LOC110953218 cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC110953218
Entrez Gene ID	110953218
Full Name	alpha-actinin-1
Gene Type	protein-coding
Organism	Acanthochromis polyacanthus(spiny chromis)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022197096.1	XP_022052788.1	alpha-actinin-1 isoform X4
XM_022197093.1	XP_022052785.1	alpha-actinin-1 isoform X1
XM_022197094.1	XP_022052786.1	alpha-actinin-1 isoform X2
XM_022197095.1	XP_022052787.1	alpha-actinin-1 isoform X3
XM_022197097.1	XP_022052789.1	alpha-actinin-1 isoform X5

Pathways from BioSystems
Gene Ontology

The following LOC110953218 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC110953218 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAc194433	XM_022197093.1 Latest version!	Acanthochromis polyacanthus alpha-actinin-1 (LOC110953218), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAc194434	XM_022197094.1 Latest version!	Acanthochromis polyacanthus alpha-actinin-1 (LOC110953218), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAc194435	XM_022197095.1 Latest version!	Acanthochromis polyacanthus alpha-actinin-1 (LOC110953218), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAc194436	XM_022197096.1 Latest version!	Acanthochromis polyacanthus alpha-actinin-1 (LOC110953218), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAc194437	XM_022197097.1 Latest version!	Acanthochromis polyacanthus alpha-actinin-1 (LOC110953218), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC110953218 ( XM_022197093.1 ) cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis) -> XP_022052785.1 Acanthochromis polyacanthus alpha-actinin-1 (LOC110953218), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAc194433
Clone ID Related Accession (Same CDS sequence)	XM_022197093.1
Accession Version	XM_022197093.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-06
Organism	Acanthochromis polyacanthus(spiny chromis)
Product	alpha-actinin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019029399.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthochromis polyacanthus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATAATA TGGATCAGTA TGATGGAGAG AATGACTACA TGCAGCAGGA GGACGACTGG 
GACCGGGATC TGCTCCTGGA TCCTGCATGG GAGAAGCAGC AGAGAAAGAC ATTTACAGCC 
TGGTGTAACT CTCACCTGAG GAAAGCAGGA ACGCAGATCG AGAACATTGA GGAGGACTTC 
CGGGACGGCC TGAAACTCAT GCTGCTGCTG GAGGTCATCT CAGGTGAACG CTTGGCCAAA 
CCAGAAAGAG GCAAAATGAG AGTCCACAAG ATCTCCAACG TTAACAAAGC GCTCGACTTC 
ATCGCCAGCA AAGGAGTAAA GCTGGTTTCT ATTGGAGCAG AAGAGATCGT AGACGGCAAC 
GCTAAGATGA CCCTGGGAAT GATCTGGACT ATAATCCTCC GCTTCGCCAT CCAAGACATT 
TCTGTGGAAG AGACATCTGC CAAAGAGGGC CTCCTGCTGT GGTGCCAGAG GAAGACTGCG 
CCCTACAAGA ATGTCAACAT CCAGAACTTT CACATCAGCT GGAAAGACGG TTTGGGTTTC 
TGTGCACTCA TTCATCGACA CCGTCCAGAA CTCATCGACT ACGGAAAACT GCGCAAGGAC 
GACCCGATGA CCAACCTGAA CACAGCCTTC GACGTGGCGG AGAAGTACCT GGACATCCCC 
AAGATGTTGG ACGCAGAAGA CATTGTGGGC ACTGCCCGTC CGGACGAGAA GGCCATCATG 
ACCTACGTCT CTAGCTTCTA CCACGCCTTC TCAGGCGCCC AGAAGGCAGA GACGGCAGCC 
AACAGGATCT GCAAGGTGCT GGCTGTCAAC CAAGAGAATG AGCAGCTGAT GGAGGACTAT 
GAGAAGCTGG CCAGCGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC 
CGATTGCCCG AGAACACCAT GCAGGCCATG CAGCAGAAGC TGGAGGACTT CAGAGATTAC 
CGCCGCCTCC ACAAGCCGCC CAAAGTGCAG GAGAAGTGCC AGCTGGAGAT CAACTTCAAC 
ACCCTGCAGA CNAAGCTGAG GCTCAGCAAC AGGCCCGCCT TCATGCCCTC GGAGGGAAAG 
ATGGTCTCGG ACATTAACAA TGCATGGGGG AGCCTAGAGG GAGCAGAGAA GGGCTATGAA 
GAGTGGCTCC TCAATGAGAT TCGCCGGCTG GAAAGACTCG ATCACCTGGC AGAGAAATTC 
CGCCAGAAAG CAGCGATCCA TGAAGCCTGG ACTGAAGGTA AAGAAGAAAT GCTGCAGAAA 
CGGGACTACG AGACAGCCTC GCTGTCAGAT ATCAAGGCCT TGCTGAAGAA ACACGAGGCC 
TTCGAGAGCG ATCTGGCTGC ACACCAGGAC CGTGTGGAGC AGATTGCAGC CATCGCACAG 
GAGTTAAATG AGCTGGACTA CTACGACTCC CCCAGTGTAA ACGCTCGCTG CCAGCGGATC 
TGCGACCAAT GGGACGCGCT GGGAGCTCTG ACCCAGAAAC GCAGCGAGGC TTTGCAGAGA 
ACCGAGAAGC TGCTTGAGAC CATCGACCAA CTGTACCTTG AGTTTGCCAA AAGAGCGGCT 
CCGTTCAACA ACTGGATGGA GGGAGCCATG GAGGACCTGC AGGACACTTT TATCGTCCAC 
ACCATCGAGG AAATCCAGGG ATTGAGCACG GCCCACGAGC AGTTCAAAGC TACACTGCCA 
GAGGCCGACA AAGAGCGTCT GGCCATCCTG GGCATCCACA ACGAGATCGC CAAAATCGTG 
CAGACCTACC ACGTGAACAT GGCCGGAACC AACCCGTACA CCACCATCAA CCCACAGGAG 
ATCAACGCCA AATGGGACAA GGTCCGACAG CTGGTTCCTC AGCGCGACCA GGCTCTGATT 
GAGGAACACG CTCGGCAGCA GAACAACGAA CGTCTGCGCC GACAGTTCGC CAACCAGGCC 
AACGTCATCG GGCCCTGGAT CCAGAACAAG ATGGAGGAAA TCGGTCGTAT CTCGATTGAG 
ATGCACGGCA CCCTGGAGGA TCAGCTGACG CACCTCCGCC AGTACGAGAA AAGCATCGTC 
AACTACAAGC CAAAGATCGA CCAGCTGGAG GGAGACCACC AGCTCATTCA GGAAGCCCTC 
ATCTTCGACA ACAAGCACAC CAACTACACC ATGGAGCACA TCCGTGTGGG CTGGGAGCAG 
CTGCTCACCA CCATCGCCCG AACCATCAAC GAGATCGAGA ACCAGATCTT GACGAGAGAC 
GCCAAAGGCA TCAGCCAGGA GCAGCTGAAC GAGTTCCGGG CCTCCTTCAA CCACTTCGAC 
AGGGACCACT CGGGCACTCT GGGCGCAGAG GAGTTCAAGG CCTGCCTGAT CAGCCTGGGC 
TTCGATATCG CCAACGATGC ACAGAAGAGA ACAGGAATCA TGGATGCTGA AGACTTCAAA 
ACCTGCCTTA TCTCCGTGGG TTACAACCTG GGTGAAAACG AGTTCTCCCG CATCATGAGC 
ATAGTGGATC CCAACAGGAT GGGCATCGTC ACCTTCCAGG CCTTCATCGA CTTCATGTCC 
CGCGAGACGG CGGACACGGA CACCGCCGAC CAGGTCATGG CCTCCTTCAA GGTCCTGGCG 
GGCGATAAGA ACTACATCTT AGCCGACGAG CTTCGCCGTG AGCTTCCTCC AGACCAGGCT 
GATTACTGCA TCGCCCGCAT GGCGCCCTAC TGCGGCCCGG ACGCCGTTCC CGGAGCCCTC 
