGGACGCCA GCATCCTCAA AGGAGAACCA GCCTCCGGAA AGAAACAAAA GATCGACGTC 
GGTCTGATGG TGGGCAACAG CCAGGTGATT TTTGAGAAGG CTGAGAATTC TTCCCTCACA 
CTCGTCGGTA AAGCAAAGAC CAAGGAGGCA CGACAGACAA TCATCAACCC AGACTGGAAC 
TTTGAGAAGA TGGGAATCGG AGGTCTGGAC AAGGAGTTCT CCGACATTTT CCGCAGAGCT 
TTTGCTTCCC GTGTTTTCCC TCCTGATATC GTGGAGCAGA TGGGTTGTAA ACACGTGAAG 
GGCATCTTGC TGTTTGGACC ACCAGGATGT GGTAAAACCC TGATGGCCAG GCAGATCGGT 
AAGATGCTCA ACGCCCGCGA GCCCAAGATT GTCAACGGCC CCGAGATCCT CAACAAGTAC 
GTAGGAGAGT CTGAGGCCAA CATCCGCAAA CTGTTCGCCG AGGCAGAGGA GGAGCAGAAG 
AGGCTGGGAG CCAACAGCGG CCTCCACATC ATCATCTTTG ATGAGTTGGA TGCCATCTGC 
AAGCAGAGAG GAACGGGCGC CAGCAGCACG GGCGTGCACG ACACCGTGGT CAACCAGCTT 
CTGTCCAAGA TCGACGGCGT GGAGCAACTT AACAACATCC TGGTCATTGG TATGACCAAC 
AGGCCTGACC TGATCGATGA CGCCCTGATG AGACCCGGCA GGTTTGAGGT GAAGATGGAA 
ATCGGTCTGC CTGATGAGAA GGGTCGTGTC CAGATCCTGA CCATCCACAC CAACAAGATG 
AGAGGCTTCA ACTTGCTCGC CGTCGATGTC AACATCAAAG AACTGGCATC GGAGACGAAG 
AACTACAGCG GTGCCGAGCT GGAGGGTCTG GTCAGGGCGG CCCAGTCCAC CGCCATGAAC 
CGACACATTA AGGCTACATC TACAGTGGAG GTGGACATGG AGAAGGCAGA GAAGCTGCAG 
GTCACCAGAG CTGACTTCAT GGGTTCCCTC AACAACGACA TAAAACCAGC GTTTGGTACA 
AACCAGGAGG ATTATTCCAG CTATATCATG AACGGCATCA TCAAATGGGG CGACCCGGTC 
ACTCACGTTC TGGATGATGG AGAGCTGCTG GTGCAGCAGA CCAAGAACAG TGACCGCACA 
CCGCTGGTGG CCGTGTTGTT GGAGGGTCCA CCCCACAGTG GAAAGACGGC CCTGGCAGCG 
AAGATTGCAG AGGACTCACA GTTCCCCTTC ATCAAGATCT GTTCTCCAGA CAAGATGATT 
GGACACTCAG AGATCTCCAA GTGCCAGGCA ATCAAAAAGG TCTTCGATGA TGCCTACAAG 
TCTCAGCTCA GCTGTGTGGT TGTGGATGAC ATCGAGCGTC TGCTGGACTA CGTCCCCATC 
GGTCCTCGTT TCTCCAACCT GGTCCTCCAG GCTCTGCTGG TGCTGCTGAA GAAAGCTCCA 
CCCAAGGGTC GGAAGCTGCT CATCATCGGC ACCACCAGCA GGAAGGACGT CCTGCAGGAG 
ATGGAGATGC TGGACGCCTT CAGCACCACC ATCCACATCC CCAACATCTC CACTGGGGAG 
CAGCTAGTCG ACGCCTTAGA GCTGCTGGGG AGCTTCACGG ACAAAGAGCG AGCCAGCATC 
GCACAGCAGC TCAAAGGGAA GCGAGTCTGG ATCGGCATCA AGAAGCTGCT CGTGCTCATC 
GAGATGTCGC TGCAGATGGA CCAGGATTAC AGAGTGACCA AGTTCTTGTC TCTGCTCAGA 
GACGAGGGGG CTGATCGCAG CTTCTACGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nsf ( XM_022200471.1 ) cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis) -> XP_022056163.1 Acanthochromis polyacanthus N-ethylmaleimide sensitive factor, vesicle fusing ATPase (nsf), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAc197152
