RASAL2 cDNA ORF clone, Ovis aries(sheep)

The following RASAL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASAL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OOv11437 XM_012126643.1
Latest version!
Ovis aries musimon RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OOv11438 XM_012126644.1
Latest version!
Ovis aries musimon RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OOv11439 XM_012126645.1
Latest version!
Ovis aries musimon RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OOv11440 XM_012126647.2
Latest version!
Ovis aries musimon RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OOv11441 XM_012126648.2
Latest version!
Ovis aries musimon RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OOv11442 XM_012126646.2
Latest version!
Ovis aries musimon RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OOv11443 XM_012126649.2
Latest version!
Ovis aries musimon RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OOv11444 XM_012126650.2
Latest version!
Ovis aries musimon RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OSm51966 XM_015099332.1
Latest version!
Ovis aries RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OSm61880 XM_027975902.1
Latest version!
Ovis aries RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OSm61881 XM_027975903.1
Latest version!
Ovis aries RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OSm61882 XM_027975904.1
Latest version!
Ovis aries RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OSm61883 XM_027975905.1
Latest version!
Ovis aries RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OSm61884 XM_027975906.1
Latest version!
Ovis aries RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OSm61885 XM_027975907.1
Latest version!
Ovis aries RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OSm61886 XM_015099332.2
Latest version!
Ovis aries RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OSm61887 XM_027975908.1
Latest version!
Ovis aries RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OOv11437
    Clone ID Related Accession (Same CDS sequence) XM_012126643.1
    Accession Version XM_012126643.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4272bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product ras GTPase-activating protein nGAP isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGTCAT GGAAGATGAG CATCTTTAAA AGACAAACAT CACCGCGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATGCA CACTCAGAGA TAGGAAGAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCTAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC CCAGCTTAAG GTTGAAAGGG
    GGCAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACA ACAACAAGCG
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGAACA
    TCAATGGCGA AGCAGAACCT GGTAAACACT CTGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCCG CTCCAGTGTC AGATTTGTTA GACGGTGGCC GACTTCAGTG
    GGGTCAGTGG AGTCTGAGCA GCAGGGATAT TTCATCCGTG GCGTTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGAGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGTC AGTCACCCTA CACCGACACG
    ACCACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA GGGAGGACAA GAGCAGCAGG CAGATTCCAC CAAAGGGCGA
    TGCCTGCGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCGCTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAATCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA ATCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCT
    GTGGAATGTC TGGATCTTGG TAGAGGGGAG CCTGTATCAG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TTCGTAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACCA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTA GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGTACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGAAT TAAATCACGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAACTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTAGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCTTTGTATG AGTCAGATGA AAACTGTGAA GTCGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGCGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA AGACATCAGC GAGCGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC CCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA AGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTGTGGGAAG TAGTTTCCCA ACTTGATAAG GGTGAAAATT CCTTCCTACA GGCGACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CTAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA AACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGTCGCA CGCCGCCCAA
    GCGGAGCATC CGTCTGTGAT GCTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CCGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CTTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG ACGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCAGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCGGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TTCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAAAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGAGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTCGT GCAGGAGCAG
    CAGATGCAGA GGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCCGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG ACTAATGGCC
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCGAAGCAA
    AAAATAATCG ATGCACAGGT CATAAATGGA GATGAGATTA TGCAAACAGG AAAAACGGAT
    CGTGTCGCTG GATTCGGCCA ACACCAGACT GATGAGCGCG CTGACCCAGG TGAAGGAACG
    GTACAGCATG CAGGTCCGAA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC
    GGAGAATGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011982033.1
    CDS3945..8216
    Translation

    Target ORF information:

    RefSeq Version XM_012126643.1
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012126643.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGTCAT GGAAGATGAG CATCTTTAAA AGACAAACAT CACCGCGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATGCA CACTCAGAGA TAGGAAGAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCTAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC CCAGCTTAAG GTTGAAAGGG
    GGCAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACA ACAACAAGCG
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGAACA
    TCAATGGCGA AGCAGAACCT GGTAAACACT CTGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCCG CTCCAGTGTC AGATTTGTTA GACGGTGGCC GACTTCAGTG
    GGGTCAGTGG AGTCTGAGCA GCAGGGATAT TTCATCCGTG GCGTTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGAGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGTC AGTCACCCTA CACCGACACG
    ACCACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA GGGAGGACAA GAGCAGCAGG CAGATTCCAC CAAAGGGCGA
    TGCCTGCGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCGCTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAATCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA ATCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCT
    GTGGAATGTC TGGATCTTGG TAGAGGGGAG CCTGTATCAG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TTCGTAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACCA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTA GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGTACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGAAT TAAATCACGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAACTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTAGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCTTTGTATG AGTCAGATGA AAACTGTGAA GTCGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGCGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA AGACATCAGC GAGCGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC CCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA AGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTGTGGGAAG TAGTTTCCCA ACTTGATAAG GGTGAAAATT CCTTCCTACA GGCGACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CTAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA AACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGTCGCA CGCCGCCCAA
    GCGGAGCATC CGTCTGTGAT GCTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CCGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CTTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG ACGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCAGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCGGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TTCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAAAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGAGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTCGT GCAGGAGCAG
    CAGATGCAGA GGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCCGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG ACTAATGGCC
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCGAAGCAA
    AAAATAATCG ATGCACAGGT CATAAATGGA GATGAGATTA TGCAAACAGG AAAAACGGAT
    CGTGTCGCTG GATTCGGCCA ACACCAGACT GATGAGCGCG CTGACCCAGG TGAAGGAACG
    GTACAGCATG CAGGTCCGAA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC
    GGAGAATGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv11438
    Clone ID Related Accession (Same CDS sequence) XM_012126644.1
    Accession Version XM_012126644.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4263bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product ras GTPase-activating protein nGAP isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGTCAT GGAAGATGAG CATCTTTAAA AGACAAACAT CACCGCGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATGCA CACTCAGAGA TAGGAAGAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCTAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC CCAGCTTAAG GTTGAAAGGG
    GGCAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACA ACAACAAGCG
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGAACA
    TCAATGGCGA AGCAGAACCT GGTAAACACT CTGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCCG CTCCAGTGTC AGATTTGTTA GACGGTGGCC GACTTCAGTG
    GGGTCAGTGG AGTCTGAGCA GCAGGGATAT TTCATCCGTG GCGTTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGAGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGTC AGTCACCCTA CACCGACACG
    ACCACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA GGGAGGACAA GAGCAGCAGG CAGATTCCAC CAAAGGGCGA
    TGCCTGCGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCGCTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAATCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA ATCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCT
    GTGGAATGTC TGGATCTTGG TAGAGGGGAG CCTGTATCAG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TTCGTAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACCA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTA GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGTACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGAAT TAAATCACGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAACTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTAGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCTTTGTATG AGTCAGATGA AAACTGTGAA GTCGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGCGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA AGACATCAGC GAGCGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC CCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA AGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTGTGGGAAG TAGTTTCCCA ACTTGATAAG GGTGAAAATT CCTTCCTACA GGCGACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CTAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA AACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGTCGCA CGCCGCCCAA
    GCGGAGCATC CGTCTGTGAT GCTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CCGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CTTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG ACGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCAGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCGGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TTCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAAAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGAGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTCGT GCAGGAGCAG
    CAGATGCAGA GGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCCGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG ACTAATGGCC
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCGAAGCAA
    AAAATAATCG ATGCACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA CACCAGACTG
    ATGAGCGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA TGGCATCTCC
    CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011982034.1
    CDS3945..8207
    Translation

    Target ORF information:

    RefSeq Version XM_012126644.1
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012126644.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGTCAT GGAAGATGAG CATCTTTAAA AGACAAACAT CACCGCGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATGCA CACTCAGAGA TAGGAAGAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCTAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC CCAGCTTAAG GTTGAAAGGG
    GGCAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACA ACAACAAGCG
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGAACA
    TCAATGGCGA AGCAGAACCT GGTAAACACT CTGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCCG CTCCAGTGTC AGATTTGTTA GACGGTGGCC GACTTCAGTG
    GGGTCAGTGG AGTCTGAGCA GCAGGGATAT TTCATCCGTG GCGTTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGAGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGTC AGTCACCCTA CACCGACACG
    ACCACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA GGGAGGACAA GAGCAGCAGG CAGATTCCAC CAAAGGGCGA
    TGCCTGCGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCGCTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAATCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA ATCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCT
    GTGGAATGTC TGGATCTTGG TAGAGGGGAG CCTGTATCAG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TTCGTAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACCA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTA GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGTACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGAAT TAAATCACGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAACTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTAGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCTTTGTATG AGTCAGATGA AAACTGTGAA GTCGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGCGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA AGACATCAGC GAGCGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC CCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA AGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTGTGGGAAG TAGTTTCCCA ACTTGATAAG GGTGAAAATT CCTTCCTACA GGCGACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CTAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA AACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGTCGCA CGCCGCCCAA
    GCGGAGCATC CGTCTGTGAT GCTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CCGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CTTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG ACGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCAGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCGGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TTCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAAAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGAGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTCGT GCAGGAGCAG
    CAGATGCAGA GGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCCGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG ACTAATGGCC
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCGAAGCAA
    AAAATAATCG ATGCACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA CACCAGACTG
    ATGAGCGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA TGGCATCTCC
    CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv11439
    Clone ID Related Accession (Same CDS sequence) XM_012126645.1
    Accession Version XM_012126645.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product ras GTPase-activating protein nGAP isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAGTCAT GGAAGATGAG CATCTTTAAA AGACAAACAT CACCGCGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATGCA CACTCAGAGA TAGGAAGAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCTAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC CCAGCTTAAG GTTGAAAGGG
    GGCAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACA ACAACAAGCG
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGAACA
    TCAATGGCGA AGCAGAACCT GGTAAACACT CTGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCCG CTCCAGTGTC AGATTTGTTA GACGGTGGCC GACTTCAGTG
    GGGTCAGTGG AGTCTGAGCA GCAGGGATAT TTCATCCGTG GCGTTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGAGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGTC AGTCACCCTA CACCGACACG
    ACCACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA GGGAGGACAA GAGCAGCAGG CAGATTCCAC CAAAGGGCGA
    TGCCTGCGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCGCTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAATCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA ATCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCT
    GTGGAATGTC TGGATCTTGG TAGAGGGGAG CCTGTATCAG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TTCGTAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACCA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTA GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGTACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGAAT TAAATCACGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAACTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTAGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCTTTGTATG AGTCAGATGA AAACTGTGAA GTCGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGCGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA AGACATCAGC GAGCGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC CCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA AGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTGTGGGAAG TAGTTTCCCA ACTTGATAAG GCGACCGTGG CAAAATTGGG ACCTCTCCCT
    CGTGTTCTTG CTGATATTAC TAAATCTCTG ACTAATCCTA CACCAATACA ACAGCAACTG
    AGACGCTTCA CTGAGCATAA CTCCAGTCCA AATGTCAGTG GAAGCCTCTC CTCTGGGCTG
    CAGAAAATAT TTGAAGACCC TACTGACAGT GATTTGCACA AACTAAAATC CCCAAGCCAG
    GACAACACAG ACAGTTATTT CAGAGGGAAA ACATTATTGC TGGTCCAGCA AGCCTCCTCC
    CAGAGCATGA CTTACTCGGA AAAGGATGAA AAGGAAAGTA GCCTCCCCAA TGGCCGCAGC
    ATCTCCCTCA TGGACCTTCA GGAGTCGCAC GCCGCCCAAG CGGAGCATCC GTCTGTGATG
    CTCGACGTGC CCATGCGCTT GACCGGAAGC CAGCTCTCCA TAACTCAGGT GGCCAGCATC
    AAGCAACTGC GGGAGACCCA GAGCACCCCC CAGAGTGCAC CCCAAGTGCG AAGGCCCCTG
    CACCCAGCCT TGAACCAGCC AGGCAGCCTC CAGCCCCTGT CCTTCCAGAA CCCTGTCTAT
    CACCTCAGTA ACCCCATTCC CGCAATGCCA AAGGCCTCTG TGGACTCCAG TTTGGAGAAC
    TTAAGCACTG CCAGTTCCAG GAGCCAAAGT AACAGTGAAG ACTTCAAACT CAGCGGACCC
    AGCAACAGCA GCATGGAGGA CTTCACCAAA CGGAGCACGC AGAGTGAGGA CTTCTCCAGA
    CGCCACACCA TGCCCGACAG ACACATACCT CTCGCCCTGC CGCGGCAGAA CAGCACGGGG
    CAGACCCAGC TCCGGAAGAT GGACCCAGCG GGGCTGGGCG CCCGAGCCAA AGCCCCGCCG
    TCCCTGCCGC ACAGCGCCTC CCTGCGGAGC ACCGGGAGCA TGTCGGTGGC ATCGGCGGCT
    CTGGTGGCCG AGCCCCTGCA GAACGGGAGC CGGTCCCGAC AGCAGTCCTC TTCCTCCAGA
    GAGAGCCCGG TCCCCAAAGT GAGAGCCATC CAGAGGCAGC AGACCCAGCA GGTTCAGTCA
    CCTGTGGACT CCGCCACAAT GTCCCCAGTC GAGAGGACAG CAGCCTGGGT TCTGAACAAT
    GGGCAGTATG AAGAGGACGT GGAAGAAACT GAGCAAAATC AGGACGAAGC CAAGCATGCT
    GAGAAGTACG AACAGGAAAT CACCAAACTG AAGGAGCGCC TGCGAGTGTC CAGCCGGCGA
    CTCGAGGAGT ATGAGCGCCG GTTGCTCGTG CAGGAGCAGC AGATGCAGAG GCTGCTGCTG
    GAGTACAAGG CCCGGCTGGA GGACAGCGAG GAGCGGCTGC GCCGGCAGCA GGAGGAGAAG
    GACAGCCAGA TGAAGAGCAT CATCAGCAGA CTAATGGCCG TGGAAGAGGA ACTGAAGAAG
    GATCATGCTG AGATGCAGGC AGTTATTGAT GCGAAGCAAA AAATAATCGA TGCACAGGTC
    ATAAATGGAG ATGAGATTAT GCAAACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA
    CACCAGACTG ATGAGCGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA
    TGGCATCTCC CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011982035.1
    CDS3945..8195
    Translation

    Target ORF information:

