PIKFYVE gene cDNA ORF clone, Equus caballus(horse)

mRNA	Protein	Name
XM_014732622.1	XP_014588108.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_023622521.1	XP_023478289.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
XM_023622523.1	XP_023478291.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
XM_023622524.1	XP_023478292.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
XM_023622529.1	XP_023478297.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X10
XM_023622530.1	XP_023478298.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X11
XM_023622525.1	XP_023478293.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
XM_023622526.1	XP_023478294.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X7
XM_023622519.1	XP_023478287.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_023622527.1	XP_023478295.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X8
XM_001505123.3	XP_001505173.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
XM_023622517.1	XP_023478285.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_023622518.1	XP_023478286.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_023622528.1	XP_023478296.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X9
XM_023622532.1	XP_023478300.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X13
XM_023622520.1	XP_023478288.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_023622522.1	XP_023478290.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
XM_023622531.1	XP_023478299.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X12

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHb36394	XM_014732622.1 Latest version!	Equus caballus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OHb36394	XM_023622531.1 Latest version!	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OHb52480	XM_023622532.1 Latest version!	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHb52479	XM_023622530.1 Latest version!	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHb52469	XM_023622518.1 Latest version!	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb52469	XM_023622517.1 Latest version!	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb52469	XM_023622520.1 Latest version!	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb52469	XM_023622519.1 Latest version!	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb52470	XM_023622521.1 Latest version!	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb52471	XM_023622522.1 Latest version!	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb52472	XM_023622523.1 Latest version!	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb52473	XM_023622524.1 Latest version!	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb52474	XM_023622525.1 Latest version!	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb52475	XM_023622526.1 Latest version!	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb52476	XM_023622527.1 Latest version!	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb52477	XM_023622528.1 Latest version!	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb52478	XM_023622529.1 Latest version!	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb08541	XM_001505123.3 Latest version!	Equus caballus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

PIKFYVE ( XM_014732622.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014588108.1 Equus caballus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHb36394
Clone ID Related Accession (Same CDS sequence)	XM_014732622.1 , XM_023622531.1
Accession Version	XM_014732622.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867384.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGCAGA GAACAGTTTG CAGCACCCCC AGGAGAACAC AGACACAAGA 
AGGAAAACAG AACCTACCTT TGGCGGTCAT GACCCTCGTA CAGCTGTTCA GCTTCGAAGC 
CTCAGCACAG TGTTAAAACG CCTCAAGGAA ATCATGGAGG GGAAAAGCCA GGACAGTGAC 
CTGAAGCAGT ACTGGATGCC GGACAGCCAG TGCAGAGAGT GCTACGACTG CAGCGAGAAG 
TTCACCACCT TCCGGCGCAG GCACCACTGC CGGCTCTGCG GGCAGATTTT CTGCAGCCGC 
TGCTGCAATC AAGAGATCCC TGGGAAGTTT ATGGGCTATA CAGGGGACCT CCGAGCTTGC 
ACATACTGTA GAAAAATAGC CTTAAGTTAT GCTCATTCCA CAGACAGTAA TTCCATTGGG 
GAAGACTTGA ATGCTCTTTC AGATTCTGCT TCCTCTGTGT CTGTACTTGA TCCGAGTGAA 
CCCCGAACAC CTGTTGGGAG TAGGAAAGCC AGCCGGAACA TCTTTTTAGA GGATGATTTT 
GCCTGGCAAA GTTTGATTCA TCCAGACTCC TCAAATACTG CTCTTTCAAC AAGACTTGTA 
TCTGTTCAAG AGGACGCTGG GAAATCTCCT GCTCGAAATA GGTCAGCCAG CATTACCAAC 
CTCTCACTGG ATCGATCTGG TTCTCCCCTG GTACCTTCAT ATGAGACATC TGTCAGTCCC 
CAGGCCAACC GAACATACGT GAGGACAGAG ACCACAGAGG ATGAACGCAA AATTCTTCTG 
GACAGCGTCC AGTTAAAAGA CCTGTGGAAG AAAATCTGCC ATCACAGTAG TGGAATGGAG 
TTTCAGGATC ACCGCTACTG GCTGAGGACA CATCCCAACT GCATCGTAGG GAAGGAGTTA 
GTCAACTGGC TAATCCGAAA TGGGCACATT GCCACAAGGG CACAAGCTGT AGCAATTGGA 
CAAGCGATGG TGGACGGACG GTGGCTGGAC TGCGTGAGCC ACCATGACCA GCTCTTCAGG 
GATGAGTATG CGCTCTACAG ACCCCTGCAG AGTACAGACT TTTCTGAGAC CCCTTCTCCG 
GACAGTGACT CCGTGAACTC TGTGGAAGGA CACTCGGAGC CATCATGGTT TAAAGACATA 
AAGTTTGATG ACAGCGACAC AGAGCAGATT GCTGAAGAAG GTGACGATAA TTTGACTAAC 
TCTGCCAGTC CTAGCAAGCG CACGTCAGTC AGCAGTTTCC AGTCCACAGT GGACAGTGAC 
TCAGCCGCCT CCATCAGCCT GAACGTGGAG TTGGACAACG TGAACTTCCA CATCAAGAAG 
CCCTCCAAGT ACCCACACGT GCCCCCTCAC CCTGCTGACC AAAAAGGTAG GAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014588108.1
CDS	276..1952
Translation	MATDDKTSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPAFKSAYSSFVNLFRFNKDRAEGEQQSLTGGWPSPQLPSRTQSVRSPIPYKKQLTEELQRRPSAVLAENSLQHPQENTDTRRKTEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCRECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPLVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAVAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTDFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKGRR

Target ORF information:

RefSeq Version	XM_014732622.1
Organism	Equus caballus(horse)
Definition	Equus caballus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014732622.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGCAGA GAACAGTTTG CAGCACCCCC AGGAGAACAC AGACACAAGA 
AGGAAAACAG AACCTACCTT TGGCGGTCAT GACCCTCGTA CAGCTGTTCA GCTTCGAAGC 
CTCAGCACAG TGTTAAAACG CCTCAAGGAA ATCATGGAGG GGAAAAGCCA GGACAGTGAC 
CTGAAGCAGT ACTGGATGCC GGACAGCCAG TGCAGAGAGT GCTACGACTG CAGCGAGAAG 
TTCACCACCT TCCGGCGCAG GCACCACTGC CGGCTCTGCG GGCAGATTTT CTGCAGCCGC 
TGCTGCAATC AAGAGATCCC TGGGAAGTTT ATGGGCTATA CAGGGGACCT CCGAGCTTGC 
ACATACTGTA GAAAAATAGC CTTAAGTTAT GCTCATTCCA CAGACAGTAA TTCCATTGGG 
GAAGACTTGA ATGCTCTTTC AGATTCTGCT TCCTCTGTGT CTGTACTTGA TCCGAGTGAA 
CCCCGAACAC CTGTTGGGAG TAGGAAAGCC AGCCGGAACA TCTTTTTAGA GGATGATTTT 
GCCTGGCAAA GTTTGATTCA TCCAGACTCC TCAAATACTG CTCTTTCAAC AAGACTTGTA 
TCTGTTCAAG AGGACGCTGG GAAATCTCCT GCTCGAAATA GGTCAGCCAG CATTACCAAC 
CTCTCACTGG ATCGATCTGG TTCTCCCCTG GTACCTTCAT ATGAGACATC TGTCAGTCCC 
CAGGCCAACC GAACATACGT GAGGACAGAG ACCACAGAGG ATGAACGCAA AATTCTTCTG 
GACAGCGTCC AGTTAAAAGA CCTGTGGAAG AAAATCTGCC ATCACAGTAG TGGAATGGAG 
TTTCAGGATC ACCGCTACTG GCTGAGGACA CATCCCAACT GCATCGTAGG GAAGGAGTTA 
GTCAACTGGC TAATCCGAAA TGGGCACATT GCCACAAGGG CACAAGCTGT AGCAATTGGA 
CAAGCGATGG TGGACGGACG GTGGCTGGAC TGCGTGAGCC ACCATGACCA GCTCTTCAGG 
GATGAGTATG CGCTCTACAG ACCCCTGCAG AGTACAGACT TTTCTGAGAC CCCTTCTCCG 
GACAGTGACT CCGTGAACTC TGTGGAAGGA CACTCGGAGC CATCATGGTT TAAAGACATA 
AAGTTTGATG ACAGCGACAC AGAGCAGATT GCTGAAGAAG GTGACGATAA TTTGACTAAC 
TCTGCCAGTC CTAGCAAGCG CACGTCAGTC AGCAGTTTCC AGTCCACAGT GGACAGTGAC 
TCAGCCGCCT CCATCAGCCT GAACGTGGAG TTGGACAACG TGAACTTCCA CATCAAGAAG 
CCCTCCAAGT ACCCACACGT GCCCCCTCAC CCTGCTGACC AAAAAGGTAG GAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_023622531.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023478299.1 Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OHb36394
Clone ID Related Accession (Same CDS sequence)	XM_014732622.1 , XM_023622531.1
Accession Version	XM_023622531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGCAGA GAACAGTTTG CAGCACCCCC AGGAGAACAC AGACACAAGA 
AGGAAAACAG AACCTACCTT TGGCGGTCAT GACCCTCGTA CAGCTGTTCA GCTTCGAAGC 
CTCAGCACAG TGTTAAAACG CCTCAAGGAA ATCATGGAGG GGAAAAGCCA GGACAGTGAC 
CTGAAGCAGT ACTGGATGCC GGACAGCCAG TGCAGAGAGT GCTACGACTG CAGCGAGAAG 
TTCACCACCT TCCGGCGCAG GCACCACTGC CGGCTCTGCG GGCAGATTTT CTGCAGCCGC 
TGCTGCAATC AAGAGATCCC TGGGAAGTTT ATGGGCTATA CAGGGGACCT CCGAGCTTGC 
ACATACTGTA GAAAAATAGC CTTAAGTTAT GCTCATTCCA CAGACAGTAA TTCCATTGGG 
GAAGACTTGA ATGCTCTTTC AGATTCTGCT TCCTCTGTGT CTGTACTTGA TCCGAGTGAA 
CCCCGAACAC CTGTTGGGAG TAGGAAAGCC AGCCGGAACA TCTTTTTAGA GGATGATTTT 
GCCTGGCAAA GTTTGATTCA TCCAGACTCC TCAAATACTG CTCTTTCAAC AAGACTTGTA 
TCTGTTCAAG AGGACGCTGG GAAATCTCCT GCTCGAAATA GGTCAGCCAG CATTACCAAC 
CTCTCACTGG ATCGATCTGG TTCTCCCCTG GTACCTTCAT ATGAGACATC TGTCAGTCCC 
CAGGCCAACC GAACATACGT GAGGACAGAG ACCACAGAGG ATGAACGCAA AATTCTTCTG 
GACAGCGTCC AGTTAAAAGA CCTGTGGAAG AAAATCTGCC ATCACAGTAG TGGAATGGAG 
TTTCAGGATC ACCGCTACTG GCTGAGGACA CATCCCAACT GCATCGTAGG GAAGGAGTTA 
GTCAACTGGC TAATCCGAAA TGGGCACATT GCCACAAGGG CACAAGCTGT AGCAATTGGA 
CAAGCGATGG TGGACGGACG GTGGCTGGAC TGCGTGAGCC ACCATGACCA GCTCTTCAGG 
GATGAGTATG CGCTCTACAG ACCCCTGCAG AGTACAGACT TTTCTGAGAC CCCTTCTCCG 
GACAGTGACT CCGTGAACTC TGTGGAAGGA CACTCGGAGC CATCATGGTT TAAAGACATA 
AAGTTTGATG ACAGCGACAC AGAGCAGATT GCTGAAGAAG GTGACGATAA TTTGACTAAC 
TCTGCCAGTC CTAGCAAGCG CACGTCAGTC AGCAGTTTCC AGTCCACAGT GGACAGTGAC 
TCAGCCGCCT CCATCAGCCT GAACGTGGAG TTGGACAACG TGAACTTCCA CATCAAGAAG 
CCCTCCAAGT ACCCACACGT GCCCCCTCAC CCTGCTGACC AAAAAGGTAG GAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023478299.1
CDS	114..1790
Translation	MATDDKTSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPAFKSAYSSFVNLFRFNKDRAEGEQQSLTGGWPSPQLPSRTQSVRSPIPYKKQLTEELQRRPSAVLAENSLQHPQENTDTRRKTEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCRECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPLVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAVAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTDFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKGRR

Target ORF information:

RefSeq Version	XM_023622531.1
Organism	Equus caballus(horse)
Definition	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023622531.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGCAGA GAACAGTTTG CAGCACCCCC AGGAGAACAC AGACACAAGA 
AGGAAAACAG AACCTACCTT TGGCGGTCAT GACCCTCGTA CAGCTGTTCA GCTTCGAAGC 
CTCAGCACAG TGTTAAAACG CCTCAAGGAA ATCATGGAGG GGAAAAGCCA GGACAGTGAC 
CTGAAGCAGT ACTGGATGCC GGACAGCCAG TGCAGAGAGT GCTACGACTG CAGCGAGAAG 
TTCACCACCT TCCGGCGCAG GCACCACTGC CGGCTCTGCG GGCAGATTTT CTGCAGCCGC 
TGCTGCAATC AAGAGATCCC TGGGAAGTTT ATGGGCTATA CAGGGGACCT CCGAGCTTGC 
ACATACTGTA GAAAAATAGC CTTAAGTTAT GCTCATTCCA CAGACAGTAA TTCCATTGGG 
GAAGACTTGA ATGCTCTTTC AGATTCTGCT TCCTCTGTGT CTGTACTTGA TCCGAGTGAA 
CCCCGAACAC CTGTTGGGAG TAGGAAAGCC AGCCGGAACA TCTTTTTAGA GGATGATTTT 
GCCTGGCAAA GTTTGATTCA TCCAGACTCC TCAAATACTG CTCTTTCAAC AAGACTTGTA 
TCTGTTCAAG AGGACGCTGG GAAATCTCCT GCTCGAAATA GGTCAGCCAG CATTACCAAC 
CTCTCACTGG ATCGATCTGG TTCTCCCCTG GTACCTTCAT ATGAGACATC TGTCAGTCCC 
CAGGCCAACC GAACATACGT GAGGACAGAG ACCACAGAGG ATGAACGCAA AATTCTTCTG 
GACAGCGTCC AGTTAAAAGA CCTGTGGAAG AAAATCTGCC ATCACAGTAG TGGAATGGAG 
TTTCAGGATC ACCGCTACTG GCTGAGGACA CATCCCAACT GCATCGTAGG GAAGGAGTTA 
GTCAACTGGC TAATCCGAAA TGGGCACATT GCCACAAGGG CACAAGCTGT AGCAATTGGA 
CAAGCGATGG TGGACGGACG GTGGCTGGAC TGCGTGAGCC ACCATGACCA GCTCTTCAGG 
GATGAGTATG CGCTCTACAG ACCCCTGCAG AGTACAGACT TTTCTGAGAC CCCTTCTCCG 
GACAGTGACT CCGTGAACTC TGTGGAAGGA CACTCGGAGC CATCATGGTT TAAAGACATA 
AAGTTTGATG ACAGCGACAC AGAGCAGATT GCTGAAGAAG GTGACGATAA TTTGACTAAC 
TCTGCCAGTC CTAGCAAGCG CACGTCAGTC AGCAGTTTCC AGTCCACAGT GGACAGTGAC 
TCAGCCGCCT CCATCAGCCT GAACGTGGAG TTGGACAACG TGAACTTCCA CATCAAGAAG 
CCCTCCAAGT ACCCACACGT GCCCCCTCAC CCTGCTGACC AAAAAGGTAG GAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_023622532.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023478300.1 Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OHb52480
Clone ID Related Accession (Same CDS sequence)	XM_023622532.1
Accession Version	XM_023622532.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGAGAA CAGTTTGCAG CACCCCCAGG AGAACACAGA CACAAGAAGG 
AAAACAGAAC CTACCTTTGG CGGTCATGAC CCTCGTACAG CTGTTCAGCT TCGAAGCCTC 
AGCACAGTGT TAAAACGCCT CAAGGAAATC ATGGAGGGGA AAAGCCAGGA CAGTGACCTG 
AAGCAGTACT GGATGCCGGA CAGCCAGTGC AGAGAGTGCT ACGACTGCAG CGAGAAGTTC 
ACCACCTTCC GGCGCAGGCA CCACTGCCGG CTCTGCGGGC AGATTTTCTG CAGCCGCTGC 
TGCAATCAAG AGATCCCTGG GAAGTTTATG GGCTATACAG GGGACCTCCG AGCTTGCACA 
TACTGTAGAA AAATAGCCTT AAGTTATGCT CATTCCACAG ACAGTAATTC CATTGGGGAA 
GACTTGAATG CTCTTTCAGA TTCTGCTTCC TCTGTGTCTG TACTTGATCC GAGTGAACCC 
CGAACACCTG TTGGGAGTAG GAAAGCCAGC CGGAACATCT TTTTAGAGGA TGATTTTGCC 
TGGCAAAGTT TGATTCATCC AGACTCCTCA AATACTGCTC TTTCAACAAG ACTTGTATCT 
GTTCAAGAGG ACGCTGGGAA ATCTCCTGCT CGAAATAGGT CAGCCAGCAT TACCAACCTC 
TCACTGGATC GATCTGGTTC TCCCCTGGTA CCTTCATATG AGACATCTGT CAGTCCCCAG 
GCCAACCGAA CATACGTGAG GACAGAGACC ACAGAGGATG AACGCAAAAT TCTTCTGGAC 
AGCGTCCAGT TAAAAGACCT GTGGAAGAAA ATCTGCCATC ACAGTAGTGG AATGGAGTTT 
CAGGATCACC GCTACTGGCT GAGGACACAT CCCAACTGCA TCGTAGGGAA GGAGTTAGTC 
AACTGGCTAA TCCGAAATGG GCACATTGCC ACAAGGGCAC AAGCTGTAGC AATTGGACAA 
GCGATGGTGG ACGGACGGTG GCTGGACTGC GTGAGCCACC ATGACCAGCT CTTCAGGGAT 
GAGTATGCGC TCTACAGACC CCTGCAGAGT ACAGACTTTT CTGAGACCCC TTCTCCGGAC 
AGTGACTCCG TGAACTCTGT GGAAGGACAC TCGGAGCCAT CATGGTTTAA AGACATAAAG 
TTTGATGACA GCGACACAGA GCAGATTGCT GAAGAAGGTG ACGATAATTT GACTAACTCT 
GCCAGTCCTA GCAAGCGCAC GTCAGTCAGC AGTTTCCAGT CCACAGTGGA CAGTGACTCA 
GCCGCCTCCA TCAGCCTGAA CGTGGAGTTG GACAACGTGA ACTTCCACAT CAAGAAGCCC 
TCCAAGTACC CACACGTGCC CCCTCACCCT GCTGACCAAA AAGGTAGGAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023478300.1
CDS	114..1787
Translation	MATDDKTSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPAFKSAYSSFVNLFRFNKDRAEGEQQSLTGGWPSPQLPSRTQSVRSPIPYKKQLTEELQRRPSAVLENSLQHPQENTDTRRKTEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCRECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPLVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAVAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTDFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKGRR

Target ORF information:

RefSeq Version	XM_023622532.1
Organism	Equus caballus(horse)
Definition	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023622532.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGAGAA CAGTTTGCAG CACCCCCAGG AGAACACAGA CACAAGAAGG 
AAAACAGAAC CTACCTTTGG CGGTCATGAC CCTCGTACAG CTGTTCAGCT TCGAAGCCTC 
AGCACAGTGT TAAAACGCCT CAAGGAAATC ATGGAGGGGA AAAGCCAGGA CAGTGACCTG 
AAGCAGTACT GGATGCCGGA CAGCCAGTGC AGAGAGTGCT ACGACTGCAG CGAGAAGTTC 
ACCACCTTCC GGCGCAGGCA CCACTGCCGG CTCTGCGGGC AGATTTTCTG CAGCCGCTGC 
TGCAATCAAG AGATCCCTGG GAAGTTTATG GGCTATACAG GGGACCTCCG AGCTTGCACA 
TACTGTAGAA AAATAGCCTT AAGTTATGCT CATTCCACAG ACAGTAATTC CATTGGGGAA 
GACTTGAATG CTCTTTCAGA TTCTGCTTCC TCTGTGTCTG TACTTGATCC GAGTGAACCC 
CGAACACCTG TTGGGAGTAG GAAAGCCAGC CGGAACATCT TTTTAGAGGA TGATTTTGCC 
TGGCAAAGTT TGATTCATCC AGACTCCTCA AATACTGCTC TTTCAACAAG ACTTGTATCT 
GTTCAAGAGG ACGCTGGGAA ATCTCCTGCT CGAAATAGGT CAGCCAGCAT TACCAACCTC 
TCACTGGATC GATCTGGTTC TCCCCTGGTA CCTTCATATG AGACATCTGT CAGTCCCCAG 
GCCAACCGAA CATACGTGAG GACAGAGACC ACAGAGGATG AACGCAAAAT TCTTCTGGAC 
AGCGTCCAGT TAAAAGACCT GTGGAAGAAA ATCTGCCATC ACAGTAGTGG AATGGAGTTT 
CAGGATCACC GCTACTGGCT GAGGACACAT CCCAACTGCA TCGTAGGGAA GGAGTTAGTC 
AACTGGCTAA TCCGAAATGG GCACATTGCC ACAAGGGCAC AAGCTGTAGC AATTGGACAA 
GCGATGGTGG ACGGACGGTG GCTGGACTGC GTGAGCCACC ATGACCAGCT CTTCAGGGAT 
GAGTATGCGC TCTACAGACC CCTGCAGAGT ACAGACTTTT CTGAGACCCC TTCTCCGGAC 
AGTGACTCCG TGAACTCTGT GGAAGGACAC TCGGAGCCAT CATGGTTTAA AGACATAAAG 
TTTGATGACA GCGACACAGA GCAGATTGCT GAAGAAGGTG ACGATAATTT GACTAACTCT 
GCCAGTCCTA GCAAGCGCAC GTCAGTCAGC AGTTTCCAGT CCACAGTGGA CAGTGACTCA 
GCCGCCTCCA TCAGCCTGAA CGTGGAGTTG GACAACGTGA ACTTCCACAT CAAGAAGCCC 
TCCAAGTACC CACACGTGCC CCCTCACCCT GCTGACCAAA AAGGTAGGAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_023622530.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023478298.1 Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X14, mRNA.

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CloneID	OHb52479
Clone ID Related Accession (Same CDS sequence)	XM_023622530.1
Accession Version	XM_023622530.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AAAGCCAGGA CAGTGACCTG AAGCAGTACT GGATGCCGGA CAGCCAGTGC 
AGAGAGTGCT ACGACTGCAG CGAGAAGTTC ACCACCTTCC GGCGCAGGCA CCACTGCCGG 
CTCTGCGGGC AGATTTTCTG CAGCCGCTGC TGCAATCAAG AGATCCCTGG GAAGTTTATG 
GGCTATACAG GGGACCTCCG AGCTTGCACA TACTGTAGAA AAATAGCCTT AAGTTATGCT 
CATTCCACAG ACAGTAATTC CATTGGGGAA GACTTGAATG CTCTTTCAGA TTCTGCTTCC 
TCTGTGTCTG TACTTGATCC GAGTGAACCC CGAACACCTG TTGGGAGTAG GAAAGCCAGC 
CGGAACATCT TTTTAGAGGA TGATTTTGCC TGGCAAAGTT TGATTCATCC AGACTCCTCA 
AATACTGCTC TTTCAACAAG ACTTGTATCT GTTCAAGAGG ACGCTGGGAA ATCTCCTGCT 
CGAAATAGAA TGGACTTGGC AGTGCCTGCT CCATTGGATG CTGTGTGGAT TAAATCATAC 
ATACATGGCC CTACTGGGTC AGCCAGCATT ACCAACCTCT CACTGGATCG ATCTGGTTCT 
CCCCTGGTAC CTTCATATGA GACATCTGTC AGTCCCCAGG CCAACCGAAC ATACGTGAGG 
ACAGAGACCA CAGAGGATGA ACGCAAAATT CTTCTGGACA GCGTCCAGTT AAAAGACCTG 
TGGAAGAAAA TCTGCCATCA CAGTAGTGGA ATGGAGTTTC AGGATCACCG CTACTGGCTG 
AGGACACATC CCAACTGCAT CGTAGGGAAG GAGTTAGTCA ACTGGCTAAT CCGAAATGGG 
CACATTGCCA CAAGGGCACA AGCTGTAGCA ATTGGACAAG CGATGGTGGA CGGACGGTGG 
CTGGACTGCG TGAGCCACCA TGACCAGCTC TTCAGGGATG AGTATGCGCT CTACAGACCC 
CTGCAGAGTA CAGACTTTTC TGAGACCCCT TCTCCGGACA GTGACTCCGT GAACTCTGTG 
GAAGGACACT CGGAGCCATC ATGGTTTAAA GACATAAAGT TTGATGACAG CGACACAGAG 
CAGATTGCTG AAGAAGGTGA CGATAATTTG ACTAACTCTG CCAGTCCTAG CAAGCGCACG 
TCAGTCAGCA GTTTCCAGTC CACAGTGGAC AGTGACTCAG CCGCCTCCAT CAGCCTGAAC 
GTGGAGTTGG ACAACGTGAA CTTCCACATC AAGAAGCCCT CCAAGTACCC ACACGTGCCC 
CCTCACCCTG CTGACCAAAA AGAGTATTTG ATTTCTGACA ACGGAGGACA ACAACTCTCA 
ATAAGTGACG CCTTCATCAA AGAATCCTTA TTTAATCGTC GAGTTGAGGA GAAATCCAAA 
GAACTGCCCT TTACGCCTTT GGGCTGGCAT CATAACAATC TGGAGCTGCT GCGGGAGGAG 
AATGGAGAGA AGCAGGCCAT GGAGAGGTTG CTTTCAGCTA ATCATAACCA CATGATGGCA 
CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA TTGTCACCAT CTTGGAGGGA CATCATTGTA 
TCCCTGGTCT GCCAGGTTGT TCAGACAGTC CGACCTGACG TCAAGAACCG GGATGACGAC 
ATGGATATCC GTCAGTTTGT CCACGTCAAA AAAATCCCAG GTGGAAAGAA GTTTGATTCT 
ATGGTTGTCA ATGGGTTCGT TTGTACCAAG AATATTGCAC ATAAAAAGAT GAATTCTTGT 
ATTAAAAACC CCAAAATTCT TCTGTTGAAG TGTTCCATTG AGTATCTCTA CAGAGAAGAA 
ACTAAATTTA CTTGCATTGA CCCTATCGTG CTTCAGGAAA GGGAATTCTT GAAGAATTAT 
GTTCAGCGAA TAGTGGATGT TCGACCCACA TTGGTTCTAG TTGAGAAAAC GGTGTCTCGG 
ATTGCCCAGG ACCTGTTGCT GGAACACGGC ATCACTTTGG TCATTAATGT CAAATCACAA 
GTTTTAGAAC GAATCAGTCG GATGACCCAA GGTGATTTAG TGATGTCGAT GGACCAGTTG 
CTCACAAAGC CCCACCTGGG CACCTGTCAC AAATTTTACA TGCAGATGTT TCAGCTACCT 
GGTGAACAAA CCAAAACTCT GATGTTTTTT GAAGGCTGTC CACAGCACCT GGGCTGTACA 
ATCAAGCTTA GAGGAGGCTC CGATTATGAG CTGGCTCGAG TTAAGGAGAT CCTCATATTT 
ATGATCTGTG TAGCATATCA TTCTCAACTA GAAATCTCCT TCCTCATGGA TGAATTTGCC 
ATGCCTCCTA CATTAACGCA AAACCCTTCC TTCCACTCTC TGATTGAGGA ACAGGAGGAT 
GACGAGGCTG CTCAAGAACA GCTCAGCGGA AGTCCCCTCC CCCGGGAACC TGACTTCCCT 
CTGGAGTGTC TGGCCCCTGA TGAGAGCAGT TCACTGGAAT CAAGGACTGT GTTTGGGAAG 
AGTGACCAGG AACTTAAAGG TGCTCCAAAG GATGCTGTCT CGTTGAAGCA GCAGGAGTTC 
GCCACAGCAG CCTGCCCGGC CGGCGTCCCC TATGCTCTCT TTGCATTGGT CCCAGAATCC 
TTGTTGCCAC TGCATCTGGG TGACCAACAG GATGCCAGGG GCAGCGAGCA GCCAGAGACT 
GTGCAGCAAA CGGGTGAGCT ACAGGATCCC AAAAGCCAGA TGAGAGCCTT TAGAGACCCT 
CTGCAGGATG ACACTGGATT GTATGTCACT GAGGAAGTCA CCTCTTCTGA GGATAAGCGG 
AAGACTTACT CTTTGGCCTT TAAACAGGAA TTAAAAGACG TAATTCTCTG TATCTCCCCA 
GTGATCACAT TCCGAGAGCC TTTCCTTTTA ACCGAGAAGG GGATGCGATG CTCTCCCCGA 
GACTATTTCG CAGAGCAGCT TTACTGGTCT CCTCTCCTCA ATAAAGACTT CAAAGAAATG 
GAGAGCAGGC GGAAGAAGCA GCTGCTCAGG GATCTCTCCA GCCTCCAGGG CCTGAACGGA 
AGTGTGCAGG CCAAGTCTAT CCAAGTGCTG CCCTCACACG AGCTGGTGAG CGCTAGGATT 
GCTGAGCATC TGGGTGACAG CCAGGGCCTG GGCCGGATGC TGGCTGACTA CCGGGCCAGA 
GGAGGGAGAA TTCAGCAGAA TACTTCAGAC CCTTTTGCTC ATTCAAAGGA TGCATCAGGT 
CCTTCAAGTG GCAAGTCAGG AAGCAGAACT GAGGGCGATG AAGAGAAAGG GCTGATTCCG 
AGTGACGCGG TATGGTCAAC AAAGGTGGAC TGTCTGAACC CTGCCAACCA CCAGAGGCTG 
TGCGTGCTCT TCAGCAGCTC TTCTGCCCAG TCCAGCAACG CGCCCAGCGC CTGCGTCAGT 
CCTTGGATTG TAACAATGGA ATTTTATGGA AAGAATGATC TTACACTGGG AATATTTTTA 
GAGAGATACT GTTTCAGGCC TTCTTACCAG TGTCCTAGCA TGTTCTGTGA TACCCCCATG 
GTACATCACA TTCGGCGCTT TGTCCATGGC CAAGGCTGTG TGCAGATAAT CCTGAAGGAG 
CTGGATTCTC CAGTACCTGG ATATCAGCAT ACAATTCTCA CCTATTCCTG GTGCAGAATC 
TGCAAACAGG TAACACCAGT TGTTGCTCTT TCCAATGAGT CCTGGTCCAT GTCATTTGCC 
AAATACCTTG AACTTCGGTT TTATGGGCAC CAGTACACCC GTAGAGCCAA TGCTGAGCCG 
TGTGGCCACT CCATCCACCA TGATTATCAC CAGTACTTCT CCTATAACCA GATGGTGGCA 
TCTTTTAGTT ATTCTCCCAT TCGGCTTCTT GAAGTATGTG TTCCACTCCC CAAAATATTC 
ATTAAACGTC AGGCCCCATT AAAAGTGTCC CTTCTTCAAG ATCTGAAGGA CTTCTTTCAA 
AAAGTTTCAC AAGTATATCT TGCTGTTGAT GAAAGACTTG CATCTTTGAA AACTGATACA 
TTTAGCAAAA CAAGAGAGGA AAAAATGGAA GATATCTTTG CACAAAAAGA GATGGAGGAA 
GGCGAGTTCA AGAACTGGAT TGAGAAAATG CAAGCGAGGC TCCTGTCTTC CTCGGTAGAC 
ACCCCTCAGC AGCTGCAGTC GGTCCTGGAG TCGCTCATCG CCAAGAAGCA GAGCCTCTGT 
GAAGTGCTGC AGGCCTGGAA TAACAGGTTG CAGGACCTTT TCCAGCAGGA AAAGGGTAGA 
AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA AGACTGAGAC AAGGGGAAGA AAGCAAGATA 
AGTGCAATGG ATGCATCTCC ACGGAATATT TCTCCAGGAC TTCAAAATGG AGAAAAAGAG 
GACCGCTTCT TGACAACCCT CTCTAGTCAG GGCCCCACCT CTGCTGCTCA TCTCCAGCTG 
CCCAGTCCCC CTGAAGTCCT GCCTGAGCAG GCAGGGGGGG CGACCCCCGA GCCGGATGCA 
GCCAGCAGTT CTGAAGATGT GTTTGATGGA CATTCTCTGG GATCCACAGA CAGCCAGGTG 
AAGGAAAAAT CAACCATGAA GGCCATCTTT GCAAATTTGC TTCCAGGAAA TAGCTATAAC 
CCTATTCCGT TTCCTTTTGA TCCAGATAAA CACTACTTAA TGTACGAACA CGAACGGGTG 
CCCATTGCCG TCTGTGAGAG GGAGCCCAGC TCCATCATTG CTTTTGCTCT CAGTTGTAAA 
GAATACCGAA ATGCCTTAGA GGAATTGTCC AAGGCAACTC TGCGGAACAG TGCCGAAGAA 
GGGCTCCCAG CCAACAGTGC TTCAGACAGC AGACCAAAGG GCGGCAGCCC TGGGAGGCCG 
CCTGAGCTCG GTGGAGGCTG CCCCAACCGG GCGGCCGAGG CAGAGCCGCA GCCAGCCAAA 
AAGGCTTCTG GGATGTTGTC CTTCTTCCGA GGGACAGCAG GGAAAAGCCC TGATCTCTCT 
TCCCAGAAGA GAGAGACCTT ACGTGGAGCA GATAGTGCTT ACTACCAGGT CGGGCAGCCC 
GGCAAGGAGG GGACGGAGAG TCAAGGCACT GAGCCTCCAG ATGAGATAGA TGGAGGAGAC 
GCACAAAAGA AACAGCTCAC AAATCCTCAT GTGGAACTTC AATTTTCTGA CGCTAACGCC 
AAGTTTTACT GCCGGCTCTA CTATGCGGGA GAGTTTCACA AGATGCGCGA GGTGATCCTG 
GGCAGCAGCG AGGAGGACTT CATCCGCTCC CTTTCCCACT CGGCACCCTG GCAGGCACGC 
GGGGGCAAGT CCGGAGCGGC CTTCTACGCC ACGGAAGATG ATAGATTCAT TTTGAAGCAG 
ATGCCTCGTC TGGAAGTCCA GTCTTTCCTC GACTTTGCAC CACACTACTT CAATTATATC 
ACAAACGCTG TGCAACAAAA GAGGCCTACT GCACTGGCCA AAATTCTTGG AGTTTACAGA 
ATTGGTTATA AGAATTCTCA GAACAACACT GAGAAGAAGT TAGATCTCCT TGTCATGGAA 
AATCTTTTCT ATGGGAGAAA GATGGCACAG GTTTTTGACT TGAAGGGTTC ACTTAGGAAC 
AGAAATGTAA AAACTGACAC CGGGAAAGAG AGCTGTGACG TGGTCCTGCT GGACGAGAAT 
CTGCTGAAGA TGGTCCGGGA CAGCCCTCTG TACATCCGCT CGCACTCCAA GGCGGTGCTG 
CGGGCCTCCA TCCGCAGCGA TGCCCACTTC CTGTCCAGCC ACCTCATCAT CGACTATTCT 
TTGCTGGTGG GGCGAGATGA TACCAGCAAT GAGCTTGTCG TTGGGATTAT AGATTATATT 
CGAACGTTTA CATGGGACAA AAAGCTTGAG ATGGTTGTGA AATCAACAGG AATTTTAGGA 
GGACAAGGTA AAATGCCAAC TGTGGTCTCT CCAGAGTTGT ACAGGACCCG ATTTTGTGAA 
GCGATGGACA AGTATTTCCT CATGGTACCA GACCACTGGA CAGGCTTGGG TCTCAACTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023478298.1
CDS	411..6353
Translation	MEGKSQDSDLKQYWMPDSQCRECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRMDLAVPAPLDAVWIKSYIHGPTGSASITNLSLDRSGSPLVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAVAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTDFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSPSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHVKKIPGGKKFDSMVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDLLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPGEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEEQEDDEAAQEQLSGSPLPREPDFPLECLAPDESSSLESRTVFGKSDQELKGAPKDAVSLKQQEFATAACPAGVPYALFALVPESLLPLHLGDQQDARGSEQPETVQQTGELQDPKSQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSPRDYFAEQLYWSPLLNKDFKEMESRRKKQLLRDLSSLQGLNGSVQAKSIQVLPSHELVSARIAEHLGDSQGLGRMLADYRARGGRIQQNTSDPFAHSKDASGPSSGKSGSRTEGDEEKGLIPSDAVWSTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLLSSSVDTPQQLQSVLESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQGPTSAAHLQLPSPPEVLPEQAGGATPEPDAASSSEDVFDGHSLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSASDSRPKGGSPGRPPELGGGCPNRAAEAEPQPAKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQPGKEGTESQGTEPPDEIDGGDAQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSAPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDSPLYIRSHSKAVLRASIRSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_023622530.1
Organism	Equus caballus(horse)
Definition	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023622530.1

ORF Insert Sequence:

ATGGAGGGGA AAAGCCAGGA CAGTGACCTG AAGCAGTACT GGATGCCGGA CAGCCAGTGC 
AGAGAGTGCT ACGACTGCAG CGAGAAGTTC ACCACCTTCC GGCGCAGGCA CCACTGCCGG 
CTCTGCGGGC AGATTTTCTG CAGCCGCTGC TGCAATCAAG AGATCCCTGG GAAGTTTATG 
GGCTATACAG GGGACCTCCG AGCTTGCACA TACTGTAGAA AAATAGCCTT AAGTTATGCT 
CATTCCACAG ACAGTAATTC CATTGGGGAA GACTTGAATG CTCTTTCAGA TTCTGCTTCC 
TCTGTGTCTG TACTTGATCC GAGTGAACCC CGAACACCTG TTGGGAGTAG GAAAGCCAGC 
CGGAACATCT TTTTAGAGGA TGATTTTGCC TGGCAAAGTT TGATTCATCC AGACTCCTCA 
AATACTGCTC TTTCAACAAG ACTTGTATCT GTTCAAGAGG ACGCTGGGAA ATCTCCTGCT 
CGAAATAGAA TGGACTTGGC AGTGCCTGCT CCATTGGATG CTGTGTGGAT TAAATCATAC 
ATACATGGCC CTACTGGGTC AGCCAGCATT ACCAACCTCT CACTGGATCG ATCTGGTTCT 
CCCCTGGTAC CTTCATATGA GACATCTGTC AGTCCCCAGG CCAACCGAAC ATACGTGAGG 
ACAGAGACCA CAGAGGATGA ACGCAAAATT CTTCTGGACA GCGTCCAGTT AAAAGACCTG 
TGGAAGAAAA TCTGCCATCA CAGTAGTGGA ATGGAGTTTC AGGATCACCG CTACTGGCTG 
AGGACACATC CCAACTGCAT CGTAGGGAAG GAGTTAGTCA ACTGGCTAAT CCGAAATGGG 
CACATTGCCA CAAGGGCACA AGCTGTAGCA ATTGGACAAG CGATGGTGGA CGGACGGTGG 
CTGGACTGCG TGAGCCACCA TGACCAGCTC TTCAGGGATG AGTATGCGCT CTACAGACCC 
CTGCAGAGTA CAGACTTTTC TGAGACCCCT TCTCCGGACA GTGACTCCGT GAACTCTGTG 
GAAGGACACT CGGAGCCATC ATGGTTTAAA GACATAAAGT TTGATGACAG CGACACAGAG 
CAGATTGCTG AAGAAGGTGA CGATAATTTG ACTAACTCTG CCAGTCCTAG CAAGCGCACG 
TCAGTCAGCA GTTTCCAGTC CACAGTGGAC AGTGACTCAG CCGCCTCCAT CAGCCTGAAC 
GTGGAGTTGG ACAACGTGAA CTTCCACATC AAGAAGCCCT CCAAGTACCC ACACGTGCCC 
CCTCACCCTG CTGACCAAAA AGAGTATTTG ATTTCTGACA ACGGAGGACA ACAACTCTCA 
ATAAGTGACG CCTTCATCAA AGAATCCTTA TTTAATCGTC GAGTTGAGGA GAAATCCAAA 
GAACTGCCCT TTACGCCTTT GGGCTGGCAT CATAACAATC TGGAGCTGCT GCGGGAGGAG 
AATGGAGAGA AGCAGGCCAT GGAGAGGTTG CTTTCAGCTA ATCATAACCA CATGATGGCA 
CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA TTGTCACCAT CTTGGAGGGA CATCATTGTA 
TCCCTGGTCT GCCAGGTTGT TCAGACAGTC CGACCTGACG TCAAGAACCG GGATGACGAC 
ATGGATATCC GTCAGTTTGT CCACGTCAAA AAAATCCCAG GTGGAAAGAA GTTTGATTCT 
ATGGTTGTCA ATGGGTTCGT TTGTACCAAG AATATTGCAC ATAAAAAGAT GAATTCTTGT 
ATTAAAAACC CCAAAATTCT TCTGTTGAAG TGTTCCATTG AGTATCTCTA CAGAGAAGAA 
ACTAAATTTA CTTGCATTGA CCCTATCGTG CTTCAGGAAA GGGAATTCTT GAAGAATTAT 
GTTCAGCGAA TAGTGGATGT TCGACCCACA TTGGTTCTAG TTGAGAAAAC GGTGTCTCGG 
ATTGCCCAGG ACCTGTTGCT GGAACACGGC ATCACTTTGG TCATTAATGT CAAATCACAA 
GTTTTAGAAC GAATCAGTCG GATGACCCAA GGTGATTTAG TGATGTCGAT GGACCAGTTG 
CTCACAAAGC CCCACCTGGG CACCTGTCAC AAATTTTACA TGCAGATGTT TCAGCTACCT 
GGTGAACAAA CCAAAACTCT GATGTTTTTT GAAGGCTGTC CACAGCACCT GGGCTGTACA 
ATCAAGCTTA GAGGAGGCTC CGATTATGAG CTGGCTCGAG TTAAGGAGAT CCTCATATTT 
ATGATCTGTG TAGCATATCA TTCTCAACTA GAAATCTCCT TCCTCATGGA TGAATTTGCC 
ATGCCTCCTA CATTAACGCA AAACCCTTCC TTCCACTCTC TGATTGAGGA ACAGGAGGAT 
GACGAGGCTG CTCAAGAACA GCTCAGCGGA AGTCCCCTCC CCCGGGAACC TGACTTCCCT 
CTGGAGTGTC TGGCCCCTGA TGAGAGCAGT TCACTGGAAT CAAGGACTGT GTTTGGGAAG 
AGTGACCAGG AACTTAAAGG TGCTCCAAAG GATGCTGTCT CGTTGAAGCA GCAGGAGTTC 
GCCACAGCAG CCTGCCCGGC CGGCGTCCCC TATGCTCTCT TTGCATTGGT CCCAGAATCC 
TTGTTGCCAC TGCATCTGGG TGACCAACAG GATGCCAGGG GCAGCGAGCA GCCAGAGACT 
GTGCAGCAAA CGGGTGAGCT ACAGGATCCC AAAAGCCAGA TGAGAGCCTT TAGAGACCCT 
CTGCAGGATG ACACTGGATT GTATGTCACT GAGGAAGTCA CCTCTTCTGA GGATAAGCGG 
AAGACTTACT CTTTGGCCTT TAAACAGGAA TTAAAAGACG TAATTCTCTG TATCTCCCCA 
GTGATCACAT TCCGAGAGCC TTTCCTTTTA ACCGAGAAGG GGATGCGATG CTCTCCCCGA 
GACTATTTCG CAGAGCAGCT TTACTGGTCT CCTCTCCTCA ATAAAGACTT CAAAGAAATG 
GAGAGCAGGC GGAAGAAGCA GCTGCTCAGG GATCTCTCCA GCCTCCAGGG CCTGAACGGA 
AGTGTGCAGG CCAAGTCTAT CCAAGTGCTG CCCTCACACG AGCTGGTGAG CGCTAGGATT 
GCTGAGCATC TGGGTGACAG CCAGGGCCTG GGCCGGATGC TGGCTGACTA CCGGGCCAGA 
GGAGGGAGAA TTCAGCAGAA TACTTCAGAC CCTTTTGCTC ATTCAAAGGA TGCATCAGGT 
CCTTCAAGTG GCAAGTCAGG AAGCAGAACT GAGGGCGATG AAGAGAAAGG GCTGATTCCG 
AGTGACGCGG TATGGTCAAC AAAGGTGGAC TGTCTGAACC CTGCCAACCA CCAGAGGCTG 
TGCGTGCTCT TCAGCAGCTC TTCTGCCCAG TCCAGCAACG CGCCCAGCGC CTGCGTCAGT 
CCTTGGATTG TAACAATGGA ATTTTATGGA AAGAATGATC TTACACTGGG AATATTTTTA 
GAGAGATACT GTTTCAGGCC TTCTTACCAG TGTCCTAGCA TGTTCTGTGA TACCCCCATG 
GTACATCACA TTCGGCGCTT TGTCCATGGC CAAGGCTGTG TGCAGATAAT CCTGAAGGAG 
CTGGATTCTC CAGTACCTGG ATATCAGCAT ACAATTCTCA CCTATTCCTG GTGCAGAATC 
TGCAAACAGG TAACACCAGT TGTTGCTCTT TCCAATGAGT CCTGGTCCAT GTCATTTGCC 
AAATACCTTG AACTTCGGTT TTATGGGCAC CAGTACACCC GTAGAGCCAA TGCTGAGCCG 
TGTGGCCACT CCATCCACCA TGATTATCAC CAGTACTTCT CCTATAACCA GATGGTGGCA 
TCTTTTAGTT ATTCTCCCAT TCGGCTTCTT GAAGTATGTG TTCCACTCCC CAAAATATTC 
ATTAAACGTC AGGCCCCATT AAAAGTGTCC CTTCTTCAAG ATCTGAAGGA CTTCTTTCAA 
AAAGTTTCAC AAGTATATCT TGCTGTTGAT GAAAGACTTG CATCTTTGAA AACTGATACA 
TTTAGCAAAA CAAGAGAGGA AAAAATGGAA GATATCTTTG CACAAAAAGA GATGGAGGAA 
GGCGAGTTCA AGAACTGGAT TGAGAAAATG CAAGCGAGGC TCCTGTCTTC CTCGGTAGAC 
ACCCCTCAGC AGCTGCAGTC GGTCCTGGAG TCGCTCATCG CCAAGAAGCA GAGCCTCTGT 
GAAGTGCTGC AGGCCTGGAA TAACAGGTTG CAGGACCTTT TCCAGCAGGA AAAGGGTAGA 
AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA AGACTGAGAC AAGGGGAAGA AAGCAAGATA 
AGTGCAATGG ATGCATCTCC ACGGAATATT TCTCCAGGAC TTCAAAATGG AGAAAAAGAG 
GACCGCTTCT TGACAACCCT CTCTAGTCAG GGCCCCACCT CTGCTGCTCA TCTCCAGCTG 
CCCAGTCCCC CTGAAGTCCT GCCTGAGCAG GCAGGGGGGG CGACCCCCGA GCCGGATGCA 
GCCAGCAGTT CTGAAGATGT GTTTGATGGA CATTCTCTGG GATCCACAGA CAGCCAGGTG 
AAGGAAAAAT CAACCATGAA GGCCATCTTT GCAAATTTGC TTCCAGGAAA TAGCTATAAC 
CCTATTCCGT TTCCTTTTGA TCCAGATAAA CACTACTTAA TGTACGAACA CGAACGGGTG 
CCCATTGCCG TCTGTGAGAG GGAGCCCAGC TCCATCATTG CTTTTGCTCT CAGTTGTAAA 
GAATACCGAA ATGCCTTAGA GGAATTGTCC AAGGCAACTC TGCGGAACAG TGCCGAAGAA 
GGGCTCCCAG CCAACAGTGC TTCAGACAGC AGACCAAAGG GCGGCAGCCC TGGGAGGCCG 
CCTGAGCTCG GTGGAGGCTG CCCCAACCGG GCGGCCGAGG CAGAGCCGCA GCCAGCCAAA 
AAGGCTTCTG GGATGTTGTC CTTCTTCCGA GGGACAGCAG GGAAAAGCCC TGATCTCTCT 
TCCCAGAAGA GAGAGACCTT ACGTGGAGCA GATAGTGCTT ACTACCAGGT CGGGCAGCCC 
GGCAAGGAGG GGACGGAGAG TCAAGGCACT GAGCCTCCAG ATGAGATAGA TGGAGGAGAC 
GCACAAAAGA AACAGCTCAC AAATCCTCAT GTGGAACTTC AATTTTCTGA CGCTAACGCC 
AAGTTTTACT GCCGGCTCTA CTATGCGGGA GAGTTTCACA AGATGCGCGA GGTGATCCTG 
GGCAGCAGCG AGGAGGACTT CATCCGCTCC CTTTCCCACT CGGCACCCTG GCAGGCACGC 
GGGGGCAAGT CCGGAGCGGC CTTCTACGCC ACGGAAGATG ATAGATTCAT TTTGAAGCAG 
ATGCCTCGTC TGGAAGTCCA GTCTTTCCTC GACTTTGCAC CACACTACTT CAATTATATC 
ACAAACGCTG TGCAACAAAA GAGGCCTACT GCACTGGCCA AAATTCTTGG AGTTTACAGA 
ATTGGTTATA AGAATTCTCA GAACAACACT GAGAAGAAGT TAGATCTCCT TGTCATGGAA 
AATCTTTTCT ATGGGAGAAA GATGGCACAG GTTTTTGACT TGAAGGGTTC ACTTAGGAAC 
AGAAATGTAA AAACTGACAC CGGGAAAGAG AGCTGTGACG TGGTCCTGCT GGACGAGAAT 
CTGCTGAAGA TGGTCCGGGA CAGCCCTCTG TACATCCGCT CGCACTCCAA GGCGGTGCTG 
CGGGCCTCCA TCCGCAGCGA TGCCCACTTC CTGTCCAGCC ACCTCATCAT CGACTATTCT 
TTGCTGGTGG GGCGAGATGA TACCAGCAAT GAGCTTGTCG TTGGGATTAT AGATTATATT 
CGAACGTTTA CATGGGACAA AAAGCTTGAG ATGGTTGTGA AATCAACAGG AATTTTAGGA 
GGACAAGGTA AAATGCCAAC TGTGGTCTCT CCAGAGTTGT ACAGGACCCG ATTTTGTGAA 
GCGATGGACA AGTATTTCCT CATGGTACCA GACCACTGGA CAGGCTTGGG TCTCAACTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_023622519.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023478287.1 Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X3, mRNA.