Clone ID Related Accession (Same CDS sequence)	XM_022200471.1
Accession Version	XM_022200471.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-06
Organism	Acanthochromis polyacanthus(spiny chromis)
Product	vesicle-fusing ATPase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019029521.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthochromis polyacanthus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCC GGACCATGCA AGCGGCGCGA TGCCCGACAG ATGAGCTGTC GCTGACTAAT 
TGTGCCGTGG TGAGCGAGAA GGATCTGCAG TCAGGACAAC ATGTGACTGT GAAGACCACC 
CCCAACCACA AGTTTGTGTT TACAGTCAAG ACCCACCACA CTGTGGCTCC TGGAACCATC 
GCCTTCAGCC TGCCTCAGAG GAAATGGGCC GGTCTCTCCA TTGGCCAGGA GGTGGAAGTG 
GCCAACTACA ACTTCGACAA GTCCAAGCAG TGCATTGGCG CCATGACAAT CGAAATCGAC 
TTCCTGCAGA AGAAGAGCAC CGACTCCTCT CCCTACGACT CGGACAAGAT GGCCGCAGAG 
TTCATCCAGC AGTTCAACAA CCAGGGCTTC TCTGTCACCC AGCAGCTAGT GTTTAGTTTC 
TGCGACAAGC TGTTTGGTTT GATGGTGAAG GACATCGAGG CCATGGACGC CAGCATCCTC 
AAAGGAGAAC CAGCCTCCGG AAAGAAACAA AAGATCGACG TCGGTCTGAT GGTGGGCAAC 
AGCCAGGTGA TTTTTGAGAA GGCTGAGAAT TCTTCCCTCA CACTCGTCGG TAAAGCAAAG 
ACCAAGGAGG CACGACAGAC AATCATCAAC CCAGACTGGA ACTTTGAGAA GATGGGAATC 
GGAGGTCTGG ACAAGGAGTT CTCCGACATT TTCCGCAGAG CTTTTGCTTC CCGTGTTTTC 
CCTCCTGATA TCGTGGAGCA GATGGGTTGT AAACACGTGA AGGGCATCTT GCTGTTTGGA 
CCACCAGGAT GTGGTAAAAC CCTGATGGCC AGGCAGATCG GTAAGATGCT CAACGCCCGC 
GAGCCCAAGA TTGTCAACGG CCCCGAGATC CTCAACAAGT ACGTAGGAGA GTCTGAGGCC 
AACATCCGCA AACTGTTCGC CGAGGCAGAG GAGGAGCAGA AGAGGCTGGG AGCCAACAGC 
GGCCTCCACA TCATCATCTT TGATGAGTTG GATGCCATCT GCAAGCAGAG AGGAACGGGC 
GCCAGCAGCA CGGGCGTGCA CGACACCGTG GTCAACCAGC TTCTGTCCAA GATCGACGGC 
GTGGAGCAAC TTAACAACAT CCTGGTCATT GGTATGACCA ACAGGCCTGA CCTGATCGAT 
GACGCCCTGA TGAGACCCGG CAGGTTTGAG GTGAAGATGG AAATCGGTCT GCCTGATGAG 
AAGGGTCGTG TCCAGATCCT GACCATCCAC ACCAACAAGA TGAGAGGCTT CAACTTGCTC 
GCCGTCGATG TCAACATCAA AGAACTGGCA TCGGAGACGA AGAACTACAG CGGTGCCGAG 
CTGGAGGGTC TGGTCAGGGC GGCCCAGTCC ACCGCCATGA ACCGACACAT TAAGGCTACA 
TCTACAGTGG AGGTGGACAT GGAGAAGGCA GAGAAGCTGC AGGTCACCAG AGCTGACTTC 
ATGGGTTCCC TCAACAACGA CATAAAACCA GCGTTTGGTA CAAACCAGGA GGATTATTCC 
AGCTATATCA TGAACGGCAT CATCAAATGG GGCGACCCGG TCACTCACGT TCTGGATGAT 