    RefSeq Version XM_012126645.1
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012126645.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAGTCAT GGAAGATGAG CATCTTTAAA AGACAAACAT CACCGCGTCC TCCAAGCTAT 
    CGAAGATCTT TGTCAGAACC TGCTTATGCA CACTCAGAGA TAGGAAGAAG CTTAAAGAGT
    TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCTAGT
    CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC CCAGCTTAAG GTTGAAAGGG
    GGCAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACA ACAACAAGCG
    GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAATGAAGT CCTTGGAACA
    TCAATGGCGA AGCAGAACCT GGTAAACACT CTGTCACTTC GGGTTCCATT CAGCAGATCT
    CTTTCAGAGC CAGAACCCCG CTCCAGTGTC AGATTTGTTA GACGGTGGCC GACTTCAGTG
    GGGTCAGTGG AGTCTGAGCA GCAGGGATAT TTCATCCGTG GCGTTTTCTC TACTCTTTCC
    AATGGCTTCT CCAGGATGCT GAGTCTAAAA GGAGAGTCAA CCAGTGAGCC AGTAAGAGAA
    GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGTC AGTCACCCTA CACCGACACG
    ACCACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG
    GAGAAGGAGA TACCTGTGGA GGGAGGACAA GAGCAGCAGG CAGATTCCAC CAAAGGGCGA
    TGCCTGCGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG
    GGCGCCGCTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG
    ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA
    CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAATCAAAG
    CTTGACCGAA ACACGAGCTT TCGGCTTCCC TCTCTTCGCA ATACCGATGA CAGGTCTCGT
    GGGCTGCCTA AACTAAAAGA ATCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCT
    GTGGAATGTC TGGATCTTGG TAGAGGGGAG CCTGTATCAG TAAAGCCACT CCATAGCAGC
    ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT
    TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TTCGTAGGAC AGTTCAACCA
    AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG
    GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT
    CGTACAACCA GCAAGACCAA AGCAGACAAT ATTTTCTGGG GTGAACATTT TGAGTTCTAC
    AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG
    AAAAAAGACA AGAATAATTA TGTAGGGCTA GTCAACATCC CCACTGCCAG TGTGACTGGT
    CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGTACACCTA CACCCAACAA AGGAAAGACA
    GGAGGACCTT CTATTCGAAT TAAATCACGT TTCCAAACCA TCACCATTCT GCCTATGGAG
    CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT
    GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAACTAGCCT GTGCCTTAGT GCACATTCTT
    CAAAGCACTG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT
    TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG
    GAATACCTCA AGTTAGTGGG ACAACAGTAT CTTCACGATG CACTGGGGGA GTTCATCAAA
    GCTTTGTATG AGTCAGATGA AAACTGTGAA GTCGATCCCA GCAAATGTTC ATCCAGTGAA
    CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC
    AACTCTTACT GTGTTTTTCC TCGCGAGCTG AAAGAAGTGT TTGCATCCTG GAAGCAGCAG
    TGCCTGAACC GCGGCAAGCA AGACATCAGC GAGCGGCTCA TCAGCGCCTC GCTGTTTCTC
    CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT
    GATGACCGCA CATCTCGGAC CCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC
    TTTGCCAAAT TTGGTAACAA AGAGGAATAC ATGGCATTCA TGAATGATTT TTTAGAACAT
    GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC CATCTCAAAC
    ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA
    CTGTGGGAAG TAGTTTCCCA ACTTGATAAG GCGACCGTGG CAAAATTGGG ACCTCTCCCT
    CGTGTTCTTG CTGATATTAC TAAATCTCTG ACTAATCCTA CACCAATACA ACAGCAACTG
    AGACGCTTCA CTGAGCATAA CTCCAGTCCA AATGTCAGTG GAAGCCTCTC CTCTGGGCTG
    CAGAAAATAT TTGAAGACCC TACTGACAGT GATTTGCACA AACTAAAATC CCCAAGCCAG
    GACAACACAG ACAGTTATTT CAGAGGGAAA ACATTATTGC TGGTCCAGCA AGCCTCCTCC
    CAGAGCATGA CTTACTCGGA AAAGGATGAA AAGGAAAGTA GCCTCCCCAA TGGCCGCAGC
    ATCTCCCTCA TGGACCTTCA GGAGTCGCAC GCCGCCCAAG CGGAGCATCC GTCTGTGATG
    CTCGACGTGC CCATGCGCTT GACCGGAAGC CAGCTCTCCA TAACTCAGGT GGCCAGCATC
    AAGCAACTGC GGGAGACCCA GAGCACCCCC CAGAGTGCAC CCCAAGTGCG AAGGCCCCTG
    CACCCAGCCT TGAACCAGCC AGGCAGCCTC CAGCCCCTGT CCTTCCAGAA CCCTGTCTAT
    CACCTCAGTA ACCCCATTCC CGCAATGCCA AAGGCCTCTG TGGACTCCAG TTTGGAGAAC
    TTAAGCACTG CCAGTTCCAG GAGCCAAAGT AACAGTGAAG ACTTCAAACT CAGCGGACCC
    AGCAACAGCA GCATGGAGGA CTTCACCAAA CGGAGCACGC AGAGTGAGGA CTTCTCCAGA
    CGCCACACCA TGCCCGACAG ACACATACCT CTCGCCCTGC CGCGGCAGAA CAGCACGGGG
    CAGACCCAGC TCCGGAAGAT GGACCCAGCG GGGCTGGGCG CCCGAGCCAA AGCCCCGCCG
    TCCCTGCCGC ACAGCGCCTC CCTGCGGAGC ACCGGGAGCA TGTCGGTGGC ATCGGCGGCT
    CTGGTGGCCG AGCCCCTGCA GAACGGGAGC CGGTCCCGAC AGCAGTCCTC TTCCTCCAGA
    GAGAGCCCGG TCCCCAAAGT GAGAGCCATC CAGAGGCAGC AGACCCAGCA GGTTCAGTCA
    CCTGTGGACT CCGCCACAAT GTCCCCAGTC GAGAGGACAG CAGCCTGGGT TCTGAACAAT
    GGGCAGTATG AAGAGGACGT GGAAGAAACT GAGCAAAATC AGGACGAAGC CAAGCATGCT
    GAGAAGTACG AACAGGAAAT CACCAAACTG AAGGAGCGCC TGCGAGTGTC CAGCCGGCGA
    CTCGAGGAGT ATGAGCGCCG GTTGCTCGTG CAGGAGCAGC AGATGCAGAG GCTGCTGCTG
    GAGTACAAGG CCCGGCTGGA GGACAGCGAG GAGCGGCTGC GCCGGCAGCA GGAGGAGAAG
    GACAGCCAGA TGAAGAGCAT CATCAGCAGA CTAATGGCCG TGGAAGAGGA ACTGAAGAAG
    GATCATGCTG AGATGCAGGC AGTTATTGAT GCGAAGCAAA AAATAATCGA TGCACAGGTC
    ATAAATGGAG ATGAGATTAT GCAAACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA
    CACCAGACTG ATGAGCGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA
    TGGCATCTCC CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv11440
    Clone ID Related Accession (Same CDS sequence) XM_012126647.2
    Accession Version XM_012126647.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3864bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product ras GTPase-activating protein nGAP isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012126647.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ACACCGACAC GACCACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA AGAGCAGCAG
    GCAGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCGCT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAATCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCTCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AATCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC TGTGGAATGT CTGGATCTTG GTAGAGGGGA GCCTGTATCA
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGAA TTAAATCACG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAACTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTAGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCTTTGTAT GAGTCAGATG AAAACTGTGA AGTCGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGCGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AAGACATCAG CGAGCGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CCCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AAGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT
    TCCTTCCTAC AGGCGACCGT GGCAAAATTG GGACCTCTCC CTCGTGTTCT TGCTGATATT
    ACTAAATCTC TGACTAATCC TACACCAATA CAACAGCAAC TGAGACGCTT CACTGAGCAT
    AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC
    CCTACTGACA GTGATTTGCA CAAACTAAAA TCCCCAAGCC AGGACAACAC AGACAGTTAT
    TTCAGAGGGA AAACATTATT GCTGGTCCAG CAAGCCTCCT CCCAGAGCAT GACTTACTCG
    GAAAAGGATG AAAAGGAAAG TAGCCTCCCC AATGGCCGCA GCATCTCCCT CATGGACCTT
    CAGGAGTCGC ACGCCGCCCA AGCGGAGCAT CCGTCTGTGA TGCTCGACGT GCCCATGCGC
    TTGACCGGAA GCCAGCTCTC CATAACTCAG GTGGCCAGCA TCAAGCAACT GCGGGAGACC
    CAGAGCACCC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCC TGCACCCAGC CTTGAACCAG
    CCAGGCAGCC TCCAGCCCCT GTCCTTCCAG AACCCTGTCT ATCACCTCAG TAACCCCATT
    CCCGCAATGC CAAAGGCCTC TGTGGACTCC AGTTTGGAGA ACTTAAGCAC TGCCAGTTCC
    AGGAGCCAAA GTAACAGTGA AGACTTCAAA CTCAGCGGAC CCAGCAACAG CAGCATGGAG
    GACTTCACCA AACGGAGCAC GCAGAGTGAG GACTTCTCCA GACGCCACAC CATGCCCGAC
    AGACACATAC CTCTCGCCCT GCCGCGGCAG AACAGCACGG GGCAGACCCA GCTCCGGAAG
    ATGGACCCAG CGGGGCTGGG CGCCCGAGCC AAAGCCCCGC CGTCCCTGCC GCACAGCGCC
    TCCCTGCGGA GCACCGGGAG CATGTCGGTG GCATCGGCGG CTCTGGTGGC CGAGCCCCTG
    CAGAACGGGA GCCGGTCCCG ACAGCAGTCC TCTTCCTCCA GAGAGAGCCC GGTCCCCAAA
    GTGAGAGCCA TCCAGAGGCA GCAGACCCAG CAGGTTCAGT CACCTGTGGA CTCCGCCACA
    ATGTCCCCAG TCGAGAGGAC AGCAGCCTGG GTTCTGAACA ATGGGCAGTA TGAAGAGGAC
    GTGGAAGAAA CTGAGCAAAA TCAGGACGAA GCCAAGCATG CTGAGAAGTA CGAACAGGAA
    ATCACCAAAC TGAAGGAGCG CCTGCGAGTG TCCAGCCGGC GACTCGAGGA GTATGAGCGC
    CGGTTGCTCG TGCAGGAGCA GCAGATGCAG AGGCTGCTGC TGGAGTACAA GGCCCGGCTG
    GAGGACAGCG AGGAGCGGCT GCGCCGGCAG CAGGAGGAGA AGGACAGCCA GATGAAGAGC
    ATCATCAGCA GACTAATGGC CGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAG
    GCAGTTATTG ATGCGAAGCA AAAAATAATC GATGCACAGG AAAAACGGAT CGTGTCGCTG
    GATTCGGCCA ACACCAGACT GATGAGCGCG CTGACCCAGG TGAAGGAACG GTACAGCATG
    CAGGTCCGAA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC GGAGAATGGT
    GAATTCAAAA ACAGCAGCTG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011982037.1
    CDS89..3952
    Translation

    Target ORF information:

    RefSeq Version XM_012126647.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012126647.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ACACCGACAC GACCACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA AGAGCAGCAG
    GCAGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCGCT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAATCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCTCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AATCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC TGTGGAATGT CTGGATCTTG GTAGAGGGGA GCCTGTATCA
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGAA TTAAATCACG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAACTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTAGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCTTTGTAT GAGTCAGATG AAAACTGTGA AGTCGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGCGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AAGACATCAG CGAGCGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CCCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AAGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT
    TCCTTCCTAC AGGCGACCGT GGCAAAATTG GGACCTCTCC CTCGTGTTCT TGCTGATATT
    ACTAAATCTC TGACTAATCC TACACCAATA CAACAGCAAC TGAGACGCTT CACTGAGCAT
    AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC
    CCTACTGACA GTGATTTGCA CAAACTAAAA TCCCCAAGCC AGGACAACAC AGACAGTTAT
    TTCAGAGGGA AAACATTATT GCTGGTCCAG CAAGCCTCCT CCCAGAGCAT GACTTACTCG
    GAAAAGGATG AAAAGGAAAG TAGCCTCCCC AATGGCCGCA GCATCTCCCT CATGGACCTT
    CAGGAGTCGC ACGCCGCCCA AGCGGAGCAT CCGTCTGTGA TGCTCGACGT GCCCATGCGC
    TTGACCGGAA GCCAGCTCTC CATAACTCAG GTGGCCAGCA TCAAGCAACT GCGGGAGACC
    CAGAGCACCC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCC TGCACCCAGC CTTGAACCAG
    CCAGGCAGCC TCCAGCCCCT GTCCTTCCAG AACCCTGTCT ATCACCTCAG TAACCCCATT
    CCCGCAATGC CAAAGGCCTC TGTGGACTCC AGTTTGGAGA ACTTAAGCAC TGCCAGTTCC
    AGGAGCCAAA GTAACAGTGA AGACTTCAAA CTCAGCGGAC CCAGCAACAG CAGCATGGAG
    GACTTCACCA AACGGAGCAC GCAGAGTGAG GACTTCTCCA GACGCCACAC CATGCCCGAC
    AGACACATAC CTCTCGCCCT GCCGCGGCAG AACAGCACGG GGCAGACCCA GCTCCGGAAG
    ATGGACCCAG CGGGGCTGGG CGCCCGAGCC AAAGCCCCGC CGTCCCTGCC GCACAGCGCC
    TCCCTGCGGA GCACCGGGAG CATGTCGGTG GCATCGGCGG CTCTGGTGGC CGAGCCCCTG
    CAGAACGGGA GCCGGTCCCG ACAGCAGTCC TCTTCCTCCA GAGAGAGCCC GGTCCCCAAA
    GTGAGAGCCA TCCAGAGGCA GCAGACCCAG CAGGTTCAGT CACCTGTGGA CTCCGCCACA
    ATGTCCCCAG TCGAGAGGAC AGCAGCCTGG GTTCTGAACA ATGGGCAGTA TGAAGAGGAC
    GTGGAAGAAA CTGAGCAAAA TCAGGACGAA GCCAAGCATG CTGAGAAGTA CGAACAGGAA
    ATCACCAAAC TGAAGGAGCG CCTGCGAGTG TCCAGCCGGC GACTCGAGGA GTATGAGCGC
    CGGTTGCTCG TGCAGGAGCA GCAGATGCAG AGGCTGCTGC TGGAGTACAA GGCCCGGCTG
    GAGGACAGCG AGGAGCGGCT GCGCCGGCAG CAGGAGGAGA AGGACAGCCA GATGAAGAGC
    ATCATCAGCA GACTAATGGC CGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAG
    GCAGTTATTG ATGCGAAGCA AAAAATAATC GATGCACAGG AAAAACGGAT CGTGTCGCTG
    GATTCGGCCA ACACCAGACT GATGAGCGCG CTGACCCAGG TGAAGGAACG GTACAGCATG
    CAGGTCCGAA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC GGAGAATGGT
    GAATTCAAAA ACAGCAGCTG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv11441
    Clone ID Related Accession (Same CDS sequence) XM_012126648.2
    Accession Version XM_012126648.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product ras GTPase-activating protein nGAP isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012126648.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ACACCGACAC GACCACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA AGAGCAGCAG
    GCAGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCGCT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAATCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCTCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AATCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC TGTGGAATGT CTGGATCTTG GTAGAGGGGA GCCTGTATCA
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGAA TTAAATCACG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAACTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTAGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCTTTGTAT GAGTCAGATG AAAACTGTGA AGTCGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGCGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AAGACATCAG CGAGCGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CCCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AAGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGCGACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CTAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA AACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGTCGCA CGCCGCCCAA
    GCGGAGCATC CGTCTGTGAT GCTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CCGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CTTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG ACGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCAGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCGGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TTCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAAAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGAGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTCGT GCAGGAGCAG
    CAGATGCAGA GGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCCGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG ACTAATGGCC
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCGAAGCAA
    AAAATAATCG ATGCACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA CACCAGACTG
    ATGAGCGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA TGGCATCTCC
    CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011982038.1
    CDS89..3931
    Translation

    Target ORF information:

    RefSeq Version XM_012126648.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012126648.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ACACCGACAC GACCACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA AGAGCAGCAG
    GCAGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCGCT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAATCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCTCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AATCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC TGTGGAATGT CTGGATCTTG GTAGAGGGGA GCCTGTATCA
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGAA TTAAATCACG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAACTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTAGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCTTTGTAT GAGTCAGATG AAAACTGTGA AGTCGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGCGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AAGACATCAG CGAGCGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CCCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AAGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGCGACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CTAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA AACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGTCGCA CGCCGCCCAA
    GCGGAGCATC CGTCTGTGAT GCTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CCGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CTTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG ACGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCAGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCGGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TTCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAAAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGAGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTCGT GCAGGAGCAG
    CAGATGCAGA GGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCCGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG ACTAATGGCC
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCGAAGCAA
    AAAATAATCG ATGCACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA CACCAGACTG
    ATGAGCGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA TGGCATCTCC
    CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv11442
    Clone ID Related Accession (Same CDS sequence) XM_012126646.2
    Accession Version XM_012126646.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3873bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product ras GTPase-activating protein nGAP isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012126646.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ACACCGACAC GACCACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA AGAGCAGCAG
    GCAGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCGCT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAATCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCTCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AATCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC TGTGGAATGT CTGGATCTTG GTAGAGGGGA GCCTGTATCA
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGAA TTAAATCACG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAACTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTAGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCTTTGTAT GAGTCAGATG AAAACTGTGA AGTCGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGCGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AAGACATCAG CGAGCGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CCCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AAGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT
    TCCTTCCTAC AGGCGACCGT GGCAAAATTG GGACCTCTCC CTCGTGTTCT TGCTGATATT
    ACTAAATCTC TGACTAATCC TACACCAATA CAACAGCAAC TGAGACGCTT CACTGAGCAT
    AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC
    CCTACTGACA GTGATTTGCA CAAACTAAAA TCCCCAAGCC AGGACAACAC AGACAGTTAT
    TTCAGAGGGA AAACATTATT GCTGGTCCAG CAAGCCTCCT CCCAGAGCAT GACTTACTCG
    GAAAAGGATG AAAAGGAAAG TAGCCTCCCC AATGGCCGCA GCATCTCCCT CATGGACCTT
    CAGGAGTCGC ACGCCGCCCA AGCGGAGCAT CCGTCTGTGA TGCTCGACGT GCCCATGCGC
    TTGACCGGAA GCCAGCTCTC CATAACTCAG GTGGCCAGCA TCAAGCAACT GCGGGAGACC
    CAGAGCACCC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCC TGCACCCAGC CTTGAACCAG
    CCAGGCAGCC TCCAGCCCCT GTCCTTCCAG AACCCTGTCT ATCACCTCAG TAACCCCATT
    CCCGCAATGC CAAAGGCCTC TGTGGACTCC AGTTTGGAGA ACTTAAGCAC TGCCAGTTCC
    AGGAGCCAAA GTAACAGTGA AGACTTCAAA CTCAGCGGAC CCAGCAACAG CAGCATGGAG
    GACTTCACCA AACGGAGCAC GCAGAGTGAG GACTTCTCCA GACGCCACAC CATGCCCGAC
    AGACACATAC CTCTCGCCCT GCCGCGGCAG AACAGCACGG GGCAGACCCA GCTCCGGAAG
    ATGGACCCAG CGGGGCTGGG CGCCCGAGCC AAAGCCCCGC CGTCCCTGCC GCACAGCGCC
    TCCCTGCGGA GCACCGGGAG CATGTCGGTG GCATCGGCGG CTCTGGTGGC CGAGCCCCTG
    CAGAACGGGA GCCGGTCCCG ACAGCAGTCC TCTTCCTCCA GAGAGAGCCC GGTCCCCAAA
    GTGAGAGCCA TCCAGAGGCA GCAGACCCAG CAGGTTCAGT CACCTGTGGA CTCCGCCACA
    ATGTCCCCAG TCGAGAGGAC AGCAGCCTGG GTTCTGAACA ATGGGCAGTA TGAAGAGGAC
    GTGGAAGAAA CTGAGCAAAA TCAGGACGAA GCCAAGCATG CTGAGAAGTA CGAACAGGAA
    ATCACCAAAC TGAAGGAGCG CCTGCGAGTG TCCAGCCGGC GACTCGAGGA GTATGAGCGC
    CGGTTGCTCG TGCAGGAGCA GCAGATGCAG AGGCTGCTGC TGGAGTACAA GGCCCGGCTG
    GAGGACAGCG AGGAGCGGCT GCGCCGGCAG CAGGAGGAGA AGGACAGCCA GATGAAGAGC
    ATCATCAGCA GACTAATGGC CGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAG
    GCAGTTATTG ATGCGAAGCA AAAAATAATC GATGCACAGG TCATAAATGG AGATGAGATT
    ATGCAAACAG GAAAAACGGA TCGTGTCGCT GGATTCGGCC AACACCAGAC TGATGAGCGC
    GCTGACCCAG GTGAAGGAAC GGTACAGCAT GCAGGTCCGA AATGGCATCT CCCCCACCAA
    CCCCACCAAG CTTTCCATCA CGGAGAATGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011982036.1
    CDS145..4017
    Translation

    Target ORF information:

    RefSeq Version XM_012126646.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012126646.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ACACCGACAC GACCACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA AGAGCAGCAG
    GCAGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCGCT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAATCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCTCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AATCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC TGTGGAATGT CTGGATCTTG GTAGAGGGGA GCCTGTATCA
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGAA TTAAATCACG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAACTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTAGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCTTTGTAT GAGTCAGATG AAAACTGTGA AGTCGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGCGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AAGACATCAG CGAGCGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CCCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AAGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT
    TCCTTCCTAC AGGCGACCGT GGCAAAATTG GGACCTCTCC CTCGTGTTCT TGCTGATATT
    ACTAAATCTC TGACTAATCC TACACCAATA CAACAGCAAC TGAGACGCTT CACTGAGCAT
    AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC
    CCTACTGACA GTGATTTGCA CAAACTAAAA TCCCCAAGCC AGGACAACAC AGACAGTTAT
    TTCAGAGGGA AAACATTATT GCTGGTCCAG CAAGCCTCCT CCCAGAGCAT GACTTACTCG
    GAAAAGGATG AAAAGGAAAG TAGCCTCCCC AATGGCCGCA GCATCTCCCT CATGGACCTT
    CAGGAGTCGC ACGCCGCCCA AGCGGAGCAT CCGTCTGTGA TGCTCGACGT GCCCATGCGC
    TTGACCGGAA GCCAGCTCTC CATAACTCAG GTGGCCAGCA TCAAGCAACT GCGGGAGACC
    CAGAGCACCC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCC TGCACCCAGC CTTGAACCAG
    CCAGGCAGCC TCCAGCCCCT GTCCTTCCAG AACCCTGTCT ATCACCTCAG TAACCCCATT
    CCCGCAATGC CAAAGGCCTC TGTGGACTCC AGTTTGGAGA ACTTAAGCAC TGCCAGTTCC
    AGGAGCCAAA GTAACAGTGA AGACTTCAAA CTCAGCGGAC CCAGCAACAG CAGCATGGAG
    GACTTCACCA AACGGAGCAC GCAGAGTGAG GACTTCTCCA GACGCCACAC CATGCCCGAC
    AGACACATAC CTCTCGCCCT GCCGCGGCAG AACAGCACGG GGCAGACCCA GCTCCGGAAG
    ATGGACCCAG CGGGGCTGGG CGCCCGAGCC AAAGCCCCGC CGTCCCTGCC GCACAGCGCC
    TCCCTGCGGA GCACCGGGAG CATGTCGGTG GCATCGGCGG CTCTGGTGGC CGAGCCCCTG
    CAGAACGGGA GCCGGTCCCG ACAGCAGTCC TCTTCCTCCA GAGAGAGCCC GGTCCCCAAA
    GTGAGAGCCA TCCAGAGGCA GCAGACCCAG CAGGTTCAGT CACCTGTGGA CTCCGCCACA
    ATGTCCCCAG TCGAGAGGAC AGCAGCCTGG GTTCTGAACA ATGGGCAGTA TGAAGAGGAC
    GTGGAAGAAA CTGAGCAAAA TCAGGACGAA GCCAAGCATG CTGAGAAGTA CGAACAGGAA
    ATCACCAAAC TGAAGGAGCG CCTGCGAGTG TCCAGCCGGC GACTCGAGGA GTATGAGCGC
    CGGTTGCTCG TGCAGGAGCA GCAGATGCAG AGGCTGCTGC TGGAGTACAA GGCCCGGCTG
    GAGGACAGCG AGGAGCGGCT GCGCCGGCAG CAGGAGGAGA AGGACAGCCA GATGAAGAGC
    ATCATCAGCA GACTAATGGC CGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAG
    GCAGTTATTG ATGCGAAGCA AAAAATAATC GATGCACAGG TCATAAATGG AGATGAGATT
    ATGCAAACAG GAAAAACGGA TCGTGTCGCT GGATTCGGCC AACACCAGAC TGATGAGCGC
    GCTGACCCAG GTGAAGGAAC GGTACAGCAT GCAGGTCCGA AATGGCATCT CCCCCACCAA
    CCCCACCAAG CTTTCCATCA CGGAGAATGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv11443
    Clone ID Related Accession (Same CDS sequence) XM_012126649.2
    Accession Version XM_012126649.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3438bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product ras GTPase-activating protein nGAP isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012126649.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGAAGCA GTTGTGATAA AGAGGTGCCA GCAGAAAGGT CCCCACGCAG ACGGAGTATC 
    TCAGGGACCA CTACATCTGA GAAACCCAAC TCTATGGACA CTGCGAATAC CTCACCCTTC
    AAAGTACCAG GGTTCTTCAG CAAGCGCCTA AAAGGCTCCA TCAAGAGGAC CAAAAGCCAA
    TCAAAGCTTG ACCGAAACAC GAGCTTTCGG CTTCCCTCTC TTCGCAATAC CGATGACAGG
    TCTCGTGGGC TGCCTAAACT AAAAGAATCC CGTTCTCATG AATCTTTGCT GAGCCCATGC
    AGTGCTGTGG AATGTCTGGA TCTTGGTAGA GGGGAGCCTG TATCAGTAAA GCCACTCCAT
    AGCAGCATCC TTGGACAAGA CTTCTGCTTT GAGGTTACCT ACTTAAGTGG AAGTAAATGC
    TTCAGTTGTA ACTCTGCTTC AGAGAGAGAT AAGTGGATGG AAAACCTTCG TAGGACAGTT
    CAACCAAATA AGGACAATTG TAGACGAGCT GAAAATGTTC TCCGTTTATG GATTATTGAA
    GCCAAGGACC TTGCCCCTAA AAAGAAATAT TTCTGTGAAC TGTGCCTTGA TGATACCCTC
    TTTGCTCGTA CAACCAGCAA GACCAAAGCA GACAATATTT TCTGGGGTGA ACATTTTGAG
    TTCTACAGCC TTCCACCTCT TCATAGCATC ACAGTTCACA TTTATAAAGA TGTGGAAAAA
    AAGAAGAAAA AAGACAAGAA TAATTATGTA GGGCTAGTCA ACATCCCCAC TGCCAGTGTG
    ACTGGTCGCC AGTTTGTAGA AAAGTGGTAC CCAGTGAGTA CACCTACACC CAACAAAGGA
    AAGACAGGAG GACCTTCTAT TCGAATTAAA TCACGTTTCC AAACCATCAC CATTCTGCCT
    ATGGAGCAAT ACAAAGAGTT TGCAGAATTT GTCACCAGCA ACTATACCAT GTTATGTTCT
    GTTCTTGAAC CAGTAATCAG TGTGAGAAAT AAAGAAGAAC TAGCCTGTGC CTTAGTGCAC
    ATTCTTCAAA GCACTGGAAG GGCCAAGGAT TTTCTGACGG ACTTGGTGAT GTCTGAGGTG
    GATCGTTGTG GAGAACATGA TGTCTTGATC TTCAGAGAGA ACACCATTGC CACCAAATCC
    ATTGAGGAAT ACCTCAAGTT AGTGGGACAA CAGTATCTTC ACGATGCACT GGGGGAGTTC
    ATCAAAGCTT TGTATGAGTC AGATGAAAAC TGTGAAGTCG ATCCCAGCAA ATGTTCATCC
    AGTGAACTTC TGGACCATCA GAGCAACCTG AAAATGTGCT GTGAGCTGGC TTTCTGCAAG
    ATCATCAACT CTTACTGTGT TTTTCCTCGC GAGCTGAAAG AAGTGTTTGC ATCCTGGAAG
    CAGCAGTGCC TGAACCGCGG CAAGCAAGAC ATCAGCGAGC GGCTCATCAG CGCCTCGCTG
    TTTCTCCGTT TTCTGTGCCC GGCCATCATG TCTCCCAGTC TCTTCAACCT CATGCAGGAG
    TATCCTGATG ACCGCACATC TCGGACCCTG ACTCTCATTG CCAAGGTCAT CCAGAACCTG
    GCCAACTTTG CCAAATTTGG TAACAAAGAG GAATACATGG CATTCATGAA TGATTTTTTA
    GAACATGAAT GGGGTGGAAT GAAGCGCTTT CTTTTGGAGA TCTCTAATCC AGACACCATC
    TCAAACACCC CAGGCTTTGA TGGTTACATT GATCTGGGCC GAGAGCTTTC AGTTTTGCAT
    TCCTTACTGT GGGAAGTAGT TTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCG
    ACCGTGGCAA AATTGGGACC TCTCCCTCGT GTTCTTGCTG ATATTACTAA ATCTCTGACT
    AATCCTACAC CAATACAACA GCAACTGAGA CGCTTCACTG AGCATAACTC CAGTCCAAAT
    GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG AAAATATTTG AAGACCCTAC TGACAGTGAT
    TTGCACAAAC TAAAATCCCC AAGCCAGGAC AACACAGACA GTTATTTCAG AGGGAAAACA
    TTATTGCTGG TCCAGCAAGC CTCCTCCCAG AGCATGACTT ACTCGGAAAA GGATGAAAAG
    GAAAGTAGCC TCCCCAATGG CCGCAGCATC TCCCTCATGG ACCTTCAGGA GTCGCACGCC
    GCCCAAGCGG AGCATCCGTC TGTGATGCTC GACGTGCCCA TGCGCTTGAC CGGAAGCCAG
    CTCTCCATAA CTCAGGTGGC CAGCATCAAG CAACTGCGGG AGACCCAGAG CACCCCCCAG
    AGTGCACCCC AAGTGCGAAG GCCCCTGCAC CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG
    CCCCTGTCCT TCCAGAACCC TGTCTATCAC CTCAGTAACC CCATTCCCGC AATGCCAAAG
    GCCTCTGTGG ACTCCAGTTT GGAGAACTTA AGCACTGCCA GTTCCAGGAG CCAAAGTAAC
    AGTGAAGACT TCAAACTCAG CGGACCCAGC AACAGCAGCA TGGAGGACTT CACCAAACGG
    AGCACGCAGA GTGAGGACTT CTCCAGACGC CACACCATGC CCGACAGACA CATACCTCTC
    GCCCTGCCGC GGCAGAACAG CACGGGGCAG ACCCAGCTCC GGAAGATGGA CCCAGCGGGG
    CTGGGCGCCC GAGCCAAAGC CCCGCCGTCC CTGCCGCACA GCGCCTCCCT GCGGAGCACC
    GGGAGCATGT CGGTGGCATC GGCGGCTCTG GTGGCCGAGC CCCTGCAGAA CGGGAGCCGG
    TCCCGACAGC AGTCCTCTTC CTCCAGAGAG AGCCCGGTCC CCAAAGTGAG AGCCATCCAG
    AGGCAGCAGA CCCAGCAGGT TCAGTCACCT GTGGACTCCG CCACAATGTC CCCAGTCGAG
    AGGACAGCAG CCTGGGTTCT GAACAATGGG CAGTATGAAG AGGACGTGGA AGAAACTGAG
    CAAAATCAGG ACGAAGCCAA GCATGCTGAG AAGTACGAAC AGGAAATCAC CAAACTGAAG
    GAGCGCCTGC GAGTGTCCAG CCGGCGACTC GAGGAGTATG AGCGCCGGTT GCTCGTGCAG
    GAGCAGCAGA TGCAGAGGCT GCTGCTGGAG TACAAGGCCC GGCTGGAGGA CAGCGAGGAG
    CGGCTGCGCC GGCAGCAGGA GGAGAAGGAC AGCCAGATGA AGAGCATCAT CAGCAGACTA
    ATGGCCGTGG AAGAGGAACT GAAGAAGGAT CATGCTGAGA TGCAGGCAGT TATTGATGCG
    AAGCAAAAAA TAATCGATGC ACAGGTCATA AATGGAGATG AGATTATGCA AACAGGAAAA
    ACGGATCGTG TCGCTGGATT CGGCCAACAC CAGACTGATG AGCGCGCTGA CCCAGGTGAA
    GGAACGGTAC AGCATGCAGG TCCGAAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC
    CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011982039.1
    CDS2990..6427
    Translation

    Target ORF information:

    RefSeq Version XM_012126649.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012126649.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGAAGCA GTTGTGATAA AGAGGTGCCA GCAGAAAGGT CCCCACGCAG ACGGAGTATC 
    TCAGGGACCA CTACATCTGA GAAACCCAAC TCTATGGACA CTGCGAATAC CTCACCCTTC
    AAAGTACCAG GGTTCTTCAG CAAGCGCCTA AAAGGCTCCA TCAAGAGGAC CAAAAGCCAA
    TCAAAGCTTG ACCGAAACAC GAGCTTTCGG CTTCCCTCTC TTCGCAATAC CGATGACAGG
    TCTCGTGGGC TGCCTAAACT AAAAGAATCC CGTTCTCATG AATCTTTGCT GAGCCCATGC
    AGTGCTGTGG AATGTCTGGA TCTTGGTAGA GGGGAGCCTG TATCAGTAAA GCCACTCCAT
    AGCAGCATCC TTGGACAAGA CTTCTGCTTT GAGGTTACCT ACTTAAGTGG AAGTAAATGC
    TTCAGTTGTA ACTCTGCTTC AGAGAGAGAT AAGTGGATGG AAAACCTTCG TAGGACAGTT
    CAACCAAATA AGGACAATTG TAGACGAGCT GAAAATGTTC TCCGTTTATG GATTATTGAA
    GCCAAGGACC TTGCCCCTAA AAAGAAATAT TTCTGTGAAC TGTGCCTTGA TGATACCCTC
    TTTGCTCGTA CAACCAGCAA GACCAAAGCA GACAATATTT TCTGGGGTGA ACATTTTGAG
    TTCTACAGCC TTCCACCTCT TCATAGCATC ACAGTTCACA TTTATAAAGA TGTGGAAAAA
    AAGAAGAAAA AAGACAAGAA TAATTATGTA GGGCTAGTCA ACATCCCCAC TGCCAGTGTG
    ACTGGTCGCC AGTTTGTAGA AAAGTGGTAC CCAGTGAGTA CACCTACACC CAACAAAGGA
    AAGACAGGAG GACCTTCTAT TCGAATTAAA TCACGTTTCC AAACCATCAC CATTCTGCCT
    ATGGAGCAAT ACAAAGAGTT TGCAGAATTT GTCACCAGCA ACTATACCAT GTTATGTTCT
    GTTCTTGAAC CAGTAATCAG TGTGAGAAAT AAAGAAGAAC TAGCCTGTGC CTTAGTGCAC
    ATTCTTCAAA GCACTGGAAG GGCCAAGGAT TTTCTGACGG ACTTGGTGAT GTCTGAGGTG
    GATCGTTGTG GAGAACATGA TGTCTTGATC TTCAGAGAGA ACACCATTGC CACCAAATCC
    ATTGAGGAAT ACCTCAAGTT AGTGGGACAA CAGTATCTTC ACGATGCACT GGGGGAGTTC
    ATCAAAGCTT TGTATGAGTC AGATGAAAAC TGTGAAGTCG ATCCCAGCAA ATGTTCATCC
    AGTGAACTTC TGGACCATCA GAGCAACCTG AAAATGTGCT GTGAGCTGGC TTTCTGCAAG
    ATCATCAACT CTTACTGTGT TTTTCCTCGC GAGCTGAAAG AAGTGTTTGC ATCCTGGAAG
    CAGCAGTGCC TGAACCGCGG CAAGCAAGAC ATCAGCGAGC GGCTCATCAG CGCCTCGCTG
    TTTCTCCGTT TTCTGTGCCC GGCCATCATG TCTCCCAGTC TCTTCAACCT CATGCAGGAG
    TATCCTGATG ACCGCACATC TCGGACCCTG ACTCTCATTG CCAAGGTCAT CCAGAACCTG
    GCCAACTTTG CCAAATTTGG TAACAAAGAG GAATACATGG CATTCATGAA TGATTTTTTA
    GAACATGAAT GGGGTGGAAT GAAGCGCTTT CTTTTGGAGA TCTCTAATCC AGACACCATC
    TCAAACACCC CAGGCTTTGA TGGTTACATT GATCTGGGCC GAGAGCTTTC AGTTTTGCAT
    TCCTTACTGT GGGAAGTAGT TTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCG
    ACCGTGGCAA AATTGGGACC TCTCCCTCGT GTTCTTGCTG ATATTACTAA ATCTCTGACT
    AATCCTACAC CAATACAACA GCAACTGAGA CGCTTCACTG AGCATAACTC CAGTCCAAAT
    GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG AAAATATTTG AAGACCCTAC TGACAGTGAT
    TTGCACAAAC TAAAATCCCC AAGCCAGGAC AACACAGACA GTTATTTCAG AGGGAAAACA
    TTATTGCTGG TCCAGCAAGC CTCCTCCCAG AGCATGACTT ACTCGGAAAA GGATGAAAAG
    GAAAGTAGCC TCCCCAATGG CCGCAGCATC TCCCTCATGG ACCTTCAGGA GTCGCACGCC
    GCCCAAGCGG AGCATCCGTC TGTGATGCTC GACGTGCCCA TGCGCTTGAC CGGAAGCCAG
    CTCTCCATAA CTCAGGTGGC CAGCATCAAG CAACTGCGGG AGACCCAGAG CACCCCCCAG
    AGTGCACCCC AAGTGCGAAG GCCCCTGCAC CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG
    CCCCTGTCCT TCCAGAACCC TGTCTATCAC CTCAGTAACC CCATTCCCGC AATGCCAAAG
    GCCTCTGTGG ACTCCAGTTT GGAGAACTTA AGCACTGCCA GTTCCAGGAG CCAAAGTAAC
    AGTGAAGACT TCAAACTCAG CGGACCCAGC AACAGCAGCA TGGAGGACTT CACCAAACGG
    AGCACGCAGA GTGAGGACTT CTCCAGACGC CACACCATGC CCGACAGACA CATACCTCTC
    GCCCTGCCGC GGCAGAACAG CACGGGGCAG ACCCAGCTCC GGAAGATGGA CCCAGCGGGG
    CTGGGCGCCC GAGCCAAAGC CCCGCCGTCC CTGCCGCACA GCGCCTCCCT GCGGAGCACC
    GGGAGCATGT CGGTGGCATC GGCGGCTCTG GTGGCCGAGC CCCTGCAGAA CGGGAGCCGG
    TCCCGACAGC AGTCCTCTTC CTCCAGAGAG AGCCCGGTCC CCAAAGTGAG AGCCATCCAG
    AGGCAGCAGA CCCAGCAGGT TCAGTCACCT GTGGACTCCG CCACAATGTC CCCAGTCGAG
    AGGACAGCAG CCTGGGTTCT GAACAATGGG CAGTATGAAG AGGACGTGGA AGAAACTGAG
    CAAAATCAGG ACGAAGCCAA GCATGCTGAG AAGTACGAAC AGGAAATCAC CAAACTGAAG
    GAGCGCCTGC GAGTGTCCAG CCGGCGACTC GAGGAGTATG AGCGCCGGTT GCTCGTGCAG
    GAGCAGCAGA TGCAGAGGCT GCTGCTGGAG TACAAGGCCC GGCTGGAGGA CAGCGAGGAG
    CGGCTGCGCC GGCAGCAGGA GGAGAAGGAC AGCCAGATGA AGAGCATCAT CAGCAGACTA
    ATGGCCGTGG AAGAGGAACT GAAGAAGGAT CATGCTGAGA TGCAGGCAGT TATTGATGCG
    AAGCAAAAAA TAATCGATGC ACAGGTCATA AATGGAGATG AGATTATGCA AACAGGAAAA
    ACGGATCGTG TCGCTGGATT CGGCCAACAC CAGACTGATG AGCGCGCTGA CCCAGGTGAA
    GGAACGGTAC AGCATGCAGG TCCGAAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC
    CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv11444
    Clone ID Related Accession (Same CDS sequence) XM_012126650.2
    Accession Version XM_012126650.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product ras GTPase-activating protein nGAP isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012126650.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGACCC CAGAGGTGCC AGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
    ACTACATCTG AGAAACCCAA CTCTATGGAC ACTGCGAATA CCTCACCCTT CAAAGTACCA
    GGGTTCTTCA GCAAGCGCCT AAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT
    GACCGAAACA CGAGCTTTCG GCTTCCCTCT CTTCGCAATA CCGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAATC CCGTTCTCAT GAATCTTTGC TGAGCCCATG CAGTGCTGTG
    GAATGTCTGG ATCTTGGTAG AGGGGAGCCT GTATCAGTAA AGCCACTCCA TAGCAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGTTGT
    AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTTC GTAGGACAGT TCAACCAAAT
    AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATTATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACCAGCA AGACCAAAGC AGACAATATT TTCTGGGGTG AACATTTTGA GTTCTACAGC
    CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAGAAA
    AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGAATTAA ATCACGTTTC CAAACCATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAGT TTGCAGAATT TGTCACCAGC AACTATACCA TGTTATGTTC TGTTCTTGAA
    CCAGTAATCA GTGTGAGAAA TAAAGAAGAA CTAGCCTGTG CCTTAGTGCA CATTCTTCAA
    AGCACTGGAA GGGCCAAGGA TTTTCTGACG GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAACATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TAGTGGGACA ACAGTATCTT CACGATGCAC TGGGGGAGTT CATCAAAGCT
    TTGTATGAGT CAGATGAAAA CTGTGAAGTC GATCCCAGCA AATGTTCATC CAGTGAACTT
    CTGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTTCCTCG CGAGCTGAAA GAAGTGTTTG CATCCTGGAA GCAGCAGTGC
    CTGAACCGCG GCAAGCAAGA CATCAGCGAG CGGCTCATCA GCGCCTCGCT GTTTCTCCGT
    TTTCTGTGCC CGGCCATCAT GTCTCCCAGT CTCTTCAACC TCATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACCCT GACTCTCATT GCCAAGGTCA TCCAGAACCT GGCCAACTTT
    GCCAAATTTG GTAACAAAGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA
    AAATTGGGAC CTCTCCCTCG TGTTCTTGCT GATATTACTA AATCTCTGAC TAATCCTACA
    CCAATACAAC AGCAACTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA
    AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCTA CTGACAGTGA TTTGCACAAA
    CTAAAATCCC CAAGCCAGGA CAACACAGAC AGTTATTTCA GAGGGAAAAC ATTATTGCTG
    GTCCAGCAAG CCTCCTCCCA GAGCATGACT TACTCGGAAA AGGATGAAAA GGAAAGTAGC
    CTCCCCAATG GCCGCAGCAT CTCCCTCATG GACCTTCAGG AGTCGCACGC CGCCCAAGCG
    GAGCATCCGT CTGTGATGCT CGACGTGCCC ATGCGCTTGA CCGGAAGCCA GCTCTCCATA
    ACTCAGGTGG CCAGCATCAA GCAACTGCGG GAGACCCAGA GCACCCCCCA GAGTGCACCC
    CAAGTGCGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCC
    TTCCAGAACC CTGTCTATCA CCTCAGTAAC CCCATTCCCG CAATGCCAAA GGCCTCTGTG
    GACTCCAGTT TGGAGAACTT AAGCACTGCC AGTTCCAGGA GCCAAAGTAA CAGTGAAGAC
    TTCAAACTCA GCGGACCCAG CAACAGCAGC ATGGAGGACT TCACCAAACG GAGCACGCAG
    AGTGAGGACT TCTCCAGACG CCACACCATG CCCGACAGAC ACATACCTCT CGCCCTGCCG
    CGGCAGAACA GCACGGGGCA GACCCAGCTC CGGAAGATGG ACCCAGCGGG GCTGGGCGCC
    CGAGCCAAAG CCCCGCCGTC CCTGCCGCAC AGCGCCTCCC TGCGGAGCAC CGGGAGCATG
    TCGGTGGCAT CGGCGGCTCT GGTGGCCGAG CCCCTGCAGA ACGGGAGCCG GTCCCGACAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCGGTC CCCAAAGTGA GAGCCATCCA GAGGCAGCAG
    ACCCAGCAGG TTCAGTCACC TGTGGACTCC GCCACAATGT CCCCAGTCGA GAGGACAGCA
    GCCTGGGTTC TGAACAATGG GCAGTATGAA GAGGACGTGG AAGAAACTGA GCAAAATCAG
    GACGAAGCCA AGCATGCTGA GAAGTACGAA CAGGAAATCA CCAAACTGAA GGAGCGCCTG
    CGAGTGTCCA GCCGGCGACT CGAGGAGTAT GAGCGCCGGT TGCTCGTGCA GGAGCAGCAG
    ATGCAGAGGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ACAGCGAGGA GCGGCTGCGC
    CGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAGAGCATCA TCAGCAGACT AATGGCCGTG
    GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAGGCAG TTATTGATGC GAAGCAAAAA
    ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATGC AAACAGGAAA AACGGATCGT
    GTCGCTGGAT TCGGCCAACA CCAGACTGAT GAGCGCGCTG ACCCAGGTGA AGGAACGGTA
    CAGCATGCAG GTCCGAAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011982040.1
    CDS207..3635
    Translation