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CloneID	OHb52469
Clone ID Related Accession (Same CDS sequence)	XM_023622519.1 , XM_023622517.1 , XM_023622518.1 , XM_023622520.1
Accession Version	XM_023622519.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGCAGA GAACAGTTTG CAGCACCCCC AGGAGAACAC AGACACAAGA 
AGGAAAACAG AACCTACCTT TGGCGGTCAT GACCCTCGTA CAGCTGTTCA GCTTCGAAGC 
CTCAGCACAG TGTTAAAACG CCTCAAGGAA ATCATGGAGG GGAAAAGCCA GGACAGTGAC 
CTGAAGCAGT ACTGGATGCC GGACAGCCAG TGCAGAGAGT GCTACGACTG CAGCGAGAAG 
TTCACCACCT TCCGGCGCAG GCACCACTGC CGGCTCTGCG GGCAGATTTT CTGCAGCCGC 
TGCTGCAATC AAGAGATCCC TGGGAAGTTT ATGGGCTATA CAGGGGACCT CCGAGCTTGC 
ACATACTGTA GAAAAATAGC CTTAAGTTAT GCTCATTCCA CAGACAGTAA TTCCATTGGG 
GAAGACTTGA ATGCTCTTTC AGATTCTGCT TCCTCTGTGT CTGTACTTGA TCCGAGTGAA 
CCCCGAACAC CTGTTGGGAG TAGGAAAGCC AGCCGGAACA TCTTTTTAGA GGATGATTTT 
GCCTGGCAAA GTTTGATTCA TCCAGACTCC TCAAATACTG CTCTTTCAAC AAGACTTGTA 
TCTGTTCAAG AGGACGCTGG GAAATCTCCT GCTCGAAATA GAATGGACTT GGCAGTGCCT 
GCTCCATTGG ATGCTGTGTG GATTAAATCA TACATACATG GCCCTACTGG GTCAGCCAGC 
ATTACCAACC TCTCACTGGA TCGATCTGGT TCTCCCCTGG TACCTTCATA TGAGACATCT 
GTCAGTCCCC AGGCCAACCG AACATACGTG AGGACAGAGA CCACAGAGGA TGAACGCAAA 
ATTCTTCTGG ACAGCGTCCA GTTAAAAGAC CTGTGGAAGA AAATCTGCCA TCACAGTAGT 
GGAATGGAGT TTCAGGATCA CCGCTACTGG CTGAGGACAC ATCCCAACTG CATCGTAGGG 
AAGGAGTTAG TCAACTGGCT AATCCGAAAT GGGCACATTG CCACAAGGGC ACAAGCTGTA 
GCAATTGGAC AAGCGATGGT GGACGGACGG TGGCTGGACT GCGTGAGCCA CCATGACCAG 
CTCTTCAGGG ATGAGTATGC GCTCTACAGA CCCCTGCAGA GTACAGACTT TTCTGAGACC 
CCTTCTCCGG ACAGTGACTC CGTGAACTCT GTGGAAGGAC ACTCGGAGCC ATCATGGTTT 
AAAGACATAA AGTTTGATGA CAGCGACACA GAGCAGATTG CTGAAGAAGG TGACGATAAT 
TTGACTAACT CTGCCAGTCC TAGCAAGCGC ACGTCAGTCA GCAGTTTCCA GTCCACAGTG 
GACAGTGACT CAGCCGCCTC CATCAGCCTG AACGTGGAGT TGGACAACGT GAACTTCCAC 
ATCAAGAAGC CCTCCAAGTA CCCACACGTG CCCCCTCACC CTGCTGACCA AAAAGAGTAT 
TTGATTTCTG ACAACGGAGG ACAACAACTC TCAATAAGTG ACGCCTTCAT CAAAGAATCC 
TTATTTAATC GTCGAGTTGA GGAGAAATCC AAAGAACTGC CCTTTACGCC TTTGGGCTGG 
CATCATAACA ATCTGGAGCT GCTGCGGGAG GAGAATGGAG AGAAGCAGGC CATGGAGAGG 
TTGCTTTCAG CTAATCATAA CCACATGATG GCACTGCTCC AACAGTTGCT CCATAGTGAG 
TCATTGTCAC CATCTTGGAG GGACATCATT GTATCCCTGG TCTGCCAGGT TGTTCAGACA 
GTCCGACCTG ACGTCAAGAA CCGGGATGAC GACATGGATA TCCGTCAGTT TGTCCACGTC 
AAAAAAATCC CAGGTGGAAA GAAGTTTGAT TCTATGGTTG TCAATGGGTT CGTTTGTACC 
AAGAATATTG CACATAAAAA GATGAATTCT TGTATTAAAA ACCCCAAAAT TCTTCTGTTG 
AAGTGTTCCA TTGAGTATCT CTACAGAGAA GAAACTAAAT TTACTTGCAT TGACCCTATC 
GTGCTTCAGG AAAGGGAATT CTTGAAGAAT TATGTTCAGC GAATAGTGGA TGTTCGACCC 
ACATTGGTTC TAGTTGAGAA AACGGTGTCT CGGATTGCCC AGGACCTGTT GCTGGAACAC 
GGCATCACTT TGGTCATTAA TGTCAAATCA CAAGTTTTAG AACGAATCAG TCGGATGACC 
CAAGGTGATT TAGTGATGTC GATGGACCAG TTGCTCACAA AGCCCCACCT GGGCACCTGT 
CACAAATTTT ACATGCAGAT GTTTCAGCTA CCTGGTGAAC AAACCAAAAC TCTGATGTTT 
TTTGAAGGCT GTCCACAGCA CCTGGGCTGT ACAATCAAGC TTAGAGGAGG CTCCGATTAT 
GAGCTGGCTC GAGTTAAGGA GATCCTCATA TTTATGATCT GTGTAGCATA TCATTCTCAA 
CTAGAAATCT CCTTCCTCAT GGATGAATTT GCCATGCCTC CTACATTAAC GCAAAACCCT 
TCCTTCCACT CTCTGATTGA GGAACAGGAG GATGACGAGG CTGCTCAAGA ACAGCTCAGC 
GGAAGTCCCC TCCCCCGGGA ACCTGACTTC CCTCTGGAGT GTCTGGCCCC TGATGAGAGC 
AGTTCACTGG AATCAAGGAC TGTGTTTGGG AAGAGTGACC AGGAACTTAA AGGTGCTCCA 
AAGGATGCTG TCTCGTTGAA GCAGCAGGAG TTCGCCACAG CAGCCTGCCC GGCCGGCGTC 
CCCTATGCTC TCTTTGCATT GGTCCCAGAA TCCTTGTTGC CACTGCATCT GGGTGACCAA 
CAGGATGCCA GGGGCAGCGA GCAGCCAGAG ACTGTGCAGC AAACGGGTGA GCTACAGGAT 
CCCAAAAGCC AGATGAGAGC CTTTAGAGAC CCTCTGCAGG ATGACACTGG ATTGTATGTC 
ACTGAGGAAG TCACCTCTTC TGAGGATAAG CGGAAGACTT ACTCTTTGGC CTTTAAACAG 
GAATTAAAAG ACGTAATTCT CTGTATCTCC CCAGTGATCA CATTCCGAGA GCCTTTCCTT 
TTAACCGAGA AGGGGATGCG ATGCTCTCCC CGAGACTATT TCGCAGAGCA GCTTTACTGG 
TCTCCTCTCC TCAATAAAGA CTTCAAAGAA ATGGAGAGCA GGCGGAAGAA GCAGCTGCTC 
AGGGATCTCT CCAGCCTCCA GGGCCTGAAC GGAAGTGTGC AGGCCAAGTC TATCCAAGTG 
CTGCCCTCAC ACGAGCTGGT GAGCGCTAGG ATTGCTGAGC ATCTGGGTGA CAGCCAGGGC 
CTGGGCCGGA TGCTGGCTGA CTACCGGGCC AGAGGAGGGA GAATTCAGCA GAATACTTCA 
GACCCTTTTG CTCATTCAAA GGATGCATCA GGTCCTTCAA GTGGCAAGTC AGGAAGCAGA 
ACTGAGGGCG ATGAAGAGAA AGGGCTGATT CCGAGTGACG CGGTATGGTC AACAAAGGTG 
GACTGTCTGA ACCCTGCCAA CCACCAGAGG CTGTGCGTGC TCTTCAGCAG CTCTTCTGCC 
CAGTCCAGCA ACGCGCCCAG CGCCTGCGTC AGTCCTTGGA TTGTAACAAT GGAATTTTAT 
GGAAAGAATG ATCTTACACT GGGAATATTT TTAGAGAGAT ACTGTTTCAG GCCTTCTTAC 
CAGTGTCCTA GCATGTTCTG TGATACCCCC ATGGTACATC ACATTCGGCG CTTTGTCCAT 
GGCCAAGGCT GTGTGCAGAT AATCCTGAAG GAGCTGGATT CTCCAGTACC TGGATATCAG 
CATACAATTC TCACCTATTC CTGGTGCAGA ATCTGCAAAC AGGTAACACC AGTTGTTGCT 
CTTTCCAATG AGTCCTGGTC CATGTCATTT GCCAAATACC TTGAACTTCG GTTTTATGGG 
CACCAGTACA CCCGTAGAGC CAATGCTGAG CCGTGTGGCC ACTCCATCCA CCATGATTAT 
CACCAGTACT TCTCCTATAA CCAGATGGTG GCATCTTTTA GTTATTCTCC CATTCGGCTT 
CTTGAAGTAT GTGTTCCACT CCCCAAAATA TTCATTAAAC GTCAGGCCCC ATTAAAAGTG 
TCCCTTCTTC AAGATCTGAA GGACTTCTTT CAAAAAGTTT CACAAGTATA TCTTGCTGTT 
GATGAAAGAC TTGCATCTTT GAAAACTGAT ACATTTAGCA AAACAAGAGA GGAAAAAATG 
GAAGATATCT TTGCACAAAA AGAGATGGAG GAAGGCGAGT TCAAGAACTG GATTGAGAAA 
ATGCAAGCGA GGCTCCTGTC TTCCTCGGTA GACACCCCTC AGCAGCTGCA GTCGGTCCTG 
GAGTCGCTCA TCGCCAAGAA GCAGAGCCTC TGTGAAGTGC TGCAGGCCTG GAATAACAGG 
TTGCAGGACC TTTTCCAGCA GGAAAAGGGT AGAAAGCGGC CTTCAGTTCC TCCAAGTCCT 
GGAAGACTGA GACAAGGGGA AGAAAGCAAG ATAAGTGCAA TGGATGCATC TCCACGGAAT 
ATTTCTCCAG GACTTCAAAA TGGAGAAAAA GAGGACCGCT TCTTGACAAC CCTCTCTAGT 
CAGGGCCCCA CCTCTGCTGC TCATCTCCAG CTGCCCAGTC CCCCTGAAGT CCTGCCTGAG 
CAGGCAGGGG GGGCGACCCC CGAGCCGGAT GCAGCCAGCA GTTCTGAAGA TGTGTTTGAT 
GGACATTCTC TGGGATCCAC AGACAGCCAG GTGAAGGAAA AATCAACCAT GAAGGCCATC 
TTTGCAAATT TGCTTCCAGG AAATAGCTAT AACCCTATTC CGTTTCCTTT TGATCCAGAT 
AAACACTACT TAATGTACGA ACACGAACGG GTGCCCATTG CCGTCTGTGA GAGGGAGCCC 
AGCTCCATCA TTGCTTTTGC TCTCAGTTGT AAAGAATACC GAAATGCCTT AGAGGAATTG 
TCCAAGGCAA CTCTGCGGAA CAGTGCCGAA GAAGGGCTCC CAGCCAACAG TGCTTCAGAC 
AGCAGACCAA AGGGCGGCAG CCCTGGGAGG CCGCCTGAGC TCGGTGGAGG CTGCCCCAAC 
CGGGCGGCCG AGGCAGAGCC GCAGCCAGCC AAAAAGGCTT CTGGGATGTT GTCCTTCTTC 
CGAGGGACAG CAGGGAAAAG CCCTGATCTC TCTTCCCAGA AGAGAGAGAC CTTACGTGGA 
GCAGATAGTG CTTACTACCA GGTCGGGCAG CCCGGCAAGG AGGGGACGGA GAGTCAAGGC 
ACTGAGCCTC CAGATGAGAT AGATGGAGGA GACGCACAAA AGAAACAGCT CACAAATCCT 
CATGTGGAAC TTCAATTTTC TGACGCTAAC GCCAAGTTTT ACTGCCGGCT CTACTATGCG 
GGAGAGTTTC ACAAGATGCG CGAGGTGATC CTGGGCAGCA GCGAGGAGGA CTTCATCCGC 
TCCCTTTCCC ACTCGGCACC CTGGCAGGCA CGCGGGGGCA AGTCCGGAGC GGCCTTCTAC 
GCCACGGAAG ATGATAGATT CATTTTGAAG CAGATGCCTC GTCTGGAAGT CCAGTCTTTC 
CTCGACTTTG CACCACACTA CTTCAATTAT ATCACAAACG CTGTGCAACA AAAGAGGCCT 
ACTGCACTGG CCAAAATTCT TGGAGTTTAC AGAATTGGTT ATAAGAATTC TCAGAACAAC 
ACTGAGAAGA AGTTAGATCT CCTTGTCATG GAAAATCTTT TCTATGGGAG AAAGATGGCA 
CAGGTTTTTG ACTTGAAGGG TTCACTTAGG AACAGAAATG TAAAAACTGA CACCGGGAAA 
GAGAGCTGTG ACGTGGTCCT GCTGGACGAG AATCTGCTGA AGATGGTCCG GGACAGCCCT 
CTGTACATCC GCTCGCACTC CAAGGCGGTG CTGCGGGCCT CCATCCGCAG CGATGCCCAC 
TTCCTGTCCA GCCACCTCAT CATCGACTAT TCTTTGCTGG TGGGGCGAGA TGATACCAGC 
AATGAGCTTG TCGTTGGGAT TATAGATTAT ATTCGAACGT TTACATGGGA CAAAAAGCTT 
GAGATGGTTG TGAAATCAAC AGGAATTTTA GGAGGACAAG GTAAAATGCC AACTGTGGTC 
TCTCCAGAGT TGTACAGGAC CCGATTTTGT GAAGCGATGG ACAAGTATTT CCTCATGGTA 
CCAGACCACT GGACAGGCTT GGGTCTCAAC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023478287.1
CDS	210..6605
Translation	MATDDKTSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPAFKSAYSSFVNLFRFNKDRAEGEQQSLTGGWPSPQLPSRTQSVRSPIPYKKQLTEELQRRPSAVLAENSLQHPQENTDTRRKTEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCRECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRMDLAVPAPLDAVWIKSYIHGPTGSASITNLSLDRSGSPLVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAVAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTDFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSPSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHVKKIPGGKKFDSMVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDLLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPGEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEEQEDDEAAQEQLSGSPLPREPDFPLECLAPDESSSLESRTVFGKSDQELKGAPKDAVSLKQQEFATAACPAGVPYALFALVPESLLPLHLGDQQDARGSEQPETVQQTGELQDPKSQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSPRDYFAEQLYWSPLLNKDFKEMESRRKKQLLRDLSSLQGLNGSVQAKSIQVLPSHELVSARIAEHLGDSQGLGRMLADYRARGGRIQQNTSDPFAHSKDASGPSSGKSGSRTEGDEEKGLIPSDAVWSTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLLSSSVDTPQQLQSVLESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQGPTSAAHLQLPSPPEVLPEQAGGATPEPDAASSSEDVFDGHSLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSASDSRPKGGSPGRPPELGGGCPNRAAEAEPQPAKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQPGKEGTESQGTEPPDEIDGGDAQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSAPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDSPLYIRSHSKAVLRASIRSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_023622519.1
Organism	Equus caballus(horse)
Definition	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023622519.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGCAGA GAACAGTTTG CAGCACCCCC AGGAGAACAC AGACACAAGA 
AGGAAAACAG AACCTACCTT TGGCGGTCAT GACCCTCGTA CAGCTGTTCA GCTTCGAAGC 
CTCAGCACAG TGTTAAAACG CCTCAAGGAA ATCATGGAGG GGAAAAGCCA GGACAGTGAC 
CTGAAGCAGT ACTGGATGCC GGACAGCCAG TGCAGAGAGT GCTACGACTG CAGCGAGAAG 
TTCACCACCT TCCGGCGCAG GCACCACTGC CGGCTCTGCG GGCAGATTTT CTGCAGCCGC 
TGCTGCAATC AAGAGATCCC TGGGAAGTTT ATGGGCTATA CAGGGGACCT CCGAGCTTGC 
ACATACTGTA GAAAAATAGC CTTAAGTTAT GCTCATTCCA CAGACAGTAA TTCCATTGGG 
GAAGACTTGA ATGCTCTTTC AGATTCTGCT TCCTCTGTGT CTGTACTTGA TCCGAGTGAA 
CCCCGAACAC CTGTTGGGAG TAGGAAAGCC AGCCGGAACA TCTTTTTAGA GGATGATTTT 
GCCTGGCAAA GTTTGATTCA TCCAGACTCC TCAAATACTG CTCTTTCAAC AAGACTTGTA 
TCTGTTCAAG AGGACGCTGG GAAATCTCCT GCTCGAAATA GAATGGACTT GGCAGTGCCT 
GCTCCATTGG ATGCTGTGTG GATTAAATCA TACATACATG GCCCTACTGG GTCAGCCAGC 
ATTACCAACC TCTCACTGGA TCGATCTGGT TCTCCCCTGG TACCTTCATA TGAGACATCT 
GTCAGTCCCC AGGCCAACCG AACATACGTG AGGACAGAGA CCACAGAGGA TGAACGCAAA 
ATTCTTCTGG ACAGCGTCCA GTTAAAAGAC CTGTGGAAGA AAATCTGCCA TCACAGTAGT 
GGAATGGAGT TTCAGGATCA CCGCTACTGG CTGAGGACAC ATCCCAACTG CATCGTAGGG 
AAGGAGTTAG TCAACTGGCT AATCCGAAAT GGGCACATTG CCACAAGGGC ACAAGCTGTA 
GCAATTGGAC AAGCGATGGT GGACGGACGG TGGCTGGACT GCGTGAGCCA CCATGACCAG 
CTCTTCAGGG ATGAGTATGC GCTCTACAGA CCCCTGCAGA GTACAGACTT TTCTGAGACC 
CCTTCTCCGG ACAGTGACTC CGTGAACTCT GTGGAAGGAC ACTCGGAGCC ATCATGGTTT 
AAAGACATAA AGTTTGATGA CAGCGACACA GAGCAGATTG CTGAAGAAGG TGACGATAAT 
TTGACTAACT CTGCCAGTCC TAGCAAGCGC ACGTCAGTCA GCAGTTTCCA GTCCACAGTG 
GACAGTGACT CAGCCGCCTC CATCAGCCTG AACGTGGAGT TGGACAACGT GAACTTCCAC 
ATCAAGAAGC CCTCCAAGTA CCCACACGTG CCCCCTCACC CTGCTGACCA AAAAGAGTAT 
TTGATTTCTG ACAACGGAGG ACAACAACTC TCAATAAGTG ACGCCTTCAT CAAAGAATCC 
TTATTTAATC GTCGAGTTGA GGAGAAATCC AAAGAACTGC CCTTTACGCC TTTGGGCTGG 
CATCATAACA ATCTGGAGCT GCTGCGGGAG GAGAATGGAG AGAAGCAGGC CATGGAGAGG 
TTGCTTTCAG CTAATCATAA CCACATGATG GCACTGCTCC AACAGTTGCT CCATAGTGAG 
TCATTGTCAC CATCTTGGAG GGACATCATT GTATCCCTGG TCTGCCAGGT TGTTCAGACA 
GTCCGACCTG ACGTCAAGAA CCGGGATGAC GACATGGATA TCCGTCAGTT TGTCCACGTC 
AAAAAAATCC CAGGTGGAAA GAAGTTTGAT TCTATGGTTG TCAATGGGTT CGTTTGTACC 
AAGAATATTG CACATAAAAA GATGAATTCT TGTATTAAAA ACCCCAAAAT TCTTCTGTTG 
AAGTGTTCCA TTGAGTATCT CTACAGAGAA GAAACTAAAT TTACTTGCAT TGACCCTATC 
GTGCTTCAGG AAAGGGAATT CTTGAAGAAT TATGTTCAGC GAATAGTGGA TGTTCGACCC 
ACATTGGTTC TAGTTGAGAA AACGGTGTCT CGGATTGCCC AGGACCTGTT GCTGGAACAC 
GGCATCACTT TGGTCATTAA TGTCAAATCA CAAGTTTTAG AACGAATCAG TCGGATGACC 
CAAGGTGATT TAGTGATGTC GATGGACCAG TTGCTCACAA AGCCCCACCT GGGCACCTGT 
CACAAATTTT ACATGCAGAT GTTTCAGCTA CCTGGTGAAC AAACCAAAAC TCTGATGTTT 
TTTGAAGGCT GTCCACAGCA CCTGGGCTGT ACAATCAAGC TTAGAGGAGG CTCCGATTAT 
GAGCTGGCTC GAGTTAAGGA GATCCTCATA TTTATGATCT GTGTAGCATA TCATTCTCAA 
CTAGAAATCT CCTTCCTCAT GGATGAATTT GCCATGCCTC CTACATTAAC GCAAAACCCT 
TCCTTCCACT CTCTGATTGA GGAACAGGAG GATGACGAGG CTGCTCAAGA ACAGCTCAGC 
GGAAGTCCCC TCCCCCGGGA ACCTGACTTC CCTCTGGAGT GTCTGGCCCC TGATGAGAGC 
AGTTCACTGG AATCAAGGAC TGTGTTTGGG AAGAGTGACC AGGAACTTAA AGGTGCTCCA 
AAGGATGCTG TCTCGTTGAA GCAGCAGGAG TTCGCCACAG CAGCCTGCCC GGCCGGCGTC 
CCCTATGCTC TCTTTGCATT GGTCCCAGAA TCCTTGTTGC CACTGCATCT GGGTGACCAA 
CAGGATGCCA GGGGCAGCGA GCAGCCAGAG ACTGTGCAGC AAACGGGTGA GCTACAGGAT 
CCCAAAAGCC AGATGAGAGC CTTTAGAGAC CCTCTGCAGG ATGACACTGG ATTGTATGTC 
ACTGAGGAAG TCACCTCTTC TGAGGATAAG CGGAAGACTT ACTCTTTGGC CTTTAAACAG 
GAATTAAAAG ACGTAATTCT CTGTATCTCC CCAGTGATCA CATTCCGAGA GCCTTTCCTT 
TTAACCGAGA AGGGGATGCG ATGCTCTCCC CGAGACTATT TCGCAGAGCA GCTTTACTGG 
TCTCCTCTCC TCAATAAAGA CTTCAAAGAA ATGGAGAGCA GGCGGAAGAA GCAGCTGCTC 
AGGGATCTCT CCAGCCTCCA GGGCCTGAAC GGAAGTGTGC AGGCCAAGTC TATCCAAGTG 
CTGCCCTCAC ACGAGCTGGT GAGCGCTAGG ATTGCTGAGC ATCTGGGTGA CAGCCAGGGC 
CTGGGCCGGA TGCTGGCTGA CTACCGGGCC AGAGGAGGGA GAATTCAGCA GAATACTTCA 
GACCCTTTTG CTCATTCAAA GGATGCATCA GGTCCTTCAA GTGGCAAGTC AGGAAGCAGA 
ACTGAGGGCG ATGAAGAGAA AGGGCTGATT CCGAGTGACG CGGTATGGTC AACAAAGGTG 
GACTGTCTGA ACCCTGCCAA CCACCAGAGG CTGTGCGTGC TCTTCAGCAG CTCTTCTGCC 
CAGTCCAGCA ACGCGCCCAG CGCCTGCGTC AGTCCTTGGA TTGTAACAAT GGAATTTTAT 
GGAAAGAATG ATCTTACACT GGGAATATTT TTAGAGAGAT ACTGTTTCAG GCCTTCTTAC 
CAGTGTCCTA GCATGTTCTG TGATACCCCC ATGGTACATC ACATTCGGCG CTTTGTCCAT 
GGCCAAGGCT GTGTGCAGAT AATCCTGAAG GAGCTGGATT CTCCAGTACC TGGATATCAG 
CATACAATTC TCACCTATTC CTGGTGCAGA ATCTGCAAAC AGGTAACACC AGTTGTTGCT 
CTTTCCAATG AGTCCTGGTC CATGTCATTT GCCAAATACC TTGAACTTCG GTTTTATGGG 
CACCAGTACA CCCGTAGAGC CAATGCTGAG CCGTGTGGCC ACTCCATCCA CCATGATTAT 
CACCAGTACT TCTCCTATAA CCAGATGGTG GCATCTTTTA GTTATTCTCC CATTCGGCTT 
CTTGAAGTAT GTGTTCCACT CCCCAAAATA TTCATTAAAC GTCAGGCCCC ATTAAAAGTG 
TCCCTTCTTC AAGATCTGAA GGACTTCTTT CAAAAAGTTT CACAAGTATA TCTTGCTGTT 
GATGAAAGAC TTGCATCTTT GAAAACTGAT ACATTTAGCA AAACAAGAGA GGAAAAAATG 
GAAGATATCT TTGCACAAAA AGAGATGGAG GAAGGCGAGT TCAAGAACTG GATTGAGAAA 
ATGCAAGCGA GGCTCCTGTC TTCCTCGGTA GACACCCCTC AGCAGCTGCA GTCGGTCCTG 
GAGTCGCTCA TCGCCAAGAA GCAGAGCCTC TGTGAAGTGC TGCAGGCCTG GAATAACAGG 
TTGCAGGACC TTTTCCAGCA GGAAAAGGGT AGAAAGCGGC CTTCAGTTCC TCCAAGTCCT 
GGAAGACTGA GACAAGGGGA AGAAAGCAAG ATAAGTGCAA TGGATGCATC TCCACGGAAT 
ATTTCTCCAG GACTTCAAAA TGGAGAAAAA GAGGACCGCT TCTTGACAAC CCTCTCTAGT 
CAGGGCCCCA CCTCTGCTGC TCATCTCCAG CTGCCCAGTC CCCCTGAAGT CCTGCCTGAG 
CAGGCAGGGG GGGCGACCCC CGAGCCGGAT GCAGCCAGCA GTTCTGAAGA TGTGTTTGAT 
GGACATTCTC TGGGATCCAC AGACAGCCAG GTGAAGGAAA AATCAACCAT GAAGGCCATC 
TTTGCAAATT TGCTTCCAGG AAATAGCTAT AACCCTATTC CGTTTCCTTT TGATCCAGAT 
AAACACTACT TAATGTACGA ACACGAACGG GTGCCCATTG CCGTCTGTGA GAGGGAGCCC 
AGCTCCATCA TTGCTTTTGC TCTCAGTTGT AAAGAATACC GAAATGCCTT AGAGGAATTG 
TCCAAGGCAA CTCTGCGGAA CAGTGCCGAA GAAGGGCTCC CAGCCAACAG TGCTTCAGAC 
AGCAGACCAA AGGGCGGCAG CCCTGGGAGG CCGCCTGAGC TCGGTGGAGG CTGCCCCAAC 
CGGGCGGCCG AGGCAGAGCC GCAGCCAGCC AAAAAGGCTT CTGGGATGTT GTCCTTCTTC 
CGAGGGACAG CAGGGAAAAG CCCTGATCTC TCTTCCCAGA AGAGAGAGAC CTTACGTGGA 
GCAGATAGTG CTTACTACCA GGTCGGGCAG CCCGGCAAGG AGGGGACGGA GAGTCAAGGC 
ACTGAGCCTC CAGATGAGAT AGATGGAGGA GACGCACAAA AGAAACAGCT CACAAATCCT 
CATGTGGAAC TTCAATTTTC TGACGCTAAC GCCAAGTTTT ACTGCCGGCT CTACTATGCG 
GGAGAGTTTC ACAAGATGCG CGAGGTGATC CTGGGCAGCA GCGAGGAGGA CTTCATCCGC 
TCCCTTTCCC ACTCGGCACC CTGGCAGGCA CGCGGGGGCA AGTCCGGAGC GGCCTTCTAC 
GCCACGGAAG ATGATAGATT CATTTTGAAG CAGATGCCTC GTCTGGAAGT CCAGTCTTTC 
CTCGACTTTG CACCACACTA CTTCAATTAT ATCACAAACG CTGTGCAACA AAAGAGGCCT 
ACTGCACTGG CCAAAATTCT TGGAGTTTAC AGAATTGGTT ATAAGAATTC TCAGAACAAC 
ACTGAGAAGA AGTTAGATCT CCTTGTCATG GAAAATCTTT TCTATGGGAG AAAGATGGCA 
CAGGTTTTTG ACTTGAAGGG TTCACTTAGG AACAGAAATG TAAAAACTGA CACCGGGAAA 
GAGAGCTGTG ACGTGGTCCT GCTGGACGAG AATCTGCTGA AGATGGTCCG GGACAGCCCT 
CTGTACATCC GCTCGCACTC CAAGGCGGTG CTGCGGGCCT CCATCCGCAG CGATGCCCAC 
TTCCTGTCCA GCCACCTCAT CATCGACTAT TCTTTGCTGG TGGGGCGAGA TGATACCAGC 
AATGAGCTTG TCGTTGGGAT TATAGATTAT ATTCGAACGT TTACATGGGA CAAAAAGCTT 
GAGATGGTTG TGAAATCAAC AGGAATTTTA GGAGGACAAG GTAAAATGCC AACTGTGGTC 
TCTCCAGAGT TGTACAGGAC CCGATTTTGT GAAGCGATGG ACAAGTATTT CCTCATGGTA 
CCAGACCACT GGACAGGCTT GGGTCTCAAC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_023622517.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023478285.1 Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X1, mRNA.

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CloneID	OHb52469
Clone ID Related Accession (Same CDS sequence)	XM_023622519.1 , XM_023622517.1 , XM_023622518.1 , XM_023622520.1
Accession Version	XM_023622517.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGCAGA GAACAGTTTG CAGCACCCCC AGGAGAACAC AGACACAAGA 
AGGAAAACAG AACCTACCTT TGGCGGTCAT GACCCTCGTA CAGCTGTTCA GCTTCGAAGC 
CTCAGCACAG TGTTAAAACG CCTCAAGGAA ATCATGGAGG GGAAAAGCCA GGACAGTGAC 
CTGAAGCAGT ACTGGATGCC GGACAGCCAG TGCAGAGAGT GCTACGACTG CAGCGAGAAG 
TTCACCACCT TCCGGCGCAG GCACCACTGC CGGCTCTGCG GGCAGATTTT CTGCAGCCGC 
TGCTGCAATC AAGAGATCCC TGGGAAGTTT ATGGGCTATA CAGGGGACCT CCGAGCTTGC 
ACATACTGTA GAAAAATAGC CTTAAGTTAT GCTCATTCCA CAGACAGTAA TTCCATTGGG 
GAAGACTTGA ATGCTCTTTC AGATTCTGCT TCCTCTGTGT CTGTACTTGA TCCGAGTGAA 
CCCCGAACAC CTGTTGGGAG TAGGAAAGCC AGCCGGAACA TCTTTTTAGA GGATGATTTT 
GCCTGGCAAA GTTTGATTCA TCCAGACTCC TCAAATACTG CTCTTTCAAC AAGACTTGTA 
TCTGTTCAAG AGGACGCTGG GAAATCTCCT GCTCGAAATA GAATGGACTT GGCAGTGCCT 
GCTCCATTGG ATGCTGTGTG GATTAAATCA TACATACATG GCCCTACTGG GTCAGCCAGC 
ATTACCAACC TCTCACTGGA TCGATCTGGT TCTCCCCTGG TACCTTCATA TGAGACATCT 
GTCAGTCCCC AGGCCAACCG AACATACGTG AGGACAGAGA CCACAGAGGA TGAACGCAAA 
ATTCTTCTGG ACAGCGTCCA GTTAAAAGAC CTGTGGAAGA AAATCTGCCA TCACAGTAGT 
GGAATGGAGT TTCAGGATCA CCGCTACTGG CTGAGGACAC ATCCCAACTG CATCGTAGGG 
AAGGAGTTAG TCAACTGGCT AATCCGAAAT GGGCACATTG CCACAAGGGC ACAAGCTGTA 
GCAATTGGAC AAGCGATGGT GGACGGACGG TGGCTGGACT GCGTGAGCCA CCATGACCAG 
CTCTTCAGGG ATGAGTATGC GCTCTACAGA CCCCTGCAGA GTACAGACTT TTCTGAGACC 
CCTTCTCCGG ACAGTGACTC CGTGAACTCT GTGGAAGGAC ACTCGGAGCC ATCATGGTTT 
AAAGACATAA AGTTTGATGA CAGCGACACA GAGCAGATTG CTGAAGAAGG TGACGATAAT 
TTGACTAACT CTGCCAGTCC TAGCAAGCGC ACGTCAGTCA GCAGTTTCCA GTCCACAGTG 
GACAGTGACT CAGCCGCCTC CATCAGCCTG AACGTGGAGT TGGACAACGT GAACTTCCAC 
ATCAAGAAGC CCTCCAAGTA CCCACACGTG CCCCCTCACC CTGCTGACCA AAAAGAGTAT 
TTGATTTCTG ACAACGGAGG ACAACAACTC TCAATAAGTG ACGCCTTCAT CAAAGAATCC 
TTATTTAATC GTCGAGTTGA GGAGAAATCC AAAGAACTGC CCTTTACGCC TTTGGGCTGG 
CATCATAACA ATCTGGAGCT GCTGCGGGAG GAGAATGGAG AGAAGCAGGC CATGGAGAGG 
TTGCTTTCAG CTAATCATAA CCACATGATG GCACTGCTCC AACAGTTGCT CCATAGTGAG 
TCATTGTCAC CATCTTGGAG GGACATCATT GTATCCCTGG TCTGCCAGGT TGTTCAGACA 
GTCCGACCTG ACGTCAAGAA CCGGGATGAC GACATGGATA TCCGTCAGTT TGTCCACGTC 
AAAAAAATCC CAGGTGGAAA GAAGTTTGAT TCTATGGTTG TCAATGGGTT CGTTTGTACC 
AAGAATATTG CACATAAAAA GATGAATTCT TGTATTAAAA ACCCCAAAAT TCTTCTGTTG 
AAGTGTTCCA TTGAGTATCT CTACAGAGAA GAAACTAAAT TTACTTGCAT TGACCCTATC 
GTGCTTCAGG AAAGGGAATT CTTGAAGAAT TATGTTCAGC GAATAGTGGA TGTTCGACCC 
ACATTGGTTC TAGTTGAGAA AACGGTGTCT CGGATTGCCC AGGACCTGTT GCTGGAACAC 
GGCATCACTT TGGTCATTAA TGTCAAATCA CAAGTTTTAG AACGAATCAG TCGGATGACC 
CAAGGTGATT TAGTGATGTC GATGGACCAG TTGCTCACAA AGCCCCACCT GGGCACCTGT 
CACAAATTTT ACATGCAGAT GTTTCAGCTA CCTGGTGAAC AAACCAAAAC TCTGATGTTT 
TTTGAAGGCT GTCCACAGCA CCTGGGCTGT ACAATCAAGC TTAGAGGAGG CTCCGATTAT 
GAGCTGGCTC GAGTTAAGGA GATCCTCATA TTTATGATCT GTGTAGCATA TCATTCTCAA 
CTAGAAATCT CCTTCCTCAT GGATGAATTT GCCATGCCTC CTACATTAAC GCAAAACCCT 
TCCTTCCACT CTCTGATTGA GGAACAGGAG GATGACGAGG CTGCTCAAGA ACAGCTCAGC 
GGAAGTCCCC TCCCCCGGGA ACCTGACTTC CCTCTGGAGT GTCTGGCCCC TGATGAGAGC 
AGTTCACTGG AATCAAGGAC TGTGTTTGGG AAGAGTGACC AGGAACTTAA AGGTGCTCCA 
AAGGATGCTG TCTCGTTGAA GCAGCAGGAG TTCGCCACAG CAGCCTGCCC GGCCGGCGTC 
CCCTATGCTC TCTTTGCATT GGTCCCAGAA TCCTTGTTGC CACTGCATCT GGGTGACCAA 
CAGGATGCCA GGGGCAGCGA GCAGCCAGAG ACTGTGCAGC AAACGGGTGA GCTACAGGAT 
CCCAAAAGCC AGATGAGAGC CTTTAGAGAC CCTCTGCAGG ATGACACTGG ATTGTATGTC 
ACTGAGGAAG TCACCTCTTC TGAGGATAAG CGGAAGACTT ACTCTTTGGC CTTTAAACAG 
GAATTAAAAG ACGTAATTCT CTGTATCTCC CCAGTGATCA CATTCCGAGA GCCTTTCCTT 
TTAACCGAGA AGGGGATGCG ATGCTCTCCC CGAGACTATT TCGCAGAGCA GCTTTACTGG 
TCTCCTCTCC TCAATAAAGA CTTCAAAGAA ATGGAGAGCA GGCGGAAGAA GCAGCTGCTC 
AGGGATCTCT CCAGCCTCCA GGGCCTGAAC GGAAGTGTGC AGGCCAAGTC TATCCAAGTG 
CTGCCCTCAC ACGAGCTGGT GAGCGCTAGG ATTGCTGAGC ATCTGGGTGA CAGCCAGGGC 
CTGGGCCGGA TGCTGGCTGA CTACCGGGCC AGAGGAGGGA GAATTCAGCA GAATACTTCA 
GACCCTTTTG CTCATTCAAA GGATGCATCA GGTCCTTCAA GTGGCAAGTC AGGAAGCAGA 
ACTGAGGGCG ATGAAGAGAA AGGGCTGATT CCGAGTGACG CGGTATGGTC AACAAAGGTG 
GACTGTCTGA ACCCTGCCAA CCACCAGAGG CTGTGCGTGC TCTTCAGCAG CTCTTCTGCC 
CAGTCCAGCA ACGCGCCCAG CGCCTGCGTC AGTCCTTGGA TTGTAACAAT GGAATTTTAT 
GGAAAGAATG ATCTTACACT GGGAATATTT TTAGAGAGAT ACTGTTTCAG GCCTTCTTAC 
CAGTGTCCTA GCATGTTCTG TGATACCCCC ATGGTACATC ACATTCGGCG CTTTGTCCAT 
GGCCAAGGCT GTGTGCAGAT AATCCTGAAG GAGCTGGATT CTCCAGTACC TGGATATCAG 
CATACAATTC TCACCTATTC CTGGTGCAGA ATCTGCAAAC AGGTAACACC AGTTGTTGCT 
CTTTCCAATG AGTCCTGGTC CATGTCATTT GCCAAATACC TTGAACTTCG GTTTTATGGG 
CACCAGTACA CCCGTAGAGC CAATGCTGAG CCGTGTGGCC ACTCCATCCA CCATGATTAT 
CACCAGTACT TCTCCTATAA CCAGATGGTG GCATCTTTTA GTTATTCTCC CATTCGGCTT 
CTTGAAGTAT GTGTTCCACT CCCCAAAATA TTCATTAAAC GTCAGGCCCC ATTAAAAGTG 
TCCCTTCTTC AAGATCTGAA GGACTTCTTT CAAAAAGTTT CACAAGTATA TCTTGCTGTT 
GATGAAAGAC TTGCATCTTT GAAAACTGAT ACATTTAGCA AAACAAGAGA GGAAAAAATG 
GAAGATATCT TTGCACAAAA AGAGATGGAG GAAGGCGAGT TCAAGAACTG GATTGAGAAA 
ATGCAAGCGA GGCTCCTGTC TTCCTCGGTA GACACCCCTC AGCAGCTGCA GTCGGTCCTG 
GAGTCGCTCA TCGCCAAGAA GCAGAGCCTC TGTGAAGTGC TGCAGGCCTG GAATAACAGG 
TTGCAGGACC TTTTCCAGCA GGAAAAGGGT AGAAAGCGGC CTTCAGTTCC TCCAAGTCCT 
GGAAGACTGA GACAAGGGGA AGAAAGCAAG ATAAGTGCAA TGGATGCATC TCCACGGAAT 
ATTTCTCCAG GACTTCAAAA TGGAGAAAAA GAGGACCGCT TCTTGACAAC CCTCTCTAGT 
CAGGGCCCCA CCTCTGCTGC TCATCTCCAG CTGCCCAGTC CCCCTGAAGT CCTGCCTGAG 
CAGGCAGGGG GGGCGACCCC CGAGCCGGAT GCAGCCAGCA GTTCTGAAGA TGTGTTTGAT 
GGACATTCTC TGGGATCCAC AGACAGCCAG GTGAAGGAAA AATCAACCAT GAAGGCCATC 
TTTGCAAATT TGCTTCCAGG AAATAGCTAT AACCCTATTC CGTTTCCTTT TGATCCAGAT 
AAACACTACT TAATGTACGA ACACGAACGG GTGCCCATTG CCGTCTGTGA GAGGGAGCCC 
AGCTCCATCA TTGCTTTTGC TCTCAGTTGT AAAGAATACC GAAATGCCTT AGAGGAATTG 
TCCAAGGCAA CTCTGCGGAA CAGTGCCGAA GAAGGGCTCC CAGCCAACAG TGCTTCAGAC 
AGCAGACCAA AGGGCGGCAG CCCTGGGAGG CCGCCTGAGC TCGGTGGAGG CTGCCCCAAC 
CGGGCGGCCG AGGCAGAGCC GCAGCCAGCC AAAAAGGCTT CTGGGATGTT GTCCTTCTTC 
CGAGGGACAG CAGGGAAAAG CCCTGATCTC TCTTCCCAGA AGAGAGAGAC CTTACGTGGA 
GCAGATAGTG CTTACTACCA GGTCGGGCAG CCCGGCAAGG AGGGGACGGA GAGTCAAGGC 
ACTGAGCCTC CAGATGAGAT AGATGGAGGA GACGCACAAA AGAAACAGCT CACAAATCCT 
CATGTGGAAC TTCAATTTTC TGACGCTAAC GCCAAGTTTT ACTGCCGGCT CTACTATGCG 
GGAGAGTTTC ACAAGATGCG CGAGGTGATC CTGGGCAGCA GCGAGGAGGA CTTCATCCGC 
TCCCTTTCCC ACTCGGCACC CTGGCAGGCA CGCGGGGGCA AGTCCGGAGC GGCCTTCTAC 
GCCACGGAAG ATGATAGATT CATTTTGAAG CAGATGCCTC GTCTGGAAGT CCAGTCTTTC 
CTCGACTTTG CACCACACTA CTTCAATTAT ATCACAAACG CTGTGCAACA AAAGAGGCCT 
ACTGCACTGG CCAAAATTCT TGGAGTTTAC AGAATTGGTT ATAAGAATTC TCAGAACAAC 
ACTGAGAAGA AGTTAGATCT CCTTGTCATG GAAAATCTTT TCTATGGGAG AAAGATGGCA 
CAGGTTTTTG ACTTGAAGGG TTCACTTAGG AACAGAAATG TAAAAACTGA CACCGGGAAA 
GAGAGCTGTG ACGTGGTCCT GCTGGACGAG AATCTGCTGA AGATGGTCCG GGACAGCCCT 
CTGTACATCC GCTCGCACTC CAAGGCGGTG CTGCGGGCCT CCATCCGCAG CGATGCCCAC 
TTCCTGTCCA GCCACCTCAT CATCGACTAT TCTTTGCTGG TGGGGCGAGA TGATACCAGC 
AATGAGCTTG TCGTTGGGAT TATAGATTAT ATTCGAACGT TTACATGGGA CAAAAAGCTT 
GAGATGGTTG TGAAATCAAC AGGAATTTTA GGAGGACAAG GTAAAATGCC AACTGTGGTC 
TCTCCAGAGT TGTACAGGAC CCGATTTTGT GAAGCGATGG ACAAGTATTT CCTCATGGTA 
CCAGACCACT GGACAGGCTT GGGTCTCAAC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023478285.1
CDS	114..6509
Translation	MATDDKTSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPAFKSAYSSFVNLFRFNKDRAEGEQQSLTGGWPSPQLPSRTQSVRSPIPYKKQLTEELQRRPSAVLAENSLQHPQENTDTRRKTEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCRECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRMDLAVPAPLDAVWIKSYIHGPTGSASITNLSLDRSGSPLVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAVAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTDFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSPSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHVKKIPGGKKFDSMVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDLLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPGEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEEQEDDEAAQEQLSGSPLPREPDFPLECLAPDESSSLESRTVFGKSDQELKGAPKDAVSLKQQEFATAACPAGVPYALFALVPESLLPLHLGDQQDARGSEQPETVQQTGELQDPKSQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSPRDYFAEQLYWSPLLNKDFKEMESRRKKQLLRDLSSLQGLNGSVQAKSIQVLPSHELVSARIAEHLGDSQGLGRMLADYRARGGRIQQNTSDPFAHSKDASGPSSGKSGSRTEGDEEKGLIPSDAVWSTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLLSSSVDTPQQLQSVLESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQGPTSAAHLQLPSPPEVLPEQAGGATPEPDAASSSEDVFDGHSLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSASDSRPKGGSPGRPPELGGGCPNRAAEAEPQPAKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQPGKEGTESQGTEPPDEIDGGDAQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSAPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDSPLYIRSHSKAVLRASIRSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_023622517.1
Organism	Equus caballus(horse)
Definition	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023622517.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGCAGA GAACAGTTTG CAGCACCCCC AGGAGAACAC AGACACAAGA 
AGGAAAACAG AACCTACCTT TGGCGGTCAT GACCCTCGTA CAGCTGTTCA GCTTCGAAGC 
CTCAGCACAG TGTTAAAACG CCTCAAGGAA ATCATGGAGG GGAAAAGCCA GGACAGTGAC 
CTGAAGCAGT ACTGGATGCC GGACAGCCAG TGCAGAGAGT GCTACGACTG CAGCGAGAAG 
TTCACCACCT TCCGGCGCAG GCACCACTGC CGGCTCTGCG GGCAGATTTT CTGCAGCCGC 
TGCTGCAATC AAGAGATCCC TGGGAAGTTT ATGGGCTATA CAGGGGACCT CCGAGCTTGC 
ACATACTGTA GAAAAATAGC CTTAAGTTAT GCTCATTCCA CAGACAGTAA TTCCATTGGG 
GAAGACTTGA ATGCTCTTTC AGATTCTGCT TCCTCTGTGT CTGTACTTGA TCCGAGTGAA 
CCCCGAACAC CTGTTGGGAG TAGGAAAGCC AGCCGGAACA TCTTTTTAGA GGATGATTTT 
GCCTGGCAAA GTTTGATTCA TCCAGACTCC TCAAATACTG CTCTTTCAAC AAGACTTGTA 
TCTGTTCAAG AGGACGCTGG GAAATCTCCT GCTCGAAATA GAATGGACTT GGCAGTGCCT 
GCTCCATTGG ATGCTGTGTG GATTAAATCA TACATACATG GCCCTACTGG GTCAGCCAGC 
ATTACCAACC TCTCACTGGA TCGATCTGGT TCTCCCCTGG TACCTTCATA TGAGACATCT 
GTCAGTCCCC AGGCCAACCG AACATACGTG AGGACAGAGA CCACAGAGGA TGAACGCAAA 
ATTCTTCTGG ACAGCGTCCA GTTAAAAGAC CTGTGGAAGA AAATCTGCCA TCACAGTAGT 
GGAATGGAGT TTCAGGATCA CCGCTACTGG CTGAGGACAC ATCCCAACTG CATCGTAGGG 
AAGGAGTTAG TCAACTGGCT AATCCGAAAT GGGCACATTG CCACAAGGGC ACAAGCTGTA 
GCAATTGGAC AAGCGATGGT GGACGGACGG TGGCTGGACT GCGTGAGCCA CCATGACCAG 
CTCTTCAGGG ATGAGTATGC GCTCTACAGA CCCCTGCAGA GTACAGACTT TTCTGAGACC 
CCTTCTCCGG ACAGTGACTC CGTGAACTCT GTGGAAGGAC ACTCGGAGCC ATCATGGTTT 
AAAGACATAA AGTTTGATGA CAGCGACACA GAGCAGATTG CTGAAGAAGG TGACGATAAT 
TTGACTAACT CTGCCAGTCC TAGCAAGCGC ACGTCAGTCA GCAGTTTCCA GTCCACAGTG 
GACAGTGACT CAGCCGCCTC CATCAGCCTG AACGTGGAGT TGGACAACGT GAACTTCCAC 
ATCAAGAAGC CCTCCAAGTA CCCACACGTG CCCCCTCACC CTGCTGACCA AAAAGAGTAT 
TTGATTTCTG ACAACGGAGG ACAACAACTC TCAATAAGTG ACGCCTTCAT CAAAGAATCC 
TTATTTAATC GTCGAGTTGA GGAGAAATCC AAAGAACTGC CCTTTACGCC TTTGGGCTGG 
CATCATAACA ATCTGGAGCT GCTGCGGGAG GAGAATGGAG AGAAGCAGGC CATGGAGAGG 
TTGCTTTCAG CTAATCATAA CCACATGATG GCACTGCTCC AACAGTTGCT CCATAGTGAG 
TCATTGTCAC CATCTTGGAG GGACATCATT GTATCCCTGG TCTGCCAGGT TGTTCAGACA 
GTCCGACCTG ACGTCAAGAA CCGGGATGAC GACATGGATA TCCGTCAGTT TGTCCACGTC 
AAAAAAATCC CAGGTGGAAA GAAGTTTGAT TCTATGGTTG TCAATGGGTT CGTTTGTACC 
AAGAATATTG CACATAAAAA GATGAATTCT TGTATTAAAA ACCCCAAAAT TCTTCTGTTG 
AAGTGTTCCA TTGAGTATCT CTACAGAGAA GAAACTAAAT TTACTTGCAT TGACCCTATC 
GTGCTTCAGG AAAGGGAATT CTTGAAGAAT TATGTTCAGC GAATAGTGGA TGTTCGACCC 
ACATTGGTTC TAGTTGAGAA AACGGTGTCT CGGATTGCCC AGGACCTGTT GCTGGAACAC 
GGCATCACTT TGGTCATTAA TGTCAAATCA CAAGTTTTAG AACGAATCAG TCGGATGACC 
CAAGGTGATT TAGTGATGTC GATGGACCAG TTGCTCACAA AGCCCCACCT GGGCACCTGT 
CACAAATTTT ACATGCAGAT GTTTCAGCTA CCTGGTGAAC AAACCAAAAC TCTGATGTTT 
TTTGAAGGCT GTCCACAGCA CCTGGGCTGT ACAATCAAGC TTAGAGGAGG CTCCGATTAT 
GAGCTGGCTC GAGTTAAGGA GATCCTCATA TTTATGATCT GTGTAGCATA TCATTCTCAA 
CTAGAAATCT CCTTCCTCAT GGATGAATTT GCCATGCCTC CTACATTAAC GCAAAACCCT 
TCCTTCCACT CTCTGATTGA GGAACAGGAG GATGACGAGG CTGCTCAAGA ACAGCTCAGC 
GGAAGTCCCC TCCCCCGGGA ACCTGACTTC CCTCTGGAGT GTCTGGCCCC TGATGAGAGC 
AGTTCACTGG AATCAAGGAC TGTGTTTGGG AAGAGTGACC AGGAACTTAA AGGTGCTCCA 
AAGGATGCTG TCTCGTTGAA GCAGCAGGAG TTCGCCACAG CAGCCTGCCC GGCCGGCGTC 
CCCTATGCTC TCTTTGCATT GGTCCCAGAA TCCTTGTTGC CACTGCATCT GGGTGACCAA 
CAGGATGCCA GGGGCAGCGA GCAGCCAGAG ACTGTGCAGC AAACGGGTGA GCTACAGGAT 
CCCAAAAGCC AGATGAGAGC CTTTAGAGAC CCTCTGCAGG ATGACACTGG ATTGTATGTC 
ACTGAGGAAG TCACCTCTTC TGAGGATAAG CGGAAGACTT ACTCTTTGGC CTTTAAACAG 
GAATTAAAAG ACGTAATTCT CTGTATCTCC CCAGTGATCA CATTCCGAGA GCCTTTCCTT 
TTAACCGAGA AGGGGATGCG ATGCTCTCCC CGAGACTATT TCGCAGAGCA GCTTTACTGG 
TCTCCTCTCC TCAATAAAGA CTTCAAAGAA ATGGAGAGCA GGCGGAAGAA GCAGCTGCTC 
AGGGATCTCT CCAGCCTCCA GGGCCTGAAC GGAAGTGTGC AGGCCAAGTC TATCCAAGTG 
CTGCCCTCAC ACGAGCTGGT GAGCGCTAGG ATTGCTGAGC ATCTGGGTGA CAGCCAGGGC 
CTGGGCCGGA TGCTGGCTGA CTACCGGGCC AGAGGAGGGA GAATTCAGCA GAATACTTCA 
GACCCTTTTG CTCATTCAAA GGATGCATCA GGTCCTTCAA GTGGCAAGTC AGGAAGCAGA 
ACTGAGGGCG ATGAAGAGAA AGGGCTGATT CCGAGTGACG CGGTATGGTC AACAAAGGTG 
GACTGTCTGA ACCCTGCCAA CCACCAGAGG CTGTGCGTGC TCTTCAGCAG CTCTTCTGCC 
CAGTCCAGCA ACGCGCCCAG CGCCTGCGTC AGTCCTTGGA TTGTAACAAT GGAATTTTAT 
GGAAAGAATG ATCTTACACT GGGAATATTT TTAGAGAGAT ACTGTTTCAG GCCTTCTTAC 
CAGTGTCCTA GCATGTTCTG TGATACCCCC ATGGTACATC ACATTCGGCG CTTTGTCCAT 
GGCCAAGGCT GTGTGCAGAT AATCCTGAAG GAGCTGGATT CTCCAGTACC TGGATATCAG 
CATACAATTC TCACCTATTC CTGGTGCAGA ATCTGCAAAC AGGTAACACC AGTTGTTGCT 
CTTTCCAATG AGTCCTGGTC CATGTCATTT GCCAAATACC TTGAACTTCG GTTTTATGGG 
CACCAGTACA CCCGTAGAGC CAATGCTGAG CCGTGTGGCC ACTCCATCCA CCATGATTAT 
CACCAGTACT TCTCCTATAA CCAGATGGTG GCATCTTTTA GTTATTCTCC CATTCGGCTT 
CTTGAAGTAT GTGTTCCACT CCCCAAAATA TTCATTAAAC GTCAGGCCCC ATTAAAAGTG 
TCCCTTCTTC AAGATCTGAA GGACTTCTTT CAAAAAGTTT CACAAGTATA TCTTGCTGTT 
GATGAAAGAC TTGCATCTTT GAAAACTGAT ACATTTAGCA AAACAAGAGA GGAAAAAATG 
GAAGATATCT TTGCACAAAA AGAGATGGAG GAAGGCGAGT TCAAGAACTG GATTGAGAAA 
ATGCAAGCGA GGCTCCTGTC TTCCTCGGTA GACACCCCTC AGCAGCTGCA GTCGGTCCTG 
GAGTCGCTCA TCGCCAAGAA GCAGAGCCTC TGTGAAGTGC TGCAGGCCTG GAATAACAGG 
TTGCAGGACC TTTTCCAGCA GGAAAAGGGT AGAAAGCGGC CTTCAGTTCC TCCAAGTCCT 
GGAAGACTGA GACAAGGGGA AGAAAGCAAG ATAAGTGCAA TGGATGCATC TCCACGGAAT 
ATTTCTCCAG GACTTCAAAA TGGAGAAAAA GAGGACCGCT TCTTGACAAC CCTCTCTAGT 
CAGGGCCCCA CCTCTGCTGC TCATCTCCAG CTGCCCAGTC CCCCTGAAGT CCTGCCTGAG 
CAGGCAGGGG GGGCGACCCC CGAGCCGGAT GCAGCCAGCA GTTCTGAAGA TGTGTTTGAT 
GGACATTCTC TGGGATCCAC AGACAGCCAG GTGAAGGAAA AATCAACCAT GAAGGCCATC 
TTTGCAAATT TGCTTCCAGG AAATAGCTAT AACCCTATTC CGTTTCCTTT TGATCCAGAT 
AAACACTACT TAATGTACGA ACACGAACGG GTGCCCATTG CCGTCTGTGA GAGGGAGCCC 
AGCTCCATCA TTGCTTTTGC TCTCAGTTGT AAAGAATACC GAAATGCCTT AGAGGAATTG 
TCCAAGGCAA CTCTGCGGAA CAGTGCCGAA GAAGGGCTCC CAGCCAACAG TGCTTCAGAC 
AGCAGACCAA AGGGCGGCAG CCCTGGGAGG CCGCCTGAGC TCGGTGGAGG CTGCCCCAAC 
CGGGCGGCCG AGGCAGAGCC GCAGCCAGCC AAAAAGGCTT CTGGGATGTT GTCCTTCTTC 
CGAGGGACAG CAGGGAAAAG CCCTGATCTC TCTTCCCAGA AGAGAGAGAC CTTACGTGGA 
GCAGATAGTG CTTACTACCA GGTCGGGCAG CCCGGCAAGG AGGGGACGGA GAGTCAAGGC 
ACTGAGCCTC CAGATGAGAT AGATGGAGGA GACGCACAAA AGAAACAGCT CACAAATCCT 
CATGTGGAAC TTCAATTTTC TGACGCTAAC GCCAAGTTTT ACTGCCGGCT CTACTATGCG 
GGAGAGTTTC ACAAGATGCG CGAGGTGATC CTGGGCAGCA GCGAGGAGGA CTTCATCCGC 
TCCCTTTCCC ACTCGGCACC CTGGCAGGCA CGCGGGGGCA AGTCCGGAGC GGCCTTCTAC 
GCCACGGAAG ATGATAGATT CATTTTGAAG CAGATGCCTC GTCTGGAAGT CCAGTCTTTC 
CTCGACTTTG CACCACACTA CTTCAATTAT ATCACAAACG CTGTGCAACA AAAGAGGCCT 
ACTGCACTGG CCAAAATTCT TGGAGTTTAC AGAATTGGTT ATAAGAATTC TCAGAACAAC 
ACTGAGAAGA AGTTAGATCT CCTTGTCATG GAAAATCTTT TCTATGGGAG AAAGATGGCA 
CAGGTTTTTG ACTTGAAGGG TTCACTTAGG AACAGAAATG TAAAAACTGA CACCGGGAAA 
GAGAGCTGTG ACGTGGTCCT GCTGGACGAG AATCTGCTGA AGATGGTCCG GGACAGCCCT 
CTGTACATCC GCTCGCACTC CAAGGCGGTG CTGCGGGCCT CCATCCGCAG CGATGCCCAC 
TTCCTGTCCA GCCACCTCAT CATCGACTAT TCTTTGCTGG TGGGGCGAGA TGATACCAGC 
AATGAGCTTG TCGTTGGGAT TATAGATTAT ATTCGAACGT TTACATGGGA CAAAAAGCTT 
GAGATGGTTG TGAAATCAAC AGGAATTTTA GGAGGACAAG GTAAAATGCC AACTGTGGTC 
TCTCCAGAGT TGTACAGGAC CCGATTTTGT GAAGCGATGG ACAAGTATTT CCTCATGGTA 
CCAGACCACT GGACAGGCTT GGGTCTCAAC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_023622518.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023478286.1 Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X2, mRNA.

