LRRK1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005221724.3	XP_005221781.1	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
XM_015459145.1	XP_015314631.1	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
XM_010816886.2	XP_010815188.1	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
XM_005221725.3	XP_005221782.1	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
XM_024981744.1	XP_024837512.1	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
XM_005221726.4	XP_005221783.1	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
XM_010816887.3	XP_010815189.1	leucine-rich repeat serine/threonine-protein kinase 1 isoform X2
NM_001205774.2	NP_001192703.1	leucine-rich repeat serine/threonine-protein kinase 1
NM_001205774.1	NP_001192703.1	leucine-rich repeat serine/threonine-protein kinase 1
XM_010816887.2	XP_010815189.1	leucine-rich repeat serine/threonine-protein kinase 1 isoform X2
XM_005221726.3	XP_005221783.1	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
XM_024981743.1	XP_024837511.1	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
XM_005221725.4	XP_005221782.1	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
XM_005221724.4	XP_005221781.1	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa48783	XM_010816887.3 Latest version!	Bos taurus leucine rich repeat kinase 1 (LRRK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa30787	NM_001205774.2 Latest version!	Bos taurus leucine rich repeat kinase 1 (LRRK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa30787	XM_010816886.2 Latest version!	Bos taurus leucine-rich repeat kinase 1 (LRRK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa30787	XM_024981744.1 Latest version!	Bos taurus leucine rich repeat kinase 1 (LRRK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa30787	XM_005221725.4 Latest version!	Bos taurus leucine rich repeat kinase 1 (LRRK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa30787	XM_005221726.4 Latest version!	Bos taurus leucine rich repeat kinase 1 (LRRK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa30787	XM_005221724.4 Latest version!	Bos taurus leucine rich repeat kinase 1 (LRRK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa30787	XM_024981743.1 Latest version!	Bos taurus leucine rich repeat kinase 1 (LRRK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa30787	XM_015459145.1 Latest version!	Bos taurus leucine-rich repeat kinase 1 (LRRK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OBa30787	NM_001205774.1	Bos taurus leucine-rich repeat kinase 1 (LRRK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa48783	XM_010816887.2	Bos taurus leucine-rich repeat kinase 1 (LRRK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa30787	XM_005221726.3	Bos taurus leucine-rich repeat kinase 1 (LRRK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa30787	XM_005221725.3	Bos taurus leucine-rich repeat kinase 1 (LRRK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa30787	XM_005221724.3	Bos taurus leucine-rich repeat kinase 1 (LRRK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

LRRK1 ( XM_010816887.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815189.1 Bos taurus leucine rich repeat kinase 1 (LRRK1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa48783
Clone ID Related Accession (Same CDS sequence)	XM_010816887.3 , XM_010816887.2
Accession Version	XM_010816887.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	leucine-rich repeat serine/threonine-protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816887.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACTC GGGAGCCAGA GGCTCTGCGT GTGAAGTGGT CCCACCTCAA GCTGCCCTGG 
GTGGATCTGG ACTGGCTCAT CGACATCTCC TGCCAGATCA CAGAGCTGGA TCTTTCTGCC 
AACTGCCTGG CGTCCCTCCC CTCAGTCGTC CCCTGGGGCC TCATCAACCT CCGGAAGCTG 
AACCTCTCAG ACAACCACCT GGGGGAGCTG CCCAGCGTGC AGTCATCAGA TGAAATCATC 
TGTTCCAGGC TACTTGAAAT TGACATTTCC AGCAACAAGT TGTCCCACCT CCCACCAGGA 
TTCCTGCATC TCTCAAAACT TCAAAAACTG ACGGCTTCAA AAAACTATTT GGAAAAATTG 
TTTGAAGAAG AGAGTGCCAC GAACTGGATT GGTTTACGGA AGCTACAGGA GCTCGATGTT 
TCTGACAACA AACTGACAGA ACTCCCTGCA CTTTTCCTCC ATTCTTTCAA ATCGCTCAGT 
TGTCTAAACG TCTCCAGAAA TAACCTGAAG GCGTTTCCAG ATGCCTGGGC TTGCCCTTTG 
AAATGTTGCA AAGCATCCAA AAATGCCCTG GAGTCTCTAC CAGACAAAAT GGCTGTGTTT 
TGGAAGAACC ACTTGAAGGA TGTGGACTTC TCAGAAAACG CACTGAAAGA AGTTCCCCTG 
GGACTTTTCC AGCTTGATGC CCTCATGTTC TTGAAGTTAC AGGGAAACCA GCTGGTGGCA 
CTCCCACCTC AAGAGAAGTG GACCTGCAGA CAGCTCAAAA CCCTGGATCT CTCCAGAAAT 
CACTTTGGCA AAAATGAAGA CGTCCTTAAA ACAAAGCGCA TTTCCTTTTT CACCACCAGA 
GGCCGCCAGC GTTCGGGGAC GGAGGCAGCG TGTGTACTTG AATTTCCCGC CTTCCTAAGT 
GAGTCTTTGG AAGTCCTCTG TCTGAACGAC AACCACCTGG ATGCAGTCCC GCCCTCAGTT 
TGCCTACTGA AGAGCTTATC AGAACTCTAC TTGGGGAACA ATCCTAGCCT CCGAGAGCTC 
CCTCCTGAGC TGGGGCAGCT GGGGAACCTC TGGCAGTTGG ACATTGAAGA CCTGAATATC 
ATCAACGTGC CTGCAGAGAT CAGAAAAGAA GGCCCCAAAG CAATGCTGTC TTACCTGCGT 
GCTCAGCTGC GGAAAGCAGA GAAGTGCAAG CAGATGAAGA TGATCATCGT GGGTCCGCCG 
CGTCAGGGCA AGTCCACCCT CCTGGAGATC TTACAGACGG GGAGGGCCCC CCAGGTGGTA 
CACAGCGAGG CCACCATCAG GACCACCAAG TGGGAGCTGC AGAGGCCGGC TGGCTCGAGA 
GCCAAGGTTG AGTCTGTGGA GTTCACCGTC TGGGACATCG GCGGCCCCGC CAGCATGGCC 
ACGGTCAACC AGTGCTTCTT CACAGACAAG GCCCTGTACG TGGTGGTCTG GAACCTGGCT 
CTGGGGGAGG AGGCCGTGGC CAGCCTTCAG TTCTGGCTGC TCAACATCGA GGCCAAGGCC 
CCAAATGCTG TGGTGCTGGT GGTGGGAACA CATCTGGACT TAATTGAAAC CAAATTCCGC 
GTGGAGAGGA TCGCCACTCT GCGCGCCTAC GTGCTGGCCC TCTGCCGCTC GCCCTCTGGG 
TCCAGGGCCA CCGGCTTCCC GGACATCACG TGTAAACACC TGCACGAACT CTCCTGTAAG 
AGCTTGGAAG GTCAGGAAGG GCTGCGGCAG CTCATTTTCC AGGTCACGTG CAACATGAAG 
GACGTGGGCA GCACCATCGG CTGTCAGCGA CTTGCAGGGA GGCTGATCCC CAGGAGCTAC 
TTGAGCCTCC AGGAGGCTGT GCTAGCAGAG AAGCAGCACC GCAGCCTGAG TGACGATGTG 
CAGTACCTGA CAGACCGGCA GCTAGAGCAG CTGGTGGACC AGATGCCCTG CAGCGACATC 
CAGGACTACG AGGACCTGCA GTCTGCCATC AGCTTCCTCA TTGAAACGGG CTCCCTGCTG 
CACTTCCCGG ACACCAGCCA CGGCCTGAGG AACCTCTACT TCCTCGACCC CATCTGGCTG 
TCCGAGTGTC TGCAGAGGAT CTTCAACATC AAGGGCTCTC GGTCAGTGGC CAAGAATGGG 
GTGATCAGAG CCGAGGACCT TCGGCTGCTG CTGGTTGGTA CGGGCTTCAC GAAGCAGACA 
GAAGAGCAGT ACTTCCAGTT CCTGGCCAAA TTTGAGATCG CCCTGCCCGT CGCCAATGAC 
AGCTATCTCC TGCCACATCT CCTTCCATCC AAACCGGGCC TGGACACCCA CGGCATGCGG 
CACCCCACAG CCAACACCAT TCAGAGAGTG TTTAAGATGA GCTTCGTCCC GGTTGGCTTC 
TGGCAGCGGT TTATAGCCCG GATGCTGATC AGCTTGGCTG AGATGGACCT CCAGCTGTTT 
GAAAACAGGA AGAATACTAA GAGCAGGCAC AGGAAAGTCA CCATTTATAG TTTTACAGGA 
AACCAGAGAA ATCGTTGCAG CACATTCAGA GTGAAAAGGA ACCAGACCAT CTATTGGCAG 
GAGGGGCTCC TGGTCACGTT TGATGGGGGC TACCTCAGTG TAGAATCCTC GGATGTGAAC 
TGGAAAAAGA AAAAAAGCGG AGGAATAAAA ATCATGTGCC AATCAGAAAT AAGGGACTTC 
TCGGCAATGG CTTTTATCAC AGACCATGTC AATTCCTTGA TCGATCAGTG GTTTCCCGCC 
CTGACGGCCA CGGAAAGTGA CGGGACCCCA CTGATGGAGC AGTATGTGCC CTGCCCTGTC 
TGCGAGGCCT CCTGGGCTCA GCACAGCAAC CCCAGCGAGA GGTCAGAGGA CGTGCAGTAC 
TTCGACATGG AGGACTGCGT GCTGACAGCC ATTGAGCGGG ACTTCATCTC CTGCCCCAGG 
CACCCTGACC TCCTGGTGCC TCTCCAGGAA CTGGTCCCCG AGCTGTTCAT GACCGACTTC 
CCAGCCAGAC TCTTCTTGGA CAACAGCAAG CTGGAGCACA GCGAGGATGA GAGCGCTGTC 
CTGGGCCAAG GTGGGAGTGG CACCGTCATC TACCGGGCCA GGTACCAAGG CCAGCCTGTG 
GCTGTGAAGC GATTCCAGAT CAAGAAATTC AAGAACTATG CAAATGCCCC TGCAGATACC 
ATGCTGAGGC ACCTGCGGGC CACTGACGCC ATGAGGAATT TCTCGGAGTT CCGGCAGGAG 
GCCAGCATGC TGCACGCCCT GCAGCACCCC TGCATCGTGT CCCTCATCGG CATCAGCATC 
CACCCGCTCT GCTTCGCCCT GGAGCTTGCC CCACTGGGCA GCCTCAACAC CGTGCTGTCC 
GAGAACGCCA GAGATTCCTC CTTTATGCCC TTGGGACACA TGCTCACCCA AAAAATAGCC 
TACCAGATAG CCTCTGGCCT GGCCTACCTG CACAAGAAGA ACATCATCTT CTGTGACCTG 
AAGTCCGACA ACATCCTGGT CTGGTCTCTG GACATTAAGG AGCACATCAA CATCAAGCTG 
TCCGACTACG GCATCTCCCG GCAGTCGTTC CACGAGGGTG CCCTTGGCGT GGAGGGCACT 
CCCGGCTACC AGGCCCCGGA GATCCGGCCT CGCATCGTGT ATGACGAGAA GGTAGACATG 
TTCTCCTATG GGATGGTGCT GTACGAGTTG CTGTCAGGAC AGCGGCCCGC ACTGGGCCAG 
CACCAGCTCC AGATCTCCAA GAAGCTGTCC AAGGGCATCC GCCCAGTTCT GGGTCAGCCG 
GAGGAAGTGC AGTTCCACCG ACTGCAGGCG CTTATGATGG AGTGCTGGGA TACGAAGCCC 
GAGAAGCGAC CACTGGCCCT GGCGGTGGTG AGCCAGATGA AGGACCCGAC CTTTGCGACC 
TTCATGTACC AACTGCCCTG TGGGAAGCAG ACGTCCTTCT TCTCATCCCA GGGCCAGGAG 
TACATGGTGG TGTTCTGGGA AGGCAAGGAG GAGTCCAGGA ACTACACGGT GGTGAACACG 
GAGAAGGGCC TTCTGGAAGT GCAGCACATG AGCTGTGCGG GAATGAAGCT GAGCTGCCAA 
CTCAAGATCC AGAACTCGCT GTGGACGGCC ACCGAGGACC AGAAGATCTA CATCTACAGC 
CTCAAGGGCA TGTGTCCACT GAACACACCC CAGCGGGCTT TGGACACCCC GGCCATCGTC 
ACCTGCTTCT TGGCAGTACC TATTATAAAA AAGAACTCCC ACCTGGTGCT AGCAGGCCTG 
GCGGACGGCC TGGTGGCGGT GTTCCCGGCG GCGTGGGGCG CCCCGGACTA CGGCTGCTCC 
TACCTGTGCT CGCACACAGC CAACAGGGCC AAGTTCAGCA TCCCCGACGA GGACGCCCGG 
CAGAACCCTT ACCCCGTGAC GGCCATGGAG GTGGTCAACT GCGGGTCCGA GGTCTGGTAC 
AGCAACGGGC CAGGCCTGCT GGTCATTGAC TGTGCGGCCC TGGAGATCCG CCGGCGGCTG 
GAGCCCTACG CGGCGCCCTC GGTGGTCACG TCGGTGGTGT GCGGCTCGGA GTGCCGTGGC 
GAGGAGGTGG TGTGGTGCCT GGACGATAGG GCCAACTGCC TGGTGCTGTA CCACTCGGCG 
ACCTACCAGC TGTGCGCCCG CTACTTCTGC GGGGACCCCA GCCCCCTAGG GGACGTGTTT 
CCCGTGTGCC CGATGGGCCC ACCGCCCCTG GACAGCCCCA CAGCCAGCAC GAGGGAGCCC 
GCGAGGGGAT CCATCGCCGA CGTGAGCTTC ATGTTCAGCG AGGAGCTGGG CACACAGATC 
CTCACCCACC AGGACTCGCT GACCGACTAC TGTTCCCTAT CCTCCTGCTC CGCGGCCTCG 
CCGCACCCGG CGCCGATGGC CCTGTCCAGC CTGCCCTGCT CCCCGGCCAG CTCTGCCAGC 
TTGCCCTTCT CCTCTGACTG TGAGGACACC AGCCAGCTGC CCAAGCCCGC TGTGGGCTCC 
GACAGGTCGG TGCATGACCT GAGCCCAGTG GACGGCGAGG CGTTCAGCCA GCACCTGCAG 
GCTGTGAAGA TCCTCGCCGT GAAAGACGTC ATCTGGGTCC CCAGGCGCGG TGGAGATATC 
ATCGTCATTA GCCTGGAGAA GGAGGCAGGA ACTCAGCGGG GCCGCGTGAT TGCTGTCTTG 
AAAGCCCGAG AGCTGACTCC GCATGGGGTT CTCACGGAGG CTGCAGTGGT GGCAAAGGAC 
ACGGTTGTGT GCGGCTTTGA GAATGAAAAT CTGGAGTGGT GCCTGGCTGT CTGGAGGGGC 
TGGGGCACGC AGGAGTTTGA CATTTTCTAC CAGTCCTACG AGGAGCTGGG CCGGTTGGAG 
GCCTGCGTGC GGAAGAGGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815189.1
CDS	130..5433
Translation	MMTREPEALRVKWSHLKLPWVDLDWLIDISCQITELDLSANCLASLPSVVPWGLINLRKLNLSDNHLGELPSVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLEKLFEEESATNWIGLRKLQELDVSDNKLTELPALFLHSFKSLSCLNVSRNNLKAFPDAWACPLKCCKASKNALESLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLKLQGNQLVALPPQEKWTCRQLKTLDLSRNHFGKNEDVLKTKRISFFTTRGRQRSGTEAACVLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKSLSELYLGNNPSLRELPPELGQLGNLWQLDIEDLNIINVPAEIRKEGPKAMLSYLRAQLRKAEKCKQMKMIIVGPPRQGKSTLLEILQTGRAPQVVHSEATIRTTKWELQRPAGSRAKVESVEFTVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVASLQFWLLNIEAKAPNAVVLVVGTHLDLIETKFRVERIATLRAYVLALCRSPSGSRATGFPDITCKHLHELSCKSLEGQEGLRQLIFQVTCNMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEKQHRSLSDDVQYLTDRQLEQLVDQMPCSDIQDYEDLQSAISFLIETGSLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRLLLVGTGFTKQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENRKNTKSRHRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIMCQSEIRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHSNPSERSEDVQYFDMEDCVLTAIERDFISCPRHPDLLVPLQELVPELFMTDFPARLFLDNSKLEHSEDESAVLGQGGSGTVIYRARYQGQPVAVKRFQIKKFKNYANAPADTMLRHLRATDAMRNFSEFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENARDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDIKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGQHQLQISKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALAVVSQMKDPTFATFMYQLPCGKQTSFFSSQGQEYMVVFWEGKEESRNYTVVNTEKGLLEVQHMSCAGMKLSCQLKIQNSLWTATEDQKIYIYSLKGMCPLNTPQRALDTPAIVTCFLAVPIIKKNSHLVLAGLADGLVAVFPAAWGAPDYGCSYLCSHTANRAKFSIPDEDARQNPYPVTAMEVVNCGSEVWYSNGPGLLVIDCAALEIRRRLEPYAAPSVVTSVVCGSECRGEEVVWCLDDRANCLVLYHSATYQLCARYFCGDPSPLGDVFPVCPMGPPPLDSPTASTREPARGSIADVSFMFSEELGTQILTHQDSLTDYCSLSSCSAASPHPAPMALSSLPCSPASSASLPFSSDCEDTSQLPKPAVGSDRSVHDLSPVDGEAFSQHLQAVKILAVKDVIWVPRRGGDIIVISLEKEAGTQRGRVIAVLKARELTPHGVLTEAAVVAKDTVVCGFENENLEWCLAVWRGWGTQEFDIFYQSYEELGRLEACVRKRR

Target ORF information:

RefSeq Version	XM_010816887.3
Organism	Bos taurus(cattle)
Definition	Bos taurus leucine rich repeat kinase 1 (LRRK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816887.3

ORF Insert Sequence:

ATGATGACTC GGGAGCCAGA GGCTCTGCGT GTGAAGTGGT CCCACCTCAA GCTGCCCTGG 
GTGGATCTGG ACTGGCTCAT CGACATCTCC TGCCAGATCA CAGAGCTGGA TCTTTCTGCC 
AACTGCCTGG CGTCCCTCCC CTCAGTCGTC CCCTGGGGCC TCATCAACCT CCGGAAGCTG 
AACCTCTCAG ACAACCACCT GGGGGAGCTG CCCAGCGTGC AGTCATCAGA TGAAATCATC 
TGTTCCAGGC TACTTGAAAT TGACATTTCC AGCAACAAGT TGTCCCACCT CCCACCAGGA 
TTCCTGCATC TCTCAAAACT TCAAAAACTG ACGGCTTCAA AAAACTATTT GGAAAAATTG 
TTTGAAGAAG AGAGTGCCAC GAACTGGATT GGTTTACGGA AGCTACAGGA GCTCGATGTT 
TCTGACAACA AACTGACAGA ACTCCCTGCA CTTTTCCTCC ATTCTTTCAA ATCGCTCAGT 
TGTCTAAACG TCTCCAGAAA TAACCTGAAG GCGTTTCCAG ATGCCTGGGC TTGCCCTTTG 
AAATGTTGCA AAGCATCCAA AAATGCCCTG GAGTCTCTAC CAGACAAAAT GGCTGTGTTT 
TGGAAGAACC ACTTGAAGGA TGTGGACTTC TCAGAAAACG CACTGAAAGA AGTTCCCCTG 
GGACTTTTCC AGCTTGATGC CCTCATGTTC TTGAAGTTAC AGGGAAACCA GCTGGTGGCA 
CTCCCACCTC AAGAGAAGTG GACCTGCAGA CAGCTCAAAA CCCTGGATCT CTCCAGAAAT 
CACTTTGGCA AAAATGAAGA CGTCCTTAAA ACAAAGCGCA TTTCCTTTTT CACCACCAGA 
GGCCGCCAGC GTTCGGGGAC GGAGGCAGCG TGTGTACTTG AATTTCCCGC CTTCCTAAGT 
GAGTCTTTGG AAGTCCTCTG TCTGAACGAC AACCACCTGG ATGCAGTCCC GCCCTCAGTT 
TGCCTACTGA AGAGCTTATC AGAACTCTAC TTGGGGAACA ATCCTAGCCT CCGAGAGCTC 
CCTCCTGAGC TGGGGCAGCT GGGGAACCTC TGGCAGTTGG ACATTGAAGA CCTGAATATC 
ATCAACGTGC CTGCAGAGAT CAGAAAAGAA GGCCCCAAAG CAATGCTGTC TTACCTGCGT 
GCTCAGCTGC GGAAAGCAGA GAAGTGCAAG CAGATGAAGA TGATCATCGT GGGTCCGCCG 
CGTCAGGGCA AGTCCACCCT CCTGGAGATC TTACAGACGG GGAGGGCCCC CCAGGTGGTA 
CACAGCGAGG CCACCATCAG GACCACCAAG TGGGAGCTGC AGAGGCCGGC TGGCTCGAGA 
GCCAAGGTTG AGTCTGTGGA GTTCACCGTC TGGGACATCG GCGGCCCCGC CAGCATGGCC 
ACGGTCAACC AGTGCTTCTT CACAGACAAG GCCCTGTACG TGGTGGTCTG GAACCTGGCT 
CTGGGGGAGG AGGCCGTGGC CAGCCTTCAG TTCTGGCTGC TCAACATCGA GGCCAAGGCC 
CCAAATGCTG TGGTGCTGGT GGTGGGAACA CATCTGGACT TAATTGAAAC CAAATTCCGC 
GTGGAGAGGA TCGCCACTCT GCGCGCCTAC GTGCTGGCCC TCTGCCGCTC GCCCTCTGGG 
TCCAGGGCCA CCGGCTTCCC GGACATCACG TGTAAACACC TGCACGAACT CTCCTGTAAG 
AGCTTGGAAG GTCAGGAAGG GCTGCGGCAG CTCATTTTCC AGGTCACGTG CAACATGAAG 
GACGTGGGCA GCACCATCGG CTGTCAGCGA CTTGCAGGGA GGCTGATCCC CAGGAGCTAC 
TTGAGCCTCC AGGAGGCTGT GCTAGCAGAG AAGCAGCACC GCAGCCTGAG TGACGATGTG 
CAGTACCTGA CAGACCGGCA GCTAGAGCAG CTGGTGGACC AGATGCCCTG CAGCGACATC 
CAGGACTACG AGGACCTGCA GTCTGCCATC AGCTTCCTCA TTGAAACGGG CTCCCTGCTG 
CACTTCCCGG ACACCAGCCA CGGCCTGAGG AACCTCTACT TCCTCGACCC CATCTGGCTG 
TCCGAGTGTC TGCAGAGGAT CTTCAACATC AAGGGCTCTC GGTCAGTGGC CAAGAATGGG 
GTGATCAGAG CCGAGGACCT TCGGCTGCTG CTGGTTGGTA CGGGCTTCAC GAAGCAGACA 
GAAGAGCAGT ACTTCCAGTT CCTGGCCAAA TTTGAGATCG CCCTGCCCGT CGCCAATGAC 
AGCTATCTCC TGCCACATCT CCTTCCATCC AAACCGGGCC TGGACACCCA CGGCATGCGG 
CACCCCACAG CCAACACCAT TCAGAGAGTG TTTAAGATGA GCTTCGTCCC GGTTGGCTTC 
TGGCAGCGGT TTATAGCCCG GATGCTGATC AGCTTGGCTG AGATGGACCT CCAGCTGTTT 
GAAAACAGGA AGAATACTAA GAGCAGGCAC AGGAAAGTCA CCATTTATAG TTTTACAGGA 
AACCAGAGAA ATCGTTGCAG CACATTCAGA GTGAAAAGGA ACCAGACCAT CTATTGGCAG 
GAGGGGCTCC TGGTCACGTT TGATGGGGGC TACCTCAGTG TAGAATCCTC GGATGTGAAC 
TGGAAAAAGA AAAAAAGCGG AGGAATAAAA ATCATGTGCC AATCAGAAAT AAGGGACTTC 
TCGGCAATGG CTTTTATCAC AGACCATGTC AATTCCTTGA TCGATCAGTG GTTTCCCGCC 
CTGACGGCCA CGGAAAGTGA CGGGACCCCA CTGATGGAGC AGTATGTGCC CTGCCCTGTC 
TGCGAGGCCT CCTGGGCTCA GCACAGCAAC CCCAGCGAGA GGTCAGAGGA CGTGCAGTAC 
TTCGACATGG AGGACTGCGT GCTGACAGCC ATTGAGCGGG ACTTCATCTC CTGCCCCAGG 
CACCCTGACC TCCTGGTGCC TCTCCAGGAA CTGGTCCCCG AGCTGTTCAT GACCGACTTC 
CCAGCCAGAC TCTTCTTGGA CAACAGCAAG CTGGAGCACA GCGAGGATGA GAGCGCTGTC 
CTGGGCCAAG GTGGGAGTGG CACCGTCATC TACCGGGCCA GGTACCAAGG CCAGCCTGTG 
GCTGTGAAGC GATTCCAGAT CAAGAAATTC AAGAACTATG CAAATGCCCC TGCAGATACC 
ATGCTGAGGC ACCTGCGGGC CACTGACGCC ATGAGGAATT TCTCGGAGTT CCGGCAGGAG 
GCCAGCATGC TGCACGCCCT GCAGCACCCC TGCATCGTGT CCCTCATCGG CATCAGCATC 
CACCCGCTCT GCTTCGCCCT GGAGCTTGCC CCACTGGGCA GCCTCAACAC CGTGCTGTCC 
GAGAACGCCA GAGATTCCTC CTTTATGCCC TTGGGACACA TGCTCACCCA AAAAATAGCC 
TACCAGATAG CCTCTGGCCT GGCCTACCTG CACAAGAAGA ACATCATCTT CTGTGACCTG 
AAGTCCGACA ACATCCTGGT CTGGTCTCTG GACATTAAGG AGCACATCAA CATCAAGCTG 
TCCGACTACG GCATCTCCCG GCAGTCGTTC CACGAGGGTG CCCTTGGCGT GGAGGGCACT 
CCCGGCTACC AGGCCCCGGA GATCCGGCCT CGCATCGTGT ATGACGAGAA GGTAGACATG 
TTCTCCTATG GGATGGTGCT GTACGAGTTG CTGTCAGGAC AGCGGCCCGC ACTGGGCCAG 
CACCAGCTCC AGATCTCCAA GAAGCTGTCC AAGGGCATCC GCCCAGTTCT GGGTCAGCCG 
GAGGAAGTGC AGTTCCACCG ACTGCAGGCG CTTATGATGG AGTGCTGGGA TACGAAGCCC 
GAGAAGCGAC CACTGGCCCT GGCGGTGGTG AGCCAGATGA AGGACCCGAC CTTTGCGACC 
TTCATGTACC AACTGCCCTG TGGGAAGCAG ACGTCCTTCT TCTCATCCCA GGGCCAGGAG 
TACATGGTGG TGTTCTGGGA AGGCAAGGAG GAGTCCAGGA ACTACACGGT GGTGAACACG 
GAGAAGGGCC TTCTGGAAGT GCAGCACATG AGCTGTGCGG GAATGAAGCT GAGCTGCCAA 
CTCAAGATCC AGAACTCGCT GTGGACGGCC ACCGAGGACC AGAAGATCTA CATCTACAGC 
CTCAAGGGCA TGTGTCCACT GAACACACCC CAGCGGGCTT TGGACACCCC GGCCATCGTC 
ACCTGCTTCT TGGCAGTACC TATTATAAAA AAGAACTCCC ACCTGGTGCT AGCAGGCCTG 
GCGGACGGCC TGGTGGCGGT GTTCCCGGCG GCGTGGGGCG CCCCGGACTA CGGCTGCTCC 
TACCTGTGCT CGCACACAGC CAACAGGGCC AAGTTCAGCA TCCCCGACGA GGACGCCCGG 
CAGAACCCTT ACCCCGTGAC GGCCATGGAG GTGGTCAACT GCGGGTCCGA GGTCTGGTAC 
AGCAACGGGC CAGGCCTGCT GGTCATTGAC TGTGCGGCCC TGGAGATCCG CCGGCGGCTG 
GAGCCCTACG CGGCGCCCTC GGTGGTCACG TCGGTGGTGT GCGGCTCGGA GTGCCGTGGC 
GAGGAGGTGG TGTGGTGCCT GGACGATAGG GCCAACTGCC TGGTGCTGTA CCACTCGGCG 
ACCTACCAGC TGTGCGCCCG CTACTTCTGC GGGGACCCCA GCCCCCTAGG GGACGTGTTT 
CCCGTGTGCC CGATGGGCCC ACCGCCCCTG GACAGCCCCA CAGCCAGCAC GAGGGAGCCC 
GCGAGGGGAT CCATCGCCGA CGTGAGCTTC ATGTTCAGCG AGGAGCTGGG CACACAGATC 
CTCACCCACC AGGACTCGCT GACCGACTAC TGTTCCCTAT CCTCCTGCTC CGCGGCCTCG 
CCGCACCCGG CGCCGATGGC CCTGTCCAGC CTGCCCTGCT CCCCGGCCAG CTCTGCCAGC 
TTGCCCTTCT CCTCTGACTG TGAGGACACC AGCCAGCTGC CCAAGCCCGC TGTGGGCTCC 
GACAGGTCGG TGCATGACCT GAGCCCAGTG GACGGCGAGG CGTTCAGCCA GCACCTGCAG 
GCTGTGAAGA TCCTCGCCGT GAAAGACGTC ATCTGGGTCC CCAGGCGCGG TGGAGATATC 
ATCGTCATTA GCCTGGAGAA GGAGGCAGGA ACTCAGCGGG GCCGCGTGAT TGCTGTCTTG 
AAAGCCCGAG AGCTGACTCC GCATGGGGTT CTCACGGAGG CTGCAGTGGT GGCAAAGGAC 
ACGGTTGTGT GCGGCTTTGA GAATGAAAAT CTGGAGTGGT GCCTGGCTGT CTGGAGGGGC 
TGGGGCACGC AGGAGTTTGA CATTTTCTAC CAGTCCTACG AGGAGCTGGG CCGGTTGGAG 
GCCTGCGTGC GGAAGAGGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRK1 ( XM_010816887.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815189.1 Bos taurus leucine-rich repeat kinase 1 (LRRK1), transcript variant X6, mRNA.

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CloneID	OBa48783
Clone ID Related Accession (Same CDS sequence)	XM_010816887.3 , XM_010816887.2
Accession Version	XM_010816887.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	leucine-rich repeat serine/threonine-protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104530.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816887.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACTC GGGAGCCAGA GGCTCTGCGT GTGAAGTGGT CCCACCTCAA GCTGCCCTGG 
GTGGATCTGG ACTGGCTCAT CGACATCTCC TGCCAGATCA CAGAGCTGGA TCTTTCTGCC 
AACTGCCTGG CGTCCCTCCC CTCAGTCGTC CCCTGGGGCC TCATCAACCT CCGGAAGCTG 
AACCTCTCAG ACAACCACCT GGGGGAGCTG CCCAGCGTGC AGTCATCAGA TGAAATCATC 
TGTTCCAGGC TACTTGAAAT TGACATTTCC AGCAACAAGT TGTCCCACCT CCCACCAGGA 
TTCCTGCATC TCTCAAAACT TCAAAAACTG ACGGCTTCAA AAAACTATTT GGAAAAATTG 
TTTGAAGAAG AGAGTGCCAC GAACTGGATT GGTTTACGGA AGCTACAGGA GCTCGATGTT 
TCTGACAACA AACTGACAGA ACTCCCTGCA CTTTTCCTCC ATTCTTTCAA ATCGCTCAGT 
TGTCTAAACG TCTCCAGAAA TAACCTGAAG GCGTTTCCAG ATGCCTGGGC TTGCCCTTTG 
AAATGTTGCA AAGCATCCAA AAATGCCCTG GAGTCTCTAC CAGACAAAAT GGCTGTGTTT 
TGGAAGAACC ACTTGAAGGA TGTGGACTTC TCAGAAAACG CACTGAAAGA AGTTCCCCTG 
GGACTTTTCC AGCTTGATGC CCTCATGTTC TTGAAGTTAC AGGGAAACCA GCTGGTGGCA 
CTCCCACCTC AAGAGAAGTG GACCTGCAGA CAGCTCAAAA CCCTGGATCT CTCCAGAAAT 
CACTTTGGCA AAAATGAAGA CGTCCTTAAA ACAAAGCGCA TTTCCTTTTT CACCACCAGA 
GGCCGCCAGC GTTCGGGGAC GGAGGCAGCG TGTGTACTTG AATTTCCCGC CTTCCTAAGT 
GAGTCTTTGG AAGTCCTCTG TCTGAACGAC AACCACCTGG ATGCAGTCCC GCCCTCAGTT 
TGCCTACTGA AGAGCTTATC AGAACTCTAC TTGGGGAACA ATCCTAGCCT CCGAGAGCTC 
CCTCCTGAGC TGGGGCAGCT GGGGAACCTC TGGCAGTTGG ACATTGAAGA CCTGAATATC 
ATCAACGTGC CTGCAGAGAT CAGAAAAGAA GGCCCCAAAG CAATGCTGTC TTACCTGCGT 
GCTCAGCTGC GGAAAGCAGA GAAGTGCAAG CAGATGAAGA TGATCATCGT GGGTCCGCCG 
CGTCAGGGCA AGTCCACCCT CCTGGAGATC TTACAGACGG GGAGGGCCCC CCAGGTGGTA 
CACAGCGAGG CCACCATCAG GACCACCAAG TGGGAGCTGC AGAGGCCGGC TGGCTCGAGA 
GCCAAGGTTG AGTCTGTGGA GTTCACCGTC TGGGACATCG GCGGCCCCGC CAGCATGGCC 
ACGGTCAACC AGTGCTTCTT CACAGACAAG GCCCTGTACG TGGTGGTCTG GAACCTGGCT 
CTGGGGGAGG AGGCCGTGGC CAGCCTTCAG TTCTGGCTGC TCAACATCGA GGCCAAGGCC 
CCAAATGCTG TGGTGCTGGT GGTGGGAACA CATCTGGACT TAATTGAAAC CAAATTCCGC 
GTGGAGAGGA TCGCCACTCT GCGCGCCTAC GTGCTGGCCC TCTGCCGCTC GCCCTCTGGG 
TCCAGGGCCA CCGGCTTCCC GGACATCACG TGTAAACACC TGCACGAACT CTCCTGTAAG 
AGCTTGGAAG GTCAGGAAGG GCTGCGGCAG CTCATTTTCC AGGTCACGTG CAACATGAAG 
GACGTGGGCA GCACCATCGG CTGTCAGCGA CTTGCAGGGA GGCTGATCCC CAGGAGCTAC 
TTGAGCCTCC AGGAGGCTGT GCTAGCAGAG AAGCAGCACC GCAGCCTGAG TGACGATGTG 
CAGTACCTGA CAGACCGGCA GCTAGAGCAG CTGGTGGACC AGATGCCCTG CAGCGACATC 
CAGGACTACG AGGACCTGCA GTCTGCCATC AGCTTCCTCA TTGAAACGGG CTCCCTGCTG 
CACTTCCCGG ACACCAGCCA CGGCCTGAGG AACCTCTACT TCCTCGACCC CATCTGGCTG 
TCCGAGTGTC TGCAGAGGAT CTTCAACATC AAGGGCTCTC GGTCAGTGGC CAAGAATGGG 
GTGATCAGAG CCGAGGACCT TCGGCTGCTG CTGGTTGGTA CGGGCTTCAC GAAGCAGACA 
GAAGAGCAGT ACTTCCAGTT CCTGGCCAAA TTTGAGATCG CCCTGCCCGT CGCCAATGAC 
AGCTATCTCC TGCCACATCT CCTTCCATCC AAACCGGGCC TGGACACCCA CGGCATGCGG 
CACCCCACAG CCAACACCAT TCAGAGAGTG TTTAAGATGA GCTTCGTCCC GGTTGGCTTC 
TGGCAGCGGT TTATAGCCCG GATGCTGATC AGCTTGGCTG AGATGGACCT CCAGCTGTTT 
GAAAACAGGA AGAATACTAA GAGCAGGCAC AGGAAAGTCA CCATTTATAG TTTTACAGGA 
AACCAGAGAA ATCGTTGCAG CACATTCAGA GTGAAAAGGA ACCAGACCAT CTATTGGCAG 
GAGGGGCTCC TGGTCACGTT TGATGGGGGC TACCTCAGTG TAGAATCCTC GGATGTGAAC 
TGGAAAAAGA AAAAAAGCGG AGGAATAAAA ATCATGTGCC AATCAGAAAT AAGGGACTTC 
TCGGCAATGG CTTTTATCAC AGACCATGTC AATTCCTTGA TCGATCAGTG GTTTCCCGCC 
CTGACGGCCA CGGAAAGTGA CGGGACCCCA CTGATGGAGC AGTATGTGCC CTGCCCTGTC 
TGCGAGGCCT CCTGGGCTCA GCACAGCAAC CCCAGCGAGA GGTCAGAGGA CGTGCAGTAC 
TTCGACATGG AGGACTGCGT GCTGACAGCC ATTGAGCGGG ACTTCATCTC CTGCCCCAGG 
CACCCTGACC TCCTGGTGCC TCTCCAGGAA CTGGTCCCCG AGCTGTTCAT GACCGACTTC 
CCAGCCAGAC TCTTCTTGGA CAACAGCAAG CTGGAGCACA GCGAGGATGA GAGCGCTGTC 
CTGGGCCAAG GTGGGAGTGG CACCGTCATC TACCGGGCCA GGTACCAAGG CCAGCCTGTG 
GCTGTGAAGC GATTCCAGAT CAAGAAATTC AAGAACTATG CAAATGCCCC TGCAGATACC 
ATGCTGAGGC ACCTGCGGGC CACTGACGCC ATGAGGAATT TCTCGGAGTT CCGGCAGGAG 
GCCAGCATGC TGCACGCCCT GCAGCACCCC TGCATCGTGT CCCTCATCGG CATCAGCATC 
CACCCGCTCT GCTTCGCCCT GGAGCTTGCC CCACTGGGCA GCCTCAACAC CGTGCTGTCC 
GAGAACGCCA GAGATTCCTC CTTTATGCCC TTGGGACACA TGCTCACCCA AAAAATAGCC 
TACCAGATAG CCTCTGGCCT GGCCTACCTG CACAAGAAGA ACATCATCTT CTGTGACCTG 
AAGTCCGACA ACATCCTGGT CTGGTCTCTG GACATTAAGG AGCACATCAA CATCAAGCTG 
TCCGACTACG GCATCTCCCG GCAGTCGTTC CACGAGGGTG CCCTTGGCGT GGAGGGCACT 
CCCGGCTACC AGGCCCCGGA GATCCGGCCT CGCATCGTGT ATGACGAGAA GGTAGACATG 
TTCTCCTATG GGATGGTGCT GTACGAGTTG CTGTCAGGAC AGCGGCCCGC ACTGGGCCAG 
CACCAGCTCC AGATCTCCAA GAAGCTGTCC AAGGGCATCC GCCCAGTTCT GGGTCAGCCG 
GAGGAAGTGC AGTTCCACCG ACTGCAGGCG CTTATGATGG AGTGCTGGGA TACGAAGCCC 
GAGAAGCGAC CACTGGCCCT GGCGGTGGTG AGCCAGATGA AGGACCCGAC CTTTGCGACC 
TTCATGTACC AACTGCCCTG TGGGAAGCAG ACGTCCTTCT TCTCATCCCA GGGCCAGGAG 
TACATGGTGG TGTTCTGGGA AGGCAAGGAG GAGTCCAGGA ACTACACGGT GGTGAACACG 
GAGAAGGGCC TTCTGGAAGT GCAGCACATG AGCTGTGCGG GAATGAAGCT GAGCTGCCAA 
CTCAAGATCC AGAACTCGCT GTGGACGGCC ACCGAGGACC AGAAGATCTA CATCTACAGC 
CTCAAGGGCA TGTGTCCACT GAACACACCC CAGCGGGCTT TGGACACCCC GGCCATCGTC 
ACCTGCTTCT TGGCAGTACC TATTATAAAA AAGAACTCCC ACCTGGTGCT AGCAGGCCTG 
GCGGACGGCC TGGTGGCGGT GTTCCCGGCG GCGTGGGGCG CCCCGGACTA CGGCTGCTCC 
TACCTGTGCT CGCACACAGC CAACAGGGCC AAGTTCAGCA TCCCCGACGA GGACGCCCGG 
CAGAACCCTT ACCCCGTGAC GGCCATGGAG GTGGTCAACT GCGGGTCCGA GGTCTGGTAC 
AGCAACGGGC CAGGCCTGCT GGTCATTGAC TGTGCGGCCC TGGAGATCCG CCGGCGGCTG 
GAGCCCTACG CGGCGCCCTC GGTGGTCACG TCGGTGGTGT GCGGCTCGGA GTGCCGTGGC 
GAGGAGGTGG TGTGGTGCCT GGACGATAGG GCCAACTGCC TGGTGCTGTA CCACTCGGCG 
ACCTACCAGC TGTGCGCCCG CTACTTCTGC GGGGACCCCA GCCCCCTAGG GGACGTGTTT 
CCCGTGTGCC CGATGGGCCC ACCGCCCCTG GACAGCCCCA CAGCCAGCAC GAGGGAGCCC 
GCGAGGGGAT CCATCGCCGA CGTGAGCTTC ATGTTCAGCG AGGAGCTGGG CACACAGATC 
CTCACCCACC AGGACTCGCT GACCGACTAC TGTTCCCTAT CCTCCTGCTC CGCGGCCTCG 
CCGCACCCGG CGCCGATGGC CCTGTCCAGC CTGCCCTGCT CCCCGGCCAG CTCTGCCAGC 
TTGCCCTTCT CCTCTGACTG TGAGGACACC AGCCAGCTGC CCAAGCCCGC TGTGGGCTCC 
GACAGGTCGG TGCATGACCT GAGCCCAGTG GACGGCGAGG CGTTCAGCCA GCACCTGCAG 
GCTGTGAAGA TCCTCGCCGT GAAAGACGTC ATCTGGGTCC CCAGGCGCGG TGGAGATATC 
ATCGTCATTA GCCTGGAGAA GGAGGCAGGA ACTCAGCGGG GCCGCGTGAT TGCTGTCTTG 
AAAGCCCGAG AGCTGACTCC GCATGGGGTT CTCACGGAGG CTGCAGTGGT GGCAAAGGAC 
ACGGTTGTGT GCGGCTTTGA GAATGAAAAT CTGGAGTGGT GCCTGGCTGT CTGGAGGGGC 
TGGGGCACGC AGGAGTTTGA CATTTTCTAC CAGTCCTACG AGGAGCTGGG CCGGTTGGAG 
GCCTGCGTGC GGAAGAGGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815189.1
CDS	448..5751
Translation	MMTREPEALRVKWSHLKLPWVDLDWLIDISCQITELDLSANCLASLPSVVPWGLINLRKLNLSDNHLGELPSVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLEKLFEEESATNWIGLRKLQELDVSDNKLTELPALFLHSFKSLSCLNVSRNNLKAFPDAWACPLKCCKASKNALESLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLKLQGNQLVALPPQEKWTCRQLKTLDLSRNHFGKNEDVLKTKRISFFTTRGRQRSGTEAACVLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKSLSELYLGNNPSLRELPPELGQLGNLWQLDIEDLNIINVPAEIRKEGPKAMLSYLRAQLRKAEKCKQMKMIIVGPPRQGKSTLLEILQTGRAPQVVHSEATIRTTKWELQRPAGSRAKVESVEFTVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVASLQFWLLNIEAKAPNAVVLVVGTHLDLIETKFRVERIATLRAYVLALCRSPSGSRATGFPDITCKHLHELSCKSLEGQEGLRQLIFQVTCNMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEKQHRSLSDDVQYLTDRQLEQLVDQMPCSDIQDYEDLQSAISFLIETGSLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRLLLVGTGFTKQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENRKNTKSRHRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIMCQSEIRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHSNPSERSEDVQYFDMEDCVLTAIERDFISCPRHPDLLVPLQELVPELFMTDFPARLFLDNSKLEHSEDESAVLGQGGSGTVIYRARYQGQPVAVKRFQIKKFKNYANAPADTMLRHLRATDAMRNFSEFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENARDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDIKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGQHQLQISKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALAVVSQMKDPTFATFMYQLPCGKQTSFFSSQGQEYMVVFWEGKEESRNYTVVNTEKGLLEVQHMSCAGMKLSCQLKIQNSLWTATEDQKIYIYSLKGMCPLNTPQRALDTPAIVTCFLAVPIIKKNSHLVLAGLADGLVAVFPAAWGAPDYGCSYLCSHTANRAKFSIPDEDARQNPYPVTAMEVVNCGSEVWYSNGPGLLVIDCAALEIRRRLEPYAAPSVVTSVVCGSECRGEEVVWCLDDRANCLVLYHSATYQLCARYFCGDPSPLGDVFPVCPMGPPPLDSPTASTREPARGSIADVSFMFSEELGTQILTHQDSLTDYCSLSSCSAASPHPAPMALSSLPCSPASSASLPFSSDCEDTSQLPKPAVGSDRSVHDLSPVDGEAFSQHLQAVKILAVKDVIWVPRRGGDIIVISLEKEAGTQRGRVIAVLKARELTPHGVLTEAAVVAKDTVVCGFENENLEWCLAVWRGWGTQEFDIFYQSYEELGRLEACVRKRR