GACTACATGT CCTTCTCCAC GGCTCTGTAC GGGGAGAGCG ACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022052785.1
CDS	267..3014
Translation	MYNMDQYDGENDYMQQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPMTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRLPENTMQAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQnullKLRLSNRPAFMPSEGKMVSDINNAWGSLEGAEKGYEEWLLNEIRRLERLDHLAEKFRQKAAIHEAWTEGKEEMLQKRDYETASLSDIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQRICDQWDALGALTQKRSEALQRTEKLLETIDQLYLEFAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLSTAHEQFKATLPEADKERLAILGIHNEIAKIVQTYHVNMAGTNPYTTINPQEINAKWDKVRQLVPQRDQALIEEHARQQNNERLRRQFANQANVIGPWIQNKMEEIGRISIEMHGTLEDQLTHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEIENQILTRDAKGISQEQLNEFRASFNHFDRDHSGTLGAEEFKACLISLGFDIANDAQKRTGIMDAEDFKTCLISVGYNLGENEFSRIMSIVDPNRMGIVTFQAFIDFMSRETADTDTADQVMASFKVLAGDKNYILADELRRELPPDQADYCIARMAPYCGPDAVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_022197093.1
Organism	Acanthochromis polyacanthus(spiny chromis)
Definition	Acanthochromis polyacanthus alpha-actinin-1 (LOC110953218), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022197093.1

ORF Insert Sequence:

ATGTATAATA TGGATCAGTA TGATGGAGAG AATGACTACA TGCAGCAGGA GGACGACTGG 
GACCGGGATC TGCTCCTGGA TCCTGCATGG GAGAAGCAGC AGAGAAAGAC ATTTACAGCC 
TGGTGTAACT CTCACCTGAG GAAAGCAGGA ACGCAGATCG AGAACATTGA GGAGGACTTC 
CGGGACGGCC TGAAACTCAT GCTGCTGCTG GAGGTCATCT CAGGTGAACG CTTGGCCAAA 
CCAGAAAGAG GCAAAATGAG AGTCCACAAG ATCTCCAACG TTAACAAAGC GCTCGACTTC 
ATCGCCAGCA AAGGAGTAAA GCTGGTTTCT ATTGGAGCAG AAGAGATCGT AGACGGCAAC 
GCTAAGATGA CCCTGGGAAT GATCTGGACT ATAATCCTCC GCTTCGCCAT CCAAGACATT 
TCTGTGGAAG AGACATCTGC CAAAGAGGGC CTCCTGCTGT GGTGCCAGAG GAAGACTGCG 
CCCTACAAGA ATGTCAACAT CCAGAACTTT CACATCAGCT GGAAAGACGG TTTGGGTTTC 
TGTGCACTCA TTCATCGACA CCGTCCAGAA CTCATCGACT ACGGAAAACT GCGCAAGGAC 
GACCCGATGA CCAACCTGAA CACAGCCTTC GACGTGGCGG AGAAGTACCT GGACATCCCC 
AAGATGTTGG ACGCAGAAGA CATTGTGGGC ACTGCCCGTC CGGACGAGAA GGCCATCATG 
ACCTACGTCT CTAGCTTCTA CCACGCCTTC TCAGGCGCCC AGAAGGCAGA GACGGCAGCC 
AACAGGATCT GCAAGGTGCT GGCTGTCAAC CAAGAGAATG AGCAGCTGAT GGAGGACTAT 
GAGAAGCTGG CCAGCGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC 
CGATTGCCCG AGAACACCAT GCAGGCCATG CAGCAGAAGC TGGAGGACTT CAGAGATTAC 
CGCCGCCTCC ACAAGCCGCC CAAAGTGCAG GAGAAGTGCC AGCTGGAGAT CAACTTCAAC 
ACCCTGCAGA CNAAGCTGAG GCTCAGCAAC AGGCCCGCCT TCATGCCCTC GGAGGGAAAG 
ATGGTCTCGG ACATTAACAA TGCATGGGGG AGCCTAGAGG GAGCAGAGAA GGGCTATGAA 
GAGTGGCTCC TCAATGAGAT TCGCCGGCTG GAAAGACTCG ATCACCTGGC AGAGAAATTC 
CGCCAGAAAG CAGCGATCCA TGAAGCCTGG ACTGAAGGTA AAGAAGAAAT GCTGCAGAAA 
CGGGACTACG AGACAGCCTC GCTGTCAGAT ATCAAGGCCT TGCTGAAGAA ACACGAGGCC 
TTCGAGAGCG ATCTGGCTGC ACACCAGGAC CGTGTGGAGC AGATTGCAGC CATCGCACAG 
GAGTTAAATG AGCTGGACTA CTACGACTCC CCCAGTGTAA ACGCTCGCTG CCAGCGGATC 
TGCGACCAAT GGGACGCGCT GGGAGCTCTG ACCCAGAAAC GCAGCGAGGC TTTGCAGAGA 
ACCGAGAAGC TGCTTGAGAC CATCGACCAA CTGTACCTTG AGTTTGCCAA AAGAGCGGCT 
CCGTTCAACA ACTGGATGGA GGGAGCCATG GAGGACCTGC AGGACACTTT TATCGTCCAC 
ACCATCGAGG AAATCCAGGG ATTGAGCACG GCCCACGAGC AGTTCAAAGC TACACTGCCA 
GAGGCCGACA AAGAGCGTCT GGCCATCCTG GGCATCCACA ACGAGATCGC CAAAATCGTG 
CAGACCTACC ACGTGAACAT GGCCGGAACC AACCCGTACA CCACCATCAA CCCACAGGAG 
ATCAACGCCA AATGGGACAA GGTCCGACAG CTGGTTCCTC AGCGCGACCA GGCTCTGATT 
GAGGAACACG CTCGGCAGCA GAACAACGAA CGTCTGCGCC GACAGTTCGC CAACCAGGCC 
AACGTCATCG GGCCCTGGAT CCAGAACAAG ATGGAGGAAA TCGGTCGTAT CTCGATTGAG 
ATGCACGGCA CCCTGGAGGA TCAGCTGACG CACCTCCGCC AGTACGAGAA AAGCATCGTC 
AACTACAAGC CAAAGATCGA CCAGCTGGAG GGAGACCACC AGCTCATTCA GGAAGCCCTC 
ATCTTCGACA ACAAGCACAC CAACTACACC ATGGAGCACA TCCGTGTGGG CTGGGAGCAG 
CTGCTCACCA CCATCGCCCG AACCATCAAC GAGATCGAGA ACCAGATCTT GACGAGAGAC 
GCCAAAGGCA TCAGCCAGGA GCAGCTGAAC GAGTTCCGGG CCTCCTTCAA CCACTTCGAC 
AGGGACCACT CGGGCACTCT GGGCGCAGAG GAGTTCAAGG CCTGCCTGAT CAGCCTGGGC 
TTCGATATCG CCAACGATGC ACAGAAGAGA ACAGGAATCA TGGATGCTGA AGACTTCAAA 
ACCTGCCTTA TCTCCGTGGG TTACAACCTG GGTGAAAACG AGTTCTCCCG CATCATGAGC 
ATAGTGGATC CCAACAGGAT GGGCATCGTC ACCTTCCAGG CCTTCATCGA CTTCATGTCC 
CGCGAGACGG CGGACACGGA CACCGCCGAC CAGGTCATGG CCTCCTTCAA GGTCCTGGCG 
GGCGATAAGA ACTACATCTT AGCCGACGAG CTTCGCCGTG AGCTTCCTCC AGACCAGGCT 
GATTACTGCA TCGCCCGCAT GGCGCCCTAC TGCGGCCCGG ACGCCGTTCC CGGAGCCCTC 
GACTACATGT CCTTCTCCAC GGCTCTGTAC GGGGAGAGCG ACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110953218 ( XM_022197094.1 ) cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis) -> XP_022052786.1 Acanthochromis polyacanthus alpha-actinin-1 (LOC110953218), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAc194434
Clone ID Related Accession (Same CDS sequence)	XM_022197094.1
Accession Version	XM_022197094.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-06
Organism	Acanthochromis polyacanthus(spiny chromis)
Product	alpha-actinin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019029399.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthochromis polyacanthus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATAATA TGGATCAGTA TGATGGAGAG AATGACTACA TGCAGCAGGA GGACGACTGG 
GACCGGGATC TGCTCCTGGA TCCTGCATGG GAGAAGCAGC AGAGAAAGAC ATTTACAGCC 