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CloneID	OHb52469
Clone ID Related Accession (Same CDS sequence)	XM_023622519.1 , XM_023622517.1 , XM_023622518.1 , XM_023622520.1
Accession Version	XM_023622518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGCAGA GAACAGTTTG CAGCACCCCC AGGAGAACAC AGACACAAGA 
AGGAAAACAG AACCTACCTT TGGCGGTCAT GACCCTCGTA CAGCTGTTCA GCTTCGAAGC 
CTCAGCACAG TGTTAAAACG CCTCAAGGAA ATCATGGAGG GGAAAAGCCA GGACAGTGAC 
CTGAAGCAGT ACTGGATGCC GGACAGCCAG TGCAGAGAGT GCTACGACTG CAGCGAGAAG 
TTCACCACCT TCCGGCGCAG GCACCACTGC CGGCTCTGCG GGCAGATTTT CTGCAGCCGC 
TGCTGCAATC AAGAGATCCC TGGGAAGTTT ATGGGCTATA CAGGGGACCT CCGAGCTTGC 
ACATACTGTA GAAAAATAGC CTTAAGTTAT GCTCATTCCA CAGACAGTAA TTCCATTGGG 
GAAGACTTGA ATGCTCTTTC AGATTCTGCT TCCTCTGTGT CTGTACTTGA TCCGAGTGAA 
CCCCGAACAC CTGTTGGGAG TAGGAAAGCC AGCCGGAACA TCTTTTTAGA GGATGATTTT 
GCCTGGCAAA GTTTGATTCA TCCAGACTCC TCAAATACTG CTCTTTCAAC AAGACTTGTA 
TCTGTTCAAG AGGACGCTGG GAAATCTCCT GCTCGAAATA GAATGGACTT GGCAGTGCCT 
GCTCCATTGG ATGCTGTGTG GATTAAATCA TACATACATG GCCCTACTGG GTCAGCCAGC 
ATTACCAACC TCTCACTGGA TCGATCTGGT TCTCCCCTGG TACCTTCATA TGAGACATCT 
GTCAGTCCCC AGGCCAACCG AACATACGTG AGGACAGAGA CCACAGAGGA TGAACGCAAA 
ATTCTTCTGG ACAGCGTCCA GTTAAAAGAC CTGTGGAAGA AAATCTGCCA TCACAGTAGT 
GGAATGGAGT TTCAGGATCA CCGCTACTGG CTGAGGACAC ATCCCAACTG CATCGTAGGG 
AAGGAGTTAG TCAACTGGCT AATCCGAAAT GGGCACATTG CCACAAGGGC ACAAGCTGTA 
GCAATTGGAC AAGCGATGGT GGACGGACGG TGGCTGGACT GCGTGAGCCA CCATGACCAG 
CTCTTCAGGG ATGAGTATGC GCTCTACAGA CCCCTGCAGA GTACAGACTT TTCTGAGACC 
CCTTCTCCGG ACAGTGACTC CGTGAACTCT GTGGAAGGAC ACTCGGAGCC ATCATGGTTT 
AAAGACATAA AGTTTGATGA CAGCGACACA GAGCAGATTG CTGAAGAAGG TGACGATAAT 
TTGACTAACT CTGCCAGTCC TAGCAAGCGC ACGTCAGTCA GCAGTTTCCA GTCCACAGTG 
GACAGTGACT CAGCCGCCTC CATCAGCCTG AACGTGGAGT TGGACAACGT GAACTTCCAC 
ATCAAGAAGC CCTCCAAGTA CCCACACGTG CCCCCTCACC CTGCTGACCA AAAAGAGTAT 
TTGATTTCTG ACAACGGAGG ACAACAACTC TCAATAAGTG ACGCCTTCAT CAAAGAATCC 
TTATTTAATC GTCGAGTTGA GGAGAAATCC AAAGAACTGC CCTTTACGCC TTTGGGCTGG 
CATCATAACA ATCTGGAGCT GCTGCGGGAG GAGAATGGAG AGAAGCAGGC CATGGAGAGG 
TTGCTTTCAG CTAATCATAA CCACATGATG GCACTGCTCC AACAGTTGCT CCATAGTGAG 
TCATTGTCAC CATCTTGGAG GGACATCATT GTATCCCTGG TCTGCCAGGT TGTTCAGACA 
GTCCGACCTG ACGTCAAGAA CCGGGATGAC GACATGGATA TCCGTCAGTT TGTCCACGTC 
AAAAAAATCC CAGGTGGAAA GAAGTTTGAT TCTATGGTTG TCAATGGGTT CGTTTGTACC 
AAGAATATTG CACATAAAAA GATGAATTCT TGTATTAAAA ACCCCAAAAT TCTTCTGTTG 
AAGTGTTCCA TTGAGTATCT CTACAGAGAA GAAACTAAAT TTACTTGCAT TGACCCTATC 
GTGCTTCAGG AAAGGGAATT CTTGAAGAAT TATGTTCAGC GAATAGTGGA TGTTCGACCC 
ACATTGGTTC TAGTTGAGAA AACGGTGTCT CGGATTGCCC AGGACCTGTT GCTGGAACAC 
GGCATCACTT TGGTCATTAA TGTCAAATCA CAAGTTTTAG AACGAATCAG TCGGATGACC 
CAAGGTGATT TAGTGATGTC GATGGACCAG TTGCTCACAA AGCCCCACCT GGGCACCTGT 
CACAAATTTT ACATGCAGAT GTTTCAGCTA CCTGGTGAAC AAACCAAAAC TCTGATGTTT 
TTTGAAGGCT GTCCACAGCA CCTGGGCTGT ACAATCAAGC TTAGAGGAGG CTCCGATTAT 
GAGCTGGCTC GAGTTAAGGA GATCCTCATA TTTATGATCT GTGTAGCATA TCATTCTCAA 
CTAGAAATCT CCTTCCTCAT GGATGAATTT GCCATGCCTC CTACATTAAC GCAAAACCCT 
TCCTTCCACT CTCTGATTGA GGAACAGGAG GATGACGAGG CTGCTCAAGA ACAGCTCAGC 
GGAAGTCCCC TCCCCCGGGA ACCTGACTTC CCTCTGGAGT GTCTGGCCCC TGATGAGAGC 
AGTTCACTGG AATCAAGGAC TGTGTTTGGG AAGAGTGACC AGGAACTTAA AGGTGCTCCA 
AAGGATGCTG TCTCGTTGAA GCAGCAGGAG TTCGCCACAG CAGCCTGCCC GGCCGGCGTC 
CCCTATGCTC TCTTTGCATT GGTCCCAGAA TCCTTGTTGC CACTGCATCT GGGTGACCAA 
CAGGATGCCA GGGGCAGCGA GCAGCCAGAG ACTGTGCAGC AAACGGGTGA GCTACAGGAT 
CCCAAAAGCC AGATGAGAGC CTTTAGAGAC CCTCTGCAGG ATGACACTGG ATTGTATGTC 
ACTGAGGAAG TCACCTCTTC TGAGGATAAG CGGAAGACTT ACTCTTTGGC CTTTAAACAG 
GAATTAAAAG ACGTAATTCT CTGTATCTCC CCAGTGATCA CATTCCGAGA GCCTTTCCTT 
TTAACCGAGA AGGGGATGCG ATGCTCTCCC CGAGACTATT TCGCAGAGCA GCTTTACTGG 
TCTCCTCTCC TCAATAAAGA CTTCAAAGAA ATGGAGAGCA GGCGGAAGAA GCAGCTGCTC 
AGGGATCTCT CCAGCCTCCA GGGCCTGAAC GGAAGTGTGC AGGCCAAGTC TATCCAAGTG 
CTGCCCTCAC ACGAGCTGGT GAGCGCTAGG ATTGCTGAGC ATCTGGGTGA CAGCCAGGGC 
CTGGGCCGGA TGCTGGCTGA CTACCGGGCC AGAGGAGGGA GAATTCAGCA GAATACTTCA 
GACCCTTTTG CTCATTCAAA GGATGCATCA GGTCCTTCAA GTGGCAAGTC AGGAAGCAGA 
ACTGAGGGCG ATGAAGAGAA AGGGCTGATT CCGAGTGACG CGGTATGGTC AACAAAGGTG 
GACTGTCTGA ACCCTGCCAA CCACCAGAGG CTGTGCGTGC TCTTCAGCAG CTCTTCTGCC 
CAGTCCAGCA ACGCGCCCAG CGCCTGCGTC AGTCCTTGGA TTGTAACAAT GGAATTTTAT 
GGAAAGAATG ATCTTACACT GGGAATATTT TTAGAGAGAT ACTGTTTCAG GCCTTCTTAC 
CAGTGTCCTA GCATGTTCTG TGATACCCCC ATGGTACATC ACATTCGGCG CTTTGTCCAT 
GGCCAAGGCT GTGTGCAGAT AATCCTGAAG GAGCTGGATT CTCCAGTACC TGGATATCAG 
CATACAATTC TCACCTATTC CTGGTGCAGA ATCTGCAAAC AGGTAACACC AGTTGTTGCT 
CTTTCCAATG AGTCCTGGTC CATGTCATTT GCCAAATACC TTGAACTTCG GTTTTATGGG 
CACCAGTACA CCCGTAGAGC CAATGCTGAG CCGTGTGGCC ACTCCATCCA CCATGATTAT 
CACCAGTACT TCTCCTATAA CCAGATGGTG GCATCTTTTA GTTATTCTCC CATTCGGCTT 
CTTGAAGTAT GTGTTCCACT CCCCAAAATA TTCATTAAAC GTCAGGCCCC ATTAAAAGTG 
TCCCTTCTTC AAGATCTGAA GGACTTCTTT CAAAAAGTTT CACAAGTATA TCTTGCTGTT 
GATGAAAGAC TTGCATCTTT GAAAACTGAT ACATTTAGCA AAACAAGAGA GGAAAAAATG 
GAAGATATCT TTGCACAAAA AGAGATGGAG GAAGGCGAGT TCAAGAACTG GATTGAGAAA 
ATGCAAGCGA GGCTCCTGTC TTCCTCGGTA GACACCCCTC AGCAGCTGCA GTCGGTCCTG 
GAGTCGCTCA TCGCCAAGAA GCAGAGCCTC TGTGAAGTGC TGCAGGCCTG GAATAACAGG 
TTGCAGGACC TTTTCCAGCA GGAAAAGGGT AGAAAGCGGC CTTCAGTTCC TCCAAGTCCT 
GGAAGACTGA GACAAGGGGA AGAAAGCAAG ATAAGTGCAA TGGATGCATC TCCACGGAAT 
ATTTCTCCAG GACTTCAAAA TGGAGAAAAA GAGGACCGCT TCTTGACAAC CCTCTCTAGT 
CAGGGCCCCA CCTCTGCTGC TCATCTCCAG CTGCCCAGTC CCCCTGAAGT CCTGCCTGAG 
CAGGCAGGGG GGGCGACCCC CGAGCCGGAT GCAGCCAGCA GTTCTGAAGA TGTGTTTGAT 
GGACATTCTC TGGGATCCAC AGACAGCCAG GTGAAGGAAA AATCAACCAT GAAGGCCATC 
TTTGCAAATT TGCTTCCAGG AAATAGCTAT AACCCTATTC CGTTTCCTTT TGATCCAGAT 
AAACACTACT TAATGTACGA ACACGAACGG GTGCCCATTG CCGTCTGTGA GAGGGAGCCC 
AGCTCCATCA TTGCTTTTGC TCTCAGTTGT AAAGAATACC GAAATGCCTT AGAGGAATTG 
TCCAAGGCAA CTCTGCGGAA CAGTGCCGAA GAAGGGCTCC CAGCCAACAG TGCTTCAGAC 
AGCAGACCAA AGGGCGGCAG CCCTGGGAGG CCGCCTGAGC TCGGTGGAGG CTGCCCCAAC 
CGGGCGGCCG AGGCAGAGCC GCAGCCAGCC AAAAAGGCTT CTGGGATGTT GTCCTTCTTC 
CGAGGGACAG CAGGGAAAAG CCCTGATCTC TCTTCCCAGA AGAGAGAGAC CTTACGTGGA 
GCAGATAGTG CTTACTACCA GGTCGGGCAG CCCGGCAAGG AGGGGACGGA GAGTCAAGGC 
ACTGAGCCTC CAGATGAGAT AGATGGAGGA GACGCACAAA AGAAACAGCT CACAAATCCT 
CATGTGGAAC TTCAATTTTC TGACGCTAAC GCCAAGTTTT ACTGCCGGCT CTACTATGCG 
GGAGAGTTTC ACAAGATGCG CGAGGTGATC CTGGGCAGCA GCGAGGAGGA CTTCATCCGC 
TCCCTTTCCC ACTCGGCACC CTGGCAGGCA CGCGGGGGCA AGTCCGGAGC GGCCTTCTAC 
GCCACGGAAG ATGATAGATT CATTTTGAAG CAGATGCCTC GTCTGGAAGT CCAGTCTTTC 
CTCGACTTTG CACCACACTA CTTCAATTAT ATCACAAACG CTGTGCAACA AAAGAGGCCT 
ACTGCACTGG CCAAAATTCT TGGAGTTTAC AGAATTGGTT ATAAGAATTC TCAGAACAAC 
ACTGAGAAGA AGTTAGATCT CCTTGTCATG GAAAATCTTT TCTATGGGAG AAAGATGGCA 
CAGGTTTTTG ACTTGAAGGG TTCACTTAGG AACAGAAATG TAAAAACTGA CACCGGGAAA 
GAGAGCTGTG ACGTGGTCCT GCTGGACGAG AATCTGCTGA AGATGGTCCG GGACAGCCCT 
CTGTACATCC GCTCGCACTC CAAGGCGGTG CTGCGGGCCT CCATCCGCAG CGATGCCCAC 
TTCCTGTCCA GCCACCTCAT CATCGACTAT TCTTTGCTGG TGGGGCGAGA TGATACCAGC 
AATGAGCTTG TCGTTGGGAT TATAGATTAT ATTCGAACGT TTACATGGGA CAAAAAGCTT 
GAGATGGTTG TGAAATCAAC AGGAATTTTA GGAGGACAAG GTAAAATGCC AACTGTGGTC 
TCTCCAGAGT TGTACAGGAC CCGATTTTGT GAAGCGATGG ACAAGTATTT CCTCATGGTA 
CCAGACCACT GGACAGGCTT GGGTCTCAAC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023478286.1
CDS	99..6494
Translation	MATDDKTSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPAFKSAYSSFVNLFRFNKDRAEGEQQSLTGGWPSPQLPSRTQSVRSPIPYKKQLTEELQRRPSAVLAENSLQHPQENTDTRRKTEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCRECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRMDLAVPAPLDAVWIKSYIHGPTGSASITNLSLDRSGSPLVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAVAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTDFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSPSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHVKKIPGGKKFDSMVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDLLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPGEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEEQEDDEAAQEQLSGSPLPREPDFPLECLAPDESSSLESRTVFGKSDQELKGAPKDAVSLKQQEFATAACPAGVPYALFALVPESLLPLHLGDQQDARGSEQPETVQQTGELQDPKSQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSPRDYFAEQLYWSPLLNKDFKEMESRRKKQLLRDLSSLQGLNGSVQAKSIQVLPSHELVSARIAEHLGDSQGLGRMLADYRARGGRIQQNTSDPFAHSKDASGPSSGKSGSRTEGDEEKGLIPSDAVWSTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLLSSSVDTPQQLQSVLESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQGPTSAAHLQLPSPPEVLPEQAGGATPEPDAASSSEDVFDGHSLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSASDSRPKGGSPGRPPELGGGCPNRAAEAEPQPAKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQPGKEGTESQGTEPPDEIDGGDAQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSAPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDSPLYIRSHSKAVLRASIRSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_023622518.1
Organism	Equus caballus(horse)
Definition	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023622518.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGCAGA GAACAGTTTG CAGCACCCCC AGGAGAACAC AGACACAAGA 
AGGAAAACAG AACCTACCTT TGGCGGTCAT GACCCTCGTA CAGCTGTTCA GCTTCGAAGC 
CTCAGCACAG TGTTAAAACG CCTCAAGGAA ATCATGGAGG GGAAAAGCCA GGACAGTGAC 
CTGAAGCAGT ACTGGATGCC GGACAGCCAG TGCAGAGAGT GCTACGACTG CAGCGAGAAG 
TTCACCACCT TCCGGCGCAG GCACCACTGC CGGCTCTGCG GGCAGATTTT CTGCAGCCGC 
TGCTGCAATC AAGAGATCCC TGGGAAGTTT ATGGGCTATA CAGGGGACCT CCGAGCTTGC 
ACATACTGTA GAAAAATAGC CTTAAGTTAT GCTCATTCCA CAGACAGTAA TTCCATTGGG 
GAAGACTTGA ATGCTCTTTC AGATTCTGCT TCCTCTGTGT CTGTACTTGA TCCGAGTGAA 
CCCCGAACAC CTGTTGGGAG TAGGAAAGCC AGCCGGAACA TCTTTTTAGA GGATGATTTT 
GCCTGGCAAA GTTTGATTCA TCCAGACTCC TCAAATACTG CTCTTTCAAC AAGACTTGTA 
TCTGTTCAAG AGGACGCTGG GAAATCTCCT GCTCGAAATA GAATGGACTT GGCAGTGCCT 
GCTCCATTGG ATGCTGTGTG GATTAAATCA TACATACATG GCCCTACTGG GTCAGCCAGC 
ATTACCAACC TCTCACTGGA TCGATCTGGT TCTCCCCTGG TACCTTCATA TGAGACATCT 
GTCAGTCCCC AGGCCAACCG AACATACGTG AGGACAGAGA CCACAGAGGA TGAACGCAAA 
ATTCTTCTGG ACAGCGTCCA GTTAAAAGAC CTGTGGAAGA AAATCTGCCA TCACAGTAGT 
GGAATGGAGT TTCAGGATCA CCGCTACTGG CTGAGGACAC ATCCCAACTG CATCGTAGGG 
AAGGAGTTAG TCAACTGGCT AATCCGAAAT GGGCACATTG CCACAAGGGC ACAAGCTGTA 
GCAATTGGAC AAGCGATGGT GGACGGACGG TGGCTGGACT GCGTGAGCCA CCATGACCAG 
CTCTTCAGGG ATGAGTATGC GCTCTACAGA CCCCTGCAGA GTACAGACTT TTCTGAGACC 
CCTTCTCCGG ACAGTGACTC CGTGAACTCT GTGGAAGGAC ACTCGGAGCC ATCATGGTTT 
AAAGACATAA AGTTTGATGA CAGCGACACA GAGCAGATTG CTGAAGAAGG TGACGATAAT 
TTGACTAACT CTGCCAGTCC TAGCAAGCGC ACGTCAGTCA GCAGTTTCCA GTCCACAGTG 
GACAGTGACT CAGCCGCCTC CATCAGCCTG AACGTGGAGT TGGACAACGT GAACTTCCAC 
ATCAAGAAGC CCTCCAAGTA CCCACACGTG CCCCCTCACC CTGCTGACCA AAAAGAGTAT 
TTGATTTCTG ACAACGGAGG ACAACAACTC TCAATAAGTG ACGCCTTCAT CAAAGAATCC 
TTATTTAATC GTCGAGTTGA GGAGAAATCC AAAGAACTGC CCTTTACGCC TTTGGGCTGG 
CATCATAACA ATCTGGAGCT GCTGCGGGAG GAGAATGGAG AGAAGCAGGC CATGGAGAGG 
TTGCTTTCAG CTAATCATAA CCACATGATG GCACTGCTCC AACAGTTGCT CCATAGTGAG 
TCATTGTCAC CATCTTGGAG GGACATCATT GTATCCCTGG TCTGCCAGGT TGTTCAGACA 
GTCCGACCTG ACGTCAAGAA CCGGGATGAC GACATGGATA TCCGTCAGTT TGTCCACGTC 
AAAAAAATCC CAGGTGGAAA GAAGTTTGAT TCTATGGTTG TCAATGGGTT CGTTTGTACC 
AAGAATATTG CACATAAAAA GATGAATTCT TGTATTAAAA ACCCCAAAAT TCTTCTGTTG 
AAGTGTTCCA TTGAGTATCT CTACAGAGAA GAAACTAAAT TTACTTGCAT TGACCCTATC 
GTGCTTCAGG AAAGGGAATT CTTGAAGAAT TATGTTCAGC GAATAGTGGA TGTTCGACCC 
ACATTGGTTC TAGTTGAGAA AACGGTGTCT CGGATTGCCC AGGACCTGTT GCTGGAACAC 
GGCATCACTT TGGTCATTAA TGTCAAATCA CAAGTTTTAG AACGAATCAG TCGGATGACC 
CAAGGTGATT TAGTGATGTC GATGGACCAG TTGCTCACAA AGCCCCACCT GGGCACCTGT 
CACAAATTTT ACATGCAGAT GTTTCAGCTA CCTGGTGAAC AAACCAAAAC TCTGATGTTT 
TTTGAAGGCT GTCCACAGCA CCTGGGCTGT ACAATCAAGC TTAGAGGAGG CTCCGATTAT 
GAGCTGGCTC GAGTTAAGGA GATCCTCATA TTTATGATCT GTGTAGCATA TCATTCTCAA 
CTAGAAATCT CCTTCCTCAT GGATGAATTT GCCATGCCTC CTACATTAAC GCAAAACCCT 
TCCTTCCACT CTCTGATTGA GGAACAGGAG GATGACGAGG CTGCTCAAGA ACAGCTCAGC 
GGAAGTCCCC TCCCCCGGGA ACCTGACTTC CCTCTGGAGT GTCTGGCCCC TGATGAGAGC 
AGTTCACTGG AATCAAGGAC TGTGTTTGGG AAGAGTGACC AGGAACTTAA AGGTGCTCCA 
AAGGATGCTG TCTCGTTGAA GCAGCAGGAG TTCGCCACAG CAGCCTGCCC GGCCGGCGTC 
CCCTATGCTC TCTTTGCATT GGTCCCAGAA TCCTTGTTGC CACTGCATCT GGGTGACCAA 
CAGGATGCCA GGGGCAGCGA GCAGCCAGAG ACTGTGCAGC AAACGGGTGA GCTACAGGAT 
CCCAAAAGCC AGATGAGAGC CTTTAGAGAC CCTCTGCAGG ATGACACTGG ATTGTATGTC 
ACTGAGGAAG TCACCTCTTC TGAGGATAAG CGGAAGACTT ACTCTTTGGC CTTTAAACAG 
GAATTAAAAG ACGTAATTCT CTGTATCTCC CCAGTGATCA CATTCCGAGA GCCTTTCCTT 
TTAACCGAGA AGGGGATGCG ATGCTCTCCC CGAGACTATT TCGCAGAGCA GCTTTACTGG 
TCTCCTCTCC TCAATAAAGA CTTCAAAGAA ATGGAGAGCA GGCGGAAGAA GCAGCTGCTC 
AGGGATCTCT CCAGCCTCCA GGGCCTGAAC GGAAGTGTGC AGGCCAAGTC TATCCAAGTG 
CTGCCCTCAC ACGAGCTGGT GAGCGCTAGG ATTGCTGAGC ATCTGGGTGA CAGCCAGGGC 
CTGGGCCGGA TGCTGGCTGA CTACCGGGCC AGAGGAGGGA GAATTCAGCA GAATACTTCA 
GACCCTTTTG CTCATTCAAA GGATGCATCA GGTCCTTCAA GTGGCAAGTC AGGAAGCAGA 
ACTGAGGGCG ATGAAGAGAA AGGGCTGATT CCGAGTGACG CGGTATGGTC AACAAAGGTG 
GACTGTCTGA ACCCTGCCAA CCACCAGAGG CTGTGCGTGC TCTTCAGCAG CTCTTCTGCC 
CAGTCCAGCA ACGCGCCCAG CGCCTGCGTC AGTCCTTGGA TTGTAACAAT GGAATTTTAT 
GGAAAGAATG ATCTTACACT GGGAATATTT TTAGAGAGAT ACTGTTTCAG GCCTTCTTAC 
CAGTGTCCTA GCATGTTCTG TGATACCCCC ATGGTACATC ACATTCGGCG CTTTGTCCAT 
GGCCAAGGCT GTGTGCAGAT AATCCTGAAG GAGCTGGATT CTCCAGTACC TGGATATCAG 
CATACAATTC TCACCTATTC CTGGTGCAGA ATCTGCAAAC AGGTAACACC AGTTGTTGCT 
CTTTCCAATG AGTCCTGGTC CATGTCATTT GCCAAATACC TTGAACTTCG GTTTTATGGG 
CACCAGTACA CCCGTAGAGC CAATGCTGAG CCGTGTGGCC ACTCCATCCA CCATGATTAT 
CACCAGTACT TCTCCTATAA CCAGATGGTG GCATCTTTTA GTTATTCTCC CATTCGGCTT 
CTTGAAGTAT GTGTTCCACT CCCCAAAATA TTCATTAAAC GTCAGGCCCC ATTAAAAGTG 
TCCCTTCTTC AAGATCTGAA GGACTTCTTT CAAAAAGTTT CACAAGTATA TCTTGCTGTT 
GATGAAAGAC TTGCATCTTT GAAAACTGAT ACATTTAGCA AAACAAGAGA GGAAAAAATG 
GAAGATATCT TTGCACAAAA AGAGATGGAG GAAGGCGAGT TCAAGAACTG GATTGAGAAA 
ATGCAAGCGA GGCTCCTGTC TTCCTCGGTA GACACCCCTC AGCAGCTGCA GTCGGTCCTG 
GAGTCGCTCA TCGCCAAGAA GCAGAGCCTC TGTGAAGTGC TGCAGGCCTG GAATAACAGG 
TTGCAGGACC TTTTCCAGCA GGAAAAGGGT AGAAAGCGGC CTTCAGTTCC TCCAAGTCCT 
GGAAGACTGA GACAAGGGGA AGAAAGCAAG ATAAGTGCAA TGGATGCATC TCCACGGAAT 
ATTTCTCCAG GACTTCAAAA TGGAGAAAAA GAGGACCGCT TCTTGACAAC CCTCTCTAGT 
CAGGGCCCCA CCTCTGCTGC TCATCTCCAG CTGCCCAGTC CCCCTGAAGT CCTGCCTGAG 
CAGGCAGGGG GGGCGACCCC CGAGCCGGAT GCAGCCAGCA GTTCTGAAGA TGTGTTTGAT 
GGACATTCTC TGGGATCCAC AGACAGCCAG GTGAAGGAAA AATCAACCAT GAAGGCCATC 
TTTGCAAATT TGCTTCCAGG AAATAGCTAT AACCCTATTC CGTTTCCTTT TGATCCAGAT 
AAACACTACT TAATGTACGA ACACGAACGG GTGCCCATTG CCGTCTGTGA GAGGGAGCCC 
AGCTCCATCA TTGCTTTTGC TCTCAGTTGT AAAGAATACC GAAATGCCTT AGAGGAATTG 
TCCAAGGCAA CTCTGCGGAA CAGTGCCGAA GAAGGGCTCC CAGCCAACAG TGCTTCAGAC 
AGCAGACCAA AGGGCGGCAG CCCTGGGAGG CCGCCTGAGC TCGGTGGAGG CTGCCCCAAC 
CGGGCGGCCG AGGCAGAGCC GCAGCCAGCC AAAAAGGCTT CTGGGATGTT GTCCTTCTTC 
CGAGGGACAG CAGGGAAAAG CCCTGATCTC TCTTCCCAGA AGAGAGAGAC CTTACGTGGA 
GCAGATAGTG CTTACTACCA GGTCGGGCAG CCCGGCAAGG AGGGGACGGA GAGTCAAGGC 
ACTGAGCCTC CAGATGAGAT AGATGGAGGA GACGCACAAA AGAAACAGCT CACAAATCCT 
CATGTGGAAC TTCAATTTTC TGACGCTAAC GCCAAGTTTT ACTGCCGGCT CTACTATGCG 
GGAGAGTTTC ACAAGATGCG CGAGGTGATC CTGGGCAGCA GCGAGGAGGA CTTCATCCGC 
TCCCTTTCCC ACTCGGCACC CTGGCAGGCA CGCGGGGGCA AGTCCGGAGC GGCCTTCTAC 
GCCACGGAAG ATGATAGATT CATTTTGAAG CAGATGCCTC GTCTGGAAGT CCAGTCTTTC 
CTCGACTTTG CACCACACTA CTTCAATTAT ATCACAAACG CTGTGCAACA AAAGAGGCCT 
ACTGCACTGG CCAAAATTCT TGGAGTTTAC AGAATTGGTT ATAAGAATTC TCAGAACAAC 
ACTGAGAAGA AGTTAGATCT CCTTGTCATG GAAAATCTTT TCTATGGGAG AAAGATGGCA 
CAGGTTTTTG ACTTGAAGGG TTCACTTAGG AACAGAAATG TAAAAACTGA CACCGGGAAA 
GAGAGCTGTG ACGTGGTCCT GCTGGACGAG AATCTGCTGA AGATGGTCCG GGACAGCCCT 
CTGTACATCC GCTCGCACTC CAAGGCGGTG CTGCGGGCCT CCATCCGCAG CGATGCCCAC 
TTCCTGTCCA GCCACCTCAT CATCGACTAT TCTTTGCTGG TGGGGCGAGA TGATACCAGC 
AATGAGCTTG TCGTTGGGAT TATAGATTAT ATTCGAACGT TTACATGGGA CAAAAAGCTT 
GAGATGGTTG TGAAATCAAC AGGAATTTTA GGAGGACAAG GTAAAATGCC AACTGTGGTC 
TCTCCAGAGT TGTACAGGAC CCGATTTTGT GAAGCGATGG ACAAGTATTT CCTCATGGTA 
CCAGACCACT GGACAGGCTT GGGTCTCAAC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_023622520.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023478288.1 Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X4, mRNA.

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CloneID	OHb52469
Clone ID Related Accession (Same CDS sequence)	XM_023622519.1 , XM_023622517.1 , XM_023622518.1 , XM_023622520.1
Accession Version	XM_023622520.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGCAGA GAACAGTTTG CAGCACCCCC AGGAGAACAC AGACACAAGA 
AGGAAAACAG AACCTACCTT TGGCGGTCAT GACCCTCGTA CAGCTGTTCA GCTTCGAAGC 
CTCAGCACAG TGTTAAAACG CCTCAAGGAA ATCATGGAGG GGAAAAGCCA GGACAGTGAC 
CTGAAGCAGT ACTGGATGCC GGACAGCCAG TGCAGAGAGT GCTACGACTG CAGCGAGAAG 
TTCACCACCT TCCGGCGCAG GCACCACTGC CGGCTCTGCG GGCAGATTTT CTGCAGCCGC 
TGCTGCAATC AAGAGATCCC TGGGAAGTTT ATGGGCTATA CAGGGGACCT CCGAGCTTGC 
ACATACTGTA GAAAAATAGC CTTAAGTTAT GCTCATTCCA CAGACAGTAA TTCCATTGGG 
GAAGACTTGA ATGCTCTTTC AGATTCTGCT TCCTCTGTGT CTGTACTTGA TCCGAGTGAA 
CCCCGAACAC CTGTTGGGAG TAGGAAAGCC AGCCGGAACA TCTTTTTAGA GGATGATTTT 
GCCTGGCAAA GTTTGATTCA TCCAGACTCC TCAAATACTG CTCTTTCAAC AAGACTTGTA 
TCTGTTCAAG AGGACGCTGG GAAATCTCCT GCTCGAAATA GAATGGACTT GGCAGTGCCT 
GCTCCATTGG ATGCTGTGTG GATTAAATCA TACATACATG GCCCTACTGG GTCAGCCAGC 
ATTACCAACC TCTCACTGGA TCGATCTGGT TCTCCCCTGG TACCTTCATA TGAGACATCT 
GTCAGTCCCC AGGCCAACCG AACATACGTG AGGACAGAGA CCACAGAGGA TGAACGCAAA 
ATTCTTCTGG ACAGCGTCCA GTTAAAAGAC CTGTGGAAGA AAATCTGCCA TCACAGTAGT 
GGAATGGAGT TTCAGGATCA CCGCTACTGG CTGAGGACAC ATCCCAACTG CATCGTAGGG 
AAGGAGTTAG TCAACTGGCT AATCCGAAAT GGGCACATTG CCACAAGGGC ACAAGCTGTA 
GCAATTGGAC AAGCGATGGT GGACGGACGG TGGCTGGACT GCGTGAGCCA CCATGACCAG 
CTCTTCAGGG ATGAGTATGC GCTCTACAGA CCCCTGCAGA GTACAGACTT TTCTGAGACC 
CCTTCTCCGG ACAGTGACTC CGTGAACTCT GTGGAAGGAC ACTCGGAGCC ATCATGGTTT 
AAAGACATAA AGTTTGATGA CAGCGACACA GAGCAGATTG CTGAAGAAGG TGACGATAAT 
TTGACTAACT CTGCCAGTCC TAGCAAGCGC ACGTCAGTCA GCAGTTTCCA GTCCACAGTG 
GACAGTGACT CAGCCGCCTC CATCAGCCTG AACGTGGAGT TGGACAACGT GAACTTCCAC 
ATCAAGAAGC CCTCCAAGTA CCCACACGTG CCCCCTCACC CTGCTGACCA AAAAGAGTAT 
TTGATTTCTG ACAACGGAGG ACAACAACTC TCAATAAGTG ACGCCTTCAT CAAAGAATCC 
TTATTTAATC GTCGAGTTGA GGAGAAATCC AAAGAACTGC CCTTTACGCC TTTGGGCTGG 
CATCATAACA ATCTGGAGCT GCTGCGGGAG GAGAATGGAG AGAAGCAGGC CATGGAGAGG 
TTGCTTTCAG CTAATCATAA CCACATGATG GCACTGCTCC AACAGTTGCT CCATAGTGAG 
TCATTGTCAC CATCTTGGAG GGACATCATT GTATCCCTGG TCTGCCAGGT TGTTCAGACA 
GTCCGACCTG ACGTCAAGAA CCGGGATGAC GACATGGATA TCCGTCAGTT TGTCCACGTC 
AAAAAAATCC CAGGTGGAAA GAAGTTTGAT TCTATGGTTG TCAATGGGTT CGTTTGTACC 
AAGAATATTG CACATAAAAA GATGAATTCT TGTATTAAAA ACCCCAAAAT TCTTCTGTTG 
AAGTGTTCCA TTGAGTATCT CTACAGAGAA GAAACTAAAT TTACTTGCAT TGACCCTATC 
GTGCTTCAGG AAAGGGAATT CTTGAAGAAT TATGTTCAGC GAATAGTGGA TGTTCGACCC 
ACATTGGTTC TAGTTGAGAA AACGGTGTCT CGGATTGCCC AGGACCTGTT GCTGGAACAC 
GGCATCACTT TGGTCATTAA TGTCAAATCA CAAGTTTTAG AACGAATCAG TCGGATGACC 
CAAGGTGATT TAGTGATGTC GATGGACCAG TTGCTCACAA AGCCCCACCT GGGCACCTGT 
CACAAATTTT ACATGCAGAT GTTTCAGCTA CCTGGTGAAC AAACCAAAAC TCTGATGTTT 
TTTGAAGGCT GTCCACAGCA CCTGGGCTGT ACAATCAAGC TTAGAGGAGG CTCCGATTAT 
GAGCTGGCTC GAGTTAAGGA GATCCTCATA TTTATGATCT GTGTAGCATA TCATTCTCAA 
CTAGAAATCT CCTTCCTCAT GGATGAATTT GCCATGCCTC CTACATTAAC GCAAAACCCT 
TCCTTCCACT CTCTGATTGA GGAACAGGAG GATGACGAGG CTGCTCAAGA ACAGCTCAGC 
GGAAGTCCCC TCCCCCGGGA ACCTGACTTC CCTCTGGAGT GTCTGGCCCC TGATGAGAGC 
AGTTCACTGG AATCAAGGAC TGTGTTTGGG AAGAGTGACC AGGAACTTAA AGGTGCTCCA 
AAGGATGCTG TCTCGTTGAA GCAGCAGGAG TTCGCCACAG CAGCCTGCCC GGCCGGCGTC 
CCCTATGCTC TCTTTGCATT GGTCCCAGAA TCCTTGTTGC CACTGCATCT GGGTGACCAA 
CAGGATGCCA GGGGCAGCGA GCAGCCAGAG ACTGTGCAGC AAACGGGTGA GCTACAGGAT 
CCCAAAAGCC AGATGAGAGC CTTTAGAGAC CCTCTGCAGG ATGACACTGG ATTGTATGTC 
ACTGAGGAAG TCACCTCTTC TGAGGATAAG CGGAAGACTT ACTCTTTGGC CTTTAAACAG 
GAATTAAAAG ACGTAATTCT CTGTATCTCC CCAGTGATCA CATTCCGAGA GCCTTTCCTT 
TTAACCGAGA AGGGGATGCG ATGCTCTCCC CGAGACTATT TCGCAGAGCA GCTTTACTGG 
TCTCCTCTCC TCAATAAAGA CTTCAAAGAA ATGGAGAGCA GGCGGAAGAA GCAGCTGCTC 
AGGGATCTCT CCAGCCTCCA GGGCCTGAAC GGAAGTGTGC AGGCCAAGTC TATCCAAGTG 
CTGCCCTCAC ACGAGCTGGT GAGCGCTAGG ATTGCTGAGC ATCTGGGTGA CAGCCAGGGC 
CTGGGCCGGA TGCTGGCTGA CTACCGGGCC AGAGGAGGGA GAATTCAGCA GAATACTTCA 
GACCCTTTTG CTCATTCAAA GGATGCATCA GGTCCTTCAA GTGGCAAGTC AGGAAGCAGA 
ACTGAGGGCG ATGAAGAGAA AGGGCTGATT CCGAGTGACG CGGTATGGTC AACAAAGGTG 
GACTGTCTGA ACCCTGCCAA CCACCAGAGG CTGTGCGTGC TCTTCAGCAG CTCTTCTGCC 
CAGTCCAGCA ACGCGCCCAG CGCCTGCGTC AGTCCTTGGA TTGTAACAAT GGAATTTTAT 
GGAAAGAATG ATCTTACACT GGGAATATTT TTAGAGAGAT ACTGTTTCAG GCCTTCTTAC 
CAGTGTCCTA GCATGTTCTG TGATACCCCC ATGGTACATC ACATTCGGCG CTTTGTCCAT 
GGCCAAGGCT GTGTGCAGAT AATCCTGAAG GAGCTGGATT CTCCAGTACC TGGATATCAG 
CATACAATTC TCACCTATTC CTGGTGCAGA ATCTGCAAAC AGGTAACACC AGTTGTTGCT 
CTTTCCAATG AGTCCTGGTC CATGTCATTT GCCAAATACC TTGAACTTCG GTTTTATGGG 
CACCAGTACA CCCGTAGAGC CAATGCTGAG CCGTGTGGCC ACTCCATCCA CCATGATTAT 
CACCAGTACT TCTCCTATAA CCAGATGGTG GCATCTTTTA GTTATTCTCC CATTCGGCTT 
CTTGAAGTAT GTGTTCCACT CCCCAAAATA TTCATTAAAC GTCAGGCCCC ATTAAAAGTG 
TCCCTTCTTC AAGATCTGAA GGACTTCTTT CAAAAAGTTT CACAAGTATA TCTTGCTGTT 
GATGAAAGAC TTGCATCTTT GAAAACTGAT ACATTTAGCA AAACAAGAGA GGAAAAAATG 
GAAGATATCT TTGCACAAAA AGAGATGGAG GAAGGCGAGT TCAAGAACTG GATTGAGAAA 
ATGCAAGCGA GGCTCCTGTC TTCCTCGGTA GACACCCCTC AGCAGCTGCA GTCGGTCCTG 
GAGTCGCTCA TCGCCAAGAA GCAGAGCCTC TGTGAAGTGC TGCAGGCCTG GAATAACAGG 
TTGCAGGACC TTTTCCAGCA GGAAAAGGGT AGAAAGCGGC CTTCAGTTCC TCCAAGTCCT 
GGAAGACTGA GACAAGGGGA AGAAAGCAAG ATAAGTGCAA TGGATGCATC TCCACGGAAT 
ATTTCTCCAG GACTTCAAAA TGGAGAAAAA GAGGACCGCT TCTTGACAAC CCTCTCTAGT 
CAGGGCCCCA CCTCTGCTGC TCATCTCCAG CTGCCCAGTC CCCCTGAAGT CCTGCCTGAG 
CAGGCAGGGG GGGCGACCCC CGAGCCGGAT GCAGCCAGCA GTTCTGAAGA TGTGTTTGAT 
GGACATTCTC TGGGATCCAC AGACAGCCAG GTGAAGGAAA AATCAACCAT GAAGGCCATC 
TTTGCAAATT TGCTTCCAGG AAATAGCTAT AACCCTATTC CGTTTCCTTT TGATCCAGAT 
AAACACTACT TAATGTACGA ACACGAACGG GTGCCCATTG CCGTCTGTGA GAGGGAGCCC 
AGCTCCATCA TTGCTTTTGC TCTCAGTTGT AAAGAATACC GAAATGCCTT AGAGGAATTG 
TCCAAGGCAA CTCTGCGGAA CAGTGCCGAA GAAGGGCTCC CAGCCAACAG TGCTTCAGAC 
AGCAGACCAA AGGGCGGCAG CCCTGGGAGG CCGCCTGAGC TCGGTGGAGG CTGCCCCAAC 
CGGGCGGCCG AGGCAGAGCC GCAGCCAGCC AAAAAGGCTT CTGGGATGTT GTCCTTCTTC 
CGAGGGACAG CAGGGAAAAG CCCTGATCTC TCTTCCCAGA AGAGAGAGAC CTTACGTGGA 
GCAGATAGTG CTTACTACCA GGTCGGGCAG CCCGGCAAGG AGGGGACGGA GAGTCAAGGC 
ACTGAGCCTC CAGATGAGAT AGATGGAGGA GACGCACAAA AGAAACAGCT CACAAATCCT 
CATGTGGAAC TTCAATTTTC TGACGCTAAC GCCAAGTTTT ACTGCCGGCT CTACTATGCG 
GGAGAGTTTC ACAAGATGCG CGAGGTGATC CTGGGCAGCA GCGAGGAGGA CTTCATCCGC 
TCCCTTTCCC ACTCGGCACC CTGGCAGGCA CGCGGGGGCA AGTCCGGAGC GGCCTTCTAC 
GCCACGGAAG ATGATAGATT CATTTTGAAG CAGATGCCTC GTCTGGAAGT CCAGTCTTTC 
CTCGACTTTG CACCACACTA CTTCAATTAT ATCACAAACG CTGTGCAACA AAAGAGGCCT 
ACTGCACTGG CCAAAATTCT TGGAGTTTAC AGAATTGGTT ATAAGAATTC TCAGAACAAC 
ACTGAGAAGA AGTTAGATCT CCTTGTCATG GAAAATCTTT TCTATGGGAG AAAGATGGCA 
CAGGTTTTTG ACTTGAAGGG TTCACTTAGG AACAGAAATG TAAAAACTGA CACCGGGAAA 
GAGAGCTGTG ACGTGGTCCT GCTGGACGAG AATCTGCTGA AGATGGTCCG GGACAGCCCT 
CTGTACATCC GCTCGCACTC CAAGGCGGTG CTGCGGGCCT CCATCCGCAG CGATGCCCAC 
TTCCTGTCCA GCCACCTCAT CATCGACTAT TCTTTGCTGG TGGGGCGAGA TGATACCAGC 
AATGAGCTTG TCGTTGGGAT TATAGATTAT ATTCGAACGT TTACATGGGA CAAAAAGCTT 
GAGATGGTTG TGAAATCAAC AGGAATTTTA GGAGGACAAG GTAAAATGCC AACTGTGGTC 
TCTCCAGAGT TGTACAGGAC CCGATTTTGT GAAGCGATGG ACAAGTATTT CCTCATGGTA 
CCAGACCACT GGACAGGCTT GGGTCTCAAC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023478288.1
CDS	29..6424
Translation	MATDDKTSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPAFKSAYSSFVNLFRFNKDRAEGEQQSLTGGWPSPQLPSRTQSVRSPIPYKKQLTEELQRRPSAVLAENSLQHPQENTDTRRKTEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCRECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRMDLAVPAPLDAVWIKSYIHGPTGSASITNLSLDRSGSPLVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAVAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTDFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSPSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHVKKIPGGKKFDSMVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDLLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPGEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEEQEDDEAAQEQLSGSPLPREPDFPLECLAPDESSSLESRTVFGKSDQELKGAPKDAVSLKQQEFATAACPAGVPYALFALVPESLLPLHLGDQQDARGSEQPETVQQTGELQDPKSQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSPRDYFAEQLYWSPLLNKDFKEMESRRKKQLLRDLSSLQGLNGSVQAKSIQVLPSHELVSARIAEHLGDSQGLGRMLADYRARGGRIQQNTSDPFAHSKDASGPSSGKSGSRTEGDEEKGLIPSDAVWSTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLLSSSVDTPQQLQSVLESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQGPTSAAHLQLPSPPEVLPEQAGGATPEPDAASSSEDVFDGHSLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSASDSRPKGGSPGRPPELGGGCPNRAAEAEPQPAKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQPGKEGTESQGTEPPDEIDGGDAQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSAPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDSPLYIRSHSKAVLRASIRSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_023622520.1
Organism	Equus caballus(horse)
Definition	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023622520.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGCAGA GAACAGTTTG CAGCACCCCC AGGAGAACAC AGACACAAGA 
AGGAAAACAG AACCTACCTT TGGCGGTCAT GACCCTCGTA CAGCTGTTCA GCTTCGAAGC 
CTCAGCACAG TGTTAAAACG CCTCAAGGAA ATCATGGAGG GGAAAAGCCA GGACAGTGAC 
CTGAAGCAGT ACTGGATGCC GGACAGCCAG TGCAGAGAGT GCTACGACTG CAGCGAGAAG 
TTCACCACCT TCCGGCGCAG GCACCACTGC CGGCTCTGCG GGCAGATTTT CTGCAGCCGC 
TGCTGCAATC AAGAGATCCC TGGGAAGTTT ATGGGCTATA CAGGGGACCT CCGAGCTTGC 
ACATACTGTA GAAAAATAGC CTTAAGTTAT GCTCATTCCA CAGACAGTAA TTCCATTGGG 
GAAGACTTGA ATGCTCTTTC AGATTCTGCT TCCTCTGTGT CTGTACTTGA TCCGAGTGAA 
CCCCGAACAC CTGTTGGGAG TAGGAAAGCC AGCCGGAACA TCTTTTTAGA GGATGATTTT 
GCCTGGCAAA GTTTGATTCA TCCAGACTCC TCAAATACTG CTCTTTCAAC AAGACTTGTA 
TCTGTTCAAG AGGACGCTGG GAAATCTCCT GCTCGAAATA GAATGGACTT GGCAGTGCCT 
GCTCCATTGG ATGCTGTGTG GATTAAATCA TACATACATG GCCCTACTGG GTCAGCCAGC 
ATTACCAACC TCTCACTGGA TCGATCTGGT TCTCCCCTGG TACCTTCATA TGAGACATCT 
GTCAGTCCCC AGGCCAACCG AACATACGTG AGGACAGAGA CCACAGAGGA TGAACGCAAA 
ATTCTTCTGG ACAGCGTCCA GTTAAAAGAC CTGTGGAAGA AAATCTGCCA TCACAGTAGT 
GGAATGGAGT TTCAGGATCA CCGCTACTGG CTGAGGACAC ATCCCAACTG CATCGTAGGG 
AAGGAGTTAG TCAACTGGCT AATCCGAAAT GGGCACATTG CCACAAGGGC ACAAGCTGTA 
GCAATTGGAC AAGCGATGGT GGACGGACGG TGGCTGGACT GCGTGAGCCA CCATGACCAG 
CTCTTCAGGG ATGAGTATGC GCTCTACAGA CCCCTGCAGA GTACAGACTT TTCTGAGACC 
CCTTCTCCGG ACAGTGACTC CGTGAACTCT GTGGAAGGAC ACTCGGAGCC ATCATGGTTT 
AAAGACATAA AGTTTGATGA CAGCGACACA GAGCAGATTG CTGAAGAAGG TGACGATAAT 
TTGACTAACT CTGCCAGTCC TAGCAAGCGC ACGTCAGTCA GCAGTTTCCA GTCCACAGTG 
GACAGTGACT CAGCCGCCTC CATCAGCCTG AACGTGGAGT TGGACAACGT GAACTTCCAC 
ATCAAGAAGC CCTCCAAGTA CCCACACGTG CCCCCTCACC CTGCTGACCA AAAAGAGTAT 
TTGATTTCTG ACAACGGAGG ACAACAACTC TCAATAAGTG ACGCCTTCAT CAAAGAATCC 
TTATTTAATC GTCGAGTTGA GGAGAAATCC AAAGAACTGC CCTTTACGCC TTTGGGCTGG 
CATCATAACA ATCTGGAGCT GCTGCGGGAG GAGAATGGAG AGAAGCAGGC CATGGAGAGG 
TTGCTTTCAG CTAATCATAA CCACATGATG GCACTGCTCC AACAGTTGCT CCATAGTGAG 
TCATTGTCAC CATCTTGGAG GGACATCATT GTATCCCTGG TCTGCCAGGT TGTTCAGACA 
GTCCGACCTG ACGTCAAGAA CCGGGATGAC GACATGGATA TCCGTCAGTT TGTCCACGTC 
AAAAAAATCC CAGGTGGAAA GAAGTTTGAT TCTATGGTTG TCAATGGGTT CGTTTGTACC 
AAGAATATTG CACATAAAAA GATGAATTCT TGTATTAAAA ACCCCAAAAT TCTTCTGTTG 
AAGTGTTCCA TTGAGTATCT CTACAGAGAA GAAACTAAAT TTACTTGCAT TGACCCTATC 
GTGCTTCAGG AAAGGGAATT CTTGAAGAAT TATGTTCAGC GAATAGTGGA TGTTCGACCC 
ACATTGGTTC TAGTTGAGAA AACGGTGTCT CGGATTGCCC AGGACCTGTT GCTGGAACAC 
GGCATCACTT TGGTCATTAA TGTCAAATCA CAAGTTTTAG AACGAATCAG TCGGATGACC 
CAAGGTGATT TAGTGATGTC GATGGACCAG TTGCTCACAA AGCCCCACCT GGGCACCTGT 
CACAAATTTT ACATGCAGAT GTTTCAGCTA CCTGGTGAAC AAACCAAAAC TCTGATGTTT 
TTTGAAGGCT GTCCACAGCA CCTGGGCTGT ACAATCAAGC TTAGAGGAGG CTCCGATTAT 
GAGCTGGCTC GAGTTAAGGA GATCCTCATA TTTATGATCT GTGTAGCATA TCATTCTCAA 
CTAGAAATCT CCTTCCTCAT GGATGAATTT GCCATGCCTC CTACATTAAC GCAAAACCCT 
TCCTTCCACT CTCTGATTGA GGAACAGGAG GATGACGAGG CTGCTCAAGA ACAGCTCAGC 
GGAAGTCCCC TCCCCCGGGA ACCTGACTTC CCTCTGGAGT GTCTGGCCCC TGATGAGAGC 
AGTTCACTGG AATCAAGGAC TGTGTTTGGG AAGAGTGACC AGGAACTTAA AGGTGCTCCA 
AAGGATGCTG TCTCGTTGAA GCAGCAGGAG TTCGCCACAG CAGCCTGCCC GGCCGGCGTC 
CCCTATGCTC TCTTTGCATT GGTCCCAGAA TCCTTGTTGC CACTGCATCT GGGTGACCAA 
CAGGATGCCA GGGGCAGCGA GCAGCCAGAG ACTGTGCAGC AAACGGGTGA GCTACAGGAT 
CCCAAAAGCC AGATGAGAGC CTTTAGAGAC CCTCTGCAGG ATGACACTGG ATTGTATGTC 
ACTGAGGAAG TCACCTCTTC TGAGGATAAG CGGAAGACTT ACTCTTTGGC CTTTAAACAG 
GAATTAAAAG ACGTAATTCT CTGTATCTCC CCAGTGATCA CATTCCGAGA GCCTTTCCTT 
TTAACCGAGA AGGGGATGCG ATGCTCTCCC CGAGACTATT TCGCAGAGCA GCTTTACTGG 
TCTCCTCTCC TCAATAAAGA CTTCAAAGAA ATGGAGAGCA GGCGGAAGAA GCAGCTGCTC 
AGGGATCTCT CCAGCCTCCA GGGCCTGAAC GGAAGTGTGC AGGCCAAGTC TATCCAAGTG 
CTGCCCTCAC ACGAGCTGGT GAGCGCTAGG ATTGCTGAGC ATCTGGGTGA CAGCCAGGGC 
CTGGGCCGGA TGCTGGCTGA CTACCGGGCC AGAGGAGGGA GAATTCAGCA GAATACTTCA 
GACCCTTTTG CTCATTCAAA GGATGCATCA GGTCCTTCAA GTGGCAAGTC AGGAAGCAGA 
ACTGAGGGCG ATGAAGAGAA AGGGCTGATT CCGAGTGACG CGGTATGGTC AACAAAGGTG 
GACTGTCTGA ACCCTGCCAA CCACCAGAGG CTGTGCGTGC TCTTCAGCAG CTCTTCTGCC 
CAGTCCAGCA ACGCGCCCAG CGCCTGCGTC AGTCCTTGGA TTGTAACAAT GGAATTTTAT 
GGAAAGAATG ATCTTACACT GGGAATATTT TTAGAGAGAT ACTGTTTCAG GCCTTCTTAC 
CAGTGTCCTA GCATGTTCTG TGATACCCCC ATGGTACATC ACATTCGGCG CTTTGTCCAT 
GGCCAAGGCT GTGTGCAGAT AATCCTGAAG GAGCTGGATT CTCCAGTACC TGGATATCAG 
CATACAATTC TCACCTATTC CTGGTGCAGA ATCTGCAAAC AGGTAACACC AGTTGTTGCT 
CTTTCCAATG AGTCCTGGTC CATGTCATTT GCCAAATACC TTGAACTTCG GTTTTATGGG 
CACCAGTACA CCCGTAGAGC CAATGCTGAG CCGTGTGGCC ACTCCATCCA CCATGATTAT 
CACCAGTACT TCTCCTATAA CCAGATGGTG GCATCTTTTA GTTATTCTCC CATTCGGCTT 
CTTGAAGTAT GTGTTCCACT CCCCAAAATA TTCATTAAAC GTCAGGCCCC ATTAAAAGTG 
TCCCTTCTTC AAGATCTGAA GGACTTCTTT CAAAAAGTTT CACAAGTATA TCTTGCTGTT 
GATGAAAGAC TTGCATCTTT GAAAACTGAT ACATTTAGCA AAACAAGAGA GGAAAAAATG 
GAAGATATCT TTGCACAAAA AGAGATGGAG GAAGGCGAGT TCAAGAACTG GATTGAGAAA 
ATGCAAGCGA GGCTCCTGTC TTCCTCGGTA GACACCCCTC AGCAGCTGCA GTCGGTCCTG 
GAGTCGCTCA TCGCCAAGAA GCAGAGCCTC TGTGAAGTGC TGCAGGCCTG GAATAACAGG 
TTGCAGGACC TTTTCCAGCA GGAAAAGGGT AGAAAGCGGC CTTCAGTTCC TCCAAGTCCT 
GGAAGACTGA GACAAGGGGA AGAAAGCAAG ATAAGTGCAA TGGATGCATC TCCACGGAAT 
ATTTCTCCAG GACTTCAAAA TGGAGAAAAA GAGGACCGCT TCTTGACAAC CCTCTCTAGT 
CAGGGCCCCA CCTCTGCTGC TCATCTCCAG CTGCCCAGTC CCCCTGAAGT CCTGCCTGAG 
CAGGCAGGGG GGGCGACCCC CGAGCCGGAT GCAGCCAGCA GTTCTGAAGA TGTGTTTGAT 
GGACATTCTC TGGGATCCAC AGACAGCCAG GTGAAGGAAA AATCAACCAT GAAGGCCATC 
TTTGCAAATT TGCTTCCAGG AAATAGCTAT AACCCTATTC CGTTTCCTTT TGATCCAGAT 
AAACACTACT TAATGTACGA ACACGAACGG GTGCCCATTG CCGTCTGTGA GAGGGAGCCC 
AGCTCCATCA TTGCTTTTGC TCTCAGTTGT AAAGAATACC GAAATGCCTT AGAGGAATTG 
TCCAAGGCAA CTCTGCGGAA CAGTGCCGAA GAAGGGCTCC CAGCCAACAG TGCTTCAGAC 
AGCAGACCAA AGGGCGGCAG CCCTGGGAGG CCGCCTGAGC TCGGTGGAGG CTGCCCCAAC 
CGGGCGGCCG AGGCAGAGCC GCAGCCAGCC AAAAAGGCTT CTGGGATGTT GTCCTTCTTC 
CGAGGGACAG CAGGGAAAAG CCCTGATCTC TCTTCCCAGA AGAGAGAGAC CTTACGTGGA 
GCAGATAGTG CTTACTACCA GGTCGGGCAG CCCGGCAAGG AGGGGACGGA GAGTCAAGGC 
ACTGAGCCTC CAGATGAGAT AGATGGAGGA GACGCACAAA AGAAACAGCT CACAAATCCT 
CATGTGGAAC TTCAATTTTC TGACGCTAAC GCCAAGTTTT ACTGCCGGCT CTACTATGCG 
GGAGAGTTTC ACAAGATGCG CGAGGTGATC CTGGGCAGCA GCGAGGAGGA CTTCATCCGC 
TCCCTTTCCC ACTCGGCACC CTGGCAGGCA CGCGGGGGCA AGTCCGGAGC GGCCTTCTAC 
GCCACGGAAG ATGATAGATT CATTTTGAAG CAGATGCCTC GTCTGGAAGT CCAGTCTTTC 
CTCGACTTTG CACCACACTA CTTCAATTAT ATCACAAACG CTGTGCAACA AAAGAGGCCT 
ACTGCACTGG CCAAAATTCT TGGAGTTTAC AGAATTGGTT ATAAGAATTC TCAGAACAAC 
ACTGAGAAGA AGTTAGATCT CCTTGTCATG GAAAATCTTT TCTATGGGAG AAAGATGGCA 
CAGGTTTTTG ACTTGAAGGG TTCACTTAGG AACAGAAATG TAAAAACTGA CACCGGGAAA 
GAGAGCTGTG ACGTGGTCCT GCTGGACGAG AATCTGCTGA AGATGGTCCG GGACAGCCCT 
CTGTACATCC GCTCGCACTC CAAGGCGGTG CTGCGGGCCT CCATCCGCAG CGATGCCCAC 
TTCCTGTCCA GCCACCTCAT CATCGACTAT TCTTTGCTGG TGGGGCGAGA TGATACCAGC 
AATGAGCTTG TCGTTGGGAT TATAGATTAT ATTCGAACGT TTACATGGGA CAAAAAGCTT 
GAGATGGTTG TGAAATCAAC AGGAATTTTA GGAGGACAAG GTAAAATGCC AACTGTGGTC 
TCTCCAGAGT TGTACAGGAC CCGATTTTGT GAAGCGATGG ACAAGTATTT CCTCATGGTA 
CCAGACCACT GGACAGGCTT GGGTCTCAAC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_023622521.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023478289.1 Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X5, mRNA.