Target ORF information:

RefSeq Version	XM_010816887.2
Organism	Bos taurus(cattle)
Definition	Bos taurus leucine-rich repeat kinase 1 (LRRK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816887.2

ORF Insert Sequence:

ATGATGACTC GGGAGCCAGA GGCTCTGCGT GTGAAGTGGT CCCACCTCAA GCTGCCCTGG 
GTGGATCTGG ACTGGCTCAT CGACATCTCC TGCCAGATCA CAGAGCTGGA TCTTTCTGCC 
AACTGCCTGG CGTCCCTCCC CTCAGTCGTC CCCTGGGGCC TCATCAACCT CCGGAAGCTG 
AACCTCTCAG ACAACCACCT GGGGGAGCTG CCCAGCGTGC AGTCATCAGA TGAAATCATC 
TGTTCCAGGC TACTTGAAAT TGACATTTCC AGCAACAAGT TGTCCCACCT CCCACCAGGA 
TTCCTGCATC TCTCAAAACT TCAAAAACTG ACGGCTTCAA AAAACTATTT GGAAAAATTG 
TTTGAAGAAG AGAGTGCCAC GAACTGGATT GGTTTACGGA AGCTACAGGA GCTCGATGTT 
TCTGACAACA AACTGACAGA ACTCCCTGCA CTTTTCCTCC ATTCTTTCAA ATCGCTCAGT 
TGTCTAAACG TCTCCAGAAA TAACCTGAAG GCGTTTCCAG ATGCCTGGGC TTGCCCTTTG 
AAATGTTGCA AAGCATCCAA AAATGCCCTG GAGTCTCTAC CAGACAAAAT GGCTGTGTTT 
TGGAAGAACC ACTTGAAGGA TGTGGACTTC TCAGAAAACG CACTGAAAGA AGTTCCCCTG 
GGACTTTTCC AGCTTGATGC CCTCATGTTC TTGAAGTTAC AGGGAAACCA GCTGGTGGCA 
CTCCCACCTC AAGAGAAGTG GACCTGCAGA CAGCTCAAAA CCCTGGATCT CTCCAGAAAT 
CACTTTGGCA AAAATGAAGA CGTCCTTAAA ACAAAGCGCA TTTCCTTTTT CACCACCAGA 
GGCCGCCAGC GTTCGGGGAC GGAGGCAGCG TGTGTACTTG AATTTCCCGC CTTCCTAAGT 
GAGTCTTTGG AAGTCCTCTG TCTGAACGAC AACCACCTGG ATGCAGTCCC GCCCTCAGTT 
TGCCTACTGA AGAGCTTATC AGAACTCTAC TTGGGGAACA ATCCTAGCCT CCGAGAGCTC 
CCTCCTGAGC TGGGGCAGCT GGGGAACCTC TGGCAGTTGG ACATTGAAGA CCTGAATATC 
ATCAACGTGC CTGCAGAGAT CAGAAAAGAA GGCCCCAAAG CAATGCTGTC TTACCTGCGT 
GCTCAGCTGC GGAAAGCAGA GAAGTGCAAG CAGATGAAGA TGATCATCGT GGGTCCGCCG 
CGTCAGGGCA AGTCCACCCT CCTGGAGATC TTACAGACGG GGAGGGCCCC CCAGGTGGTA 
CACAGCGAGG CCACCATCAG GACCACCAAG TGGGAGCTGC AGAGGCCGGC TGGCTCGAGA 
GCCAAGGTTG AGTCTGTGGA GTTCACCGTC TGGGACATCG GCGGCCCCGC CAGCATGGCC 
ACGGTCAACC AGTGCTTCTT CACAGACAAG GCCCTGTACG TGGTGGTCTG GAACCTGGCT 
CTGGGGGAGG AGGCCGTGGC CAGCCTTCAG TTCTGGCTGC TCAACATCGA GGCCAAGGCC 
CCAAATGCTG TGGTGCTGGT GGTGGGAACA CATCTGGACT TAATTGAAAC CAAATTCCGC 
GTGGAGAGGA TCGCCACTCT GCGCGCCTAC GTGCTGGCCC TCTGCCGCTC GCCCTCTGGG 
TCCAGGGCCA CCGGCTTCCC GGACATCACG TGTAAACACC TGCACGAACT CTCCTGTAAG 
AGCTTGGAAG GTCAGGAAGG GCTGCGGCAG CTCATTTTCC AGGTCACGTG CAACATGAAG 
GACGTGGGCA GCACCATCGG CTGTCAGCGA CTTGCAGGGA GGCTGATCCC CAGGAGCTAC 
TTGAGCCTCC AGGAGGCTGT GCTAGCAGAG AAGCAGCACC GCAGCCTGAG TGACGATGTG 
CAGTACCTGA CAGACCGGCA GCTAGAGCAG CTGGTGGACC AGATGCCCTG CAGCGACATC 
CAGGACTACG AGGACCTGCA GTCTGCCATC AGCTTCCTCA TTGAAACGGG CTCCCTGCTG 
CACTTCCCGG ACACCAGCCA CGGCCTGAGG AACCTCTACT TCCTCGACCC CATCTGGCTG 
TCCGAGTGTC TGCAGAGGAT CTTCAACATC AAGGGCTCTC GGTCAGTGGC CAAGAATGGG 
GTGATCAGAG CCGAGGACCT TCGGCTGCTG CTGGTTGGTA CGGGCTTCAC GAAGCAGACA 
GAAGAGCAGT ACTTCCAGTT CCTGGCCAAA TTTGAGATCG CCCTGCCCGT CGCCAATGAC 
AGCTATCTCC TGCCACATCT CCTTCCATCC AAACCGGGCC TGGACACCCA CGGCATGCGG 
CACCCCACAG CCAACACCAT TCAGAGAGTG TTTAAGATGA GCTTCGTCCC GGTTGGCTTC 
TGGCAGCGGT TTATAGCCCG GATGCTGATC AGCTTGGCTG AGATGGACCT CCAGCTGTTT 
GAAAACAGGA AGAATACTAA GAGCAGGCAC AGGAAAGTCA CCATTTATAG TTTTACAGGA 
AACCAGAGAA ATCGTTGCAG CACATTCAGA GTGAAAAGGA ACCAGACCAT CTATTGGCAG 
GAGGGGCTCC TGGTCACGTT TGATGGGGGC TACCTCAGTG TAGAATCCTC GGATGTGAAC 
TGGAAAAAGA AAAAAAGCGG AGGAATAAAA ATCATGTGCC AATCAGAAAT AAGGGACTTC 
TCGGCAATGG CTTTTATCAC AGACCATGTC AATTCCTTGA TCGATCAGTG GTTTCCCGCC 
CTGACGGCCA CGGAAAGTGA CGGGACCCCA CTGATGGAGC AGTATGTGCC CTGCCCTGTC 
TGCGAGGCCT CCTGGGCTCA GCACAGCAAC CCCAGCGAGA GGTCAGAGGA CGTGCAGTAC 
TTCGACATGG AGGACTGCGT GCTGACAGCC ATTGAGCGGG ACTTCATCTC CTGCCCCAGG 
CACCCTGACC TCCTGGTGCC TCTCCAGGAA CTGGTCCCCG AGCTGTTCAT GACCGACTTC 
CCAGCCAGAC TCTTCTTGGA CAACAGCAAG CTGGAGCACA GCGAGGATGA GAGCGCTGTC 
CTGGGCCAAG GTGGGAGTGG CACCGTCATC TACCGGGCCA GGTACCAAGG CCAGCCTGTG 
GCTGTGAAGC GATTCCAGAT CAAGAAATTC AAGAACTATG CAAATGCCCC TGCAGATACC 
ATGCTGAGGC ACCTGCGGGC CACTGACGCC ATGAGGAATT TCTCGGAGTT CCGGCAGGAG 
GCCAGCATGC TGCACGCCCT GCAGCACCCC TGCATCGTGT CCCTCATCGG CATCAGCATC 
CACCCGCTCT GCTTCGCCCT GGAGCTTGCC CCACTGGGCA GCCTCAACAC CGTGCTGTCC 
GAGAACGCCA GAGATTCCTC CTTTATGCCC TTGGGACACA TGCTCACCCA AAAAATAGCC 
TACCAGATAG CCTCTGGCCT GGCCTACCTG CACAAGAAGA ACATCATCTT CTGTGACCTG 
AAGTCCGACA ACATCCTGGT CTGGTCTCTG GACATTAAGG AGCACATCAA CATCAAGCTG 
TCCGACTACG GCATCTCCCG GCAGTCGTTC CACGAGGGTG CCCTTGGCGT GGAGGGCACT 
CCCGGCTACC AGGCCCCGGA GATCCGGCCT CGCATCGTGT ATGACGAGAA GGTAGACATG 
TTCTCCTATG GGATGGTGCT GTACGAGTTG CTGTCAGGAC AGCGGCCCGC ACTGGGCCAG 
CACCAGCTCC AGATCTCCAA GAAGCTGTCC AAGGGCATCC GCCCAGTTCT GGGTCAGCCG 
GAGGAAGTGC AGTTCCACCG ACTGCAGGCG CTTATGATGG AGTGCTGGGA TACGAAGCCC 
GAGAAGCGAC CACTGGCCCT GGCGGTGGTG AGCCAGATGA AGGACCCGAC CTTTGCGACC 
TTCATGTACC AACTGCCCTG TGGGAAGCAG ACGTCCTTCT TCTCATCCCA GGGCCAGGAG 
TACATGGTGG TGTTCTGGGA AGGCAAGGAG GAGTCCAGGA ACTACACGGT GGTGAACACG 
GAGAAGGGCC TTCTGGAAGT GCAGCACATG AGCTGTGCGG GAATGAAGCT GAGCTGCCAA 
CTCAAGATCC AGAACTCGCT GTGGACGGCC ACCGAGGACC AGAAGATCTA CATCTACAGC 
CTCAAGGGCA TGTGTCCACT GAACACACCC CAGCGGGCTT TGGACACCCC GGCCATCGTC 
ACCTGCTTCT TGGCAGTACC TATTATAAAA AAGAACTCCC ACCTGGTGCT AGCAGGCCTG 
GCGGACGGCC TGGTGGCGGT GTTCCCGGCG GCGTGGGGCG CCCCGGACTA CGGCTGCTCC 
TACCTGTGCT CGCACACAGC CAACAGGGCC AAGTTCAGCA TCCCCGACGA GGACGCCCGG 
CAGAACCCTT ACCCCGTGAC GGCCATGGAG GTGGTCAACT GCGGGTCCGA GGTCTGGTAC 
AGCAACGGGC CAGGCCTGCT GGTCATTGAC TGTGCGGCCC TGGAGATCCG CCGGCGGCTG 
GAGCCCTACG CGGCGCCCTC GGTGGTCACG TCGGTGGTGT GCGGCTCGGA GTGCCGTGGC 
GAGGAGGTGG TGTGGTGCCT GGACGATAGG GCCAACTGCC TGGTGCTGTA CCACTCGGCG 
ACCTACCAGC TGTGCGCCCG CTACTTCTGC GGGGACCCCA GCCCCCTAGG GGACGTGTTT 
CCCGTGTGCC CGATGGGCCC ACCGCCCCTG GACAGCCCCA CAGCCAGCAC GAGGGAGCCC 
GCGAGGGGAT CCATCGCCGA CGTGAGCTTC ATGTTCAGCG AGGAGCTGGG CACACAGATC 
CTCACCCACC AGGACTCGCT GACCGACTAC TGTTCCCTAT CCTCCTGCTC CGCGGCCTCG 
CCGCACCCGG CGCCGATGGC CCTGTCCAGC CTGCCCTGCT CCCCGGCCAG CTCTGCCAGC 
TTGCCCTTCT CCTCTGACTG TGAGGACACC AGCCAGCTGC CCAAGCCCGC TGTGGGCTCC 
GACAGGTCGG TGCATGACCT GAGCCCAGTG GACGGCGAGG CGTTCAGCCA GCACCTGCAG 
GCTGTGAAGA TCCTCGCCGT GAAAGACGTC ATCTGGGTCC CCAGGCGCGG TGGAGATATC 
ATCGTCATTA GCCTGGAGAA GGAGGCAGGA ACTCAGCGGG GCCGCGTGAT TGCTGTCTTG 
AAAGCCCGAG AGCTGACTCC GCATGGGGTT CTCACGGAGG CTGCAGTGGT GGCAAAGGAC 
ACGGTTGTGT GCGGCTTTGA GAATGAAAAT CTGGAGTGGT GCCTGGCTGT CTGGAGGGGC 
TGGGGCACGC AGGAGTTTGA CATTTTCTAC CAGTCCTACG AGGAGCTGGG CCGGTTGGAG 
GCCTGCGTGC GGAAGAGGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRK1 ( NM_001205774.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192703.1 Bos taurus leucine rich repeat kinase 1 (LRRK1), mRNA.