TGGTGTAACT CTCACCTGAG GAAAGCAGGA ACGCAGATCG AGAACATTGA GGAGGACTTC 
CGGGACGGCC TGAAACTCAT GCTGCTGCTG GAGGTCATCT CAGGTGAACG CTTGGCCAAA 
CCAGAAAGAG GCAAAATGAG AGTCCACAAG ATCTCCAACG TTAACAAAGC GCTCGACTTC 
ATCGCCAGCA AAGGAGTAAA GCTGGTTTCT ATTGGAGCAG AAGAGATCGT AGACGGCAAC 
GCTAAGATGA CCCTGGGAAT GATCTGGACT ATAATCCTCC GCTTCGCCAT CCAAGACATT 
TCTGTGGAAG AGACATCTGC CAAAGAGGGC CTCCTGCTGT GGTGCCAGAG GAAGACTGCG 
CCCTACAAGA ATGTCAACAT CCAGAACTTT CACATCAGCT GGAAAGACGG TTTGGGTTTC 
TGTGCACTCA TTCATCGACA CCGTCCAGAA CTCATCGACT ACGGAAAACT GCGCAAGGAC 
GACCCGATGA CCAACCTGAA CACAGCCTTC GACGTGGCGG AGAAGTACCT GGACATCCCC 
AAGATGTTGG ACGCAGAAGA TATCATTAGC ACCTTGAGGC CCGATGAGAA GGCCGTCATG 
ACTTATGTGT CGTGTTACTA CCACGCGTTC TCAGGCAAGC AGAAGGCAGA GACGGCAGCC 
AACAGGATCT GCAAGGTGCT GGCTGTCAAC CAAGAGAATG AGCAGCTGAT GGAGGACTAT 
GAGAAGCTGG CCAGCGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC 
CGATTGCCCG AGAACACCAT GCAGGCCATG CAGCAGAAGC TGGAGGACTT CAGAGATTAC 
CGCCGCCTCC ACAAGCCGCC CAAAGTGCAG GAGAAGTGCC AGCTGGAGAT CAACTTCAAC 
ACCCTGCAGA CNAAGCTGAG GCTCAGCAAC AGGCCCGCCT TCATGCCCTC GGAGGGAAAG 
ATGGTCTCGG ACATTAACAA TGCATGGGGG AGCCTAGAGG GAGCAGAGAA GGGCTATGAA 
GAGTGGCTCC TCAATGAGAT TCGCCGGCTG GAAAGACTCG ATCACCTGGC AGAGAAATTC 
CGCCAGAAAG CAGCGATCCA TGAAGCCTGG ACTGAAGGTA AAGAAGAAAT GCTGCAGAAA 
CGGGACTACG AGACAGCCTC GCTGTCAGAT ATCAAGGCCT TGCTGAAGAA ACACGAGGCC 
TTCGAGAGCG ATCTGGCTGC ACACCAGGAC CGTGTGGAGC AGATTGCAGC CATCGCACAG 
GAGTTAAATG AGCTGGACTA CTACGACTCC CCCAGTGTAA ACGCTCGCTG CCAGCGGATC 
TGCGACCAAT GGGACGCGCT GGGAGCTCTG ACCCAGAAAC GCAGCGAGGC TTTGCAGAGA 
ACCGAGAAGC TGCTTGAGAC CATCGACCAA CTGTACCTTG AGTTTGCCAA AAGAGCGGCT 
CCGTTCAACA ACTGGATGGA GGGAGCCATG GAGGACCTGC AGGACACTTT TATCGTCCAC 
ACCATCGAGG AAATCCAGGG ATTGAGCACG GCCCACGAGC AGTTCAAAGC TACACTGCCA 
GAGGCCGACA AAGAGCGTCT GGCCATCCTG GGCATCCACA ACGAGATCGC CAAAATCGTG 
CAGACCTACC ACGTGAACAT GGCCGGAACC AACCCGTACA CCACCATCAA CCCACAGGAG 
ATCAACGCCA AATGGGACAA GGTCCGACAG CTGGTTCCTC AGCGCGACCA GGCTCTGATT 
GAGGAACACG CTCGGCAGCA GAACAACGAA CGTCTGCGCC GACAGTTCGC CAACCAGGCC 
AACGTCATCG GGCCCTGGAT CCAGAACAAG ATGGAGGAAA TCGGTCGTAT CTCGATTGAG 
ATGCACGGCA CCCTGGAGGA TCAGCTGACG CACCTCCGCC AGTACGAGAA AAGCATCGTC 
AACTACAAGC CAAAGATCGA CCAGCTGGAG GGAGACCACC AGCTCATTCA GGAAGCCCTC 
ATCTTCGACA ACAAGCACAC CAACTACACC ATGGAGCACA TCCGTGTGGG CTGGGAGCAG 
CTGCTCACCA CCATCGCCCG AACCATCAAC GAGATCGAGA ACCAGATCTT GACGAGAGAC 
GCCAAAGGCA TCAGCCAGGA GCAGCTGAAC GAGTTCCGGG CCTCCTTCAA CCACTTCGAC 
AGGGACCACT CGGGCACTCT GGGCGCAGAG GAGTTCAAGG CCTGCCTGAT CAGCCTGGGC 
TTCGATATCG CCAACGATGC ACAGAAGAGA ACAGGAATCA TGGATGCTGA AGACTTCAAA 
ACCTGCCTTA TCTCCGTGGG TTACAACCTG GGTGAAAACG AGTTCTCCCG CATCATGAGC 
ATAGTGGATC CCAACAGGAT GGGCATCGTC ACCTTCCAGG CCTTCATCGA CTTCATGTCC 
CGCGAGACGG CGGACACGGA CACCGCCGAC CAGGTCATGG CCTCCTTCAA GGTCCTGGCG 
GGCGATAAGA ACTACATCTT AGCCGACGAG CTTCGCCGTG AGCTTCCTCC AGACCAGGCT 
GATTACTGCA TCGCCCGCAT GGCGCCCTAC TGCGGCCCGG ACGCCGTTCC CGGAGCCCTC 
GACTACATGT CCTTCTCCAC GGCTCTGTAC GGGGAGAGCG ACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022052786.1
CDS	267..3014
Translation	MYNMDQYDGENDYMQQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPMTNLNTAFDVAEKYLDIPKMLDAEDIISTLRPDEKAVMTYVSCYYHAFSGKQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRLPENTMQAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQnullKLRLSNRPAFMPSEGKMVSDINNAWGSLEGAEKGYEEWLLNEIRRLERLDHLAEKFRQKAAIHEAWTEGKEEMLQKRDYETASLSDIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQRICDQWDALGALTQKRSEALQRTEKLLETIDQLYLEFAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLSTAHEQFKATLPEADKERLAILGIHNEIAKIVQTYHVNMAGTNPYTTINPQEINAKWDKVRQLVPQRDQALIEEHARQQNNERLRRQFANQANVIGPWIQNKMEEIGRISIEMHGTLEDQLTHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEIENQILTRDAKGISQEQLNEFRASFNHFDRDHSGTLGAEEFKACLISLGFDIANDAQKRTGIMDAEDFKTCLISVGYNLGENEFSRIMSIVDPNRMGIVTFQAFIDFMSRETADTDTADQVMASFKVLAGDKNYILADELRRELPPDQADYCIARMAPYCGPDAVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_022197094.1
Organism	Acanthochromis polyacanthus(spiny chromis)
Definition	Acanthochromis polyacanthus alpha-actinin-1 (LOC110953218), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022197094.1

ORF Insert Sequence:

ATGTATAATA TGGATCAGTA TGATGGAGAG AATGACTACA TGCAGCAGGA GGACGACTGG 
GACCGGGATC TGCTCCTGGA TCCTGCATGG GAGAAGCAGC AGAGAAAGAC ATTTACAGCC 
TGGTGTAACT CTCACCTGAG GAAAGCAGGA ACGCAGATCG AGAACATTGA GGAGGACTTC 
CGGGACGGCC TGAAACTCAT GCTGCTGCTG GAGGTCATCT CAGGTGAACG CTTGGCCAAA 
CCAGAAAGAG GCAAAATGAG AGTCCACAAG ATCTCCAACG TTAACAAAGC GCTCGACTTC 
ATCGCCAGCA AAGGAGTAAA GCTGGTTTCT ATTGGAGCAG AAGAGATCGT AGACGGCAAC 
GCTAAGATGA CCCTGGGAAT GATCTGGACT ATAATCCTCC GCTTCGCCAT CCAAGACATT 
TCTGTGGAAG AGACATCTGC CAAAGAGGGC CTCCTGCTGT GGTGCCAGAG GAAGACTGCG 
CCCTACAAGA ATGTCAACAT CCAGAACTTT CACATCAGCT GGAAAGACGG TTTGGGTTTC 
TGTGCACTCA TTCATCGACA CCGTCCAGAA CTCATCGACT ACGGAAAACT GCGCAAGGAC 
GACCCGATGA CCAACCTGAA CACAGCCTTC GACGTGGCGG AGAAGTACCT GGACATCCCC 
AAGATGTTGG ACGCAGAAGA TATCATTAGC ACCTTGAGGC CCGATGAGAA GGCCGTCATG 
ACTTATGTGT CGTGTTACTA CCACGCGTTC TCAGGCAAGC AGAAGGCAGA GACGGCAGCC 
AACAGGATCT GCAAGGTGCT GGCTGTCAAC CAAGAGAATG AGCAGCTGAT GGAGGACTAT 
GAGAAGCTGG CCAGCGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC 