    Target ORF information:

    RefSeq Version XM_012126650.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012126650.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGACCC CAGAGGTGCC AGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
    ACTACATCTG AGAAACCCAA CTCTATGGAC ACTGCGAATA CCTCACCCTT CAAAGTACCA
    GGGTTCTTCA GCAAGCGCCT AAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT
    GACCGAAACA CGAGCTTTCG GCTTCCCTCT CTTCGCAATA CCGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAATC CCGTTCTCAT GAATCTTTGC TGAGCCCATG CAGTGCTGTG
    GAATGTCTGG ATCTTGGTAG AGGGGAGCCT GTATCAGTAA AGCCACTCCA TAGCAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGTTGT
    AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTTC GTAGGACAGT TCAACCAAAT
    AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATTATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACCAGCA AGACCAAAGC AGACAATATT TTCTGGGGTG AACATTTTGA GTTCTACAGC
    CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAGAAA
    AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGAATTAA ATCACGTTTC CAAACCATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAGT TTGCAGAATT TGTCACCAGC AACTATACCA TGTTATGTTC TGTTCTTGAA
    CCAGTAATCA GTGTGAGAAA TAAAGAAGAA CTAGCCTGTG CCTTAGTGCA CATTCTTCAA
    AGCACTGGAA GGGCCAAGGA TTTTCTGACG GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAACATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TAGTGGGACA ACAGTATCTT CACGATGCAC TGGGGGAGTT CATCAAAGCT
    TTGTATGAGT CAGATGAAAA CTGTGAAGTC GATCCCAGCA AATGTTCATC CAGTGAACTT
    CTGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTTCCTCG CGAGCTGAAA GAAGTGTTTG CATCCTGGAA GCAGCAGTGC
    CTGAACCGCG GCAAGCAAGA CATCAGCGAG CGGCTCATCA GCGCCTCGCT GTTTCTCCGT
    TTTCTGTGCC CGGCCATCAT GTCTCCCAGT CTCTTCAACC TCATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACCCT GACTCTCATT GCCAAGGTCA TCCAGAACCT GGCCAACTTT
    GCCAAATTTG GTAACAAAGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA
    AAATTGGGAC CTCTCCCTCG TGTTCTTGCT GATATTACTA AATCTCTGAC TAATCCTACA
    CCAATACAAC AGCAACTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA
    AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCTA CTGACAGTGA TTTGCACAAA
    CTAAAATCCC CAAGCCAGGA CAACACAGAC AGTTATTTCA GAGGGAAAAC ATTATTGCTG
    GTCCAGCAAG CCTCCTCCCA GAGCATGACT TACTCGGAAA AGGATGAAAA GGAAAGTAGC
    CTCCCCAATG GCCGCAGCAT CTCCCTCATG GACCTTCAGG AGTCGCACGC CGCCCAAGCG
    GAGCATCCGT CTGTGATGCT CGACGTGCCC ATGCGCTTGA CCGGAAGCCA GCTCTCCATA
    ACTCAGGTGG CCAGCATCAA GCAACTGCGG GAGACCCAGA GCACCCCCCA GAGTGCACCC
    CAAGTGCGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCC
    TTCCAGAACC CTGTCTATCA CCTCAGTAAC CCCATTCCCG CAATGCCAAA GGCCTCTGTG
    GACTCCAGTT TGGAGAACTT AAGCACTGCC AGTTCCAGGA GCCAAAGTAA CAGTGAAGAC
    TTCAAACTCA GCGGACCCAG CAACAGCAGC ATGGAGGACT TCACCAAACG GAGCACGCAG
    AGTGAGGACT TCTCCAGACG CCACACCATG CCCGACAGAC ACATACCTCT CGCCCTGCCG
    CGGCAGAACA GCACGGGGCA GACCCAGCTC CGGAAGATGG ACCCAGCGGG GCTGGGCGCC
    CGAGCCAAAG CCCCGCCGTC CCTGCCGCAC AGCGCCTCCC TGCGGAGCAC CGGGAGCATG
    TCGGTGGCAT CGGCGGCTCT GGTGGCCGAG CCCCTGCAGA ACGGGAGCCG GTCCCGACAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCGGTC CCCAAAGTGA GAGCCATCCA GAGGCAGCAG
    ACCCAGCAGG TTCAGTCACC TGTGGACTCC GCCACAATGT CCCCAGTCGA GAGGACAGCA
    GCCTGGGTTC TGAACAATGG GCAGTATGAA GAGGACGTGG AAGAAACTGA GCAAAATCAG
    GACGAAGCCA AGCATGCTGA GAAGTACGAA CAGGAAATCA CCAAACTGAA GGAGCGCCTG
    CGAGTGTCCA GCCGGCGACT CGAGGAGTAT GAGCGCCGGT TGCTCGTGCA GGAGCAGCAG
    ATGCAGAGGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ACAGCGAGGA GCGGCTGCGC
    CGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAGAGCATCA TCAGCAGACT AATGGCCGTG
    GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAGGCAG TTATTGATGC GAAGCAAAAA
    ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATGC AAACAGGAAA AACGGATCGT
    GTCGCTGGAT TCGGCCAACA CCAGACTGAT GAGCGCGCTG ACCCAGGTGA AGGAACGGTA
    CAGCATGCAG GTCCGAAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm51966
    Clone ID Related Accession (Same CDS sequence) XM_015099332.1
    Accession Version XM_015099332.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3849bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries(sheep)
    Product LOW QUALITY PROTEIN: ras GTPase-activating protein nGAP isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639021.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCACCCT ACACCGACAC GACCACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA AGAGCAGCAG
    GCAGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCGCT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAATCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCCCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AATCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC TGTGGAATGT CTGGATCTTG GTAGAGGGGA GCCTGTATCA
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAATTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGAA TTAAATCACG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAACTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTAGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCTTTGTAT GAGTCAGATG AAAACTGTGA AGTCGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGCGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AAGACATCAG CGAGCGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CCCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AAGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGCGACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CTAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA AACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGTCGCA CGCCGCCCAA
    GCGGAGCATC CGTCTGTGAT GCTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CCGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CTTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG ACGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCAGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGGCGGA GACCCGCCGC
    GGAAGCATGT CGGTGGCATC GGCGGCTCTG GTGGCCGAGC CCCTGCAGAA CGGGAGCCGG
    TCCCGACAGC AGTCCTCTTC CTCCAGAGAG AGCCCGGTCC CCAAAGTGAG AGCCATCCAG
    AGGCAGCAGA CCCAGCAGGT TCAGTCACCT GTGGACTCCG CCACAATGTC CCCAGTCGAG
    AGGACAGCAG CCTGGGTTCT GAACAATGGG CAGTATGAAG AGGACGTGGA AGAAACTGAG
    CAAAATCAGG ACGAAGCCAA GCATGCTGAG AAGTACGAAC AGGAAATCAC CAAACTGAAG
    GAGCGCCTGC GAGTGTCCAG CCGGCGACTC GAGGAGTATG AGCGCCGGTT GCTCGTGCAG
    GAGCAGCAGA TGCAGAGGCT GCTGCTGGAG TACAAGGCCC GGCTGGAGGA CAGCGAGGAG
    CGGCTGCGCC GGCAGCAGGA GGAGAAGGAC AGCCAGATGA AGAGCATCAT CAGCAGACTA
    ATGGCCGTGG AAGAGGAACT GAAGAAGGAT CATGCTGAGA TGCAGGCAGT TATTGATGCG
    AAGCAAAAAA TAATCGATGC ACAGGAAAAA CGGATCGTGT CGCTGGATTC GGCCAACACC
    AGACTGATGA GCGCGCTGAC CCAGGTGAAG GAACGGTACA GCATGCAGGT CCGAAATGGC
    ATCTCCCCCA CCAACCCCAC CAAGCTTTCC ATCACGGAGA ATGGTGAATT CAAAAACAGC
    AGCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014954818.1
    CDS145..3993
    Translation

    Target ORF information:

    RefSeq Version XM_015099332.1
    Organism Ovis aries(sheep)
    Definition Ovis aries RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015099332.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCACCCT ACACCGACAC GACCACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA AGAGCAGCAG
    GCAGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCGCT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAATCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCCCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AATCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC TGTGGAATGT CTGGATCTTG GTAGAGGGGA GCCTGTATCA
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAATTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGAA TTAAATCACG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAACTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTAGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCTTTGTAT GAGTCAGATG AAAACTGTGA AGTCGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGCGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AAGACATCAG CGAGCGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CCCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AAGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGCGACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CTAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA AACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGTCGCA CGCCGCCCAA
    GCGGAGCATC CGTCTGTGAT GCTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CCGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CTTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG ACGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCAGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGGCGGA GACCCGCCGC
    GGAAGCATGT CGGTGGCATC GGCGGCTCTG GTGGCCGAGC CCCTGCAGAA CGGGAGCCGG
    TCCCGACAGC AGTCCTCTTC CTCCAGAGAG AGCCCGGTCC CCAAAGTGAG AGCCATCCAG
    AGGCAGCAGA CCCAGCAGGT TCAGTCACCT GTGGACTCCG CCACAATGTC CCCAGTCGAG
    AGGACAGCAG CCTGGGTTCT GAACAATGGG CAGTATGAAG AGGACGTGGA AGAAACTGAG
    CAAAATCAGG ACGAAGCCAA GCATGCTGAG AAGTACGAAC AGGAAATCAC CAAACTGAAG
    GAGCGCCTGC GAGTGTCCAG CCGGCGACTC GAGGAGTATG AGCGCCGGTT GCTCGTGCAG
    GAGCAGCAGA TGCAGAGGCT GCTGCTGGAG TACAAGGCCC GGCTGGAGGA CAGCGAGGAG
    CGGCTGCGCC GGCAGCAGGA GGAGAAGGAC AGCCAGATGA AGAGCATCAT CAGCAGACTA
    ATGGCCGTGG AAGAGGAACT GAAGAAGGAT CATGCTGAGA TGCAGGCAGT TATTGATGCG
    AAGCAAAAAA TAATCGATGC ACAGGAAAAA CGGATCGTGT CGCTGGATTC GGCCAACACC
    AGACTGATGA GCGCGCTGAC CCAGGTGAAG GAACGGTACA GCATGCAGGT CCGAAATGGC
    ATCTCCCCCA CCAACCCCAC CAAGCTTTCC ATCACGGAGA ATGGTGAATT CAAAAACAGC
    AGCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm61880
    Clone ID Related Accession (Same CDS sequence) XM_027975902.1
    Accession Version XM_027975902.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3909bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-05
    Organism Ovis aries(sheep)
    Product ras GTPase-activating protein nGAP isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGAAGC AGAACCTGGT AAACACTCTG TCACTTCGGG TTCCATTCAG CAGATCTCTT 
    TCAGAGCCAG AACCCCGCTC CAGTGTCAGA TTTGTTAGAC GGTGGCCGAC TTCAGTGGGG
    TCAGTGGAGT CTGAGCAGCA GGGATATTTC ATCCGTGGCG TTTTCTCTAC TCTTTCCAAT
    GGCTTCTCCA GGATGCTGAG TCTAAAAGGA GAGTCAACCA GTGAGCCAGT AAGAGAAGAT
    GTGAAAGGGC CACCTACCCA CCGTCTGTCT TGTGGCCAGT CACCCTACAC CGACACGACC
    ACATGGGAGC GGAAGTACTG CATCCTCACA GACAGCCAGC TGGTGTTGCT CAACAAGGAG
    AAGGAGATAC CTGTGGAGGG AGGACACGAG CAGCAGGCAG ATTCCACCAA AGGGCGATGC
    CTGCGGAGAA CTGTCAGTGT TCCTTCCGAG GGTCAGTTTC CTGAATACCC ACCAGAGGGC
    GCCGCTAAAC TGGAGGTGCC AGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC
    ACTACATCTG AGAAACCCAA CTCTATGGAC ACTGCGAATA CCTCACCCTT CAAAGTACCA
    GGGTTCTTCA GCAAGCGCCT AAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT
    GACCGAAACA CGAGCTTTCG GCTTCCCTCC CTTCGCAATA CCGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAATC CCGTTCTCAT GAATCTTTGC TGAGCCCATG CAGTGCTGTG
    GAATGTCTGG ATCTTGGTAG AGGGGAGCCT GTATCAGTAA AGCCACTCCA TAGCAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGTTGT
    AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTTC GTAGGACAGT TCAACCAAAT
    AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATTATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACCAGCA AGACCAAAGC AGACAATATT TTCTGGGGTG AACATTTTGA GTTCTACAGC
    CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAGAAA
    AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGAATTAA ATCACGTTTC CAAACCATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAGT TTGCAGAATT TGTCACCAGC AACTATACCA TGTTATGTTC TGTTCTTGAA
    CCAGTAATCA GTGTGAGAAA TAAAGAAGAA CTAGCCTGTG CCTTAGTGCA CATTCTTCAA
    AGCACTGGAA GGGCCAAGGA TTTTCTGACG GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAACATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TAGTGGGACA ACAGTATCTT CACGATGCAC TGGGGGAGTT CATCAAAGCT
    TTGTATGAGT CAGATGAAAA CTGTGAAGTC GATCCCAGCA AATGTTCATC CAGTGAACTT
    CTGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTTCCTCG CGAGCTGAAA GAAGTGTTTG CATCCTGGAA GCAGCAGTGC
    CTGAACCGCG GCAAGCAAGA CATCAGCGAG CGGCTCATCA GCGCCTCGCT GTTTCTCCGT
    TTTCTGTGCC CGGCCATCAT GTCTCCCAGT CTCTTCAACC TCATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACCCT GACTCTCATT GCCAAGGTCA TCCAGAACCT GGCCAACTTT
    GCCAAATTTG GTAACAAAGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA
    AAATTGGGAC CTCTCCCTCG TGTTCTTGCT GATATTACTA AATCTCTGAC TAATCCTACA
    CCAATACAAC AGCAACTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA
    AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCTA CTGACAGTGA TTTGCACAAA
    CTAAAATCCC CAAGCCAGGA CAACACAGAC AGTTATTTCA GAGGGAAAAC ATTATTGCTG
    GTCCAGCAAG CCTCCTCCCA GAGCATGACT TACTCGGAAA AGGATGAAAA GGAAAGTAGC
    CTCCCCAATG GCCGCAGCAT CTCCCTCATG GACCTTCAGG AGTCGCACGC CGCCCAAGCG
    GAGCATCCGT CTGTGATGCT CGACGTGCCC ATGCGCTTGA CCGGAAGCCA GCTCTCCATA
    ACTCAGGTGG CCAGCATCAA GCAACTGCGG GAGACCCAGA GCACCCCCCA GAGTGCACCC
    CAAGTGCGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCC
    TTCCAGAACC CTGTCTATCA CCTCAGTAAC CCCATTCCCG CAATGCCAAA GGCCTCTGTG
    GACTCCAGTT TGGAGAACTT AAGCACTGCC AGTTCCAGGA GCCAAAGTAA CAGTGAAGAC
    TTCAAACTCA GCGGACCCAG CAACAGCAGC ATGGAGGACT TCACCAAACG GAGCACGCAG
    AGTGAGGACT TCTCCAGACG CCACACCATG CCCGACAGAC ACATACCTCT CGCCCTGCCG
    CGGCAGAACA GCACGGGGCA GACCCAGCTC CGGAAGATGG ACCCAGCGGG GCTGGGCGCC
    CGAGCCAAAG CCCCGCCGTC CCTGCCGCAC AGCGCCTCCC TGCGGAGCAC CGGGAGCATG
    TCGGTGGCAT CGGCGGCTCT GGTGGCCGAG CCCCTGCAGA ACGGGAGCCG GTCCCGACAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCGGTC CCCAAAGTGA GAGCCATCCA GAGGCAGCAG
    ACCCAGCAGG TTCAGTCACC TGTGGACTCC GCCACAATGT CCCCAGTCGA GAGGACAGCA
    GCCTGGGTTC TGAACAATGG GCAGTATGAA GAGGACGTGG AAGAAACTGA GCAAAATCAG
    GACGAAGCCA AGCATGCTGA GAAGTACGAA CAGGAAATCA CCAAACTGAA GGAGCGCCTG
    CGAGTGTCCA GCCGGCGACT CGAGGAGTAT GAGCGCCGGT TGCTCGTGCA GGAGCAGCAG
    ATGCAGAGGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ACAGCGAGGA GCGGCTGCGC
    CGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAGAGCATCA TCAGCAGACT AATGGCCGTG
    GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAGGCAG TTATTGATGC GAAGCAAAAA
    ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATGC AAACAGGAAA AACGGATCGT
    GTCGCTGGAT TCGGCCAACA CCAGACTGAT GAGCGCGCTG ACCCAGGTGA AGGAACGGTA
    CAGCATGCAG GTCCGAAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027831703.1
    CDS813..4721
    Translation

    Target ORF information:

    RefSeq Version XM_027975902.1
    Organism Ovis aries(sheep)
    Definition Ovis aries RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_027975902.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGAAGC AGAACCTGGT AAACACTCTG TCACTTCGGG TTCCATTCAG CAGATCTCTT 
    TCAGAGCCAG AACCCCGCTC CAGTGTCAGA TTTGTTAGAC GGTGGCCGAC TTCAGTGGGG
    TCAGTGGAGT CTGAGCAGCA GGGATATTTC ATCCGTGGCG TTTTCTCTAC TCTTTCCAAT
    GGCTTCTCCA GGATGCTGAG TCTAAAAGGA GAGTCAACCA GTGAGCCAGT AAGAGAAGAT
    GTGAAAGGGC CACCTACCCA CCGTCTGTCT TGTGGCCAGT CACCCTACAC CGACACGACC
    ACATGGGAGC GGAAGTACTG CATCCTCACA GACAGCCAGC TGGTGTTGCT CAACAAGGAG
    AAGGAGATAC CTGTGGAGGG AGGACACGAG CAGCAGGCAG ATTCCACCAA AGGGCGATGC
    CTGCGGAGAA CTGTCAGTGT TCCTTCCGAG GGTCAGTTTC CTGAATACCC ACCAGAGGGC
    GCCGCTAAAC TGGAGGTGCC AGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC
    ACTACATCTG AGAAACCCAA CTCTATGGAC ACTGCGAATA CCTCACCCTT CAAAGTACCA
    GGGTTCTTCA GCAAGCGCCT AAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT
    GACCGAAACA CGAGCTTTCG GCTTCCCTCC CTTCGCAATA CCGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAATC CCGTTCTCAT GAATCTTTGC TGAGCCCATG CAGTGCTGTG
    GAATGTCTGG ATCTTGGTAG AGGGGAGCCT GTATCAGTAA AGCCACTCCA TAGCAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGTTGT
    AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTTC GTAGGACAGT TCAACCAAAT
    AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATTATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACCAGCA AGACCAAAGC AGACAATATT TTCTGGGGTG AACATTTTGA GTTCTACAGC
    CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAGAAA
    AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGAATTAA ATCACGTTTC CAAACCATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAGT TTGCAGAATT TGTCACCAGC AACTATACCA TGTTATGTTC TGTTCTTGAA
    CCAGTAATCA GTGTGAGAAA TAAAGAAGAA CTAGCCTGTG CCTTAGTGCA CATTCTTCAA
    AGCACTGGAA GGGCCAAGGA TTTTCTGACG GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAACATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TAGTGGGACA ACAGTATCTT CACGATGCAC TGGGGGAGTT CATCAAAGCT
    TTGTATGAGT CAGATGAAAA CTGTGAAGTC GATCCCAGCA AATGTTCATC CAGTGAACTT
    CTGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTTCCTCG CGAGCTGAAA GAAGTGTTTG CATCCTGGAA GCAGCAGTGC
    CTGAACCGCG GCAAGCAAGA CATCAGCGAG CGGCTCATCA GCGCCTCGCT GTTTCTCCGT
    TTTCTGTGCC CGGCCATCAT GTCTCCCAGT CTCTTCAACC TCATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACCCT GACTCTCATT GCCAAGGTCA TCCAGAACCT GGCCAACTTT
    GCCAAATTTG GTAACAAAGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA
    AAATTGGGAC CTCTCCCTCG TGTTCTTGCT GATATTACTA AATCTCTGAC TAATCCTACA
    CCAATACAAC AGCAACTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA
    AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCTA CTGACAGTGA TTTGCACAAA
    CTAAAATCCC CAAGCCAGGA CAACACAGAC AGTTATTTCA GAGGGAAAAC ATTATTGCTG
    GTCCAGCAAG CCTCCTCCCA GAGCATGACT TACTCGGAAA AGGATGAAAA GGAAAGTAGC
    CTCCCCAATG GCCGCAGCAT CTCCCTCATG GACCTTCAGG AGTCGCACGC CGCCCAAGCG
    GAGCATCCGT CTGTGATGCT CGACGTGCCC ATGCGCTTGA CCGGAAGCCA GCTCTCCATA
    ACTCAGGTGG CCAGCATCAA GCAACTGCGG GAGACCCAGA GCACCCCCCA GAGTGCACCC
    CAAGTGCGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCC
    TTCCAGAACC CTGTCTATCA CCTCAGTAAC CCCATTCCCG CAATGCCAAA GGCCTCTGTG
    GACTCCAGTT TGGAGAACTT AAGCACTGCC AGTTCCAGGA GCCAAAGTAA CAGTGAAGAC
    TTCAAACTCA GCGGACCCAG CAACAGCAGC ATGGAGGACT TCACCAAACG GAGCACGCAG
    AGTGAGGACT TCTCCAGACG CCACACCATG CCCGACAGAC ACATACCTCT CGCCCTGCCG
    CGGCAGAACA GCACGGGGCA GACCCAGCTC CGGAAGATGG ACCCAGCGGG GCTGGGCGCC
    CGAGCCAAAG CCCCGCCGTC CCTGCCGCAC AGCGCCTCCC TGCGGAGCAC CGGGAGCATG
    TCGGTGGCAT CGGCGGCTCT GGTGGCCGAG CCCCTGCAGA ACGGGAGCCG GTCCCGACAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCGGTC CCCAAAGTGA GAGCCATCCA GAGGCAGCAG
    ACCCAGCAGG TTCAGTCACC TGTGGACTCC GCCACAATGT CCCCAGTCGA GAGGACAGCA
    GCCTGGGTTC TGAACAATGG GCAGTATGAA GAGGACGTGG AAGAAACTGA GCAAAATCAG
    GACGAAGCCA AGCATGCTGA GAAGTACGAA CAGGAAATCA CCAAACTGAA GGAGCGCCTG
    CGAGTGTCCA GCCGGCGACT CGAGGAGTAT GAGCGCCGGT TGCTCGTGCA GGAGCAGCAG
    ATGCAGAGGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ACAGCGAGGA GCGGCTGCGC
    CGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAGAGCATCA TCAGCAGACT AATGGCCGTG
    GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAGGCAG TTATTGATGC GAAGCAAAAA
    ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATGC AAACAGGAAA AACGGATCGT
    GTCGCTGGAT TCGGCCAACA CCAGACTGAT GAGCGCGCTG ACCCAGGTGA AGGAACGGTA
    CAGCATGCAG GTCCGAAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm61881
    Clone ID Related Accession (Same CDS sequence) XM_027975903.1
    Accession Version XM_027975903.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3900bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-05
    Organism Ovis aries(sheep)
    Product ras GTPase-activating protein nGAP isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGAAGC AGAACCTGGT AAACACTCTG TCACTTCGGG TTCCATTCAG CAGATCTCTT 
    TCAGAGCCAG AACCCCGCTC CAGTGTCAGA TTTGTTAGAC GGTGGCCGAC TTCAGTGGGG
    TCAGTGGAGT CTGAGCAGCA GGGATATTTC ATCCGTGGCG TTTTCTCTAC TCTTTCCAAT
    GGCTTCTCCA GGATGCTGAG TCTAAAAGGA GAGTCAACCA GTGAGCCAGT AAGAGAAGAT
    GTGAAAGGGC CACCTACCCA CCGTCTGTCT TGTGGCCAGT CACCCTACAC CGACACGACC
    ACATGGGAGC GGAAGTACTG CATCCTCACA GACAGCCAGC TGGTGTTGCT CAACAAGGAG
    AAGGAGATAC CTGTGGAGGG AGGACACGAG CAGCAGGCAG ATTCCACCAA AGGGCGATGC
    CTGCGGAGAA CTGTCAGTGT TCCTTCCGAG GGTCAGTTTC CTGAATACCC ACCAGAGGGC
    GCCGCTAAAC TGGAGGTGCC AGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC
    ACTACATCTG AGAAACCCAA CTCTATGGAC ACTGCGAATA CCTCACCCTT CAAAGTACCA
    GGGTTCTTCA GCAAGCGCCT AAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT
    GACCGAAACA CGAGCTTTCG GCTTCCCTCC CTTCGCAATA CCGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAATC CCGTTCTCAT GAATCTTTGC TGAGCCCATG CAGTGCTGTG
    GAATGTCTGG ATCTTGGTAG AGGGGAGCCT GTATCAGTAA AGCCACTCCA TAGCAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGTTGT
    AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTTC GTAGGACAGT TCAACCAAAT
    AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATTATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACCAGCA AGACCAAAGC AGACAATATT TTCTGGGGTG AACATTTTGA GTTCTACAGC
    CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAGAAA
    AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGAATTAA ATCACGTTTC CAAACCATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAGT TTGCAGAATT TGTCACCAGC AACTATACCA TGTTATGTTC TGTTCTTGAA
    CCAGTAATCA GTGTGAGAAA TAAAGAAGAA CTAGCCTGTG CCTTAGTGCA CATTCTTCAA
    AGCACTGGAA GGGCCAAGGA TTTTCTGACG GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAACATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TAGTGGGACA ACAGTATCTT CACGATGCAC TGGGGGAGTT CATCAAAGCT
    TTGTATGAGT CAGATGAAAA CTGTGAAGTC GATCCCAGCA AATGTTCATC CAGTGAACTT
    CTGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTTCCTCG CGAGCTGAAA GAAGTGTTTG CATCCTGGAA GCAGCAGTGC
    CTGAACCGCG GCAAGCAAGA CATCAGCGAG CGGCTCATCA GCGCCTCGCT GTTTCTCCGT
    TTTCTGTGCC CGGCCATCAT GTCTCCCAGT CTCTTCAACC TCATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACCCT GACTCTCATT GCCAAGGTCA TCCAGAACCT GGCCAACTTT
    GCCAAATTTG GTAACAAAGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA
    AAATTGGGAC CTCTCCCTCG TGTTCTTGCT GATATTACTA AATCTCTGAC TAATCCTACA
    CCAATACAAC AGCAACTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA
    AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCTA CTGACAGTGA TTTGCACAAA
    CTAAAATCCC CAAGCCAGGA CAACACAGAC AGTTATTTCA GAGGGAAAAC ATTATTGCTG
    GTCCAGCAAG CCTCCTCCCA GAGCATGACT TACTCGGAAA AGGATGAAAA GGAAAGTAGC
    CTCCCCAATG GCCGCAGCAT CTCCCTCATG GACCTTCAGG AGTCGCACGC CGCCCAAGCG
    GAGCATCCGT CTGTGATGCT CGACGTGCCC ATGCGCTTGA CCGGAAGCCA GCTCTCCATA
    ACTCAGGTGG CCAGCATCAA GCAACTGCGG GAGACCCAGA GCACCCCCCA GAGTGCACCC
    CAAGTGCGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCC
    TTCCAGAACC CTGTCTATCA CCTCAGTAAC CCCATTCCCG CAATGCCAAA GGCCTCTGTG
    GACTCCAGTT TGGAGAACTT AAGCACTGCC AGTTCCAGGA GCCAAAGTAA CAGTGAAGAC
    TTCAAACTCA GCGGACCCAG CAACAGCAGC ATGGAGGACT TCACCAAACG GAGCACGCAG
    AGTGAGGACT TCTCCAGACG CCACACCATG CCCGACAGAC ACATACCTCT CGCCCTGCCG
    CGGCAGAACA GCACGGGGCA GACCCAGCTC CGGAAGATGG ACCCAGCGGG GCTGGGCGCC
    CGAGCCAAAG CCCCGCCGTC CCTGCCGCAC AGCGCCTCCC TGCGGAGCAC CGGGAGCATG
    TCGGTGGCAT CGGCGGCTCT GGTGGCCGAG CCCCTGCAGA ACGGGAGCCG GTCCCGACAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCGGTC CCCAAAGTGA GAGCCATCCA GAGGCAGCAG
    ACCCAGCAGG TTCAGTCACC TGTGGACTCC GCCACAATGT CCCCAGTCGA GAGGACAGCA
    GCCTGGGTTC TGAACAATGG GCAGTATGAA GAGGACGTGG AAGAAACTGA GCAAAATCAG
    GACGAAGCCA AGCATGCTGA GAAGTACGAA CAGGAAATCA CCAAACTGAA GGAGCGCCTG
    CGAGTGTCCA GCCGGCGACT CGAGGAGTAT GAGCGCCGGT TGCTCGTGCA GGAGCAGCAG
    ATGCAGAGGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ACAGCGAGGA GCGGCTGCGC
    CGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAGAGCATCA TCAGCAGACT AATGGCCGTG
    GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAGGCAG TTATTGATGC GAAGCAAAAA
    ATAATCGATG CACAGGAAAA ACGGATCGTG TCGCTGGATT CGGCCAACAC CAGACTGATG
    AGCGCGCTGA CCCAGGTGAA GGAACGGTAC AGCATGCAGG TCCGAAATGG CATCTCCCCC
    ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGAAT TCAAAAACAG CAGCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027831704.1
    CDS811..4710
    Translation

    Target ORF information:

    RefSeq Version XM_027975903.1
    Organism Ovis aries(sheep)
    Definition Ovis aries RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_027975903.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGAAGC AGAACCTGGT AAACACTCTG TCACTTCGGG TTCCATTCAG CAGATCTCTT 
    TCAGAGCCAG AACCCCGCTC CAGTGTCAGA TTTGTTAGAC GGTGGCCGAC TTCAGTGGGG
    TCAGTGGAGT CTGAGCAGCA GGGATATTTC ATCCGTGGCG TTTTCTCTAC TCTTTCCAAT
    GGCTTCTCCA GGATGCTGAG TCTAAAAGGA GAGTCAACCA GTGAGCCAGT AAGAGAAGAT
    GTGAAAGGGC CACCTACCCA CCGTCTGTCT TGTGGCCAGT CACCCTACAC CGACACGACC
    ACATGGGAGC GGAAGTACTG CATCCTCACA GACAGCCAGC TGGTGTTGCT CAACAAGGAG
    AAGGAGATAC CTGTGGAGGG AGGACACGAG CAGCAGGCAG ATTCCACCAA AGGGCGATGC
    CTGCGGAGAA CTGTCAGTGT TCCTTCCGAG GGTCAGTTTC CTGAATACCC ACCAGAGGGC
    GCCGCTAAAC TGGAGGTGCC AGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC
    ACTACATCTG AGAAACCCAA CTCTATGGAC ACTGCGAATA CCTCACCCTT CAAAGTACCA
    GGGTTCTTCA GCAAGCGCCT AAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT
    GACCGAAACA CGAGCTTTCG GCTTCCCTCC CTTCGCAATA CCGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAATC CCGTTCTCAT GAATCTTTGC TGAGCCCATG CAGTGCTGTG
    GAATGTCTGG ATCTTGGTAG AGGGGAGCCT GTATCAGTAA AGCCACTCCA TAGCAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGTTGT
    AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTTC GTAGGACAGT TCAACCAAAT
    AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATTATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACCAGCA AGACCAAAGC AGACAATATT TTCTGGGGTG AACATTTTGA GTTCTACAGC
    CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAGAAA
    AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGAATTAA ATCACGTTTC CAAACCATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAGT TTGCAGAATT TGTCACCAGC AACTATACCA TGTTATGTTC TGTTCTTGAA
    CCAGTAATCA GTGTGAGAAA TAAAGAAGAA CTAGCCTGTG CCTTAGTGCA CATTCTTCAA
    AGCACTGGAA GGGCCAAGGA TTTTCTGACG GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAACATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TAGTGGGACA ACAGTATCTT CACGATGCAC TGGGGGAGTT CATCAAAGCT
    TTGTATGAGT CAGATGAAAA CTGTGAAGTC GATCCCAGCA AATGTTCATC CAGTGAACTT
    CTGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTTCCTCG CGAGCTGAAA GAAGTGTTTG CATCCTGGAA GCAGCAGTGC
    CTGAACCGCG GCAAGCAAGA CATCAGCGAG CGGCTCATCA GCGCCTCGCT GTTTCTCCGT
    TTTCTGTGCC CGGCCATCAT GTCTCCCAGT CTCTTCAACC TCATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACCCT GACTCTCATT GCCAAGGTCA TCCAGAACCT GGCCAACTTT
    GCCAAATTTG GTAACAAAGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA
    AAATTGGGAC CTCTCCCTCG TGTTCTTGCT GATATTACTA AATCTCTGAC TAATCCTACA
    CCAATACAAC AGCAACTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA
    AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCTA CTGACAGTGA TTTGCACAAA
    CTAAAATCCC CAAGCCAGGA CAACACAGAC AGTTATTTCA GAGGGAAAAC ATTATTGCTG
    GTCCAGCAAG CCTCCTCCCA GAGCATGACT TACTCGGAAA AGGATGAAAA GGAAAGTAGC
    CTCCCCAATG GCCGCAGCAT CTCCCTCATG GACCTTCAGG AGTCGCACGC CGCCCAAGCG
    GAGCATCCGT CTGTGATGCT CGACGTGCCC ATGCGCTTGA CCGGAAGCCA GCTCTCCATA
    ACTCAGGTGG CCAGCATCAA GCAACTGCGG GAGACCCAGA GCACCCCCCA GAGTGCACCC
    CAAGTGCGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCC
    TTCCAGAACC CTGTCTATCA CCTCAGTAAC CCCATTCCCG CAATGCCAAA GGCCTCTGTG
    GACTCCAGTT TGGAGAACTT AAGCACTGCC AGTTCCAGGA GCCAAAGTAA CAGTGAAGAC
    TTCAAACTCA GCGGACCCAG CAACAGCAGC ATGGAGGACT TCACCAAACG GAGCACGCAG
    AGTGAGGACT TCTCCAGACG CCACACCATG CCCGACAGAC ACATACCTCT CGCCCTGCCG
    CGGCAGAACA GCACGGGGCA GACCCAGCTC CGGAAGATGG ACCCAGCGGG GCTGGGCGCC
    CGAGCCAAAG CCCCGCCGTC CCTGCCGCAC AGCGCCTCCC TGCGGAGCAC CGGGAGCATG
    TCGGTGGCAT CGGCGGCTCT GGTGGCCGAG CCCCTGCAGA ACGGGAGCCG GTCCCGACAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCGGTC CCCAAAGTGA GAGCCATCCA GAGGCAGCAG
    ACCCAGCAGG TTCAGTCACC TGTGGACTCC GCCACAATGT CCCCAGTCGA GAGGACAGCA
    GCCTGGGTTC TGAACAATGG GCAGTATGAA GAGGACGTGG AAGAAACTGA GCAAAATCAG
    GACGAAGCCA AGCATGCTGA GAAGTACGAA CAGGAAATCA CCAAACTGAA GGAGCGCCTG
    CGAGTGTCCA GCCGGCGACT CGAGGAGTAT GAGCGCCGGT TGCTCGTGCA GGAGCAGCAG
    ATGCAGAGGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ACAGCGAGGA GCGGCTGCGC
    CGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAGAGCATCA TCAGCAGACT AATGGCCGTG
    GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAGGCAG TTATTGATGC GAAGCAAAAA
    ATAATCGATG CACAGGAAAA ACGGATCGTG TCGCTGGATT CGGCCAACAC CAGACTGATG
    AGCGCGCTGA CCCAGGTGAA GGAACGGTAC AGCATGCAGG TCCGAAATGG CATCTCCCCC
    ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGAAT TCAAAAACAG CAGCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm61882
    Clone ID Related Accession (Same CDS sequence) XM_027975904.1
    Accession Version XM_027975904.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-05
    Organism Ovis aries(sheep)
    Product ras GTPase-activating protein nGAP isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGAAGC AGAACCTGGT AAACACTCTG TCACTTCGGG TTCCATTCAG CAGATCTCTT 
    TCAGAGCCAG AACCCCGCTC CAGTGTCAGA TTTGTTAGAC GGTGGCCGAC TTCAGTGGGG
    TCAGTGGAGT CTGAGCAGCA GGGATATTTC ATCCGTGGCG TTTTCTCTAC TCTTTCCAAT
    GGCTTCTCCA GGATGCTGAG TCTAAAAGGA GAGTCAACCA GTGAGCCAGT AAGAGAAGAT
    GTGAAAGGGC CACCTACCCA CCGTCTGTCT TGTGGCCAGT CACCCTACAC CGACACGACC
    ACATGGGAGC GGAAGTACTG CATCCTCACA GACAGCCAGC TGGTGTTGCT CAACAAGGAG
    AAGGAGATAC CTGTGGAGGG AGGACACGAG CAGCAGGCAG ATTCCACCAA AGGGCGATGC
    CTGCGGAGAA CTGTCAGTGT TCCTTCCGAG GGTCAGTTTC CTGAATACCC ACCAGAGGGC
    GCCGCTAAAC TGGAGGTGCC AGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC
    ACTACATCTG AGAAACCCAA CTCTATGGAC ACTGCGAATA CCTCACCCTT CAAAGTACCA
    GGGTTCTTCA GCAAGCGCCT AAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT
    GACCGAAACA CGAGCTTTCG GCTTCCCTCC CTTCGCAATA CCGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAATC CCGTTCTCAT GAATCTTTGC TGAGCCCATG CAGTGCTGTG
    GAATGTCTGG ATCTTGGTAG AGGGGAGCCT GTATCAGTAA AGCCACTCCA TAGCAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGTTGT
    AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTTC GTAGGACAGT TCAACCAAAT
    AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATTATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACCAGCA AGACCAAAGC AGACAATATT TTCTGGGGTG AACATTTTGA GTTCTACAGC
    CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAGAAA
    AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGAATTAA ATCACGTTTC CAAACCATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAGT TTGCAGAATT TGTCACCAGC AACTATACCA TGTTATGTTC TGTTCTTGAA
    CCAGTAATCA GTGTGAGAAA TAAAGAAGAA CTAGCCTGTG CCTTAGTGCA CATTCTTCAA
    AGCACTGGAA GGGCCAAGGA TTTTCTGACG GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAACATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TAGTGGGACA ACAGTATCTT CACGATGCAC TGGGGGAGTT CATCAAAGCT
    TTGTATGAGT CAGATGAAAA CTGTGAAGTC GATCCCAGCA AATGTTCATC CAGTGAACTT
    CTGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTTCCTCG CGAGCTGAAA GAAGTGTTTG CATCCTGGAA GCAGCAGTGC
    CTGAACCGCG GCAAGCAAGA CATCAGCGAG CGGCTCATCA GCGCCTCGCT GTTTCTCCGT
    TTTCTGTGCC CGGCCATCAT GTCTCCCAGT CTCTTCAACC TCATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACCCT GACTCTCATT GCCAAGGTCA TCCAGAACCT GGCCAACTTT
    GCCAAATTTG GTAACAAAGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG
    TGGGAAGTAG TTTCCCAACT TGATAAGGCG ACCGTGGCAA AATTGGGACC TCTCCCTCGT
    GTTCTTGCTG ATATTACTAA ATCTCTGACT AATCCTACAC CAATACAACA GCAACTGAGA
    CGCTTCACTG AGCATAACTC CAGTCCAAAT GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG
    AAAATATTTG AAGACCCTAC TGACAGTGAT TTGCACAAAC TAAAATCCCC AAGCCAGGAC
    AACACAGACA GTTATTTCAG AGGGAAAACA TTATTGCTGG TCCAGCAAGC CTCCTCCCAG
    AGCATGACTT ACTCGGAAAA GGATGAAAAG GAAAGTAGCC TCCCCAATGG CCGCAGCATC
    TCCCTCATGG ACCTTCAGGA GTCGCACGCC GCCCAAGCGG AGCATCCGTC TGTGATGCTC
    GACGTGCCCA TGCGCTTGAC CGGAAGCCAG CTCTCCATAA CTCAGGTGGC CAGCATCAAG
    CAACTGCGGG AGACCCAGAG CACCCCCCAG AGTGCACCCC AAGTGCGAAG GCCCCTGCAC
    CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG CCCCTGTCCT TCCAGAACCC TGTCTATCAC
    CTCAGTAACC CCATTCCCGC AATGCCAAAG GCCTCTGTGG ACTCCAGTTT GGAGAACTTA
    AGCACTGCCA GTTCCAGGAG CCAAAGTAAC AGTGAAGACT TCAAACTCAG CGGACCCAGC
    AACAGCAGCA TGGAGGACTT CACCAAACGG AGCACGCAGA GTGAGGACTT CTCCAGACGC
    CACACCATGC CCGACAGACA CATACCTCTC GCCCTGCCGC GGCAGAACAG CACGGGGCAG
    ACCCAGCTCC GGAAGATGGA CCCAGCGGGG CTGGGCGCCC GAGCCAAAGC CCCGCCGTCC
    CTGCCGCACA GCGCCTCCCT GCGGAGCACC GGGAGCATGT CGGTGGCATC GGCGGCTCTG
    GTGGCCGAGC CCCTGCAGAA CGGGAGCCGG TCCCGACAGC AGTCCTCTTC CTCCAGAGAG
    AGCCCGGTCC CCAAAGTGAG AGCCATCCAG AGGCAGCAGA CCCAGCAGGT TCAGTCACCT
    GTGGACTCCG CCACAATGTC CCCAGTCGAG AGGACAGCAG CCTGGGTTCT GAACAATGGG
    CAGTATGAAG AGGACGTGGA AGAAACTGAG CAAAATCAGG ACGAAGCCAA GCATGCTGAG
    AAGTACGAAC AGGAAATCAC CAAACTGAAG GAGCGCCTGC GAGTGTCCAG CCGGCGACTC
    GAGGAGTATG AGCGCCGGTT GCTCGTGCAG GAGCAGCAGA TGCAGAGGCT GCTGCTGGAG
    TACAAGGCCC GGCTGGAGGA CAGCGAGGAG CGGCTGCGCC GGCAGCAGGA GGAGAAGGAC
    AGCCAGATGA AGAGCATCAT CAGCAGACTA ATGGCCGTGG AAGAGGAACT GAAGAAGGAT
    CATGCTGAGA TGCAGGCAGT TATTGATGCG AAGCAAAAAA TAATCGATGC ACAGGTCATA
    AATGGAGATG AGATTATGCA AACAGGAAAA ACGGATCGTG TCGCTGGATT CGGCCAACAC
    CAGACTGATG AGCGCGCTGA CCCAGGTGAA GGAACGGTAC AGCATGCAGG TCCGAAATGG
    CATCTCCCCC ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027831705.1
    CDS813..4700
    Translation

    Target ORF information:

    RefSeq Version XM_027975904.1
    Organism Ovis aries(sheep)
    Definition Ovis aries RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_027975904.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGAAGC AGAACCTGGT AAACACTCTG TCACTTCGGG TTCCATTCAG CAGATCTCTT 
    TCAGAGCCAG AACCCCGCTC CAGTGTCAGA TTTGTTAGAC GGTGGCCGAC TTCAGTGGGG
    TCAGTGGAGT CTGAGCAGCA GGGATATTTC ATCCGTGGCG TTTTCTCTAC TCTTTCCAAT
    GGCTTCTCCA GGATGCTGAG TCTAAAAGGA GAGTCAACCA GTGAGCCAGT AAGAGAAGAT
    GTGAAAGGGC CACCTACCCA CCGTCTGTCT TGTGGCCAGT CACCCTACAC CGACACGACC
    ACATGGGAGC GGAAGTACTG CATCCTCACA GACAGCCAGC TGGTGTTGCT CAACAAGGAG
    AAGGAGATAC CTGTGGAGGG AGGACACGAG CAGCAGGCAG ATTCCACCAA AGGGCGATGC
    CTGCGGAGAA CTGTCAGTGT TCCTTCCGAG GGTCAGTTTC CTGAATACCC ACCAGAGGGC
    GCCGCTAAAC TGGAGGTGCC AGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC
    ACTACATCTG AGAAACCCAA CTCTATGGAC ACTGCGAATA CCTCACCCTT CAAAGTACCA
    GGGTTCTTCA GCAAGCGCCT AAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT
    GACCGAAACA CGAGCTTTCG GCTTCCCTCC CTTCGCAATA CCGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAATC CCGTTCTCAT GAATCTTTGC TGAGCCCATG CAGTGCTGTG
    GAATGTCTGG ATCTTGGTAG AGGGGAGCCT GTATCAGTAA AGCCACTCCA TAGCAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGTTGT
    AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTTC GTAGGACAGT TCAACCAAAT
    AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATTATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACCAGCA AGACCAAAGC AGACAATATT TTCTGGGGTG AACATTTTGA GTTCTACAGC
    CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAGAAA
    AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGAATTAA ATCACGTTTC CAAACCATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAGT TTGCAGAATT TGTCACCAGC AACTATACCA TGTTATGTTC TGTTCTTGAA
    CCAGTAATCA GTGTGAGAAA TAAAGAAGAA CTAGCCTGTG CCTTAGTGCA CATTCTTCAA
    AGCACTGGAA GGGCCAAGGA TTTTCTGACG GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAACATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TAGTGGGACA ACAGTATCTT CACGATGCAC TGGGGGAGTT CATCAAAGCT
    TTGTATGAGT CAGATGAAAA CTGTGAAGTC GATCCCAGCA AATGTTCATC CAGTGAACTT
    CTGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTTCCTCG CGAGCTGAAA GAAGTGTTTG CATCCTGGAA GCAGCAGTGC
    CTGAACCGCG GCAAGCAAGA CATCAGCGAG CGGCTCATCA GCGCCTCGCT GTTTCTCCGT
    TTTCTGTGCC CGGCCATCAT GTCTCCCAGT CTCTTCAACC TCATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACCCT GACTCTCATT GCCAAGGTCA TCCAGAACCT GGCCAACTTT
    GCCAAATTTG GTAACAAAGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG
    TGGGAAGTAG TTTCCCAACT TGATAAGGCG ACCGTGGCAA AATTGGGACC TCTCCCTCGT
    GTTCTTGCTG ATATTACTAA ATCTCTGACT AATCCTACAC CAATACAACA GCAACTGAGA
    CGCTTCACTG AGCATAACTC CAGTCCAAAT GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG
    AAAATATTTG AAGACCCTAC TGACAGTGAT TTGCACAAAC TAAAATCCCC AAGCCAGGAC
    AACACAGACA GTTATTTCAG AGGGAAAACA TTATTGCTGG TCCAGCAAGC CTCCTCCCAG
    AGCATGACTT ACTCGGAAAA GGATGAAAAG GAAAGTAGCC TCCCCAATGG CCGCAGCATC
    TCCCTCATGG ACCTTCAGGA GTCGCACGCC GCCCAAGCGG AGCATCCGTC TGTGATGCTC
    GACGTGCCCA TGCGCTTGAC CGGAAGCCAG CTCTCCATAA CTCAGGTGGC CAGCATCAAG
    CAACTGCGGG AGACCCAGAG CACCCCCCAG AGTGCACCCC AAGTGCGAAG GCCCCTGCAC
    CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG CCCCTGTCCT TCCAGAACCC TGTCTATCAC
    CTCAGTAACC CCATTCCCGC AATGCCAAAG GCCTCTGTGG ACTCCAGTTT GGAGAACTTA
    AGCACTGCCA GTTCCAGGAG CCAAAGTAAC AGTGAAGACT TCAAACTCAG CGGACCCAGC
    AACAGCAGCA TGGAGGACTT CACCAAACGG AGCACGCAGA GTGAGGACTT CTCCAGACGC
    CACACCATGC CCGACAGACA CATACCTCTC GCCCTGCCGC GGCAGAACAG CACGGGGCAG
    ACCCAGCTCC GGAAGATGGA CCCAGCGGGG CTGGGCGCCC GAGCCAAAGC CCCGCCGTCC
    CTGCCGCACA GCGCCTCCCT GCGGAGCACC GGGAGCATGT CGGTGGCATC GGCGGCTCTG
    GTGGCCGAGC CCCTGCAGAA CGGGAGCCGG TCCCGACAGC AGTCCTCTTC CTCCAGAGAG
    AGCCCGGTCC CCAAAGTGAG AGCCATCCAG AGGCAGCAGA CCCAGCAGGT TCAGTCACCT
    GTGGACTCCG CCACAATGTC CCCAGTCGAG AGGACAGCAG CCTGGGTTCT GAACAATGGG
    CAGTATGAAG AGGACGTGGA AGAAACTGAG CAAAATCAGG ACGAAGCCAA GCATGCTGAG
    AAGTACGAAC AGGAAATCAC CAAACTGAAG GAGCGCCTGC GAGTGTCCAG CCGGCGACTC
    GAGGAGTATG AGCGCCGGTT GCTCGTGCAG GAGCAGCAGA TGCAGAGGCT GCTGCTGGAG
    TACAAGGCCC GGCTGGAGGA CAGCGAGGAG CGGCTGCGCC GGCAGCAGGA GGAGAAGGAC
    AGCCAGATGA AGAGCATCAT CAGCAGACTA ATGGCCGTGG AAGAGGAACT GAAGAAGGAT
    CATGCTGAGA TGCAGGCAGT TATTGATGCG AAGCAAAAAA TAATCGATGC ACAGGTCATA
    AATGGAGATG AGATTATGCA AACAGGAAAA ACGGATCGTG TCGCTGGATT CGGCCAACAC
    CAGACTGATG AGCGCGCTGA CCCAGGTGAA GGAACGGTAC AGCATGCAGG TCCGAAATGG
    CATCTCCCCC ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm61883
    Clone ID Related Accession (Same CDS sequence) XM_027975905.1
    Accession Version XM_027975905.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3879bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-05
    Organism Ovis aries(sheep)
    Product ras GTPase-activating protein nGAP isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGAAGC AGAACCTGGT AAACACTCTG TCACTTCGGG TTCCATTCAG CAGATCTCTT 
    TCAGAGCCAG AACCCCGCTC CAGTGTCAGA TTTGTTAGAC GGTGGCCGAC TTCAGTGGGG
    TCAGTGGAGT CTGAGCAGCA GGGATATTTC ATCCGTGGCG TTTTCTCTAC TCTTTCCAAT
    GGCTTCTCCA GGATGCTGAG TCTAAAAGGA GAGTCAACCA GTGAGCCAGT AAGAGAAGAT
    GTGAAAGGGC CACCTACCCA CCGTCTGTCT TGTGGCCAGT CACCCTACAC CGACACGACC
    ACATGGGAGC GGAAGTACTG CATCCTCACA GACAGCCAGC TGGTGTTGCT CAACAAGGAG
    AAGGAGATAC CTGTGGAGGG AGGACACGAG CAGCAGGCAG ATTCCACCAA AGGGCGATGC
    CTGCGGAGAA CTGTCAGTGT TCCTTCCGAG GGTCAGTTTC CTGAATACCC ACCAGAGGGC
    GCCGCTAAAC TGGAGGTGCC AGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC
    ACTACATCTG AGAAACCCAA CTCTATGGAC ACTGCGAATA CCTCACCCTT CAAAGTACCA
    GGGTTCTTCA GCAAGCGCCT AAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT
    GACCGAAACA CGAGCTTTCG GCTTCCCTCC CTTCGCAATA CCGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAATC CCGTTCTCAT GAATCTTTGC TGAGCCCATG CAGTGCTGTG
    GAATGTCTGG ATCTTGGTAG AGGGGAGCCT GTATCAGTAA AGCCACTCCA TAGCAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGTTGT
    AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTTC GTAGGACAGT TCAACCAAAT
    AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATTATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACCAGCA AGACCAAAGC AGACAATATT TTCTGGGGTG AACATTTTGA GTTCTACAGC
    CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAGAAA
    AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGAATTAA ATCACGTTTC CAAACCATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAGT TTGCAGAATT TGTCACCAGC AACTATACCA TGTTATGTTC TGTTCTTGAA
    CCAGTAATCA GTGTGAGAAA TAAAGAAGAA CTAGCCTGTG CCTTAGTGCA CATTCTTCAA
    AGCACTGGAA GGGCCAAGGA TTTTCTGACG GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAACATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TAGTGGGACA ACAGTATCTT CACGATGCAC TGGGGGAGTT CATCAAAGCT
    TTGTATGAGT CAGATGAAAA CTGTGAAGTC GATCCCAGCA AATGTTCATC CAGTGAACTT
    CTGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTTCCTCG CGAGCTGAAA GAAGTGTTTG CATCCTGGAA GCAGCAGTGC
    CTGAACCGCG GCAAGCAAGA CATCAGCGAG CGGCTCATCA GCGCCTCGCT GTTTCTCCGT
    TTTCTGTGCC CGGCCATCAT GTCTCCCAGT CTCTTCAACC TCATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACCCT GACTCTCATT GCCAAGGTCA TCCAGAACCT GGCCAACTTT
    GCCAAATTTG GTAACAAAGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG
    TGGGAAGTAG TTTCCCAACT TGATAAGGCG ACCGTGGCAA AATTGGGACC TCTCCCTCGT
    GTTCTTGCTG ATATTACTAA ATCTCTGACT AATCCTACAC CAATACAACA GCAACTGAGA
    CGCTTCACTG AGCATAACTC CAGTCCAAAT GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG
    AAAATATTTG AAGACCCTAC TGACAGTGAT TTGCACAAAC TAAAATCCCC AAGCCAGGAC
    AACACAGACA GTTATTTCAG AGGGAAAACA TTATTGCTGG TCCAGCAAGC CTCCTCCCAG
    AGCATGACTT ACTCGGAAAA GGATGAAAAG GAAAGTAGCC TCCCCAATGG CCGCAGCATC
    TCCCTCATGG ACCTTCAGGA GTCGCACGCC GCCCAAGCGG AGCATCCGTC TGTGATGCTC
    GACGTGCCCA TGCGCTTGAC CGGAAGCCAG CTCTCCATAA CTCAGGTGGC CAGCATCAAG
    CAACTGCGGG AGACCCAGAG CACCCCCCAG AGTGCACCCC AAGTGCGAAG GCCCCTGCAC
    CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG CCCCTGTCCT TCCAGAACCC TGTCTATCAC
    CTCAGTAACC CCATTCCCGC AATGCCAAAG GCCTCTGTGG ACTCCAGTTT GGAGAACTTA
    AGCACTGCCA GTTCCAGGAG CCAAAGTAAC AGTGAAGACT TCAAACTCAG CGGACCCAGC
    AACAGCAGCA TGGAGGACTT CACCAAACGG AGCACGCAGA GTGAGGACTT CTCCAGACGC
    CACACCATGC CCGACAGACA CATACCTCTC GCCCTGCCGC GGCAGAACAG CACGGGGCAG
    ACCCAGCTCC GGAAGATGGA CCCAGCGGGG CTGGGCGCCC GAGCCAAAGC CCCGCCGTCC
    CTGCCGCACA GCGCCTCCCT GCGGAGCACC GGGAGCATGT CGGTGGCATC GGCGGCTCTG
    GTGGCCGAGC CCCTGCAGAA CGGGAGCCGG TCCCGACAGC AGTCCTCTTC CTCCAGAGAG
    AGCCCGGTCC CCAAAGTGAG AGCCATCCAG AGGCAGCAGA CCCAGCAGGT TCAGTCACCT
    GTGGACTCCG CCACAATGTC CCCAGTCGAG AGGACAGCAG CCTGGGTTCT GAACAATGGG
    CAGTATGAAG AGGACGTGGA AGAAACTGAG CAAAATCAGG ACGAAGCCAA GCATGCTGAG
    AAGTACGAAC AGGAAATCAC CAAACTGAAG GAGCGCCTGC GAGTGTCCAG CCGGCGACTC
    GAGGAGTATG AGCGCCGGTT GCTCGTGCAG GAGCAGCAGA TGCAGAGGCT GCTGCTGGAG
    TACAAGGCCC GGCTGGAGGA CAGCGAGGAG CGGCTGCGCC GGCAGCAGGA GGAGAAGGAC
    AGCCAGATGA AGAGCATCAT CAGCAGACTA ATGGCCGTGG AAGAGGAACT GAAGAAGGAT
    CATGCTGAGA TGCAGGCAGT TATTGATGCG AAGCAAAAAA TAATCGATGC ACAGGAAAAA
    CGGATCGTGT CGCTGGATTC GGCCAACACC AGACTGATGA GCGCGCTGAC CCAGGTGAAG
    GAACGGTACA GCATGCAGGT CCGAAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC
    ATCACGGAGA ATGGTGAATT CAAAAACAGC AGCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027831706.1
    CDS811..4689
    Translation

    Target ORF information:

    RefSeq Version XM_027975905.1
    Organism Ovis aries(sheep)
    Definition Ovis aries RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_027975905.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGAAGC AGAACCTGGT AAACACTCTG TCACTTCGGG TTCCATTCAG CAGATCTCTT 
    TCAGAGCCAG AACCCCGCTC CAGTGTCAGA TTTGTTAGAC GGTGGCCGAC TTCAGTGGGG
    TCAGTGGAGT CTGAGCAGCA GGGATATTTC ATCCGTGGCG TTTTCTCTAC TCTTTCCAAT
    GGCTTCTCCA GGATGCTGAG TCTAAAAGGA GAGTCAACCA GTGAGCCAGT AAGAGAAGAT
    GTGAAAGGGC CACCTACCCA CCGTCTGTCT TGTGGCCAGT CACCCTACAC CGACACGACC
    ACATGGGAGC GGAAGTACTG CATCCTCACA GACAGCCAGC TGGTGTTGCT CAACAAGGAG
    AAGGAGATAC CTGTGGAGGG AGGACACGAG CAGCAGGCAG ATTCCACCAA AGGGCGATGC
    CTGCGGAGAA CTGTCAGTGT TCCTTCCGAG GGTCAGTTTC CTGAATACCC ACCAGAGGGC
    GCCGCTAAAC TGGAGGTGCC AGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC
    ACTACATCTG AGAAACCCAA CTCTATGGAC ACTGCGAATA CCTCACCCTT CAAAGTACCA
    GGGTTCTTCA GCAAGCGCCT AAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT
    GACCGAAACA CGAGCTTTCG GCTTCCCTCC CTTCGCAATA CCGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAATC CCGTTCTCAT GAATCTTTGC TGAGCCCATG CAGTGCTGTG
    GAATGTCTGG ATCTTGGTAG AGGGGAGCCT GTATCAGTAA AGCCACTCCA TAGCAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGTTGT
    AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTTC GTAGGACAGT TCAACCAAAT
    AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATTATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACCAGCA AGACCAAAGC AGACAATATT TTCTGGGGTG AACATTTTGA GTTCTACAGC
    CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAGAAA
    AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGAATTAA ATCACGTTTC CAAACCATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAGT TTGCAGAATT TGTCACCAGC AACTATACCA TGTTATGTTC TGTTCTTGAA
    CCAGTAATCA GTGTGAGAAA TAAAGAAGAA CTAGCCTGTG CCTTAGTGCA CATTCTTCAA
    AGCACTGGAA GGGCCAAGGA TTTTCTGACG GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAACATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TAGTGGGACA ACAGTATCTT CACGATGCAC TGGGGGAGTT CATCAAAGCT
    TTGTATGAGT CAGATGAAAA CTGTGAAGTC GATCCCAGCA AATGTTCATC CAGTGAACTT
    CTGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTTCCTCG CGAGCTGAAA GAAGTGTTTG CATCCTGGAA GCAGCAGTGC
    CTGAACCGCG GCAAGCAAGA CATCAGCGAG CGGCTCATCA GCGCCTCGCT GTTTCTCCGT
    TTTCTGTGCC CGGCCATCAT GTCTCCCAGT CTCTTCAACC TCATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACCCT GACTCTCATT GCCAAGGTCA TCCAGAACCT GGCCAACTTT
    GCCAAATTTG GTAACAAAGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG
    TGGGAAGTAG TTTCCCAACT TGATAAGGCG ACCGTGGCAA AATTGGGACC TCTCCCTCGT
    GTTCTTGCTG ATATTACTAA ATCTCTGACT AATCCTACAC CAATACAACA GCAACTGAGA
    CGCTTCACTG AGCATAACTC CAGTCCAAAT GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG
    AAAATATTTG AAGACCCTAC TGACAGTGAT TTGCACAAAC TAAAATCCCC AAGCCAGGAC
    AACACAGACA GTTATTTCAG AGGGAAAACA TTATTGCTGG TCCAGCAAGC CTCCTCCCAG
    AGCATGACTT ACTCGGAAAA GGATGAAAAG GAAAGTAGCC TCCCCAATGG CCGCAGCATC
    TCCCTCATGG ACCTTCAGGA GTCGCACGCC GCCCAAGCGG AGCATCCGTC TGTGATGCTC
    GACGTGCCCA TGCGCTTGAC CGGAAGCCAG CTCTCCATAA CTCAGGTGGC CAGCATCAAG
    CAACTGCGGG AGACCCAGAG CACCCCCCAG AGTGCACCCC AAGTGCGAAG GCCCCTGCAC
    CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG CCCCTGTCCT TCCAGAACCC TGTCTATCAC
    CTCAGTAACC CCATTCCCGC AATGCCAAAG GCCTCTGTGG ACTCCAGTTT GGAGAACTTA
    AGCACTGCCA GTTCCAGGAG CCAAAGTAAC AGTGAAGACT TCAAACTCAG CGGACCCAGC
    AACAGCAGCA TGGAGGACTT CACCAAACGG AGCACGCAGA GTGAGGACTT CTCCAGACGC
    CACACCATGC CCGACAGACA CATACCTCTC GCCCTGCCGC GGCAGAACAG CACGGGGCAG
    ACCCAGCTCC GGAAGATGGA CCCAGCGGGG CTGGGCGCCC GAGCCAAAGC CCCGCCGTCC
    CTGCCGCACA GCGCCTCCCT GCGGAGCACC GGGAGCATGT CGGTGGCATC GGCGGCTCTG
    GTGGCCGAGC CCCTGCAGAA CGGGAGCCGG TCCCGACAGC AGTCCTCTTC CTCCAGAGAG
    AGCCCGGTCC CCAAAGTGAG AGCCATCCAG AGGCAGCAGA CCCAGCAGGT TCAGTCACCT
    GTGGACTCCG CCACAATGTC CCCAGTCGAG AGGACAGCAG CCTGGGTTCT GAACAATGGG
    CAGTATGAAG AGGACGTGGA AGAAACTGAG CAAAATCAGG ACGAAGCCAA GCATGCTGAG
    AAGTACGAAC AGGAAATCAC CAAACTGAAG GAGCGCCTGC GAGTGTCCAG CCGGCGACTC
    GAGGAGTATG AGCGCCGGTT GCTCGTGCAG GAGCAGCAGA TGCAGAGGCT GCTGCTGGAG
    TACAAGGCCC GGCTGGAGGA CAGCGAGGAG CGGCTGCGCC GGCAGCAGGA GGAGAAGGAC
    AGCCAGATGA AGAGCATCAT CAGCAGACTA ATGGCCGTGG AAGAGGAACT GAAGAAGGAT
    CATGCTGAGA TGCAGGCAGT TATTGATGCG AAGCAAAAAA TAATCGATGC ACAGGAAAAA
    CGGATCGTGT CGCTGGATTC GGCCAACACC AGACTGATGA GCGCGCTGAC CCAGGTGAAG
    GAACGGTACA GCATGCAGGT CCGAAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC
    ATCACGGAGA ATGGTGAATT CAAAAACAGC AGCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm61884
    Clone ID Related Accession (Same CDS sequence) XM_027975906.1
    Accession Version XM_027975906.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3873bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-05
    Organism Ovis aries(sheep)
    Product ras GTPase-activating protein nGAP isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCACCCT ACACCGACAC GACCACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA CGAGCAGCAG
    GCAGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCGCT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAATCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCCCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AATCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC TGTGGAATGT CTGGATCTTG GTAGAGGGGA GCCTGTATCA
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGAA TTAAATCACG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAACTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTAGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCTTTGTAT GAGTCAGATG AAAACTGTGA AGTCGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGCGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AAGACATCAG CGAGCGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CCCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AAGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT
    TCCTTCCTAC AGGCGACCGT GGCAAAATTG GGACCTCTCC CTCGTGTTCT TGCTGATATT
    ACTAAATCTC TGACTAATCC TACACCAATA CAACAGCAAC TGAGACGCTT CACTGAGCAT
    AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC
    CCTACTGACA GTGATTTGCA CAAACTAAAA TCCCCAAGCC AGGACAACAC AGACAGTTAT
    TTCAGAGGGA AAACATTATT GCTGGTCCAG CAAGCCTCCT CCCAGAGCAT GACTTACTCG
    GAAAAGGATG AAAAGGAAAG TAGCCTCCCC AATGGCCGCA GCATCTCCCT CATGGACCTT
    CAGGAGTCGC ACGCCGCCCA AGCGGAGCAT CCGTCTGTGA TGCTCGACGT GCCCATGCGC
    TTGACCGGAA GCCAGCTCTC CATAACTCAG GTGGCCAGCA TCAAGCAACT GCGGGAGACC
    CAGAGCACCC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCC TGCACCCAGC CTTGAACCAG
    CCAGGCAGCC TCCAGCCCCT GTCCTTCCAG AACCCTGTCT ATCACCTCAG TAACCCCATT
    CCCGCAATGC CAAAGGCCTC TGTGGACTCC AGTTTGGAGA ACTTAAGCAC TGCCAGTTCC
    AGGAGCCAAA GTAACAGTGA AGACTTCAAA CTCAGCGGAC CCAGCAACAG CAGCATGGAG
    GACTTCACCA AACGGAGCAC GCAGAGTGAG GACTTCTCCA GACGCCACAC CATGCCCGAC
    AGACACATAC CTCTCGCCCT GCCGCGGCAG AACAGCACGG GGCAGACCCA GCTCCGGAAG
    ATGGACCCAG CGGGGCTGGG CGCCCGAGCC AAAGCCCCGC CGTCCCTGCC GCACAGCGCC
    TCCCTGCGGA GCACCGGGAG CATGTCGGTG GCATCGGCGG CTCTGGTGGC CGAGCCCCTG
    CAGAACGGGA GCCGGTCCCG ACAGCAGTCC TCTTCCTCCA GAGAGAGCCC GGTCCCCAAA
    GTGAGAGCCA TCCAGAGGCA GCAGACCCAG CAGGTTCAGT CACCTGTGGA CTCCGCCACA
    ATGTCCCCAG TCGAGAGGAC AGCAGCCTGG GTTCTGAACA ATGGGCAGTA TGAAGAGGAC
    GTGGAAGAAA CTGAGCAAAA TCAGGACGAA GCCAAGCATG CTGAGAAGTA CGAACAGGAA
    ATCACCAAAC TGAAGGAGCG CCTGCGAGTG TCCAGCCGGC GACTCGAGGA GTATGAGCGC
    CGGTTGCTCG TGCAGGAGCA GCAGATGCAG AGGCTGCTGC TGGAGTACAA GGCCCGGCTG
    GAGGACAGCG AGGAGCGGCT GCGCCGGCAG CAGGAGGAGA AGGACAGCCA GATGAAGAGC
    ATCATCAGCA GACTAATGGC CGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAG
    GCAGTTATTG ATGCGAAGCA AAAAATAATC GATGCACAGG TCATAAATGG AGATGAGATT
    ATGCAAACAG GAAAAACGGA TCGTGTCGCT GGATTCGGCC AACACCAGAC TGATGAGCGC
    GCTGACCCAG GTGAAGGAAC GGTACAGCAT GCAGGTCCGA AATGGCATCT CCCCCACCAA
    CCCCACCAAG CTTTCCATCA CGGAGAATGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027831707.1
    CDS64..3936
    Translation

    Target ORF information:

    RefSeq Version XM_027975906.1
    Organism Ovis aries(sheep)
    Definition Ovis aries RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_027975906.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCACCCT ACACCGACAC GACCACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA CGAGCAGCAG
    GCAGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCGCT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAATCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCCCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AATCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC TGTGGAATGT CTGGATCTTG GTAGAGGGGA GCCTGTATCA
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGAA TTAAATCACG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAACTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTAGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCTTTGTAT GAGTCAGATG AAAACTGTGA AGTCGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGCGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AAGACATCAG CGAGCGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CCCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AAGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT
    TCCTTCCTAC AGGCGACCGT GGCAAAATTG GGACCTCTCC CTCGTGTTCT TGCTGATATT
    ACTAAATCTC TGACTAATCC TACACCAATA CAACAGCAAC TGAGACGCTT CACTGAGCAT
    AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC
    CCTACTGACA GTGATTTGCA CAAACTAAAA TCCCCAAGCC AGGACAACAC AGACAGTTAT
    TTCAGAGGGA AAACATTATT GCTGGTCCAG CAAGCCTCCT CCCAGAGCAT GACTTACTCG
    GAAAAGGATG AAAAGGAAAG TAGCCTCCCC AATGGCCGCA GCATCTCCCT CATGGACCTT
    CAGGAGTCGC ACGCCGCCCA AGCGGAGCAT CCGTCTGTGA TGCTCGACGT GCCCATGCGC
    TTGACCGGAA GCCAGCTCTC CATAACTCAG GTGGCCAGCA TCAAGCAACT GCGGGAGACC
    CAGAGCACCC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCC TGCACCCAGC CTTGAACCAG
    CCAGGCAGCC TCCAGCCCCT GTCCTTCCAG AACCCTGTCT ATCACCTCAG TAACCCCATT
    CCCGCAATGC CAAAGGCCTC TGTGGACTCC AGTTTGGAGA ACTTAAGCAC TGCCAGTTCC
    AGGAGCCAAA GTAACAGTGA AGACTTCAAA CTCAGCGGAC CCAGCAACAG CAGCATGGAG
    GACTTCACCA AACGGAGCAC GCAGAGTGAG GACTTCTCCA GACGCCACAC CATGCCCGAC
    AGACACATAC CTCTCGCCCT GCCGCGGCAG AACAGCACGG GGCAGACCCA GCTCCGGAAG
    ATGGACCCAG CGGGGCTGGG CGCCCGAGCC AAAGCCCCGC CGTCCCTGCC GCACAGCGCC
    TCCCTGCGGA GCACCGGGAG CATGTCGGTG GCATCGGCGG CTCTGGTGGC CGAGCCCCTG
    CAGAACGGGA GCCGGTCCCG ACAGCAGTCC TCTTCCTCCA GAGAGAGCCC GGTCCCCAAA
    GTGAGAGCCA TCCAGAGGCA GCAGACCCAG CAGGTTCAGT CACCTGTGGA CTCCGCCACA
    ATGTCCCCAG TCGAGAGGAC AGCAGCCTGG GTTCTGAACA ATGGGCAGTA TGAAGAGGAC
    GTGGAAGAAA CTGAGCAAAA TCAGGACGAA GCCAAGCATG CTGAGAAGTA CGAACAGGAA
    ATCACCAAAC TGAAGGAGCG CCTGCGAGTG TCCAGCCGGC GACTCGAGGA GTATGAGCGC
    CGGTTGCTCG TGCAGGAGCA GCAGATGCAG AGGCTGCTGC TGGAGTACAA GGCCCGGCTG
    GAGGACAGCG AGGAGCGGCT GCGCCGGCAG CAGGAGGAGA AGGACAGCCA GATGAAGAGC
    ATCATCAGCA GACTAATGGC CGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAG
    GCAGTTATTG ATGCGAAGCA AAAAATAATC GATGCACAGG TCATAAATGG AGATGAGATT
    ATGCAAACAG GAAAAACGGA TCGTGTCGCT GGATTCGGCC AACACCAGAC TGATGAGCGC
    GCTGACCCAG GTGAAGGAAC GGTACAGCAT GCAGGTCCGA AATGGCATCT CCCCCACCAA
    CCCCACCAAG CTTTCCATCA CGGAGAATGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm61885
    Clone ID Related Accession (Same CDS sequence) XM_027975907.1
    Accession Version XM_027975907.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3864bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-05
    Organism Ovis aries(sheep)
    Product ras GTPase-activating protein nGAP isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCACCCT ACACCGACAC GACCACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA CGAGCAGCAG
    GCAGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCGCT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAATCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCCCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AATCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC TGTGGAATGT CTGGATCTTG GTAGAGGGGA GCCTGTATCA
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGAA TTAAATCACG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAACTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTAGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCTTTGTAT GAGTCAGATG AAAACTGTGA AGTCGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGCGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AAGACATCAG CGAGCGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CCCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AAGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT
    TCCTTCCTAC AGGCGACCGT GGCAAAATTG GGACCTCTCC CTCGTGTTCT TGCTGATATT
    ACTAAATCTC TGACTAATCC TACACCAATA CAACAGCAAC TGAGACGCTT CACTGAGCAT
    AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC
    CCTACTGACA GTGATTTGCA CAAACTAAAA TCCCCAAGCC AGGACAACAC AGACAGTTAT
    TTCAGAGGGA AAACATTATT GCTGGTCCAG CAAGCCTCCT CCCAGAGCAT GACTTACTCG
    GAAAAGGATG AAAAGGAAAG TAGCCTCCCC AATGGCCGCA GCATCTCCCT CATGGACCTT
    CAGGAGTCGC ACGCCGCCCA AGCGGAGCAT CCGTCTGTGA TGCTCGACGT GCCCATGCGC
    TTGACCGGAA GCCAGCTCTC CATAACTCAG GTGGCCAGCA TCAAGCAACT GCGGGAGACC
    CAGAGCACCC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCC TGCACCCAGC CTTGAACCAG
    CCAGGCAGCC TCCAGCCCCT GTCCTTCCAG AACCCTGTCT ATCACCTCAG TAACCCCATT
    CCCGCAATGC CAAAGGCCTC TGTGGACTCC AGTTTGGAGA ACTTAAGCAC TGCCAGTTCC
    AGGAGCCAAA GTAACAGTGA AGACTTCAAA CTCAGCGGAC CCAGCAACAG CAGCATGGAG
    GACTTCACCA AACGGAGCAC GCAGAGTGAG GACTTCTCCA GACGCCACAC CATGCCCGAC
    AGACACATAC CTCTCGCCCT GCCGCGGCAG AACAGCACGG GGCAGACCCA GCTCCGGAAG
    ATGGACCCAG CGGGGCTGGG CGCCCGAGCC AAAGCCCCGC CGTCCCTGCC GCACAGCGCC
    TCCCTGCGGA GCACCGGGAG CATGTCGGTG GCATCGGCGG CTCTGGTGGC CGAGCCCCTG
    CAGAACGGGA GCCGGTCCCG ACAGCAGTCC TCTTCCTCCA GAGAGAGCCC GGTCCCCAAA
    GTGAGAGCCA TCCAGAGGCA GCAGACCCAG CAGGTTCAGT CACCTGTGGA CTCCGCCACA
    ATGTCCCCAG TCGAGAGGAC AGCAGCCTGG GTTCTGAACA ATGGGCAGTA TGAAGAGGAC
    GTGGAAGAAA CTGAGCAAAA TCAGGACGAA GCCAAGCATG CTGAGAAGTA CGAACAGGAA
    ATCACCAAAC TGAAGGAGCG CCTGCGAGTG TCCAGCCGGC GACTCGAGGA GTATGAGCGC
    CGGTTGCTCG TGCAGGAGCA GCAGATGCAG AGGCTGCTGC TGGAGTACAA GGCCCGGCTG
    GAGGACAGCG AGGAGCGGCT GCGCCGGCAG CAGGAGGAGA AGGACAGCCA GATGAAGAGC
    ATCATCAGCA GACTAATGGC CGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAG
    GCAGTTATTG ATGCGAAGCA AAAAATAATC GATGCACAGG AAAAACGGAT CGTGTCGCTG
    GATTCGGCCA ACACCAGACT GATGAGCGCG CTGACCCAGG TGAAGGAACG GTACAGCATG
    CAGGTCCGAA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC GGAGAATGGT
    GAATTCAAAA ACAGCAGCTG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027831708.1
    CDS63..3926
    Translation

    Target ORF information:

    RefSeq Version XM_027975907.1
    Organism Ovis aries(sheep)
    Definition Ovis aries RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_027975907.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCACCCT ACACCGACAC GACCACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA CGAGCAGCAG
    GCAGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCGCT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAATCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCCCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AATCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC TGTGGAATGT CTGGATCTTG GTAGAGGGGA GCCTGTATCA
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGAA TTAAATCACG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAACTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTAGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCTTTGTAT GAGTCAGATG AAAACTGTGA AGTCGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGCGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AAGACATCAG CGAGCGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CCCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AAGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT
    TCCTTCCTAC AGGCGACCGT GGCAAAATTG GGACCTCTCC CTCGTGTTCT TGCTGATATT
    ACTAAATCTC TGACTAATCC TACACCAATA CAACAGCAAC TGAGACGCTT CACTGAGCAT
    AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC
    CCTACTGACA GTGATTTGCA CAAACTAAAA TCCCCAAGCC AGGACAACAC AGACAGTTAT
    TTCAGAGGGA AAACATTATT GCTGGTCCAG CAAGCCTCCT CCCAGAGCAT GACTTACTCG
    GAAAAGGATG AAAAGGAAAG TAGCCTCCCC AATGGCCGCA GCATCTCCCT CATGGACCTT
    CAGGAGTCGC ACGCCGCCCA AGCGGAGCAT CCGTCTGTGA TGCTCGACGT GCCCATGCGC
    TTGACCGGAA GCCAGCTCTC CATAACTCAG GTGGCCAGCA TCAAGCAACT GCGGGAGACC
    CAGAGCACCC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCC TGCACCCAGC CTTGAACCAG
    CCAGGCAGCC TCCAGCCCCT GTCCTTCCAG AACCCTGTCT ATCACCTCAG TAACCCCATT
    CCCGCAATGC CAAAGGCCTC TGTGGACTCC AGTTTGGAGA ACTTAAGCAC TGCCAGTTCC
    AGGAGCCAAA GTAACAGTGA AGACTTCAAA CTCAGCGGAC CCAGCAACAG CAGCATGGAG
    GACTTCACCA AACGGAGCAC GCAGAGTGAG GACTTCTCCA GACGCCACAC CATGCCCGAC
    AGACACATAC CTCTCGCCCT GCCGCGGCAG AACAGCACGG GGCAGACCCA GCTCCGGAAG
    ATGGACCCAG CGGGGCTGGG CGCCCGAGCC AAAGCCCCGC CGTCCCTGCC GCACAGCGCC
    TCCCTGCGGA GCACCGGGAG CATGTCGGTG GCATCGGCGG CTCTGGTGGC CGAGCCCCTG
    CAGAACGGGA GCCGGTCCCG ACAGCAGTCC TCTTCCTCCA GAGAGAGCCC GGTCCCCAAA
    GTGAGAGCCA TCCAGAGGCA GCAGACCCAG CAGGTTCAGT CACCTGTGGA CTCCGCCACA
    ATGTCCCCAG TCGAGAGGAC AGCAGCCTGG GTTCTGAACA ATGGGCAGTA TGAAGAGGAC
    GTGGAAGAAA CTGAGCAAAA TCAGGACGAA GCCAAGCATG CTGAGAAGTA CGAACAGGAA
    ATCACCAAAC TGAAGGAGCG CCTGCGAGTG TCCAGCCGGC GACTCGAGGA GTATGAGCGC
    CGGTTGCTCG TGCAGGAGCA GCAGATGCAG AGGCTGCTGC TGGAGTACAA GGCCCGGCTG
    GAGGACAGCG AGGAGCGGCT GCGCCGGCAG CAGGAGGAGA AGGACAGCCA GATGAAGAGC
    ATCATCAGCA GACTAATGGC CGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAG
    GCAGTTATTG ATGCGAAGCA AAAAATAATC GATGCACAGG AAAAACGGAT CGTGTCGCTG
    GATTCGGCCA ACACCAGACT GATGAGCGCG CTGACCCAGG TGAAGGAACG GTACAGCATG
    CAGGTCCGAA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC GGAGAATGGT
    GAATTCAAAA ACAGCAGCTG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm61886
    Clone ID Related Accession (Same CDS sequence) XM_015099332.2
    Accession Version XM_015099332.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-05
    Organism Ovis aries(sheep)
    Product ras GTPase-activating protein nGAP isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_015099332.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCACCCT ACACCGACAC GACCACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA CGAGCAGCAG
    GCAGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCGCT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAATCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCCCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AATCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC TGTGGAATGT CTGGATCTTG GTAGAGGGGA GCCTGTATCA
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGAA TTAAATCACG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAACTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTAGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCTTTGTAT GAGTCAGATG AAAACTGTGA AGTCGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGCGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AAGACATCAG CGAGCGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CCCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AAGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGCGACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CTAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA AACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGTCGCA CGCCGCCCAA
    GCGGAGCATC CGTCTGTGAT GCTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CCGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CTTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG ACGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCAGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCGGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TTCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAAAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGAGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTCGT GCAGGAGCAG
    CAGATGCAGA GGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCCGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG ACTAATGGCC
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCGAAGCAA
    AAAATAATCG ATGCACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA CACCAGACTG
    ATGAGCGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA TGGCATCTCC
    CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014954818.2
    CDS63..3905
    Translation

    Target ORF information:

    RefSeq Version XM_015099332.2
    Organism Ovis aries(sheep)
    Definition Ovis aries RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015099332.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
    CCGGGATTGG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC
    AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC
    CAGTCACCCT ACACCGACAC GACCACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC
    CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA CGAGCAGCAG
    GCAGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG
    TTTCCTGAAT ACCCACCAGA GGGCGCCGCT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA
    CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG
    AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG
    AGGACCAAAA GCCAATCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC CTCCCTTCGC
    AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AATCCCGTTC TCATGAATCT
    TTGCTGAGCC CATGCAGTGC TGTGGAATGT CTGGATCTTG GTAGAGGGGA GCCTGTATCA
    GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC
    CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT
    TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT
    AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGAA TTAAATCACG TTTCCAAACC
    ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT
    ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAACTAGCC
    TGTGCCTTAG TGCACATTCT TCAAAGCACT GGAAGGGCCA AGGATTTTCT GACGGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTAGTGG GACAACAGTA TCTTCACGAT
    GCACTGGGGG AGTTCATCAA AGCTTTGTAT GAGTCAGATG AAAACTGTGA AGTCGATCCC
    AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGCGAGCT GAAAGAAGTG
    TTTGCATCCT GGAAGCAGCA GTGCCTGAAC CGCGGCAAGC AAGACATCAG CGAGCGGCTC
    ATCAGCGCCT CGCTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC
    AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CCCTGACTCT CATTGCCAAG
    GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AAGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGCGACCGTG
    GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CTAAATCTCT GACTAATCCT
    ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC
    AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA AACATTATTG
    CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT
    AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGTCGCA CGCCGCCCAA
    GCGGAGCATC CGTCTGTGAT GCTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC
    ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA
    CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCCTG
    TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CCGCAATGCC AAAGGCCTCT
    GTGGACTCCA GTTTGGAGAA CTTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA
    GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG
    CAGAGTGAGG ACTTCTCCAG ACGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG
    CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCAGC GGGGCTGGGC
    GCCCGAGCCA AAGCCCCGCC GTCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC
    ATGTCGGTGG CATCGGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTCCCCAAAG TGAGAGCCAT CCAGAGGCAG
    CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA
    GCAGCCTGGG TTCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAAAC TGAGCAAAAT
    CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC
    CTGCGAGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTCGT GCAGGAGCAG
    CAGATGCAGA GGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG
    CGCCGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG ACTAATGGCC
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCGAAGCAA
    AAAATAATCG ATGCACAGGA AAAACGGATC GTGTCGCTGG ATTCGGCCAA CACCAGACTG
    ATGAGCGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA TGGCATCTCC
    CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm61887
    Clone ID Related Accession (Same CDS sequence) XM_027975908.1
    Accession Version XM_027975908.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-05
    Organism Ovis aries(sheep)
    Product ras GTPase-activating protein nGAP isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGACCC CAGAGGTGCC AGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
    ACTACATCTG AGAAACCCAA CTCTATGGAC ACTGCGAATA CCTCACCCTT CAAAGTACCA
    GGGTTCTTCA GCAAGCGCCT AAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT
    GACCGAAACA CGAGCTTTCG GCTTCCCTCC CTTCGCAATA CCGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAATC CCGTTCTCAT GAATCTTTGC TGAGCCCATG CAGTGCTGTG
    GAATGTCTGG ATCTTGGTAG AGGGGAGCCT GTATCAGTAA AGCCACTCCA TAGCAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGTTGT
    AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTTC GTAGGACAGT TCAACCAAAT
    AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATTATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACCAGCA AGACCAAAGC AGACAATATT TTCTGGGGTG AACATTTTGA GTTCTACAGC
    CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAGAAA
    AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGAATTAA ATCACGTTTC CAAACCATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAGT TTGCAGAATT TGTCACCAGC AACTATACCA TGTTATGTTC TGTTCTTGAA
    CCAGTAATCA GTGTGAGAAA TAAAGAAGAA CTAGCCTGTG CCTTAGTGCA CATTCTTCAA
    AGCACTGGAA GGGCCAAGGA TTTTCTGACG GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAACATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TAGTGGGACA ACAGTATCTT CACGATGCAC TGGGGGAGTT CATCAAAGCT
    TTGTATGAGT CAGATGAAAA CTGTGAAGTC GATCCCAGCA AATGTTCATC CAGTGAACTT
    CTGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTTCCTCG CGAGCTGAAA GAAGTGTTTG CATCCTGGAA GCAGCAGTGC
    CTGAACCGCG GCAAGCAAGA CATCAGCGAG CGGCTCATCA GCGCCTCGCT GTTTCTCCGT
    TTTCTGTGCC CGGCCATCAT GTCTCCCAGT CTCTTCAACC TCATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACCCT GACTCTCATT GCCAAGGTCA TCCAGAACCT GGCCAACTTT
    GCCAAATTTG GTAACAAAGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA
    AAATTGGGAC CTCTCCCTCG TGTTCTTGCT GATATTACTA AATCTCTGAC TAATCCTACA
    CCAATACAAC AGCAACTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA
    AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCTA CTGACAGTGA TTTGCACAAA
    CTAAAATCCC CAAGCCAGGA CAACACAGAC AGTTATTTCA GAGGGAAAAC ATTATTGCTG
    GTCCAGCAAG CCTCCTCCCA GAGCATGACT TACTCGGAAA AGGATGAAAA GGAAAGTAGC
    CTCCCCAATG GCCGCAGCAT CTCCCTCATG GACCTTCAGG AGTCGCACGC CGCCCAAGCG
    GAGCATCCGT CTGTGATGCT CGACGTGCCC ATGCGCTTGA CCGGAAGCCA GCTCTCCATA
    ACTCAGGTGG CCAGCATCAA GCAACTGCGG GAGACCCAGA GCACCCCCCA GAGTGCACCC
    CAAGTGCGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCC
    TTCCAGAACC CTGTCTATCA CCTCAGTAAC CCCATTCCCG CAATGCCAAA GGCCTCTGTG
    GACTCCAGTT TGGAGAACTT AAGCACTGCC AGTTCCAGGA GCCAAAGTAA CAGTGAAGAC
    TTCAAACTCA GCGGACCCAG CAACAGCAGC ATGGAGGACT TCACCAAACG GAGCACGCAG
    AGTGAGGACT TCTCCAGACG CCACACCATG CCCGACAGAC ACATACCTCT CGCCCTGCCG
    CGGCAGAACA GCACGGGGCA GACCCAGCTC CGGAAGATGG ACCCAGCGGG GCTGGGCGCC
    CGAGCCAAAG CCCCGCCGTC CCTGCCGCAC AGCGCCTCCC TGCGGAGCAC CGGGAGCATG
    TCGGTGGCAT CGGCGGCTCT GGTGGCCGAG CCCCTGCAGA ACGGGAGCCG GTCCCGACAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCGGTC CCCAAAGTGA GAGCCATCCA GAGGCAGCAG
    ACCCAGCAGG TTCAGTCACC TGTGGACTCC GCCACAATGT CCCCAGTCGA GAGGACAGCA
    GCCTGGGTTC TGAACAATGG GCAGTATGAA GAGGACGTGG AAGAAACTGA GCAAAATCAG
    GACGAAGCCA AGCATGCTGA GAAGTACGAA CAGGAAATCA CCAAACTGAA GGAGCGCCTG
    CGAGTGTCCA GCCGGCGACT CGAGGAGTAT GAGCGCCGGT TGCTCGTGCA GGAGCAGCAG
    ATGCAGAGGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ACAGCGAGGA GCGGCTGCGC
    CGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAGAGCATCA TCAGCAGACT AATGGCCGTG
    GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAGGCAG TTATTGATGC GAAGCAAAAA
    ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATGC AAACAGGAAA AACGGATCGT
    GTCGCTGGAT TCGGCCAACA CCAGACTGAT GAGCGCGCTG ACCCAGGTGA AGGAACGGTA
    CAGCATGCAG GTCCGAAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027831709.1
    CDS740..4168
    Translation

    Target ORF information:

    RefSeq Version XM_027975908.1
    Organism Ovis aries(sheep)
    Definition Ovis aries RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_027975908.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGACCC CAGAGGTGCC AGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
    ACTACATCTG AGAAACCCAA CTCTATGGAC ACTGCGAATA CCTCACCCTT CAAAGTACCA
    GGGTTCTTCA GCAAGCGCCT AAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT
    GACCGAAACA CGAGCTTTCG GCTTCCCTCC CTTCGCAATA CCGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAATC CCGTTCTCAT GAATCTTTGC TGAGCCCATG CAGTGCTGTG
    GAATGTCTGG ATCTTGGTAG AGGGGAGCCT GTATCAGTAA AGCCACTCCA TAGCAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGTTGT
    AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTTC GTAGGACAGT TCAACCAAAT
    AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATTATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACCAGCA AGACCAAAGC AGACAATATT TTCTGGGGTG AACATTTTGA GTTCTACAGC
    CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAGAAA
    AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGAATTAA ATCACGTTTC CAAACCATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAGT TTGCAGAATT TGTCACCAGC AACTATACCA TGTTATGTTC TGTTCTTGAA
    CCAGTAATCA GTGTGAGAAA TAAAGAAGAA CTAGCCTGTG CCTTAGTGCA CATTCTTCAA
    AGCACTGGAA GGGCCAAGGA TTTTCTGACG GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAACATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TAGTGGGACA ACAGTATCTT CACGATGCAC TGGGGGAGTT CATCAAAGCT
    TTGTATGAGT CAGATGAAAA CTGTGAAGTC GATCCCAGCA AATGTTCATC CAGTGAACTT
    CTGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTTCCTCG CGAGCTGAAA GAAGTGTTTG CATCCTGGAA GCAGCAGTGC
    CTGAACCGCG GCAAGCAAGA CATCAGCGAG CGGCTCATCA GCGCCTCGCT GTTTCTCCGT
    TTTCTGTGCC CGGCCATCAT GTCTCCCAGT CTCTTCAACC TCATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACCCT GACTCTCATT GCCAAGGTCA TCCAGAACCT GGCCAACTTT
    GCCAAATTTG GTAACAAAGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA
    AAATTGGGAC CTCTCCCTCG TGTTCTTGCT GATATTACTA AATCTCTGAC TAATCCTACA
    CCAATACAAC AGCAACTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA
    AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCTA CTGACAGTGA TTTGCACAAA
    CTAAAATCCC CAAGCCAGGA CAACACAGAC AGTTATTTCA GAGGGAAAAC ATTATTGCTG
    GTCCAGCAAG CCTCCTCCCA GAGCATGACT TACTCGGAAA AGGATGAAAA GGAAAGTAGC
    CTCCCCAATG GCCGCAGCAT CTCCCTCATG GACCTTCAGG AGTCGCACGC CGCCCAAGCG
    GAGCATCCGT CTGTGATGCT CGACGTGCCC ATGCGCTTGA CCGGAAGCCA GCTCTCCATA
    ACTCAGGTGG CCAGCATCAA GCAACTGCGG GAGACCCAGA GCACCCCCCA GAGTGCACCC
    CAAGTGCGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCCTGTCC
    TTCCAGAACC CTGTCTATCA CCTCAGTAAC CCCATTCCCG CAATGCCAAA GGCCTCTGTG
    GACTCCAGTT TGGAGAACTT AAGCACTGCC AGTTCCAGGA GCCAAAGTAA CAGTGAAGAC
    TTCAAACTCA GCGGACCCAG CAACAGCAGC ATGGAGGACT TCACCAAACG GAGCACGCAG
    AGTGAGGACT TCTCCAGACG CCACACCATG CCCGACAGAC ACATACCTCT CGCCCTGCCG
    CGGCAGAACA GCACGGGGCA GACCCAGCTC CGGAAGATGG ACCCAGCGGG GCTGGGCGCC
    CGAGCCAAAG CCCCGCCGTC CCTGCCGCAC AGCGCCTCCC TGCGGAGCAC CGGGAGCATG
    TCGGTGGCAT CGGCGGCTCT GGTGGCCGAG CCCCTGCAGA ACGGGAGCCG GTCCCGACAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCGGTC CCCAAAGTGA GAGCCATCCA GAGGCAGCAG
    ACCCAGCAGG TTCAGTCACC TGTGGACTCC GCCACAATGT CCCCAGTCGA GAGGACAGCA
    GCCTGGGTTC TGAACAATGG GCAGTATGAA GAGGACGTGG AAGAAACTGA GCAAAATCAG
    GACGAAGCCA AGCATGCTGA GAAGTACGAA CAGGAAATCA CCAAACTGAA GGAGCGCCTG
    CGAGTGTCCA GCCGGCGACT CGAGGAGTAT GAGCGCCGGT TGCTCGTGCA GGAGCAGCAG
    ATGCAGAGGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ACAGCGAGGA GCGGCTGCGC
    CGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAGAGCATCA TCAGCAGACT AATGGCCGTG
    GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAGGCAG TTATTGATGC GAAGCAAAAA
    ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATGC AAACAGGAAA AACGGATCGT
    GTCGCTGGAT TCGGCCAACA CCAGACTGAT GAGCGCGCTG ACCCAGGTGA AGGAACGGTA
    CAGCATGCAG GTCCGAAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.