Price & Availability↑

CloneID	OBa30787
Clone ID Related Accession (Same CDS sequence)	XM_005221724.3 , XM_015459145.1 , XM_010816886.2 , XM_005221725.3 , XM_024981744.1 , XM_005221726.4 , NM_001205774.2 , NM_001205774.1 , XM_005221726.3 , XM_024981743.1 , XM_005221725.4 , XM_005221724.4
Accession Version	NM_001205774.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-22
Organism	Bos taurus(cattle)
Product	leucine-rich repeat serine/threonine-protein kinase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000021.1. On Jun 29, 2017 this sequence version replaced NM_001205774.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR6764281.36759.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCATACA GGCGAGGAAA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTAG AGCTACCGAC GGAGCCCACT 
GACGACAACC CCGCTGTGGT GGCAGCACAG TTTGGGCACA CTGATGTCGT TCAGGAGCTA 
CTCGAGTCTT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAACTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCTGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTACGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT GCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCAGT CGTCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAC AACAAACTGA CAGAACTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTA AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACTTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAGCTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAATCACTTT GGCAAAAATG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTTGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCGCCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAACT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCATCAAC GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACAGA CAAGGCCCTG 
TACGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCCG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAT GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGCGTGGAG AGGATCGCCA CTCTGCGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTAGC AGAGAAGCAG 
CACCGCAGCC TGAGTGACGA TGTGCAGTAC CTGACAGACC GGCAGCTAGA GCAGCTGGTG 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGACC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTCG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA TGGGGTGATC AGAGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGTACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTGC CCGTCGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCG 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG CGGTTTATAG CCCGGATGCT GATCAGCTTG 
GCTGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CGTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCCTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGACGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCTGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGACTCTTCT TGGACAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGCGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TATGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AATTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCCTGGAGCT TGCCCCACTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAGAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATT 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC GTTCCACGAG 
GGTGCCCTTG GCGTGGAGGG CACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTGTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTCCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAG 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGTGGGAA GCAGACGTCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCAG TACCTATTAT AAAAAAGAAC 
TCCCACCTGG TGCTAGCAGG CCTGGCGGAC GGCCTGGTGG CGGTGTTCCC GGCGGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCTTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCGGCGC CCTCGGTGGT CACGTCGGTG 
GTGTGCGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TGTACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCGCC CCTGGACAGC 
CCCACAGCCA GCACGAGGGA GCCCGCGAGG GGATCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACACA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCGCAC CCGGCGCCGA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGTGAGGA CACCAGCCAG 
CTGCCCAAGC CCGCTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTCG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCGCG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCATGG GGTTCTCACG 
GAGGCTGCAG TGGTGGCAAA GGACACGGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ACGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGTT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192703.1
CDS	156..6203
Translation	MAGMSRLPPSMYWCVGPAGSAVCPGRAMETQENGAGDMGSKLSMPGSEPMVQSPRTEGIHAAYRRGNISRAQHLLREACDESTSQLEKGQLLSISAAHGDLETVRYLLTERLVELPTEPTDDNPAVVAAQFGHTDVVQELLESLPGPCSPQRLLNWMLALACQQGHLAVVKLLVLTYGADPESYAVRKNEFPVIVRLPLYAAIKSGNEDIAIFLIRHGAFFCSYILLDSPDPSKHLLRKYFVEASALPSSCPGKMALRVKWSHLKLPWVDLDWLIDISCQITELDLSANCLASLPSVVPWGLINLRKLNLSDNHLGELPSVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLEKLFEEESATNWIGLRKLQELDVSDNKLTELPALFLHSFKSLSCLNVSRNNLKAFPDAWACPLKCCKASKNALESLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLKLQGNQLVALPPQEKWTCRQLKTLDLSRNHFGKNEDVLKTKRISFFTTRGRQRSGTEAACVLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKSLSELYLGNNPSLRELPPELGQLGNLWQLDIEDLNIINVPAEIRKEGPKAMLSYLRAQLRKAEKCKQMKMIIVGPPRQGKSTLLEILQTGRAPQVVHSEATIRTTKWELQRPAGSRAKVESVEFTVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVASLQFWLLNIEAKAPNAVVLVVGTHLDLIETKFRVERIATLRAYVLALCRSPSGSRATGFPDITCKHLHELSCKSLEGQEGLRQLIFQVTCNMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEKQHRSLSDDVQYLTDRQLEQLVDQMPCSDIQDYEDLQSAISFLIETGSLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRLLLVGTGFTKQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENRKNTKSRHRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIMCQSEIRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHSNPSERSEDVQYFDMEDCVLTAIERDFISCPRHPDLLVPLQELVPELFMTDFPARLFLDNSKLEHSEDESAVLGQGGSGTVIYRARYQGQPVAVKRFQIKKFKNYANAPADTMLRHLRATDAMRNFSEFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENARDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDIKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGQHQLQISKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALAVVSQMKDPTFATFMYQLPCGKQTSFFSSQGQEYMVVFWEGKEESRNYTVVNTEKGLLEVQHMSCAGMKLSCQLKIQNSLWTATEDQKIYIYSLKGMCPLNTPQRALDTPAIVTCFLAVPIIKKNSHLVLAGLADGLVAVFPAAWGAPDYGCSYLCSHTANRAKFSIPDEDARQNPYPVTAMEVVNCGSEVWYSNGPGLLVIDCAALEIRRRLEPYAAPSVVTSVVCGSECRGEEVVWCLDDRANCLVLYHSATYQLCARYFCGDPSPLGDVFPVCPMGPPPLDSPTASTREPARGSIADVSFMFSEELGTQILTHQDSLTDYCSLSSCSAASPHPAPMALSSLPCSPASSASLPFSSDCEDTSQLPKPAVGSDRSVHDLSPVDGEAFSQHLQAVKILAVKDVIWVPRRGGDIIVISLEKEAGTQRGRVIAVLKARELTPHGVLTEAAVVAKDTVVCGFENENLEWCLAVWRGWGTQEFDIFYQSYEELGRLEACVRKRR

Target ORF information:

RefSeq Version	NM_001205774.2
Organism	Bos taurus(cattle)
Definition	Bos taurus leucine rich repeat kinase 1 (LRRK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205774.2

ORF Insert Sequence:

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCATACA GGCGAGGAAA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTAG AGCTACCGAC GGAGCCCACT 
GACGACAACC CCGCTGTGGT GGCAGCACAG TTTGGGCACA CTGATGTCGT TCAGGAGCTA 
CTCGAGTCTT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAACTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCTGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTACGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT GCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCAGT CGTCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAC AACAAACTGA CAGAACTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTA AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACTTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAGCTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAATCACTTT GGCAAAAATG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTTGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCGCCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAACT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCATCAAC GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACAGA CAAGGCCCTG 
TACGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCCG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAT GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGCGTGGAG AGGATCGCCA CTCTGCGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTAGC AGAGAAGCAG 
CACCGCAGCC TGAGTGACGA TGTGCAGTAC CTGACAGACC GGCAGCTAGA GCAGCTGGTG 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGACC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTCG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA TGGGGTGATC AGAGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGTACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTGC CCGTCGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCG 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG CGGTTTATAG CCCGGATGCT GATCAGCTTG 
GCTGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CGTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCCTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGACGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCTGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGACTCTTCT TGGACAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGCGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TATGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AATTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCCTGGAGCT TGCCCCACTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAGAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATT 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC GTTCCACGAG 
GGTGCCCTTG GCGTGGAGGG CACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTGTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTCCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAG 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGTGGGAA GCAGACGTCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCAG TACCTATTAT AAAAAAGAAC 
TCCCACCTGG TGCTAGCAGG CCTGGCGGAC GGCCTGGTGG CGGTGTTCCC GGCGGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCTTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCGGCGC CCTCGGTGGT CACGTCGGTG 
GTGTGCGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TGTACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCGCC CCTGGACAGC 
CCCACAGCCA GCACGAGGGA GCCCGCGAGG GGATCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACACA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCGCAC CCGGCGCCGA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGTGAGGA CACCAGCCAG 
CTGCCCAAGC CCGCTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTCG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCGCG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCATGG GGTTCTCACG 
GAGGCTGCAG TGGTGGCAAA GGACACGGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ACGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGTT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRK1 ( NM_001205774.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192703.1 Bos taurus leucine-rich repeat kinase 1 (LRRK1), mRNA.

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CloneID	OBa30787
Clone ID Related Accession (Same CDS sequence)	XM_005221724.3 , XM_015459145.1 , XM_010816886.2 , XM_005221725.3 , XM_024981744.1 , XM_005221726.4 , NM_001205774.2 , NM_001205774.1 , XM_005221726.3 , XM_024981743.1 , XM_005221725.4 , XM_005221724.4
Accession Version	NM_001205774.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(Cattle)
Product	leucine-rich repeat serine/threonine-protein kinase 1
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03037764.1, AAFC03121311.1 and AAFC03026010.1. On Apr 20, 2011 this sequence version replaced XM_591774.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCATACA GGCGAGGAAA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTAG AGCTACCGAC GGAGCCCACT 
GACGACAACC CCGCTGTGGT GGCAGCACAG TTTGGGCACA CTGATGTCGT TCAGGAGCTA 
CTCGAGTCTT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAACTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCTGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTACGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT GCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCAGT CGTCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAC AACAAACTGA CAGAACTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTA AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACTTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAGCTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAATCACTTT GGCAAAAATG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTTGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCGCCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAACT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCATCAAC GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACAGA CAAGGCCCTG 
TACGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCCG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAT GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGCGTGGAG AGGATCGCCA CTCTGCGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTAGC AGAGAAGCAG 
CACCGCAGCC TGAGTGACGA TGTGCAGTAC CTGACAGACC GGCAGCTAGA GCAGCTGGTG 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGACC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTCG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA TGGGGTGATC AGAGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGTACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTGC CCGTCGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCG 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG CGGTTTATAG CCCGGATGCT GATCAGCTTG 
GCTGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CGTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCCTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGACGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCTGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGACTCTTCT TGGACAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGCGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TATGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AATTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCCTGGAGCT TGCCCCACTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAGAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATT 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC GTTCCACGAG 
GGTGCCCTTG GCGTGGAGGG CACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTGTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTCCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAG 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGTGGGAA GCAGACGTCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCAG TACCTATTAT AAAAAAGAAC 
TCCCACCTGG TGCTAGCAGG CCTGGCGGAC GGCCTGGTGG CGGTGTTCCC GGCGGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCTTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCGGCGC CCTCGGTGGT CACGTCGGTG 
GTGTGCGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TGTACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCGCC CCTGGACAGC 
CCCACAGCCA GCACGAGGGA GCCCGCGAGG GGATCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACACA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCGCAC CCGGCGCCGA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGTGAGGA CACCAGCCAG 
CTGCCCAAGC CCGCTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTCG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCGCG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCATGG GGTTCTCACG 
GAGGCTGCAG TGGTGGCAAA GGACACGGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ACGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGTT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192703.1
CDS	157..6204
Translation	MAGMSRLPPSMYWCVGPAGSAVCPGRAMETQENGAGDMGSKLSMPGSEPMVQSPRTEGIHAAYRRGNISRAQHLLREACDESTSQLEKGQLLSISAAHGDLETVRYLLTERLVELPTEPTDDNPAVVAAQFGHTDVVQELLESLPGPCSPQRLLNWMLALACQQGHLAVVKLLVLTYGADPESYAVRKNEFPVIVRLPLYAAIKSGNEDIAIFLIRHGAFFCSYILLDSPDPSKHLLRKYFVEASALPSSCPGKMALRVKWSHLKLPWVDLDWLIDISCQITELDLSANCLASLPSVVPWGLINLRKLNLSDNHLGELPSVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLEKLFEEESATNWIGLRKLQELDVSDNKLTELPALFLHSFKSLSCLNVSRNNLKAFPDAWACPLKCCKASKNALESLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLKLQGNQLVALPPQEKWTCRQLKTLDLSRNHFGKNEDVLKTKRISFFTTRGRQRSGTEAACVLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKSLSELYLGNNPSLRELPPELGQLGNLWQLDIEDLNIINVPAEIRKEGPKAMLSYLRAQLRKAEKCKQMKMIIVGPPRQGKSTLLEILQTGRAPQVVHSEATIRTTKWELQRPAGSRAKVESVEFTVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVASLQFWLLNIEAKAPNAVVLVVGTHLDLIETKFRVERIATLRAYVLALCRSPSGSRATGFPDITCKHLHELSCKSLEGQEGLRQLIFQVTCNMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEKQHRSLSDDVQYLTDRQLEQLVDQMPCSDIQDYEDLQSAISFLIETGSLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRLLLVGTGFTKQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENRKNTKSRHRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIMCQSEIRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHSNPSERSEDVQYFDMEDCVLTAIERDFISCPRHPDLLVPLQELVPELFMTDFPARLFLDNSKLEHSEDESAVLGQGGSGTVIYRARYQGQPVAVKRFQIKKFKNYANAPADTMLRHLRATDAMRNFSEFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENARDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDIKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGQHQLQISKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALAVVSQMKDPTFATFMYQLPCGKQTSFFSSQGQEYMVVFWEGKEESRNYTVVNTEKGLLEVQHMSCAGMKLSCQLKIQNSLWTATEDQKIYIYSLKGMCPLNTPQRALDTPAIVTCFLAVPIIKKNSHLVLAGLADGLVAVFPAAWGAPDYGCSYLCSHTANRAKFSIPDEDARQNPYPVTAMEVVNCGSEVWYSNGPGLLVIDCAALEIRRRLEPYAAPSVVTSVVCGSECRGEEVVWCLDDRANCLVLYHSATYQLCARYFCGDPSPLGDVFPVCPMGPPPLDSPTASTREPARGSIADVSFMFSEELGTQILTHQDSLTDYCSLSSCSAASPHPAPMALSSLPCSPASSASLPFSSDCEDTSQLPKPAVGSDRSVHDLSPVDGEAFSQHLQAVKILAVKDVIWVPRRGGDIIVISLEKEAGTQRGRVIAVLKARELTPHGVLTEAAVVAKDTVVCGFENENLEWCLAVWRGWGTQEFDIFYQSYEELGRLEACVRKRR

Target ORF information:

RefSeq Version	NM_001205774.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus leucine-rich repeat kinase 1 (LRRK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205774.1

ORF Insert Sequence:

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCATACA GGCGAGGAAA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTAG AGCTACCGAC GGAGCCCACT 
GACGACAACC CCGCTGTGGT GGCAGCACAG TTTGGGCACA CTGATGTCGT TCAGGAGCTA 
CTCGAGTCTT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAACTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCTGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTACGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT GCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCAGT CGTCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAC AACAAACTGA CAGAACTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTA AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACTTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAGCTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAATCACTTT GGCAAAAATG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTTGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCGCCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAACT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCATCAAC GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACAGA CAAGGCCCTG 
TACGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCCG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAT GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGCGTGGAG AGGATCGCCA CTCTGCGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTAGC AGAGAAGCAG 
CACCGCAGCC TGAGTGACGA TGTGCAGTAC CTGACAGACC GGCAGCTAGA GCAGCTGGTG 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGACC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTCG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA TGGGGTGATC AGAGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGTACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTGC CCGTCGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCG 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG CGGTTTATAG CCCGGATGCT GATCAGCTTG 
GCTGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CGTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCCTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGACGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCTGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGACTCTTCT TGGACAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGCGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TATGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AATTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCCTGGAGCT TGCCCCACTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAGAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATT 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC GTTCCACGAG 
GGTGCCCTTG GCGTGGAGGG CACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTGTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTCCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAG 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGTGGGAA GCAGACGTCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCAG TACCTATTAT AAAAAAGAAC 
TCCCACCTGG TGCTAGCAGG CCTGGCGGAC GGCCTGGTGG CGGTGTTCCC GGCGGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCTTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCGGCGC CCTCGGTGGT CACGTCGGTG 
GTGTGCGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TGTACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCGCC CCTGGACAGC 
CCCACAGCCA GCACGAGGGA GCCCGCGAGG GGATCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACACA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCGCAC CCGGCGCCGA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGTGAGGA CACCAGCCAG 
CTGCCCAAGC CCGCTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTCG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCGCG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCATGG GGTTCTCACG 
GAGGCTGCAG TGGTGGCAAA GGACACGGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ACGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGTT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRK1 ( XM_005221724.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221781.1 Bos taurus leucine-rich repeat kinase 1 (LRRK1), transcript variant X1, mRNA.

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CloneID	OBa30787
Clone ID Related Accession (Same CDS sequence)	XM_005221724.3 , XM_015459145.1 , XM_010816886.2 , XM_005221725.3 , XM_024981744.1 , XM_005221726.4 , NM_001205774.2 , NM_001205774.1 , XM_005221726.3 , XM_024981743.1 , XM_005221725.4 , XM_005221724.4
Accession Version	XM_005221724.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104530.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221724.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCATACA GGCGAGGAAA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTAG AGCTACCGAC GGAGCCCACT 
GACGACAACC CCGCTGTGGT GGCAGCACAG TTTGGGCACA CTGATGTCGT TCAGGAGCTA 
CTCGAGTCTT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAACTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCTGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTACGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT GCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCAGT CGTCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAC AACAAACTGA CAGAACTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTA AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACTTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAGCTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAATCACTTT GGCAAAAATG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTTGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCGCCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAACT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCATCAAC GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACAGA CAAGGCCCTG 
TACGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCCG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAT GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGCGTGGAG AGGATCGCCA CTCTGCGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTAGC AGAGAAGCAG 
CACCGCAGCC TGAGTGACGA TGTGCAGTAC CTGACAGACC GGCAGCTAGA GCAGCTGGTG 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGACC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTCG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA TGGGGTGATC AGAGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGTACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTGC CCGTCGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCG 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG CGGTTTATAG CCCGGATGCT GATCAGCTTG 
GCTGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CGTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCCTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGACGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCTGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGACTCTTCT TGGACAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGCGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TATGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AATTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCCTGGAGCT TGCCCCACTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAGAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATT 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC GTTCCACGAG 
GGTGCCCTTG GCGTGGAGGG CACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTGTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTCCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAG 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGTGGGAA GCAGACGTCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCAG TACCTATTAT AAAAAAGAAC 
TCCCACCTGG TGCTAGCAGG CCTGGCGGAC GGCCTGGTGG CGGTGTTCCC GGCGGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCTTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCGGCGC CCTCGGTGGT CACGTCGGTG 
GTGTGCGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TGTACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCGCC CCTGGACAGC 
CCCACAGCCA GCACGAGGGA GCCCGCGAGG GGATCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACACA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCGCAC CCGGCGCCGA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGTGAGGA CACCAGCCAG 
CTGCCCAAGC CCGCTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTCG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCGCG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCATGG GGTTCTCACG 
GAGGCTGCAG TGGTGGCAAA GGACACGGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ACGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGTT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221781.1
CDS	214..6261
Translation	MAGMSRLPPSMYWCVGPAGSAVCPGRAMETQENGAGDMGSKLSMPGSEPMVQSPRTEGIHAAYRRGNISRAQHLLREACDESTSQLEKGQLLSISAAHGDLETVRYLLTERLVELPTEPTDDNPAVVAAQFGHTDVVQELLESLPGPCSPQRLLNWMLALACQQGHLAVVKLLVLTYGADPESYAVRKNEFPVIVRLPLYAAIKSGNEDIAIFLIRHGAFFCSYILLDSPDPSKHLLRKYFVEASALPSSCPGKMALRVKWSHLKLPWVDLDWLIDISCQITELDLSANCLASLPSVVPWGLINLRKLNLSDNHLGELPSVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLEKLFEEESATNWIGLRKLQELDVSDNKLTELPALFLHSFKSLSCLNVSRNNLKAFPDAWACPLKCCKASKNALESLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLKLQGNQLVALPPQEKWTCRQLKTLDLSRNHFGKNEDVLKTKRISFFTTRGRQRSGTEAACVLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKSLSELYLGNNPSLRELPPELGQLGNLWQLDIEDLNIINVPAEIRKEGPKAMLSYLRAQLRKAEKCKQMKMIIVGPPRQGKSTLLEILQTGRAPQVVHSEATIRTTKWELQRPAGSRAKVESVEFTVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVASLQFWLLNIEAKAPNAVVLVVGTHLDLIETKFRVERIATLRAYVLALCRSPSGSRATGFPDITCKHLHELSCKSLEGQEGLRQLIFQVTCNMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEKQHRSLSDDVQYLTDRQLEQLVDQMPCSDIQDYEDLQSAISFLIETGSLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRLLLVGTGFTKQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENRKNTKSRHRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIMCQSEIRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHSNPSERSEDVQYFDMEDCVLTAIERDFISCPRHPDLLVPLQELVPELFMTDFPARLFLDNSKLEHSEDESAVLGQGGSGTVIYRARYQGQPVAVKRFQIKKFKNYANAPADTMLRHLRATDAMRNFSEFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENARDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDIKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGQHQLQISKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALAVVSQMKDPTFATFMYQLPCGKQTSFFSSQGQEYMVVFWEGKEESRNYTVVNTEKGLLEVQHMSCAGMKLSCQLKIQNSLWTATEDQKIYIYSLKGMCPLNTPQRALDTPAIVTCFLAVPIIKKNSHLVLAGLADGLVAVFPAAWGAPDYGCSYLCSHTANRAKFSIPDEDARQNPYPVTAMEVVNCGSEVWYSNGPGLLVIDCAALEIRRRLEPYAAPSVVTSVVCGSECRGEEVVWCLDDRANCLVLYHSATYQLCARYFCGDPSPLGDVFPVCPMGPPPLDSPTASTREPARGSIADVSFMFSEELGTQILTHQDSLTDYCSLSSCSAASPHPAPMALSSLPCSPASSASLPFSSDCEDTSQLPKPAVGSDRSVHDLSPVDGEAFSQHLQAVKILAVKDVIWVPRRGGDIIVISLEKEAGTQRGRVIAVLKARELTPHGVLTEAAVVAKDTVVCGFENENLEWCLAVWRGWGTQEFDIFYQSYEELGRLEACVRKRR