CGATTGCCCG AGAACACCAT GCAGGCCATG CAGCAGAAGC TGGAGGACTT CAGAGATTAC 
CGCCGCCTCC ACAAGCCGCC CAAAGTGCAG GAGAAGTGCC AGCTGGAGAT CAACTTCAAC 
ACCCTGCAGA CNAAGCTGAG GCTCAGCAAC AGGCCCGCCT TCATGCCCTC GGAGGGAAAG 
ATGGTCTCGG ACATTAACAA TGCATGGGGG AGCCTAGAGG GAGCAGAGAA GGGCTATGAA 
GAGTGGCTCC TCAATGAGAT TCGCCGGCTG GAAAGACTCG ATCACCTGGC AGAGAAATTC 
CGCCAGAAAG CAGCGATCCA TGAAGCCTGG ACTGAAGGTA AAGAAGAAAT GCTGCAGAAA 
CGGGACTACG AGACAGCCTC GCTGTCAGAT ATCAAGGCCT TGCTGAAGAA ACACGAGGCC 
TTCGAGAGCG ATCTGGCTGC ACACCAGGAC CGTGTGGAGC AGATTGCAGC CATCGCACAG 
GAGTTAAATG AGCTGGACTA CTACGACTCC CCCAGTGTAA ACGCTCGCTG CCAGCGGATC 
TGCGACCAAT GGGACGCGCT GGGAGCTCTG ACCCAGAAAC GCAGCGAGGC TTTGCAGAGA 
ACCGAGAAGC TGCTTGAGAC CATCGACCAA CTGTACCTTG AGTTTGCCAA AAGAGCGGCT 
CCGTTCAACA ACTGGATGGA GGGAGCCATG GAGGACCTGC AGGACACTTT TATCGTCCAC 
ACCATCGAGG AAATCCAGGG ATTGAGCACG GCCCACGAGC AGTTCAAAGC TACACTGCCA 
GAGGCCGACA AAGAGCGTCT GGCCATCCTG GGCATCCACA ACGAGATCGC CAAAATCGTG 
CAGACCTACC ACGTGAACAT GGCCGGAACC AACCCGTACA CCACCATCAA CCCACAGGAG 
ATCAACGCCA AATGGGACAA GGTCCGACAG CTGGTTCCTC AGCGCGACCA GGCTCTGATT 
GAGGAACACG CTCGGCAGCA GAACAACGAA CGTCTGCGCC GACAGTTCGC CAACCAGGCC 
AACGTCATCG GGCCCTGGAT CCAGAACAAG ATGGAGGAAA TCGGTCGTAT CTCGATTGAG 
ATGCACGGCA CCCTGGAGGA TCAGCTGACG CACCTCCGCC AGTACGAGAA AAGCATCGTC 
AACTACAAGC CAAAGATCGA CCAGCTGGAG GGAGACCACC AGCTCATTCA GGAAGCCCTC 
ATCTTCGACA ACAAGCACAC CAACTACACC ATGGAGCACA TCCGTGTGGG CTGGGAGCAG 
CTGCTCACCA CCATCGCCCG AACCATCAAC GAGATCGAGA ACCAGATCTT GACGAGAGAC 
GCCAAAGGCA TCAGCCAGGA GCAGCTGAAC GAGTTCCGGG CCTCCTTCAA CCACTTCGAC 
AGGGACCACT CGGGCACTCT GGGCGCAGAG GAGTTCAAGG CCTGCCTGAT CAGCCTGGGC 
TTCGATATCG CCAACGATGC ACAGAAGAGA ACAGGAATCA TGGATGCTGA AGACTTCAAA 
ACCTGCCTTA TCTCCGTGGG TTACAACCTG GGTGAAAACG AGTTCTCCCG CATCATGAGC 
ATAGTGGATC CCAACAGGAT GGGCATCGTC ACCTTCCAGG CCTTCATCGA CTTCATGTCC 
CGCGAGACGG CGGACACGGA CACCGCCGAC CAGGTCATGG CCTCCTTCAA GGTCCTGGCG 
GGCGATAAGA ACTACATCTT AGCCGACGAG CTTCGCCGTG AGCTTCCTCC AGACCAGGCT 
GATTACTGCA TCGCCCGCAT GGCGCCCTAC TGCGGCCCGG ACGCCGTTCC CGGAGCCCTC 
GACTACATGT CCTTCTCCAC GGCTCTGTAC GGGGAGAGCG ACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110953218 ( XM_022197095.1 ) cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis) -> XP_022052787.1 Acanthochromis polyacanthus alpha-actinin-1 (LOC110953218), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OAc194435
Clone ID Related Accession (Same CDS sequence)	XM_022197095.1
Accession Version	XM_022197095.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-06
Organism	Acanthochromis polyacanthus(spiny chromis)
Product	alpha-actinin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019029399.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthochromis polyacanthus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATAATA TGGATCAGTA TGATGGAGAG AATGACTACA TGCAGCAGGA GGACGACTGG 
GACCGGGATC TGCTCCTGGA TCCTGCATGG GAGAAGCAGC AGAGAAAGAC ATTTACAGCC 
TGGTGTAACT CTCACCTGAG GAAAGCAGGA ACGCAGATCG AGAACATTGA GGAGGACTTC 
CGGGACGGCC TGAAACTCAT GCTGCTGCTG GAGGTCATCT CAGGTGAACG CTTGGCCAAA 
CCAGAAAGAG GCAAAATGAG AGTCCACAAG ATCTCCAACG TTAACAAAGC GCTCGACTTC 
ATCGCCAGCA AAGGAGTAAA GCTGGTTTCT ATTGGAGCAG AAGAGATCGT AGACGGCAAC 
GCTAAGATGA CCCTGGGAAT GATCTGGACT ATAATCCTCC GCTTCGCCAT CCAAGACATT 
TCTGTGGAAG AGACATCTGC CAAAGAGGGC CTCCTGCTGT GGTGCCAGAG GAAGACTGCG 
CCCTACAAGA ATGTCAACAT CCAGAACTTT CACATCAGCT GGAAAGACGG TTTGGGTTTC 
TGTGCACTCA TTCATCGACA CCGTCCAGAA CTCATCGACT ACGGAAAACT GCGCAAGGAC 
GACCCGATGA CCAACCTGAA CACAGCCTTC GACGTGGCGG AGAAGTACCT GGACATCCCC 
AAGATGTTGG ACGCAGAAGA CATTGTGGGC ACTGCCCGTC CGGACGAGAA GGCCATCATG 
ACCTACGTCT CTAGCTTCTA CCACGCCTTC TCAGGCGCCC AGAAGGCAGA GACGGCAGCC 
AACAGGATCT GCAAGGTGCT GGCTGTCAAC CAAGAGAATG AGCAGCTGAT GGAGGACTAT 
GAGAAGCTGG CCAGCGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC 
CGATTGCCCG AGAACACCAT GCAGGCCATG CAGCAGAAGC TGGAGGACTT CAGAGATTAC 
CGCCGCCTCC ACAAGCCGCC CAAAGTGCAG GAGAAGTGCC AGCTGGAGAT CAACTTCAAC 
ACCCTGCAGA CNAAGCTGAG GCTCAGCAAC AGGCCCGCCT TCATGCCCTC GGAGGGAAAG 
ATGGTCTCGG ACATTAACAA TGCATGGGGG AGCCTAGAGG GAGCAGAGAA GGGCTATGAA 
GAGTGGCTCC TCAATGAGAT TCGCCGGCTG GAAAGACTCG ATCACCTGGC AGAGAAATTC 
CGCCAGAAAG CAGCGATCCA TGAAGCCTGG ACTGAAGGTA AAGAAGAAAT GCTGCAGAAA 
CGGGACTACG AGACAGCCTC GCTGTCAGAT ATCAAGGCCT TGCTGAAGAA ACACGAGGCC 
TTCGAGAGCG ATCTGGCTGC ACACCAGGAC CGTGTGGAGC AGATTGCAGC CATCGCACAG 
GAGTTAAATG AGCTGGACTA CTACGACTCC CCCAGTGTAA ACGCTCGCTG CCAGCGGATC 
TGCGACCAAT GGGACGCGCT GGGAGCTCTG ACCCAGAAAC GCAGCGAGGC TTTGCAGAGA 
ACCGAGAAGC TGCTTGAGAC CATCGACCAA CTGTACCTTG AGTTTGCCAA AAGAGCGGCT 
CCGTTCAACA ACTGGATGGA GGGAGCCATG GAGGACCTGC AGGACACTTT TATCGTCCAC 
ACCATCGAGG AAATCCAGGG ATTGAGCACG GCCCACGAGC AGTTCAAAGC TACACTGCCA 
GAGGCCGACA AAGAGCGTCT GGCCATCCTG GGCATCCACA ACGAGATCGC CAAAATCGTG 
CAGACCTACC ACGTGAACAT GGCCGGAACC AACCCGTACA CCACCATCAA CCCACAGGAG 
ATCAACGCCA AATGGGACAA GGTCCGACAG CTGGTTCCTC AGCGCGACCA GGCTCTGATT 
GAGGAACACG CTCGGCAGCA GAACAACGAA CGTCTGCGCC GACAGTTCGC CAACCAGGCC 
AACGTCATCG GGCCCTGGAT CCAGAACAAG ATGGAGGAAA TCGGTCGTAT CTCGATTGAG 
ATGCACGGCA CCCTGGAGGA TCAGCTGACG CACCTCCGCC AGTACGAGAA AAGCATCGTC 
AACTACAAGC CAAAGATCGA CCAGCTGGAG GGAGACCACC AGCTCATTCA GGAAGCCCTC 