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CloneID	OHb52470
Clone ID Related Accession (Same CDS sequence)	XM_023622521.1
Accession Version	XM_023622521.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGAGAA CAGTTTGCAG CACCCCCAGG AGAACACAGA CACAAGAAGG 
AAAACAGAAC CTACCTTTGG CGGTCATGAC CCTCGTACAG CTGTTCAGCT TCGAAGCCTC 
AGCACAGTGT TAAAACGCCT CAAGGAAATC ATGGAGGGGA AAAGCCAGGA CAGTGACCTG 
AAGCAGTACT GGATGCCGGA CAGCCAGTGC AGAGAGTGCT ACGACTGCAG CGAGAAGTTC 
ACCACCTTCC GGCGCAGGCA CCACTGCCGG CTCTGCGGGC AGATTTTCTG CAGCCGCTGC 
TGCAATCAAG AGATCCCTGG GAAGTTTATG GGCTATACAG GGGACCTCCG AGCTTGCACA 
TACTGTAGAA AAATAGCCTT AAGTTATGCT CATTCCACAG ACAGTAATTC CATTGGGGAA 
GACTTGAATG CTCTTTCAGA TTCTGCTTCC TCTGTGTCTG TACTTGATCC GAGTGAACCC 
CGAACACCTG TTGGGAGTAG GAAAGCCAGC CGGAACATCT TTTTAGAGGA TGATTTTGCC 
TGGCAAAGTT TGATTCATCC AGACTCCTCA AATACTGCTC TTTCAACAAG ACTTGTATCT 
GTTCAAGAGG ACGCTGGGAA ATCTCCTGCT CGAAATAGAA TGGACTTGGC AGTGCCTGCT 
CCATTGGATG CTGTGTGGAT TAAATCATAC ATACATGGCC CTACTGGGTC AGCCAGCATT 
ACCAACCTCT CACTGGATCG ATCTGGTTCT CCCCTGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CCAACCGAAC ATACGTGAGG ACAGAGACCA CAGAGGATGA ACGCAAAATT 
CTTCTGGACA GCGTCCAGTT AAAAGACCTG TGGAAGAAAA TCTGCCATCA CAGTAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGGACACATC CCAACTGCAT CGTAGGGAAG 
GAGTTAGTCA ACTGGCTAAT CCGAAATGGG CACATTGCCA CAAGGGCACA AGCTGTAGCA 
ATTGGACAAG CGATGGTGGA CGGACGGTGG CTGGACTGCG TGAGCCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT CTACAGACCC CTGCAGAGTA CAGACTTTTC TGAGACCCCT 
TCTCCGGACA GTGACTCCGT GAACTCTGTG GAAGGACACT CGGAGCCATC ATGGTTTAAA 
GACATAAAGT TTGATGACAG CGACACAGAG CAGATTGCTG AAGAAGGTGA CGATAATTTG 
ACTAACTCTG CCAGTCCTAG CAAGCGCACG TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCCTCCAT CAGCCTGAAC GTGGAGTTGG ACAACGTGAA CTTCCACATC 
AAGAAGCCCT CCAAGTACCC ACACGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA ACGGAGGACA ACAACTCTCA ATAAGTGACG CCTTCATCAA AGAATCCTTA 
TTTAATCGTC GAGTTGAGGA GAAATCCAAA GAACTGCCCT TTACGCCTTT GGGCTGGCAT 
CATAACAATC TGGAGCTGCT GCGGGAGGAG AATGGAGAGA AGCAGGCCAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCA CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA 
TTGTCACCAT CTTGGAGGGA CATCATTGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGAACCG GGATGACGAC ATGGATATCC GTCAGTTTGT CCACGTCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT ATGGTTGTCA ATGGGTTCGT TTGTACCAAG 
AATATTGCAC ATAAAAAGAT GAATTCTTGT ATTAAAAACC CCAAAATTCT TCTGTTGAAG 
TGTTCCATTG AGTATCTCTA CAGAGAAGAA ACTAAATTTA CTTGCATTGA CCCTATCGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAGCGAA TAGTGGATGT TCGACCCACA 
TTGGTTCTAG TTGAGAAAAC GGTGTCTCGG ATTGCCCAGG ACCTGTTGCT GGAACACGGC 
ATCACTTTGG TCATTAATGT CAAATCACAA GTTTTAGAAC GAATCAGTCG GATGACCCAA 
GGTGATTTAG TGATGTCGAT GGACCAGTTG CTCACAAAGC CCCACCTGGG CACCTGTCAC 
AAATTTTACA TGCAGATGTT TCAGCTACCT GGTGAACAAA CCAAAACTCT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC CGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTCATATTT ATGATCTGTG TAGCATATCA TTCTCAACTA 
GAAATCTCCT TCCTCATGGA TGAATTTGCC ATGCCTCCTA CATTAACGCA AAACCCTTCC 
TTCCACTCTC TGATTGAGGA ACAGGAGGAT GACGAGGCTG CTCAAGAACA GCTCAGCGGA 
AGTCCCCTCC CCCGGGAACC TGACTTCCCT CTGGAGTGTC TGGCCCCTGA TGAGAGCAGT 
TCACTGGAAT CAAGGACTGT GTTTGGGAAG AGTGACCAGG AACTTAAAGG TGCTCCAAAG 
GATGCTGTCT CGTTGAAGCA GCAGGAGTTC GCCACAGCAG CCTGCCCGGC CGGCGTCCCC 
TATGCTCTCT TTGCATTGGT CCCAGAATCC TTGTTGCCAC TGCATCTGGG TGACCAACAG 
GATGCCAGGG GCAGCGAGCA GCCAGAGACT GTGCAGCAAA CGGGTGAGCT ACAGGATCCC 
AAAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACTGGATT GTATGTCACT 
GAGGAAGTCA CCTCTTCTGA GGATAAGCGG AAGACTTACT CTTTGGCCTT TAAACAGGAA 
TTAAAAGACG TAATTCTCTG TATCTCCCCA GTGATCACAT TCCGAGAGCC TTTCCTTTTA 
ACCGAGAAGG GGATGCGATG CTCTCCCCGA GACTATTTCG CAGAGCAGCT TTACTGGTCT 
CCTCTCCTCA ATAAAGACTT CAAAGAAATG GAGAGCAGGC GGAAGAAGCA GCTGCTCAGG 
GATCTCTCCA GCCTCCAGGG CCTGAACGGA AGTGTGCAGG CCAAGTCTAT CCAAGTGCTG 
CCCTCACACG AGCTGGTGAG CGCTAGGATT GCTGAGCATC TGGGTGACAG CCAGGGCCTG 
GGCCGGATGC TGGCTGACTA CCGGGCCAGA GGAGGGAGAA TTCAGCAGAA TACTTCAGAC 
CCTTTTGCTC ATTCAAAGGA TGCATCAGGT CCTTCAAGTG GCAAGTCAGG AAGCAGAACT 
GAGGGCGATG AAGAGAAAGG GCTGATTCCG AGTGACGCGG TATGGTCAAC AAAGGTGGAC 
TGTCTGAACC CTGCCAACCA CCAGAGGCTG TGCGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAACG CGCCCAGCGC CTGCGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAGAATGATC TTACACTGGG AATATTTTTA GAGAGATACT GTTTCAGGCC TTCTTACCAG 
TGTCCTAGCA TGTTCTGTGA TACCCCCATG GTACATCACA TTCGGCGCTT TGTCCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTCA CCTATTCCTG GTGCAGAATC TGCAAACAGG TAACACCAGT TGTTGCTCTT 
TCCAATGAGT CCTGGTCCAT GTCATTTGCC AAATACCTTG AACTTCGGTT TTATGGGCAC 
CAGTACACCC GTAGAGCCAA TGCTGAGCCG TGTGGCCACT CCATCCACCA TGATTATCAC 
CAGTACTTCT CCTATAACCA GATGGTGGCA TCTTTTAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTATGTG TTCCACTCCC CAAAATATTC ATTAAACGTC AGGCCCCATT AAAAGTGTCC 
CTTCTTCAAG ATCTGAAGGA CTTCTTTCAA AAAGTTTCAC AAGTATATCT TGCTGTTGAT 
GAAAGACTTG CATCTTTGAA AACTGATACA TTTAGCAAAA CAAGAGAGGA AAAAATGGAA 
GATATCTTTG CACAAAAAGA GATGGAGGAA GGCGAGTTCA AGAACTGGAT TGAGAAAATG 
CAAGCGAGGC TCCTGTCTTC CTCGGTAGAC ACCCCTCAGC AGCTGCAGTC GGTCCTGGAG 
TCGCTCATCG CCAAGAAGCA GAGCCTCTGT GAAGTGCTGC AGGCCTGGAA TAACAGGTTG 
CAGGACCTTT TCCAGCAGGA AAAGGGTAGA AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGGGAAGA AAGCAAGATA AGTGCAATGG ATGCATCTCC ACGGAATATT 
TCTCCAGGAC TTCAAAATGG AGAAAAAGAG GACCGCTTCT TGACAACCCT CTCTAGTCAG 
GGCCCCACCT CTGCTGCTCA TCTCCAGCTG CCCAGTCCCC CTGAAGTCCT GCCTGAGCAG 
GCAGGGGGGG CGACCCCCGA GCCGGATGCA GCCAGCAGTT CTGAAGATGT GTTTGATGGA 
CATTCTCTGG GATCCACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA GGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCGT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTACGAACA CGAACGGGTG CCCATTGCCG TCTGTGAGAG GGAGCCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGAA ATGCCTTAGA GGAATTGTCC 
AAGGCAACTC TGCGGAACAG TGCCGAAGAA GGGCTCCCAG CCAACAGTGC TTCAGACAGC 
AGACCAAAGG GCGGCAGCCC TGGGAGGCCG CCTGAGCTCG GTGGAGGCTG CCCCAACCGG 
GCGGCCGAGG CAGAGCCGCA GCCAGCCAAA AAGGCTTCTG GGATGTTGTC CTTCTTCCGA 
GGGACAGCAG GGAAAAGCCC TGATCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA 
GATAGTGCTT ACTACCAGGT CGGGCAGCCC GGCAAGGAGG GGACGGAGAG TCAAGGCACT 
GAGCCTCCAG ATGAGATAGA TGGAGGAGAC GCACAAAAGA AACAGCTCAC AAATCCTCAT 
GTGGAACTTC AATTTTCTGA CGCTAACGCC AAGTTTTACT GCCGGCTCTA CTATGCGGGA 
GAGTTTCACA AGATGCGCGA GGTGATCCTG GGCAGCAGCG AGGAGGACTT CATCCGCTCC 
CTTTCCCACT CGGCACCCTG GCAGGCACGC GGGGGCAAGT CCGGAGCGGC CTTCTACGCC 
ACGGAAGATG ATAGATTCAT TTTGAAGCAG ATGCCTCGTC TGGAAGTCCA GTCTTTCCTC 
GACTTTGCAC CACACTACTT CAATTATATC ACAAACGCTG TGCAACAAAA GAGGCCTACT 
GCACTGGCCA AAATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT 
GAGAAGAAGT TAGATCTCCT TGTCATGGAA AATCTTTTCT ATGGGAGAAA GATGGCACAG 
GTTTTTGACT TGAAGGGTTC ACTTAGGAAC AGAAATGTAA AAACTGACAC CGGGAAAGAG 
AGCTGTGACG TGGTCCTGCT GGACGAGAAT CTGCTGAAGA TGGTCCGGGA CAGCCCTCTG 
TACATCCGCT CGCACTCCAA GGCGGTGCTG CGGGCCTCCA TCCGCAGCGA TGCCCACTTC 
CTGTCCAGCC ACCTCATCAT CGACTATTCT TTGCTGGTGG GGCGAGATGA TACCAGCAAT 
GAGCTTGTCG TTGGGATTAT AGATTATATT CGAACGTTTA CATGGGACAA AAAGCTTGAG 
ATGGTTGTGA AATCAACAGG AATTTTAGGA GGACAAGGTA AAATGCCAAC TGTGGTCTCT 
CCAGAGTTGT ACAGGACCCG ATTTTGTGAA GCGATGGACA AGTATTTCCT CATGGTACCA 
GACCACTGGA CAGGCTTGGG TCTCAACTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023478289.1
CDS	114..6506
Translation	MATDDKTSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPAFKSAYSSFVNLFRFNKDRAEGEQQSLTGGWPSPQLPSRTQSVRSPIPYKKQLTEELQRRPSAVLENSLQHPQENTDTRRKTEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCRECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRMDLAVPAPLDAVWIKSYIHGPTGSASITNLSLDRSGSPLVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAVAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTDFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSPSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHVKKIPGGKKFDSMVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDLLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPGEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEEQEDDEAAQEQLSGSPLPREPDFPLECLAPDESSSLESRTVFGKSDQELKGAPKDAVSLKQQEFATAACPAGVPYALFALVPESLLPLHLGDQQDARGSEQPETVQQTGELQDPKSQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSPRDYFAEQLYWSPLLNKDFKEMESRRKKQLLRDLSSLQGLNGSVQAKSIQVLPSHELVSARIAEHLGDSQGLGRMLADYRARGGRIQQNTSDPFAHSKDASGPSSGKSGSRTEGDEEKGLIPSDAVWSTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLLSSSVDTPQQLQSVLESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQGPTSAAHLQLPSPPEVLPEQAGGATPEPDAASSSEDVFDGHSLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSASDSRPKGGSPGRPPELGGGCPNRAAEAEPQPAKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQPGKEGTESQGTEPPDEIDGGDAQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSAPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDSPLYIRSHSKAVLRASIRSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_023622521.1
Organism	Equus caballus(horse)
Definition	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023622521.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGAGAA CAGTTTGCAG CACCCCCAGG AGAACACAGA CACAAGAAGG 
AAAACAGAAC CTACCTTTGG CGGTCATGAC CCTCGTACAG CTGTTCAGCT TCGAAGCCTC 
AGCACAGTGT TAAAACGCCT CAAGGAAATC ATGGAGGGGA AAAGCCAGGA CAGTGACCTG 
AAGCAGTACT GGATGCCGGA CAGCCAGTGC AGAGAGTGCT ACGACTGCAG CGAGAAGTTC 
ACCACCTTCC GGCGCAGGCA CCACTGCCGG CTCTGCGGGC AGATTTTCTG CAGCCGCTGC 
TGCAATCAAG AGATCCCTGG GAAGTTTATG GGCTATACAG GGGACCTCCG AGCTTGCACA 
TACTGTAGAA AAATAGCCTT AAGTTATGCT CATTCCACAG ACAGTAATTC CATTGGGGAA 
GACTTGAATG CTCTTTCAGA TTCTGCTTCC TCTGTGTCTG TACTTGATCC GAGTGAACCC 
CGAACACCTG TTGGGAGTAG GAAAGCCAGC CGGAACATCT TTTTAGAGGA TGATTTTGCC 
TGGCAAAGTT TGATTCATCC AGACTCCTCA AATACTGCTC TTTCAACAAG ACTTGTATCT 
GTTCAAGAGG ACGCTGGGAA ATCTCCTGCT CGAAATAGAA TGGACTTGGC AGTGCCTGCT 
CCATTGGATG CTGTGTGGAT TAAATCATAC ATACATGGCC CTACTGGGTC AGCCAGCATT 
ACCAACCTCT CACTGGATCG ATCTGGTTCT CCCCTGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CCAACCGAAC ATACGTGAGG ACAGAGACCA CAGAGGATGA ACGCAAAATT 
CTTCTGGACA GCGTCCAGTT AAAAGACCTG TGGAAGAAAA TCTGCCATCA CAGTAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGGACACATC CCAACTGCAT CGTAGGGAAG 
GAGTTAGTCA ACTGGCTAAT CCGAAATGGG CACATTGCCA CAAGGGCACA AGCTGTAGCA 
ATTGGACAAG CGATGGTGGA CGGACGGTGG CTGGACTGCG TGAGCCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT CTACAGACCC CTGCAGAGTA CAGACTTTTC TGAGACCCCT 
TCTCCGGACA GTGACTCCGT GAACTCTGTG GAAGGACACT CGGAGCCATC ATGGTTTAAA 
GACATAAAGT TTGATGACAG CGACACAGAG CAGATTGCTG AAGAAGGTGA CGATAATTTG 
ACTAACTCTG CCAGTCCTAG CAAGCGCACG TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCCTCCAT CAGCCTGAAC GTGGAGTTGG ACAACGTGAA CTTCCACATC 
AAGAAGCCCT CCAAGTACCC ACACGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA ACGGAGGACA ACAACTCTCA ATAAGTGACG CCTTCATCAA AGAATCCTTA 
TTTAATCGTC GAGTTGAGGA GAAATCCAAA GAACTGCCCT TTACGCCTTT GGGCTGGCAT 
CATAACAATC TGGAGCTGCT GCGGGAGGAG AATGGAGAGA AGCAGGCCAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCA CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA 
TTGTCACCAT CTTGGAGGGA CATCATTGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGAACCG GGATGACGAC ATGGATATCC GTCAGTTTGT CCACGTCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT ATGGTTGTCA ATGGGTTCGT TTGTACCAAG 
AATATTGCAC ATAAAAAGAT GAATTCTTGT ATTAAAAACC CCAAAATTCT TCTGTTGAAG 
TGTTCCATTG AGTATCTCTA CAGAGAAGAA ACTAAATTTA CTTGCATTGA CCCTATCGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAGCGAA TAGTGGATGT TCGACCCACA 
TTGGTTCTAG TTGAGAAAAC GGTGTCTCGG ATTGCCCAGG ACCTGTTGCT GGAACACGGC 
ATCACTTTGG TCATTAATGT CAAATCACAA GTTTTAGAAC GAATCAGTCG GATGACCCAA 
GGTGATTTAG TGATGTCGAT GGACCAGTTG CTCACAAAGC CCCACCTGGG CACCTGTCAC 
AAATTTTACA TGCAGATGTT TCAGCTACCT GGTGAACAAA CCAAAACTCT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC CGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTCATATTT ATGATCTGTG TAGCATATCA TTCTCAACTA 
GAAATCTCCT TCCTCATGGA TGAATTTGCC ATGCCTCCTA CATTAACGCA AAACCCTTCC 
TTCCACTCTC TGATTGAGGA ACAGGAGGAT GACGAGGCTG CTCAAGAACA GCTCAGCGGA 
AGTCCCCTCC CCCGGGAACC TGACTTCCCT CTGGAGTGTC TGGCCCCTGA TGAGAGCAGT 
TCACTGGAAT CAAGGACTGT GTTTGGGAAG AGTGACCAGG AACTTAAAGG TGCTCCAAAG 
GATGCTGTCT CGTTGAAGCA GCAGGAGTTC GCCACAGCAG CCTGCCCGGC CGGCGTCCCC 
TATGCTCTCT TTGCATTGGT CCCAGAATCC TTGTTGCCAC TGCATCTGGG TGACCAACAG 
GATGCCAGGG GCAGCGAGCA GCCAGAGACT GTGCAGCAAA CGGGTGAGCT ACAGGATCCC 
AAAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACTGGATT GTATGTCACT 
GAGGAAGTCA CCTCTTCTGA GGATAAGCGG AAGACTTACT CTTTGGCCTT TAAACAGGAA 
TTAAAAGACG TAATTCTCTG TATCTCCCCA GTGATCACAT TCCGAGAGCC TTTCCTTTTA 
ACCGAGAAGG GGATGCGATG CTCTCCCCGA GACTATTTCG CAGAGCAGCT TTACTGGTCT 
CCTCTCCTCA ATAAAGACTT CAAAGAAATG GAGAGCAGGC GGAAGAAGCA GCTGCTCAGG 
GATCTCTCCA GCCTCCAGGG CCTGAACGGA AGTGTGCAGG CCAAGTCTAT CCAAGTGCTG 
CCCTCACACG AGCTGGTGAG CGCTAGGATT GCTGAGCATC TGGGTGACAG CCAGGGCCTG 
GGCCGGATGC TGGCTGACTA CCGGGCCAGA GGAGGGAGAA TTCAGCAGAA TACTTCAGAC 
CCTTTTGCTC ATTCAAAGGA TGCATCAGGT CCTTCAAGTG GCAAGTCAGG AAGCAGAACT 
GAGGGCGATG AAGAGAAAGG GCTGATTCCG AGTGACGCGG TATGGTCAAC AAAGGTGGAC 
TGTCTGAACC CTGCCAACCA CCAGAGGCTG TGCGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAACG CGCCCAGCGC CTGCGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAGAATGATC TTACACTGGG AATATTTTTA GAGAGATACT GTTTCAGGCC TTCTTACCAG 
TGTCCTAGCA TGTTCTGTGA TACCCCCATG GTACATCACA TTCGGCGCTT TGTCCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTCA CCTATTCCTG GTGCAGAATC TGCAAACAGG TAACACCAGT TGTTGCTCTT 
TCCAATGAGT CCTGGTCCAT GTCATTTGCC AAATACCTTG AACTTCGGTT TTATGGGCAC 
CAGTACACCC GTAGAGCCAA TGCTGAGCCG TGTGGCCACT CCATCCACCA TGATTATCAC 
CAGTACTTCT CCTATAACCA GATGGTGGCA TCTTTTAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTATGTG TTCCACTCCC CAAAATATTC ATTAAACGTC AGGCCCCATT AAAAGTGTCC 
CTTCTTCAAG ATCTGAAGGA CTTCTTTCAA AAAGTTTCAC AAGTATATCT TGCTGTTGAT 
GAAAGACTTG CATCTTTGAA AACTGATACA TTTAGCAAAA CAAGAGAGGA AAAAATGGAA 
GATATCTTTG CACAAAAAGA GATGGAGGAA GGCGAGTTCA AGAACTGGAT TGAGAAAATG 
CAAGCGAGGC TCCTGTCTTC CTCGGTAGAC ACCCCTCAGC AGCTGCAGTC GGTCCTGGAG 
TCGCTCATCG CCAAGAAGCA GAGCCTCTGT GAAGTGCTGC AGGCCTGGAA TAACAGGTTG 
CAGGACCTTT TCCAGCAGGA AAAGGGTAGA AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGGGAAGA AAGCAAGATA AGTGCAATGG ATGCATCTCC ACGGAATATT 
TCTCCAGGAC TTCAAAATGG AGAAAAAGAG GACCGCTTCT TGACAACCCT CTCTAGTCAG 
GGCCCCACCT CTGCTGCTCA TCTCCAGCTG CCCAGTCCCC CTGAAGTCCT GCCTGAGCAG 
GCAGGGGGGG CGACCCCCGA GCCGGATGCA GCCAGCAGTT CTGAAGATGT GTTTGATGGA 
CATTCTCTGG GATCCACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA GGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCGT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTACGAACA CGAACGGGTG CCCATTGCCG TCTGTGAGAG GGAGCCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGAA ATGCCTTAGA GGAATTGTCC 
AAGGCAACTC TGCGGAACAG TGCCGAAGAA GGGCTCCCAG CCAACAGTGC TTCAGACAGC 
AGACCAAAGG GCGGCAGCCC TGGGAGGCCG CCTGAGCTCG GTGGAGGCTG CCCCAACCGG 
GCGGCCGAGG CAGAGCCGCA GCCAGCCAAA AAGGCTTCTG GGATGTTGTC CTTCTTCCGA 
GGGACAGCAG GGAAAAGCCC TGATCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA 
GATAGTGCTT ACTACCAGGT CGGGCAGCCC GGCAAGGAGG GGACGGAGAG TCAAGGCACT 
GAGCCTCCAG ATGAGATAGA TGGAGGAGAC GCACAAAAGA AACAGCTCAC AAATCCTCAT 
GTGGAACTTC AATTTTCTGA CGCTAACGCC AAGTTTTACT GCCGGCTCTA CTATGCGGGA 
GAGTTTCACA AGATGCGCGA GGTGATCCTG GGCAGCAGCG AGGAGGACTT CATCCGCTCC 
CTTTCCCACT CGGCACCCTG GCAGGCACGC GGGGGCAAGT CCGGAGCGGC CTTCTACGCC 
ACGGAAGATG ATAGATTCAT TTTGAAGCAG ATGCCTCGTC TGGAAGTCCA GTCTTTCCTC 
GACTTTGCAC CACACTACTT CAATTATATC ACAAACGCTG TGCAACAAAA GAGGCCTACT 
GCACTGGCCA AAATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT 
GAGAAGAAGT TAGATCTCCT TGTCATGGAA AATCTTTTCT ATGGGAGAAA GATGGCACAG 
GTTTTTGACT TGAAGGGTTC ACTTAGGAAC AGAAATGTAA AAACTGACAC CGGGAAAGAG 
AGCTGTGACG TGGTCCTGCT GGACGAGAAT CTGCTGAAGA TGGTCCGGGA CAGCCCTCTG 
TACATCCGCT CGCACTCCAA GGCGGTGCTG CGGGCCTCCA TCCGCAGCGA TGCCCACTTC 
CTGTCCAGCC ACCTCATCAT CGACTATTCT TTGCTGGTGG GGCGAGATGA TACCAGCAAT 
GAGCTTGTCG TTGGGATTAT AGATTATATT CGAACGTTTA CATGGGACAA AAAGCTTGAG 
ATGGTTGTGA AATCAACAGG AATTTTAGGA GGACAAGGTA AAATGCCAAC TGTGGTCTCT 
CCAGAGTTGT ACAGGACCCG ATTTTGTGAA GCGATGGACA AGTATTTCCT CATGGTACCA 
GACCACTGGA CAGGCTTGGG TCTCAACTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_023622522.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023478290.1 Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X6, mRNA.

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CloneID	OHb52471
Clone ID Related Accession (Same CDS sequence)	XM_023622522.1
Accession Version	XM_023622522.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGCAGA GAACAGTTTG CAGCACCCCC AGGAGAACAC AGACACAAGA 
AGGAAAACAG AACCTACCTT TGGCGGTCAT GACCCTCGTA CAGCTGTTCA GCTTCGAAGC 
CTCAGCACAG TGTTAAAACG CCTCAAGGAA ATCATGGAGG GGAAAAGCCA GGACAGTGAC 
CTGAAGCAGT ACTGGATGCC GGACAGCCAG TGCAGAGAGT GCTACGACTG CAGCGAGAAG 
TTCACCACCT TCCGGCGCAG GCACCACTGC CGGCTCTGCG GGCAGATTTT CTGCAGCCGC 
TGCTGCAATC AAGAGATCCC TGGGAAGTTT ATGGGCTATA CAGGGGACCT CCGAGCTTGC 
ACATACTGTA GAAAAATAGC CTTAAGTTAT GCTCATTCCA CAGACAGTAA TTCCATTGGG 
GAAGACTTGA ATGCTCTTTC AGATTCTGCT TCCTCTGTGT CTGTACTTGA TCCGAGTGAA 
CCCCGAACAC CTGTTGGGAG TAGGAAAGCC AGCCGGAACA TCTTTTTAGA GGATGATTTT 
GCCTGGCAAA GTTTGATTCA TCCAGACTCC TCAAATACTG CTCTTTCAAC AAGACTTGTA 
TCTGTTCAAG AGGACGCTGG GAAATCTCCT GCTCGAAATA GAATGGACTT GGCAGTGCCT 
GCTCCATTGG ATGCTGTGTG GATTAAATCA TACATACATG GCCCTACTGG GTCAGCCAGC 
ATTACCAACC TCTCACTGGA TCGATCTGGT TCTCCCCTGG TACCTTCATA TGAGACATCT 
GTCAGTCCCC AGGCCAACCG AACATACGTG AGGACAGAGA CCACAGAGGA TGAACGCAAA 
ATTCTTCTGG ACAGCGTCCA GTTAAAAGAC CTGTGGAAGA AAATCTGCCA TCACAGTAGT 
GGAATGGAGT TTCAGGATCA CCGCTACTGG CTGAGGACAC ATCCCAACTG CATCGTAGGG 
AAGGAGTTAG TCAACTGGCT AATCCGAAAT GGGCACATTG CCACAAGGGC ACAAGCTGTA 
GCAATTGGAC AAGCGATGGT GGACGGACGG TGGCTGGACT GCGTGAGCCA CCATGACCAG 
CTCTTCAGGG ATGAGTATGC GCTCTACAGA CCCCTGCAGA GTACAGACTT TTCTGAGACC 
CCTTCTCCGG ACAGTGACTC CGTGAACTCT GTGGAAGGAC ACTCGGAGCC ATCATGGTTT 
AAAGACATAA AGTTTGATGA CAGCGACACA GAGCAGATTG CTGAAGAAGA CTCTGCCAGT 
CCTAGCAAGC GCACGTCAGT CAGCAGTTTC CAGTCCACAG TGGACAGTGA CTCAGCCGCC 
TCCATCAGCC TGAACGTGGA GTTGGACAAC GTGAACTTCC ACATCAAGAA GCCCTCCAAG 
TACCCACACG TGCCCCCTCA CCCTGCTGAC CAAAAAGAGT ATTTGATTTC TGACAACGGA 
GGACAACAAC TCTCAATAAG TGACGCCTTC ATCAAAGAAT CCTTATTTAA TCGTCGAGTT 
GAGGAGAAAT CCAAAGAACT GCCCTTTACG CCTTTGGGCT GGCATCATAA CAATCTGGAG 
CTGCTGCGGG AGGAGAATGG AGAGAAGCAG GCCATGGAGA GGTTGCTTTC AGCTAATCAT 
AACCACATGA TGGCACTGCT CCAACAGTTG CTCCATAGTG AGTCATTGTC ACCATCTTGG 
AGGGACATCA TTGTATCCCT GGTCTGCCAG GTTGTTCAGA CAGTCCGACC TGACGTCAAG 
AACCGGGATG ACGACATGGA TATCCGTCAG TTTGTCCACG TCAAAAAAAT CCCAGGTGGA 
AAGAAGTTTG ATTCTATGGT TGTCAATGGG TTCGTTTGTA CCAAGAATAT TGCACATAAA 
AAGATGAATT CTTGTATTAA AAACCCCAAA ATTCTTCTGT TGAAGTGTTC CATTGAGTAT 
CTCTACAGAG AAGAAACTAA ATTTACTTGC ATTGACCCTA TCGTGCTTCA GGAAAGGGAA 
TTCTTGAAGA ATTATGTTCA GCGAATAGTG GATGTTCGAC CCACATTGGT TCTAGTTGAG 
AAAACGGTGT CTCGGATTGC CCAGGACCTG TTGCTGGAAC ACGGCATCAC TTTGGTCATT 
AATGTCAAAT CACAAGTTTT AGAACGAATC AGTCGGATGA CCCAAGGTGA TTTAGTGATG 
TCGATGGACC AGTTGCTCAC AAAGCCCCAC CTGGGCACCT GTCACAAATT TTACATGCAG 
ATGTTTCAGC TACCTGGTGA ACAAACCAAA ACTCTGATGT TTTTTGAAGG CTGTCCACAG 
CACCTGGGCT GTACAATCAA GCTTAGAGGA GGCTCCGATT ATGAGCTGGC TCGAGTTAAG 
GAGATCCTCA TATTTATGAT CTGTGTAGCA TATCATTCTC AACTAGAAAT CTCCTTCCTC 
ATGGATGAAT TTGCCATGCC TCCTACATTA ACGCAAAACC CTTCCTTCCA CTCTCTGATT 
GAGGAACAGG AGGATGACGA GGCTGCTCAA GAACAGCTCA GCGGAAGTCC CCTCCCCCGG 
GAACCTGACT TCCCTCTGGA GTGTCTGGCC CCTGATGAGA GCAGTTCACT GGAATCAAGG 
ACTGTGTTTG GGAAGAGTGA CCAGGAACTT AAAGGTGCTC CAAAGGATGC TGTCTCGTTG 
AAGCAGCAGG AGTTCGCCAC AGCAGCCTGC CCGGCCGGCG TCCCCTATGC TCTCTTTGCA 
TTGGTCCCAG AATCCTTGTT GCCACTGCAT CTGGGTGACC AACAGGATGC CAGGGGCAGC 
GAGCAGCCAG AGACTGTGCA GCAAACGGGT GAGCTACAGG ATCCCAAAAG CCAGATGAGA 
GCCTTTAGAG ACCCTCTGCA GGATGACACT GGATTGTATG TCACTGAGGA AGTCACCTCT 
TCTGAGGATA AGCGGAAGAC TTACTCTTTG GCCTTTAAAC AGGAATTAAA AGACGTAATT 
CTCTGTATCT CCCCAGTGAT CACATTCCGA GAGCCTTTCC TTTTAACCGA GAAGGGGATG 
CGATGCTCTC CCCGAGACTA TTTCGCAGAG CAGCTTTACT GGTCTCCTCT CCTCAATAAA 
GACTTCAAAG AAATGGAGAG CAGGCGGAAG AAGCAGCTGC TCAGGGATCT CTCCAGCCTC 
CAGGGCCTGA ACGGAAGTGT GCAGGCCAAG TCTATCCAAG TGCTGCCCTC ACACGAGCTG 
GTGAGCGCTA GGATTGCTGA GCATCTGGGT GACAGCCAGG GCCTGGGCCG GATGCTGGCT 
GACTACCGGG CCAGAGGAGG GAGAATTCAG CAGAATACTT CAGACCCTTT TGCTCATTCA 
AAGGATGCAT CAGGTCCTTC AAGTGGCAAG TCAGGAAGCA GAACTGAGGG CGATGAAGAG 
AAAGGGCTGA TTCCGAGTGA CGCGGTATGG TCAACAAAGG TGGACTGTCT GAACCCTGCC 
AACCACCAGA GGCTGTGCGT GCTCTTCAGC AGCTCTTCTG CCCAGTCCAG CAACGCGCCC 
AGCGCCTGCG TCAGTCCTTG GATTGTAACA ATGGAATTTT ATGGAAAGAA TGATCTTACA 
CTGGGAATAT TTTTAGAGAG ATACTGTTTC AGGCCTTCTT ACCAGTGTCC TAGCATGTTC 
TGTGATACCC CCATGGTACA TCACATTCGG CGCTTTGTCC ATGGCCAAGG CTGTGTGCAG 
ATAATCCTGA AGGAGCTGGA TTCTCCAGTA CCTGGATATC AGCATACAAT TCTCACCTAT 
TCCTGGTGCA GAATCTGCAA ACAGGTAACA CCAGTTGTTG CTCTTTCCAA TGAGTCCTGG 
TCCATGTCAT TTGCCAAATA CCTTGAACTT CGGTTTTATG GGCACCAGTA CACCCGTAGA 
GCCAATGCTG AGCCGTGTGG CCACTCCATC CACCATGATT ATCACCAGTA CTTCTCCTAT 
AACCAGATGG TGGCATCTTT TAGTTATTCT CCCATTCGGC TTCTTGAAGT ATGTGTTCCA 
CTCCCCAAAA TATTCATTAA ACGTCAGGCC CCATTAAAAG TGTCCCTTCT TCAAGATCTG 
AAGGACTTCT TTCAAAAAGT TTCACAAGTA TATCTTGCTG TTGATGAAAG ACTTGCATCT 
TTGAAAACTG ATACATTTAG CAAAACAAGA GAGGAAAAAA TGGAAGATAT CTTTGCACAA 
AAAGAGATGG AGGAAGGCGA GTTCAAGAAC TGGATTGAGA AAATGCAAGC GAGGCTCCTG 
TCTTCCTCGG TAGACACCCC TCAGCAGCTG CAGTCGGTCC TGGAGTCGCT CATCGCCAAG 
AAGCAGAGCC TCTGTGAAGT GCTGCAGGCC TGGAATAACA GGTTGCAGGA CCTTTTCCAG 
CAGGAAAAGG GTAGAAAGCG GCCTTCAGTT CCTCCAAGTC CTGGAAGACT GAGACAAGGG 
GAAGAAAGCA AGATAAGTGC AATGGATGCA TCTCCACGGA ATATTTCTCC AGGACTTCAA 
AATGGAGAAA AAGAGGACCG CTTCTTGACA ACCCTCTCTA GTCAGGGCCC CACCTCTGCT 
GCTCATCTCC AGCTGCCCAG TCCCCCTGAA GTCCTGCCTG AGCAGGCAGG GGGGGCGACC 
CCCGAGCCGG ATGCAGCCAG CAGTTCTGAA GATGTGTTTG ATGGACATTC TCTGGGATCC 
ACAGACAGCC AGGTGAAGGA AAAATCAACC ATGAAGGCCA TCTTTGCAAA TTTGCTTCCA 
GGAAATAGCT ATAACCCTAT TCCGTTTCCT TTTGATCCAG ATAAACACTA CTTAATGTAC 
GAACACGAAC GGGTGCCCAT TGCCGTCTGT GAGAGGGAGC CCAGCTCCAT CATTGCTTTT 
GCTCTCAGTT GTAAAGAATA CCGAAATGCC TTAGAGGAAT TGTCCAAGGC AACTCTGCGG 
AACAGTGCCG AAGAAGGGCT CCCAGCCAAC AGTGCTTCAG ACAGCAGACC AAAGGGCGGC 
AGCCCTGGGA GGCCGCCTGA GCTCGGTGGA GGCTGCCCCA ACCGGGCGGC CGAGGCAGAG 
CCGCAGCCAG CCAAAAAGGC TTCTGGGATG TTGTCCTTCT TCCGAGGGAC AGCAGGGAAA 
AGCCCTGATC TCTCTTCCCA GAAGAGAGAG ACCTTACGTG GAGCAGATAG TGCTTACTAC 
CAGGTCGGGC AGCCCGGCAA GGAGGGGACG GAGAGTCAAG GCACTGAGCC TCCAGATGAG 
ATAGATGGAG GAGACGCACA AAAGAAACAG CTCACAAATC CTCATGTGGA ACTTCAATTT 
TCTGACGCTA ACGCCAAGTT TTACTGCCGG CTCTACTATG CGGGAGAGTT TCACAAGATG 
CGCGAGGTGA TCCTGGGCAG CAGCGAGGAG GACTTCATCC GCTCCCTTTC CCACTCGGCA 
CCCTGGCAGG CACGCGGGGG CAAGTCCGGA GCGGCCTTCT ACGCCACGGA AGATGATAGA 
TTCATTTTGA AGCAGATGCC TCGTCTGGAA GTCCAGTCTT TCCTCGACTT TGCACCACAC 
TACTTCAATT ATATCACAAA CGCTGTGCAA CAAAAGAGGC CTACTGCACT GGCCAAAATT 
CTTGGAGTTT ACAGAATTGG TTATAAGAAT TCTCAGAACA ACACTGAGAA GAAGTTAGAT 
CTCCTTGTCA TGGAAAATCT TTTCTATGGG AGAAAGATGG CACAGGTTTT TGACTTGAAG 
GGTTCACTTA GGAACAGAAA TGTAAAAACT GACACCGGGA AAGAGAGCTG TGACGTGGTC 
CTGCTGGACG AGAATCTGCT GAAGATGGTC CGGGACAGCC CTCTGTACAT CCGCTCGCAC 
TCCAAGGCGG TGCTGCGGGC CTCCATCCGC AGCGATGCCC ACTTCCTGTC CAGCCACCTC 
ATCATCGACT ATTCTTTGCT GGTGGGGCGA GATGATACCA GCAATGAGCT TGTCGTTGGG 
ATTATAGATT ATATTCGAAC GTTTACATGG GACAAAAAGC TTGAGATGGT TGTGAAATCA 
ACAGGAATTT TAGGAGGACA AGGTAAAATG CCAACTGTGG TCTCTCCAGA GTTGTACAGG 
ACCCGATTTT GTGAAGCGAT GGACAAGTAT TTCCTCATGG TACCAGACCA CTGGACAGGC 
TTGGGTCTCA ACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023478290.1
CDS	114..6491
Translation	MATDDKTSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPAFKSAYSSFVNLFRFNKDRAEGEQQSLTGGWPSPQLPSRTQSVRSPIPYKKQLTEELQRRPSAVLAENSLQHPQENTDTRRKTEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCRECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRMDLAVPAPLDAVWIKSYIHGPTGSASITNLSLDRSGSPLVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAVAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTDFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEDSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSPSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHVKKIPGGKKFDSMVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDLLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPGEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEEQEDDEAAQEQLSGSPLPREPDFPLECLAPDESSSLESRTVFGKSDQELKGAPKDAVSLKQQEFATAACPAGVPYALFALVPESLLPLHLGDQQDARGSEQPETVQQTGELQDPKSQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSPRDYFAEQLYWSPLLNKDFKEMESRRKKQLLRDLSSLQGLNGSVQAKSIQVLPSHELVSARIAEHLGDSQGLGRMLADYRARGGRIQQNTSDPFAHSKDASGPSSGKSGSRTEGDEEKGLIPSDAVWSTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLLSSSVDTPQQLQSVLESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQGPTSAAHLQLPSPPEVLPEQAGGATPEPDAASSSEDVFDGHSLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSASDSRPKGGSPGRPPELGGGCPNRAAEAEPQPAKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQPGKEGTESQGTEPPDEIDGGDAQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSAPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDSPLYIRSHSKAVLRASIRSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_023622522.1
Organism	Equus caballus(horse)
Definition	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023622522.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGCAGA GAACAGTTTG CAGCACCCCC AGGAGAACAC AGACACAAGA 
AGGAAAACAG AACCTACCTT TGGCGGTCAT GACCCTCGTA CAGCTGTTCA GCTTCGAAGC 
CTCAGCACAG TGTTAAAACG CCTCAAGGAA ATCATGGAGG GGAAAAGCCA GGACAGTGAC 
CTGAAGCAGT ACTGGATGCC GGACAGCCAG TGCAGAGAGT GCTACGACTG CAGCGAGAAG 
TTCACCACCT TCCGGCGCAG GCACCACTGC CGGCTCTGCG GGCAGATTTT CTGCAGCCGC 
TGCTGCAATC AAGAGATCCC TGGGAAGTTT ATGGGCTATA CAGGGGACCT CCGAGCTTGC 
ACATACTGTA GAAAAATAGC CTTAAGTTAT GCTCATTCCA CAGACAGTAA TTCCATTGGG 
GAAGACTTGA ATGCTCTTTC AGATTCTGCT TCCTCTGTGT CTGTACTTGA TCCGAGTGAA 
CCCCGAACAC CTGTTGGGAG TAGGAAAGCC AGCCGGAACA TCTTTTTAGA GGATGATTTT 
GCCTGGCAAA GTTTGATTCA TCCAGACTCC TCAAATACTG CTCTTTCAAC AAGACTTGTA 
TCTGTTCAAG AGGACGCTGG GAAATCTCCT GCTCGAAATA GAATGGACTT GGCAGTGCCT 
GCTCCATTGG ATGCTGTGTG GATTAAATCA TACATACATG GCCCTACTGG GTCAGCCAGC 
ATTACCAACC TCTCACTGGA TCGATCTGGT TCTCCCCTGG TACCTTCATA TGAGACATCT 
GTCAGTCCCC AGGCCAACCG AACATACGTG AGGACAGAGA CCACAGAGGA TGAACGCAAA 
ATTCTTCTGG ACAGCGTCCA GTTAAAAGAC CTGTGGAAGA AAATCTGCCA TCACAGTAGT 
GGAATGGAGT TTCAGGATCA CCGCTACTGG CTGAGGACAC ATCCCAACTG CATCGTAGGG 
AAGGAGTTAG TCAACTGGCT AATCCGAAAT GGGCACATTG CCACAAGGGC ACAAGCTGTA 
GCAATTGGAC AAGCGATGGT GGACGGACGG TGGCTGGACT GCGTGAGCCA CCATGACCAG 
CTCTTCAGGG ATGAGTATGC GCTCTACAGA CCCCTGCAGA GTACAGACTT TTCTGAGACC 
CCTTCTCCGG ACAGTGACTC CGTGAACTCT GTGGAAGGAC ACTCGGAGCC ATCATGGTTT 
AAAGACATAA AGTTTGATGA CAGCGACACA GAGCAGATTG CTGAAGAAGA CTCTGCCAGT 
CCTAGCAAGC GCACGTCAGT CAGCAGTTTC CAGTCCACAG TGGACAGTGA CTCAGCCGCC 
TCCATCAGCC TGAACGTGGA GTTGGACAAC GTGAACTTCC ACATCAAGAA GCCCTCCAAG 
TACCCACACG TGCCCCCTCA CCCTGCTGAC CAAAAAGAGT ATTTGATTTC TGACAACGGA 
GGACAACAAC TCTCAATAAG TGACGCCTTC ATCAAAGAAT CCTTATTTAA TCGTCGAGTT 
GAGGAGAAAT CCAAAGAACT GCCCTTTACG CCTTTGGGCT GGCATCATAA CAATCTGGAG 
CTGCTGCGGG AGGAGAATGG AGAGAAGCAG GCCATGGAGA GGTTGCTTTC AGCTAATCAT 
AACCACATGA TGGCACTGCT CCAACAGTTG CTCCATAGTG AGTCATTGTC ACCATCTTGG 
AGGGACATCA TTGTATCCCT GGTCTGCCAG GTTGTTCAGA CAGTCCGACC TGACGTCAAG 
AACCGGGATG ACGACATGGA TATCCGTCAG TTTGTCCACG TCAAAAAAAT CCCAGGTGGA 
AAGAAGTTTG ATTCTATGGT TGTCAATGGG TTCGTTTGTA CCAAGAATAT TGCACATAAA 
AAGATGAATT CTTGTATTAA AAACCCCAAA ATTCTTCTGT TGAAGTGTTC CATTGAGTAT 
CTCTACAGAG AAGAAACTAA ATTTACTTGC ATTGACCCTA TCGTGCTTCA GGAAAGGGAA 
TTCTTGAAGA ATTATGTTCA GCGAATAGTG GATGTTCGAC CCACATTGGT TCTAGTTGAG 
AAAACGGTGT CTCGGATTGC CCAGGACCTG TTGCTGGAAC ACGGCATCAC TTTGGTCATT 
AATGTCAAAT CACAAGTTTT AGAACGAATC AGTCGGATGA CCCAAGGTGA TTTAGTGATG 
TCGATGGACC AGTTGCTCAC AAAGCCCCAC CTGGGCACCT GTCACAAATT TTACATGCAG 
ATGTTTCAGC TACCTGGTGA ACAAACCAAA ACTCTGATGT TTTTTGAAGG CTGTCCACAG 
CACCTGGGCT GTACAATCAA GCTTAGAGGA GGCTCCGATT ATGAGCTGGC TCGAGTTAAG 
GAGATCCTCA TATTTATGAT CTGTGTAGCA TATCATTCTC AACTAGAAAT CTCCTTCCTC 
ATGGATGAAT TTGCCATGCC TCCTACATTA ACGCAAAACC CTTCCTTCCA CTCTCTGATT 
GAGGAACAGG AGGATGACGA GGCTGCTCAA GAACAGCTCA GCGGAAGTCC CCTCCCCCGG 
GAACCTGACT TCCCTCTGGA GTGTCTGGCC CCTGATGAGA GCAGTTCACT GGAATCAAGG 
ACTGTGTTTG GGAAGAGTGA CCAGGAACTT AAAGGTGCTC CAAAGGATGC TGTCTCGTTG 
AAGCAGCAGG AGTTCGCCAC AGCAGCCTGC CCGGCCGGCG TCCCCTATGC TCTCTTTGCA 
TTGGTCCCAG AATCCTTGTT GCCACTGCAT CTGGGTGACC AACAGGATGC CAGGGGCAGC 
GAGCAGCCAG AGACTGTGCA GCAAACGGGT GAGCTACAGG ATCCCAAAAG CCAGATGAGA 
GCCTTTAGAG ACCCTCTGCA GGATGACACT GGATTGTATG TCACTGAGGA AGTCACCTCT 
TCTGAGGATA AGCGGAAGAC TTACTCTTTG GCCTTTAAAC AGGAATTAAA AGACGTAATT 
CTCTGTATCT CCCCAGTGAT CACATTCCGA GAGCCTTTCC TTTTAACCGA GAAGGGGATG 
CGATGCTCTC CCCGAGACTA TTTCGCAGAG CAGCTTTACT GGTCTCCTCT CCTCAATAAA 
GACTTCAAAG AAATGGAGAG CAGGCGGAAG AAGCAGCTGC TCAGGGATCT CTCCAGCCTC 
CAGGGCCTGA ACGGAAGTGT GCAGGCCAAG TCTATCCAAG TGCTGCCCTC ACACGAGCTG 
GTGAGCGCTA GGATTGCTGA GCATCTGGGT GACAGCCAGG GCCTGGGCCG GATGCTGGCT 
GACTACCGGG CCAGAGGAGG GAGAATTCAG CAGAATACTT CAGACCCTTT TGCTCATTCA 
AAGGATGCAT CAGGTCCTTC AAGTGGCAAG TCAGGAAGCA GAACTGAGGG CGATGAAGAG 
AAAGGGCTGA TTCCGAGTGA CGCGGTATGG TCAACAAAGG TGGACTGTCT GAACCCTGCC 
AACCACCAGA GGCTGTGCGT GCTCTTCAGC AGCTCTTCTG CCCAGTCCAG CAACGCGCCC 
AGCGCCTGCG TCAGTCCTTG GATTGTAACA ATGGAATTTT ATGGAAAGAA TGATCTTACA 
CTGGGAATAT TTTTAGAGAG ATACTGTTTC AGGCCTTCTT ACCAGTGTCC TAGCATGTTC 
TGTGATACCC CCATGGTACA TCACATTCGG CGCTTTGTCC ATGGCCAAGG CTGTGTGCAG 
ATAATCCTGA AGGAGCTGGA TTCTCCAGTA CCTGGATATC AGCATACAAT TCTCACCTAT 
TCCTGGTGCA GAATCTGCAA ACAGGTAACA CCAGTTGTTG CTCTTTCCAA TGAGTCCTGG 
TCCATGTCAT TTGCCAAATA CCTTGAACTT CGGTTTTATG GGCACCAGTA CACCCGTAGA 
GCCAATGCTG AGCCGTGTGG CCACTCCATC CACCATGATT ATCACCAGTA CTTCTCCTAT 
AACCAGATGG TGGCATCTTT TAGTTATTCT CCCATTCGGC TTCTTGAAGT ATGTGTTCCA 
CTCCCCAAAA TATTCATTAA ACGTCAGGCC CCATTAAAAG TGTCCCTTCT TCAAGATCTG 
AAGGACTTCT TTCAAAAAGT TTCACAAGTA TATCTTGCTG TTGATGAAAG ACTTGCATCT 
TTGAAAACTG ATACATTTAG CAAAACAAGA GAGGAAAAAA TGGAAGATAT CTTTGCACAA 
AAAGAGATGG AGGAAGGCGA GTTCAAGAAC TGGATTGAGA AAATGCAAGC GAGGCTCCTG 
TCTTCCTCGG TAGACACCCC TCAGCAGCTG CAGTCGGTCC TGGAGTCGCT CATCGCCAAG 
AAGCAGAGCC TCTGTGAAGT GCTGCAGGCC TGGAATAACA GGTTGCAGGA CCTTTTCCAG 
CAGGAAAAGG GTAGAAAGCG GCCTTCAGTT CCTCCAAGTC CTGGAAGACT GAGACAAGGG 
GAAGAAAGCA AGATAAGTGC AATGGATGCA TCTCCACGGA ATATTTCTCC AGGACTTCAA 
AATGGAGAAA AAGAGGACCG CTTCTTGACA ACCCTCTCTA GTCAGGGCCC CACCTCTGCT 
GCTCATCTCC AGCTGCCCAG TCCCCCTGAA GTCCTGCCTG AGCAGGCAGG GGGGGCGACC 
CCCGAGCCGG ATGCAGCCAG CAGTTCTGAA GATGTGTTTG ATGGACATTC TCTGGGATCC 
ACAGACAGCC AGGTGAAGGA AAAATCAACC ATGAAGGCCA TCTTTGCAAA TTTGCTTCCA 
GGAAATAGCT ATAACCCTAT TCCGTTTCCT TTTGATCCAG ATAAACACTA CTTAATGTAC 
GAACACGAAC GGGTGCCCAT TGCCGTCTGT GAGAGGGAGC CCAGCTCCAT CATTGCTTTT 
GCTCTCAGTT GTAAAGAATA CCGAAATGCC TTAGAGGAAT TGTCCAAGGC AACTCTGCGG 
AACAGTGCCG AAGAAGGGCT CCCAGCCAAC AGTGCTTCAG ACAGCAGACC AAAGGGCGGC 
AGCCCTGGGA GGCCGCCTGA GCTCGGTGGA GGCTGCCCCA ACCGGGCGGC CGAGGCAGAG 
CCGCAGCCAG CCAAAAAGGC TTCTGGGATG TTGTCCTTCT TCCGAGGGAC AGCAGGGAAA 
AGCCCTGATC TCTCTTCCCA GAAGAGAGAG ACCTTACGTG GAGCAGATAG TGCTTACTAC 
CAGGTCGGGC AGCCCGGCAA GGAGGGGACG GAGAGTCAAG GCACTGAGCC TCCAGATGAG 
ATAGATGGAG GAGACGCACA AAAGAAACAG CTCACAAATC CTCATGTGGA ACTTCAATTT 
TCTGACGCTA ACGCCAAGTT TTACTGCCGG CTCTACTATG CGGGAGAGTT TCACAAGATG 
CGCGAGGTGA TCCTGGGCAG CAGCGAGGAG GACTTCATCC GCTCCCTTTC CCACTCGGCA 
CCCTGGCAGG CACGCGGGGG CAAGTCCGGA GCGGCCTTCT ACGCCACGGA AGATGATAGA 
TTCATTTTGA AGCAGATGCC TCGTCTGGAA GTCCAGTCTT TCCTCGACTT TGCACCACAC 
TACTTCAATT ATATCACAAA CGCTGTGCAA CAAAAGAGGC CTACTGCACT GGCCAAAATT 
CTTGGAGTTT ACAGAATTGG TTATAAGAAT TCTCAGAACA ACACTGAGAA GAAGTTAGAT 
CTCCTTGTCA TGGAAAATCT TTTCTATGGG AGAAAGATGG CACAGGTTTT TGACTTGAAG 
GGTTCACTTA GGAACAGAAA TGTAAAAACT GACACCGGGA AAGAGAGCTG TGACGTGGTC 
CTGCTGGACG AGAATCTGCT GAAGATGGTC CGGGACAGCC CTCTGTACAT CCGCTCGCAC 
TCCAAGGCGG TGCTGCGGGC CTCCATCCGC AGCGATGCCC ACTTCCTGTC CAGCCACCTC 
ATCATCGACT ATTCTTTGCT GGTGGGGCGA GATGATACCA GCAATGAGCT TGTCGTTGGG 
ATTATAGATT ATATTCGAAC GTTTACATGG GACAAAAAGC TTGAGATGGT TGTGAAATCA 
ACAGGAATTT TAGGAGGACA AGGTAAAATG CCAACTGTGG TCTCTCCAGA GTTGTACAGG 
ACCCGATTTT GTGAAGCGAT GGACAAGTAT TTCCTCATGG TACCAGACCA CTGGACAGGC 
TTGGGTCTCA ACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_023622523.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023478291.1 Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X7, mRNA.