Target ORF information:

RefSeq Version	XM_005221724.3
Organism	Bos taurus(cattle)
Definition	Bos taurus leucine-rich repeat kinase 1 (LRRK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221724.3

ORF Insert Sequence:

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCATACA GGCGAGGAAA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTAG AGCTACCGAC GGAGCCCACT 
GACGACAACC CCGCTGTGGT GGCAGCACAG TTTGGGCACA CTGATGTCGT TCAGGAGCTA 
CTCGAGTCTT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAACTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCTGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTACGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT GCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCAGT CGTCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAC AACAAACTGA CAGAACTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTA AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACTTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAGCTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAATCACTTT GGCAAAAATG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTTGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCGCCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAACT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCATCAAC GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACAGA CAAGGCCCTG 
TACGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCCG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAT GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGCGTGGAG AGGATCGCCA CTCTGCGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTAGC AGAGAAGCAG 
CACCGCAGCC TGAGTGACGA TGTGCAGTAC CTGACAGACC GGCAGCTAGA GCAGCTGGTG 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGACC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTCG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA TGGGGTGATC AGAGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGTACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTGC CCGTCGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCG 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG CGGTTTATAG CCCGGATGCT GATCAGCTTG 
GCTGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CGTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCCTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGACGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCTGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGACTCTTCT TGGACAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGCGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TATGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AATTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCCTGGAGCT TGCCCCACTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAGAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATT 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC GTTCCACGAG 
GGTGCCCTTG GCGTGGAGGG CACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTGTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTCCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAG 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGTGGGAA GCAGACGTCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCAG TACCTATTAT AAAAAAGAAC 
TCCCACCTGG TGCTAGCAGG CCTGGCGGAC GGCCTGGTGG CGGTGTTCCC GGCGGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCTTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCGGCGC CCTCGGTGGT CACGTCGGTG 
GTGTGCGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TGTACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCGCC CCTGGACAGC 
CCCACAGCCA GCACGAGGGA GCCCGCGAGG GGATCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACACA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCGCAC CCGGCGCCGA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGTGAGGA CACCAGCCAG 
CTGCCCAAGC CCGCTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTCG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCGCG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCATGG GGTTCTCACG 
GAGGCTGCAG TGGTGGCAAA GGACACGGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ACGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGTT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRK1 ( XM_015459145.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314631.1 Bos taurus leucine-rich repeat kinase 1 (LRRK1), transcript variant X2, mRNA.

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CloneID	OBa30787
Clone ID Related Accession (Same CDS sequence)	XM_005221724.3 , XM_015459145.1 , XM_010816886.2 , XM_005221725.3 , XM_024981744.1 , XM_005221726.4 , NM_001205774.2 , NM_001205774.1 , XM_005221726.3 , XM_024981743.1 , XM_005221725.4 , XM_005221724.4
Accession Version	XM_015459145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104530.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCATACA GGCGAGGAAA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTAG AGCTACCGAC GGAGCCCACT 
GACGACAACC CCGCTGTGGT GGCAGCACAG TTTGGGCACA CTGATGTCGT TCAGGAGCTA 
CTCGAGTCTT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAACTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCTGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTACGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT GCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCAGT CGTCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAC AACAAACTGA CAGAACTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTA AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACTTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAGCTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAATCACTTT GGCAAAAATG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTTGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCGCCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAACT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCATCAAC GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACAGA CAAGGCCCTG 
TACGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCCG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAT GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGCGTGGAG AGGATCGCCA CTCTGCGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTAGC AGAGAAGCAG 
CACCGCAGCC TGAGTGACGA TGTGCAGTAC CTGACAGACC GGCAGCTAGA GCAGCTGGTG 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGACC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTCG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA TGGGGTGATC AGAGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGTACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTGC CCGTCGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCG 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG CGGTTTATAG CCCGGATGCT GATCAGCTTG 
GCTGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CGTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCCTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGACGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCTGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGACTCTTCT TGGACAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGCGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TATGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AATTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCCTGGAGCT TGCCCCACTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAGAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATT 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC GTTCCACGAG 
GGTGCCCTTG GCGTGGAGGG CACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTGTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTCCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAG 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGTGGGAA GCAGACGTCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCAG TACCTATTAT AAAAAAGAAC 
TCCCACCTGG TGCTAGCAGG CCTGGCGGAC GGCCTGGTGG CGGTGTTCCC GGCGGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCTTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCGGCGC CCTCGGTGGT CACGTCGGTG 
GTGTGCGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TGTACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCGCC CCTGGACAGC 
CCCACAGCCA GCACGAGGGA GCCCGCGAGG GGATCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACACA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCGCAC CCGGCGCCGA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGTGAGGA CACCAGCCAG 
CTGCCCAAGC CCGCTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTCG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCGCG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCATGG GGTTCTCACG 
GAGGCTGCAG TGGTGGCAAA GGACACGGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ACGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGTT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314631.1
CDS	685..6732
Translation	MAGMSRLPPSMYWCVGPAGSAVCPGRAMETQENGAGDMGSKLSMPGSEPMVQSPRTEGIHAAYRRGNISRAQHLLREACDESTSQLEKGQLLSISAAHGDLETVRYLLTERLVELPTEPTDDNPAVVAAQFGHTDVVQELLESLPGPCSPQRLLNWMLALACQQGHLAVVKLLVLTYGADPESYAVRKNEFPVIVRLPLYAAIKSGNEDIAIFLIRHGAFFCSYILLDSPDPSKHLLRKYFVEASALPSSCPGKMALRVKWSHLKLPWVDLDWLIDISCQITELDLSANCLASLPSVVPWGLINLRKLNLSDNHLGELPSVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLEKLFEEESATNWIGLRKLQELDVSDNKLTELPALFLHSFKSLSCLNVSRNNLKAFPDAWACPLKCCKASKNALESLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLKLQGNQLVALPPQEKWTCRQLKTLDLSRNHFGKNEDVLKTKRISFFTTRGRQRSGTEAACVLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKSLSELYLGNNPSLRELPPELGQLGNLWQLDIEDLNIINVPAEIRKEGPKAMLSYLRAQLRKAEKCKQMKMIIVGPPRQGKSTLLEILQTGRAPQVVHSEATIRTTKWELQRPAGSRAKVESVEFTVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVASLQFWLLNIEAKAPNAVVLVVGTHLDLIETKFRVERIATLRAYVLALCRSPSGSRATGFPDITCKHLHELSCKSLEGQEGLRQLIFQVTCNMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEKQHRSLSDDVQYLTDRQLEQLVDQMPCSDIQDYEDLQSAISFLIETGSLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRLLLVGTGFTKQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENRKNTKSRHRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIMCQSEIRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHSNPSERSEDVQYFDMEDCVLTAIERDFISCPRHPDLLVPLQELVPELFMTDFPARLFLDNSKLEHSEDESAVLGQGGSGTVIYRARYQGQPVAVKRFQIKKFKNYANAPADTMLRHLRATDAMRNFSEFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENARDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDIKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGQHQLQISKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALAVVSQMKDPTFATFMYQLPCGKQTSFFSSQGQEYMVVFWEGKEESRNYTVVNTEKGLLEVQHMSCAGMKLSCQLKIQNSLWTATEDQKIYIYSLKGMCPLNTPQRALDTPAIVTCFLAVPIIKKNSHLVLAGLADGLVAVFPAAWGAPDYGCSYLCSHTANRAKFSIPDEDARQNPYPVTAMEVVNCGSEVWYSNGPGLLVIDCAALEIRRRLEPYAAPSVVTSVVCGSECRGEEVVWCLDDRANCLVLYHSATYQLCARYFCGDPSPLGDVFPVCPMGPPPLDSPTASTREPARGSIADVSFMFSEELGTQILTHQDSLTDYCSLSSCSAASPHPAPMALSSLPCSPASSASLPFSSDCEDTSQLPKPAVGSDRSVHDLSPVDGEAFSQHLQAVKILAVKDVIWVPRRGGDIIVISLEKEAGTQRGRVIAVLKARELTPHGVLTEAAVVAKDTVVCGFENENLEWCLAVWRGWGTQEFDIFYQSYEELGRLEACVRKRR

Target ORF information:

RefSeq Version	XM_015459145.1
Organism	Bos taurus(cattle)
Definition	Bos taurus leucine-rich repeat kinase 1 (LRRK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459145.1

ORF Insert Sequence:

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCATACA GGCGAGGAAA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTAG AGCTACCGAC GGAGCCCACT 
GACGACAACC CCGCTGTGGT GGCAGCACAG TTTGGGCACA CTGATGTCGT TCAGGAGCTA 
CTCGAGTCTT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAACTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCTGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTACGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT GCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCAGT CGTCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAC AACAAACTGA CAGAACTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTA AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACTTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAGCTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAATCACTTT GGCAAAAATG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTTGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCGCCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAACT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCATCAAC GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACAGA CAAGGCCCTG 
TACGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCCG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAT GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGCGTGGAG AGGATCGCCA CTCTGCGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTAGC AGAGAAGCAG 
CACCGCAGCC TGAGTGACGA TGTGCAGTAC CTGACAGACC GGCAGCTAGA GCAGCTGGTG 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGACC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTCG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA TGGGGTGATC AGAGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGTACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTGC CCGTCGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCG 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG CGGTTTATAG CCCGGATGCT GATCAGCTTG 
GCTGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CGTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCCTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGACGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCTGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGACTCTTCT TGGACAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGCGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TATGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AATTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCCTGGAGCT TGCCCCACTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAGAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATT 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC GTTCCACGAG 
GGTGCCCTTG GCGTGGAGGG CACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTGTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTCCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAG 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGTGGGAA GCAGACGTCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCAG TACCTATTAT AAAAAAGAAC 
TCCCACCTGG TGCTAGCAGG CCTGGCGGAC GGCCTGGTGG CGGTGTTCCC GGCGGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCTTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCGGCGC CCTCGGTGGT CACGTCGGTG 
GTGTGCGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TGTACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCGCC CCTGGACAGC 
CCCACAGCCA GCACGAGGGA GCCCGCGAGG GGATCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACACA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCGCAC CCGGCGCCGA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGTGAGGA CACCAGCCAG 
CTGCCCAAGC CCGCTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTCG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCGCG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCATGG GGTTCTCACG 
GAGGCTGCAG TGGTGGCAAA GGACACGGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ACGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGTT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRK1 ( XM_010816886.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815188.1 Bos taurus leucine-rich repeat kinase 1 (LRRK1), transcript variant X3, mRNA.

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CloneID	OBa30787
Clone ID Related Accession (Same CDS sequence)	XM_005221724.3 , XM_015459145.1 , XM_010816886.2 , XM_005221725.3 , XM_024981744.1 , XM_005221726.4 , NM_001205774.2 , NM_001205774.1 , XM_005221726.3 , XM_024981743.1 , XM_005221725.4 , XM_005221724.4
Accession Version	XM_010816886.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104530.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816886.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCATACA GGCGAGGAAA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTAG AGCTACCGAC GGAGCCCACT 
GACGACAACC CCGCTGTGGT GGCAGCACAG TTTGGGCACA CTGATGTCGT TCAGGAGCTA 
CTCGAGTCTT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAACTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCTGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTACGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT GCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCAGT CGTCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAC AACAAACTGA CAGAACTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTA AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACTTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAGCTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAATCACTTT GGCAAAAATG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTTGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCGCCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAACT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCATCAAC GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACAGA CAAGGCCCTG 
TACGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCCG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAT GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGCGTGGAG AGGATCGCCA CTCTGCGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTAGC AGAGAAGCAG 
CACCGCAGCC TGAGTGACGA TGTGCAGTAC CTGACAGACC GGCAGCTAGA GCAGCTGGTG 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGACC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTCG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA TGGGGTGATC AGAGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGTACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTGC CCGTCGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCG 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG CGGTTTATAG CCCGGATGCT GATCAGCTTG 
GCTGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CGTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCCTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGACGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCTGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGACTCTTCT TGGACAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGCGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TATGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AATTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCCTGGAGCT TGCCCCACTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAGAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATT 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC GTTCCACGAG 
GGTGCCCTTG GCGTGGAGGG CACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTGTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTCCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAG 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGTGGGAA GCAGACGTCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCAG TACCTATTAT AAAAAAGAAC 
TCCCACCTGG TGCTAGCAGG CCTGGCGGAC GGCCTGGTGG CGGTGTTCCC GGCGGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCTTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCGGCGC CCTCGGTGGT CACGTCGGTG 
GTGTGCGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TGTACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCGCC CCTGGACAGC 
CCCACAGCCA GCACGAGGGA GCCCGCGAGG GGATCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACACA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCGCAC CCGGCGCCGA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGTGAGGA CACCAGCCAG 
CTGCCCAAGC CCGCTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTCG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCGCG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCATGG GGTTCTCACG 
GAGGCTGCAG TGGTGGCAAA GGACACGGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ACGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGTT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815188.1
CDS	620..6667
Translation	MAGMSRLPPSMYWCVGPAGSAVCPGRAMETQENGAGDMGSKLSMPGSEPMVQSPRTEGIHAAYRRGNISRAQHLLREACDESTSQLEKGQLLSISAAHGDLETVRYLLTERLVELPTEPTDDNPAVVAAQFGHTDVVQELLESLPGPCSPQRLLNWMLALACQQGHLAVVKLLVLTYGADPESYAVRKNEFPVIVRLPLYAAIKSGNEDIAIFLIRHGAFFCSYILLDSPDPSKHLLRKYFVEASALPSSCPGKMALRVKWSHLKLPWVDLDWLIDISCQITELDLSANCLASLPSVVPWGLINLRKLNLSDNHLGELPSVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLEKLFEEESATNWIGLRKLQELDVSDNKLTELPALFLHSFKSLSCLNVSRNNLKAFPDAWACPLKCCKASKNALESLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLKLQGNQLVALPPQEKWTCRQLKTLDLSRNHFGKNEDVLKTKRISFFTTRGRQRSGTEAACVLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKSLSELYLGNNPSLRELPPELGQLGNLWQLDIEDLNIINVPAEIRKEGPKAMLSYLRAQLRKAEKCKQMKMIIVGPPRQGKSTLLEILQTGRAPQVVHSEATIRTTKWELQRPAGSRAKVESVEFTVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVASLQFWLLNIEAKAPNAVVLVVGTHLDLIETKFRVERIATLRAYVLALCRSPSGSRATGFPDITCKHLHELSCKSLEGQEGLRQLIFQVTCNMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEKQHRSLSDDVQYLTDRQLEQLVDQMPCSDIQDYEDLQSAISFLIETGSLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRLLLVGTGFTKQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENRKNTKSRHRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIMCQSEIRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHSNPSERSEDVQYFDMEDCVLTAIERDFISCPRHPDLLVPLQELVPELFMTDFPARLFLDNSKLEHSEDESAVLGQGGSGTVIYRARYQGQPVAVKRFQIKKFKNYANAPADTMLRHLRATDAMRNFSEFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENARDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDIKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGQHQLQISKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALAVVSQMKDPTFATFMYQLPCGKQTSFFSSQGQEYMVVFWEGKEESRNYTVVNTEKGLLEVQHMSCAGMKLSCQLKIQNSLWTATEDQKIYIYSLKGMCPLNTPQRALDTPAIVTCFLAVPIIKKNSHLVLAGLADGLVAVFPAAWGAPDYGCSYLCSHTANRAKFSIPDEDARQNPYPVTAMEVVNCGSEVWYSNGPGLLVIDCAALEIRRRLEPYAAPSVVTSVVCGSECRGEEVVWCLDDRANCLVLYHSATYQLCARYFCGDPSPLGDVFPVCPMGPPPLDSPTASTREPARGSIADVSFMFSEELGTQILTHQDSLTDYCSLSSCSAASPHPAPMALSSLPCSPASSASLPFSSDCEDTSQLPKPAVGSDRSVHDLSPVDGEAFSQHLQAVKILAVKDVIWVPRRGGDIIVISLEKEAGTQRGRVIAVLKARELTPHGVLTEAAVVAKDTVVCGFENENLEWCLAVWRGWGTQEFDIFYQSYEELGRLEACVRKRR

Target ORF information:

RefSeq Version	XM_010816886.2
Organism	Bos taurus(cattle)
Definition	Bos taurus leucine-rich repeat kinase 1 (LRRK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816886.2

ORF Insert Sequence:

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCATACA GGCGAGGAAA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTAG AGCTACCGAC GGAGCCCACT 
GACGACAACC CCGCTGTGGT GGCAGCACAG TTTGGGCACA CTGATGTCGT TCAGGAGCTA 
CTCGAGTCTT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAACTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCTGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTACGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT GCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCAGT CGTCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAC AACAAACTGA CAGAACTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTA AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACTTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAGCTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAATCACTTT GGCAAAAATG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTTGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCGCCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAACT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCATCAAC GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACAGA CAAGGCCCTG 
TACGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCCG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAT GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGCGTGGAG AGGATCGCCA CTCTGCGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTAGC AGAGAAGCAG 
CACCGCAGCC TGAGTGACGA TGTGCAGTAC CTGACAGACC GGCAGCTAGA GCAGCTGGTG 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGACC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTCG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA TGGGGTGATC AGAGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGTACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTGC CCGTCGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCG 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG CGGTTTATAG CCCGGATGCT GATCAGCTTG 
GCTGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CGTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCCTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGACGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCTGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGACTCTTCT TGGACAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGCGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TATGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AATTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCCTGGAGCT TGCCCCACTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAGAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATT 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC GTTCCACGAG 
GGTGCCCTTG GCGTGGAGGG CACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTGTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTCCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAG 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGTGGGAA GCAGACGTCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCAG TACCTATTAT AAAAAAGAAC 
TCCCACCTGG TGCTAGCAGG CCTGGCGGAC GGCCTGGTGG CGGTGTTCCC GGCGGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCTTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCGGCGC CCTCGGTGGT CACGTCGGTG 
GTGTGCGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TGTACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCGCC CCTGGACAGC 
CCCACAGCCA GCACGAGGGA GCCCGCGAGG GGATCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACACA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCGCAC CCGGCGCCGA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGTGAGGA CACCAGCCAG 
CTGCCCAAGC CCGCTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTCG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCGCG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCATGG GGTTCTCACG 
GAGGCTGCAG TGGTGGCAAA GGACACGGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ACGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGTT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRK1 ( XM_005221725.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221782.1 Bos taurus leucine-rich repeat kinase 1 (LRRK1), transcript variant X4, mRNA.