ATCTTCGACA ACAAGCACAC CAACTACACC ATGGAGCACA TCCGTGTGGG CTGGGAGCAG 
CTGCTCACCA CCATCGCCCG AACCATCAAC GAGATCGAGA ACCAGATCTT GACGAGAGAC 
GCCAAAGGCA TCAGCCAGGA GCAGCTGAAC GAGTTCCGGG CCTCCTTCAA CCACTTCGAC 
AGGGACCACT CGGGCACTCT GGGCGCAGAG GAGTTCAAGG CCTGCCTGAT CAGCCTGGGC 
TTCGATATCG CCAACGATGC ACAGGGTGAA AACGAGTTCT CCCGCATCAT GAGCATAGTG 
GATCCCAACA GGATGGGCAT CGTCACCTTC CAGGCCTTCA TCGACTTCAT GTCCCGCGAG 
ACGGCGGACA CGGACACCGC CGACCAGGTC ATGGCCTCCT TCAAGGTCCT GGCGGGCGAT 
AAGAACTACA TCTTAGCCGA CGAGCTTCGC CGTGAGCTTC CTCCAGACCA GGCTGATTAC 
TGCATCGCCC GCATGGCGCC CTACTGCGGC CCGGACGCCG TTCCCGGAGC CCTCGACTAC 
ATGTCCTTCT CCACGGCTCT GTACGGGGAG AGCGACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022052787.1
CDS	267..2948
Translation	MYNMDQYDGENDYMQQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPMTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRLPENTMQAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQnullKLRLSNRPAFMPSEGKMVSDINNAWGSLEGAEKGYEEWLLNEIRRLERLDHLAEKFRQKAAIHEAWTEGKEEMLQKRDYETASLSDIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQRICDQWDALGALTQKRSEALQRTEKLLETIDQLYLEFAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLSTAHEQFKATLPEADKERLAILGIHNEIAKIVQTYHVNMAGTNPYTTINPQEINAKWDKVRQLVPQRDQALIEEHARQQNNERLRRQFANQANVIGPWIQNKMEEIGRISIEMHGTLEDQLTHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEIENQILTRDAKGISQEQLNEFRASFNHFDRDHSGTLGAEEFKACLISLGFDIANDAQGENEFSRIMSIVDPNRMGIVTFQAFIDFMSRETADTDTADQVMASFKVLAGDKNYILADELRRELPPDQADYCIARMAPYCGPDAVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_022197095.1
Organism	Acanthochromis polyacanthus(spiny chromis)
Definition	Acanthochromis polyacanthus alpha-actinin-1 (LOC110953218), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022197095.1

ORF Insert Sequence:

ATGTATAATA TGGATCAGTA TGATGGAGAG AATGACTACA TGCAGCAGGA GGACGACTGG 
GACCGGGATC TGCTCCTGGA TCCTGCATGG GAGAAGCAGC AGAGAAAGAC ATTTACAGCC 
TGGTGTAACT CTCACCTGAG GAAAGCAGGA ACGCAGATCG AGAACATTGA GGAGGACTTC 
CGGGACGGCC TGAAACTCAT GCTGCTGCTG GAGGTCATCT CAGGTGAACG CTTGGCCAAA 
CCAGAAAGAG GCAAAATGAG AGTCCACAAG ATCTCCAACG TTAACAAAGC GCTCGACTTC 
ATCGCCAGCA AAGGAGTAAA GCTGGTTTCT ATTGGAGCAG AAGAGATCGT AGACGGCAAC 
GCTAAGATGA CCCTGGGAAT GATCTGGACT ATAATCCTCC GCTTCGCCAT CCAAGACATT 
TCTGTGGAAG AGACATCTGC CAAAGAGGGC CTCCTGCTGT GGTGCCAGAG GAAGACTGCG 
CCCTACAAGA ATGTCAACAT CCAGAACTTT CACATCAGCT GGAAAGACGG TTTGGGTTTC 
TGTGCACTCA TTCATCGACA CCGTCCAGAA CTCATCGACT ACGGAAAACT GCGCAAGGAC 
GACCCGATGA CCAACCTGAA CACAGCCTTC GACGTGGCGG AGAAGTACCT GGACATCCCC 
AAGATGTTGG ACGCAGAAGA CATTGTGGGC ACTGCCCGTC CGGACGAGAA GGCCATCATG 
ACCTACGTCT CTAGCTTCTA CCACGCCTTC TCAGGCGCCC AGAAGGCAGA GACGGCAGCC 
AACAGGATCT GCAAGGTGCT GGCTGTCAAC CAAGAGAATG AGCAGCTGAT GGAGGACTAT 
GAGAAGCTGG CCAGCGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC 
CGATTGCCCG AGAACACCAT GCAGGCCATG CAGCAGAAGC TGGAGGACTT CAGAGATTAC 
CGCCGCCTCC ACAAGCCGCC CAAAGTGCAG GAGAAGTGCC AGCTGGAGAT CAACTTCAAC 
ACCCTGCAGA CNAAGCTGAG GCTCAGCAAC AGGCCCGCCT TCATGCCCTC GGAGGGAAAG 
ATGGTCTCGG ACATTAACAA TGCATGGGGG AGCCTAGAGG GAGCAGAGAA GGGCTATGAA 
GAGTGGCTCC TCAATGAGAT TCGCCGGCTG GAAAGACTCG ATCACCTGGC AGAGAAATTC 
CGCCAGAAAG CAGCGATCCA TGAAGCCTGG ACTGAAGGTA AAGAAGAAAT GCTGCAGAAA 
CGGGACTACG AGACAGCCTC GCTGTCAGAT ATCAAGGCCT TGCTGAAGAA ACACGAGGCC 
TTCGAGAGCG ATCTGGCTGC ACACCAGGAC CGTGTGGAGC AGATTGCAGC CATCGCACAG 
GAGTTAAATG AGCTGGACTA CTACGACTCC CCCAGTGTAA ACGCTCGCTG CCAGCGGATC 
TGCGACCAAT GGGACGCGCT GGGAGCTCTG ACCCAGAAAC GCAGCGAGGC TTTGCAGAGA 
ACCGAGAAGC TGCTTGAGAC CATCGACCAA CTGTACCTTG AGTTTGCCAA AAGAGCGGCT 
CCGTTCAACA ACTGGATGGA GGGAGCCATG GAGGACCTGC AGGACACTTT TATCGTCCAC 
ACCATCGAGG AAATCCAGGG ATTGAGCACG GCCCACGAGC AGTTCAAAGC TACACTGCCA 
GAGGCCGACA AAGAGCGTCT GGCCATCCTG GGCATCCACA ACGAGATCGC CAAAATCGTG 
CAGACCTACC ACGTGAACAT GGCCGGAACC AACCCGTACA CCACCATCAA CCCACAGGAG 
ATCAACGCCA AATGGGACAA GGTCCGACAG CTGGTTCCTC AGCGCGACCA GGCTCTGATT 
GAGGAACACG CTCGGCAGCA GAACAACGAA CGTCTGCGCC GACAGTTCGC CAACCAGGCC 
AACGTCATCG GGCCCTGGAT CCAGAACAAG ATGGAGGAAA TCGGTCGTAT CTCGATTGAG 
ATGCACGGCA CCCTGGAGGA TCAGCTGACG CACCTCCGCC AGTACGAGAA AAGCATCGTC 
AACTACAAGC CAAAGATCGA CCAGCTGGAG GGAGACCACC AGCTCATTCA GGAAGCCCTC 
ATCTTCGACA ACAAGCACAC CAACTACACC ATGGAGCACA TCCGTGTGGG CTGGGAGCAG 
CTGCTCACCA CCATCGCCCG AACCATCAAC GAGATCGAGA ACCAGATCTT GACGAGAGAC 
GCCAAAGGCA TCAGCCAGGA GCAGCTGAAC GAGTTCCGGG CCTCCTTCAA CCACTTCGAC 
AGGGACCACT CGGGCACTCT GGGCGCAGAG GAGTTCAAGG CCTGCCTGAT CAGCCTGGGC 
TTCGATATCG CCAACGATGC ACAGGGTGAA AACGAGTTCT CCCGCATCAT GAGCATAGTG 
GATCCCAACA GGATGGGCAT CGTCACCTTC CAGGCCTTCA TCGACTTCAT GTCCCGCGAG 
ACGGCGGACA CGGACACCGC CGACCAGGTC ATGGCCTCCT TCAAGGTCCT GGCGGGCGAT 
AAGAACTACA TCTTAGCCGA CGAGCTTCGC CGTGAGCTTC CTCCAGACCA GGCTGATTAC 
TGCATCGCCC GCATGGCGCC CTACTGCGGC CCGGACGCCG TTCCCGGAGC CCTCGACTAC 
ATGTCCTTCT CCACGGCTCT GTACGGGGAG AGCGACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110953218 ( XM_022197096.1 ) cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis) -> XP_022052788.1 Acanthochromis polyacanthus alpha-actinin-1 (LOC110953218), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OAc194436
Clone ID Related Accession (Same CDS sequence)	XM_022197096.1