GGAGAGCTGC TGGTGCAGCA GACCAAGAAC AGTGACCGCA CACCGCTGGT GGCCGTGTTG 
TTGGAGGGTC CACCCCACAG TGGAAAGACG GCCCTGGCAG CGAAGATTGC AGAGGACTCA 
CAGTTCCCCT TCATCAAGAT CTGTTCTCCA GACAAGATGA TTGGACACTC AGAGATCTCC 
AAGTGCCAGG CAATCAAAAA GGTCTTCGAT GATGCCTACA AGTCTCAGCT CAGCTGTGTG 
GTTGTGGATG ACATCGAGCG TCTGCTGGAC TACGTCCCCA TCGGTCCTCG TTTCTCCAAC 
CTGGTCCTCC AGGCTCTGCT GGTGCTGCTG AAGAAAGCTC CACCCAAGGG TCGGAAGCTG 
CTCATCATCG GCACCACCAG CAGGAAGGAC GTCCTGCAGG AGATGGAGAT GCTGGACGCC 
TTCAGCACCA CCATCCACAT CCCCAACATC TCCACTGGGG AGCAGCTAGT CGACGCCTTA 
GAGCTGCTGG GGAGCTTCAC GGACAAAGAG CGAGCCAGCA TCGCACAGCA GCTCAAAGGG 
AAGCGAGTCT GGATCGGCAT CAAGAAGCTG CTCGTGCTCA TCGAGATGTC GCTGCAGATG 
GACCAGGATT ACAGAGTGAC CAAGTTCTTG TCTCTGCTCA GAGACGAGGG GGCTGATCGC 
AGCTTCTACG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022056163.1
CDS	92..2326
Translation	MAARTMQAARCPTDELSLTNCAVVSEKDLQSGQHVTVKTTPNHKFVFTVKTHHTVAPGTIAFSLPQRKWAGLSIGQEVEVANYNFDKSKQCIGAMTIEIDFLQKKSTDSSPYDSDKMAAEFIQQFNNQGFSVTQQLVFSFCDKLFGLMVKDIEAMDASILKGEPASGKKQKIDVGLMVGNSQVIFEKAENSSLTLVGKAKTKEARQTIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLFGPPGCGKTLMARQIGKMLNAREPKIVNGPEILNKYVGESEANIRKLFAEAEEEQKRLGANSGLHIIIFDELDAICKQRGTGASSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDDALMRPGRFEVKMEIGLPDEKGRVQILTIHTNKMRGFNLLAVDVNIKELASETKNYSGAELEGLVRAAQSTAMNRHIKATSTVEVDMEKAEKLQVTRADFMGSLNNDIKPAFGTNQEDYSSYIMNGIIKWGDPVTHVLDDGELLVQQTKNSDRTPLVAVLLEGPPHSGKTALAAKIAEDSQFPFIKICSPDKMIGHSEISKCQAIKKVFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPKGRKLLIIGTTSRKDVLQEMEMLDAFSTTIHIPNISTGEQLVDALELLGSFTDKERASIAQQLKGKRVWIGIKKLLVLIEMSLQMDQDYRVTKFLSLLRDEGADRSFYE

Target ORF information:

RefSeq Version	XM_022200471.1
Organism	Acanthochromis polyacanthus(spiny chromis)
Definition	Acanthochromis polyacanthus N-ethylmaleimide sensitive factor, vesicle fusing ATPase (nsf), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022200471.1

ORF Insert Sequence:

ATGGCGGCCC GGACCATGCA AGCGGCGCGA TGCCCGACAG ATGAGCTGTC GCTGACTAAT 
TGTGCCGTGG TGAGCGAGAA GGATCTGCAG TCAGGACAAC ATGTGACTGT GAAGACCACC 
CCCAACCACA AGTTTGTGTT TACAGTCAAG ACCCACCACA CTGTGGCTCC TGGAACCATC 
GCCTTCAGCC TGCCTCAGAG GAAATGGGCC GGTCTCTCCA TTGGCCAGGA GGTGGAAGTG 
GCCAACTACA ACTTCGACAA GTCCAAGCAG TGCATTGGCG CCATGACAAT CGAAATCGAC 
TTCCTGCAGA AGAAGAGCAC CGACTCCTCT CCCTACGACT CGGACAAGAT GGCCGCAGAG 
TTCATCCAGC AGTTCAACAA CCAGGGCTTC TCTGTCACCC AGCAGCTAGT GTTTAGTTTC 
TGCGACAAGC TGTTTGGTTT GATGGTGAAG GACATCGAGG CCATGGACGC CAGCATCCTC 
AAAGGAGAAC CAGCCTCCGG AAAGAAACAA AAGATCGACG TCGGTCTGAT GGTGGGCAAC 
AGCCAGGTGA TTTTTGAGAA GGCTGAGAAT TCTTCCCTCA CACTCGTCGG TAAAGCAAAG 
ACCAAGGAGG CACGACAGAC AATCATCAAC CCAGACTGGA ACTTTGAGAA GATGGGAATC 
GGAGGTCTGG ACAAGGAGTT CTCCGACATT TTCCGCAGAG CTTTTGCTTC CCGTGTTTTC 
CCTCCTGATA TCGTGGAGCA GATGGGTTGT AAACACGTGA AGGGCATCTT GCTGTTTGGA 
CCACCAGGAT GTGGTAAAAC CCTGATGGCC AGGCAGATCG GTAAGATGCT CAACGCCCGC 
GAGCCCAAGA TTGTCAACGG CCCCGAGATC CTCAACAAGT ACGTAGGAGA GTCTGAGGCC 
AACATCCGCA AACTGTTCGC CGAGGCAGAG GAGGAGCAGA AGAGGCTGGG AGCCAACAGC 
GGCCTCCACA TCATCATCTT TGATGAGTTG GATGCCATCT GCAAGCAGAG AGGAACGGGC 
GCCAGCAGCA CGGGCGTGCA CGACACCGTG GTCAACCAGC TTCTGTCCAA GATCGACGGC 
GTGGAGCAAC TTAACAACAT CCTGGTCATT GGTATGACCA ACAGGCCTGA CCTGATCGAT 
GACGCCCTGA TGAGACCCGG CAGGTTTGAG GTGAAGATGG AAATCGGTCT GCCTGATGAG 
AAGGGTCGTG TCCAGATCCT GACCATCCAC ACCAACAAGA TGAGAGGCTT CAACTTGCTC 
GCCGTCGATG TCAACATCAA AGAACTGGCA TCGGAGACGA AGAACTACAG CGGTGCCGAG 
CTGGAGGGTC TGGTCAGGGC GGCCCAGTCC ACCGCCATGA ACCGACACAT TAAGGCTACA 
TCTACAGTGG AGGTGGACAT GGAGAAGGCA GAGAAGCTGC AGGTCACCAG AGCTGACTTC 
ATGGGTTCCC TCAACAACGA CATAAAACCA GCGTTTGGTA CAAACCAGGA GGATTATTCC 
AGCTATATCA TGAACGGCAT CATCAAATGG GGCGACCCGG TCACTCACGT TCTGGATGAT 
GGAGAGCTGC TGGTGCAGCA GACCAAGAAC AGTGACCGCA CACCGCTGGT GGCCGTGTTG 
TTGGAGGGTC CACCCCACAG TGGAAAGACG GCCCTGGCAG CGAAGATTGC AGAGGACTCA 
CAGTTCCCCT TCATCAAGAT CTGTTCTCCA GACAAGATGA TTGGACACTC AGAGATCTCC 
AAGTGCCAGG CAATCAAAAA GGTCTTCGAT GATGCCTACA AGTCTCAGCT CAGCTGTGTG 
GTTGTGGATG ACATCGAGCG TCTGCTGGAC TACGTCCCCA TCGGTCCTCG TTTCTCCAAC 
CTGGTCCTCC AGGCTCTGCT GGTGCTGCTG AAGAAAGCTC CACCCAAGGG TCGGAAGCTG 
CTCATCATCG GCACCACCAG CAGGAAGGAC GTCCTGCAGG AGATGGAGAT GCTGGACGCC 
TTCAGCACCA CCATCCACAT CCCCAACATC TCCACTGGGG AGCAGCTAGT CGACGCCTTA 
GAGCTGCTGG GGAGCTTCAC GGACAAAGAG CGAGCCAGCA TCGCACAGCA GCTCAAAGGG 
AAGCGAGTCT GGATCGGCAT CAAGAAGCTG CTCGTGCTCA TCGAGATGTC GCTGCAGATG 
GACCAGGATT ACAGAGTGAC CAAGTTCTTG TCTCTGCTCA GAGACGAGGG GGCTGATCGC 
AGCTTCTACG AGTAG