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CloneID	OBa30787
Clone ID Related Accession (Same CDS sequence)	XM_005221724.3 , XM_015459145.1 , XM_010816886.2 , XM_005221725.3 , XM_024981744.1 , XM_005221726.4 , NM_001205774.2 , NM_001205774.1 , XM_005221726.3 , XM_024981743.1 , XM_005221725.4 , XM_005221724.4
Accession Version	XM_005221725.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104530.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221725.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCATACA GGCGAGGAAA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTAG AGCTACCGAC GGAGCCCACT 
GACGACAACC CCGCTGTGGT GGCAGCACAG TTTGGGCACA CTGATGTCGT TCAGGAGCTA 
CTCGAGTCTT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAACTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCTGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTACGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT GCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCAGT CGTCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAC AACAAACTGA CAGAACTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTA AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACTTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAGCTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAATCACTTT GGCAAAAATG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTTGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCGCCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAACT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCATCAAC GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACAGA CAAGGCCCTG 
TACGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCCG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAT GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGCGTGGAG AGGATCGCCA CTCTGCGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTAGC AGAGAAGCAG 
CACCGCAGCC TGAGTGACGA TGTGCAGTAC CTGACAGACC GGCAGCTAGA GCAGCTGGTG 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGACC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTCG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA TGGGGTGATC AGAGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGTACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTGC CCGTCGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCG 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG CGGTTTATAG CCCGGATGCT GATCAGCTTG 
GCTGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CGTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCCTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGACGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCTGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGACTCTTCT TGGACAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGCGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TATGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AATTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCCTGGAGCT TGCCCCACTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAGAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATT 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC GTTCCACGAG 
GGTGCCCTTG GCGTGGAGGG CACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTGTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTCCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAG 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGTGGGAA GCAGACGTCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCAG TACCTATTAT AAAAAAGAAC 
TCCCACCTGG TGCTAGCAGG CCTGGCGGAC GGCCTGGTGG CGGTGTTCCC GGCGGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCTTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCGGCGC CCTCGGTGGT CACGTCGGTG 
GTGTGCGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TGTACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCGCC CCTGGACAGC 
CCCACAGCCA GCACGAGGGA GCCCGCGAGG GGATCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACACA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCGCAC CCGGCGCCGA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGTGAGGA CACCAGCCAG 
CTGCCCAAGC CCGCTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTCG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCGCG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCATGG GGTTCTCACG 
GAGGCTGCAG TGGTGGCAAA GGACACGGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ACGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGTT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221782.1
CDS	271..6318
Translation	MAGMSRLPPSMYWCVGPAGSAVCPGRAMETQENGAGDMGSKLSMPGSEPMVQSPRTEGIHAAYRRGNISRAQHLLREACDESTSQLEKGQLLSISAAHGDLETVRYLLTERLVELPTEPTDDNPAVVAAQFGHTDVVQELLESLPGPCSPQRLLNWMLALACQQGHLAVVKLLVLTYGADPESYAVRKNEFPVIVRLPLYAAIKSGNEDIAIFLIRHGAFFCSYILLDSPDPSKHLLRKYFVEASALPSSCPGKMALRVKWSHLKLPWVDLDWLIDISCQITELDLSANCLASLPSVVPWGLINLRKLNLSDNHLGELPSVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLEKLFEEESATNWIGLRKLQELDVSDNKLTELPALFLHSFKSLSCLNVSRNNLKAFPDAWACPLKCCKASKNALESLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLKLQGNQLVALPPQEKWTCRQLKTLDLSRNHFGKNEDVLKTKRISFFTTRGRQRSGTEAACVLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKSLSELYLGNNPSLRELPPELGQLGNLWQLDIEDLNIINVPAEIRKEGPKAMLSYLRAQLRKAEKCKQMKMIIVGPPRQGKSTLLEILQTGRAPQVVHSEATIRTTKWELQRPAGSRAKVESVEFTVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVASLQFWLLNIEAKAPNAVVLVVGTHLDLIETKFRVERIATLRAYVLALCRSPSGSRATGFPDITCKHLHELSCKSLEGQEGLRQLIFQVTCNMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEKQHRSLSDDVQYLTDRQLEQLVDQMPCSDIQDYEDLQSAISFLIETGSLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRLLLVGTGFTKQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENRKNTKSRHRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIMCQSEIRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHSNPSERSEDVQYFDMEDCVLTAIERDFISCPRHPDLLVPLQELVPELFMTDFPARLFLDNSKLEHSEDESAVLGQGGSGTVIYRARYQGQPVAVKRFQIKKFKNYANAPADTMLRHLRATDAMRNFSEFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENARDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDIKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGQHQLQISKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALAVVSQMKDPTFATFMYQLPCGKQTSFFSSQGQEYMVVFWEGKEESRNYTVVNTEKGLLEVQHMSCAGMKLSCQLKIQNSLWTATEDQKIYIYSLKGMCPLNTPQRALDTPAIVTCFLAVPIIKKNSHLVLAGLADGLVAVFPAAWGAPDYGCSYLCSHTANRAKFSIPDEDARQNPYPVTAMEVVNCGSEVWYSNGPGLLVIDCAALEIRRRLEPYAAPSVVTSVVCGSECRGEEVVWCLDDRANCLVLYHSATYQLCARYFCGDPSPLGDVFPVCPMGPPPLDSPTASTREPARGSIADVSFMFSEELGTQILTHQDSLTDYCSLSSCSAASPHPAPMALSSLPCSPASSASLPFSSDCEDTSQLPKPAVGSDRSVHDLSPVDGEAFSQHLQAVKILAVKDVIWVPRRGGDIIVISLEKEAGTQRGRVIAVLKARELTPHGVLTEAAVVAKDTVVCGFENENLEWCLAVWRGWGTQEFDIFYQSYEELGRLEACVRKRR

Target ORF information:

RefSeq Version	XM_005221725.3
Organism	Bos taurus(cattle)
Definition	Bos taurus leucine-rich repeat kinase 1 (LRRK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221725.3

ORF Insert Sequence:

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCATACA GGCGAGGAAA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTAG AGCTACCGAC GGAGCCCACT 
GACGACAACC CCGCTGTGGT GGCAGCACAG TTTGGGCACA CTGATGTCGT TCAGGAGCTA 
CTCGAGTCTT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAACTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCTGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTACGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT GCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCAGT CGTCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAC AACAAACTGA CAGAACTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTA AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACTTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAGCTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAATCACTTT GGCAAAAATG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTTGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCGCCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAACT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCATCAAC GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACAGA CAAGGCCCTG 
TACGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCCG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAT GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGCGTGGAG AGGATCGCCA CTCTGCGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTAGC AGAGAAGCAG 
CACCGCAGCC TGAGTGACGA TGTGCAGTAC CTGACAGACC GGCAGCTAGA GCAGCTGGTG 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGACC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTCG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA TGGGGTGATC AGAGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGTACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTGC CCGTCGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCG 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG CGGTTTATAG CCCGGATGCT GATCAGCTTG 
GCTGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CGTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCCTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGACGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCTGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGACTCTTCT TGGACAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGCGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TATGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AATTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCCTGGAGCT TGCCCCACTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAGAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATT 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC GTTCCACGAG 
GGTGCCCTTG GCGTGGAGGG CACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTGTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTCCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAG 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGTGGGAA GCAGACGTCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCAG TACCTATTAT AAAAAAGAAC 
TCCCACCTGG TGCTAGCAGG CCTGGCGGAC GGCCTGGTGG CGGTGTTCCC GGCGGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCTTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCGGCGC CCTCGGTGGT CACGTCGGTG 
GTGTGCGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TGTACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCGCC CCTGGACAGC 
CCCACAGCCA GCACGAGGGA GCCCGCGAGG GGATCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACACA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCGCAC CCGGCGCCGA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGTGAGGA CACCAGCCAG 
CTGCCCAAGC CCGCTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTCG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCGCG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCATGG GGTTCTCACG 
GAGGCTGCAG TGGTGGCAAA GGACACGGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ACGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGTT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRK1 ( XM_024981744.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837512.1 Bos taurus leucine rich repeat kinase 1 (LRRK1), transcript variant X4, mRNA.

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CloneID	OBa30787
Clone ID Related Accession (Same CDS sequence)	XM_005221724.3 , XM_015459145.1 , XM_010816886.2 , XM_005221725.3 , XM_024981744.1 , XM_005221726.4 , NM_001205774.2 , NM_001205774.1 , XM_005221726.3 , XM_024981743.1 , XM_005221725.4 , XM_005221724.4
Accession Version	XM_024981744.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCATACA GGCGAGGAAA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTAG AGCTACCGAC GGAGCCCACT 
GACGACAACC CCGCTGTGGT GGCAGCACAG TTTGGGCACA CTGATGTCGT TCAGGAGCTA 
CTCGAGTCTT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAACTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCTGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTACGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT GCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCAGT CGTCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAC AACAAACTGA CAGAACTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTA AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACTTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAGCTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAATCACTTT GGCAAAAATG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTTGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCGCCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAACT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCATCAAC GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACAGA CAAGGCCCTG 
TACGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCCG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAT GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGCGTGGAG AGGATCGCCA CTCTGCGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTAGC AGAGAAGCAG 
CACCGCAGCC TGAGTGACGA TGTGCAGTAC CTGACAGACC GGCAGCTAGA GCAGCTGGTG 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGACC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTCG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA TGGGGTGATC AGAGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGTACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTGC CCGTCGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCG 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG CGGTTTATAG CCCGGATGCT GATCAGCTTG 
GCTGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CGTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCCTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGACGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCTGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGACTCTTCT TGGACAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGCGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TATGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AATTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCCTGGAGCT TGCCCCACTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAGAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATT 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC GTTCCACGAG 
GGTGCCCTTG GCGTGGAGGG CACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTGTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTCCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAG 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGTGGGAA GCAGACGTCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCAG TACCTATTAT AAAAAAGAAC 
TCCCACCTGG TGCTAGCAGG CCTGGCGGAC GGCCTGGTGG CGGTGTTCCC GGCGGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCTTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCGGCGC CCTCGGTGGT CACGTCGGTG 
GTGTGCGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TGTACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCGCC CCTGGACAGC 
CCCACAGCCA GCACGAGGGA GCCCGCGAGG GGATCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACACA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCGCAC CCGGCGCCGA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGTGAGGA CACCAGCCAG 
CTGCCCAAGC CCGCTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTCG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCGCG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCATGG GGTTCTCACG 
GAGGCTGCAG TGGTGGCAAA GGACACGGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ACGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGTT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837512.1
CDS	172..6219
Translation	MAGMSRLPPSMYWCVGPAGSAVCPGRAMETQENGAGDMGSKLSMPGSEPMVQSPRTEGIHAAYRRGNISRAQHLLREACDESTSQLEKGQLLSISAAHGDLETVRYLLTERLVELPTEPTDDNPAVVAAQFGHTDVVQELLESLPGPCSPQRLLNWMLALACQQGHLAVVKLLVLTYGADPESYAVRKNEFPVIVRLPLYAAIKSGNEDIAIFLIRHGAFFCSYILLDSPDPSKHLLRKYFVEASALPSSCPGKMALRVKWSHLKLPWVDLDWLIDISCQITELDLSANCLASLPSVVPWGLINLRKLNLSDNHLGELPSVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLEKLFEEESATNWIGLRKLQELDVSDNKLTELPALFLHSFKSLSCLNVSRNNLKAFPDAWACPLKCCKASKNALESLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLKLQGNQLVALPPQEKWTCRQLKTLDLSRNHFGKNEDVLKTKRISFFTTRGRQRSGTEAACVLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKSLSELYLGNNPSLRELPPELGQLGNLWQLDIEDLNIINVPAEIRKEGPKAMLSYLRAQLRKAEKCKQMKMIIVGPPRQGKSTLLEILQTGRAPQVVHSEATIRTTKWELQRPAGSRAKVESVEFTVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVASLQFWLLNIEAKAPNAVVLVVGTHLDLIETKFRVERIATLRAYVLALCRSPSGSRATGFPDITCKHLHELSCKSLEGQEGLRQLIFQVTCNMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEKQHRSLSDDVQYLTDRQLEQLVDQMPCSDIQDYEDLQSAISFLIETGSLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRLLLVGTGFTKQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENRKNTKSRHRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIMCQSEIRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHSNPSERSEDVQYFDMEDCVLTAIERDFISCPRHPDLLVPLQELVPELFMTDFPARLFLDNSKLEHSEDESAVLGQGGSGTVIYRARYQGQPVAVKRFQIKKFKNYANAPADTMLRHLRATDAMRNFSEFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENARDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDIKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGQHQLQISKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALAVVSQMKDPTFATFMYQLPCGKQTSFFSSQGQEYMVVFWEGKEESRNYTVVNTEKGLLEVQHMSCAGMKLSCQLKIQNSLWTATEDQKIYIYSLKGMCPLNTPQRALDTPAIVTCFLAVPIIKKNSHLVLAGLADGLVAVFPAAWGAPDYGCSYLCSHTANRAKFSIPDEDARQNPYPVTAMEVVNCGSEVWYSNGPGLLVIDCAALEIRRRLEPYAAPSVVTSVVCGSECRGEEVVWCLDDRANCLVLYHSATYQLCARYFCGDPSPLGDVFPVCPMGPPPLDSPTASTREPARGSIADVSFMFSEELGTQILTHQDSLTDYCSLSSCSAASPHPAPMALSSLPCSPASSASLPFSSDCEDTSQLPKPAVGSDRSVHDLSPVDGEAFSQHLQAVKILAVKDVIWVPRRGGDIIVISLEKEAGTQRGRVIAVLKARELTPHGVLTEAAVVAKDTVVCGFENENLEWCLAVWRGWGTQEFDIFYQSYEELGRLEACVRKRR

Target ORF information:

RefSeq Version	XM_024981744.1
Organism	Bos taurus(cattle)
Definition	Bos taurus leucine rich repeat kinase 1 (LRRK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981744.1

ORF Insert Sequence:

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCATACA GGCGAGGAAA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTAG AGCTACCGAC GGAGCCCACT 
GACGACAACC CCGCTGTGGT GGCAGCACAG TTTGGGCACA CTGATGTCGT TCAGGAGCTA 
CTCGAGTCTT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAACTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCTGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTACGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT GCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCAGT CGTCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAC AACAAACTGA CAGAACTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTA AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACTTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAGCTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAATCACTTT GGCAAAAATG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTTGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCGCCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAACT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCATCAAC GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACAGA CAAGGCCCTG 
TACGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCCG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAT GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGCGTGGAG AGGATCGCCA CTCTGCGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTAGC AGAGAAGCAG 
CACCGCAGCC TGAGTGACGA TGTGCAGTAC CTGACAGACC GGCAGCTAGA GCAGCTGGTG 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGACC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTCG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA TGGGGTGATC AGAGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGTACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTGC CCGTCGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCG 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG CGGTTTATAG CCCGGATGCT GATCAGCTTG 
GCTGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CGTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCCTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGACGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCTGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGACTCTTCT TGGACAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGCGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TATGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AATTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCCTGGAGCT TGCCCCACTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAGAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATT 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC GTTCCACGAG 
GGTGCCCTTG GCGTGGAGGG CACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTGTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTCCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAG 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGTGGGAA GCAGACGTCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCAG TACCTATTAT AAAAAAGAAC 
TCCCACCTGG TGCTAGCAGG CCTGGCGGAC GGCCTGGTGG CGGTGTTCCC GGCGGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCTTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCGGCGC CCTCGGTGGT CACGTCGGTG 
GTGTGCGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TGTACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCGCC CCTGGACAGC 
CCCACAGCCA GCACGAGGGA GCCCGCGAGG GGATCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACACA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCGCAC CCGGCGCCGA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGTGAGGA CACCAGCCAG 
CTGCCCAAGC CCGCTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTCG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCGCG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCATGG GGTTCTCACG 
GAGGCTGCAG TGGTGGCAAA GGACACGGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ACGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGTT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRK1 ( XM_005221726.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221783.1 Bos taurus leucine rich repeat kinase 1 (LRRK1), transcript variant X2, mRNA.

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CloneID	OBa30787
Clone ID Related Accession (Same CDS sequence)	XM_005221724.3 , XM_015459145.1 , XM_010816886.2 , XM_005221725.3 , XM_024981744.1 , XM_005221726.4 , NM_001205774.2 , NM_001205774.1 , XM_005221726.3 , XM_024981743.1 , XM_005221725.4 , XM_005221724.4
Accession Version	XM_005221726.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221726.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCATACA GGCGAGGAAA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTAG AGCTACCGAC GGAGCCCACT 
GACGACAACC CCGCTGTGGT GGCAGCACAG TTTGGGCACA CTGATGTCGT TCAGGAGCTA 
CTCGAGTCTT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAACTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCTGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTACGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT GCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCAGT CGTCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAC AACAAACTGA CAGAACTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTA AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACTTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAGCTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAATCACTTT GGCAAAAATG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTTGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCGCCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAACT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCATCAAC GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACAGA CAAGGCCCTG 
TACGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCCG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAT GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGCGTGGAG AGGATCGCCA CTCTGCGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTAGC AGAGAAGCAG 
CACCGCAGCC TGAGTGACGA TGTGCAGTAC CTGACAGACC GGCAGCTAGA GCAGCTGGTG 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGACC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTCG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA TGGGGTGATC AGAGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGTACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTGC CCGTCGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCG 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG CGGTTTATAG CCCGGATGCT GATCAGCTTG 
GCTGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CGTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCCTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGACGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCTGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGACTCTTCT TGGACAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGCGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TATGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AATTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCCTGGAGCT TGCCCCACTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAGAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATT 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC GTTCCACGAG 
GGTGCCCTTG GCGTGGAGGG CACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTGTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTCCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAG 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGTGGGAA GCAGACGTCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCAG TACCTATTAT AAAAAAGAAC 
TCCCACCTGG TGCTAGCAGG CCTGGCGGAC GGCCTGGTGG CGGTGTTCCC GGCGGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCTTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCGGCGC CCTCGGTGGT CACGTCGGTG 
GTGTGCGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TGTACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCGCC CCTGGACAGC 
CCCACAGCCA GCACGAGGGA GCCCGCGAGG GGATCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACACA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCGCAC CCGGCGCCGA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGTGAGGA CACCAGCCAG 
CTGCCCAAGC CCGCTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTCG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCGCG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCATGG GGTTCTCACG 
GAGGCTGCAG TGGTGGCAAA GGACACGGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ACGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGTT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221783.1
CDS	301..6348
Translation	MAGMSRLPPSMYWCVGPAGSAVCPGRAMETQENGAGDMGSKLSMPGSEPMVQSPRTEGIHAAYRRGNISRAQHLLREACDESTSQLEKGQLLSISAAHGDLETVRYLLTERLVELPTEPTDDNPAVVAAQFGHTDVVQELLESLPGPCSPQRLLNWMLALACQQGHLAVVKLLVLTYGADPESYAVRKNEFPVIVRLPLYAAIKSGNEDIAIFLIRHGAFFCSYILLDSPDPSKHLLRKYFVEASALPSSCPGKMALRVKWSHLKLPWVDLDWLIDISCQITELDLSANCLASLPSVVPWGLINLRKLNLSDNHLGELPSVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLEKLFEEESATNWIGLRKLQELDVSDNKLTELPALFLHSFKSLSCLNVSRNNLKAFPDAWACPLKCCKASKNALESLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLKLQGNQLVALPPQEKWTCRQLKTLDLSRNHFGKNEDVLKTKRISFFTTRGRQRSGTEAACVLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKSLSELYLGNNPSLRELPPELGQLGNLWQLDIEDLNIINVPAEIRKEGPKAMLSYLRAQLRKAEKCKQMKMIIVGPPRQGKSTLLEILQTGRAPQVVHSEATIRTTKWELQRPAGSRAKVESVEFTVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVASLQFWLLNIEAKAPNAVVLVVGTHLDLIETKFRVERIATLRAYVLALCRSPSGSRATGFPDITCKHLHELSCKSLEGQEGLRQLIFQVTCNMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEKQHRSLSDDVQYLTDRQLEQLVDQMPCSDIQDYEDLQSAISFLIETGSLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRLLLVGTGFTKQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENRKNTKSRHRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIMCQSEIRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHSNPSERSEDVQYFDMEDCVLTAIERDFISCPRHPDLLVPLQELVPELFMTDFPARLFLDNSKLEHSEDESAVLGQGGSGTVIYRARYQGQPVAVKRFQIKKFKNYANAPADTMLRHLRATDAMRNFSEFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENARDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDIKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGQHQLQISKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALAVVSQMKDPTFATFMYQLPCGKQTSFFSSQGQEYMVVFWEGKEESRNYTVVNTEKGLLEVQHMSCAGMKLSCQLKIQNSLWTATEDQKIYIYSLKGMCPLNTPQRALDTPAIVTCFLAVPIIKKNSHLVLAGLADGLVAVFPAAWGAPDYGCSYLCSHTANRAKFSIPDEDARQNPYPVTAMEVVNCGSEVWYSNGPGLLVIDCAALEIRRRLEPYAAPSVVTSVVCGSECRGEEVVWCLDDRANCLVLYHSATYQLCARYFCGDPSPLGDVFPVCPMGPPPLDSPTASTREPARGSIADVSFMFSEELGTQILTHQDSLTDYCSLSSCSAASPHPAPMALSSLPCSPASSASLPFSSDCEDTSQLPKPAVGSDRSVHDLSPVDGEAFSQHLQAVKILAVKDVIWVPRRGGDIIVISLEKEAGTQRGRVIAVLKARELTPHGVLTEAAVVAKDTVVCGFENENLEWCLAVWRGWGTQEFDIFYQSYEELGRLEACVRKRR

Target ORF information:

RefSeq Version	XM_005221726.4
Organism	Bos taurus(cattle)
Definition	Bos taurus leucine rich repeat kinase 1 (LRRK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221726.4

ORF Insert Sequence:

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCATACA GGCGAGGAAA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTAG AGCTACCGAC GGAGCCCACT 
GACGACAACC CCGCTGTGGT GGCAGCACAG TTTGGGCACA CTGATGTCGT TCAGGAGCTA 
CTCGAGTCTT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAACTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCTGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTACGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT GCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCAGT CGTCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAC AACAAACTGA CAGAACTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTA AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACTTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAGCTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAATCACTTT GGCAAAAATG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTTGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCGCCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAACT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCATCAAC GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACAGA CAAGGCCCTG 
TACGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCCG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAT GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGCGTGGAG AGGATCGCCA CTCTGCGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTAGC AGAGAAGCAG 
CACCGCAGCC TGAGTGACGA TGTGCAGTAC CTGACAGACC GGCAGCTAGA GCAGCTGGTG 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGACC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTCG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA TGGGGTGATC AGAGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGTACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTGC CCGTCGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCG 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG CGGTTTATAG CCCGGATGCT GATCAGCTTG 
GCTGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CGTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCCTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGACGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCTGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGACTCTTCT TGGACAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGCGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TATGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AATTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCCTGGAGCT TGCCCCACTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAGAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATT 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC GTTCCACGAG 
GGTGCCCTTG GCGTGGAGGG CACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTGTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTCCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAG 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGTGGGAA GCAGACGTCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCAG TACCTATTAT AAAAAAGAAC 
TCCCACCTGG TGCTAGCAGG CCTGGCGGAC GGCCTGGTGG CGGTGTTCCC GGCGGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCTTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCGGCGC CCTCGGTGGT CACGTCGGTG 
GTGTGCGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TGTACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCGCC CCTGGACAGC 
CCCACAGCCA GCACGAGGGA GCCCGCGAGG GGATCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACACA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCGCAC CCGGCGCCGA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGTGAGGA CACCAGCCAG 
CTGCCCAAGC CCGCTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTCG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCGCG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCATGG GGTTCTCACG 
GAGGCTGCAG TGGTGGCAAA GGACACGGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ACGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGTT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRK1 ( XM_005221726.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221783.1 Bos taurus leucine-rich repeat kinase 1 (LRRK1), transcript variant X5, mRNA.

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CloneID	OBa30787
Clone ID Related Accession (Same CDS sequence)	XM_005221724.3 , XM_015459145.1 , XM_010816886.2 , XM_005221725.3 , XM_024981744.1 , XM_005221726.4 , NM_001205774.2 , NM_001205774.1 , XM_005221726.3 , XM_024981743.1 , XM_005221725.4 , XM_005221724.4
Accession Version	XM_005221726.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104530.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221726.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCATACA GGCGAGGAAA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTAG AGCTACCGAC GGAGCCCACT 
GACGACAACC CCGCTGTGGT GGCAGCACAG TTTGGGCACA CTGATGTCGT TCAGGAGCTA 
CTCGAGTCTT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAACTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCTGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTACGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT GCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCAGT CGTCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAC AACAAACTGA CAGAACTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTA AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACTTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAGCTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAATCACTTT GGCAAAAATG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTTGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCGCCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAACT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCATCAAC GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACAGA CAAGGCCCTG 
TACGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCCG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAT GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGCGTGGAG AGGATCGCCA CTCTGCGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTAGC AGAGAAGCAG 
CACCGCAGCC TGAGTGACGA TGTGCAGTAC CTGACAGACC GGCAGCTAGA GCAGCTGGTG 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGACC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTCG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA TGGGGTGATC AGAGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGTACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTGC CCGTCGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCG 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG CGGTTTATAG CCCGGATGCT GATCAGCTTG 
GCTGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CGTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCCTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGACGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCTGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGACTCTTCT TGGACAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGCGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TATGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AATTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCCTGGAGCT TGCCCCACTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAGAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATT 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC GTTCCACGAG 
GGTGCCCTTG GCGTGGAGGG CACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTGTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTCCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAG 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGTGGGAA GCAGACGTCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCAG TACCTATTAT AAAAAAGAAC 
TCCCACCTGG TGCTAGCAGG CCTGGCGGAC GGCCTGGTGG CGGTGTTCCC GGCGGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCTTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCGGCGC CCTCGGTGGT CACGTCGGTG 
GTGTGCGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TGTACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCGCC CCTGGACAGC 
CCCACAGCCA GCACGAGGGA GCCCGCGAGG GGATCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACACA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCGCAC CCGGCGCCGA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGTGAGGA CACCAGCCAG 
CTGCCCAAGC CCGCTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTCG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCGCG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCATGG GGTTCTCACG 
GAGGCTGCAG TGGTGGCAAA GGACACGGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ACGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGTT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221783.1
CDS	289..6336
Translation	MAGMSRLPPSMYWCVGPAGSAVCPGRAMETQENGAGDMGSKLSMPGSEPMVQSPRTEGIHAAYRRGNISRAQHLLREACDESTSQLEKGQLLSISAAHGDLETVRYLLTERLVELPTEPTDDNPAVVAAQFGHTDVVQELLESLPGPCSPQRLLNWMLALACQQGHLAVVKLLVLTYGADPESYAVRKNEFPVIVRLPLYAAIKSGNEDIAIFLIRHGAFFCSYILLDSPDPSKHLLRKYFVEASALPSSCPGKMALRVKWSHLKLPWVDLDWLIDISCQITELDLSANCLASLPSVVPWGLINLRKLNLSDNHLGELPSVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLEKLFEEESATNWIGLRKLQELDVSDNKLTELPALFLHSFKSLSCLNVSRNNLKAFPDAWACPLKCCKASKNALESLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLKLQGNQLVALPPQEKWTCRQLKTLDLSRNHFGKNEDVLKTKRISFFTTRGRQRSGTEAACVLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKSLSELYLGNNPSLRELPPELGQLGNLWQLDIEDLNIINVPAEIRKEGPKAMLSYLRAQLRKAEKCKQMKMIIVGPPRQGKSTLLEILQTGRAPQVVHSEATIRTTKWELQRPAGSRAKVESVEFTVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVASLQFWLLNIEAKAPNAVVLVVGTHLDLIETKFRVERIATLRAYVLALCRSPSGSRATGFPDITCKHLHELSCKSLEGQEGLRQLIFQVTCNMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEKQHRSLSDDVQYLTDRQLEQLVDQMPCSDIQDYEDLQSAISFLIETGSLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRLLLVGTGFTKQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENRKNTKSRHRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIMCQSEIRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHSNPSERSEDVQYFDMEDCVLTAIERDFISCPRHPDLLVPLQELVPELFMTDFPARLFLDNSKLEHSEDESAVLGQGGSGTVIYRARYQGQPVAVKRFQIKKFKNYANAPADTMLRHLRATDAMRNFSEFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENARDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDIKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGQHQLQISKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALAVVSQMKDPTFATFMYQLPCGKQTSFFSSQGQEYMVVFWEGKEESRNYTVVNTEKGLLEVQHMSCAGMKLSCQLKIQNSLWTATEDQKIYIYSLKGMCPLNTPQRALDTPAIVTCFLAVPIIKKNSHLVLAGLADGLVAVFPAAWGAPDYGCSYLCSHTANRAKFSIPDEDARQNPYPVTAMEVVNCGSEVWYSNGPGLLVIDCAALEIRRRLEPYAAPSVVTSVVCGSECRGEEVVWCLDDRANCLVLYHSATYQLCARYFCGDPSPLGDVFPVCPMGPPPLDSPTASTREPARGSIADVSFMFSEELGTQILTHQDSLTDYCSLSSCSAASPHPAPMALSSLPCSPASSASLPFSSDCEDTSQLPKPAVGSDRSVHDLSPVDGEAFSQHLQAVKILAVKDVIWVPRRGGDIIVISLEKEAGTQRGRVIAVLKARELTPHGVLTEAAVVAKDTVVCGFENENLEWCLAVWRGWGTQEFDIFYQSYEELGRLEACVRKRR

Target ORF information:

RefSeq Version	XM_005221726.3
Organism	Bos taurus(cattle)
Definition	Bos taurus leucine-rich repeat kinase 1 (LRRK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221726.3

ORF Insert Sequence:

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCATACA GGCGAGGAAA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTAG AGCTACCGAC GGAGCCCACT 
GACGACAACC CCGCTGTGGT GGCAGCACAG TTTGGGCACA CTGATGTCGT TCAGGAGCTA 
CTCGAGTCTT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAACTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCTGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTACGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT GCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCAGT CGTCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAC AACAAACTGA CAGAACTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTA AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACTTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAGCTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAATCACTTT GGCAAAAATG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTTGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCGCCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAACT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCATCAAC GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACAGA CAAGGCCCTG 
TACGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCCG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAT GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGCGTGGAG AGGATCGCCA CTCTGCGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTAGC AGAGAAGCAG 
CACCGCAGCC TGAGTGACGA TGTGCAGTAC CTGACAGACC GGCAGCTAGA GCAGCTGGTG 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGACC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTCG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA TGGGGTGATC AGAGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGTACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTGC CCGTCGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCG 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG CGGTTTATAG CCCGGATGCT GATCAGCTTG 
GCTGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CGTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCCTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGACGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCTGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGACTCTTCT TGGACAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGCGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TATGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AATTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCCTGGAGCT TGCCCCACTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAGAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATT 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC GTTCCACGAG 
GGTGCCCTTG GCGTGGAGGG CACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTGTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTCCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAG 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGTGGGAA GCAGACGTCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCAG TACCTATTAT AAAAAAGAAC 
TCCCACCTGG TGCTAGCAGG CCTGGCGGAC GGCCTGGTGG CGGTGTTCCC GGCGGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCTTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCGGCGC CCTCGGTGGT CACGTCGGTG 
GTGTGCGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TGTACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCGCC CCTGGACAGC 
CCCACAGCCA GCACGAGGGA GCCCGCGAGG GGATCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACACA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCGCAC CCGGCGCCGA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGTGAGGA CACCAGCCAG 
CTGCCCAAGC CCGCTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTCG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCGCG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCATGG GGTTCTCACG 
GAGGCTGCAG TGGTGGCAAA GGACACGGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ACGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGTT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRK1 ( XM_024981743.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837511.1 Bos taurus leucine rich repeat kinase 1 (LRRK1), transcript variant X1, mRNA.

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CloneID	OBa30787
Clone ID Related Accession (Same CDS sequence)	XM_005221724.3 , XM_015459145.1 , XM_010816886.2 , XM_005221725.3 , XM_024981744.1 , XM_005221726.4 , NM_001205774.2 , NM_001205774.1 , XM_005221726.3 , XM_024981743.1 , XM_005221725.4 , XM_005221724.4
Accession Version	XM_024981743.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCATACA GGCGAGGAAA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTAG AGCTACCGAC GGAGCCCACT 
GACGACAACC CCGCTGTGGT GGCAGCACAG TTTGGGCACA CTGATGTCGT TCAGGAGCTA 
CTCGAGTCTT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAACTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCTGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTACGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT GCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCAGT CGTCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAC AACAAACTGA CAGAACTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTA AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACTTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAGCTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAATCACTTT GGCAAAAATG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTTGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCGCCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAACT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCATCAAC GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACAGA CAAGGCCCTG 
TACGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCCG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAT GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGCGTGGAG AGGATCGCCA CTCTGCGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTAGC AGAGAAGCAG 
CACCGCAGCC TGAGTGACGA TGTGCAGTAC CTGACAGACC GGCAGCTAGA GCAGCTGGTG 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGACC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTCG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA TGGGGTGATC AGAGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGTACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTGC CCGTCGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCG 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG CGGTTTATAG CCCGGATGCT GATCAGCTTG 
GCTGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CGTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCCTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGACGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCTGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGACTCTTCT TGGACAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGCGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TATGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AATTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCCTGGAGCT TGCCCCACTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAGAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATT 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC GTTCCACGAG 
GGTGCCCTTG GCGTGGAGGG CACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTGTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTCCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAG 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGTGGGAA GCAGACGTCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCAG TACCTATTAT AAAAAAGAAC 
TCCCACCTGG TGCTAGCAGG CCTGGCGGAC GGCCTGGTGG CGGTGTTCCC GGCGGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCTTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCGGCGC CCTCGGTGGT CACGTCGGTG 
GTGTGCGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TGTACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCGCC CCTGGACAGC 
CCCACAGCCA GCACGAGGGA GCCCGCGAGG GGATCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACACA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCGCAC CCGGCGCCGA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGTGAGGA CACCAGCCAG 
CTGCCCAAGC CCGCTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTCG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCGCG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCATGG GGTTCTCACG 
GAGGCTGCAG TGGTGGCAAA GGACACGGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ACGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGTT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837511.1
CDS	633..6680
Translation	MAGMSRLPPSMYWCVGPAGSAVCPGRAMETQENGAGDMGSKLSMPGSEPMVQSPRTEGIHAAYRRGNISRAQHLLREACDESTSQLEKGQLLSISAAHGDLETVRYLLTERLVELPTEPTDDNPAVVAAQFGHTDVVQELLESLPGPCSPQRLLNWMLALACQQGHLAVVKLLVLTYGADPESYAVRKNEFPVIVRLPLYAAIKSGNEDIAIFLIRHGAFFCSYILLDSPDPSKHLLRKYFVEASALPSSCPGKMALRVKWSHLKLPWVDLDWLIDISCQITELDLSANCLASLPSVVPWGLINLRKLNLSDNHLGELPSVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLEKLFEEESATNWIGLRKLQELDVSDNKLTELPALFLHSFKSLSCLNVSRNNLKAFPDAWACPLKCCKASKNALESLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLKLQGNQLVALPPQEKWTCRQLKTLDLSRNHFGKNEDVLKTKRISFFTTRGRQRSGTEAACVLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKSLSELYLGNNPSLRELPPELGQLGNLWQLDIEDLNIINVPAEIRKEGPKAMLSYLRAQLRKAEKCKQMKMIIVGPPRQGKSTLLEILQTGRAPQVVHSEATIRTTKWELQRPAGSRAKVESVEFTVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVASLQFWLLNIEAKAPNAVVLVVGTHLDLIETKFRVERIATLRAYVLALCRSPSGSRATGFPDITCKHLHELSCKSLEGQEGLRQLIFQVTCNMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEKQHRSLSDDVQYLTDRQLEQLVDQMPCSDIQDYEDLQSAISFLIETGSLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRLLLVGTGFTKQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENRKNTKSRHRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIMCQSEIRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHSNPSERSEDVQYFDMEDCVLTAIERDFISCPRHPDLLVPLQELVPELFMTDFPARLFLDNSKLEHSEDESAVLGQGGSGTVIYRARYQGQPVAVKRFQIKKFKNYANAPADTMLRHLRATDAMRNFSEFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENARDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDIKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGQHQLQISKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALAVVSQMKDPTFATFMYQLPCGKQTSFFSSQGQEYMVVFWEGKEESRNYTVVNTEKGLLEVQHMSCAGMKLSCQLKIQNSLWTATEDQKIYIYSLKGMCPLNTPQRALDTPAIVTCFLAVPIIKKNSHLVLAGLADGLVAVFPAAWGAPDYGCSYLCSHTANRAKFSIPDEDARQNPYPVTAMEVVNCGSEVWYSNGPGLLVIDCAALEIRRRLEPYAAPSVVTSVVCGSECRGEEVVWCLDDRANCLVLYHSATYQLCARYFCGDPSPLGDVFPVCPMGPPPLDSPTASTREPARGSIADVSFMFSEELGTQILTHQDSLTDYCSLSSCSAASPHPAPMALSSLPCSPASSASLPFSSDCEDTSQLPKPAVGSDRSVHDLSPVDGEAFSQHLQAVKILAVKDVIWVPRRGGDIIVISLEKEAGTQRGRVIAVLKARELTPHGVLTEAAVVAKDTVVCGFENENLEWCLAVWRGWGTQEFDIFYQSYEELGRLEACVRKRR

Target ORF information:

RefSeq Version	XM_024981743.1
Organism	Bos taurus(cattle)
Definition	Bos taurus leucine rich repeat kinase 1 (LRRK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981743.1

ORF Insert Sequence:

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCATACA GGCGAGGAAA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTAG AGCTACCGAC GGAGCCCACT 
GACGACAACC CCGCTGTGGT GGCAGCACAG TTTGGGCACA CTGATGTCGT TCAGGAGCTA 
CTCGAGTCTT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAACTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCTGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTACGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT GCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCAGT CGTCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAC AACAAACTGA CAGAACTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTA AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACTTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAGCTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAATCACTTT GGCAAAAATG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTTGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCGCCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAACT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCATCAAC GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACAGA CAAGGCCCTG 
TACGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCCG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAT GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGCGTGGAG AGGATCGCCA CTCTGCGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTAGC AGAGAAGCAG 
CACCGCAGCC TGAGTGACGA TGTGCAGTAC CTGACAGACC GGCAGCTAGA GCAGCTGGTG 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGACC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTCG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA TGGGGTGATC AGAGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGTACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTGC CCGTCGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCG 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG CGGTTTATAG CCCGGATGCT GATCAGCTTG 
GCTGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CGTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCCTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGACGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCTGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGACTCTTCT TGGACAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGCGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TATGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AATTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCCTGGAGCT TGCCCCACTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAGAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATT 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC GTTCCACGAG 
GGTGCCCTTG GCGTGGAGGG CACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTGTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTCCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAG 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGTGGGAA GCAGACGTCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCAG TACCTATTAT AAAAAAGAAC 
TCCCACCTGG TGCTAGCAGG CCTGGCGGAC GGCCTGGTGG CGGTGTTCCC GGCGGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCTTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCGGCGC CCTCGGTGGT CACGTCGGTG 
GTGTGCGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TGTACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCGCC CCTGGACAGC 
CCCACAGCCA GCACGAGGGA GCCCGCGAGG GGATCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACACA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCGCAC CCGGCGCCGA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGTGAGGA CACCAGCCAG 
CTGCCCAAGC CCGCTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTCG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCGCG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCATGG GGTTCTCACG 
GAGGCTGCAG TGGTGGCAAA GGACACGGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ACGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGTT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRK1 ( XM_005221725.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221782.1 Bos taurus leucine rich repeat kinase 1 (LRRK1), transcript variant X3, mRNA.

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CloneID	OBa30787
Clone ID Related Accession (Same CDS sequence)	XM_005221724.3 , XM_015459145.1 , XM_010816886.2 , XM_005221725.3 , XM_024981744.1 , XM_005221726.4 , NM_001205774.2 , NM_001205774.1 , XM_005221726.3 , XM_024981743.1 , XM_005221725.4 , XM_005221724.4
Accession Version	XM_005221725.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221725.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCATACA GGCGAGGAAA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTAG AGCTACCGAC GGAGCCCACT 
GACGACAACC CCGCTGTGGT GGCAGCACAG TTTGGGCACA CTGATGTCGT TCAGGAGCTA 
CTCGAGTCTT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAACTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCTGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTACGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT GCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCAGT CGTCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAC AACAAACTGA CAGAACTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTA AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACTTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAGCTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAATCACTTT GGCAAAAATG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTTGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCGCCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAACT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCATCAAC GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACAGA CAAGGCCCTG 
TACGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCCG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAT GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGCGTGGAG AGGATCGCCA CTCTGCGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTAGC AGAGAAGCAG 
CACCGCAGCC TGAGTGACGA TGTGCAGTAC CTGACAGACC GGCAGCTAGA GCAGCTGGTG 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGACC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTCG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA TGGGGTGATC AGAGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGTACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTGC CCGTCGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCG 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG CGGTTTATAG CCCGGATGCT GATCAGCTTG 
GCTGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CGTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCCTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGACGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCTGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGACTCTTCT TGGACAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGCGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TATGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AATTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCCTGGAGCT TGCCCCACTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAGAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATT 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC GTTCCACGAG 
GGTGCCCTTG GCGTGGAGGG CACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTGTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTCCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAG 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGTGGGAA GCAGACGTCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCAG TACCTATTAT AAAAAAGAAC 
TCCCACCTGG TGCTAGCAGG CCTGGCGGAC GGCCTGGTGG CGGTGTTCCC GGCGGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCTTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCGGCGC CCTCGGTGGT CACGTCGGTG 
GTGTGCGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TGTACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCGCC CCTGGACAGC 
CCCACAGCCA GCACGAGGGA GCCCGCGAGG GGATCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACACA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCGCAC CCGGCGCCGA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGTGAGGA CACCAGCCAG 
CTGCCCAAGC CCGCTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTCG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCGCG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCATGG GGTTCTCACG 
GAGGCTGCAG TGGTGGCAAA GGACACGGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ACGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGTT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221782.1
CDS	279..6326
Translation	MAGMSRLPPSMYWCVGPAGSAVCPGRAMETQENGAGDMGSKLSMPGSEPMVQSPRTEGIHAAYRRGNISRAQHLLREACDESTSQLEKGQLLSISAAHGDLETVRYLLTERLVELPTEPTDDNPAVVAAQFGHTDVVQELLESLPGPCSPQRLLNWMLALACQQGHLAVVKLLVLTYGADPESYAVRKNEFPVIVRLPLYAAIKSGNEDIAIFLIRHGAFFCSYILLDSPDPSKHLLRKYFVEASALPSSCPGKMALRVKWSHLKLPWVDLDWLIDISCQITELDLSANCLASLPSVVPWGLINLRKLNLSDNHLGELPSVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLEKLFEEESATNWIGLRKLQELDVSDNKLTELPALFLHSFKSLSCLNVSRNNLKAFPDAWACPLKCCKASKNALESLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLKLQGNQLVALPPQEKWTCRQLKTLDLSRNHFGKNEDVLKTKRISFFTTRGRQRSGTEAACVLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKSLSELYLGNNPSLRELPPELGQLGNLWQLDIEDLNIINVPAEIRKEGPKAMLSYLRAQLRKAEKCKQMKMIIVGPPRQGKSTLLEILQTGRAPQVVHSEATIRTTKWELQRPAGSRAKVESVEFTVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVASLQFWLLNIEAKAPNAVVLVVGTHLDLIETKFRVERIATLRAYVLALCRSPSGSRATGFPDITCKHLHELSCKSLEGQEGLRQLIFQVTCNMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEKQHRSLSDDVQYLTDRQLEQLVDQMPCSDIQDYEDLQSAISFLIETGSLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRLLLVGTGFTKQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENRKNTKSRHRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIMCQSEIRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHSNPSERSEDVQYFDMEDCVLTAIERDFISCPRHPDLLVPLQELVPELFMTDFPARLFLDNSKLEHSEDESAVLGQGGSGTVIYRARYQGQPVAVKRFQIKKFKNYANAPADTMLRHLRATDAMRNFSEFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENARDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDIKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGQHQLQISKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALAVVSQMKDPTFATFMYQLPCGKQTSFFSSQGQEYMVVFWEGKEESRNYTVVNTEKGLLEVQHMSCAGMKLSCQLKIQNSLWTATEDQKIYIYSLKGMCPLNTPQRALDTPAIVTCFLAVPIIKKNSHLVLAGLADGLVAVFPAAWGAPDYGCSYLCSHTANRAKFSIPDEDARQNPYPVTAMEVVNCGSEVWYSNGPGLLVIDCAALEIRRRLEPYAAPSVVTSVVCGSECRGEEVVWCLDDRANCLVLYHSATYQLCARYFCGDPSPLGDVFPVCPMGPPPLDSPTASTREPARGSIADVSFMFSEELGTQILTHQDSLTDYCSLSSCSAASPHPAPMALSSLPCSPASSASLPFSSDCEDTSQLPKPAVGSDRSVHDLSPVDGEAFSQHLQAVKILAVKDVIWVPRRGGDIIVISLEKEAGTQRGRVIAVLKARELTPHGVLTEAAVVAKDTVVCGFENENLEWCLAVWRGWGTQEFDIFYQSYEELGRLEACVRKRR

Target ORF information:

RefSeq Version	XM_005221725.4
Organism	Bos taurus(cattle)
Definition	Bos taurus leucine rich repeat kinase 1 (LRRK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221725.4

ORF Insert Sequence:

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCATACA GGCGAGGAAA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTAG AGCTACCGAC GGAGCCCACT 
GACGACAACC CCGCTGTGGT GGCAGCACAG TTTGGGCACA CTGATGTCGT TCAGGAGCTA 
CTCGAGTCTT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAACTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCTGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTACGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT GCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCAGT CGTCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAC AACAAACTGA CAGAACTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTA AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACTTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAGCTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAATCACTTT GGCAAAAATG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTTGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCGCCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAACT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCATCAAC GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACAGA CAAGGCCCTG 
TACGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCCG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAT GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGCGTGGAG AGGATCGCCA CTCTGCGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTAGC AGAGAAGCAG 
CACCGCAGCC TGAGTGACGA TGTGCAGTAC CTGACAGACC GGCAGCTAGA GCAGCTGGTG 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGACC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTCG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA TGGGGTGATC AGAGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGTACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTGC CCGTCGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCG 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG CGGTTTATAG CCCGGATGCT GATCAGCTTG 
GCTGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CGTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCCTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGACGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCTGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGACTCTTCT TGGACAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGCGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TATGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AATTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCCTGGAGCT TGCCCCACTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAGAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATT 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC GTTCCACGAG 
GGTGCCCTTG GCGTGGAGGG CACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTGTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTCCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAG 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGTGGGAA GCAGACGTCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCAG TACCTATTAT AAAAAAGAAC 
TCCCACCTGG TGCTAGCAGG CCTGGCGGAC GGCCTGGTGG CGGTGTTCCC GGCGGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCTTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCGGCGC CCTCGGTGGT CACGTCGGTG 
GTGTGCGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TGTACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCGCC CCTGGACAGC 
CCCACAGCCA GCACGAGGGA GCCCGCGAGG GGATCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACACA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCGCAC CCGGCGCCGA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGTGAGGA CACCAGCCAG 
CTGCCCAAGC CCGCTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTCG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCGCG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCATGG GGTTCTCACG 
GAGGCTGCAG TGGTGGCAAA GGACACGGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ACGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGTT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRK1 ( XM_005221724.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221781.1 Bos taurus leucine rich repeat kinase 1 (LRRK1), transcript variant X5, mRNA.

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CloneID	OBa30787
Clone ID Related Accession (Same CDS sequence)	XM_005221724.3 , XM_015459145.1 , XM_010816886.2 , XM_005221725.3 , XM_024981744.1 , XM_005221726.4 , NM_001205774.2 , NM_001205774.1 , XM_005221726.3 , XM_024981743.1 , XM_005221725.4 , XM_005221724.4
Accession Version	XM_005221724.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221724.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCATACA GGCGAGGAAA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTAG AGCTACCGAC GGAGCCCACT 
GACGACAACC CCGCTGTGGT GGCAGCACAG TTTGGGCACA CTGATGTCGT TCAGGAGCTA 
CTCGAGTCTT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAACTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCTGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTACGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT GCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCAGT CGTCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAC AACAAACTGA CAGAACTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTA AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACTTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAGCTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAATCACTTT GGCAAAAATG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTTGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCGCCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAACT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCATCAAC GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACAGA CAAGGCCCTG 
TACGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCCG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAT GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGCGTGGAG AGGATCGCCA CTCTGCGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTAGC AGAGAAGCAG 
CACCGCAGCC TGAGTGACGA TGTGCAGTAC CTGACAGACC GGCAGCTAGA GCAGCTGGTG 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGACC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTCG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA TGGGGTGATC AGAGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGTACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTGC CCGTCGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCG 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG CGGTTTATAG CCCGGATGCT GATCAGCTTG 
GCTGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CGTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCCTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGACGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCTGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGACTCTTCT TGGACAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGCGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TATGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AATTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCCTGGAGCT TGCCCCACTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAGAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATT 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC GTTCCACGAG 
GGTGCCCTTG GCGTGGAGGG CACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTGTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTCCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAG 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGTGGGAA GCAGACGTCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCAG TACCTATTAT AAAAAAGAAC 
TCCCACCTGG TGCTAGCAGG CCTGGCGGAC GGCCTGGTGG CGGTGTTCCC GGCGGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCTTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCGGCGC CCTCGGTGGT CACGTCGGTG 
GTGTGCGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TGTACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCGCC CCTGGACAGC 
CCCACAGCCA GCACGAGGGA GCCCGCGAGG GGATCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACACA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCGCAC CCGGCGCCGA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGTGAGGA CACCAGCCAG 
CTGCCCAAGC CCGCTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTCG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCGCG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCATGG GGTTCTCACG 
GAGGCTGCAG TGGTGGCAAA GGACACGGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ACGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGTT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221781.1
CDS	273..6320
Translation	MAGMSRLPPSMYWCVGPAGSAVCPGRAMETQENGAGDMGSKLSMPGSEPMVQSPRTEGIHAAYRRGNISRAQHLLREACDESTSQLEKGQLLSISAAHGDLETVRYLLTERLVELPTEPTDDNPAVVAAQFGHTDVVQELLESLPGPCSPQRLLNWMLALACQQGHLAVVKLLVLTYGADPESYAVRKNEFPVIVRLPLYAAIKSGNEDIAIFLIRHGAFFCSYILLDSPDPSKHLLRKYFVEASALPSSCPGKMALRVKWSHLKLPWVDLDWLIDISCQITELDLSANCLASLPSVVPWGLINLRKLNLSDNHLGELPSVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLEKLFEEESATNWIGLRKLQELDVSDNKLTELPALFLHSFKSLSCLNVSRNNLKAFPDAWACPLKCCKASKNALESLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLKLQGNQLVALPPQEKWTCRQLKTLDLSRNHFGKNEDVLKTKRISFFTTRGRQRSGTEAACVLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKSLSELYLGNNPSLRELPPELGQLGNLWQLDIEDLNIINVPAEIRKEGPKAMLSYLRAQLRKAEKCKQMKMIIVGPPRQGKSTLLEILQTGRAPQVVHSEATIRTTKWELQRPAGSRAKVESVEFTVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVASLQFWLLNIEAKAPNAVVLVVGTHLDLIETKFRVERIATLRAYVLALCRSPSGSRATGFPDITCKHLHELSCKSLEGQEGLRQLIFQVTCNMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEKQHRSLSDDVQYLTDRQLEQLVDQMPCSDIQDYEDLQSAISFLIETGSLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRLLLVGTGFTKQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENRKNTKSRHRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIMCQSEIRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHSNPSERSEDVQYFDMEDCVLTAIERDFISCPRHPDLLVPLQELVPELFMTDFPARLFLDNSKLEHSEDESAVLGQGGSGTVIYRARYQGQPVAVKRFQIKKFKNYANAPADTMLRHLRATDAMRNFSEFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENARDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDIKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGQHQLQISKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALAVVSQMKDPTFATFMYQLPCGKQTSFFSSQGQEYMVVFWEGKEESRNYTVVNTEKGLLEVQHMSCAGMKLSCQLKIQNSLWTATEDQKIYIYSLKGMCPLNTPQRALDTPAIVTCFLAVPIIKKNSHLVLAGLADGLVAVFPAAWGAPDYGCSYLCSHTANRAKFSIPDEDARQNPYPVTAMEVVNCGSEVWYSNGPGLLVIDCAALEIRRRLEPYAAPSVVTSVVCGSECRGEEVVWCLDDRANCLVLYHSATYQLCARYFCGDPSPLGDVFPVCPMGPPPLDSPTASTREPARGSIADVSFMFSEELGTQILTHQDSLTDYCSLSSCSAASPHPAPMALSSLPCSPASSASLPFSSDCEDTSQLPKPAVGSDRSVHDLSPVDGEAFSQHLQAVKILAVKDVIWVPRRGGDIIVISLEKEAGTQRGRVIAVLKARELTPHGVLTEAAVVAKDTVVCGFENENLEWCLAVWRGWGTQEFDIFYQSYEELGRLEACVRKRR

Target ORF information:

RefSeq Version	XM_005221724.4
Organism	Bos taurus(cattle)
Definition	Bos taurus leucine rich repeat kinase 1 (LRRK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221724.4

ORF Insert Sequence:

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCATACA GGCGAGGAAA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTAG AGCTACCGAC GGAGCCCACT 
GACGACAACC CCGCTGTGGT GGCAGCACAG TTTGGGCACA CTGATGTCGT TCAGGAGCTA 
CTCGAGTCTT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAACTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCTGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTACGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT GCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCAGT CGTCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAC AACAAACTGA CAGAACTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTA AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACTTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAGCTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAATCACTTT GGCAAAAATG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTTGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCGCCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAACT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCATCAAC GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACAGA CAAGGCCCTG 
TACGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCCG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAT GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGCGTGGAG AGGATCGCCA CTCTGCGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTAGC AGAGAAGCAG 
CACCGCAGCC TGAGTGACGA TGTGCAGTAC CTGACAGACC GGCAGCTAGA GCAGCTGGTG 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGACC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTCG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA TGGGGTGATC AGAGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGTACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTGC CCGTCGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCG 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG CGGTTTATAG CCCGGATGCT GATCAGCTTG 
GCTGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CGTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCCTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGACGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCTGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGACTCTTCT TGGACAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGCGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TATGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AATTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCCTGGAGCT TGCCCCACTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAGAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATT 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC GTTCCACGAG 
GGTGCCCTTG GCGTGGAGGG CACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTGTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTCCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAG 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGTGGGAA GCAGACGTCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCAG TACCTATTAT AAAAAAGAAC 
TCCCACCTGG TGCTAGCAGG CCTGGCGGAC GGCCTGGTGG CGGTGTTCCC GGCGGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCTTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCGGCGC CCTCGGTGGT CACGTCGGTG 
GTGTGCGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TGTACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCGCC CCTGGACAGC 
CCCACAGCCA GCACGAGGGA GCCCGCGAGG GGATCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACACA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCGCAC CCGGCGCCGA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGTGAGGA CACCAGCCAG 
CTGCCCAAGC CCGCTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTCG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCGCG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCATGG GGTTCTCACG 
GAGGCTGCAG TGGTGGCAAA GGACACGGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ACGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGTT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	LRRK1
Entrez Gene ID	513994
Full Name	leucine rich repeat kinase 1
General protein information	Preferred Names leucine rich repeat kinase 1 Names leucine-rich repeat serine/threonine-protein kinase 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Danio rerio (zebrafish)	lrrk1	XP_002667476.3
Bos taurus (cattle)	LRRK1	NP_001192703.1
Xenopus tropicalis (tropical clawed frog)	lrrk1	XP_002939309.2
Homo sapiens (human)	LRRK1	NP_078928.3
Pan troglodytes (chimpanzee)	LRRK1	XP_510623.3
Rattus norvegicus (Norway rat)	Lrrk1	NP_001178553.1
	LRRK1	XP_001084079.1
Canis lupus familiaris (dog)	LRRK1	XP_005618338.1
Mus musculus (house mouse)	Lrrk1	NP_666303.3
Gallus gallus (chicken)	LRRK1	XP_003641870.2

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LRRK1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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