Accession Version	XM_022197096.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-06
Organism	Acanthochromis polyacanthus(spiny chromis)
Product	alpha-actinin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019029399.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthochromis polyacanthus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATAATA TGGATCAGTA TGATGGAGAG AATGACTACA TGCAGCAGGA GGACGACTGG 
GACCGGGATC TGCTCCTGGA TCCTGCATGG GAGAAGCAGC AGAGAAAGAC ATTTACAGCC 
TGGTGTAACT CTCACCTGAG GAAAGCAGGA ACGCAGATCG AGAACATTGA GGAGGACTTC 
CGGGACGGCC TGAAACTCAT GCTGCTGCTG GAGGTCATCT CAGGTGAACG CTTGGCCAAA 
CCAGAAAGAG GCAAAATGAG AGTCCACAAG ATCTCCAACG TTAACAAAGC GCTCGACTTC 
ATCGCCAGCA AAGGAGTAAA GCTGGTTTCT ATTGGAGCAG AAGAGATCGT AGACGGCAAC 
GCTAAGATGA CCCTGGGAAT GATCTGGACT ATAATCCTCC GCTTCGCCAT CCAAGACATT 
TCTGTGGAAG AGACATCTGC CAAAGAGGGC CTCCTGCTGT GGTGCCAGAG GAAGACTGCG 
CCCTACAAGA ATGTCAACAT CCAGAACTTT CACATCAGCT GGAAAGACGG TTTGGGTTTC 
TGTGCACTCA TTCATCGACA CCGTCCAGAA CTCATCGACT ACGGAAAACT GCGCAAGGAC 
GACCCGATGA CCAACCTGAA CACAGCCTTC GACGTGGCGG AGAAGTACCT GGACATCCCC 
AAGATGTTGG ACGCAGAAGA CATTGTGGGC ACTGCCCGTC CGGACGAGAA GGCCATCATG 
ACCTACGTCT CTAGCTTCTA CCACGCCTTC TCAGGCGCCC AGAAGGCAGA GACGGCAGCC 
AACAGGATCT GCAAGGTGCT GGCTGTCAAC CAAGAGAATG AGCAGCTGAT GGAGGACTAT 
GAGAAGCTGG CCAGCGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC 
CGATTGCCCG AGAACACCAT GCAGGCCATG CAGCAGAAGC TGGAGGACTT CAGAGATTAC 
CGCCGCCTCC ACAAGCCGCC CAAAGTGCAG GAGAAGTGCC AGCTGGAGAT CAACTTCAAC 
ACCCTGCAGA CNAAGCTGAG GCTCAGCAAC AGGCCCGCCT TCATGCCCTC GGAGGGAAAG 
ATGGTCTCGG ACATTAACAA TGCATGGGGG AGCCTAGAGG GAGCAGAGAA GGGCTATGAA 
GAGTGGCTCC TCAATGAGAT TCGCCGGCTG GAAAGACTCG ATCACCTGGC AGAGAAATTC 
CGCCAGAAAG CAGCGATCCA TGAAGCCTGG ACTGAAGGTA AAGAAGAAAT GCTGCAGAAA 
CGGGACTACG AGACAGCCTC GCTGTCAGAT ATCAAGGCCT TGCTGAAGAA ACACGAGGCC 
TTCGAGAGCG ATCTGGCTGC ACACCAGGAC CGTGTGGAGC AGATTGCAGC CATCGCACAG 
GAGTTAAATG AGCTGGACTA CTACGACTCC CCCAGTGTAA ACGCTCGCTG CCAGCGGATC 
TGCGACCAAT GGGACGCGCT GGGAGCTCTG ACCCAGAAAC GCAGCGAGGC TTTGCAGAGA 
ACCGAGAAGC TGCTTGAGAC CATCGACCAA CTGTACCTTG AGTTTGCCAA AAGAGCGGCT 
CCGTTCAACA ACTGGATGGA GGGAGCCATG GAGGACCTGC AGGACACTTT TATCGTCCAC 
ACCATCGAGG AAATCCAGGG ATTGAGCACG GCCCACGAGC AGTTCAAAGC TACACTGCCA 
GAGGCCGACA AAGAGCGTCT GGCCATCCTG GGCATCCACA ACGAGATCGC CAAAATCGTG 
CAGACCTACC ACGTGAACAT GGCCGGAACC AACCCGTACA CCACCATCAA CCCACAGGAG 
ATCAACGCCA AATGGGACAA GGTCCGACAG CTGGTTCCTC AGCGCGACCA GGCTCTGATT 
GAGGAACACG CTCGGCAGCA GAACAACGAA CGTCTGCGCC GACAGTTCGC CAACCAGGCC 
AACGTCATCG GGCCCTGGAT CCAGAACAAG ATGGAGGAAA TCGGTCGTAT CTCGATTGAG 
ATGCACGGCA CCCTGGAGGA TCAGCTGACG CACCTCCGCC AGTACGAGAA AAGCATCGTC 
AACTACAAGC CAAAGATCGA CCAGCTGGAG GGAGACCACC AGCTCATTCA GGAAGCCCTC 
ATCTTCGACA ACAAGCACAC CAACTACACC ATGGAGCACA TCCGTGTGGG CTGGGAGCAG 
CTGCTCACCA CCATCGCCCG AACCATCAAC GAGATCGAGA ACCAGATCTT GACGAGAGAC 
GCCAAAGGCA TCAGCCAGGA GCAGCTGAAC GAGTTCCGGG CCTCCTTCAA CCACTTCGAC 
AGGAAGAGAA CAGGAATCAT GGATGCTGAA GACTTCAAAA CCTGCCTTAT CTCCGTGGGT 
TACAACCTGG GTGAAAACGA GTTCTCCCGC ATCATGAGCA TAGTGGATCC CAACAGGATG 
GGCATCGTCA CCTTCCAGGC CTTCATCGAC TTCATGTCCC GCGAGACGGC GGACACGGAC 
ACCGCCGACC AGGTCATGGC CTCCTTCAAG GTCCTGGCGG GCGATAAGAA CTACATCTTA 
GCCGACGAGC TTCGCCGTGA GCTTCCTCCA GACCAGGCTG ATTACTGCAT CGCCCGCATG 
GCGCCCTACT GCGGCCCGGA CGCCGTTCCC GGAGCCCTCG ACTACATGTC CTTCTCCACG 
GCTCTGTACG GGGAGAGCGA CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022052788.1
CDS	267..2933
Translation	MYNMDQYDGENDYMQQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPMTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRLPENTMQAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQnullKLRLSNRPAFMPSEGKMVSDINNAWGSLEGAEKGYEEWLLNEIRRLERLDHLAEKFRQKAAIHEAWTEGKEEMLQKRDYETASLSDIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQRICDQWDALGALTQKRSEALQRTEKLLETIDQLYLEFAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLSTAHEQFKATLPEADKERLAILGIHNEIAKIVQTYHVNMAGTNPYTTINPQEINAKWDKVRQLVPQRDQALIEEHARQQNNERLRRQFANQANVIGPWIQNKMEEIGRISIEMHGTLEDQLTHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEIENQILTRDAKGISQEQLNEFRASFNHFDRKRTGIMDAEDFKTCLISVGYNLGENEFSRIMSIVDPNRMGIVTFQAFIDFMSRETADTDTADQVMASFKVLAGDKNYILADELRRELPPDQADYCIARMAPYCGPDAVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_022197096.1
Organism	Acanthochromis polyacanthus(spiny chromis)
Definition	Acanthochromis polyacanthus alpha-actinin-1 (LOC110953218), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022197096.1

ORF Insert Sequence:

ATGTATAATA TGGATCAGTA TGATGGAGAG AATGACTACA TGCAGCAGGA GGACGACTGG 
GACCGGGATC TGCTCCTGGA TCCTGCATGG GAGAAGCAGC AGAGAAAGAC ATTTACAGCC 
TGGTGTAACT CTCACCTGAG GAAAGCAGGA ACGCAGATCG AGAACATTGA GGAGGACTTC 
CGGGACGGCC TGAAACTCAT GCTGCTGCTG GAGGTCATCT CAGGTGAACG CTTGGCCAAA 
CCAGAAAGAG GCAAAATGAG AGTCCACAAG ATCTCCAACG TTAACAAAGC GCTCGACTTC 
ATCGCCAGCA AAGGAGTAAA GCTGGTTTCT ATTGGAGCAG AAGAGATCGT AGACGGCAAC 
GCTAAGATGA CCCTGGGAAT GATCTGGACT ATAATCCTCC GCTTCGCCAT CCAAGACATT 
TCTGTGGAAG AGACATCTGC CAAAGAGGGC CTCCTGCTGT GGTGCCAGAG GAAGACTGCG 
CCCTACAAGA ATGTCAACAT CCAGAACTTT CACATCAGCT GGAAAGACGG TTTGGGTTTC 
TGTGCACTCA TTCATCGACA CCGTCCAGAA CTCATCGACT ACGGAAAACT GCGCAAGGAC 
GACCCGATGA CCAACCTGAA CACAGCCTTC GACGTGGCGG AGAAGTACCT GGACATCCCC 