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CloneID	OHb52472
Clone ID Related Accession (Same CDS sequence)	XM_023622523.1
Accession Version	XM_023622523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGACAC AAGAAGGAAA ACAGAACCTA CCTTTGGCGG TCATGACCCT 
CGTACAGCTG TTCAGCTTCG AAGCCTCAGC ACAGTGTTAA AACGCCTCAA GGAAATCATG 
GAGGGGAAAA GCCAGGACAG TGACCTGAAG CAGTACTGGA TGCCGGACAG CCAGTGCAGA 
GAGTGCTACG ACTGCAGCGA GAAGTTCACC ACCTTCCGGC GCAGGCACCA CTGCCGGCTC 
TGCGGGCAGA TTTTCTGCAG CCGCTGCTGC AATCAAGAGA TCCCTGGGAA GTTTATGGGC 
TATACAGGGG ACCTCCGAGC TTGCACATAC TGTAGAAAAA TAGCCTTAAG TTATGCTCAT 
TCCACAGACA GTAATTCCAT TGGGGAAGAC TTGAATGCTC TTTCAGATTC TGCTTCCTCT 
GTGTCTGTAC TTGATCCGAG TGAACCCCGA ACACCTGTTG GGAGTAGGAA AGCCAGCCGG 
AACATCTTTT TAGAGGATGA TTTTGCCTGG CAAAGTTTGA TTCATCCAGA CTCCTCAAAT 
ACTGCTCTTT CAACAAGACT TGTATCTGTT CAAGAGGACG CTGGGAAATC TCCTGCTCGA 
AATAGAATGG ACTTGGCAGT GCCTGCTCCA TTGGATGCTG TGTGGATTAA ATCATACATA 
CATGGCCCTA CTGGGTCAGC CAGCATTACC AACCTCTCAC TGGATCGATC TGGTTCTCCC 
CTGGTACCTT CATATGAGAC ATCTGTCAGT CCCCAGGCCA ACCGAACATA CGTGAGGACA 
GAGACCACAG AGGATGAACG CAAAATTCTT CTGGACAGCG TCCAGTTAAA AGACCTGTGG 
AAGAAAATCT GCCATCACAG TAGTGGAATG GAGTTTCAGG ATCACCGCTA CTGGCTGAGG 
ACACATCCCA ACTGCATCGT AGGGAAGGAG TTAGTCAACT GGCTAATCCG AAATGGGCAC 
ATTGCCACAA GGGCACAAGC TGTAGCAATT GGACAAGCGA TGGTGGACGG ACGGTGGCTG 
GACTGCGTGA GCCACCATGA CCAGCTCTTC AGGGATGAGT ATGCGCTCTA CAGACCCCTG 
CAGAGTACAG ACTTTTCTGA GACCCCTTCT CCGGACAGTG ACTCCGTGAA CTCTGTGGAA 
GGACACTCGG AGCCATCATG GTTTAAAGAC ATAAAGTTTG ATGACAGCGA CACAGAGCAG 
ATTGCTGAAG AAGGTGACGA TAATTTGACT AACTCTGCCA GTCCTAGCAA GCGCACGTCA 
GTCAGCAGTT TCCAGTCCAC AGTGGACAGT GACTCAGCCG CCTCCATCAG CCTGAACGTG 
GAGTTGGACA ACGTGAACTT CCACATCAAG AAGCCCTCCA AGTACCCACA CGTGCCCCCT 
CACCCTGCTG ACCAAAAAGA GTATTTGATT TCTGACAACG GAGGACAACA ACTCTCAATA 
AGTGACGCCT TCATCAAAGA ATCCTTATTT AATCGTCGAG TTGAGGAGAA ATCCAAAGAA 
CTGCCCTTTA CGCCTTTGGG CTGGCATCAT AACAATCTGG AGCTGCTGCG GGAGGAGAAT 
GGAGAGAAGC AGGCCATGGA GAGGTTGCTT TCAGCTAATC ATAACCACAT GATGGCACTG 
CTCCAACAGT TGCTCCATAG TGAGTCATTG TCACCATCTT GGAGGGACAT CATTGTATCC 
CTGGTCTGCC AGGTTGTTCA GACAGTCCGA CCTGACGTCA AGAACCGGGA TGACGACATG 
GATATCCGTC AGTTTGTCCA CGTCAAAAAA ATCCCAGGTG GAAAGAAGTT TGATTCTATG 
GTTGTCAATG GGTTCGTTTG TACCAAGAAT ATTGCACATA AAAAGATGAA TTCTTGTATT 
AAAAACCCCA AAATTCTTCT GTTGAAGTGT TCCATTGAGT ATCTCTACAG AGAAGAAACT 
AAATTTACTT GCATTGACCC TATCGTGCTT CAGGAAAGGG AATTCTTGAA GAATTATGTT 
CAGCGAATAG TGGATGTTCG ACCCACATTG GTTCTAGTTG AGAAAACGGT GTCTCGGATT 
GCCCAGGACC TGTTGCTGGA ACACGGCATC ACTTTGGTCA TTAATGTCAA ATCACAAGTT 
TTAGAACGAA TCAGTCGGAT GACCCAAGGT GATTTAGTGA TGTCGATGGA CCAGTTGCTC 
ACAAAGCCCC ACCTGGGCAC CTGTCACAAA TTTTACATGC AGATGTTTCA GCTACCTGGT 
GAACAAACCA AAACTCTGAT GTTTTTTGAA GGCTGTCCAC AGCACCTGGG CTGTACAATC 
AAGCTTAGAG GAGGCTCCGA TTATGAGCTG GCTCGAGTTA AGGAGATCCT CATATTTATG 
ATCTGTGTAG CATATCATTC TCAACTAGAA ATCTCCTTCC TCATGGATGA ATTTGCCATG 
CCTCCTACAT TAACGCAAAA CCCTTCCTTC CACTCTCTGA TTGAGGAACA GGAGGATGAC 
GAGGCTGCTC AAGAACAGCT CAGCGGAAGT CCCCTCCCCC GGGAACCTGA CTTCCCTCTG 
GAGTGTCTGG CCCCTGATGA GAGCAGTTCA CTGGAATCAA GGACTGTGTT TGGGAAGAGT 
GACCAGGAAC TTAAAGGTGC TCCAAAGGAT GCTGTCTCGT TGAAGCAGCA GGAGTTCGCC 
ACAGCAGCCT GCCCGGCCGG CGTCCCCTAT GCTCTCTTTG CATTGGTCCC AGAATCCTTG 
TTGCCACTGC ATCTGGGTGA CCAACAGGAT GCCAGGGGCA GCGAGCAGCC AGAGACTGTG 
CAGCAAACGG GTGAGCTACA GGATCCCAAA AGCCAGATGA GAGCCTTTAG AGACCCTCTG 
CAGGATGACA CTGGATTGTA TGTCACTGAG GAAGTCACCT CTTCTGAGGA TAAGCGGAAG 
ACTTACTCTT TGGCCTTTAA ACAGGAATTA AAAGACGTAA TTCTCTGTAT CTCCCCAGTG 
ATCACATTCC GAGAGCCTTT CCTTTTAACC GAGAAGGGGA TGCGATGCTC TCCCCGAGAC 
TATTTCGCAG AGCAGCTTTA CTGGTCTCCT CTCCTCAATA AAGACTTCAA AGAAATGGAG 
AGCAGGCGGA AGAAGCAGCT GCTCAGGGAT CTCTCCAGCC TCCAGGGCCT GAACGGAAGT 
GTGCAGGCCA AGTCTATCCA AGTGCTGCCC TCACACGAGC TGGTGAGCGC TAGGATTGCT 
GAGCATCTGG GTGACAGCCA GGGCCTGGGC CGGATGCTGG CTGACTACCG GGCCAGAGGA 
GGGAGAATTC AGCAGAATAC TTCAGACCCT TTTGCTCATT CAAAGGATGC ATCAGGTCCT 
TCAAGTGGCA AGTCAGGAAG CAGAACTGAG GGCGATGAAG AGAAAGGGCT GATTCCGAGT 
GACGCGGTAT GGTCAACAAA GGTGGACTGT CTGAACCCTG CCAACCACCA GAGGCTGTGC 
GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC AGCAACGCGC CCAGCGCCTG CGTCAGTCCT 
TGGATTGTAA CAATGGAATT TTATGGAAAG AATGATCTTA CACTGGGAAT ATTTTTAGAG 
AGATACTGTT TCAGGCCTTC TTACCAGTGT CCTAGCATGT TCTGTGATAC CCCCATGGTA 
CATCACATTC GGCGCTTTGT CCATGGCCAA GGCTGTGTGC AGATAATCCT GAAGGAGCTG 
GATTCTCCAG TACCTGGATA TCAGCATACA ATTCTCACCT ATTCCTGGTG CAGAATCTGC 
AAACAGGTAA CACCAGTTGT TGCTCTTTCC AATGAGTCCT GGTCCATGTC ATTTGCCAAA 
TACCTTGAAC TTCGGTTTTA TGGGCACCAG TACACCCGTA GAGCCAATGC TGAGCCGTGT 
GGCCACTCCA TCCACCATGA TTATCACCAG TACTTCTCCT ATAACCAGAT GGTGGCATCT 
TTTAGTTATT CTCCCATTCG GCTTCTTGAA GTATGTGTTC CACTCCCCAA AATATTCATT 
AAACGTCAGG CCCCATTAAA AGTGTCCCTT CTTCAAGATC TGAAGGACTT CTTTCAAAAA 
GTTTCACAAG TATATCTTGC TGTTGATGAA AGACTTGCAT CTTTGAAAAC TGATACATTT 
AGCAAAACAA GAGAGGAAAA AATGGAAGAT ATCTTTGCAC AAAAAGAGAT GGAGGAAGGC 
GAGTTCAAGA ACTGGATTGA GAAAATGCAA GCGAGGCTCC TGTCTTCCTC GGTAGACACC 
CCTCAGCAGC TGCAGTCGGT CCTGGAGTCG CTCATCGCCA AGAAGCAGAG CCTCTGTGAA 
GTGCTGCAGG CCTGGAATAA CAGGTTGCAG GACCTTTTCC AGCAGGAAAA GGGTAGAAAG 
CGGCCTTCAG TTCCTCCAAG TCCTGGAAGA CTGAGACAAG GGGAAGAAAG CAAGATAAGT 
GCAATGGATG CATCTCCACG GAATATTTCT CCAGGACTTC AAAATGGAGA AAAAGAGGAC 
CGCTTCTTGA CAACCCTCTC TAGTCAGGGC CCCACCTCTG CTGCTCATCT CCAGCTGCCC 
AGTCCCCCTG AAGTCCTGCC TGAGCAGGCA GGGGGGGCGA CCCCCGAGCC GGATGCAGCC 
AGCAGTTCTG AAGATGTGTT TGATGGACAT TCTCTGGGAT CCACAGACAG CCAGGTGAAG 
GAAAAATCAA CCATGAAGGC CATCTTTGCA AATTTGCTTC CAGGAAATAG CTATAACCCT 
ATTCCGTTTC CTTTTGATCC AGATAAACAC TACTTAATGT ACGAACACGA ACGGGTGCCC 
ATTGCCGTCT GTGAGAGGGA GCCCAGCTCC ATCATTGCTT TTGCTCTCAG TTGTAAAGAA 
TACCGAAATG CCTTAGAGGA ATTGTCCAAG GCAACTCTGC GGAACAGTGC CGAAGAAGGG 
CTCCCAGCCA ACAGTGCTTC AGACAGCAGA CCAAAGGGCG GCAGCCCTGG GAGGCCGCCT 
GAGCTCGGTG GAGGCTGCCC CAACCGGGCG GCCGAGGCAG AGCCGCAGCC AGCCAAAAAG 
GCTTCTGGGA TGTTGTCCTT CTTCCGAGGG ACAGCAGGGA AAAGCCCTGA TCTCTCTTCC 
CAGAAGAGAG AGACCTTACG TGGAGCAGAT AGTGCTTACT ACCAGGTCGG GCAGCCCGGC 
AAGGAGGGGA CGGAGAGTCA AGGCACTGAG CCTCCAGATG AGATAGATGG AGGAGACGCA 
CAAAAGAAAC AGCTCACAAA TCCTCATGTG GAACTTCAAT TTTCTGACGC TAACGCCAAG 
TTTTACTGCC GGCTCTACTA TGCGGGAGAG TTTCACAAGA TGCGCGAGGT GATCCTGGGC 
AGCAGCGAGG AGGACTTCAT CCGCTCCCTT TCCCACTCGG CACCCTGGCA GGCACGCGGG 
GGCAAGTCCG GAGCGGCCTT CTACGCCACG GAAGATGATA GATTCATTTT GAAGCAGATG 
CCTCGTCTGG AAGTCCAGTC TTTCCTCGAC TTTGCACCAC ACTACTTCAA TTATATCACA 
AACGCTGTGC AACAAAAGAG GCCTACTGCA CTGGCCAAAA TTCTTGGAGT TTACAGAATT 
GGTTATAAGA ATTCTCAGAA CAACACTGAG AAGAAGTTAG ATCTCCTTGT CATGGAAAAT 
CTTTTCTATG GGAGAAAGAT GGCACAGGTT TTTGACTTGA AGGGTTCACT TAGGAACAGA 
AATGTAAAAA CTGACACCGG GAAAGAGAGC TGTGACGTGG TCCTGCTGGA CGAGAATCTG 
CTGAAGATGG TCCGGGACAG CCCTCTGTAC ATCCGCTCGC ACTCCAAGGC GGTGCTGCGG 
GCCTCCATCC GCAGCGATGC CCACTTCCTG TCCAGCCACC TCATCATCGA CTATTCTTTG 
CTGGTGGGGC GAGATGATAC CAGCAATGAG CTTGTCGTTG GGATTATAGA TTATATTCGA 
ACGTTTACAT GGGACAAAAA GCTTGAGATG GTTGTGAAAT CAACAGGAAT TTTAGGAGGA 
CAAGGTAAAA TGCCAACTGT GGTCTCTCCA GAGTTGTACA GGACCCGATT TTGTGAAGCG 
ATGGACAAGT ATTTCCTCAT GGTACCAGAC CACTGGACAG GCTTGGGTCT CAACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023478291.1
CDS	114..6473
Translation	MATDDKTSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPAFKSAYSSFVNLFRFNKDRAEGEQQSLTGGWPSPQLPSRTQSVRSPIPYKKQLTEELQRRPSAVLDTRRKTEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCRECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRMDLAVPAPLDAVWIKSYIHGPTGSASITNLSLDRSGSPLVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAVAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTDFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSPSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHVKKIPGGKKFDSMVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDLLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPGEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEEQEDDEAAQEQLSGSPLPREPDFPLECLAPDESSSLESRTVFGKSDQELKGAPKDAVSLKQQEFATAACPAGVPYALFALVPESLLPLHLGDQQDARGSEQPETVQQTGELQDPKSQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSPRDYFAEQLYWSPLLNKDFKEMESRRKKQLLRDLSSLQGLNGSVQAKSIQVLPSHELVSARIAEHLGDSQGLGRMLADYRARGGRIQQNTSDPFAHSKDASGPSSGKSGSRTEGDEEKGLIPSDAVWSTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLLSSSVDTPQQLQSVLESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQGPTSAAHLQLPSPPEVLPEQAGGATPEPDAASSSEDVFDGHSLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSASDSRPKGGSPGRPPELGGGCPNRAAEAEPQPAKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQPGKEGTESQGTEPPDEIDGGDAQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSAPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDSPLYIRSHSKAVLRASIRSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_023622523.1
Organism	Equus caballus(horse)
Definition	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023622523.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGACAC AAGAAGGAAA ACAGAACCTA CCTTTGGCGG TCATGACCCT 
CGTACAGCTG TTCAGCTTCG AAGCCTCAGC ACAGTGTTAA AACGCCTCAA GGAAATCATG 
GAGGGGAAAA GCCAGGACAG TGACCTGAAG CAGTACTGGA TGCCGGACAG CCAGTGCAGA 
GAGTGCTACG ACTGCAGCGA GAAGTTCACC ACCTTCCGGC GCAGGCACCA CTGCCGGCTC 
TGCGGGCAGA TTTTCTGCAG CCGCTGCTGC AATCAAGAGA TCCCTGGGAA GTTTATGGGC 
TATACAGGGG ACCTCCGAGC TTGCACATAC TGTAGAAAAA TAGCCTTAAG TTATGCTCAT 
TCCACAGACA GTAATTCCAT TGGGGAAGAC TTGAATGCTC TTTCAGATTC TGCTTCCTCT 
GTGTCTGTAC TTGATCCGAG TGAACCCCGA ACACCTGTTG GGAGTAGGAA AGCCAGCCGG 
AACATCTTTT TAGAGGATGA TTTTGCCTGG CAAAGTTTGA TTCATCCAGA CTCCTCAAAT 
ACTGCTCTTT CAACAAGACT TGTATCTGTT CAAGAGGACG CTGGGAAATC TCCTGCTCGA 
AATAGAATGG ACTTGGCAGT GCCTGCTCCA TTGGATGCTG TGTGGATTAA ATCATACATA 
CATGGCCCTA CTGGGTCAGC CAGCATTACC AACCTCTCAC TGGATCGATC TGGTTCTCCC 
CTGGTACCTT CATATGAGAC ATCTGTCAGT CCCCAGGCCA ACCGAACATA CGTGAGGACA 
GAGACCACAG AGGATGAACG CAAAATTCTT CTGGACAGCG TCCAGTTAAA AGACCTGTGG 
AAGAAAATCT GCCATCACAG TAGTGGAATG GAGTTTCAGG ATCACCGCTA CTGGCTGAGG 
ACACATCCCA ACTGCATCGT AGGGAAGGAG TTAGTCAACT GGCTAATCCG AAATGGGCAC 
ATTGCCACAA GGGCACAAGC TGTAGCAATT GGACAAGCGA TGGTGGACGG ACGGTGGCTG 
GACTGCGTGA GCCACCATGA CCAGCTCTTC AGGGATGAGT ATGCGCTCTA CAGACCCCTG 
CAGAGTACAG ACTTTTCTGA GACCCCTTCT CCGGACAGTG ACTCCGTGAA CTCTGTGGAA 
GGACACTCGG AGCCATCATG GTTTAAAGAC ATAAAGTTTG ATGACAGCGA CACAGAGCAG 
ATTGCTGAAG AAGGTGACGA TAATTTGACT AACTCTGCCA GTCCTAGCAA GCGCACGTCA 
GTCAGCAGTT TCCAGTCCAC AGTGGACAGT GACTCAGCCG CCTCCATCAG CCTGAACGTG 
GAGTTGGACA ACGTGAACTT CCACATCAAG AAGCCCTCCA AGTACCCACA CGTGCCCCCT 
CACCCTGCTG ACCAAAAAGA GTATTTGATT TCTGACAACG GAGGACAACA ACTCTCAATA 
AGTGACGCCT TCATCAAAGA ATCCTTATTT AATCGTCGAG TTGAGGAGAA ATCCAAAGAA 
CTGCCCTTTA CGCCTTTGGG CTGGCATCAT AACAATCTGG AGCTGCTGCG GGAGGAGAAT 
GGAGAGAAGC AGGCCATGGA GAGGTTGCTT TCAGCTAATC ATAACCACAT GATGGCACTG 
CTCCAACAGT TGCTCCATAG TGAGTCATTG TCACCATCTT GGAGGGACAT CATTGTATCC 
CTGGTCTGCC AGGTTGTTCA GACAGTCCGA CCTGACGTCA AGAACCGGGA TGACGACATG 
GATATCCGTC AGTTTGTCCA CGTCAAAAAA ATCCCAGGTG GAAAGAAGTT TGATTCTATG 
GTTGTCAATG GGTTCGTTTG TACCAAGAAT ATTGCACATA AAAAGATGAA TTCTTGTATT 
AAAAACCCCA AAATTCTTCT GTTGAAGTGT TCCATTGAGT ATCTCTACAG AGAAGAAACT 
AAATTTACTT GCATTGACCC TATCGTGCTT CAGGAAAGGG AATTCTTGAA GAATTATGTT 
CAGCGAATAG TGGATGTTCG ACCCACATTG GTTCTAGTTG AGAAAACGGT GTCTCGGATT 
GCCCAGGACC TGTTGCTGGA ACACGGCATC ACTTTGGTCA TTAATGTCAA ATCACAAGTT 
TTAGAACGAA TCAGTCGGAT GACCCAAGGT GATTTAGTGA TGTCGATGGA CCAGTTGCTC 
ACAAAGCCCC ACCTGGGCAC CTGTCACAAA TTTTACATGC AGATGTTTCA GCTACCTGGT 
GAACAAACCA AAACTCTGAT GTTTTTTGAA GGCTGTCCAC AGCACCTGGG CTGTACAATC 
AAGCTTAGAG GAGGCTCCGA TTATGAGCTG GCTCGAGTTA AGGAGATCCT CATATTTATG 
ATCTGTGTAG CATATCATTC TCAACTAGAA ATCTCCTTCC TCATGGATGA ATTTGCCATG 
CCTCCTACAT TAACGCAAAA CCCTTCCTTC CACTCTCTGA TTGAGGAACA GGAGGATGAC 
GAGGCTGCTC AAGAACAGCT CAGCGGAAGT CCCCTCCCCC GGGAACCTGA CTTCCCTCTG 
GAGTGTCTGG CCCCTGATGA GAGCAGTTCA CTGGAATCAA GGACTGTGTT TGGGAAGAGT 
GACCAGGAAC TTAAAGGTGC TCCAAAGGAT GCTGTCTCGT TGAAGCAGCA GGAGTTCGCC 
ACAGCAGCCT GCCCGGCCGG CGTCCCCTAT GCTCTCTTTG CATTGGTCCC AGAATCCTTG 
TTGCCACTGC ATCTGGGTGA CCAACAGGAT GCCAGGGGCA GCGAGCAGCC AGAGACTGTG 
CAGCAAACGG GTGAGCTACA GGATCCCAAA AGCCAGATGA GAGCCTTTAG AGACCCTCTG 
CAGGATGACA CTGGATTGTA TGTCACTGAG GAAGTCACCT CTTCTGAGGA TAAGCGGAAG 
ACTTACTCTT TGGCCTTTAA ACAGGAATTA AAAGACGTAA TTCTCTGTAT CTCCCCAGTG 
ATCACATTCC GAGAGCCTTT CCTTTTAACC GAGAAGGGGA TGCGATGCTC TCCCCGAGAC 
TATTTCGCAG AGCAGCTTTA CTGGTCTCCT CTCCTCAATA AAGACTTCAA AGAAATGGAG 
AGCAGGCGGA AGAAGCAGCT GCTCAGGGAT CTCTCCAGCC TCCAGGGCCT GAACGGAAGT 
GTGCAGGCCA AGTCTATCCA AGTGCTGCCC TCACACGAGC TGGTGAGCGC TAGGATTGCT 
GAGCATCTGG GTGACAGCCA GGGCCTGGGC CGGATGCTGG CTGACTACCG GGCCAGAGGA 
GGGAGAATTC AGCAGAATAC TTCAGACCCT TTTGCTCATT CAAAGGATGC ATCAGGTCCT 
TCAAGTGGCA AGTCAGGAAG CAGAACTGAG GGCGATGAAG AGAAAGGGCT GATTCCGAGT 
GACGCGGTAT GGTCAACAAA GGTGGACTGT CTGAACCCTG CCAACCACCA GAGGCTGTGC 
GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC AGCAACGCGC CCAGCGCCTG CGTCAGTCCT 
TGGATTGTAA CAATGGAATT TTATGGAAAG AATGATCTTA CACTGGGAAT ATTTTTAGAG 
AGATACTGTT TCAGGCCTTC TTACCAGTGT CCTAGCATGT TCTGTGATAC CCCCATGGTA 
CATCACATTC GGCGCTTTGT CCATGGCCAA GGCTGTGTGC AGATAATCCT GAAGGAGCTG 
GATTCTCCAG TACCTGGATA TCAGCATACA ATTCTCACCT ATTCCTGGTG CAGAATCTGC 
AAACAGGTAA CACCAGTTGT TGCTCTTTCC AATGAGTCCT GGTCCATGTC ATTTGCCAAA 
TACCTTGAAC TTCGGTTTTA TGGGCACCAG TACACCCGTA GAGCCAATGC TGAGCCGTGT 
GGCCACTCCA TCCACCATGA TTATCACCAG TACTTCTCCT ATAACCAGAT GGTGGCATCT 
TTTAGTTATT CTCCCATTCG GCTTCTTGAA GTATGTGTTC CACTCCCCAA AATATTCATT 
AAACGTCAGG CCCCATTAAA AGTGTCCCTT CTTCAAGATC TGAAGGACTT CTTTCAAAAA 
GTTTCACAAG TATATCTTGC TGTTGATGAA AGACTTGCAT CTTTGAAAAC TGATACATTT 
AGCAAAACAA GAGAGGAAAA AATGGAAGAT ATCTTTGCAC AAAAAGAGAT GGAGGAAGGC 
GAGTTCAAGA ACTGGATTGA GAAAATGCAA GCGAGGCTCC TGTCTTCCTC GGTAGACACC 
CCTCAGCAGC TGCAGTCGGT CCTGGAGTCG CTCATCGCCA AGAAGCAGAG CCTCTGTGAA 
GTGCTGCAGG CCTGGAATAA CAGGTTGCAG GACCTTTTCC AGCAGGAAAA GGGTAGAAAG 
CGGCCTTCAG TTCCTCCAAG TCCTGGAAGA CTGAGACAAG GGGAAGAAAG CAAGATAAGT 
GCAATGGATG CATCTCCACG GAATATTTCT CCAGGACTTC AAAATGGAGA AAAAGAGGAC 
CGCTTCTTGA CAACCCTCTC TAGTCAGGGC CCCACCTCTG CTGCTCATCT CCAGCTGCCC 
AGTCCCCCTG AAGTCCTGCC TGAGCAGGCA GGGGGGGCGA CCCCCGAGCC GGATGCAGCC 
AGCAGTTCTG AAGATGTGTT TGATGGACAT TCTCTGGGAT CCACAGACAG CCAGGTGAAG 
GAAAAATCAA CCATGAAGGC CATCTTTGCA AATTTGCTTC CAGGAAATAG CTATAACCCT 
ATTCCGTTTC CTTTTGATCC AGATAAACAC TACTTAATGT ACGAACACGA ACGGGTGCCC 
ATTGCCGTCT GTGAGAGGGA GCCCAGCTCC ATCATTGCTT TTGCTCTCAG TTGTAAAGAA 
TACCGAAATG CCTTAGAGGA ATTGTCCAAG GCAACTCTGC GGAACAGTGC CGAAGAAGGG 
CTCCCAGCCA ACAGTGCTTC AGACAGCAGA CCAAAGGGCG GCAGCCCTGG GAGGCCGCCT 
GAGCTCGGTG GAGGCTGCCC CAACCGGGCG GCCGAGGCAG AGCCGCAGCC AGCCAAAAAG 
GCTTCTGGGA TGTTGTCCTT CTTCCGAGGG ACAGCAGGGA AAAGCCCTGA TCTCTCTTCC 
CAGAAGAGAG AGACCTTACG TGGAGCAGAT AGTGCTTACT ACCAGGTCGG GCAGCCCGGC 
AAGGAGGGGA CGGAGAGTCA AGGCACTGAG CCTCCAGATG AGATAGATGG AGGAGACGCA 
CAAAAGAAAC AGCTCACAAA TCCTCATGTG GAACTTCAAT TTTCTGACGC TAACGCCAAG 
TTTTACTGCC GGCTCTACTA TGCGGGAGAG TTTCACAAGA TGCGCGAGGT GATCCTGGGC 
AGCAGCGAGG AGGACTTCAT CCGCTCCCTT TCCCACTCGG CACCCTGGCA GGCACGCGGG 
GGCAAGTCCG GAGCGGCCTT CTACGCCACG GAAGATGATA GATTCATTTT GAAGCAGATG 
CCTCGTCTGG AAGTCCAGTC TTTCCTCGAC TTTGCACCAC ACTACTTCAA TTATATCACA 
AACGCTGTGC AACAAAAGAG GCCTACTGCA CTGGCCAAAA TTCTTGGAGT TTACAGAATT 
GGTTATAAGA ATTCTCAGAA CAACACTGAG AAGAAGTTAG ATCTCCTTGT CATGGAAAAT 
CTTTTCTATG GGAGAAAGAT GGCACAGGTT TTTGACTTGA AGGGTTCACT TAGGAACAGA 
AATGTAAAAA CTGACACCGG GAAAGAGAGC TGTGACGTGG TCCTGCTGGA CGAGAATCTG 
CTGAAGATGG TCCGGGACAG CCCTCTGTAC ATCCGCTCGC ACTCCAAGGC GGTGCTGCGG 
GCCTCCATCC GCAGCGATGC CCACTTCCTG TCCAGCCACC TCATCATCGA CTATTCTTTG 
CTGGTGGGGC GAGATGATAC CAGCAATGAG CTTGTCGTTG GGATTATAGA TTATATTCGA 
ACGTTTACAT GGGACAAAAA GCTTGAGATG GTTGTGAAAT CAACAGGAAT TTTAGGAGGA 
CAAGGTAAAA TGCCAACTGT GGTCTCTCCA GAGTTGTACA GGACCCGATT TTGTGAAGCG 
ATGGACAAGT ATTTCCTCAT GGTACCAGAC CACTGGACAG GCTTGGGTCT CAACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_023622524.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023478292.1 Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X8, mRNA.

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CloneID	OHb52473
Clone ID Related Accession (Same CDS sequence)	XM_023622524.1
Accession Version	XM_023622524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGCAGA GAACAGTTTG CAGCACCCCC AGGAGAACAC AGACACAAGA 
AGGAAAACAG AACCTACCTT TGGCGGTCAT GACCCTCGTA CAGCTGTTCA GCTTCGAAGC 
CTCAGCACAG TGTTAAAACG CCTCAAGGAA ATCATGGAGG GGAAAAGCCA GGACAGTGAC 
CTGAAGCAGT ACTGGATGCC GGACAGCCAG TGCAGAGAGT GCTACGACTG CAGCGAGAAG 
TTCACCACCT TCCGGCGCAG GCACCACTGC CGGCTCTGCG GGCAGATTTT CTGCAGCCGC 
TGCTGCAATC AAGAGATCCC TGGGAAGTTT ATGGGCTATA CAGGGGACCT CCGAGCTTGC 
ACATACTGTA GAAAAATAGC CTTAAGTTAT GCTCATTCCA CAGACAGTAA TTCCATTGGG 
GAAGACTTGA ATGCTCTTTC AGATTCTGCT TCCTCTGTGT CTGTACTTGA TCCGAGTGAA 
CCCCGAACAC CTGTTGGGAG TAGGAAAGCC AGCCGGAACA TCTTTTTAGA GGATGATTTT 
GCCTGGCAAA GTTTGATTCA TCCAGACTCC TCAAATACTG CTCTTTCAAC AAGACTTGTA 
TCTGTTCAAG AGGACGCTGG GAAATCTCCT GCTCGAAATA GGTCAGCCAG CATTACCAAC 
CTCTCACTGG ATCGATCTGG TTCTCCCCTG GTACCTTCAT ATGAGACATC TGTCAGTCCC 
CAGGCCAACC GAACATACGT GAGGACAGAG ACCACAGAGG ATGAACGCAA AATTCTTCTG 
GACAGCGTCC AGTTAAAAGA CCTGTGGAAG AAAATCTGCC ATCACAGTAG TGGAATGGAG 
TTTCAGGATC ACCGCTACTG GCTGAGGACA CATCCCAACT GCATCGTAGG GAAGGAGTTA 
GTCAACTGGC TAATCCGAAA TGGGCACATT GCCACAAGGG CACAAGCTGT AGCAATTGGA 
CAAGCGATGG TGGACGGACG GTGGCTGGAC TGCGTGAGCC ACCATGACCA GCTCTTCAGG 
GATGAGTATG CGCTCTACAG ACCCCTGCAG AGTACAGACT TTTCTGAGAC CCCTTCTCCG 
GACAGTGACT CCGTGAACTC TGTGGAAGGA CACTCGGAGC CATCATGGTT TAAAGACATA 
AAGTTTGATG ACAGCGACAC AGAGCAGATT GCTGAAGAAG GTGACGATAA TTTGACTAAC 
TCTGCCAGTC CTAGCAAGCG CACGTCAGTC AGCAGTTTCC AGTCCACAGT GGACAGTGAC 
TCAGCCGCCT CCATCAGCCT GAACGTGGAG TTGGACAACG TGAACTTCCA CATCAAGAAG 
CCCTCCAAGT ACCCACACGT GCCCCCTCAC CCTGCTGACC AAAAAGAGTA TTTGATTTCT 
GACAACGGAG GACAACAACT CTCAATAAGT GACGCCTTCA TCAAAGAATC CTTATTTAAT 
CGTCGAGTTG AGGAGAAATC CAAAGAACTG CCCTTTACGC CTTTGGGCTG GCATCATAAC 
AATCTGGAGC TGCTGCGGGA GGAGAATGGA GAGAAGCAGG CCATGGAGAG GTTGCTTTCA 
GCTAATCATA ACCACATGAT GGCACTGCTC CAACAGTTGC TCCATAGTGA GTCATTGTCA 
CCATCTTGGA GGGACATCAT TGTATCCCTG GTCTGCCAGG TTGTTCAGAC AGTCCGACCT 
GACGTCAAGA ACCGGGATGA CGACATGGAT ATCCGTCAGT TTGTCCACGT CAAAAAAATC 
CCAGGTGGAA AGAAGTTTGA TTCTATGGTT GTCAATGGGT TCGTTTGTAC CAAGAATATT 
GCACATAAAA AGATGAATTC TTGTATTAAA AACCCCAAAA TTCTTCTGTT GAAGTGTTCC 
ATTGAGTATC TCTACAGAGA AGAAACTAAA TTTACTTGCA TTGACCCTAT CGTGCTTCAG 
GAAAGGGAAT TCTTGAAGAA TTATGTTCAG CGAATAGTGG ATGTTCGACC CACATTGGTT 
CTAGTTGAGA AAACGGTGTC TCGGATTGCC CAGGACCTGT TGCTGGAACA CGGCATCACT 
TTGGTCATTA ATGTCAAATC ACAAGTTTTA GAACGAATCA GTCGGATGAC CCAAGGTGAT 
TTAGTGATGT CGATGGACCA GTTGCTCACA AAGCCCCACC TGGGCACCTG TCACAAATTT 
TACATGCAGA TGTTTCAGCT ACCTGGTGAA CAAACCAAAA CTCTGATGTT TTTTGAAGGC 
TGTCCACAGC ACCTGGGCTG TACAATCAAG CTTAGAGGAG GCTCCGATTA TGAGCTGGCT 
CGAGTTAAGG AGATCCTCAT ATTTATGATC TGTGTAGCAT ATCATTCTCA ACTAGAAATC 
TCCTTCCTCA TGGATGAATT TGCCATGCCT CCTACATTAA CGCAAAACCC TTCCTTCCAC 
TCTCTGATTG AGGAACAGGA GGATGACGAG GCTGCTCAAG AACAGCTCAG CGGAAGTCCC 
CTCCCCCGGG AACCTGACTT CCCTCTGGAG TGTCTGGCCC CTGATGAGAG CAGTTCACTG 
GAATCAAGGA CTGTGTTTGG GAAGAGTGAC CAGGAACTTA AAGGTGCTCC AAAGGATGCT 
GTCTCGTTGA AGCAGCAGGA GTTCGCCACA GCAGCCTGCC CGGCCGGCGT CCCCTATGCT 
CTCTTTGCAT TGGTCCCAGA ATCCTTGTTG CCACTGCATC TGGGTGACCA ACAGGATGCC 
AGGGGCAGCG AGCAGCCAGA GACTGTGCAG CAAACGGGTG AGCTACAGGA TCCCAAAAGC 
CAGATGAGAG CCTTTAGAGA CCCTCTGCAG GATGACACTG GATTGTATGT CACTGAGGAA 
GTCACCTCTT CTGAGGATAA GCGGAAGACT TACTCTTTGG CCTTTAAACA GGAATTAAAA 
GACGTAATTC TCTGTATCTC CCCAGTGATC ACATTCCGAG AGCCTTTCCT TTTAACCGAG 
AAGGGGATGC GATGCTCTCC CCGAGACTAT TTCGCAGAGC AGCTTTACTG GTCTCCTCTC 
CTCAATAAAG ACTTCAAAGA AATGGAGAGC AGGCGGAAGA AGCAGCTGCT CAGGGATCTC 
TCCAGCCTCC AGGGCCTGAA CGGAAGTGTG CAGGCCAAGT CTATCCAAGT GCTGCCCTCA 
CACGAGCTGG TGAGCGCTAG GATTGCTGAG CATCTGGGTG ACAGCCAGGG CCTGGGCCGG 
ATGCTGGCTG ACTACCGGGC CAGAGGAGGG AGAATTCAGC AGAATACTTC AGACCCTTTT 
GCTCATTCAA AGGATGCATC AGGTCCTTCA AGTGGCAAGT CAGGAAGCAG AACTGAGGGC 
GATGAAGAGA AAGGGCTGAT TCCGAGTGAC GCGGTATGGT CAACAAAGGT GGACTGTCTG 
AACCCTGCCA ACCACCAGAG GCTGTGCGTG CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC 
AACGCGCCCA GCGCCTGCGT CAGTCCTTGG ATTGTAACAA TGGAATTTTA TGGAAAGAAT 
GATCTTACAC TGGGAATATT TTTAGAGAGA TACTGTTTCA GGCCTTCTTA CCAGTGTCCT 
AGCATGTTCT GTGATACCCC CATGGTACAT CACATTCGGC GCTTTGTCCA TGGCCAAGGC 
TGTGTGCAGA TAATCCTGAA GGAGCTGGAT TCTCCAGTAC CTGGATATCA GCATACAATT 
CTCACCTATT CCTGGTGCAG AATCTGCAAA CAGGTAACAC CAGTTGTTGC TCTTTCCAAT 
GAGTCCTGGT CCATGTCATT TGCCAAATAC CTTGAACTTC GGTTTTATGG GCACCAGTAC 
ACCCGTAGAG CCAATGCTGA GCCGTGTGGC CACTCCATCC ACCATGATTA TCACCAGTAC 
TTCTCCTATA ACCAGATGGT GGCATCTTTT AGTTATTCTC CCATTCGGCT TCTTGAAGTA 
TGTGTTCCAC TCCCCAAAAT ATTCATTAAA CGTCAGGCCC CATTAAAAGT GTCCCTTCTT 
CAAGATCTGA AGGACTTCTT TCAAAAAGTT TCACAAGTAT ATCTTGCTGT TGATGAAAGA 
CTTGCATCTT TGAAAACTGA TACATTTAGC AAAACAAGAG AGGAAAAAAT GGAAGATATC 
TTTGCACAAA AAGAGATGGA GGAAGGCGAG TTCAAGAACT GGATTGAGAA AATGCAAGCG 
AGGCTCCTGT CTTCCTCGGT AGACACCCCT CAGCAGCTGC AGTCGGTCCT GGAGTCGCTC 
ATCGCCAAGA AGCAGAGCCT CTGTGAAGTG CTGCAGGCCT GGAATAACAG GTTGCAGGAC 
CTTTTCCAGC AGGAAAAGGG TAGAAAGCGG CCTTCAGTTC CTCCAAGTCC TGGAAGACTG 
AGACAAGGGG AAGAAAGCAA GATAAGTGCA ATGGATGCAT CTCCACGGAA TATTTCTCCA 
GGACTTCAAA ATGGAGAAAA AGAGGACCGC TTCTTGACAA CCCTCTCTAG TCAGGGCCCC 
ACCTCTGCTG CTCATCTCCA GCTGCCCAGT CCCCCTGAAG TCCTGCCTGA GCAGGCAGGG 
GGGGCGACCC CCGAGCCGGA TGCAGCCAGC AGTTCTGAAG ATGTGTTTGA TGGACATTCT 
CTGGGATCCA CAGACAGCCA GGTGAAGGAA AAATCAACCA TGAAGGCCAT CTTTGCAAAT 
TTGCTTCCAG GAAATAGCTA TAACCCTATT CCGTTTCCTT TTGATCCAGA TAAACACTAC 
TTAATGTACG AACACGAACG GGTGCCCATT GCCGTCTGTG AGAGGGAGCC CAGCTCCATC 
ATTGCTTTTG CTCTCAGTTG TAAAGAATAC CGAAATGCCT TAGAGGAATT GTCCAAGGCA 
ACTCTGCGGA ACAGTGCCGA AGAAGGGCTC CCAGCCAACA GTGCTTCAGA CAGCAGACCA 
AAGGGCGGCA GCCCTGGGAG GCCGCCTGAG CTCGGTGGAG GCTGCCCCAA CCGGGCGGCC 
GAGGCAGAGC CGCAGCCAGC CAAAAAGGCT TCTGGGATGT TGTCCTTCTT CCGAGGGACA 
GCAGGGAAAA GCCCTGATCT CTCTTCCCAG AAGAGAGAGA CCTTACGTGG AGCAGATAGT 
GCTTACTACC AGGTCGGGCA GCCCGGCAAG GAGGGGACGG AGAGTCAAGG CACTGAGCCT 
CCAGATGAGA TAGATGGAGG AGACGCACAA AAGAAACAGC TCACAAATCC TCATGTGGAA 
CTTCAATTTT CTGACGCTAA CGCCAAGTTT TACTGCCGGC TCTACTATGC GGGAGAGTTT 
CACAAGATGC GCGAGGTGAT CCTGGGCAGC AGCGAGGAGG ACTTCATCCG CTCCCTTTCC 
CACTCGGCAC CCTGGCAGGC ACGCGGGGGC AAGTCCGGAG CGGCCTTCTA CGCCACGGAA 
GATGATAGAT TCATTTTGAA GCAGATGCCT CGTCTGGAAG TCCAGTCTTT CCTCGACTTT 
GCACCACACT ACTTCAATTA TATCACAAAC GCTGTGCAAC AAAAGAGGCC TACTGCACTG 
GCCAAAATTC TTGGAGTTTA CAGAATTGGT TATAAGAATT CTCAGAACAA CACTGAGAAG 
AAGTTAGATC TCCTTGTCAT GGAAAATCTT TTCTATGGGA GAAAGATGGC ACAGGTTTTT 
GACTTGAAGG GTTCACTTAG GAACAGAAAT GTAAAAACTG ACACCGGGAA AGAGAGCTGT 
GACGTGGTCC TGCTGGACGA GAATCTGCTG AAGATGGTCC GGGACAGCCC TCTGTACATC 
CGCTCGCACT CCAAGGCGGT GCTGCGGGCC TCCATCCGCA GCGATGCCCA CTTCCTGTCC 
AGCCACCTCA TCATCGACTA TTCTTTGCTG GTGGGGCGAG ATGATACCAG CAATGAGCTT 
GTCGTTGGGA TTATAGATTA TATTCGAACG TTTACATGGG ACAAAAAGCT TGAGATGGTT 
GTGAAATCAA CAGGAATTTT AGGAGGACAA GGTAAAATGC CAACTGTGGT CTCTCCAGAG 
TTGTACAGGA CCCGATTTTG TGAAGCGATG GACAAGTATT TCCTCATGGT ACCAGACCAC 
TGGACAGGCT TGGGTCTCAA CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023478292.1
CDS	114..6440
Translation	MATDDKTSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPAFKSAYSSFVNLFRFNKDRAEGEQQSLTGGWPSPQLPSRTQSVRSPIPYKKQLTEELQRRPSAVLAENSLQHPQENTDTRRKTEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCRECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPLVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAVAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTDFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSPSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHVKKIPGGKKFDSMVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDLLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPGEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEEQEDDEAAQEQLSGSPLPREPDFPLECLAPDESSSLESRTVFGKSDQELKGAPKDAVSLKQQEFATAACPAGVPYALFALVPESLLPLHLGDQQDARGSEQPETVQQTGELQDPKSQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSPRDYFAEQLYWSPLLNKDFKEMESRRKKQLLRDLSSLQGLNGSVQAKSIQVLPSHELVSARIAEHLGDSQGLGRMLADYRARGGRIQQNTSDPFAHSKDASGPSSGKSGSRTEGDEEKGLIPSDAVWSTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLLSSSVDTPQQLQSVLESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQGPTSAAHLQLPSPPEVLPEQAGGATPEPDAASSSEDVFDGHSLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSASDSRPKGGSPGRPPELGGGCPNRAAEAEPQPAKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQPGKEGTESQGTEPPDEIDGGDAQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSAPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDSPLYIRSHSKAVLRASIRSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_023622524.1
Organism	Equus caballus(horse)
Definition	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023622524.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGCAGA GAACAGTTTG CAGCACCCCC AGGAGAACAC AGACACAAGA 
AGGAAAACAG AACCTACCTT TGGCGGTCAT GACCCTCGTA CAGCTGTTCA GCTTCGAAGC 
CTCAGCACAG TGTTAAAACG CCTCAAGGAA ATCATGGAGG GGAAAAGCCA GGACAGTGAC 
CTGAAGCAGT ACTGGATGCC GGACAGCCAG TGCAGAGAGT GCTACGACTG CAGCGAGAAG 
TTCACCACCT TCCGGCGCAG GCACCACTGC CGGCTCTGCG GGCAGATTTT CTGCAGCCGC 
TGCTGCAATC AAGAGATCCC TGGGAAGTTT ATGGGCTATA CAGGGGACCT CCGAGCTTGC 
ACATACTGTA GAAAAATAGC CTTAAGTTAT GCTCATTCCA CAGACAGTAA TTCCATTGGG 
GAAGACTTGA ATGCTCTTTC AGATTCTGCT TCCTCTGTGT CTGTACTTGA TCCGAGTGAA 
CCCCGAACAC CTGTTGGGAG TAGGAAAGCC AGCCGGAACA TCTTTTTAGA GGATGATTTT 
GCCTGGCAAA GTTTGATTCA TCCAGACTCC TCAAATACTG CTCTTTCAAC AAGACTTGTA 
TCTGTTCAAG AGGACGCTGG GAAATCTCCT GCTCGAAATA GGTCAGCCAG CATTACCAAC 
CTCTCACTGG ATCGATCTGG TTCTCCCCTG GTACCTTCAT ATGAGACATC TGTCAGTCCC 
CAGGCCAACC GAACATACGT GAGGACAGAG ACCACAGAGG ATGAACGCAA AATTCTTCTG 
GACAGCGTCC AGTTAAAAGA CCTGTGGAAG AAAATCTGCC ATCACAGTAG TGGAATGGAG 
TTTCAGGATC ACCGCTACTG GCTGAGGACA CATCCCAACT GCATCGTAGG GAAGGAGTTA 
GTCAACTGGC TAATCCGAAA TGGGCACATT GCCACAAGGG CACAAGCTGT AGCAATTGGA 
CAAGCGATGG TGGACGGACG GTGGCTGGAC TGCGTGAGCC ACCATGACCA GCTCTTCAGG 
GATGAGTATG CGCTCTACAG ACCCCTGCAG AGTACAGACT TTTCTGAGAC CCCTTCTCCG 
GACAGTGACT CCGTGAACTC TGTGGAAGGA CACTCGGAGC CATCATGGTT TAAAGACATA 
AAGTTTGATG ACAGCGACAC AGAGCAGATT GCTGAAGAAG GTGACGATAA TTTGACTAAC 
TCTGCCAGTC CTAGCAAGCG CACGTCAGTC AGCAGTTTCC AGTCCACAGT GGACAGTGAC 
TCAGCCGCCT CCATCAGCCT GAACGTGGAG TTGGACAACG TGAACTTCCA CATCAAGAAG 
CCCTCCAAGT ACCCACACGT GCCCCCTCAC CCTGCTGACC AAAAAGAGTA TTTGATTTCT 
GACAACGGAG GACAACAACT CTCAATAAGT GACGCCTTCA TCAAAGAATC CTTATTTAAT 
CGTCGAGTTG AGGAGAAATC CAAAGAACTG CCCTTTACGC CTTTGGGCTG GCATCATAAC 
AATCTGGAGC TGCTGCGGGA GGAGAATGGA GAGAAGCAGG CCATGGAGAG GTTGCTTTCA 
GCTAATCATA ACCACATGAT GGCACTGCTC CAACAGTTGC TCCATAGTGA GTCATTGTCA 
CCATCTTGGA GGGACATCAT TGTATCCCTG GTCTGCCAGG TTGTTCAGAC AGTCCGACCT 
GACGTCAAGA ACCGGGATGA CGACATGGAT ATCCGTCAGT TTGTCCACGT CAAAAAAATC 
CCAGGTGGAA AGAAGTTTGA TTCTATGGTT GTCAATGGGT TCGTTTGTAC CAAGAATATT 
GCACATAAAA AGATGAATTC TTGTATTAAA AACCCCAAAA TTCTTCTGTT GAAGTGTTCC 
ATTGAGTATC TCTACAGAGA AGAAACTAAA TTTACTTGCA TTGACCCTAT CGTGCTTCAG 
GAAAGGGAAT TCTTGAAGAA TTATGTTCAG CGAATAGTGG ATGTTCGACC CACATTGGTT 
CTAGTTGAGA AAACGGTGTC TCGGATTGCC CAGGACCTGT TGCTGGAACA CGGCATCACT 
TTGGTCATTA ATGTCAAATC ACAAGTTTTA GAACGAATCA GTCGGATGAC CCAAGGTGAT 
TTAGTGATGT CGATGGACCA GTTGCTCACA AAGCCCCACC TGGGCACCTG TCACAAATTT 
TACATGCAGA TGTTTCAGCT ACCTGGTGAA CAAACCAAAA CTCTGATGTT TTTTGAAGGC 
TGTCCACAGC ACCTGGGCTG TACAATCAAG CTTAGAGGAG GCTCCGATTA TGAGCTGGCT 
CGAGTTAAGG AGATCCTCAT ATTTATGATC TGTGTAGCAT ATCATTCTCA ACTAGAAATC 
TCCTTCCTCA TGGATGAATT TGCCATGCCT CCTACATTAA CGCAAAACCC TTCCTTCCAC 
TCTCTGATTG AGGAACAGGA GGATGACGAG GCTGCTCAAG AACAGCTCAG CGGAAGTCCC 
CTCCCCCGGG AACCTGACTT CCCTCTGGAG TGTCTGGCCC CTGATGAGAG CAGTTCACTG 
GAATCAAGGA CTGTGTTTGG GAAGAGTGAC CAGGAACTTA AAGGTGCTCC AAAGGATGCT 
GTCTCGTTGA AGCAGCAGGA GTTCGCCACA GCAGCCTGCC CGGCCGGCGT CCCCTATGCT 
CTCTTTGCAT TGGTCCCAGA ATCCTTGTTG CCACTGCATC TGGGTGACCA ACAGGATGCC 
AGGGGCAGCG AGCAGCCAGA GACTGTGCAG CAAACGGGTG AGCTACAGGA TCCCAAAAGC 
CAGATGAGAG CCTTTAGAGA CCCTCTGCAG GATGACACTG GATTGTATGT CACTGAGGAA 
GTCACCTCTT CTGAGGATAA GCGGAAGACT TACTCTTTGG CCTTTAAACA GGAATTAAAA 
GACGTAATTC TCTGTATCTC CCCAGTGATC ACATTCCGAG AGCCTTTCCT TTTAACCGAG 
AAGGGGATGC GATGCTCTCC CCGAGACTAT TTCGCAGAGC AGCTTTACTG GTCTCCTCTC 
CTCAATAAAG ACTTCAAAGA AATGGAGAGC AGGCGGAAGA AGCAGCTGCT CAGGGATCTC 
TCCAGCCTCC AGGGCCTGAA CGGAAGTGTG CAGGCCAAGT CTATCCAAGT GCTGCCCTCA 
CACGAGCTGG TGAGCGCTAG GATTGCTGAG CATCTGGGTG ACAGCCAGGG CCTGGGCCGG 
ATGCTGGCTG ACTACCGGGC CAGAGGAGGG AGAATTCAGC AGAATACTTC AGACCCTTTT 
GCTCATTCAA AGGATGCATC AGGTCCTTCA AGTGGCAAGT CAGGAAGCAG AACTGAGGGC 
GATGAAGAGA AAGGGCTGAT TCCGAGTGAC GCGGTATGGT CAACAAAGGT GGACTGTCTG 
AACCCTGCCA ACCACCAGAG GCTGTGCGTG CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC 
AACGCGCCCA GCGCCTGCGT CAGTCCTTGG ATTGTAACAA TGGAATTTTA TGGAAAGAAT 
GATCTTACAC TGGGAATATT TTTAGAGAGA TACTGTTTCA GGCCTTCTTA CCAGTGTCCT 
AGCATGTTCT GTGATACCCC CATGGTACAT CACATTCGGC GCTTTGTCCA TGGCCAAGGC 
TGTGTGCAGA TAATCCTGAA GGAGCTGGAT TCTCCAGTAC CTGGATATCA GCATACAATT 
CTCACCTATT CCTGGTGCAG AATCTGCAAA CAGGTAACAC CAGTTGTTGC TCTTTCCAAT 
GAGTCCTGGT CCATGTCATT TGCCAAATAC CTTGAACTTC GGTTTTATGG GCACCAGTAC 
ACCCGTAGAG CCAATGCTGA GCCGTGTGGC CACTCCATCC ACCATGATTA TCACCAGTAC 
TTCTCCTATA ACCAGATGGT GGCATCTTTT AGTTATTCTC CCATTCGGCT TCTTGAAGTA 
TGTGTTCCAC TCCCCAAAAT ATTCATTAAA CGTCAGGCCC CATTAAAAGT GTCCCTTCTT 
CAAGATCTGA AGGACTTCTT TCAAAAAGTT TCACAAGTAT ATCTTGCTGT TGATGAAAGA 
CTTGCATCTT TGAAAACTGA TACATTTAGC AAAACAAGAG AGGAAAAAAT GGAAGATATC 
TTTGCACAAA AAGAGATGGA GGAAGGCGAG TTCAAGAACT GGATTGAGAA AATGCAAGCG 
AGGCTCCTGT CTTCCTCGGT AGACACCCCT CAGCAGCTGC AGTCGGTCCT GGAGTCGCTC 
ATCGCCAAGA AGCAGAGCCT CTGTGAAGTG CTGCAGGCCT GGAATAACAG GTTGCAGGAC 
CTTTTCCAGC AGGAAAAGGG TAGAAAGCGG CCTTCAGTTC CTCCAAGTCC TGGAAGACTG 
AGACAAGGGG AAGAAAGCAA GATAAGTGCA ATGGATGCAT CTCCACGGAA TATTTCTCCA 
GGACTTCAAA ATGGAGAAAA AGAGGACCGC TTCTTGACAA CCCTCTCTAG TCAGGGCCCC 
ACCTCTGCTG CTCATCTCCA GCTGCCCAGT CCCCCTGAAG TCCTGCCTGA GCAGGCAGGG 
GGGGCGACCC CCGAGCCGGA TGCAGCCAGC AGTTCTGAAG ATGTGTTTGA TGGACATTCT 
CTGGGATCCA CAGACAGCCA GGTGAAGGAA AAATCAACCA TGAAGGCCAT CTTTGCAAAT 
TTGCTTCCAG GAAATAGCTA TAACCCTATT CCGTTTCCTT TTGATCCAGA TAAACACTAC 
TTAATGTACG AACACGAACG GGTGCCCATT GCCGTCTGTG AGAGGGAGCC CAGCTCCATC 
ATTGCTTTTG CTCTCAGTTG TAAAGAATAC CGAAATGCCT TAGAGGAATT GTCCAAGGCA 
ACTCTGCGGA ACAGTGCCGA AGAAGGGCTC CCAGCCAACA GTGCTTCAGA CAGCAGACCA 
AAGGGCGGCA GCCCTGGGAG GCCGCCTGAG CTCGGTGGAG GCTGCCCCAA CCGGGCGGCC 
GAGGCAGAGC CGCAGCCAGC CAAAAAGGCT TCTGGGATGT TGTCCTTCTT CCGAGGGACA 
GCAGGGAAAA GCCCTGATCT CTCTTCCCAG AAGAGAGAGA CCTTACGTGG AGCAGATAGT 
GCTTACTACC AGGTCGGGCA GCCCGGCAAG GAGGGGACGG AGAGTCAAGG CACTGAGCCT 
CCAGATGAGA TAGATGGAGG AGACGCACAA AAGAAACAGC TCACAAATCC TCATGTGGAA 
CTTCAATTTT CTGACGCTAA CGCCAAGTTT TACTGCCGGC TCTACTATGC GGGAGAGTTT 
CACAAGATGC GCGAGGTGAT CCTGGGCAGC AGCGAGGAGG ACTTCATCCG CTCCCTTTCC 
CACTCGGCAC CCTGGCAGGC ACGCGGGGGC AAGTCCGGAG CGGCCTTCTA CGCCACGGAA 
GATGATAGAT TCATTTTGAA GCAGATGCCT CGTCTGGAAG TCCAGTCTTT CCTCGACTTT 
GCACCACACT ACTTCAATTA TATCACAAAC GCTGTGCAAC AAAAGAGGCC TACTGCACTG 
GCCAAAATTC TTGGAGTTTA CAGAATTGGT TATAAGAATT CTCAGAACAA CACTGAGAAG 
AAGTTAGATC TCCTTGTCAT GGAAAATCTT TTCTATGGGA GAAAGATGGC ACAGGTTTTT 
GACTTGAAGG GTTCACTTAG GAACAGAAAT GTAAAAACTG ACACCGGGAA AGAGAGCTGT 
GACGTGGTCC TGCTGGACGA GAATCTGCTG AAGATGGTCC GGGACAGCCC TCTGTACATC 
CGCTCGCACT CCAAGGCGGT GCTGCGGGCC TCCATCCGCA GCGATGCCCA CTTCCTGTCC 
AGCCACCTCA TCATCGACTA TTCTTTGCTG GTGGGGCGAG ATGATACCAG CAATGAGCTT 
GTCGTTGGGA TTATAGATTA TATTCGAACG TTTACATGGG ACAAAAAGCT TGAGATGGTT 
GTGAAATCAA CAGGAATTTT AGGAGGACAA GGTAAAATGC CAACTGTGGT CTCTCCAGAG 
TTGTACAGGA CCCGATTTTG TGAAGCGATG GACAAGTATT TCCTCATGGT ACCAGACCAC 
TGGACAGGCT TGGGTCTCAA CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_023622525.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023478293.1 Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X9, mRNA.

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CloneID	OHb52474
Clone ID Related Accession (Same CDS sequence)	XM_023622525.1
Accession Version	XM_023622525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGACAC AAGAAGGAAA ACAGAACCTA CCTTTGGCGG TCATGACCCT 
CGTACAGCTG TTCAGCTTCG AAGCCTCAGC ACAGTGTTAA AACGCCTCAA GGAAATCATG 
GAGGGGAAAA GCCAGGACAG TGACCTGAAG CAGTACTGGA TGCCGGACAG CCAGTGCAGA 
GAGTGCTACG ACTGCAGCGA GAAGTTCACC ACCTTCCGGC GCAGGCACCA CTGCCGGCTC 
TGCGGGCAGA TTTTCTGCAG CCGCTGCTGC AATCAAGAGA TCCCTGGGAA GTTTATGGGC 
TATACAGGGG ACCTCCGAGC TTGCACATAC TGTAGAAAAA TAGCCTTAAG TTATGCTCAT 
TCCACAGACA GTAATTCCAT TGGGGAAGAC TTGAATGCTC TTTCAGATTC TGCTTCCTCT 
GTGTCTGTAC TTGATCCGAG TGAACCCCGA ACACCTGTTG GGAGTAGGAA AGCCAGCCGG 
AACATCTTTT TAGAGGATGA TTTTGCCTGG CAAAGTTTGA TTCATCCAGA CTCCTCAAAT 
ACTGCTCTTT CAACAAGACT TGTATCTGTT CAAGAGGACG CTGGGAAATC TCCTGCTCGA 
AATAGGTCAG CCAGCATTAC CAACCTCTCA CTGGATCGAT CTGGTTCTCC CCTGGTACCT 
TCATATGAGA CATCTGTCAG TCCCCAGGCC AACCGAACAT ACGTGAGGAC AGAGACCACA 
GAGGATGAAC GCAAAATTCT TCTGGACAGC GTCCAGTTAA AAGACCTGTG GAAGAAAATC 
TGCCATCACA GTAGTGGAAT GGAGTTTCAG GATCACCGCT ACTGGCTGAG GACACATCCC 
AACTGCATCG TAGGGAAGGA GTTAGTCAAC TGGCTAATCC GAAATGGGCA CATTGCCACA 
AGGGCACAAG CTGTAGCAAT TGGACAAGCG ATGGTGGACG GACGGTGGCT GGACTGCGTG 
AGCCACCATG ACCAGCTCTT CAGGGATGAG TATGCGCTCT ACAGACCCCT GCAGAGTACA 
GACTTTTCTG AGACCCCTTC TCCGGACAGT GACTCCGTGA ACTCTGTGGA AGGACACTCG 
GAGCCATCAT GGTTTAAAGA CATAAAGTTT GATGACAGCG ACACAGAGCA GATTGCTGAA 
GAAGGTGACG ATAATTTGAC TAACTCTGCC AGTCCTAGCA AGCGCACGTC AGTCAGCAGT 
TTCCAGTCCA CAGTGGACAG TGACTCAGCC GCCTCCATCA GCCTGAACGT GGAGTTGGAC 
AACGTGAACT TCCACATCAA GAAGCCCTCC AAGTACCCAC ACGTGCCCCC TCACCCTGCT 
GACCAAAAAG AGTATTTGAT TTCTGACAAC GGAGGACAAC AACTCTCAAT AAGTGACGCC 
TTCATCAAAG AATCCTTATT TAATCGTCGA GTTGAGGAGA AATCCAAAGA ACTGCCCTTT 
ACGCCTTTGG GCTGGCATCA TAACAATCTG GAGCTGCTGC GGGAGGAGAA TGGAGAGAAG 
CAGGCCATGG AGAGGTTGCT TTCAGCTAAT CATAACCACA TGATGGCACT GCTCCAACAG 
TTGCTCCATA GTGAGTCATT GTCACCATCT TGGAGGGACA TCATTGTATC CCTGGTCTGC 
CAGGTTGTTC AGACAGTCCG ACCTGACGTC AAGAACCGGG ATGACGACAT GGATATCCGT 
CAGTTTGTCC ACGTCAAAAA AATCCCAGGT GGAAAGAAGT TTGATTCTAT GGTTGTCAAT 
GGGTTCGTTT GTACCAAGAA TATTGCACAT AAAAAGATGA ATTCTTGTAT TAAAAACCCC 
AAAATTCTTC TGTTGAAGTG TTCCATTGAG TATCTCTACA GAGAAGAAAC TAAATTTACT 
TGCATTGACC CTATCGTGCT TCAGGAAAGG GAATTCTTGA AGAATTATGT TCAGCGAATA 
GTGGATGTTC GACCCACATT GGTTCTAGTT GAGAAAACGG TGTCTCGGAT TGCCCAGGAC 
CTGTTGCTGG AACACGGCAT CACTTTGGTC ATTAATGTCA AATCACAAGT TTTAGAACGA 
ATCAGTCGGA TGACCCAAGG TGATTTAGTG ATGTCGATGG ACCAGTTGCT CACAAAGCCC 
CACCTGGGCA CCTGTCACAA ATTTTACATG CAGATGTTTC AGCTACCTGG TGAACAAACC 
AAAACTCTGA TGTTTTTTGA AGGCTGTCCA CAGCACCTGG GCTGTACAAT CAAGCTTAGA 
GGAGGCTCCG ATTATGAGCT GGCTCGAGTT AAGGAGATCC TCATATTTAT GATCTGTGTA 
GCATATCATT CTCAACTAGA AATCTCCTTC CTCATGGATG AATTTGCCAT GCCTCCTACA 
TTAACGCAAA ACCCTTCCTT CCACTCTCTG ATTGAGGAAC AGGAGGATGA CGAGGCTGCT 
CAAGAACAGC TCAGCGGAAG TCCCCTCCCC CGGGAACCTG ACTTCCCTCT GGAGTGTCTG 
GCCCCTGATG AGAGCAGTTC ACTGGAATCA AGGACTGTGT TTGGGAAGAG TGACCAGGAA 
CTTAAAGGTG CTCCAAAGGA TGCTGTCTCG TTGAAGCAGC AGGAGTTCGC CACAGCAGCC 
TGCCCGGCCG GCGTCCCCTA TGCTCTCTTT GCATTGGTCC CAGAATCCTT GTTGCCACTG 
CATCTGGGTG ACCAACAGGA TGCCAGGGGC AGCGAGCAGC CAGAGACTGT GCAGCAAACG 
GGTGAGCTAC AGGATCCCAA AAGCCAGATG AGAGCCTTTA GAGACCCTCT GCAGGATGAC 
ACTGGATTGT ATGTCACTGA GGAAGTCACC TCTTCTGAGG ATAAGCGGAA GACTTACTCT 
TTGGCCTTTA AACAGGAATT AAAAGACGTA ATTCTCTGTA TCTCCCCAGT GATCACATTC 
CGAGAGCCTT TCCTTTTAAC CGAGAAGGGG ATGCGATGCT CTCCCCGAGA CTATTTCGCA 
GAGCAGCTTT ACTGGTCTCC TCTCCTCAAT AAAGACTTCA AAGAAATGGA GAGCAGGCGG 
AAGAAGCAGC TGCTCAGGGA TCTCTCCAGC CTCCAGGGCC TGAACGGAAG TGTGCAGGCC 
AAGTCTATCC AAGTGCTGCC CTCACACGAG CTGGTGAGCG CTAGGATTGC TGAGCATCTG 
GGTGACAGCC AGGGCCTGGG CCGGATGCTG GCTGACTACC GGGCCAGAGG AGGGAGAATT 
CAGCAGAATA CTTCAGACCC TTTTGCTCAT TCAAAGGATG CATCAGGTCC TTCAAGTGGC 
AAGTCAGGAA GCAGAACTGA GGGCGATGAA GAGAAAGGGC TGATTCCGAG TGACGCGGTA 
TGGTCAACAA AGGTGGACTG TCTGAACCCT GCCAACCACC AGAGGCTGTG CGTGCTCTTC 
AGCAGCTCTT CTGCCCAGTC CAGCAACGCG CCCAGCGCCT GCGTCAGTCC TTGGATTGTA 
ACAATGGAAT TTTATGGAAA GAATGATCTT ACACTGGGAA TATTTTTAGA GAGATACTGT 
TTCAGGCCTT CTTACCAGTG TCCTAGCATG TTCTGTGATA CCCCCATGGT ACATCACATT 
CGGCGCTTTG TCCATGGCCA AGGCTGTGTG CAGATAATCC TGAAGGAGCT GGATTCTCCA 
GTACCTGGAT ATCAGCATAC AATTCTCACC TATTCCTGGT GCAGAATCTG CAAACAGGTA 
ACACCAGTTG TTGCTCTTTC CAATGAGTCC TGGTCCATGT CATTTGCCAA ATACCTTGAA 
CTTCGGTTTT ATGGGCACCA GTACACCCGT AGAGCCAATG CTGAGCCGTG TGGCCACTCC 
ATCCACCATG ATTATCACCA GTACTTCTCC TATAACCAGA TGGTGGCATC TTTTAGTTAT 
TCTCCCATTC GGCTTCTTGA AGTATGTGTT CCACTCCCCA AAATATTCAT TAAACGTCAG 
GCCCCATTAA AAGTGTCCCT TCTTCAAGAT CTGAAGGACT TCTTTCAAAA AGTTTCACAA 
GTATATCTTG CTGTTGATGA AAGACTTGCA TCTTTGAAAA CTGATACATT TAGCAAAACA 
AGAGAGGAAA AAATGGAAGA TATCTTTGCA CAAAAAGAGA TGGAGGAAGG CGAGTTCAAG 
AACTGGATTG AGAAAATGCA AGCGAGGCTC CTGTCTTCCT CGGTAGACAC CCCTCAGCAG 
CTGCAGTCGG TCCTGGAGTC GCTCATCGCC AAGAAGCAGA GCCTCTGTGA AGTGCTGCAG 
GCCTGGAATA ACAGGTTGCA GGACCTTTTC CAGCAGGAAA AGGGTAGAAA GCGGCCTTCA 
GTTCCTCCAA GTCCTGGAAG ACTGAGACAA GGGGAAGAAA GCAAGATAAG TGCAATGGAT 
GCATCTCCAC GGAATATTTC TCCAGGACTT CAAAATGGAG AAAAAGAGGA CCGCTTCTTG 
ACAACCCTCT CTAGTCAGGG CCCCACCTCT GCTGCTCATC TCCAGCTGCC CAGTCCCCCT 
GAAGTCCTGC CTGAGCAGGC AGGGGGGGCG ACCCCCGAGC CGGATGCAGC CAGCAGTTCT 
GAAGATGTGT TTGATGGACA TTCTCTGGGA TCCACAGACA GCCAGGTGAA GGAAAAATCA 
ACCATGAAGG CCATCTTTGC AAATTTGCTT CCAGGAAATA GCTATAACCC TATTCCGTTT 
CCTTTTGATC CAGATAAACA CTACTTAATG TACGAACACG AACGGGTGCC CATTGCCGTC 
TGTGAGAGGG AGCCCAGCTC CATCATTGCT TTTGCTCTCA GTTGTAAAGA ATACCGAAAT 
GCCTTAGAGG AATTGTCCAA GGCAACTCTG CGGAACAGTG CCGAAGAAGG GCTCCCAGCC 
AACAGTGCTT CAGACAGCAG ACCAAAGGGC GGCAGCCCTG GGAGGCCGCC TGAGCTCGGT 
GGAGGCTGCC CCAACCGGGC GGCCGAGGCA GAGCCGCAGC CAGCCAAAAA GGCTTCTGGG 
ATGTTGTCCT TCTTCCGAGG GACAGCAGGG AAAAGCCCTG ATCTCTCTTC CCAGAAGAGA 
GAGACCTTAC GTGGAGCAGA TAGTGCTTAC TACCAGGTCG GGCAGCCCGG CAAGGAGGGG 
ACGGAGAGTC AAGGCACTGA GCCTCCAGAT GAGATAGATG GAGGAGACGC ACAAAAGAAA 
CAGCTCACAA ATCCTCATGT GGAACTTCAA TTTTCTGACG CTAACGCCAA GTTTTACTGC 
CGGCTCTACT ATGCGGGAGA GTTTCACAAG ATGCGCGAGG TGATCCTGGG CAGCAGCGAG 
GAGGACTTCA TCCGCTCCCT TTCCCACTCG GCACCCTGGC AGGCACGCGG GGGCAAGTCC 
GGAGCGGCCT TCTACGCCAC GGAAGATGAT AGATTCATTT TGAAGCAGAT GCCTCGTCTG 
GAAGTCCAGT CTTTCCTCGA CTTTGCACCA CACTACTTCA ATTATATCAC AAACGCTGTG 
CAACAAAAGA GGCCTACTGC ACTGGCCAAA ATTCTTGGAG TTTACAGAAT TGGTTATAAG 
AATTCTCAGA ACAACACTGA GAAGAAGTTA GATCTCCTTG TCATGGAAAA TCTTTTCTAT 
GGGAGAAAGA TGGCACAGGT TTTTGACTTG AAGGGTTCAC TTAGGAACAG AAATGTAAAA 
ACTGACACCG GGAAAGAGAG CTGTGACGTG GTCCTGCTGG ACGAGAATCT GCTGAAGATG 
GTCCGGGACA GCCCTCTGTA CATCCGCTCG CACTCCAAGG CGGTGCTGCG GGCCTCCATC 
CGCAGCGATG CCCACTTCCT GTCCAGCCAC CTCATCATCG ACTATTCTTT GCTGGTGGGG 
CGAGATGATA CCAGCAATGA GCTTGTCGTT GGGATTATAG ATTATATTCG AACGTTTACA 
TGGGACAAAA AGCTTGAGAT GGTTGTGAAA TCAACAGGAA TTTTAGGAGG ACAAGGTAAA 
ATGCCAACTG TGGTCTCTCC AGAGTTGTAC AGGACCCGAT TTTGTGAAGC GATGGACAAG 
TATTTCCTCA TGGTACCAGA CCACTGGACA GGCTTGGGTC TCAACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023478293.1
CDS	114..6404
Translation	MATDDKTSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPAFKSAYSSFVNLFRFNKDRAEGEQQSLTGGWPSPQLPSRTQSVRSPIPYKKQLTEELQRRPSAVLDTRRKTEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCRECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPLVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAVAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTDFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSPSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHVKKIPGGKKFDSMVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDLLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPGEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEEQEDDEAAQEQLSGSPLPREPDFPLECLAPDESSSLESRTVFGKSDQELKGAPKDAVSLKQQEFATAACPAGVPYALFALVPESLLPLHLGDQQDARGSEQPETVQQTGELQDPKSQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSPRDYFAEQLYWSPLLNKDFKEMESRRKKQLLRDLSSLQGLNGSVQAKSIQVLPSHELVSARIAEHLGDSQGLGRMLADYRARGGRIQQNTSDPFAHSKDASGPSSGKSGSRTEGDEEKGLIPSDAVWSTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLLSSSVDTPQQLQSVLESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQGPTSAAHLQLPSPPEVLPEQAGGATPEPDAASSSEDVFDGHSLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSASDSRPKGGSPGRPPELGGGCPNRAAEAEPQPAKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQPGKEGTESQGTEPPDEIDGGDAQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSAPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDSPLYIRSHSKAVLRASIRSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_023622525.1
Organism	Equus caballus(horse)
Definition	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023622525.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGACAC AAGAAGGAAA ACAGAACCTA CCTTTGGCGG TCATGACCCT 
CGTACAGCTG TTCAGCTTCG AAGCCTCAGC ACAGTGTTAA AACGCCTCAA GGAAATCATG 
GAGGGGAAAA GCCAGGACAG TGACCTGAAG CAGTACTGGA TGCCGGACAG CCAGTGCAGA 
GAGTGCTACG ACTGCAGCGA GAAGTTCACC ACCTTCCGGC GCAGGCACCA CTGCCGGCTC 
TGCGGGCAGA TTTTCTGCAG CCGCTGCTGC AATCAAGAGA TCCCTGGGAA GTTTATGGGC 
TATACAGGGG ACCTCCGAGC TTGCACATAC TGTAGAAAAA TAGCCTTAAG TTATGCTCAT 
TCCACAGACA GTAATTCCAT TGGGGAAGAC TTGAATGCTC TTTCAGATTC TGCTTCCTCT 
GTGTCTGTAC TTGATCCGAG TGAACCCCGA ACACCTGTTG GGAGTAGGAA AGCCAGCCGG 
AACATCTTTT TAGAGGATGA TTTTGCCTGG CAAAGTTTGA TTCATCCAGA CTCCTCAAAT 
ACTGCTCTTT CAACAAGACT TGTATCTGTT CAAGAGGACG CTGGGAAATC TCCTGCTCGA 
AATAGGTCAG CCAGCATTAC CAACCTCTCA CTGGATCGAT CTGGTTCTCC CCTGGTACCT 
TCATATGAGA CATCTGTCAG TCCCCAGGCC AACCGAACAT ACGTGAGGAC AGAGACCACA 
GAGGATGAAC GCAAAATTCT TCTGGACAGC GTCCAGTTAA AAGACCTGTG GAAGAAAATC 
TGCCATCACA GTAGTGGAAT GGAGTTTCAG GATCACCGCT ACTGGCTGAG GACACATCCC 
AACTGCATCG TAGGGAAGGA GTTAGTCAAC TGGCTAATCC GAAATGGGCA CATTGCCACA 
AGGGCACAAG CTGTAGCAAT TGGACAAGCG ATGGTGGACG GACGGTGGCT GGACTGCGTG 
AGCCACCATG ACCAGCTCTT CAGGGATGAG TATGCGCTCT ACAGACCCCT GCAGAGTACA 
GACTTTTCTG AGACCCCTTC TCCGGACAGT GACTCCGTGA ACTCTGTGGA AGGACACTCG 
GAGCCATCAT GGTTTAAAGA CATAAAGTTT GATGACAGCG ACACAGAGCA GATTGCTGAA 
GAAGGTGACG ATAATTTGAC TAACTCTGCC AGTCCTAGCA AGCGCACGTC AGTCAGCAGT 
TTCCAGTCCA CAGTGGACAG TGACTCAGCC GCCTCCATCA GCCTGAACGT GGAGTTGGAC 
AACGTGAACT TCCACATCAA GAAGCCCTCC AAGTACCCAC ACGTGCCCCC TCACCCTGCT 
GACCAAAAAG AGTATTTGAT TTCTGACAAC GGAGGACAAC AACTCTCAAT AAGTGACGCC 
TTCATCAAAG AATCCTTATT TAATCGTCGA GTTGAGGAGA AATCCAAAGA ACTGCCCTTT 
ACGCCTTTGG GCTGGCATCA TAACAATCTG GAGCTGCTGC GGGAGGAGAA TGGAGAGAAG 
CAGGCCATGG AGAGGTTGCT TTCAGCTAAT CATAACCACA TGATGGCACT GCTCCAACAG 
TTGCTCCATA GTGAGTCATT GTCACCATCT TGGAGGGACA TCATTGTATC CCTGGTCTGC 
CAGGTTGTTC AGACAGTCCG ACCTGACGTC AAGAACCGGG ATGACGACAT GGATATCCGT 
CAGTTTGTCC ACGTCAAAAA AATCCCAGGT GGAAAGAAGT TTGATTCTAT GGTTGTCAAT 
GGGTTCGTTT GTACCAAGAA TATTGCACAT AAAAAGATGA ATTCTTGTAT TAAAAACCCC 
AAAATTCTTC TGTTGAAGTG TTCCATTGAG TATCTCTACA GAGAAGAAAC TAAATTTACT 
TGCATTGACC CTATCGTGCT TCAGGAAAGG GAATTCTTGA AGAATTATGT TCAGCGAATA 
GTGGATGTTC GACCCACATT GGTTCTAGTT GAGAAAACGG TGTCTCGGAT TGCCCAGGAC 
CTGTTGCTGG AACACGGCAT CACTTTGGTC ATTAATGTCA AATCACAAGT TTTAGAACGA 
ATCAGTCGGA TGACCCAAGG TGATTTAGTG ATGTCGATGG ACCAGTTGCT CACAAAGCCC 
CACCTGGGCA CCTGTCACAA ATTTTACATG CAGATGTTTC AGCTACCTGG TGAACAAACC 
AAAACTCTGA TGTTTTTTGA AGGCTGTCCA CAGCACCTGG GCTGTACAAT CAAGCTTAGA 
GGAGGCTCCG ATTATGAGCT GGCTCGAGTT AAGGAGATCC TCATATTTAT GATCTGTGTA 
GCATATCATT CTCAACTAGA AATCTCCTTC CTCATGGATG AATTTGCCAT GCCTCCTACA 
TTAACGCAAA ACCCTTCCTT CCACTCTCTG ATTGAGGAAC AGGAGGATGA CGAGGCTGCT 
CAAGAACAGC TCAGCGGAAG TCCCCTCCCC CGGGAACCTG ACTTCCCTCT GGAGTGTCTG 
GCCCCTGATG AGAGCAGTTC ACTGGAATCA AGGACTGTGT TTGGGAAGAG TGACCAGGAA 
CTTAAAGGTG CTCCAAAGGA TGCTGTCTCG TTGAAGCAGC AGGAGTTCGC CACAGCAGCC 
TGCCCGGCCG GCGTCCCCTA TGCTCTCTTT GCATTGGTCC CAGAATCCTT GTTGCCACTG 
CATCTGGGTG ACCAACAGGA TGCCAGGGGC AGCGAGCAGC CAGAGACTGT GCAGCAAACG 
GGTGAGCTAC AGGATCCCAA AAGCCAGATG AGAGCCTTTA GAGACCCTCT GCAGGATGAC 
ACTGGATTGT ATGTCACTGA GGAAGTCACC TCTTCTGAGG ATAAGCGGAA GACTTACTCT 
TTGGCCTTTA AACAGGAATT AAAAGACGTA ATTCTCTGTA TCTCCCCAGT GATCACATTC 
CGAGAGCCTT TCCTTTTAAC CGAGAAGGGG ATGCGATGCT CTCCCCGAGA CTATTTCGCA 
GAGCAGCTTT ACTGGTCTCC TCTCCTCAAT AAAGACTTCA AAGAAATGGA GAGCAGGCGG 
AAGAAGCAGC TGCTCAGGGA TCTCTCCAGC CTCCAGGGCC TGAACGGAAG TGTGCAGGCC 
AAGTCTATCC AAGTGCTGCC CTCACACGAG CTGGTGAGCG CTAGGATTGC TGAGCATCTG 
GGTGACAGCC AGGGCCTGGG CCGGATGCTG GCTGACTACC GGGCCAGAGG AGGGAGAATT 
CAGCAGAATA CTTCAGACCC TTTTGCTCAT TCAAAGGATG CATCAGGTCC TTCAAGTGGC 
AAGTCAGGAA GCAGAACTGA GGGCGATGAA GAGAAAGGGC TGATTCCGAG TGACGCGGTA 
TGGTCAACAA AGGTGGACTG TCTGAACCCT GCCAACCACC AGAGGCTGTG CGTGCTCTTC 
AGCAGCTCTT CTGCCCAGTC CAGCAACGCG CCCAGCGCCT GCGTCAGTCC TTGGATTGTA 
ACAATGGAAT TTTATGGAAA GAATGATCTT ACACTGGGAA TATTTTTAGA GAGATACTGT 
TTCAGGCCTT CTTACCAGTG TCCTAGCATG TTCTGTGATA CCCCCATGGT ACATCACATT 
CGGCGCTTTG TCCATGGCCA AGGCTGTGTG CAGATAATCC TGAAGGAGCT GGATTCTCCA 
GTACCTGGAT ATCAGCATAC AATTCTCACC TATTCCTGGT GCAGAATCTG CAAACAGGTA 
ACACCAGTTG TTGCTCTTTC CAATGAGTCC TGGTCCATGT CATTTGCCAA ATACCTTGAA 
CTTCGGTTTT ATGGGCACCA GTACACCCGT AGAGCCAATG CTGAGCCGTG TGGCCACTCC 
ATCCACCATG ATTATCACCA GTACTTCTCC TATAACCAGA TGGTGGCATC TTTTAGTTAT 
TCTCCCATTC GGCTTCTTGA AGTATGTGTT CCACTCCCCA AAATATTCAT TAAACGTCAG 
GCCCCATTAA AAGTGTCCCT TCTTCAAGAT CTGAAGGACT TCTTTCAAAA AGTTTCACAA 
GTATATCTTG CTGTTGATGA AAGACTTGCA TCTTTGAAAA CTGATACATT TAGCAAAACA 
AGAGAGGAAA AAATGGAAGA TATCTTTGCA CAAAAAGAGA TGGAGGAAGG CGAGTTCAAG 
AACTGGATTG AGAAAATGCA AGCGAGGCTC CTGTCTTCCT CGGTAGACAC CCCTCAGCAG 
CTGCAGTCGG TCCTGGAGTC GCTCATCGCC AAGAAGCAGA GCCTCTGTGA AGTGCTGCAG 
GCCTGGAATA ACAGGTTGCA GGACCTTTTC CAGCAGGAAA AGGGTAGAAA GCGGCCTTCA 
GTTCCTCCAA GTCCTGGAAG ACTGAGACAA GGGGAAGAAA GCAAGATAAG TGCAATGGAT 
GCATCTCCAC GGAATATTTC TCCAGGACTT CAAAATGGAG AAAAAGAGGA CCGCTTCTTG 
ACAACCCTCT CTAGTCAGGG CCCCACCTCT GCTGCTCATC TCCAGCTGCC CAGTCCCCCT 
GAAGTCCTGC CTGAGCAGGC AGGGGGGGCG ACCCCCGAGC CGGATGCAGC CAGCAGTTCT 
GAAGATGTGT TTGATGGACA TTCTCTGGGA TCCACAGACA GCCAGGTGAA GGAAAAATCA 
ACCATGAAGG CCATCTTTGC AAATTTGCTT CCAGGAAATA GCTATAACCC TATTCCGTTT 
CCTTTTGATC CAGATAAACA CTACTTAATG TACGAACACG AACGGGTGCC CATTGCCGTC 
TGTGAGAGGG AGCCCAGCTC CATCATTGCT TTTGCTCTCA GTTGTAAAGA ATACCGAAAT 
GCCTTAGAGG AATTGTCCAA GGCAACTCTG CGGAACAGTG CCGAAGAAGG GCTCCCAGCC 
AACAGTGCTT CAGACAGCAG ACCAAAGGGC GGCAGCCCTG GGAGGCCGCC TGAGCTCGGT 
GGAGGCTGCC CCAACCGGGC GGCCGAGGCA GAGCCGCAGC CAGCCAAAAA GGCTTCTGGG 
ATGTTGTCCT TCTTCCGAGG GACAGCAGGG AAAAGCCCTG ATCTCTCTTC CCAGAAGAGA 
GAGACCTTAC GTGGAGCAGA TAGTGCTTAC TACCAGGTCG GGCAGCCCGG CAAGGAGGGG 
ACGGAGAGTC AAGGCACTGA GCCTCCAGAT GAGATAGATG GAGGAGACGC ACAAAAGAAA 
CAGCTCACAA ATCCTCATGT GGAACTTCAA TTTTCTGACG CTAACGCCAA GTTTTACTGC 
CGGCTCTACT ATGCGGGAGA GTTTCACAAG ATGCGCGAGG TGATCCTGGG CAGCAGCGAG 
GAGGACTTCA TCCGCTCCCT TTCCCACTCG GCACCCTGGC AGGCACGCGG GGGCAAGTCC 
GGAGCGGCCT TCTACGCCAC GGAAGATGAT AGATTCATTT TGAAGCAGAT GCCTCGTCTG 
GAAGTCCAGT CTTTCCTCGA CTTTGCACCA CACTACTTCA ATTATATCAC AAACGCTGTG 
CAACAAAAGA GGCCTACTGC ACTGGCCAAA ATTCTTGGAG TTTACAGAAT TGGTTATAAG 
AATTCTCAGA ACAACACTGA GAAGAAGTTA GATCTCCTTG TCATGGAAAA TCTTTTCTAT 
GGGAGAAAGA TGGCACAGGT TTTTGACTTG AAGGGTTCAC TTAGGAACAG AAATGTAAAA 
ACTGACACCG GGAAAGAGAG CTGTGACGTG GTCCTGCTGG ACGAGAATCT GCTGAAGATG 
GTCCGGGACA GCCCTCTGTA CATCCGCTCG CACTCCAAGG CGGTGCTGCG GGCCTCCATC 
CGCAGCGATG CCCACTTCCT GTCCAGCCAC CTCATCATCG ACTATTCTTT GCTGGTGGGG 
CGAGATGATA CCAGCAATGA GCTTGTCGTT GGGATTATAG ATTATATTCG AACGTTTACA 
TGGGACAAAA AGCTTGAGAT GGTTGTGAAA TCAACAGGAA TTTTAGGAGG ACAAGGTAAA 
ATGCCAACTG TGGTCTCTCC AGAGTTGTAC AGGACCCGAT TTTGTGAAGC GATGGACAAG 
TATTTCCTCA TGGTACCAGA CCACTGGACA GGCTTGGGTC TCAACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_023622526.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023478294.1 Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X10, mRNA.

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CloneID	OHb52475
Clone ID Related Accession (Same CDS sequence)	XM_023622526.1
Accession Version	XM_023622526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGCAGA GAACAGTTTG CAGCACCCCC AGGAGAACAC AGACACAAGA 
AGGAAAACAG AACCTACCTT TGGCGGTCAT GACCCTCGTA CAGCTGTTCA GCTTCGAAGC 
CTCAGCACAG TGTTAAAACG CCTCAAGGAA ATCATGGAGG GGAAAAGCCA GGACAGTGAC 
CTGAAGCAGT ACTGGATGCC GGACAGCCAG TGCAGAGAGT GCTACGACTG CAGCGAGAAG 
TTCACCACCT TCCGGCGCAG GCACCACTGC CGGCTCTGCG GGCAGATTTT CTGCAGCCGC 
TGCTGCAATC AAGAGATCCC TGGGAAGTTT ATGGGCTATA CAGGGGACCT CCGAGCTTGC 
ACATACTGTA GAAAAATAGC CTTAAGTTAT GCTCATTCCA CAGACAGTAA TTCCATTGGG 
GAAGACTTGA ATGCTCTTTC AGATTCTGCT TCCTCTGTGT CTGTACTTGA TCCGAGTGAA 
CCCCGAACAC CTGTTGGGAG TAGGAAAGCC AGCCGGAACA TCTTTTTAGA GGATGATTTT 
GCCTGGCAAA GTTTGATTCA TCCAGACTCC TCAAATACTG CTCTTTCAAC AAGACTTGTA 
TCTGTTCAAG AGGACGCTGG GAAATCTCCT GCTCGAAATA GAATGGACTT GGCAGTGCCT 
GCTCCATTGG ATGCTGTGTG GATTAAATCA TACATACATG GCCCTACTGG GTCAGCCAGC 
ATTACCAACC TCTCACTGGA TCGATCTGGT TCTCCCCTGG TACCTTCATA TGAGACATCT 
GTCAGTCCCC AGGCCAACCG AACATACGTG AGGACAGAGA CCACAGAGGA TGAACGCAAA 
ATTCTTCTGG ACAGCGTCCA GTTAAAAGAC CTGTGGAAGA AAATCTGCCA TCACAGTAGT 
GGAATGGAGT TTCAGGATCA CCGCTACTGG CTGAGGACAC ATCCCAACTG CATCGTAGGG 
AAGGAGTTAG TCAACTGGCT AATCCGAAAT GGGCACATTG CCACAAGGGC ACAAGCTGTA 
GCAATTGGAC AAGCGATGGT GGACGGACGG TGGCTGGACT GCGTGAGCCA CCATGACCAG 
CTCTTCAGGG ATGAGTATGC GCTCTACAGA CCCCTGCAGA GTACAGACTT TTCTGAGACC 
CCTTCTCCGG ACAGTGACTC CGTGAACTCT GTGGAAGGAC ACTCGGAGCC ATCATGGTTT 
AAAGACATAA AGTTTGATGA CAGCGACACA GAGCAGATTG CTGAAGAAGG TGACGATAAT 
TTGACTAACT CTGCCAGTCC TAGCAAGCGC ACGTCAGTCA GCAGTTTCCA GTCCACAGTG 
GACAGTGACT CAGCCGCCTC CATCAGCCTG AACGTGGAGT TGGACAACGT GAACTTCCAC 
ATCAAGAAGC CCTCCAAGTA CCCACACGTG CCCCCTCACC CTGCTGACCA AAAAGAGTAT 
TTGATTTCTG ACAACGGAGG ACAACAACTC TCAATAAGTG ACGCCTTCAT CAAAGAATCC 
TTATTTAATC GTCGAGTTGA GGAGAAATCC AAAGAACTGC CCTTTACGCC TTTGGGCTGG 
CATCATAACA ATCTGGAGCT GCTGCGGGAG GAGAATGGAG AGAAGCAGGC CATGGAGAGG 
TTGCTTTCAG CTAATCATAA CCACATGATG GCACTGCTCC AACAGTTGCT CCATAGTGAG 
TCATTGTCAC CATCTTGGAG GGACATCATT GTATCCCTGG TCTGCCAGGT TGTTCAGACA 
GTCCGACCTG ACGTCAAGAA CCGGGATGAC GACATGGATA TCCGTCAGTT TGTCCACGTC 
AAAAAAATCC CAGGTGGAAA GAAGTTTGAT TCTATGGTTG TCAATGGGTT CGTTTGTACC 
AAGAATATTG CACATAAAAA GATGAATTCT TGTATTAAAA ACCCCAAAAT TCTTCTGTTG 
AAGTGTTCCA TTGAGTATCT CTACAGAGAA GAAACTAAAT TTACTTGCAT TGACCCTATC 
GTGCTTCAGG AAAGGGAATT CTTGAAGAAT TATGTTCAGC GAATAGTGGA TGTTCGACCC 
ACATTGGTTC TAGTTGAGAA AACGGTGTCT CGGATTGCCC AGGACCTGTT GCTGGAACAC 
GGCATCACTT TGGTCATTAA TGTCAAATCA CAAGTTTTAG AACGAATCAG TCGGATGACC 
CAAGGTGATT TAGTGATGTC GATGGACCAG TTGCTCACAA AGCCCCACCT GGGCACCTGT 
CACAAATTTT ACATGCAGAT GTTTCAGCTA CCTGGTGAAC AAACCAAAAC TCTGATGTTT 
TTTGAAGGCT GTCCACAGCA CCTGGGCTGT ACAATCAAGC TTAGAGGAGG CTCCGATTAT 
GAGCTGGCTC GAGTTAAGGA GATCCTCATA TTTATGATCT GTGTAGCATA TCATTCTCAA 
CTAGAAATCT CCTTCCTCAT GGATGAATTT GCCATGCCTC CTACATTAAC GCAAAACCCT 
TCCTTCCACT CTCTGATTGA GGAACAGGAG GATGACGAGG CTGCTCAAGA ACAGCTCAGC 
GGAAGTCCCC TCCCCCGGGA ACCTGACTTC CCTCTGGAGT GTCTGGCCCC TGATGAGAGC 
AGTTCACTGG AATCAAGGAC TGTGTTTGGG AAGAGTGACC AGGAACTTAA AGGTGCTCCA 
AAGGATGCTG TCTCGTTGAA GCAGCAGGAG TTCGCCACAG CAGCCTGCCC GGCCGGCGTC 
CCCTATGCTC TCTTTGCATT GGTCCCAGAA TCCTTGTTGC CACTGCATCT GGGTGACCAA 
CAGGATGCCA GGGGCAGCGA GCAGCCAGAG ACTGTGCAGC AAACGGGTGA GCTACAGGAT 
CCCAAAAGCC AGATGAGAGC CTTTAGAGAC CCTCTGCAGG ATGACACTGG ATTGTATGTC 
ACTGAGGAAG TCACCTCTTC TGAGGATAAG CGGAAGACTT ACTCTTTGGC CTTTAAACAG 
GAATTAAAAG ACGTAATTCT CTGTATCTCC CCAGTGATCA CATTCCGAGA GCCTTTCCTT 
TTAACCGAGA AGGGGATGCG ATGCTCTCCC CGAGACTATT TCGCAGAGCA GCTTTACTGG 
TCTCCTCTCC TCAATAAAGA CTTCAAAGAA ATGGAGAGCA GGCGGAAGAA GCAGCTGCTC 
AGGGATCTCT CCAGCCTCCA GGGCCTGAAC GGAAGTGTGC AGGCCAAGTC TATCCAAGTG 
CTGCCCTCAC ACGAGCTGGT GAGCGCTAGG ATTGCTGAGC ATCTGGGTGA CAGCCAGGGC 
CTGGGCCGGA TGCTGGCTGA CTACCGGGCC AGAGGAGGGA GAATTCAGCA GAATACTTCA 
GACCCTTTTG CTCATTCAAA GGATGCATCA GGTCCTTCAA GTGGCAAGTC AGGAAGCAGA 
ACTGAGGGCG ATGAAGAGAA AGGGCTGATT CCGAGTGACG CGGTATGGTC AACAAAGGTG 
GACTGTCTGA ACCCTGCCAA CCACCAGAGG CTGTGCGTGC TCTTCAGCAG CTCTTCTGCC 
CAGTCCAGCA ACGCGCCCAG CGCCTGCGTC AGTCCTTGGA TTGTAACAAT GGAATTTTAT 
GGAAAGAATG ATCTTACACT GGGAATATTT TTAGAGAGAT ACTGTTTCAG GCCTTCTTAC 
CAGTGTCCTA GCATGTTCTG TGATACCCCC ATGGTACATC ACATTCGGCG CTTTGTCCAT 
GGCCAAGGCT GTGTGCAGAT AATCCTGAAG GAGCTGGATT CTCCAGTACC TGGATATCAG 
CATACAATTC TCACCTATTC CTGGTGCAGA ATCTGCAAAC AGGTAACACC AGTTGTTGCT 
CTTTCCAATG AGTCCTGGTC CATGTCATTT GCCAAATACC TTGAACTTCG GTTTTATGGG 
CACCAGTACA CCCGTAGAGC CAATGCTGAG CCGTGTGGCC ACTCCATCCA CCATGATTAT 
CACCAGTACT TCTCCTATAA CCAGATGGTG GCATCTTTTA GTTATTCTCC CATTCGGCTT 
CTTGAAGTAT GTGTTCCACT CCCCAAAATA TTCATTAAAC GTCAGGCCCC ATTAAAAGTG 
TCCCTTCTTC AAGATCTGAA GGACTTCTTT CAAAAAGTTT CACAAGTATA TCTTGCTGTT 
GATGAAAGAC TTGCATCTTT GAAAACTGAT ACATTTAGCA AAACAAGAGA GGAAAAAATG 
GAAGATATCT TTGCACAAAA AGAGATGGAG GAAGGCGAGT TCAAGAACTG GATTGAGAAA 
ATGCAAGCGA GGCTCCTGTC TTCCTCGGTA GACACCCCTC AGCAGCTGCA GTCGGTCCTG 
GAGTCGCTCA TCGCCAAGAA GCAGAGCCTC TGTGAAGTGC TGCAGGCCTG GAATAACAGG 
TTGCAGGACC TTTTCCAGCA GGAAAAGGGT AGAAAGCGGC CTTCAGTTCC TCCAAGTCCT 
GGAAGACTGA GACAAGGGGA AGAAAGCAAG ATAAGTGCAA TGGATGCATC TCCACGGAAT 
ATTTCTCCAG GACTTCAAAA TGGAGAAAAA GAGGACCGCT TCTTGACAAC CCTCTCTAGT 
CAGGGCCCCA CCTCTGCTGC TCATCTCCAG CTGCCCAGTC CCCCTGAAGT CCTGCCTGAG 
CAGGCAGGGG GGGCGACCCC CGAGCCGGAT GCAGCCAGCA GTTCTGAAGA TGTGTTTGAT 
GGACATTCTC TGGGATCCAC AGACAGCCAG GTGAAGGAAA AATCAACCAT GAAGGCCATC 
TTTGCAAATT TGCTTCCAGG AAATAGCTAT AACCCTATTC CGTTTCCTTT TGATCCAGAT 
AAACACTACT TAATGTACGA ACACGAACGG GTGCCCATTG CCGTCTGTGA GAGGGAGCCC 
AGCTCCATCA TTGCTTTTGC TCTCAGTTGT AAAGAATACC GAAATGCCTT AGAGGAATTG 
TCCAAGGCAA CTCTGCGGAA CAGTGCCGAA GAAGGGCTCC CAGCCAACAG TGCTTCAGAC 
AGCAGACCAA AGGGCGGCAG CCCTGGGAGG CCGCCTGAGC TCGGTGGAGG CTGCCCCAAC 
CGGGCGGCCG AGGCAGAGCC GCAGCCAGAT GAGATAGATG GAGGAGACGC ACAAAAGAAA 
CAGCTCACAA ATCCTCATGT GGAACTTCAA TTTTCTGACG CTAACGCCAA GTTTTACTGC 
CGGCTCTACT ATGCGGGAGA GTTTCACAAG ATGCGCGAGG TGATCCTGGG CAGCAGCGAG 
GAGGACTTCA TCCGCTCCCT TTCCCACTCG GCACCCTGGC AGGCACGCGG GGGCAAGTCC 
GGAGCGGCCT TCTACGCCAC GGAAGATGAT AGATTCATTT TGAAGCAGAT GCCTCGTCTG 
GAAGTCCAGT CTTTCCTCGA CTTTGCACCA CACTACTTCA ATTATATCAC AAACGCTGTG 
CAACAAAAGA GGCCTACTGC ACTGGCCAAA ATTCTTGGAG TTTACAGAAT TGGTTATAAG 
AATTCTCAGA ACAACACTGA GAAGAAGTTA GATCTCCTTG TCATGGAAAA TCTTTTCTAT 
GGGAGAAAGA TGGCACAGGT TTTTGACTTG AAGGGTTCAC TTAGGAACAG AAATGTAAAA 
ACTGACACCG GGAAAGAGAG CTGTGACGTG GTCCTGCTGG ACGAGAATCT GCTGAAGATG 
GTCCGGGACA GCCCTCTGTA CATCCGCTCG CACTCCAAGG CGGTGCTGCG GGCCTCCATC 
CGCAGCGATG CCCACTTCCT GTCCAGCCAC CTCATCATCG ACTATTCTTT GCTGGTGGGG 
CGAGATGATA CCAGCAATGA GCTTGTCGTT GGGATTATAG ATTATATTCG AACGTTTACA 
TGGGACAAAA AGCTTGAGAT GGTTGTGAAA TCAACAGGAA TTTTAGGAGG ACAAGGTAAA 
ATGCCAACTG TGGTCTCTCC AGAGTTGTAC AGGACCCGAT TTTGTGAAGC GATGGACAAG 
TATTTCCTCA TGGTACCAGA CCACTGGACA GGCTTGGGTC TCAACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023478294.1
CDS	114..6344
Translation	MATDDKTSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPAFKSAYSSFVNLFRFNKDRAEGEQQSLTGGWPSPQLPSRTQSVRSPIPYKKQLTEELQRRPSAVLAENSLQHPQENTDTRRKTEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCRECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRMDLAVPAPLDAVWIKSYIHGPTGSASITNLSLDRSGSPLVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAVAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTDFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSPSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHVKKIPGGKKFDSMVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDLLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPGEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEEQEDDEAAQEQLSGSPLPREPDFPLECLAPDESSSLESRTVFGKSDQELKGAPKDAVSLKQQEFATAACPAGVPYALFALVPESLLPLHLGDQQDARGSEQPETVQQTGELQDPKSQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSPRDYFAEQLYWSPLLNKDFKEMESRRKKQLLRDLSSLQGLNGSVQAKSIQVLPSHELVSARIAEHLGDSQGLGRMLADYRARGGRIQQNTSDPFAHSKDASGPSSGKSGSRTEGDEEKGLIPSDAVWSTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLLSSSVDTPQQLQSVLESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQGPTSAAHLQLPSPPEVLPEQAGGATPEPDAASSSEDVFDGHSLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSASDSRPKGGSPGRPPELGGGCPNRAAEAEPQPDEIDGGDAQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSAPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDSPLYIRSHSKAVLRASIRSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_023622526.1
Organism	Equus caballus(horse)
Definition	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023622526.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGCAGA GAACAGTTTG CAGCACCCCC AGGAGAACAC AGACACAAGA 
AGGAAAACAG AACCTACCTT TGGCGGTCAT GACCCTCGTA CAGCTGTTCA GCTTCGAAGC 
CTCAGCACAG TGTTAAAACG CCTCAAGGAA ATCATGGAGG GGAAAAGCCA GGACAGTGAC 
CTGAAGCAGT ACTGGATGCC GGACAGCCAG TGCAGAGAGT GCTACGACTG CAGCGAGAAG 
TTCACCACCT TCCGGCGCAG GCACCACTGC CGGCTCTGCG GGCAGATTTT CTGCAGCCGC 
TGCTGCAATC AAGAGATCCC TGGGAAGTTT ATGGGCTATA CAGGGGACCT CCGAGCTTGC 
ACATACTGTA GAAAAATAGC CTTAAGTTAT GCTCATTCCA CAGACAGTAA TTCCATTGGG 
GAAGACTTGA ATGCTCTTTC AGATTCTGCT TCCTCTGTGT CTGTACTTGA TCCGAGTGAA 
CCCCGAACAC CTGTTGGGAG TAGGAAAGCC AGCCGGAACA TCTTTTTAGA GGATGATTTT 
GCCTGGCAAA GTTTGATTCA TCCAGACTCC TCAAATACTG CTCTTTCAAC AAGACTTGTA 
TCTGTTCAAG AGGACGCTGG GAAATCTCCT GCTCGAAATA GAATGGACTT GGCAGTGCCT 
GCTCCATTGG ATGCTGTGTG GATTAAATCA TACATACATG GCCCTACTGG GTCAGCCAGC 
ATTACCAACC TCTCACTGGA TCGATCTGGT TCTCCCCTGG TACCTTCATA TGAGACATCT 
GTCAGTCCCC AGGCCAACCG AACATACGTG AGGACAGAGA CCACAGAGGA TGAACGCAAA 
ATTCTTCTGG ACAGCGTCCA GTTAAAAGAC CTGTGGAAGA AAATCTGCCA TCACAGTAGT 
GGAATGGAGT TTCAGGATCA CCGCTACTGG CTGAGGACAC ATCCCAACTG CATCGTAGGG 
AAGGAGTTAG TCAACTGGCT AATCCGAAAT GGGCACATTG CCACAAGGGC ACAAGCTGTA 
GCAATTGGAC AAGCGATGGT GGACGGACGG TGGCTGGACT GCGTGAGCCA CCATGACCAG 
CTCTTCAGGG ATGAGTATGC GCTCTACAGA CCCCTGCAGA GTACAGACTT TTCTGAGACC 
CCTTCTCCGG ACAGTGACTC CGTGAACTCT GTGGAAGGAC ACTCGGAGCC ATCATGGTTT 
AAAGACATAA AGTTTGATGA CAGCGACACA GAGCAGATTG CTGAAGAAGG TGACGATAAT 
TTGACTAACT CTGCCAGTCC TAGCAAGCGC ACGTCAGTCA GCAGTTTCCA GTCCACAGTG 
GACAGTGACT CAGCCGCCTC CATCAGCCTG AACGTGGAGT TGGACAACGT GAACTTCCAC 
ATCAAGAAGC CCTCCAAGTA CCCACACGTG CCCCCTCACC CTGCTGACCA AAAAGAGTAT 
TTGATTTCTG ACAACGGAGG ACAACAACTC TCAATAAGTG ACGCCTTCAT CAAAGAATCC 
TTATTTAATC GTCGAGTTGA GGAGAAATCC AAAGAACTGC CCTTTACGCC TTTGGGCTGG 
CATCATAACA ATCTGGAGCT GCTGCGGGAG GAGAATGGAG AGAAGCAGGC CATGGAGAGG 
TTGCTTTCAG CTAATCATAA CCACATGATG GCACTGCTCC AACAGTTGCT CCATAGTGAG 
TCATTGTCAC CATCTTGGAG GGACATCATT GTATCCCTGG TCTGCCAGGT TGTTCAGACA 
GTCCGACCTG ACGTCAAGAA CCGGGATGAC GACATGGATA TCCGTCAGTT TGTCCACGTC 
AAAAAAATCC CAGGTGGAAA GAAGTTTGAT TCTATGGTTG TCAATGGGTT CGTTTGTACC 
AAGAATATTG CACATAAAAA GATGAATTCT TGTATTAAAA ACCCCAAAAT TCTTCTGTTG 
AAGTGTTCCA TTGAGTATCT CTACAGAGAA GAAACTAAAT TTACTTGCAT TGACCCTATC 
GTGCTTCAGG AAAGGGAATT CTTGAAGAAT TATGTTCAGC GAATAGTGGA TGTTCGACCC 
ACATTGGTTC TAGTTGAGAA AACGGTGTCT CGGATTGCCC AGGACCTGTT GCTGGAACAC 
GGCATCACTT TGGTCATTAA TGTCAAATCA CAAGTTTTAG AACGAATCAG TCGGATGACC 
CAAGGTGATT TAGTGATGTC GATGGACCAG TTGCTCACAA AGCCCCACCT GGGCACCTGT 
CACAAATTTT ACATGCAGAT GTTTCAGCTA CCTGGTGAAC AAACCAAAAC TCTGATGTTT 
TTTGAAGGCT GTCCACAGCA CCTGGGCTGT ACAATCAAGC TTAGAGGAGG CTCCGATTAT 
GAGCTGGCTC GAGTTAAGGA GATCCTCATA TTTATGATCT GTGTAGCATA TCATTCTCAA 
CTAGAAATCT CCTTCCTCAT GGATGAATTT GCCATGCCTC CTACATTAAC GCAAAACCCT 
TCCTTCCACT CTCTGATTGA GGAACAGGAG GATGACGAGG CTGCTCAAGA ACAGCTCAGC 
GGAAGTCCCC TCCCCCGGGA ACCTGACTTC CCTCTGGAGT GTCTGGCCCC TGATGAGAGC 
AGTTCACTGG AATCAAGGAC TGTGTTTGGG AAGAGTGACC AGGAACTTAA AGGTGCTCCA 
AAGGATGCTG TCTCGTTGAA GCAGCAGGAG TTCGCCACAG CAGCCTGCCC GGCCGGCGTC 
CCCTATGCTC TCTTTGCATT GGTCCCAGAA TCCTTGTTGC CACTGCATCT GGGTGACCAA 
CAGGATGCCA GGGGCAGCGA GCAGCCAGAG ACTGTGCAGC AAACGGGTGA GCTACAGGAT 
CCCAAAAGCC AGATGAGAGC CTTTAGAGAC CCTCTGCAGG ATGACACTGG ATTGTATGTC 
ACTGAGGAAG TCACCTCTTC TGAGGATAAG CGGAAGACTT ACTCTTTGGC CTTTAAACAG 
GAATTAAAAG ACGTAATTCT CTGTATCTCC CCAGTGATCA CATTCCGAGA GCCTTTCCTT 
TTAACCGAGA AGGGGATGCG ATGCTCTCCC CGAGACTATT TCGCAGAGCA GCTTTACTGG 
TCTCCTCTCC TCAATAAAGA CTTCAAAGAA ATGGAGAGCA GGCGGAAGAA GCAGCTGCTC 
AGGGATCTCT CCAGCCTCCA GGGCCTGAAC GGAAGTGTGC AGGCCAAGTC TATCCAAGTG 
CTGCCCTCAC ACGAGCTGGT GAGCGCTAGG ATTGCTGAGC ATCTGGGTGA CAGCCAGGGC 
CTGGGCCGGA TGCTGGCTGA CTACCGGGCC AGAGGAGGGA GAATTCAGCA GAATACTTCA 
GACCCTTTTG CTCATTCAAA GGATGCATCA GGTCCTTCAA GTGGCAAGTC AGGAAGCAGA 
ACTGAGGGCG ATGAAGAGAA AGGGCTGATT CCGAGTGACG CGGTATGGTC AACAAAGGTG 
GACTGTCTGA ACCCTGCCAA CCACCAGAGG CTGTGCGTGC TCTTCAGCAG CTCTTCTGCC 
CAGTCCAGCA ACGCGCCCAG CGCCTGCGTC AGTCCTTGGA TTGTAACAAT GGAATTTTAT 
GGAAAGAATG ATCTTACACT GGGAATATTT TTAGAGAGAT ACTGTTTCAG GCCTTCTTAC 
CAGTGTCCTA GCATGTTCTG TGATACCCCC ATGGTACATC ACATTCGGCG CTTTGTCCAT 
GGCCAAGGCT GTGTGCAGAT AATCCTGAAG GAGCTGGATT CTCCAGTACC TGGATATCAG 
CATACAATTC TCACCTATTC CTGGTGCAGA ATCTGCAAAC AGGTAACACC AGTTGTTGCT 
CTTTCCAATG AGTCCTGGTC CATGTCATTT GCCAAATACC TTGAACTTCG GTTTTATGGG 
CACCAGTACA CCCGTAGAGC CAATGCTGAG CCGTGTGGCC ACTCCATCCA CCATGATTAT 
CACCAGTACT TCTCCTATAA CCAGATGGTG GCATCTTTTA GTTATTCTCC CATTCGGCTT 
CTTGAAGTAT GTGTTCCACT CCCCAAAATA TTCATTAAAC GTCAGGCCCC ATTAAAAGTG 
TCCCTTCTTC AAGATCTGAA GGACTTCTTT CAAAAAGTTT CACAAGTATA TCTTGCTGTT 
GATGAAAGAC TTGCATCTTT GAAAACTGAT ACATTTAGCA AAACAAGAGA GGAAAAAATG 
GAAGATATCT TTGCACAAAA AGAGATGGAG GAAGGCGAGT TCAAGAACTG GATTGAGAAA 
ATGCAAGCGA GGCTCCTGTC TTCCTCGGTA GACACCCCTC AGCAGCTGCA GTCGGTCCTG 
GAGTCGCTCA TCGCCAAGAA GCAGAGCCTC TGTGAAGTGC TGCAGGCCTG GAATAACAGG 
TTGCAGGACC TTTTCCAGCA GGAAAAGGGT AGAAAGCGGC CTTCAGTTCC TCCAAGTCCT 
GGAAGACTGA GACAAGGGGA AGAAAGCAAG ATAAGTGCAA TGGATGCATC TCCACGGAAT 
ATTTCTCCAG GACTTCAAAA TGGAGAAAAA GAGGACCGCT TCTTGACAAC CCTCTCTAGT 
CAGGGCCCCA CCTCTGCTGC TCATCTCCAG CTGCCCAGTC CCCCTGAAGT CCTGCCTGAG 
CAGGCAGGGG GGGCGACCCC CGAGCCGGAT GCAGCCAGCA GTTCTGAAGA TGTGTTTGAT 
GGACATTCTC TGGGATCCAC AGACAGCCAG GTGAAGGAAA AATCAACCAT GAAGGCCATC 
TTTGCAAATT TGCTTCCAGG AAATAGCTAT AACCCTATTC CGTTTCCTTT TGATCCAGAT 
AAACACTACT TAATGTACGA ACACGAACGG GTGCCCATTG CCGTCTGTGA GAGGGAGCCC 
AGCTCCATCA TTGCTTTTGC TCTCAGTTGT AAAGAATACC GAAATGCCTT AGAGGAATTG 
TCCAAGGCAA CTCTGCGGAA CAGTGCCGAA GAAGGGCTCC CAGCCAACAG TGCTTCAGAC 
AGCAGACCAA AGGGCGGCAG CCCTGGGAGG CCGCCTGAGC TCGGTGGAGG CTGCCCCAAC 
CGGGCGGCCG AGGCAGAGCC GCAGCCAGAT GAGATAGATG GAGGAGACGC ACAAAAGAAA 
CAGCTCACAA ATCCTCATGT GGAACTTCAA TTTTCTGACG CTAACGCCAA GTTTTACTGC 
CGGCTCTACT ATGCGGGAGA GTTTCACAAG ATGCGCGAGG TGATCCTGGG CAGCAGCGAG 
GAGGACTTCA TCCGCTCCCT TTCCCACTCG GCACCCTGGC AGGCACGCGG GGGCAAGTCC 
GGAGCGGCCT TCTACGCCAC GGAAGATGAT AGATTCATTT TGAAGCAGAT GCCTCGTCTG 
GAAGTCCAGT CTTTCCTCGA CTTTGCACCA CACTACTTCA ATTATATCAC AAACGCTGTG 
CAACAAAAGA GGCCTACTGC ACTGGCCAAA ATTCTTGGAG TTTACAGAAT TGGTTATAAG 
AATTCTCAGA ACAACACTGA GAAGAAGTTA GATCTCCTTG TCATGGAAAA TCTTTTCTAT 
GGGAGAAAGA TGGCACAGGT TTTTGACTTG AAGGGTTCAC TTAGGAACAG AAATGTAAAA 
ACTGACACCG GGAAAGAGAG CTGTGACGTG GTCCTGCTGG ACGAGAATCT GCTGAAGATG 
GTCCGGGACA GCCCTCTGTA CATCCGCTCG CACTCCAAGG CGGTGCTGCG GGCCTCCATC 
CGCAGCGATG CCCACTTCCT GTCCAGCCAC CTCATCATCG ACTATTCTTT GCTGGTGGGG 
CGAGATGATA CCAGCAATGA GCTTGTCGTT GGGATTATAG ATTATATTCG AACGTTTACA 
TGGGACAAAA AGCTTGAGAT GGTTGTGAAA TCAACAGGAA TTTTAGGAGG ACAAGGTAAA 
ATGCCAACTG TGGTCTCTCC AGAGTTGTAC AGGACCCGAT TTTGTGAAGC GATGGACAAG 
TATTTCCTCA TGGTACCAGA CCACTGGACA GGCTTGGGTC TCAACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_023622527.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023478295.1 Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X11, mRNA.

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CloneID	OHb52476
Clone ID Related Accession (Same CDS sequence)	XM_023622527.1
Accession Version	XM_023622527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGCAGA GAACAGTTTG CAGCACCCCC AGGAGAACAC AGACACAAGA 
AGGAAAACAG AACCTACCTT TGGCGGTCAT GACCCTCGTA CAGCTGTTCA GCTTCGAAGC 
CTCAGCACAG TGTTAAAACG CCTCAAGGAA ATCATGGAGG GGAAAAGCCA GGACAGTGAC 
CTGAAGCAGT ACTGGATGCC GGACAGCCAG TGCAGAGAGT GCTACGACTG CAGCGAGAAG 
TTCACCACCT TCCGGCGCAG GCACCACTGC CGGCTCTGCG GGCAGATTTT CTGCAGCCGC 
TGCTGCAATC AAGAGATCCC TGGGAAGTTT ATGGGCTATA CAGGGGACCT CCGAGCTTGC 
ACATACTGTA GAAAAATAGC CTTAAGTTAT GCTCATTCCA CAGACAGTAA TTCCATTGGG 
GAAGACTTGA ATGCTCTTTC AGATTCTGCT TCCTCTGTGT CTGTACTTGA TCCGAGTGAA 
CCCCGAACAC CTGTTGGGAG TAGGAAAGCC AGCCGGAACA TCTTTTTAGA GGATGATTTT 
GCCTGGCAAA GTTTGATTCA TCCAGACTCC TCAAATACTG CTCTTTCAAC AAGACTTGTA 
TCTGTTCAAG AGGACGCTGG GAAATCTCCT GCTCGAAATA GAATGGACTT GGCAGTGCCT 
GCTCCATTGG ATGCTGTGTG GATTAAATCA TACATACATG GCCCTACTGG GTCAGCCAGC 
ATTACCAACC TCTCACTGGA TCGATCTGGT TCTCCCCTGG TACCTTCATA TGAGACATCT 
GTCAGTCCCC AGGCCAACCG AACATACGTG AGGACAGAGA CCACAGAGGA TGAACGCAAA 
ATTCTTCTGG ACAGCGTCCA GTTAAAAGAC CTGTGGAAGA AAATCTGCCA TCACAGTAGT 
GGAATGGAGT TTCAGGATCA CCGCTACTGG CTGAGGACAC ATCCCAACTG CATCGTAGGG 
AAGGAGTTAG TCAACTGGCT AATCCGAAAT GGGCACATTG CCACAAGGGC ACAAGCTGTA 
GCAATTGGAC AAGCGATGGT GGACGGACGG TGGCTGGACT GCGTGAGCCA CCATGACCAG 
CTCTTCAGGG ATGAGTATGC GCTCTACAGA CCCCTGCAGA GTACAGACTT TTCTGAGACC 
CCTTCTCCGG ACAGTGACTC CGTGAACTCT GTGGAAGGAC ACTCGGAGCC ATCATGGTTT 
AAAGACATAA AGTTTGATGA CAGCGACACA GAGCAGATTG CTGAAGAAGG TGACGATAAT 
TTGACTAAGT ATTTGATTTC TGACAACGGA GGACAACAAC TCTCAATAAG TGACGCCTTC 
ATCAAAGAAT CCTTATTTAA TCGTCGAGTT GAGGAGAAAT CCAAAGAACT GCCCTTTACG 
CCTTTGGGCT GGCATCATAA CAATCTGGAG CTGCTGCGGG AGGAGAATGG AGAGAAGCAG 
GCCATGGAGA GGTTGCTTTC AGCTAATCAT AACCACATGA TGGCACTGCT CCAACAGTTG 
CTCCATAGTG AGTCATTGTC ACCATCTTGG AGGGACATCA TTGTATCCCT GGTCTGCCAG 
GTTGTTCAGA CAGTCCGACC TGACGTCAAG AACCGGGATG ACGACATGGA TATCCGTCAG 
TTTGTCCACG TCAAAAAAAT CCCAGGTGGA AAGAAGTTTG ATTCTATGGT TGTCAATGGG 
TTCGTTTGTA CCAAGAATAT TGCACATAAA AAGATGAATT CTTGTATTAA AAACCCCAAA 
ATTCTTCTGT TGAAGTGTTC CATTGAGTAT CTCTACAGAG AAGAAACTAA ATTTACTTGC 
ATTGACCCTA TCGTGCTTCA GGAAAGGGAA TTCTTGAAGA ATTATGTTCA GCGAATAGTG 
GATGTTCGAC CCACATTGGT TCTAGTTGAG AAAACGGTGT CTCGGATTGC CCAGGACCTG 
TTGCTGGAAC ACGGCATCAC TTTGGTCATT AATGTCAAAT CACAAGTTTT AGAACGAATC 
AGTCGGATGA CCCAAGGTGA TTTAGTGATG TCGATGGACC AGTTGCTCAC AAAGCCCCAC 
CTGGGCACCT GTCACAAATT TTACATGCAG ATGTTTCAGC TACCTGGTGA ACAAACCAAA 
ACTCTGATGT TTTTTGAAGG CTGTCCACAG CACCTGGGCT GTACAATCAA GCTTAGAGGA 
GGCTCCGATT ATGAGCTGGC TCGAGTTAAG GAGATCCTCA TATTTATGAT CTGTGTAGCA 
TATCATTCTC AACTAGAAAT CTCCTTCCTC ATGGATGAAT TTGCCATGCC TCCTACATTA 
ACGCAAAACC CTTCCTTCCA CTCTCTGATT GAGGAACAGG AGGATGACGA GGCTGCTCAA 
GAACAGCTCA GCGGAAGTCC CCTCCCCCGG GAACCTGACT TCCCTCTGGA GTGTCTGGCC 
CCTGATGAGA GCAGTTCACT GGAATCAAGG ACTGTGTTTG GGAAGAGTGA CCAGGAACTT 
AAAGGTGCTC CAAAGGATGC TGTCTCGTTG AAGCAGCAGG AGTTCGCCAC AGCAGCCTGC 
CCGGCCGGCG TCCCCTATGC TCTCTTTGCA TTGGTCCCAG AATCCTTGTT GCCACTGCAT 
CTGGGTGACC AACAGGATGC CAGGGGCAGC GAGCAGCCAG AGACTGTGCA GCAAACGGGT 
GAGCTACAGG ATCCCAAAAG CCAGATGAGA GCCTTTAGAG ACCCTCTGCA GGATGACACT 
GGATTGTATG TCACTGAGGA AGTCACCTCT TCTGAGGATA AGCGGAAGAC TTACTCTTTG 
GCCTTTAAAC AGGAATTAAA AGACGTAATT CTCTGTATCT CCCCAGTGAT CACATTCCGA 
GAGCCTTTCC TTTTAACCGA GAAGGGGATG CGATGCTCTC CCCGAGACTA TTTCGCAGAG 
CAGCTTTACT GGTCTCCTCT CCTCAATAAA GACTTCAAAG AAATGGAGAG CAGGCGGAAG 
AAGCAGCTGC TCAGGGATCT CTCCAGCCTC CAGGGCCTGA ACGGAAGTGT GCAGGCCAAG 
TCTATCCAAG TGCTGCCCTC ACACGAGCTG GTGAGCGCTA GGATTGCTGA GCATCTGGGT 
GACAGCCAGG GCCTGGGCCG GATGCTGGCT GACTACCGGG CCAGAGGAGG GAGAATTCAG 
CAGAATACTT CAGACCCTTT TGCTCATTCA AAGGATGCAT CAGGTCCTTC AAGTGGCAAG 
TCAGGAAGCA GAACTGAGGG CGATGAAGAG AAAGGGCTGA TTCCGAGTGA CGCGGTATGG 
TCAACAAAGG TGGACTGTCT GAACCCTGCC AACCACCAGA GGCTGTGCGT GCTCTTCAGC 
AGCTCTTCTG CCCAGTCCAG CAACGCGCCC AGCGCCTGCG TCAGTCCTTG GATTGTAACA 
ATGGAATTTT ATGGAAAGAA TGATCTTACA CTGGGAATAT TTTTAGAGAG ATACTGTTTC 
AGGCCTTCTT ACCAGTGTCC TAGCATGTTC TGTGATACCC CCATGGTACA TCACATTCGG 
CGCTTTGTCC ATGGCCAAGG CTGTGTGCAG ATAATCCTGA AGGAGCTGGA TTCTCCAGTA 
CCTGGATATC AGCATACAAT TCTCACCTAT TCCTGGTGCA GAATCTGCAA ACAGGTAACA 
CCAGTTGTTG CTCTTTCCAA TGAGTCCTGG TCCATGTCAT TTGCCAAATA CCTTGAACTT 
CGGTTTTATG GGCACCAGTA CACCCGTAGA GCCAATGCTG AGCCGTGTGG CCACTCCATC 
CACCATGATT ATCACCAGTA CTTCTCCTAT AACCAGATGG TGGCATCTTT TAGTTATTCT 
CCCATTCGGC TTCTTGAAGT ATGTGTTCCA CTCCCCAAAA TATTCATTAA ACGTCAGGCC 
CCATTAAAAG TGTCCCTTCT TCAAGATCTG AAGGACTTCT TTCAAAAAGT TTCACAAGTA 
TATCTTGCTG TTGATGAAAG ACTTGCATCT TTGAAAACTG ATACATTTAG CAAAACAAGA 
GAGGAAAAAA TGGAAGATAT CTTTGCACAA AAAGAGATGG AGGAAGGCGA GTTCAAGAAC 
TGGATTGAGA AAATGCAAGC GAGGCTCCTG TCTTCCTCGG TAGACACCCC TCAGCAGCTG 
CAGTCGGTCC TGGAGTCGCT CATCGCCAAG AAGCAGAGCC TCTGTGAAGT GCTGCAGGCC 
TGGAATAACA GGTTGCAGGA CCTTTTCCAG CAGGAAAAGG GTAGAAAGCG GCCTTCAGTT 
CCTCCAAGTC CTGGAAGACT GAGACAAGGG GAAGAAAGCA AGATAAGTGC AATGGATGCA 
TCTCCACGGA ATATTTCTCC AGGACTTCAA AATGGAGAAA AAGAGGACCG CTTCTTGACA 
ACCCTCTCTA GTCAGGGCCC CACCTCTGCT GCTCATCTCC AGCTGCCCAG TCCCCCTGAA 
GTCCTGCCTG AGCAGGCAGG GGGGGCGACC CCCGAGCCGG ATGCAGCCAG CAGTTCTGAA 
GATGTGTTTG ATGGACATTC TCTGGGATCC ACAGACAGCC AGGTGAAGGA AAAATCAACC 
ATGAAGGCCA TCTTTGCAAA TTTGCTTCCA GGAAATAGCT ATAACCCTAT TCCGTTTCCT 
TTTGATCCAG ATAAACACTA CTTAATGTAC GAACACGAAC GGGTGCCCAT TGCCGTCTGT 
GAGAGGGAGC CCAGCTCCAT CATTGCTTTT GCTCTCAGTT GTAAAGAATA CCGAAATGCC 
TTAGAGGAAT TGTCCAAGGC AACTCTGCGG AACAGTGCCG AAGAAGGGCT CCCAGCCAAC 
AGTGCTTCAG ACAGCAGACC AAAGGGCGGC AGCCCTGGGA GGCCGCCTGA GCTCGGTGGA 
GGCTGCCCCA ACCGGGCGGC CGAGGCAGAG CCGCAGCCAG CCAAAAAGGC TTCTGGGATG 
TTGTCCTTCT TCCGAGGGAC AGCAGGGAAA AGCCCTGATC TCTCTTCCCA GAAGAGAGAG 
ACCTTACGTG GAGCAGATAG TGCTTACTAC CAGGTCGGGC AGCCCGGCAA GGAGGGGACG 
GAGAGTCAAG GCACTGAGCC TCCAGATGAG ATAGATGGAG GAGACGCACA AAAGAAACAG 
CTCACAAATC CTCATGTGGA ACTTCAATTT TCTGACGCTA ACGCCAAGTT TTACTGCCGG 
CTCTACTATG CGGGAGAGTT TCACAAGATG CGCGAGGTGA TCCTGGGCAG CAGCGAGGAG 
GACTTCATCC GCTCCCTTTC CCACTCGGCA CCCTGGCAGG CACGCGGGGG CAAGTCCGGA 
GCGGCCTTCT ACGCCACGGA AGATGATAGA TTCATTTTGA AGCAGATGCC TCGTCTGGAA 
GTCCAGTCTT TCCTCGACTT TGCACCACAC TACTTCAATT ATATCACAAA CGCTGTGCAA 
CAAAAGAGGC CTACTGCACT GGCCAAAATT CTTGGAGTTT ACAGAATTGG TTATAAGAAT 
TCTCAGAACA ACACTGAGAA GAAGTTAGAT CTCCTTGTCA TGGAAAATCT TTTCTATGGG 
AGAAAGATGG CACAGGTTTT TGACTTGAAG GGTTCACTTA GGAACAGAAA TGTAAAAACT 
GACACCGGGA AAGAGAGCTG TGACGTGGTC CTGCTGGACG AGAATCTGCT GAAGATGGTC 
CGGGACAGCC CTCTGTACAT CCGCTCGCAC TCCAAGGCGG TGCTGCGGGC CTCCATCCGC 
AGCGATGCCC ACTTCCTGTC CAGCCACCTC ATCATCGACT ATTCTTTGCT GGTGGGGCGA 
GATGATACCA GCAATGAGCT TGTCGTTGGG ATTATAGATT ATATTCGAAC GTTTACATGG 
GACAAAAAGC TTGAGATGGT TGTGAAATCA ACAGGAATTT TAGGAGGACA AGGTAAAATG 
CCAACTGTGG TCTCTCCAGA GTTGTACAGG ACCCGATTTT GTGAAGCGAT GGACAAGTAT 
TTCCTCATGG TACCAGACCA CTGGACAGGC TTGGGTCTCA ACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023478295.1
CDS	114..6341
Translation	MATDDKTSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPAFKSAYSSFVNLFRFNKDRAEGEQQSLTGGWPSPQLPSRTQSVRSPIPYKKQLTEELQRRPSAVLAENSLQHPQENTDTRRKTEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCRECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRMDLAVPAPLDAVWIKSYIHGPTGSASITNLSLDRSGSPLVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAVAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTDFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTKYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSPSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHVKKIPGGKKFDSMVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDLLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPGEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEEQEDDEAAQEQLSGSPLPREPDFPLECLAPDESSSLESRTVFGKSDQELKGAPKDAVSLKQQEFATAACPAGVPYALFALVPESLLPLHLGDQQDARGSEQPETVQQTGELQDPKSQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSPRDYFAEQLYWSPLLNKDFKEMESRRKKQLLRDLSSLQGLNGSVQAKSIQVLPSHELVSARIAEHLGDSQGLGRMLADYRARGGRIQQNTSDPFAHSKDASGPSSGKSGSRTEGDEEKGLIPSDAVWSTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLLSSSVDTPQQLQSVLESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQGPTSAAHLQLPSPPEVLPEQAGGATPEPDAASSSEDVFDGHSLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSASDSRPKGGSPGRPPELGGGCPNRAAEAEPQPAKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQPGKEGTESQGTEPPDEIDGGDAQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSAPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDSPLYIRSHSKAVLRASIRSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_023622527.1
Organism	Equus caballus(horse)
Definition	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023622527.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGCAGA GAACAGTTTG CAGCACCCCC AGGAGAACAC AGACACAAGA 
AGGAAAACAG AACCTACCTT TGGCGGTCAT GACCCTCGTA CAGCTGTTCA GCTTCGAAGC 
CTCAGCACAG TGTTAAAACG CCTCAAGGAA ATCATGGAGG GGAAAAGCCA GGACAGTGAC 
CTGAAGCAGT ACTGGATGCC GGACAGCCAG TGCAGAGAGT GCTACGACTG CAGCGAGAAG 
TTCACCACCT TCCGGCGCAG GCACCACTGC CGGCTCTGCG GGCAGATTTT CTGCAGCCGC 
TGCTGCAATC AAGAGATCCC TGGGAAGTTT ATGGGCTATA CAGGGGACCT CCGAGCTTGC 
ACATACTGTA GAAAAATAGC CTTAAGTTAT GCTCATTCCA CAGACAGTAA TTCCATTGGG 
GAAGACTTGA ATGCTCTTTC AGATTCTGCT TCCTCTGTGT CTGTACTTGA TCCGAGTGAA 
CCCCGAACAC CTGTTGGGAG TAGGAAAGCC AGCCGGAACA TCTTTTTAGA GGATGATTTT 
GCCTGGCAAA GTTTGATTCA TCCAGACTCC TCAAATACTG CTCTTTCAAC AAGACTTGTA 
TCTGTTCAAG AGGACGCTGG GAAATCTCCT GCTCGAAATA GAATGGACTT GGCAGTGCCT 
GCTCCATTGG ATGCTGTGTG GATTAAATCA TACATACATG GCCCTACTGG GTCAGCCAGC 
ATTACCAACC TCTCACTGGA TCGATCTGGT TCTCCCCTGG TACCTTCATA TGAGACATCT 
GTCAGTCCCC AGGCCAACCG AACATACGTG AGGACAGAGA CCACAGAGGA TGAACGCAAA 
ATTCTTCTGG ACAGCGTCCA GTTAAAAGAC CTGTGGAAGA AAATCTGCCA TCACAGTAGT 
GGAATGGAGT TTCAGGATCA CCGCTACTGG CTGAGGACAC ATCCCAACTG CATCGTAGGG 
AAGGAGTTAG TCAACTGGCT AATCCGAAAT GGGCACATTG CCACAAGGGC ACAAGCTGTA 
GCAATTGGAC AAGCGATGGT GGACGGACGG TGGCTGGACT GCGTGAGCCA CCATGACCAG 
CTCTTCAGGG ATGAGTATGC GCTCTACAGA CCCCTGCAGA GTACAGACTT TTCTGAGACC 
CCTTCTCCGG ACAGTGACTC CGTGAACTCT GTGGAAGGAC ACTCGGAGCC ATCATGGTTT 
AAAGACATAA AGTTTGATGA CAGCGACACA GAGCAGATTG CTGAAGAAGG TGACGATAAT 
TTGACTAAGT ATTTGATTTC TGACAACGGA GGACAACAAC TCTCAATAAG TGACGCCTTC 
ATCAAAGAAT CCTTATTTAA TCGTCGAGTT GAGGAGAAAT CCAAAGAACT GCCCTTTACG 
CCTTTGGGCT GGCATCATAA CAATCTGGAG CTGCTGCGGG AGGAGAATGG AGAGAAGCAG 
GCCATGGAGA GGTTGCTTTC AGCTAATCAT AACCACATGA TGGCACTGCT CCAACAGTTG 
CTCCATAGTG AGTCATTGTC ACCATCTTGG AGGGACATCA TTGTATCCCT GGTCTGCCAG 
GTTGTTCAGA CAGTCCGACC TGACGTCAAG AACCGGGATG ACGACATGGA TATCCGTCAG 
TTTGTCCACG TCAAAAAAAT CCCAGGTGGA AAGAAGTTTG ATTCTATGGT TGTCAATGGG 
TTCGTTTGTA CCAAGAATAT TGCACATAAA AAGATGAATT CTTGTATTAA AAACCCCAAA 
ATTCTTCTGT TGAAGTGTTC CATTGAGTAT CTCTACAGAG AAGAAACTAA ATTTACTTGC 
ATTGACCCTA TCGTGCTTCA GGAAAGGGAA TTCTTGAAGA ATTATGTTCA GCGAATAGTG 
GATGTTCGAC CCACATTGGT TCTAGTTGAG AAAACGGTGT CTCGGATTGC CCAGGACCTG 
TTGCTGGAAC ACGGCATCAC TTTGGTCATT AATGTCAAAT CACAAGTTTT AGAACGAATC 
AGTCGGATGA CCCAAGGTGA TTTAGTGATG TCGATGGACC AGTTGCTCAC AAAGCCCCAC 
CTGGGCACCT GTCACAAATT TTACATGCAG ATGTTTCAGC TACCTGGTGA ACAAACCAAA 
ACTCTGATGT TTTTTGAAGG CTGTCCACAG CACCTGGGCT GTACAATCAA GCTTAGAGGA 
GGCTCCGATT ATGAGCTGGC TCGAGTTAAG GAGATCCTCA TATTTATGAT CTGTGTAGCA 
TATCATTCTC AACTAGAAAT CTCCTTCCTC ATGGATGAAT TTGCCATGCC TCCTACATTA 
ACGCAAAACC CTTCCTTCCA CTCTCTGATT GAGGAACAGG AGGATGACGA GGCTGCTCAA 
GAACAGCTCA GCGGAAGTCC CCTCCCCCGG GAACCTGACT TCCCTCTGGA GTGTCTGGCC 
CCTGATGAGA GCAGTTCACT GGAATCAAGG ACTGTGTTTG GGAAGAGTGA CCAGGAACTT 
AAAGGTGCTC CAAAGGATGC TGTCTCGTTG AAGCAGCAGG AGTTCGCCAC AGCAGCCTGC 
CCGGCCGGCG TCCCCTATGC TCTCTTTGCA TTGGTCCCAG AATCCTTGTT GCCACTGCAT 
CTGGGTGACC AACAGGATGC CAGGGGCAGC GAGCAGCCAG AGACTGTGCA GCAAACGGGT 
GAGCTACAGG ATCCCAAAAG CCAGATGAGA GCCTTTAGAG ACCCTCTGCA GGATGACACT 
GGATTGTATG TCACTGAGGA AGTCACCTCT TCTGAGGATA AGCGGAAGAC TTACTCTTTG 
GCCTTTAAAC AGGAATTAAA AGACGTAATT CTCTGTATCT CCCCAGTGAT CACATTCCGA 
GAGCCTTTCC TTTTAACCGA GAAGGGGATG CGATGCTCTC CCCGAGACTA TTTCGCAGAG 
CAGCTTTACT GGTCTCCTCT CCTCAATAAA GACTTCAAAG AAATGGAGAG CAGGCGGAAG 
AAGCAGCTGC TCAGGGATCT CTCCAGCCTC CAGGGCCTGA ACGGAAGTGT GCAGGCCAAG 
TCTATCCAAG TGCTGCCCTC ACACGAGCTG GTGAGCGCTA GGATTGCTGA GCATCTGGGT 
GACAGCCAGG GCCTGGGCCG GATGCTGGCT GACTACCGGG CCAGAGGAGG GAGAATTCAG 
CAGAATACTT CAGACCCTTT TGCTCATTCA AAGGATGCAT CAGGTCCTTC AAGTGGCAAG 
TCAGGAAGCA GAACTGAGGG CGATGAAGAG AAAGGGCTGA TTCCGAGTGA CGCGGTATGG 
TCAACAAAGG TGGACTGTCT GAACCCTGCC AACCACCAGA GGCTGTGCGT GCTCTTCAGC 
AGCTCTTCTG CCCAGTCCAG CAACGCGCCC AGCGCCTGCG TCAGTCCTTG GATTGTAACA 
ATGGAATTTT ATGGAAAGAA TGATCTTACA CTGGGAATAT TTTTAGAGAG ATACTGTTTC 
AGGCCTTCTT ACCAGTGTCC TAGCATGTTC TGTGATACCC CCATGGTACA TCACATTCGG 
CGCTTTGTCC ATGGCCAAGG CTGTGTGCAG ATAATCCTGA AGGAGCTGGA TTCTCCAGTA 
CCTGGATATC AGCATACAAT TCTCACCTAT TCCTGGTGCA GAATCTGCAA ACAGGTAACA 
CCAGTTGTTG CTCTTTCCAA TGAGTCCTGG TCCATGTCAT TTGCCAAATA CCTTGAACTT 
CGGTTTTATG GGCACCAGTA CACCCGTAGA GCCAATGCTG AGCCGTGTGG CCACTCCATC 
CACCATGATT ATCACCAGTA CTTCTCCTAT AACCAGATGG TGGCATCTTT TAGTTATTCT 
CCCATTCGGC TTCTTGAAGT ATGTGTTCCA CTCCCCAAAA TATTCATTAA ACGTCAGGCC 
CCATTAAAAG TGTCCCTTCT TCAAGATCTG AAGGACTTCT TTCAAAAAGT TTCACAAGTA 
TATCTTGCTG TTGATGAAAG ACTTGCATCT TTGAAAACTG ATACATTTAG CAAAACAAGA 
GAGGAAAAAA TGGAAGATAT CTTTGCACAA AAAGAGATGG AGGAAGGCGA GTTCAAGAAC 
TGGATTGAGA AAATGCAAGC GAGGCTCCTG TCTTCCTCGG TAGACACCCC TCAGCAGCTG 
CAGTCGGTCC TGGAGTCGCT CATCGCCAAG AAGCAGAGCC TCTGTGAAGT GCTGCAGGCC 
TGGAATAACA GGTTGCAGGA CCTTTTCCAG CAGGAAAAGG GTAGAAAGCG GCCTTCAGTT 
CCTCCAAGTC CTGGAAGACT GAGACAAGGG GAAGAAAGCA AGATAAGTGC AATGGATGCA 
TCTCCACGGA ATATTTCTCC AGGACTTCAA AATGGAGAAA AAGAGGACCG CTTCTTGACA 
ACCCTCTCTA GTCAGGGCCC CACCTCTGCT GCTCATCTCC AGCTGCCCAG TCCCCCTGAA 
GTCCTGCCTG AGCAGGCAGG GGGGGCGACC CCCGAGCCGG ATGCAGCCAG CAGTTCTGAA 
GATGTGTTTG ATGGACATTC TCTGGGATCC ACAGACAGCC AGGTGAAGGA AAAATCAACC 
ATGAAGGCCA TCTTTGCAAA TTTGCTTCCA GGAAATAGCT ATAACCCTAT TCCGTTTCCT 
TTTGATCCAG ATAAACACTA CTTAATGTAC GAACACGAAC GGGTGCCCAT TGCCGTCTGT 
GAGAGGGAGC CCAGCTCCAT CATTGCTTTT GCTCTCAGTT GTAAAGAATA CCGAAATGCC 
TTAGAGGAAT TGTCCAAGGC AACTCTGCGG AACAGTGCCG AAGAAGGGCT CCCAGCCAAC 
AGTGCTTCAG ACAGCAGACC AAAGGGCGGC AGCCCTGGGA GGCCGCCTGA GCTCGGTGGA 
GGCTGCCCCA ACCGGGCGGC CGAGGCAGAG CCGCAGCCAG CCAAAAAGGC TTCTGGGATG 
TTGTCCTTCT TCCGAGGGAC AGCAGGGAAA AGCCCTGATC TCTCTTCCCA GAAGAGAGAG 
ACCTTACGTG GAGCAGATAG TGCTTACTAC CAGGTCGGGC AGCCCGGCAA GGAGGGGACG 
GAGAGTCAAG GCACTGAGCC TCCAGATGAG ATAGATGGAG GAGACGCACA AAAGAAACAG 
CTCACAAATC CTCATGTGGA ACTTCAATTT TCTGACGCTA ACGCCAAGTT TTACTGCCGG 
CTCTACTATG CGGGAGAGTT TCACAAGATG CGCGAGGTGA TCCTGGGCAG CAGCGAGGAG 
GACTTCATCC GCTCCCTTTC CCACTCGGCA CCCTGGCAGG CACGCGGGGG CAAGTCCGGA 
GCGGCCTTCT ACGCCACGGA AGATGATAGA TTCATTTTGA AGCAGATGCC TCGTCTGGAA 
GTCCAGTCTT TCCTCGACTT TGCACCACAC TACTTCAATT ATATCACAAA CGCTGTGCAA 
CAAAAGAGGC CTACTGCACT GGCCAAAATT CTTGGAGTTT ACAGAATTGG TTATAAGAAT 
TCTCAGAACA ACACTGAGAA GAAGTTAGAT CTCCTTGTCA TGGAAAATCT TTTCTATGGG 
AGAAAGATGG CACAGGTTTT TGACTTGAAG GGTTCACTTA GGAACAGAAA TGTAAAAACT 
GACACCGGGA AAGAGAGCTG TGACGTGGTC CTGCTGGACG AGAATCTGCT GAAGATGGTC 
CGGGACAGCC CTCTGTACAT CCGCTCGCAC TCCAAGGCGG TGCTGCGGGC CTCCATCCGC 
AGCGATGCCC ACTTCCTGTC CAGCCACCTC ATCATCGACT ATTCTTTGCT GGTGGGGCGA 
GATGATACCA GCAATGAGCT TGTCGTTGGG ATTATAGATT ATATTCGAAC GTTTACATGG 
GACAAAAAGC TTGAGATGGT TGTGAAATCA ACAGGAATTT TAGGAGGACA AGGTAAAATG 
CCAACTGTGG TCTCTCCAGA GTTGTACAGG ACCCGATTTT GTGAAGCGAT GGACAAGTAT 
TTCCTCATGG TACCAGACCA CTGGACAGGC TTGGGTCTCA ACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_023622528.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023478296.1 Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X12, mRNA.

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CloneID	OHb52477
Clone ID Related Accession (Same CDS sequence)	XM_023622528.1
Accession Version	XM_023622528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGCAGA GAACAGTTTG CAGCACCCCC AGGAGAACAC AGACACAAGA 
AGGAAAACAG AACCTACCTT TGGCGGTCAT GACCCTCGTA CAGCTGTTCA GCTTCGAAGC 
CTCAGCACAG TGTTAAAACG CCTCAAGGAA ATCATGGAGG GGAAAAGCCA GGACAGTGAC 
CTGAAGCAGT ACTGGATGCC GGACAGCCAG TGCAGAGAGT GCTACGACTG CAGCGAGAAG 
TTCACCACCT TCCGGCGCAG GCACCACTGC CGGCTCTGCG GGCAGATTTT CTGCAGCCGC 
TGCTGCAATC AAGAGATCCC TGGGAAGTTT ATGGGCTATA CAGGGGACCT CCGAGCTTGC 
ACATACTGTA GAAAAATAGC CTTAAGTTAT GCTCATTCCA CAGACAGTAA TTCCATTGGG 
GAAGACTTGA ATGCTCTTTC AGATTCTGCT TCCTCTGTGT CTGTACTTGA TCCGAGTGAA 
CCCCGAACAC CTGTTGGGAG TAGGAAAGCC AGCCGGAACA TCTTTTTAGA GGATGATTTT 
GCCTGGCAAA GTTTGATTCA TCCAGACTCC TCAAATACTG CTCTTTCAAC AAGACTTGTA 
TCTGTTCAAG AGGACGCTGG GAAATCTCCT GCTCGAAATA GAATGGACTT GGCAGTGCCT 
GCTCCATTGG ATGCTGTGTG GATTAAATCA TACATACATG GCCCTACTGG GTCAGCCAGC 
ATTACCAACC TCTCACTGGA TCGATCTGGT TCTCCCCTGG TACCTTCATA TGAGACATCT 
GTCAGTCCCC AGGCCAACCG AACATACGTG AGGACAGAGA CCACAGAGGA TGAACGCAAA 
ATTCTTCTGG ACAGCGTCCA GTTAAAAGAC CTGTGGAAGA AAATCTGCCA TCACAGTAGT 
GGAATGGAGT TTCAGGATCA CCGCTACTGG CTGAGGACAC ATCCCAACTG CATCGTAGGG 
AAGGAGTTAG TCAACTGGCT AATCCGAAAT GGGCACATTG CCACAAGGGC ACAAGCTGTA 
GCAATTGGAC AAGCGATGGT GGACGGACGG TGGCTGGACT GCGTGAGCCA CCATGACCAG 
CTCTTCAGGG ATGAGTATGC GCTCTACAGA CCCCTGCAGA GTACAGACTT TTCTGAGACC 
CCTTCTCCGG ACAGTGACTC CGTGAACTCT GTGGAAGGAC ACTCGGAGCC ATCATGGTTT 
AAAGACATAA AGTTTGATGA CAGCGACACA GAGCAGATTG CTGAAGAAGA GTATTTGATT 
TCTGACAACG GAGGACAACA ACTCTCAATA AGTGACGCCT TCATCAAAGA ATCCTTATTT 
AATCGTCGAG TTGAGGAGAA ATCCAAAGAA CTGCCCTTTA CGCCTTTGGG CTGGCATCAT 
AACAATCTGG AGCTGCTGCG GGAGGAGAAT GGAGAGAAGC AGGCCATGGA GAGGTTGCTT 
TCAGCTAATC ATAACCACAT GATGGCACTG CTCCAACAGT TGCTCCATAG TGAGTCATTG 
TCACCATCTT GGAGGGACAT CATTGTATCC CTGGTCTGCC AGGTTGTTCA GACAGTCCGA 
CCTGACGTCA AGAACCGGGA TGACGACATG GATATCCGTC AGTTTGTCCA CGTCAAAAAA 
ATCCCAGGTG GAAAGAAGTT TGATTCTATG GTTGTCAATG GGTTCGTTTG TACCAAGAAT 
ATTGCACATA AAAAGATGAA TTCTTGTATT AAAAACCCCA AAATTCTTCT GTTGAAGTGT 
TCCATTGAGT ATCTCTACAG AGAAGAAACT AAATTTACTT GCATTGACCC TATCGTGCTT 
CAGGAAAGGG AATTCTTGAA GAATTATGTT CAGCGAATAG TGGATGTTCG ACCCACATTG 
GTTCTAGTTG AGAAAACGGT GTCTCGGATT GCCCAGGACC TGTTGCTGGA ACACGGCATC 
ACTTTGGTCA TTAATGTCAA ATCACAAGTT TTAGAACGAA TCAGTCGGAT GACCCAAGGT 
GATTTAGTGA TGTCGATGGA CCAGTTGCTC ACAAAGCCCC ACCTGGGCAC CTGTCACAAA 
TTTTACATGC AGATGTTTCA GCTACCTGGT GAACAAACCA AAACTCTGAT GTTTTTTGAA 
GGCTGTCCAC AGCACCTGGG CTGTACAATC AAGCTTAGAG GAGGCTCCGA TTATGAGCTG 
GCTCGAGTTA AGGAGATCCT CATATTTATG ATCTGTGTAG CATATCATTC TCAACTAGAA 
ATCTCCTTCC TCATGGATGA ATTTGCCATG CCTCCTACAT TAACGCAAAA CCCTTCCTTC 
CACTCTCTGA TTGAGGAACA GGAGGATGAC GAGGCTGCTC AAGAACAGCT CAGCGGAAGT 
CCCCTCCCCC GGGAACCTGA CTTCCCTCTG GAGTGTCTGG CCCCTGATGA GAGCAGTTCA 
CTGGAATCAA GGACTGTGTT TGGGAAGAGT GACCAGGAAC TTAAAGGTGC TCCAAAGGAT 
GCTGTCTCGT TGAAGCAGCA GGAGTTCGCC ACAGCAGCCT GCCCGGCCGG CGTCCCCTAT 
GCTCTCTTTG CATTGGTCCC AGAATCCTTG TTGCCACTGC ATCTGGGTGA CCAACAGGAT 
GCCAGGGGCA GCGAGCAGCC AGAGACTGTG CAGCAAACGG GTGAGCTACA GGATCCCAAA 
AGCCAGATGA GAGCCTTTAG AGACCCTCTG CAGGATGACA CTGGATTGTA TGTCACTGAG 
GAAGTCACCT CTTCTGAGGA TAAGCGGAAG ACTTACTCTT TGGCCTTTAA ACAGGAATTA 
AAAGACGTAA TTCTCTGTAT CTCCCCAGTG ATCACATTCC GAGAGCCTTT CCTTTTAACC 
GAGAAGGGGA TGCGATGCTC TCCCCGAGAC TATTTCGCAG AGCAGCTTTA CTGGTCTCCT 
CTCCTCAATA AAGACTTCAA AGAAATGGAG AGCAGGCGGA AGAAGCAGCT GCTCAGGGAT 
CTCTCCAGCC TCCAGGGCCT GAACGGAAGT GTGCAGGCCA AGTCTATCCA AGTGCTGCCC 
TCACACGAGC TGGTGAGCGC TAGGATTGCT GAGCATCTGG GTGACAGCCA GGGCCTGGGC 
CGGATGCTGG CTGACTACCG GGCCAGAGGA GGGAGAATTC AGCAGAATAC TTCAGACCCT 
TTTGCTCATT CAAAGGATGC ATCAGGTCCT TCAAGTGGCA AGTCAGGAAG CAGAACTGAG 
GGCGATGAAG AGAAAGGGCT GATTCCGAGT GACGCGGTAT GGTCAACAAA GGTGGACTGT 
CTGAACCCTG CCAACCACCA GAGGCTGTGC GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC 
AGCAACGCGC CCAGCGCCTG CGTCAGTCCT TGGATTGTAA CAATGGAATT TTATGGAAAG 
AATGATCTTA CACTGGGAAT ATTTTTAGAG AGATACTGTT TCAGGCCTTC TTACCAGTGT 
CCTAGCATGT TCTGTGATAC CCCCATGGTA CATCACATTC GGCGCTTTGT CCATGGCCAA 
GGCTGTGTGC AGATAATCCT GAAGGAGCTG GATTCTCCAG TACCTGGATA TCAGCATACA 
ATTCTCACCT ATTCCTGGTG CAGAATCTGC AAACAGGTAA CACCAGTTGT TGCTCTTTCC 
AATGAGTCCT GGTCCATGTC ATTTGCCAAA TACCTTGAAC TTCGGTTTTA TGGGCACCAG 
TACACCCGTA GAGCCAATGC TGAGCCGTGT GGCCACTCCA TCCACCATGA TTATCACCAG 
TACTTCTCCT ATAACCAGAT GGTGGCATCT TTTAGTTATT CTCCCATTCG GCTTCTTGAA 
GTATGTGTTC CACTCCCCAA AATATTCATT AAACGTCAGG CCCCATTAAA AGTGTCCCTT 
CTTCAAGATC TGAAGGACTT CTTTCAAAAA GTTTCACAAG TATATCTTGC TGTTGATGAA 
AGACTTGCAT CTTTGAAAAC TGATACATTT AGCAAAACAA GAGAGGAAAA AATGGAAGAT 
ATCTTTGCAC AAAAAGAGAT GGAGGAAGGC GAGTTCAAGA ACTGGATTGA GAAAATGCAA 
GCGAGGCTCC TGTCTTCCTC GGTAGACACC CCTCAGCAGC TGCAGTCGGT CCTGGAGTCG 
CTCATCGCCA AGAAGCAGAG CCTCTGTGAA GTGCTGCAGG CCTGGAATAA CAGGTTGCAG 
GACCTTTTCC AGCAGGAAAA GGGTAGAAAG CGGCCTTCAG TTCCTCCAAG TCCTGGAAGA 
CTGAGACAAG GGGAAGAAAG CAAGATAAGT GCAATGGATG CATCTCCACG GAATATTTCT 
CCAGGACTTC AAAATGGAGA AAAAGAGGAC CGCTTCTTGA CAACCCTCTC TAGTCAGGGC 
CCCACCTCTG CTGCTCATCT CCAGCTGCCC AGTCCCCCTG AAGTCCTGCC TGAGCAGGCA 
GGGGGGGCGA CCCCCGAGCC GGATGCAGCC AGCAGTTCTG AAGATGTGTT TGATGGACAT 
TCTCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAATCAA CCATGAAGGC CATCTTTGCA 
AATTTGCTTC CAGGAAATAG CTATAACCCT ATTCCGTTTC CTTTTGATCC AGATAAACAC 
TACTTAATGT ACGAACACGA ACGGGTGCCC ATTGCCGTCT GTGAGAGGGA GCCCAGCTCC 
ATCATTGCTT TTGCTCTCAG TTGTAAAGAA TACCGAAATG CCTTAGAGGA ATTGTCCAAG 
GCAACTCTGC GGAACAGTGC CGAAGAAGGG CTCCCAGCCA ACAGTGCTTC AGACAGCAGA 
CCAAAGGGCG GCAGCCCTGG GAGGCCGCCT GAGCTCGGTG GAGGCTGCCC CAACCGGGCG 
GCCGAGGCAG AGCCGCAGCC AGCCAAAAAG GCTTCTGGGA TGTTGTCCTT CTTCCGAGGG 
ACAGCAGGGA AAAGCCCTGA TCTCTCTTCC CAGAAGAGAG AGACCTTACG TGGAGCAGAT 
AGTGCTTACT ACCAGGTCGG GCAGCCCGGC AAGGAGGGGA CGGAGAGTCA AGGCACTGAG 
CCTCCAGATG AGATAGATGG AGGAGACGCA CAAAAGAAAC AGCTCACAAA TCCTCATGTG 
GAACTTCAAT TTTCTGACGC TAACGCCAAG TTTTACTGCC GGCTCTACTA TGCGGGAGAG 
TTTCACAAGA TGCGCGAGGT GATCCTGGGC AGCAGCGAGG AGGACTTCAT CCGCTCCCTT 
TCCCACTCGG CACCCTGGCA GGCACGCGGG GGCAAGTCCG GAGCGGCCTT CTACGCCACG 
GAAGATGATA GATTCATTTT GAAGCAGATG CCTCGTCTGG AAGTCCAGTC TTTCCTCGAC 
TTTGCACCAC ACTACTTCAA TTATATCACA AACGCTGTGC AACAAAAGAG GCCTACTGCA 
CTGGCCAAAA TTCTTGGAGT TTACAGAATT GGTTATAAGA ATTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGACTTGA AGGGTTCACT TAGGAACAGA AATGTAAAAA CTGACACCGG GAAAGAGAGC 
TGTGACGTGG TCCTGCTGGA CGAGAATCTG CTGAAGATGG TCCGGGACAG CCCTCTGTAC 
ATCCGCTCGC ACTCCAAGGC GGTGCTGCGG GCCTCCATCC GCAGCGATGC CCACTTCCTG 
TCCAGCCACC TCATCATCGA CTATTCTTTG CTGGTGGGGC GAGATGATAC CAGCAATGAG 
CTTGTCGTTG GGATTATAGA TTATATTCGA ACGTTTACAT GGGACAAAAA GCTTGAGATG 
GTTGTGAAAT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACTGT GGTCTCTCCA 
GAGTTGTACA GGACCCGATT TTGTGAAGCG ATGGACAAGT ATTTCCTCAT GGTACCAGAC 
CACTGGACAG GCTTGGGTCT CAACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023478296.1
CDS	114..6323
Translation	MATDDKTSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPAFKSAYSSFVNLFRFNKDRAEGEQQSLTGGWPSPQLPSRTQSVRSPIPYKKQLTEELQRRPSAVLAENSLQHPQENTDTRRKTEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCRECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRMDLAVPAPLDAVWIKSYIHGPTGSASITNLSLDRSGSPLVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAVAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTDFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSPSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHVKKIPGGKKFDSMVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDLLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPGEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEEQEDDEAAQEQLSGSPLPREPDFPLECLAPDESSSLESRTVFGKSDQELKGAPKDAVSLKQQEFATAACPAGVPYALFALVPESLLPLHLGDQQDARGSEQPETVQQTGELQDPKSQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSPRDYFAEQLYWSPLLNKDFKEMESRRKKQLLRDLSSLQGLNGSVQAKSIQVLPSHELVSARIAEHLGDSQGLGRMLADYRARGGRIQQNTSDPFAHSKDASGPSSGKSGSRTEGDEEKGLIPSDAVWSTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLLSSSVDTPQQLQSVLESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQGPTSAAHLQLPSPPEVLPEQAGGATPEPDAASSSEDVFDGHSLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSASDSRPKGGSPGRPPELGGGCPNRAAEAEPQPAKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQPGKEGTESQGTEPPDEIDGGDAQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSAPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDSPLYIRSHSKAVLRASIRSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_023622528.1
Organism	Equus caballus(horse)
Definition	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023622528.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGCAGA GAACAGTTTG CAGCACCCCC AGGAGAACAC AGACACAAGA 
AGGAAAACAG AACCTACCTT TGGCGGTCAT GACCCTCGTA CAGCTGTTCA GCTTCGAAGC 
CTCAGCACAG TGTTAAAACG CCTCAAGGAA ATCATGGAGG GGAAAAGCCA GGACAGTGAC 
CTGAAGCAGT ACTGGATGCC GGACAGCCAG TGCAGAGAGT GCTACGACTG CAGCGAGAAG 
TTCACCACCT TCCGGCGCAG GCACCACTGC CGGCTCTGCG GGCAGATTTT CTGCAGCCGC 
TGCTGCAATC AAGAGATCCC TGGGAAGTTT ATGGGCTATA CAGGGGACCT CCGAGCTTGC 
ACATACTGTA GAAAAATAGC CTTAAGTTAT GCTCATTCCA CAGACAGTAA TTCCATTGGG 
GAAGACTTGA ATGCTCTTTC AGATTCTGCT TCCTCTGTGT CTGTACTTGA TCCGAGTGAA 
CCCCGAACAC CTGTTGGGAG TAGGAAAGCC AGCCGGAACA TCTTTTTAGA GGATGATTTT 
GCCTGGCAAA GTTTGATTCA TCCAGACTCC TCAAATACTG CTCTTTCAAC AAGACTTGTA 
TCTGTTCAAG AGGACGCTGG GAAATCTCCT GCTCGAAATA GAATGGACTT GGCAGTGCCT 
GCTCCATTGG ATGCTGTGTG GATTAAATCA TACATACATG GCCCTACTGG GTCAGCCAGC 
ATTACCAACC TCTCACTGGA TCGATCTGGT TCTCCCCTGG TACCTTCATA TGAGACATCT 
GTCAGTCCCC AGGCCAACCG AACATACGTG AGGACAGAGA CCACAGAGGA TGAACGCAAA 
ATTCTTCTGG ACAGCGTCCA GTTAAAAGAC CTGTGGAAGA AAATCTGCCA TCACAGTAGT 
GGAATGGAGT TTCAGGATCA CCGCTACTGG CTGAGGACAC ATCCCAACTG CATCGTAGGG 
AAGGAGTTAG TCAACTGGCT AATCCGAAAT GGGCACATTG CCACAAGGGC ACAAGCTGTA 
GCAATTGGAC AAGCGATGGT GGACGGACGG TGGCTGGACT GCGTGAGCCA CCATGACCAG 
CTCTTCAGGG ATGAGTATGC GCTCTACAGA CCCCTGCAGA GTACAGACTT TTCTGAGACC 
CCTTCTCCGG ACAGTGACTC CGTGAACTCT GTGGAAGGAC ACTCGGAGCC ATCATGGTTT 
AAAGACATAA AGTTTGATGA CAGCGACACA GAGCAGATTG CTGAAGAAGA GTATTTGATT 
TCTGACAACG GAGGACAACA ACTCTCAATA AGTGACGCCT TCATCAAAGA ATCCTTATTT 
AATCGTCGAG TTGAGGAGAA ATCCAAAGAA CTGCCCTTTA CGCCTTTGGG CTGGCATCAT 
AACAATCTGG AGCTGCTGCG GGAGGAGAAT GGAGAGAAGC AGGCCATGGA GAGGTTGCTT 
TCAGCTAATC ATAACCACAT GATGGCACTG CTCCAACAGT TGCTCCATAG TGAGTCATTG 
TCACCATCTT GGAGGGACAT CATTGTATCC CTGGTCTGCC AGGTTGTTCA GACAGTCCGA 
CCTGACGTCA AGAACCGGGA TGACGACATG GATATCCGTC AGTTTGTCCA CGTCAAAAAA 
ATCCCAGGTG GAAAGAAGTT TGATTCTATG GTTGTCAATG GGTTCGTTTG TACCAAGAAT 
ATTGCACATA AAAAGATGAA TTCTTGTATT AAAAACCCCA AAATTCTTCT GTTGAAGTGT 
TCCATTGAGT ATCTCTACAG AGAAGAAACT AAATTTACTT GCATTGACCC TATCGTGCTT 
CAGGAAAGGG AATTCTTGAA GAATTATGTT CAGCGAATAG TGGATGTTCG ACCCACATTG 
GTTCTAGTTG AGAAAACGGT GTCTCGGATT GCCCAGGACC TGTTGCTGGA ACACGGCATC 
ACTTTGGTCA TTAATGTCAA ATCACAAGTT TTAGAACGAA TCAGTCGGAT GACCCAAGGT 
GATTTAGTGA TGTCGATGGA CCAGTTGCTC ACAAAGCCCC ACCTGGGCAC CTGTCACAAA 
TTTTACATGC AGATGTTTCA GCTACCTGGT GAACAAACCA AAACTCTGAT GTTTTTTGAA 
GGCTGTCCAC AGCACCTGGG CTGTACAATC AAGCTTAGAG GAGGCTCCGA TTATGAGCTG 
GCTCGAGTTA AGGAGATCCT CATATTTATG ATCTGTGTAG CATATCATTC TCAACTAGAA 
ATCTCCTTCC TCATGGATGA ATTTGCCATG CCTCCTACAT TAACGCAAAA CCCTTCCTTC 
CACTCTCTGA TTGAGGAACA GGAGGATGAC GAGGCTGCTC AAGAACAGCT CAGCGGAAGT 
CCCCTCCCCC GGGAACCTGA CTTCCCTCTG GAGTGTCTGG CCCCTGATGA GAGCAGTTCA 
CTGGAATCAA GGACTGTGTT TGGGAAGAGT GACCAGGAAC TTAAAGGTGC TCCAAAGGAT 
GCTGTCTCGT TGAAGCAGCA GGAGTTCGCC ACAGCAGCCT GCCCGGCCGG CGTCCCCTAT 
GCTCTCTTTG CATTGGTCCC AGAATCCTTG TTGCCACTGC ATCTGGGTGA CCAACAGGAT 
GCCAGGGGCA GCGAGCAGCC AGAGACTGTG CAGCAAACGG GTGAGCTACA GGATCCCAAA 
AGCCAGATGA GAGCCTTTAG AGACCCTCTG CAGGATGACA CTGGATTGTA TGTCACTGAG 
GAAGTCACCT CTTCTGAGGA TAAGCGGAAG ACTTACTCTT TGGCCTTTAA ACAGGAATTA 
AAAGACGTAA TTCTCTGTAT CTCCCCAGTG ATCACATTCC GAGAGCCTTT CCTTTTAACC 
GAGAAGGGGA TGCGATGCTC TCCCCGAGAC TATTTCGCAG AGCAGCTTTA CTGGTCTCCT 
CTCCTCAATA AAGACTTCAA AGAAATGGAG AGCAGGCGGA AGAAGCAGCT GCTCAGGGAT 
CTCTCCAGCC TCCAGGGCCT GAACGGAAGT GTGCAGGCCA AGTCTATCCA AGTGCTGCCC 
TCACACGAGC TGGTGAGCGC TAGGATTGCT GAGCATCTGG GTGACAGCCA GGGCCTGGGC 
CGGATGCTGG CTGACTACCG GGCCAGAGGA GGGAGAATTC AGCAGAATAC TTCAGACCCT 
TTTGCTCATT CAAAGGATGC ATCAGGTCCT TCAAGTGGCA AGTCAGGAAG CAGAACTGAG 
GGCGATGAAG AGAAAGGGCT GATTCCGAGT GACGCGGTAT GGTCAACAAA GGTGGACTGT 
CTGAACCCTG CCAACCACCA GAGGCTGTGC GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC 
AGCAACGCGC CCAGCGCCTG CGTCAGTCCT TGGATTGTAA CAATGGAATT TTATGGAAAG 
AATGATCTTA CACTGGGAAT ATTTTTAGAG AGATACTGTT TCAGGCCTTC TTACCAGTGT 
CCTAGCATGT TCTGTGATAC CCCCATGGTA CATCACATTC GGCGCTTTGT CCATGGCCAA 
GGCTGTGTGC AGATAATCCT GAAGGAGCTG GATTCTCCAG TACCTGGATA TCAGCATACA 
ATTCTCACCT ATTCCTGGTG CAGAATCTGC AAACAGGTAA CACCAGTTGT TGCTCTTTCC 
AATGAGTCCT GGTCCATGTC ATTTGCCAAA TACCTTGAAC TTCGGTTTTA TGGGCACCAG 
TACACCCGTA GAGCCAATGC TGAGCCGTGT GGCCACTCCA TCCACCATGA TTATCACCAG 
TACTTCTCCT ATAACCAGAT GGTGGCATCT TTTAGTTATT CTCCCATTCG GCTTCTTGAA 
GTATGTGTTC CACTCCCCAA AATATTCATT AAACGTCAGG CCCCATTAAA AGTGTCCCTT 
CTTCAAGATC TGAAGGACTT CTTTCAAAAA GTTTCACAAG TATATCTTGC TGTTGATGAA 
AGACTTGCAT CTTTGAAAAC TGATACATTT AGCAAAACAA GAGAGGAAAA AATGGAAGAT 
ATCTTTGCAC AAAAAGAGAT GGAGGAAGGC GAGTTCAAGA ACTGGATTGA GAAAATGCAA 
GCGAGGCTCC TGTCTTCCTC GGTAGACACC CCTCAGCAGC TGCAGTCGGT CCTGGAGTCG 
CTCATCGCCA AGAAGCAGAG CCTCTGTGAA GTGCTGCAGG CCTGGAATAA CAGGTTGCAG 
GACCTTTTCC AGCAGGAAAA GGGTAGAAAG CGGCCTTCAG TTCCTCCAAG TCCTGGAAGA 
CTGAGACAAG GGGAAGAAAG CAAGATAAGT GCAATGGATG CATCTCCACG GAATATTTCT 
CCAGGACTTC AAAATGGAGA AAAAGAGGAC CGCTTCTTGA CAACCCTCTC TAGTCAGGGC 
CCCACCTCTG CTGCTCATCT CCAGCTGCCC AGTCCCCCTG AAGTCCTGCC TGAGCAGGCA 
GGGGGGGCGA CCCCCGAGCC GGATGCAGCC AGCAGTTCTG AAGATGTGTT TGATGGACAT 
TCTCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAATCAA CCATGAAGGC CATCTTTGCA 
AATTTGCTTC CAGGAAATAG CTATAACCCT ATTCCGTTTC CTTTTGATCC AGATAAACAC 
TACTTAATGT ACGAACACGA ACGGGTGCCC ATTGCCGTCT GTGAGAGGGA GCCCAGCTCC 
ATCATTGCTT TTGCTCTCAG TTGTAAAGAA TACCGAAATG CCTTAGAGGA ATTGTCCAAG 
GCAACTCTGC GGAACAGTGC CGAAGAAGGG CTCCCAGCCA ACAGTGCTTC AGACAGCAGA 
CCAAAGGGCG GCAGCCCTGG GAGGCCGCCT GAGCTCGGTG GAGGCTGCCC CAACCGGGCG 
GCCGAGGCAG AGCCGCAGCC AGCCAAAAAG GCTTCTGGGA TGTTGTCCTT CTTCCGAGGG 
ACAGCAGGGA AAAGCCCTGA TCTCTCTTCC CAGAAGAGAG AGACCTTACG TGGAGCAGAT 
AGTGCTTACT ACCAGGTCGG GCAGCCCGGC AAGGAGGGGA CGGAGAGTCA AGGCACTGAG 
CCTCCAGATG AGATAGATGG AGGAGACGCA CAAAAGAAAC AGCTCACAAA TCCTCATGTG 
GAACTTCAAT TTTCTGACGC TAACGCCAAG TTTTACTGCC GGCTCTACTA TGCGGGAGAG 
TTTCACAAGA TGCGCGAGGT GATCCTGGGC AGCAGCGAGG AGGACTTCAT CCGCTCCCTT 
TCCCACTCGG CACCCTGGCA GGCACGCGGG GGCAAGTCCG GAGCGGCCTT CTACGCCACG 
GAAGATGATA GATTCATTTT GAAGCAGATG CCTCGTCTGG AAGTCCAGTC TTTCCTCGAC 
TTTGCACCAC ACTACTTCAA TTATATCACA AACGCTGTGC AACAAAAGAG GCCTACTGCA 
CTGGCCAAAA TTCTTGGAGT TTACAGAATT GGTTATAAGA ATTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGACTTGA AGGGTTCACT TAGGAACAGA AATGTAAAAA CTGACACCGG GAAAGAGAGC 
TGTGACGTGG TCCTGCTGGA CGAGAATCTG CTGAAGATGG TCCGGGACAG CCCTCTGTAC 
ATCCGCTCGC ACTCCAAGGC GGTGCTGCGG GCCTCCATCC GCAGCGATGC CCACTTCCTG 
TCCAGCCACC TCATCATCGA CTATTCTTTG CTGGTGGGGC GAGATGATAC CAGCAATGAG 
CTTGTCGTTG GGATTATAGA TTATATTCGA ACGTTTACAT GGGACAAAAA GCTTGAGATG 
GTTGTGAAAT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACTGT GGTCTCTCCA 
GAGTTGTACA GGACCCGATT TTGTGAAGCG ATGGACAAGT ATTTCCTCAT GGTACCAGAC 
CACTGGACAG GCTTGGGTCT CAACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_023622529.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023478297.1 Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X13, mRNA.

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CloneID	OHb52478
Clone ID Related Accession (Same CDS sequence)	XM_023622529.1
Accession Version	XM_023622529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009161.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGAGAA CAGTTTGCAG CACCCCCAGG AGAACACAGA CACAAGAAGG 
AAAACAGAAC CTACCTTTGG CGGTCATGAC CCTCGTACAG CTGTTCAGCT TCGAAGCCTC 
AGCACAGTGT TAAAACGCCT CAAGGAAATC ATGGAGGGGA AAAGCCAGGA CAGTGACCTG 
AAGCAGTACT GGATGCCGGA CAGCCAGTGC AGAGAGTGCT ACGACTGCAG CGAGAAGTTC 
ACCACCTTCC GGCGCAGGCA CCACTGCCGG CTCTGCGGGC AGATTTTCTG CAGCCGCTGC 
TGCAATCAAG AGATCCCTGG GAAGTTTATG GGCTATACAG GGGACCTCCG AGCTTGCACA 
TACTGTAGAA AAATAGCCTT AAGTTATGCT CATTCCACAG ACAGTAATTC CATTGGGGAA 
GACTTGAATG CTCTTTCAGA TTCTGCTTCC TCTGTGTCTG TACTTGATCC GAGTGAACCC 
CGAACACCTG TTGGGAGTAG GAAAGCCAGC CGGAACATCT TTTTAGAGGA TGATTTTGCC 
TGGCAAAGTT TGATTCATCC AGACTCCTCA AATACTGCTC TTTCAACAAG ACTTGTATCT 
GTTCAAGAGG ACGCTGGGAA ATCTCCTGCT CGAAATAGGT CAGCCAGCAT TACCAACCTC 
TCACTGGATC GATCTGGTTC TCCCCTGGTA CCTTCATATG AGACATCTGT CAGTCCCCAG 
GCCAACCGAA CATACGTGAG GACAGAGACC ACAGAGGATG AACGCAAAAT TCTTCTGGAC 
AGCGTCCAGT TAAAAGACCT GTGGAAGAAA ATCTGCCATC ACAGTAGTGG AATGGAGTTT 
CAGGATCACC GCTACTGGCT GAGGACACAT CCCAACTGCA TCGTAGGGAA GGAGTTAGTC 
AACTGGCTAA TCCGAAATGG GCACATTGCC ACAAGGGCAC AAGCTGTAGC AATTGGACAA 
GCGATGGTGG ACGGACGGTG GCTGGACTGC GTGAGCCACC ATGACCAGCT CTTCAGGGAT 
GAGTATGCGC TCTACAGACC CCTGCAGAGT ACAGACTTTT CTGAGACCCC TTCTCCGGAC 
AGTGACTCCG TGAACTCTGT GGAAGGACAC TCGGAGCCAT CATGGTTTAA AGACATAAAG 
TTTGATGACA GCGACACAGA GCAGATTGCT GAAGAAGGTG ACGATAATTT GACTAAGTAT 
TTGATTTCTG ACAACGGAGG ACAACAACTC TCAATAAGTG ACGCCTTCAT CAAAGAATCC 
TTATTTAATC GTCGAGTTGA GGAGAAATCC AAAGAACTGC CCTTTACGCC TTTGGGCTGG 
CATCATAACA ATCTGGAGCT GCTGCGGGAG GAGAATGGAG AGAAGCAGGC CATGGAGAGG 
TTGCTTTCAG CTAATCATAA CCACATGATG GCACTGCTCC AACAGTTGCT CCATAGTGAG 
TCATTGTCAC CATCTTGGAG GGACATCATT GTATCCCTGG TCTGCCAGGT TGTTCAGACA 
GTCCGACCTG ACGTCAAGAA CCGGGATGAC GACATGGATA TCCGTCAGTT TGTCCACGTC 
AAAAAAATCC CAGGTGGAAA GAAGTTTGAT TCTATGGTTG TCAATGGGTT CGTTTGTACC 
AAGAATATTG CACATAAAAA GATGAATTCT TGTATTAAAA ACCCCAAAAT TCTTCTGTTG 
AAGTGTTCCA TTGAGTATCT CTACAGAGAA GAAACTAAAT TTACTTGCAT TGACCCTATC 
GTGCTTCAGG AAAGGGAATT CTTGAAGAAT TATGTTCAGC GAATAGTGGA TGTTCGACCC 
ACATTGGTTC TAGTTGAGAA AACGGTGTCT CGGATTGCCC AGGACCTGTT GCTGGAACAC 
GGCATCACTT TGGTCATTAA TGTCAAATCA CAAGTTTTAG AACGAATCAG TCGGATGACC 
CAAGGTGATT TAGTGATGTC GATGGACCAG TTGCTCACAA AGCCCCACCT GGGCACCTGT 
CACAAATTTT ACATGCAGAT GTTTCAGCTA CCTGGTGAAC AAACCAAAAC TCTGATGTTT 
TTTGAAGGCT GTCCACAGCA CCTGGGCTGT ACAATCAAGC TTAGAGGAGG CTCCGATTAT 
GAGCTGGCTC GAGTTAAGGA GATCCTCATA TTTATGATCT GTGTAGCATA TCATTCTCAA 
CTAGAAATCT CCTTCCTCAT GGATGAATTT GCCATGCCTC CTACATTAAC GCAAAACCCT 
TCCTTCCACT CTCTGATTGA GGAACAGGAG GATGACGAGG CTGCTCAAGA ACAGCTCAGC 
GGAAGTCCCC TCCCCCGGGA ACCTGACTTC CCTCTGGAGT GTCTGGCCCC TGATGAGAGC 
AGTTCACTGG AATCAAGGAC TGTGTTTGGG AAGAGTGACC AGGAACTTAA AGGTGCTCCA 
AAGGATGCTG TCTCGTTGAA GCAGCAGGAG TTCGCCACAG CAGCCTGCCC GGCCGGCGTC 
CCCTATGCTC TCTTTGCATT GGTCCCAGAA TCCTTGTTGC CACTGCATCT GGGTGACCAA 
CAGGATGCCA GGGGCAGCGA GCAGCCAGAG ACTGTGCAGC AAACGGGTGA GCTACAGGAT 
CCCAAAAGCC AGATGAGAGC CTTTAGAGAC CCTCTGCAGG ATGACACTGG ATTGTATGTC 
ACTGAGGAAG TCACCTCTTC TGAGGATAAG CGGAAGACTT ACTCTTTGGC CTTTAAACAG 
GAATTAAAAG ACGTAATTCT CTGTATCTCC CCAGTGATCA CATTCCGAGA GCCTTTCCTT 
TTAACCGAGA AGGGGATGCG ATGCTCTCCC CGAGACTATT TCGCAGAGCA GCTTTACTGG 
TCTCCTCTCC TCAATAAAGA CTTCAAAGAA ATGGAGAGCA GGCGGAAGAA GCAGCTGCTC 
AGGGATCTCT CCAGCCTCCA GGGCCTGAAC GGAAGTGTGC AGGCCAAGTC TATCCAAGTG 
CTGCCCTCAC ACGAGCTGGT GAGCGCTAGG ATTGCTGAGC ATCTGGGTGA CAGCCAGGGC 
CTGGGCCGGA TGCTGGCTGA CTACCGGGCC AGAGGAGGGA GAATTCAGCA GAATACTTCA 
GACCCTTTTG CTCATTCAAA GGATGCATCA GGTCCTTCAA GTGGCAAGTC AGGAAGCAGA 
ACTGAGGGCG ATGAAGAGAA AGGGCTGATT CCGAGTGACG CGGTATGGTC AACAAAGGTG 
GACTGTCTGA ACCCTGCCAA CCACCAGAGG CTGTGCGTGC TCTTCAGCAG CTCTTCTGCC 
CAGTCCAGCA ACGCGCCCAG CGCCTGCGTC AGTCCTTGGA TTGTAACAAT GGAATTTTAT 
GGAAAGAATG ATCTTACACT GGGAATATTT TTAGAGAGAT ACTGTTTCAG GCCTTCTTAC 
CAGTGTCCTA GCATGTTCTG TGATACCCCC ATGGTACATC ACATTCGGCG CTTTGTCCAT 
GGCCAAGGCT GTGTGCAGAT AATCCTGAAG GAGCTGGATT CTCCAGTACC TGGATATCAG 
CATACAATTC TCACCTATTC CTGGTGCAGA ATCTGCAAAC AGGTAACACC AGTTGTTGCT 
CTTTCCAATG AGTCCTGGTC CATGTCATTT GCCAAATACC TTGAACTTCG GTTTTATGGG 
CACCAGTACA CCCGTAGAGC CAATGCTGAG CCGTGTGGCC ACTCCATCCA CCATGATTAT 
CACCAGTACT TCTCCTATAA CCAGATGGTG GCATCTTTTA GTTATTCTCC CATTCGGCTT 
CTTGAAGTAT GTGTTCCACT CCCCAAAATA TTCATTAAAC GTCAGGCCCC ATTAAAAGTG 
TCCCTTCTTC AAGATCTGAA GGACTTCTTT CAAAAAGTTT CACAAGTATA TCTTGCTGTT 
GATGAAAGAC TTGCATCTTT GAAAACTGAT ACATTTAGCA AAACAAGAGA GGAAAAAATG 
GAAGATATCT TTGCACAAAA AGAGATGGAG GAAGGCGAGT TCAAGAACTG GATTGAGAAA 
ATGCAAGCGA GGCTCCTGTC TTCCTCGGTA GACACCCCTC AGCAGCTGCA GTCGGTCCTG 
GAGTCGCTCA TCGCCAAGAA GCAGAGCCTC TGTGAAGTGC TGCAGGCCTG GAATAACAGG 
TTGCAGGACC TTTTCCAGCA GGAAAAGGGT AGAAAGCGGC CTTCAGTTCC TCCAAGTCCT 
GGAAGACTGA GACAAGGGGA AGAAAGCAAG ATAAGTGCAA TGGATGCATC TCCACGGAAT 
ATTTCTCCAG GACTTCAAAA TGGAGAAAAA GAGGACCGCT TCTTGACAAC CCTCTCTAGT 
CAGGGCCCCA CCTCTGCTGC TCATCTCCAG CTGCCCAGTC CCCCTGAAGT CCTGCCTGAG 
CAGGCAGGGG GGGCGACCCC CGAGCCGGAT GCAGCCAGCA GTTCTGAAGA TGTGTTTGAT 
GGACATTCTC TGGGATCCAC AGACAGCCAG GTGAAGGAAA AATCAACCAT GAAGGCCATC 
TTTGCAAATT TGCTTCCAGG AAATAGCTAT AACCCTATTC CGTTTCCTTT TGATCCAGAT 
AAACACTACT TAATGTACGA ACACGAACGG GTGCCCATTG CCGTCTGTGA GAGGGAGCCC 
AGCTCCATCA TTGCTTTTGC TCTCAGTTGT AAAGAATACC GAAATGCCTT AGAGGAATTG 
TCCAAGGCAA CTCTGCGGAA CAGTGCCGAA GAAGGGCTCC CAGCCAACAG TGCTTCAGAC 
AGCAGACCAA AGGGCGGCAG CCCTGGGAGG CCGCCTGAGC TCGGTGGAGG CTGCCCCAAC 
CGGGCGGCCG AGGCAGAGCC GCAGCCAGCC AAAAAGGCTT CTGGGATGTT GTCCTTCTTC 
CGAGGGACAG CAGGGAAAAG CCCTGATCTC TCTTCCCAGA AGAGAGAGAC CTTACGTGGA 
GCAGATAGTG CTTACTACCA GGTCGGGCAG CCCGGCAAGG AGGGGACGGA GAGTCAAGGC 
ACTGAGCCTC CAGATGAGAT AGATGGAGGA GACGCACAAA AGAAACAGCT CACAAATCCT 
CATGTGGAAC TTCAATTTTC TGACGCTAAC GCCAAGTTTT ACTGCCGGCT CTACTATGCG 
GGAGAGTTTC ACAAGATGCG CGAGGTGATC CTGGGCAGCA GCGAGGAGGA CTTCATCCGC 
TCCCTTTCCC ACTCGGCACC CTGGCAGGCA CGCGGGGGCA AGTCCGGAGC GGCCTTCTAC 
GCCACGGAAG ATGATAGATT CATTTTGAAG CAGATGCCTC GTCTGGAAGT CCAGTCTTTC 
CTCGACTTTG CACCACACTA CTTCAATTAT ATCACAAACG CTGTGCAACA AAAGAGGCCT 
ACTGCACTGG CCAAAATTCT TGGAGTTTAC AGAATTGGTT ATAAGAATTC TCAGAACAAC 
ACTGAGAAGA AGTTAGATCT CCTTGTCATG GAAAATCTTT TCTATGGGAG AAAGATGGCA 
CAGGTTTTTG ACTTGAAGGG TTCACTTAGG AACAGAAATG TAAAAACTGA CACCGGGAAA 
GAGAGCTGTG ACGTGGTCCT GCTGGACGAG AATCTGCTGA AGATGGTCCG GGACAGCCCT 
CTGTACATCC GCTCGCACTC CAAGGCGGTG CTGCGGGCCT CCATCCGCAG CGATGCCCAC 
TTCCTGTCCA GCCACCTCAT CATCGACTAT TCTTTGCTGG TGGGGCGAGA TGATACCAGC 
AATGAGCTTG TCGTTGGGAT TATAGATTAT ATTCGAACGT TTACATGGGA CAAAAAGCTT 
GAGATGGTTG TGAAATCAAC AGGAATTTTA GGAGGACAAG GTAAAATGCC AACTGTGGTC 
TCTCCAGAGT TGTACAGGAC CCGATTTTGT GAAGCGATGG ACAAGTATTT CCTCATGGTA 
CCAGACCACT GGACAGGCTT GGGTCTCAAC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023478297.1
CDS	114..6269
Translation	MATDDKTSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPAFKSAYSSFVNLFRFNKDRAEGEQQSLTGGWPSPQLPSRTQSVRSPIPYKKQLTEELQRRPSAVLENSLQHPQENTDTRRKTEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCRECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPLVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAVAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTDFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTKYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSPSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHVKKIPGGKKFDSMVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDLLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPGEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEEQEDDEAAQEQLSGSPLPREPDFPLECLAPDESSSLESRTVFGKSDQELKGAPKDAVSLKQQEFATAACPAGVPYALFALVPESLLPLHLGDQQDARGSEQPETVQQTGELQDPKSQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSPRDYFAEQLYWSPLLNKDFKEMESRRKKQLLRDLSSLQGLNGSVQAKSIQVLPSHELVSARIAEHLGDSQGLGRMLADYRARGGRIQQNTSDPFAHSKDASGPSSGKSGSRTEGDEEKGLIPSDAVWSTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLLSSSVDTPQQLQSVLESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQGPTSAAHLQLPSPPEVLPEQAGGATPEPDAASSSEDVFDGHSLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSASDSRPKGGSPGRPPELGGGCPNRAAEAEPQPAKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQPGKEGTESQGTEPPDEIDGGDAQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSAPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDSPLYIRSHSKAVLRASIRSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_023622529.1
Organism	Equus caballus(horse)
Definition	Equus caballus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023622529.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGAGAA CAGTTTGCAG CACCCCCAGG AGAACACAGA CACAAGAAGG 
AAAACAGAAC CTACCTTTGG CGGTCATGAC CCTCGTACAG CTGTTCAGCT TCGAAGCCTC 
AGCACAGTGT TAAAACGCCT CAAGGAAATC ATGGAGGGGA AAAGCCAGGA CAGTGACCTG 
AAGCAGTACT GGATGCCGGA CAGCCAGTGC AGAGAGTGCT ACGACTGCAG CGAGAAGTTC 
ACCACCTTCC GGCGCAGGCA CCACTGCCGG CTCTGCGGGC AGATTTTCTG CAGCCGCTGC 
TGCAATCAAG AGATCCCTGG GAAGTTTATG GGCTATACAG GGGACCTCCG AGCTTGCACA 
TACTGTAGAA AAATAGCCTT AAGTTATGCT CATTCCACAG ACAGTAATTC CATTGGGGAA 
GACTTGAATG CTCTTTCAGA TTCTGCTTCC TCTGTGTCTG TACTTGATCC GAGTGAACCC 
CGAACACCTG TTGGGAGTAG GAAAGCCAGC CGGAACATCT TTTTAGAGGA TGATTTTGCC 
TGGCAAAGTT TGATTCATCC AGACTCCTCA AATACTGCTC TTTCAACAAG ACTTGTATCT 
GTTCAAGAGG ACGCTGGGAA ATCTCCTGCT CGAAATAGGT CAGCCAGCAT TACCAACCTC 
TCACTGGATC GATCTGGTTC TCCCCTGGTA CCTTCATATG AGACATCTGT CAGTCCCCAG 
GCCAACCGAA CATACGTGAG GACAGAGACC ACAGAGGATG AACGCAAAAT TCTTCTGGAC 
AGCGTCCAGT TAAAAGACCT GTGGAAGAAA ATCTGCCATC ACAGTAGTGG AATGGAGTTT 
CAGGATCACC GCTACTGGCT GAGGACACAT CCCAACTGCA TCGTAGGGAA GGAGTTAGTC 
AACTGGCTAA TCCGAAATGG GCACATTGCC ACAAGGGCAC AAGCTGTAGC AATTGGACAA 
GCGATGGTGG ACGGACGGTG GCTGGACTGC GTGAGCCACC ATGACCAGCT CTTCAGGGAT 
GAGTATGCGC TCTACAGACC CCTGCAGAGT ACAGACTTTT CTGAGACCCC TTCTCCGGAC 
AGTGACTCCG TGAACTCTGT GGAAGGACAC TCGGAGCCAT CATGGTTTAA AGACATAAAG 
TTTGATGACA GCGACACAGA GCAGATTGCT GAAGAAGGTG ACGATAATTT GACTAAGTAT 
TTGATTTCTG ACAACGGAGG ACAACAACTC TCAATAAGTG ACGCCTTCAT CAAAGAATCC 
TTATTTAATC GTCGAGTTGA GGAGAAATCC AAAGAACTGC CCTTTACGCC TTTGGGCTGG 
CATCATAACA ATCTGGAGCT GCTGCGGGAG GAGAATGGAG AGAAGCAGGC CATGGAGAGG 
TTGCTTTCAG CTAATCATAA CCACATGATG GCACTGCTCC AACAGTTGCT CCATAGTGAG 
TCATTGTCAC CATCTTGGAG GGACATCATT GTATCCCTGG TCTGCCAGGT TGTTCAGACA 
GTCCGACCTG ACGTCAAGAA CCGGGATGAC GACATGGATA TCCGTCAGTT TGTCCACGTC 
AAAAAAATCC CAGGTGGAAA GAAGTTTGAT TCTATGGTTG TCAATGGGTT CGTTTGTACC 
AAGAATATTG CACATAAAAA GATGAATTCT TGTATTAAAA ACCCCAAAAT TCTTCTGTTG 
AAGTGTTCCA TTGAGTATCT CTACAGAGAA GAAACTAAAT TTACTTGCAT TGACCCTATC 
GTGCTTCAGG AAAGGGAATT CTTGAAGAAT TATGTTCAGC GAATAGTGGA TGTTCGACCC 
ACATTGGTTC TAGTTGAGAA AACGGTGTCT CGGATTGCCC AGGACCTGTT GCTGGAACAC 
GGCATCACTT TGGTCATTAA TGTCAAATCA CAAGTTTTAG AACGAATCAG TCGGATGACC 
CAAGGTGATT TAGTGATGTC GATGGACCAG TTGCTCACAA AGCCCCACCT GGGCACCTGT 
CACAAATTTT ACATGCAGAT GTTTCAGCTA CCTGGTGAAC AAACCAAAAC TCTGATGTTT 
TTTGAAGGCT GTCCACAGCA CCTGGGCTGT ACAATCAAGC TTAGAGGAGG CTCCGATTAT 
GAGCTGGCTC GAGTTAAGGA GATCCTCATA TTTATGATCT GTGTAGCATA TCATTCTCAA 
CTAGAAATCT CCTTCCTCAT GGATGAATTT GCCATGCCTC CTACATTAAC GCAAAACCCT 
TCCTTCCACT CTCTGATTGA GGAACAGGAG GATGACGAGG CTGCTCAAGA ACAGCTCAGC 
GGAAGTCCCC TCCCCCGGGA ACCTGACTTC CCTCTGGAGT GTCTGGCCCC TGATGAGAGC 
AGTTCACTGG AATCAAGGAC TGTGTTTGGG AAGAGTGACC AGGAACTTAA AGGTGCTCCA 
AAGGATGCTG TCTCGTTGAA GCAGCAGGAG TTCGCCACAG CAGCCTGCCC GGCCGGCGTC 
CCCTATGCTC TCTTTGCATT GGTCCCAGAA TCCTTGTTGC CACTGCATCT GGGTGACCAA 
CAGGATGCCA GGGGCAGCGA GCAGCCAGAG ACTGTGCAGC AAACGGGTGA GCTACAGGAT 
CCCAAAAGCC AGATGAGAGC CTTTAGAGAC CCTCTGCAGG ATGACACTGG ATTGTATGTC 
ACTGAGGAAG TCACCTCTTC TGAGGATAAG CGGAAGACTT ACTCTTTGGC CTTTAAACAG 
GAATTAAAAG ACGTAATTCT CTGTATCTCC CCAGTGATCA CATTCCGAGA GCCTTTCCTT 
TTAACCGAGA AGGGGATGCG ATGCTCTCCC CGAGACTATT TCGCAGAGCA GCTTTACTGG 
TCTCCTCTCC TCAATAAAGA CTTCAAAGAA ATGGAGAGCA GGCGGAAGAA GCAGCTGCTC 
AGGGATCTCT CCAGCCTCCA GGGCCTGAAC GGAAGTGTGC AGGCCAAGTC TATCCAAGTG 
CTGCCCTCAC ACGAGCTGGT GAGCGCTAGG ATTGCTGAGC ATCTGGGTGA CAGCCAGGGC 
CTGGGCCGGA TGCTGGCTGA CTACCGGGCC AGAGGAGGGA GAATTCAGCA GAATACTTCA 
GACCCTTTTG CTCATTCAAA GGATGCATCA GGTCCTTCAA GTGGCAAGTC AGGAAGCAGA 
ACTGAGGGCG ATGAAGAGAA AGGGCTGATT CCGAGTGACG CGGTATGGTC AACAAAGGTG 
GACTGTCTGA ACCCTGCCAA CCACCAGAGG CTGTGCGTGC TCTTCAGCAG CTCTTCTGCC 
CAGTCCAGCA ACGCGCCCAG CGCCTGCGTC AGTCCTTGGA TTGTAACAAT GGAATTTTAT 
GGAAAGAATG ATCTTACACT GGGAATATTT TTAGAGAGAT ACTGTTTCAG GCCTTCTTAC 
CAGTGTCCTA GCATGTTCTG TGATACCCCC ATGGTACATC ACATTCGGCG CTTTGTCCAT 
GGCCAAGGCT GTGTGCAGAT AATCCTGAAG GAGCTGGATT CTCCAGTACC TGGATATCAG 
CATACAATTC TCACCTATTC CTGGTGCAGA ATCTGCAAAC AGGTAACACC AGTTGTTGCT 
CTTTCCAATG AGTCCTGGTC CATGTCATTT GCCAAATACC TTGAACTTCG GTTTTATGGG 
CACCAGTACA CCCGTAGAGC CAATGCTGAG CCGTGTGGCC ACTCCATCCA CCATGATTAT 
CACCAGTACT TCTCCTATAA CCAGATGGTG GCATCTTTTA GTTATTCTCC CATTCGGCTT 
CTTGAAGTAT GTGTTCCACT CCCCAAAATA TTCATTAAAC GTCAGGCCCC ATTAAAAGTG 
TCCCTTCTTC AAGATCTGAA GGACTTCTTT CAAAAAGTTT CACAAGTATA TCTTGCTGTT 
GATGAAAGAC TTGCATCTTT GAAAACTGAT ACATTTAGCA AAACAAGAGA GGAAAAAATG 
GAAGATATCT TTGCACAAAA AGAGATGGAG GAAGGCGAGT TCAAGAACTG GATTGAGAAA 
ATGCAAGCGA GGCTCCTGTC TTCCTCGGTA GACACCCCTC AGCAGCTGCA GTCGGTCCTG 
GAGTCGCTCA TCGCCAAGAA GCAGAGCCTC TGTGAAGTGC TGCAGGCCTG GAATAACAGG 
TTGCAGGACC TTTTCCAGCA GGAAAAGGGT AGAAAGCGGC CTTCAGTTCC TCCAAGTCCT 
GGAAGACTGA GACAAGGGGA AGAAAGCAAG ATAAGTGCAA TGGATGCATC TCCACGGAAT 
ATTTCTCCAG GACTTCAAAA TGGAGAAAAA GAGGACCGCT TCTTGACAAC CCTCTCTAGT 
CAGGGCCCCA CCTCTGCTGC TCATCTCCAG CTGCCCAGTC CCCCTGAAGT CCTGCCTGAG 
CAGGCAGGGG GGGCGACCCC CGAGCCGGAT GCAGCCAGCA GTTCTGAAGA TGTGTTTGAT 
GGACATTCTC TGGGATCCAC AGACAGCCAG GTGAAGGAAA AATCAACCAT GAAGGCCATC 
TTTGCAAATT TGCTTCCAGG AAATAGCTAT AACCCTATTC CGTTTCCTTT TGATCCAGAT 
AAACACTACT TAATGTACGA ACACGAACGG GTGCCCATTG CCGTCTGTGA GAGGGAGCCC 
AGCTCCATCA TTGCTTTTGC TCTCAGTTGT AAAGAATACC GAAATGCCTT AGAGGAATTG 
TCCAAGGCAA CTCTGCGGAA CAGTGCCGAA GAAGGGCTCC CAGCCAACAG TGCTTCAGAC 
AGCAGACCAA AGGGCGGCAG CCCTGGGAGG CCGCCTGAGC TCGGTGGAGG CTGCCCCAAC 
CGGGCGGCCG AGGCAGAGCC GCAGCCAGCC AAAAAGGCTT CTGGGATGTT GTCCTTCTTC 
CGAGGGACAG CAGGGAAAAG CCCTGATCTC TCTTCCCAGA AGAGAGAGAC CTTACGTGGA 
GCAGATAGTG CTTACTACCA GGTCGGGCAG CCCGGCAAGG AGGGGACGGA GAGTCAAGGC 
ACTGAGCCTC CAGATGAGAT AGATGGAGGA GACGCACAAA AGAAACAGCT CACAAATCCT 
CATGTGGAAC TTCAATTTTC TGACGCTAAC GCCAAGTTTT ACTGCCGGCT CTACTATGCG 
GGAGAGTTTC ACAAGATGCG CGAGGTGATC CTGGGCAGCA GCGAGGAGGA CTTCATCCGC 
TCCCTTTCCC ACTCGGCACC CTGGCAGGCA CGCGGGGGCA AGTCCGGAGC GGCCTTCTAC 
GCCACGGAAG ATGATAGATT CATTTTGAAG CAGATGCCTC GTCTGGAAGT CCAGTCTTTC 
CTCGACTTTG CACCACACTA CTTCAATTAT ATCACAAACG CTGTGCAACA AAAGAGGCCT 
ACTGCACTGG CCAAAATTCT TGGAGTTTAC AGAATTGGTT ATAAGAATTC TCAGAACAAC 
ACTGAGAAGA AGTTAGATCT CCTTGTCATG GAAAATCTTT TCTATGGGAG AAAGATGGCA 
CAGGTTTTTG ACTTGAAGGG TTCACTTAGG AACAGAAATG TAAAAACTGA CACCGGGAAA 
GAGAGCTGTG ACGTGGTCCT GCTGGACGAG AATCTGCTGA AGATGGTCCG GGACAGCCCT 
CTGTACATCC GCTCGCACTC CAAGGCGGTG CTGCGGGCCT CCATCCGCAG CGATGCCCAC 
TTCCTGTCCA GCCACCTCAT CATCGACTAT TCTTTGCTGG TGGGGCGAGA TGATACCAGC 
AATGAGCTTG TCGTTGGGAT TATAGATTAT ATTCGAACGT TTACATGGGA CAAAAAGCTT 
GAGATGGTTG TGAAATCAAC AGGAATTTTA GGAGGACAAG GTAAAATGCC AACTGTGGTC 
TCTCCAGAGT TGTACAGGAC CCGATTTTGT GAAGCGATGG ACAAGTATTT CCTCATGGTA 
CCAGACCACT GGACAGGCTT GGGTCTCAAC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_001505123.3 ) cDNA ORF clone, Equus caballus(horse) -> XP_001505173.1 Equus caballus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X5, mRNA.

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CloneID	OHb08541
Clone ID Related Accession (Same CDS sequence)	XM_001505123.3
Accession Version	XM_001505123.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_001505123.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGACAC AAGAAGGAAA ACAGAACCTA CCTTTGGCGG TCATGACCCT 
CGTACAGCTG TTCAGCTTCG AAGCCTCAGC ACAGTGTTAA AACGCCTCAA GGAAATCATG 
GAGGGGAAAA GCCAGGACAG TGACCTGAAG CAGTACTGGA TGCCGGACAG CCAGTGCAGA 
GAGTGCTACG ACTGCAGCGA GAAGTTCACC ACCTTCCGGC GCAGGCACCA CTGCCGGCTC 
TGCGGGCAGA TTTTCTGCAG CCGCTGCTGC AATCAAGAGA TCCCTGGGAA GTTTATGGGC 
TATACAGGGG ACCTCCGAGC TTGCACATAC TGTAGAAAAA TAGCCTTAAG TTATGCTCAT 
TCCACAGACA GTAATTCCAT TGGGGAAGAC TTGAATGCTC TTTCAGATTC TGCTTCCTCT 
GTGTCTGTAC TTGATCCGAG TGAACCCCGA ACACCTGTTG GGAGTAGGAA AGCCAGCCGG 
AACATCTTTT TAGAGGATGA TTTTGCCTGG CAAAGTTTGA TTCATCCAGA CTCCTCAAAT 
ACTGCTCTTT CAACAAGACT TGTATCTGTT CAAGAGGACG CTGGGAAATC TCCTGCTCGA 
AATAGGTCAG CCAGCATTAC CAACCTCTCA CTGGATCGAT CTGGTTCTCC CCTGGTACCT 
TCATATGAGA CATCTGTCAG TCCCCAGGCC AACCGAACAT ACGTGAGGAC AGAGACCACA 
GAGGATGAAC GCAAAATTCT TCTGGACAGC GTCCAGTTAA AAGACCTGTG GAAGAAAATC 
TGCCATCACA GTAGTGGAAT GGAGTTTCAG GATCACCGCT ACTGGCTGAG GACACATCCC 
AACTGCATCG TAGGGAAGGA GTTAGTCAAC TGGCTAATCC GAAATGGGCA CATTGCCACA 
AGGGCACAAG CTGTAGCAAT TGGACAAGCG ATGGTGGACG GACGGTGGCT GGACTGCGTG 
AGCCACCATG ACCAGCTCTT CAGGGATGAG TATGCGCTCT ACAGACCCCT GCAGAGTACA 
GACTTTTCTG AGACCCCTTC TCCGGACAGT GACTCCGTGA ACTCTGTGGA AGGACACTCG 
GAGCCATCAT GGTTTAAAGA CATAAAGTTT GATGACAGCG ACACAGAGCA GATTGCTGAA 
GAAGGTGACG ATAATTTGAC TAACTCTGCC AGTCCTAGCA AGCGCACGTC AGTCAGCAGT 
TTCCAGTCCA CAGTGGACAG TGACTCAGCC GCCTCCATCA GCCTGAACGT GGAGTTGGAC 
AACGTGAACT TCCACATCAA GAAGCCCTCC AAGTACCCAC ACGTGCCCCC TCACCCTGCT 
GACCAAAAAG AGTATTTGAT TTCTGACAAC GGAGGACAAC AACTCTCAAT AAGTGACGCC 
TTCATCAAAG AATCCTTATT TAATCGTCGA GTTGAGGAGA AATCCAAAGA ACTGCCCTTT 
ACGCCTTTGG GCTGGCATCA TAACAATCTG GAGCTGCTGC GGGAGGAGAA TGGAGAGAAG 
CAGGCCATGG AGAGGTTGCT TTCAGCTAAT CATAACCACA TGATGGCACT GCTCCAACAG 
TTGCTCCATA GTGAGTCATT GTCACCATCT TGGAGGGACA TCATTGTATC CCTGGTCTGC 
CAGGTTGTTC AGACAGTCCG ACCTGACGTC AAGAACCGGG ATGACGACAT GGATATCCGT 
CAGTTTGTCC ACGTCAAAAA AATCCCAGGT GGAAAGAAGT TTGATTCTAT GGTTGTCAAT 
GGGTTCGTTT GTACCAAGAA TATTGCACAT AAAAAGATGA ATTCTTGTAT TAAAAACCCC 
AAAATTCTTC TGTTGAAGTG TTCCATTGAG TATCTCTACA GAGAAGAAAC TAAATTTACT 
TGCATTGACC CTATCGTGCT TCAGGAAAGG GAATTCTTGA AGAATTATGT TCAGCGAATA 
GTGGATGTTC GACCCACATT GGTTCTAGTT GAGAAAACGG TGTCTCGGAT TGCCCAGGAC 
CTGTTGCTGG AACACGGCAT CACTTTGGTC ATTAATGTCA AATCACAAGT TTTAGAACGA 
ATCAGTCGGA TGACCCAAGG TGATTTAGTG ATGTCGATGG ACCAGTTGCT CACAAAGCCC 
CACCTGGGCA CCTGTCACAA ATTTTACATG CAGATGTTTC AGCTACCTGG TGAACAAACC 
AAAACTCTGA TGTTTTTTGA AGGCTGTCCA CAGCACCTGG GCTGTACAAT CAAGCTTAGA 
GGAGGCTCCG ATTATGAGCT GGCTCGAGTT AAGGAGATCC TCATATTTAT GATCTGTGTA 
GCATATCATT CTCAACTAGA AATCTCCTTC CTCATGGATG AATTTGCCAT GCCTCCTACA 
TTAACGCAAA ACCCTTCCTT CCACTCTCTG ATTGAGGAAC AGGAGGATGA CGAGGCTGCT 
CAAGAACAGC TCAGCGGAAG TCCCCTCCCC CGGGAACCTG ACTTCCCTCT GGAGTGTCTG 
GCCCCTGATG AGAGCAGTTC ACTGGAATCA AGGACTGTGT TTGGGAAGAG TGACCAGGAA 
CTTAAAGGTG CTCCAAAGGA TGCTGTCTCG TTGAAGCAGC AGGAGTTCGC CACAGCAGCC 
TGCCCGGCCG GCGTCCCCTA TGCTCTCTTT GCATTGGTCC CAGAATCCTT GTTGCCACTG 
CATCTGGGTG ACCAACAGGA TGCCAGGGGC AGCGAGCAGC CAGAGACTGT GCAGCAAACG 
GGTGAGCTAC AGGATCCCAA AAGCCAGATG AGAGCCTTTA GAGACCCTCT GCAGGATGAC 
ACTGGATTGT ATGTCACTGA GGAAGTCACC TCTTCTGAGG ATAAGCGGAA GACTTACTCT 
TTGGCCTTTA AACAGGAATT AAAAGACGTA ATTCTCTGTA TCTCCCCAGT GATCACATTC 
CGAGAGCCTT TCCTTTTAAC CGAGAAGGGG ATGCGATGCT CTCCCCGAGA CTATTTCGCA 
GAGCAGCTTT ACTGGTCTCC TCTCCTCAAT AAAGACTTCA AAGAAATGGA GAGCAGGCGG 
AAGAAGCAGC TGCTCAGGGA TCTCTCCAGC CTCCAGGGCC TGAACGGAAG TGTGCAGGCC 
AAGTCTATCC AAGTGCTGCC CTCACACGAG CTGGTGAGCG CTAGGATTGC TGAGCATCTG 
GGTGACAGCC AGGGCCTGGG CCGGATGCTG GCTGACTACC GGGCCAGAGG AGGGAGAATT 
CAGCAGAATA CTTCAGACCC TTTTGCTCAT TCAAAGGATG CATCAGGTCC TTCAAGTGGC 
AAGTCAGGAA GCAGAACTGA GGGCGATGAA GAGAAAGGGC TGATTCCGAG TGACGCGGTA 
TGGTCAACAA AGGTGGACTG TCTGAACCCT GCCAACCACC AGAGGCTGTG CGTGCTCTTC 
AGCAGCTCTT CTGCCCAGTC CAGCAACGCG CCCAGCGCCT GCGTCAGTCC TTGGATTGTA 
ACAATGGAAT TTTATGGAAA GAATGATCTT ACACTGGGAA TATTTTTAGA GAGATACTGT 
TTCAGGCCTT CTTACCAGTG TCCTAGCATG TTCTGTGATA CCCCCATGGT ACATCACATT 
CGGCGCTTTG TCCATGGCCA AGGCTGTGTG CAGATAATCC TGAAGGAGCT GGATTCTCCA 
GTACCTGGAT ATCAGCATAC AATTCTCACC TATTCCTGGT GCAGAATCTG CAAACAGGTA 
ACACCAGTTG TTGCTCTTTC CAATGAGTCC TGGTCCATGT CATTTGCCAA ATACCTTGAA 
CTTCGGTTTT ATGGGCACCA GTACACCCGT AGAGCCAATG CTGAGCCGTG TGGCCACTCC 
ATCCACCATG ATTATCACCA GTACTTCTCC TATAACCAGA TGGTGGCATC TTTTAGTTAT 
TCTCCCATTC GGCTTCTTGA AGTATGTGTT CCACTCCCCA AAATATTCAT TAAACGTCAG 
GCCCCATTAA AAGTGTCCCT TCTTCAAGAT CTGAAGGACT TCTTTCAAAA AGTTTCACAA 
GTATATCTTG CTGTTGATGA AAGACTTGCA TCTTTGAAAA CTGATACATT TAGCAAAACA 
AGAGAGGAAA AAATGGAAGA TATCTTTGCA CAAAAAGAGA TGGAGGAAGG CGAGTTCAAG 
AACTGGATTG AGAAAATGCA AGCGAGGCTC CTGTCTTCCT CGGTAGACAC CCCTCAGCAG 
CTGCAGTCGG TCCTGGAGTC GCTCATCGCC AAGAAGCAGA GCCTCTGTGA AGTGCTGCAG 
GCCTGGAATA ACAGGTTGCA GGACCTTTTC CAGCAGGAAA AGGGTAGAAA GCGGCCTTCA 
GTTCCTCCAA GTCCTGGAAG ACTGAGACAA GGGGAAGAAA GCAAGATAAG TGCAATGGAT 
GCATCTCCAC GGAATATTTC TCCAGGACTT CAAAATGGAG AAAAAGAGGA CCGCTTCTTG 
ACAACCCTCT CTAGTCAGGG CCCCACCTCT GCTGCTCATC TCCAGCTGCC CAGTCCCCCT 
GAAGTCCTGC CTGAGCAGGC AGGGGGGGCG ACCCCCGAGC CGGATGCAGC CAGCAGTTCT 
GAAGATGTGT TTGATGGACA TTCTCTGGGA TCCACAGACA GCCAGGTGAA GGAAAAATCA 
ACCATGAAGG CCATCTTTGC AAATTTGCTT CCAGGAAATA GCTATAACCC TATCCGTACT 
CCAATGCCTT TTGATCCAGA TAAACACTAC TTAATGTACG AACACGAACG GGTGCCCATT 
GCCGTCTGTG AGAGGGAGCC CAGCTCCATC ATTGCTTTTG CTCTCAGTTG TAAAGAATAC 
CGAAATGCCT TAGAGGAATT GTCCAAGGCA ACTCTGCGGA ACAGTGCCGA AGAAGGGCTC 
CCAGCCAACA GTGCTTCAGA CAGCAGACCA AAGGGCGGCA GCCCTGGGAG GCCGCCTGAG 
CTCGGTGGAG GCTGCCCCAA CCGGGCGGCC GAGGCAGAGC CGCAGCCAGC CAAAAAGGCT 
TCTGGGATGT TGTCCTTCTT CCGAGGGACA GCAGGGAAAA GCCCTGATCT CTCTTCCCAG 
AAGAGAGAGA CCTTACGTGG AGCAGATAGT GCTTACTACC AGGTCGGGCA GCCCGGCAAG 
GAGGGGACGG AGAGTCAAGG CACTGAGCCT CCAGATGAGA TAGATGGAGG AGACGCACAA 
AAGAAACAGC TCACAAATCC TCATGTGGAA CTTCAATTTT CTGACGCTAA CGCCAAGTTT 
TACTGCCGGC TCTACTATGC GGGAGAGTTT CACAAGATGC GCGAGGTGAT CCTGGGCAGC 
AGCGAGGAGG ACTTCATCCG CTCCCTTTCC CACTCGGCAC CCTGGCAGGC ACGCGGGGGC 
AAGTCCGGAG CGGCCTTCTA CGCCACGGAA GATGATAGAT TCATTTTGAA GCAGATGCCT 
CGTCTGGAAG TCCAGTCTTT CCTCGACTTT GCACCACACT ACTTCAATTA TATCACAAAC 
GCTGTGCAAC AAAAGAGGCC TACTGCACTG GCCAAAATTC TTGGAGTTTA CAGAATTGGT 
TATAAGAATT CTCAGAACAA CACTGAGAAG AAGTTAGATC TCCTTGTCAT GGAAAATCTT 
TTCTATGGGA GAAAGATGGC ACAGGTTTTT GACTTGAAGG GTTCACTTAG GAACAGAAAT 
GTAAAAACTG ACACCGGGAA AGAGAGCTGT GACGTGGTCC TGCTGGACGA GAATCTGCTG 
AAGATGGTCC GGGACAGCCC TCTGTACATC CGCTCGCACT CCAAGGCGGT GCTGCGGGCC 
TCCATCCGCA GCGATGCCCA CTTCCTGTCC AGCCACCTCA TCATCGACTA TTCTTTGCTG 
GTGGGGCGAG ATGATACCAG CAATGAGCTT GTCGTTGGGA TTATAGATTA TATTCGAACG 
TTTACATGGG ACAAAAAGCT TGAGATGGTT GTGAAATCAA CAGGAATTTT AGGAGGACAA 
GGTAAAATGC CAACTGTGGT CTCTCCAGAG TTGTACAGGA CCCGATTTTG TGAAGCGATG 
GACAAGTATT TCCTCATGGT ACCAGACCAC TGGACAGGCT TGGGTCTCAA CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001505173.1
CDS	264..6560
Translation	MATDDKTSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPAFKSAYSSFVNLFRFNKDRAEGEQQSLTGGWPSPQLPSRTQSVRSPIPYKKQLTEELQRRPSAVLDTRRKTEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCRECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPLVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAVAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTDFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSPSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHVKKIPGGKKFDSMVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDLLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPGEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEEQEDDEAAQEQLSGSPLPREPDFPLECLAPDESSSLESRTVFGKSDQELKGAPKDAVSLKQQEFATAACPAGVPYALFALVPESLLPLHLGDQQDARGSEQPETVQQTGELQDPKSQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSPRDYFAEQLYWSPLLNKDFKEMESRRKKQLLRDLSSLQGLNGSVQAKSIQVLPSHELVSARIAEHLGDSQGLGRMLADYRARGGRIQQNTSDPFAHSKDASGPSSGKSGSRTEGDEEKGLIPSDAVWSTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLLSSSVDTPQQLQSVLESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQGPTSAAHLQLPSPPEVLPEQAGGATPEPDAASSSEDVFDGHSLGSTDSQVKEKSTMKAIFANLLPGNSYNPIRTPMPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSASDSRPKGGSPGRPPELGGGCPNRAAEAEPQPAKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQPGKEGTESQGTEPPDEIDGGDAQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSAPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDSPLYIRSHSKAVLRASIRSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_001505123.3
Organism	Equus caballus(horse)
Definition	Equus caballus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001505123.3

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCTGCTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCCGATCA GGACGAACCT 
GCTTTTAAGT CAGCATATAG TTCTTTTGTA AATCTCTTTC GATTTAACAA AGACAGAGCA 
GAGGGAGAGC AGCAGTCTCT GACTGGAGGT TGGCCCAGCC CTCAGCTGCC TTCAAGGACA 
CAGTCTGTGA GGTCACCTAT ACCCTATAAA AAGCAGCTTA CTGAGGAGCT CCAGCGGCGG 
CCTTCAGCAG TGCTGGACAC AAGAAGGAAA ACAGAACCTA CCTTTGGCGG TCATGACCCT 
CGTACAGCTG TTCAGCTTCG AAGCCTCAGC ACAGTGTTAA AACGCCTCAA GGAAATCATG 
GAGGGGAAAA GCCAGGACAG TGACCTGAAG CAGTACTGGA TGCCGGACAG CCAGTGCAGA 
GAGTGCTACG ACTGCAGCGA GAAGTTCACC ACCTTCCGGC GCAGGCACCA CTGCCGGCTC 
TGCGGGCAGA TTTTCTGCAG CCGCTGCTGC AATCAAGAGA TCCCTGGGAA GTTTATGGGC 
TATACAGGGG ACCTCCGAGC TTGCACATAC TGTAGAAAAA TAGCCTTAAG TTATGCTCAT 
TCCACAGACA GTAATTCCAT TGGGGAAGAC TTGAATGCTC TTTCAGATTC TGCTTCCTCT 
GTGTCTGTAC TTGATCCGAG TGAACCCCGA ACACCTGTTG GGAGTAGGAA AGCCAGCCGG 
AACATCTTTT TAGAGGATGA TTTTGCCTGG CAAAGTTTGA TTCATCCAGA CTCCTCAAAT 
ACTGCTCTTT CAACAAGACT TGTATCTGTT CAAGAGGACG CTGGGAAATC TCCTGCTCGA 
AATAGGTCAG CCAGCATTAC CAACCTCTCA CTGGATCGAT CTGGTTCTCC CCTGGTACCT 
TCATATGAGA CATCTGTCAG TCCCCAGGCC AACCGAACAT ACGTGAGGAC AGAGACCACA 
GAGGATGAAC GCAAAATTCT TCTGGACAGC GTCCAGTTAA AAGACCTGTG GAAGAAAATC 
TGCCATCACA GTAGTGGAAT GGAGTTTCAG GATCACCGCT ACTGGCTGAG GACACATCCC 
AACTGCATCG TAGGGAAGGA GTTAGTCAAC TGGCTAATCC GAAATGGGCA CATTGCCACA 
AGGGCACAAG CTGTAGCAAT TGGACAAGCG ATGGTGGACG GACGGTGGCT GGACTGCGTG 
AGCCACCATG ACCAGCTCTT CAGGGATGAG TATGCGCTCT ACAGACCCCT GCAGAGTACA 
GACTTTTCTG AGACCCCTTC TCCGGACAGT GACTCCGTGA ACTCTGTGGA AGGACACTCG 
GAGCCATCAT GGTTTAAAGA CATAAAGTTT GATGACAGCG ACACAGAGCA GATTGCTGAA 
GAAGGTGACG ATAATTTGAC TAACTCTGCC AGTCCTAGCA AGCGCACGTC AGTCAGCAGT 
TTCCAGTCCA CAGTGGACAG TGACTCAGCC GCCTCCATCA GCCTGAACGT GGAGTTGGAC 
AACGTGAACT TCCACATCAA GAAGCCCTCC AAGTACCCAC ACGTGCCCCC TCACCCTGCT 
GACCAAAAAG AGTATTTGAT TTCTGACAAC GGAGGACAAC AACTCTCAAT AAGTGACGCC 
TTCATCAAAG AATCCTTATT TAATCGTCGA GTTGAGGAGA AATCCAAAGA ACTGCCCTTT 
ACGCCTTTGG GCTGGCATCA TAACAATCTG GAGCTGCTGC GGGAGGAGAA TGGAGAGAAG 
CAGGCCATGG AGAGGTTGCT TTCAGCTAAT CATAACCACA TGATGGCACT GCTCCAACAG 
TTGCTCCATA GTGAGTCATT GTCACCATCT TGGAGGGACA TCATTGTATC CCTGGTCTGC 
CAGGTTGTTC AGACAGTCCG ACCTGACGTC AAGAACCGGG ATGACGACAT GGATATCCGT 
CAGTTTGTCC ACGTCAAAAA AATCCCAGGT GGAAAGAAGT TTGATTCTAT GGTTGTCAAT 
GGGTTCGTTT GTACCAAGAA TATTGCACAT AAAAAGATGA ATTCTTGTAT TAAAAACCCC 
AAAATTCTTC TGTTGAAGTG TTCCATTGAG TATCTCTACA GAGAAGAAAC TAAATTTACT 
TGCATTGACC CTATCGTGCT TCAGGAAAGG GAATTCTTGA AGAATTATGT TCAGCGAATA 
GTGGATGTTC GACCCACATT GGTTCTAGTT GAGAAAACGG TGTCTCGGAT TGCCCAGGAC 
CTGTTGCTGG AACACGGCAT CACTTTGGTC ATTAATGTCA AATCACAAGT TTTAGAACGA 
ATCAGTCGGA TGACCCAAGG TGATTTAGTG ATGTCGATGG ACCAGTTGCT CACAAAGCCC 
CACCTGGGCA CCTGTCACAA ATTTTACATG CAGATGTTTC AGCTACCTGG TGAACAAACC 
AAAACTCTGA TGTTTTTTGA AGGCTGTCCA CAGCACCTGG GCTGTACAAT CAAGCTTAGA 
GGAGGCTCCG ATTATGAGCT GGCTCGAGTT AAGGAGATCC TCATATTTAT GATCTGTGTA 
GCATATCATT CTCAACTAGA AATCTCCTTC CTCATGGATG AATTTGCCAT GCCTCCTACA 
TTAACGCAAA ACCCTTCCTT CCACTCTCTG ATTGAGGAAC AGGAGGATGA CGAGGCTGCT 
CAAGAACAGC TCAGCGGAAG TCCCCTCCCC CGGGAACCTG ACTTCCCTCT GGAGTGTCTG 
GCCCCTGATG AGAGCAGTTC ACTGGAATCA AGGACTGTGT TTGGGAAGAG TGACCAGGAA 
CTTAAAGGTG CTCCAAAGGA TGCTGTCTCG TTGAAGCAGC AGGAGTTCGC CACAGCAGCC 
TGCCCGGCCG GCGTCCCCTA TGCTCTCTTT GCATTGGTCC CAGAATCCTT GTTGCCACTG 
CATCTGGGTG ACCAACAGGA TGCCAGGGGC AGCGAGCAGC CAGAGACTGT GCAGCAAACG 
GGTGAGCTAC AGGATCCCAA AAGCCAGATG AGAGCCTTTA GAGACCCTCT GCAGGATGAC 
ACTGGATTGT ATGTCACTGA GGAAGTCACC TCTTCTGAGG ATAAGCGGAA GACTTACTCT 
TTGGCCTTTA AACAGGAATT AAAAGACGTA ATTCTCTGTA TCTCCCCAGT GATCACATTC 
CGAGAGCCTT TCCTTTTAAC CGAGAAGGGG ATGCGATGCT CTCCCCGAGA CTATTTCGCA 
GAGCAGCTTT ACTGGTCTCC TCTCCTCAAT AAAGACTTCA AAGAAATGGA GAGCAGGCGG 
AAGAAGCAGC TGCTCAGGGA TCTCTCCAGC CTCCAGGGCC TGAACGGAAG TGTGCAGGCC 
AAGTCTATCC AAGTGCTGCC CTCACACGAG CTGGTGAGCG CTAGGATTGC TGAGCATCTG 
GGTGACAGCC AGGGCCTGGG CCGGATGCTG GCTGACTACC GGGCCAGAGG AGGGAGAATT 
CAGCAGAATA CTTCAGACCC TTTTGCTCAT TCAAAGGATG CATCAGGTCC TTCAAGTGGC 
AAGTCAGGAA GCAGAACTGA GGGCGATGAA GAGAAAGGGC TGATTCCGAG TGACGCGGTA 
TGGTCAACAA AGGTGGACTG TCTGAACCCT GCCAACCACC AGAGGCTGTG CGTGCTCTTC 
AGCAGCTCTT CTGCCCAGTC CAGCAACGCG CCCAGCGCCT GCGTCAGTCC TTGGATTGTA 
ACAATGGAAT TTTATGGAAA GAATGATCTT ACACTGGGAA TATTTTTAGA GAGATACTGT 
TTCAGGCCTT CTTACCAGTG TCCTAGCATG TTCTGTGATA CCCCCATGGT ACATCACATT 
CGGCGCTTTG TCCATGGCCA AGGCTGTGTG CAGATAATCC TGAAGGAGCT GGATTCTCCA 
GTACCTGGAT ATCAGCATAC AATTCTCACC TATTCCTGGT GCAGAATCTG CAAACAGGTA 
ACACCAGTTG TTGCTCTTTC CAATGAGTCC TGGTCCATGT CATTTGCCAA ATACCTTGAA 
CTTCGGTTTT ATGGGCACCA GTACACCCGT AGAGCCAATG CTGAGCCGTG TGGCCACTCC 
ATCCACCATG ATTATCACCA GTACTTCTCC TATAACCAGA TGGTGGCATC TTTTAGTTAT 
TCTCCCATTC GGCTTCTTGA AGTATGTGTT CCACTCCCCA AAATATTCAT TAAACGTCAG 
GCCCCATTAA AAGTGTCCCT TCTTCAAGAT CTGAAGGACT TCTTTCAAAA AGTTTCACAA 
GTATATCTTG CTGTTGATGA AAGACTTGCA TCTTTGAAAA CTGATACATT TAGCAAAACA 
AGAGAGGAAA AAATGGAAGA TATCTTTGCA CAAAAAGAGA TGGAGGAAGG CGAGTTCAAG 
AACTGGATTG AGAAAATGCA AGCGAGGCTC CTGTCTTCCT CGGTAGACAC CCCTCAGCAG 
CTGCAGTCGG TCCTGGAGTC GCTCATCGCC AAGAAGCAGA GCCTCTGTGA AGTGCTGCAG 
GCCTGGAATA ACAGGTTGCA GGACCTTTTC CAGCAGGAAA AGGGTAGAAA GCGGCCTTCA 
GTTCCTCCAA GTCCTGGAAG ACTGAGACAA GGGGAAGAAA GCAAGATAAG TGCAATGGAT 
GCATCTCCAC GGAATATTTC TCCAGGACTT CAAAATGGAG AAAAAGAGGA CCGCTTCTTG 
ACAACCCTCT CTAGTCAGGG CCCCACCTCT GCTGCTCATC TCCAGCTGCC CAGTCCCCCT 
GAAGTCCTGC CTGAGCAGGC AGGGGGGGCG ACCCCCGAGC CGGATGCAGC CAGCAGTTCT 
GAAGATGTGT TTGATGGACA TTCTCTGGGA TCCACAGACA GCCAGGTGAA GGAAAAATCA 
ACCATGAAGG CCATCTTTGC AAATTTGCTT CCAGGAAATA GCTATAACCC TATCCGTACT 
CCAATGCCTT TTGATCCAGA TAAACACTAC TTAATGTACG AACACGAACG GGTGCCCATT 
GCCGTCTGTG AGAGGGAGCC CAGCTCCATC ATTGCTTTTG CTCTCAGTTG TAAAGAATAC 
CGAAATGCCT TAGAGGAATT GTCCAAGGCA ACTCTGCGGA ACAGTGCCGA AGAAGGGCTC 
CCAGCCAACA GTGCTTCAGA CAGCAGACCA AAGGGCGGCA GCCCTGGGAG GCCGCCTGAG 
CTCGGTGGAG GCTGCCCCAA CCGGGCGGCC GAGGCAGAGC CGCAGCCAGC CAAAAAGGCT 
TCTGGGATGT TGTCCTTCTT CCGAGGGACA GCAGGGAAAA GCCCTGATCT CTCTTCCCAG 
AAGAGAGAGA CCTTACGTGG AGCAGATAGT GCTTACTACC AGGTCGGGCA GCCCGGCAAG 
GAGGGGACGG AGAGTCAAGG CACTGAGCCT CCAGATGAGA TAGATGGAGG AGACGCACAA 
AAGAAACAGC TCACAAATCC TCATGTGGAA CTTCAATTTT CTGACGCTAA CGCCAAGTTT 
TACTGCCGGC TCTACTATGC GGGAGAGTTT CACAAGATGC GCGAGGTGAT CCTGGGCAGC 
AGCGAGGAGG ACTTCATCCG CTCCCTTTCC CACTCGGCAC CCTGGCAGGC ACGCGGGGGC 
AAGTCCGGAG CGGCCTTCTA CGCCACGGAA GATGATAGAT TCATTTTGAA GCAGATGCCT 
CGTCTGGAAG TCCAGTCTTT CCTCGACTTT GCACCACACT ACTTCAATTA TATCACAAAC 
GCTGTGCAAC AAAAGAGGCC TACTGCACTG GCCAAAATTC TTGGAGTTTA CAGAATTGGT 
TATAAGAATT CTCAGAACAA CACTGAGAAG AAGTTAGATC TCCTTGTCAT GGAAAATCTT 
TTCTATGGGA GAAAGATGGC ACAGGTTTTT GACTTGAAGG GTTCACTTAG GAACAGAAAT 
GTAAAAACTG ACACCGGGAA AGAGAGCTGT GACGTGGTCC TGCTGGACGA GAATCTGCTG 
AAGATGGTCC GGGACAGCCC TCTGTACATC CGCTCGCACT CCAAGGCGGT GCTGCGGGCC 
TCCATCCGCA GCGATGCCCA CTTCCTGTCC AGCCACCTCA TCATCGACTA TTCTTTGCTG 
GTGGGGCGAG ATGATACCAG CAATGAGCTT GTCGTTGGGA TTATAGATTA TATTCGAACG 
TTTACATGGG ACAAAAAGCT TGAGATGGTT GTGAAATCAA CAGGAATTTT AGGAGGACAA 
GGTAAAATGC CAACTGTGGT CTCTCCAGAG TTGTACAGGA CCCGATTTTG TGAAGCGATG 
GACAAGTATT TCCTCATGGT ACCAGACCAC TGGACAGGCT TGGGTCTCAA CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PIKFYVE
Entrez Gene ID	100066445
Full Name	phosphoinositide kinase, FYVE-type zinc finger containing
General protein information	Preferred Names phosphoinositide kinase, FYVE-type zinc finger containing Names 1-phosphatidylinositol 3-phosphate 5-kinase
Gene Type	protein-coding
Organism	Equus caballus(horse)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PIKFYVE cDNA ORF clone, Equus caballus(horse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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