AAGATGTTGG ACGCAGAAGA CATTGTGGGC ACTGCCCGTC CGGACGAGAA GGCCATCATG 
ACCTACGTCT CTAGCTTCTA CCACGCCTTC TCAGGCGCCC AGAAGGCAGA GACGGCAGCC 
AACAGGATCT GCAAGGTGCT GGCTGTCAAC CAAGAGAATG AGCAGCTGAT GGAGGACTAT 
GAGAAGCTGG CCAGCGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC 
CGATTGCCCG AGAACACCAT GCAGGCCATG CAGCAGAAGC TGGAGGACTT CAGAGATTAC 
CGCCGCCTCC ACAAGCCGCC CAAAGTGCAG GAGAAGTGCC AGCTGGAGAT CAACTTCAAC 
ACCCTGCAGA CNAAGCTGAG GCTCAGCAAC AGGCCCGCCT TCATGCCCTC GGAGGGAAAG 
ATGGTCTCGG ACATTAACAA TGCATGGGGG AGCCTAGAGG GAGCAGAGAA GGGCTATGAA 
GAGTGGCTCC TCAATGAGAT TCGCCGGCTG GAAAGACTCG ATCACCTGGC AGAGAAATTC 
CGCCAGAAAG CAGCGATCCA TGAAGCCTGG ACTGAAGGTA AAGAAGAAAT GCTGCAGAAA 
CGGGACTACG AGACAGCCTC GCTGTCAGAT ATCAAGGCCT TGCTGAAGAA ACACGAGGCC 
TTCGAGAGCG ATCTGGCTGC ACACCAGGAC CGTGTGGAGC AGATTGCAGC CATCGCACAG 
GAGTTAAATG AGCTGGACTA CTACGACTCC CCCAGTGTAA ACGCTCGCTG CCAGCGGATC 
TGCGACCAAT GGGACGCGCT GGGAGCTCTG ACCCAGAAAC GCAGCGAGGC TTTGCAGAGA 
ACCGAGAAGC TGCTTGAGAC CATCGACCAA CTGTACCTTG AGTTTGCCAA AAGAGCGGCT 
CCGTTCAACA ACTGGATGGA GGGAGCCATG GAGGACCTGC AGGACACTTT TATCGTCCAC 
ACCATCGAGG AAATCCAGGG ATTGAGCACG GCCCACGAGC AGTTCAAAGC TACACTGCCA 
GAGGCCGACA AAGAGCGTCT GGCCATCCTG GGCATCCACA ACGAGATCGC CAAAATCGTG 
CAGACCTACC ACGTGAACAT GGCCGGAACC AACCCGTACA CCACCATCAA CCCACAGGAG 
ATCAACGCCA AATGGGACAA GGTCCGACAG CTGGTTCCTC AGCGCGACCA GGCTCTGATT 
GAGGAACACG CTCGGCAGCA GAACAACGAA CGTCTGCGCC GACAGTTCGC CAACCAGGCC 
AACGTCATCG GGCCCTGGAT CCAGAACAAG ATGGAGGAAA TCGGTCGTAT CTCGATTGAG 
ATGCACGGCA CCCTGGAGGA TCAGCTGACG CACCTCCGCC AGTACGAGAA AAGCATCGTC 
AACTACAAGC CAAAGATCGA CCAGCTGGAG GGAGACCACC AGCTCATTCA GGAAGCCCTC 
ATCTTCGACA ACAAGCACAC CAACTACACC ATGGAGCACA TCCGTGTGGG CTGGGAGCAG 
CTGCTCACCA CCATCGCCCG AACCATCAAC GAGATCGAGA ACCAGATCTT GACGAGAGAC 
GCCAAAGGCA TCAGCCAGGA GCAGCTGAAC GAGTTCCGGG CCTCCTTCAA CCACTTCGAC 
AGGAAGAGAA CAGGAATCAT GGATGCTGAA GACTTCAAAA CCTGCCTTAT CTCCGTGGGT 
TACAACCTGG GTGAAAACGA GTTCTCCCGC ATCATGAGCA TAGTGGATCC CAACAGGATG 
GGCATCGTCA CCTTCCAGGC CTTCATCGAC TTCATGTCCC GCGAGACGGC GGACACGGAC 
ACCGCCGACC AGGTCATGGC CTCCTTCAAG GTCCTGGCGG GCGATAAGAA CTACATCTTA 
GCCGACGAGC TTCGCCGTGA GCTTCCTCCA GACCAGGCTG ATTACTGCAT CGCCCGCATG 
GCGCCCTACT GCGGCCCGGA CGCCGTTCCC GGAGCCCTCG ACTACATGTC CTTCTCCACG 
GCTCTGTACG GGGAGAGCGA CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110953218 ( XM_022197097.1 ) cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis) -> XP_022052789.1 Acanthochromis polyacanthus alpha-actinin-1 (LOC110953218), transcript variant X5, mRNA.

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CloneID	OAc194437
Clone ID Related Accession (Same CDS sequence)	XM_022197097.1
Accession Version	XM_022197097.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-06
Organism	Acanthochromis polyacanthus(spiny chromis)
Product	alpha-actinin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019029399.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthochromis polyacanthus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATAATA TGGATCAGTA TGATGGAGAG AATGACTACA TGCAGCAGGA GGACGACTGG 
GACCGGGATC TGCTCCTGGA TCCTGCATGG GAGAAGCAGC AGAGAAAGAC ATTTACAGCC 
TGGTGTAACT CTCACCTGAG GAAAGCAGGA ACGCAGATCG AGAACATTGA GGAGGACTTC 
CGGGACGGCC TGAAACTCAT GCTGCTGCTG GAGGTCATCT CAGGTGAACG CTTGGCCAAA 
CCAGAAAGAG GCAAAATGAG AGTCCACAAG ATCTCCAACG TTAACAAAGC GCTCGACTTC 
ATCGCCAGCA AAGGAGTAAA GCTGGTTTCT ATTGGAGCAG AAGAGATCGT AGACGGCAAC 
GCTAAGATGA CCCTGGGAAT GATCTGGACT ATAATCCTCC GCTTCGCCAT CCAAGACATT 
TCTGTGGAAG AGACATCTGC CAAAGAGGGC CTCCTGCTGT GGTGCCAGAG GAAGACTGCG 
CCCTACAAGA ATGTCAACAT CCAGAACTTT CACATCAGCT GGAAAGACGG TTTGGGTTTC 
TGTGCACTCA TTCATCGACA CCGTCCAGAA CTCATCGACT ACGGAAAACT GCGCAAGGAC 
GACCCGATGA CCAACCTGAA CACAGCCTTC GACGTGGCGG AGAAGTACCT GGACATCCCC 
AAGATGTTGG ACGCAGAAGA TATCATTAGC ACCTTGAGGC CCGATGAGAA GGCCGTCATG 
ACTTATGTGT CGTGTTACTA CCACGCGTTC TCAGGCAAGC AGAAGGCAGA GACGGCAGCC 
AACAGGATCT GCAAGGTGCT GGCTGTCAAC CAAGAGAATG AGCAGCTGAT GGAGGACTAT 
GAGAAGCTGG CCAGCGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC 
CGATTGCCCG AGAACACCAT GCAGGCCATG CAGCAGAAGC TGGAGGACTT CAGAGATTAC 
CGCCGCCTCC ACAAGCCGCC CAAAGTGCAG GAGAAGTGCC AGCTGGAGAT CAACTTCAAC 
ACCCTGCAGA CNAAGCTGAG GCTCAGCAAC AGGCCCGCCT TCATGCCCTC GGAGGGAAAG 
ATGGTCTCGG ACATTAACAA TGCATGGGGG AGCCTAGAGG GAGCAGAGAA GGGCTATGAA 
GAGTGGCTCC TCAATGAGAT TCGCCGGCTG GAAAGACTCG ATCACCTGGC AGAGAAATTC 
CGCCAGAAAG CAGCGATCCA TGAAGCCTGG ACTGAAGGTA AAGAAGAAAT GCTGCAGAAA 
CGGGACTACG AGACAGCCTC GCTGTCAGAT ATCAAGGCCT TGCTGAAGAA ACACGAGGCC 
TTCGAGAGCG ATCTGGCTGC ACACCAGGAC CGTGTGGAGC AGATTGCAGC CATCGCACAG 
GAGTTAAATG AGCTGGACTA CTACGACTCC CCCAGTGTAA ACGCTCGCTG CCAGCGGATC 
TGCGACCAAT GGGACGCGCT GGGAGCTCTG ACCCAGAAAC GCAGCGAGGC TTTGCAGAGA 
ACCGAGAAGC TGCTTGAGAC CATCGACCAA CTGTACCTTG AGTTTGCCAA AAGAGCGGCT 
CCGTTCAACA ACTGGATGGA GGGAGCCATG GAGGACCTGC AGGACACTTT TATCGTCCAC 
ACCATCGAGG AAATCCAGGG ATTGAGCACG GCCCACGAGC AGTTCAAAGC TACACTGCCA 
GAGGCCGACA AAGAGCGTCT GGCCATCCTG GGCATCCACA ACGAGATCGC CAAAATCGTG 
CAGACCTACC ACGTGAACAT GGCCGGAACC AACCCGTACA CCACCATCAA CCCACAGGAG 
ATCAACGCCA AATGGGACAA GGTCCGACAG CTGGTTCCTC AGCGCGACCA GGCTCTGATT 
GAGGAACACG CTCGGCAGCA GAACAACGAA CGTCTGCGCC GACAGTTCGC CAACCAGGCC 
AACGTCATCG GGCCCTGGAT CCAGAACAAG ATGGAGGAAA TCGGTCGTAT CTCGATTGAG 
ATGCACGGCA CCCTGGAGGA TCAGCTGACG CACCTCCGCC AGTACGAGAA AAGCATCGTC 
AACTACAAGC CAAAGATCGA CCAGCTGGAG GGAGACCACC AGCTCATTCA GGAAGCCCTC 
ATCTTCGACA ACAAGCACAC CAACTACACC ATGGAGCACA TCCGTGTGGG CTGGGAGCAG 
CTGCTCACCA CCATCGCCCG AACCATCAAC GAGATCGAGA ACCAGATCTT GACGAGAGAC 
GCCAAAGGCA TCAGCCAGGA GCAGCTGAAC GAGTTCCGGG CCTCCTTCAA CCACTTCGAC 
AGGAAGAGAA CAGGAATCAT GGATGCTGAA GACTTCAAAA CCTGCCTTAT CTCCGTGGGT 
TACAACCTGG GTGAAAACGA GTTCTCCCGC ATCATGAGCA TAGTGGATCC CAACAGGATG 
GGCATCGTCA CCTTCCAGGC CTTCATCGAC TTCATGTCCC GCGAGACGGC GGACACGGAC 
ACCGCCGACC AGGTCATGGC CTCCTTCAAG GTCCTGGCGG GCGATAAGAA CTACATCTTA 
GCCGACGAGC TTCGCCGTGA GCTTCCTCCA GACCAGGCTG ATTACTGCAT CGCCCGCATG 
GCGCCCTACT GCGGCCCGGA CGCCGTTCCC GGAGCCCTCG ACTACATGTC CTTCTCCACG 
GCTCTGTACG GGGAGAGCGA CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022052789.1
CDS	267..2933
Translation	MYNMDQYDGENDYMQQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPMTNLNTAFDVAEKYLDIPKMLDAEDIISTLRPDEKAVMTYVSCYYHAFSGKQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRLPENTMQAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQnullKLRLSNRPAFMPSEGKMVSDINNAWGSLEGAEKGYEEWLLNEIRRLERLDHLAEKFRQKAAIHEAWTEGKEEMLQKRDYETASLSDIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQRICDQWDALGALTQKRSEALQRTEKLLETIDQLYLEFAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLSTAHEQFKATLPEADKERLAILGIHNEIAKIVQTYHVNMAGTNPYTTINPQEINAKWDKVRQLVPQRDQALIEEHARQQNNERLRRQFANQANVIGPWIQNKMEEIGRISIEMHGTLEDQLTHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEIENQILTRDAKGISQEQLNEFRASFNHFDRKRTGIMDAEDFKTCLISVGYNLGENEFSRIMSIVDPNRMGIVTFQAFIDFMSRETADTDTADQVMASFKVLAGDKNYILADELRRELPPDQADYCIARMAPYCGPDAVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_022197097.1
Organism	Acanthochromis polyacanthus(spiny chromis)
Definition	Acanthochromis polyacanthus alpha-actinin-1 (LOC110953218), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022197097.1

ORF Insert Sequence:

ATGTATAATA TGGATCAGTA TGATGGAGAG AATGACTACA TGCAGCAGGA GGACGACTGG 
GACCGGGATC TGCTCCTGGA TCCTGCATGG GAGAAGCAGC AGAGAAAGAC ATTTACAGCC 
TGGTGTAACT CTCACCTGAG GAAAGCAGGA ACGCAGATCG AGAACATTGA GGAGGACTTC 
CGGGACGGCC TGAAACTCAT GCTGCTGCTG GAGGTCATCT CAGGTGAACG CTTGGCCAAA 
CCAGAAAGAG GCAAAATGAG AGTCCACAAG ATCTCCAACG TTAACAAAGC GCTCGACTTC 
ATCGCCAGCA AAGGAGTAAA GCTGGTTTCT ATTGGAGCAG AAGAGATCGT AGACGGCAAC 
GCTAAGATGA CCCTGGGAAT GATCTGGACT ATAATCCTCC GCTTCGCCAT CCAAGACATT 
TCTGTGGAAG AGACATCTGC CAAAGAGGGC CTCCTGCTGT GGTGCCAGAG GAAGACTGCG 
CCCTACAAGA ATGTCAACAT CCAGAACTTT CACATCAGCT GGAAAGACGG TTTGGGTTTC 
TGTGCACTCA TTCATCGACA CCGTCCAGAA CTCATCGACT ACGGAAAACT GCGCAAGGAC 
GACCCGATGA CCAACCTGAA CACAGCCTTC GACGTGGCGG AGAAGTACCT GGACATCCCC 
AAGATGTTGG ACGCAGAAGA TATCATTAGC ACCTTGAGGC CCGATGAGAA GGCCGTCATG 
ACTTATGTGT CGTGTTACTA CCACGCGTTC TCAGGCAAGC AGAAGGCAGA GACGGCAGCC 
AACAGGATCT GCAAGGTGCT GGCTGTCAAC CAAGAGAATG AGCAGCTGAT GGAGGACTAT 
GAGAAGCTGG CCAGCGATCT GCTGGAGTGG ATCCGTCGCA CCATCCCCTG GCTGGAGAAC 
CGATTGCCCG AGAACACCAT GCAGGCCATG CAGCAGAAGC TGGAGGACTT CAGAGATTAC 
CGCCGCCTCC ACAAGCCGCC CAAAGTGCAG GAGAAGTGCC AGCTGGAGAT CAACTTCAAC 
ACCCTGCAGA CNAAGCTGAG GCTCAGCAAC AGGCCCGCCT TCATGCCCTC GGAGGGAAAG 
ATGGTCTCGG ACATTAACAA TGCATGGGGG AGCCTAGAGG GAGCAGAGAA GGGCTATGAA 
GAGTGGCTCC TCAATGAGAT TCGCCGGCTG GAAAGACTCG ATCACCTGGC AGAGAAATTC 
CGCCAGAAAG CAGCGATCCA TGAAGCCTGG ACTGAAGGTA AAGAAGAAAT GCTGCAGAAA 
CGGGACTACG AGACAGCCTC GCTGTCAGAT ATCAAGGCCT TGCTGAAGAA ACACGAGGCC 
TTCGAGAGCG ATCTGGCTGC ACACCAGGAC CGTGTGGAGC AGATTGCAGC CATCGCACAG 
GAGTTAAATG AGCTGGACTA CTACGACTCC CCCAGTGTAA ACGCTCGCTG CCAGCGGATC 
TGCGACCAAT GGGACGCGCT GGGAGCTCTG ACCCAGAAAC GCAGCGAGGC TTTGCAGAGA 
ACCGAGAAGC TGCTTGAGAC CATCGACCAA CTGTACCTTG AGTTTGCCAA AAGAGCGGCT 
CCGTTCAACA ACTGGATGGA GGGAGCCATG GAGGACCTGC AGGACACTTT TATCGTCCAC 
ACCATCGAGG AAATCCAGGG ATTGAGCACG GCCCACGAGC AGTTCAAAGC TACACTGCCA 
GAGGCCGACA AAGAGCGTCT GGCCATCCTG GGCATCCACA ACGAGATCGC CAAAATCGTG 
CAGACCTACC ACGTGAACAT GGCCGGAACC AACCCGTACA CCACCATCAA CCCACAGGAG 
ATCAACGCCA AATGGGACAA GGTCCGACAG CTGGTTCCTC AGCGCGACCA GGCTCTGATT 
GAGGAACACG CTCGGCAGCA GAACAACGAA CGTCTGCGCC GACAGTTCGC CAACCAGGCC 
AACGTCATCG GGCCCTGGAT CCAGAACAAG ATGGAGGAAA TCGGTCGTAT CTCGATTGAG 
ATGCACGGCA CCCTGGAGGA TCAGCTGACG CACCTCCGCC AGTACGAGAA AAGCATCGTC 
AACTACAAGC CAAAGATCGA CCAGCTGGAG GGAGACCACC AGCTCATTCA GGAAGCCCTC 
ATCTTCGACA ACAAGCACAC CAACTACACC ATGGAGCACA TCCGTGTGGG CTGGGAGCAG 
CTGCTCACCA CCATCGCCCG AACCATCAAC GAGATCGAGA ACCAGATCTT GACGAGAGAC 
GCCAAAGGCA TCAGCCAGGA GCAGCTGAAC GAGTTCCGGG CCTCCTTCAA CCACTTCGAC 
AGGAAGAGAA CAGGAATCAT GGATGCTGAA GACTTCAAAA CCTGCCTTAT CTCCGTGGGT 
TACAACCTGG GTGAAAACGA GTTCTCCCGC ATCATGAGCA TAGTGGATCC CAACAGGATG 
GGCATCGTCA CCTTCCAGGC CTTCATCGAC TTCATGTCCC GCGAGACGGC GGACACGGAC 
ACCGCCGACC AGGTCATGGC CTCCTTCAAG GTCCTGGCGG GCGATAAGAA CTACATCTTA 
GCCGACGAGC TTCGCCGTGA GCTTCCTCCA GACCAGGCTG ATTACTGCAT CGCCCGCATG 
GCGCCCTACT GCGGCCCGGA CGCCGTTCCC GGAGCCCTCG ACTACATGTC CTTCTCCACG 
GCTCTGTACG GGGAGAGCGA CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC110953218 cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム