LOC101386988 cDNA ORF clone, Odobenus rosmarus divergens(Pacific walrus)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101386988
Entrez Gene ID	101386988
Full Name	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
Synonyms	TYW1
Gene Type	protein-coding
Organism	Odobenus rosmarus divergens(Pacific walrus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004405411.2	XP_004405468.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase

Pathways from BioSystems
Gene Ontology

The following LOC101386988 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101386988 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOd59011	XM_004405411.2 Latest version!	Odobenus rosmarus divergens S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase (LOC101386988), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101386988 ( XM_004405411.2 ) cDNA ORF clone, Odobenus rosmarus divergens(Pacific walrus) -> XP_004405468.1 Odobenus rosmarus divergens S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase (LOC101386988), mRNA.

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CloneID	OOd59011
Clone ID Related Accession (Same CDS sequence)	XM_004405411.2
Accession Version	XM_004405411.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Odobenus rosmarus divergens(Pacific walrus)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004450510.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 26, 2015 this sequence version replaced XM_004405411.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Odobenus rosmarus divergens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTT TGGATACATG GGACTTCTCA TCATCTTTAA TGTCATTATG GATAAACAGG 
TTTTACGTCT ATTTGGGCTT TGCCTTTGGC ATTAGCCTTT GGATTTGTTT CCAAATTGTC 
ATCAGGAAGC AGTTGTTTAT CTTCAAGGAC AAGAACTCAC AGAAATCTGT TCCAAAAGCA 
ACTGGGGATT TGATGAAAAA TGGTTATATC TCTCTTCAAA AGAAGGAAGT CTTTGTGTCC 
GGAGTGAAGA TTTTCTATGG TTCCCAGACT GGAACAGCAA AGGGATTTGC AACAAGTCTT 
GCTGAAGCAG TTACCTCCCT AGATCTGCCT GTGGCTGTTA TTAATCTGAA AGAATATGAT 
CCAGATGATC ATCTTGTAGA GGAGGTTACT AGTAAAAACG TTTGTGCCTT TGTGGTTGCT 
ACATATACTG ATGGTCGACC CACTGAAAGT GCAGAGTGGT TCTGCAAATG GTTGGAGGAA 
GCATCCAGTG ATTTTCGATT TGGCAAAACT TACCTGAAGG GTATGAGGTA CGCTGTATTT 
GGCCTGGGAA ATTCTGCCTA TGCGAGCCAC TTCAACAAGG TTGGCAAGAA TGTCGATAAG 
TGGCTCTGGA TGCTTGGTGC TCATCGTGTG GTGACTAGAG GGGAGGGGGA CTGTGACGTG 
GCTAAGAGCA AACACGGCAG CATCGAAGCA GACTTCAGAG CCTGGAAGAC CAAGTTCCTT 
GCCCGGCTGC AGACACTTCA GAAAGGAGAG AAAAAGCCCT GTGACGGCAA CTGCAAGAGA 
GGCCAGTGCG AGTCGTCTGC GGAGGAAGAG CCCTTTGAGA GCACCAGTGA GGAAGAGTCT 
GGTACTGAAG ACCATCAGAA TCTAAATTCT GTTGTTGATG TTGAGGACCT CGGCAAAATT 
ATGAATTATG TGAAGAAAGA AAAGAGAGAA AATGAGCAAC AGGAGGAGAA AAGTAGTCTG 
GTCAGGAACA CGGTGAAGAA TGAAGCCAGT GAAAGAAGAG CTATGATAAC CCCTGCTCTC 
CGAGAAGCCC TCACCAAACA AGGGTATCAG TTGATTGGGA GCCACTCTGG AGTGAAGCTT 
TGCAGATGGA CAAAGTCGAT GCTCCGAGGG AGAGGAGGTT GCTATAAACA TACATTCTAT 
GGTATTGAAA GCCATTGTTG CATGGAAACT ACCCCAAGCT TGGCTTGTGC AAATAAATGT 
GTCTTCTGTT GGCGGCACCA CACCAATCCA GTGGGCACCG AATGGCGGTG GAAGATGGAC 
CAGCCTGAAA TGATCTTAAA GGAAGCCATT GAAAACCATC AGAACATGAT TAAGCAGTTT 
AAAGGAGTAC CAGGTGTCAA AGAAGAACGT TTTGAAGAGG GGATGATGGT AAAGCACTGT 
GCGTTGTCCC TTGTGGGAGA ACCCATCATG TACCCAGAAA TCAATAGGTT TTTGAAACTA 
CTCCATGAGT GTAAAATCTC CAGTTTCCTG GTCACAAATG CACAATTCCC TGTGGAAATC 
AGGAACCTCA AACCAGTTAC GCAGCTATAT GTCAGTGTGG ATGCTAGCAC CAAAGATAGC 
CTGAAGAAAA TCGACCGCCC ACTCTTCAAG GATTTCTGGC AGAGATTCCT CGACAGCTTA 
AAAGCCTTGG CAACAAAGCA ACAGCGTACT GTGTACAGAC TGACTCTAGT GAAAGCATGG 
AATGTGGATG AACTCCAGGC CTATGCTGAG CTGGTGTCCC TGGGGAATCC CGACTTCATC 
GAAGTGAAGG GTGTCACCTA CTGTGGGGAG AGTTCAGCAA GCAGTCTCAC CATGGCCAAT 
GTGCCCTGGC ACGAGGAGGT GATACACTTT GTCCGTGACT TGGTGGATCT GATCCCCGAC 
TATGAAATTG CTTGTGAACA TGAACATTCC AACTGCCTCC TGATAGCTCA CAAAAGGTTT 
AAGATTGATA GCGAGTGGTG GACATGGATC GATTATAACC GCTTCCAGGA GCTCGTGCAG 
GAATATGAAG ACAGTGGCGG ATCAGCAACT TTCAGCGCAA AGGATTATGT GGCGAAAACT 
CCTCACTGGG CATTATTTGG TGCCCGTGAA AGAGGCTTTG ATCCCAAGGA CACAAGACAT 
CAGAGAAAGA ACAAATCAAA GCATATTTCC GGATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004405468.1
CDS	162..2300
Translation	MDPLDTWDFSSSLMSLWINRFYVYLGFAFGISLWICFQIVIRKQLFIFKDKNSQKSVPKATGDLMKNGYISLQKKEVFVSGVKIFYGSQTGTAKGFATSLAEAVTSLDLPVAVINLKEYDPDDHLVEEVTSKNVCAFVVATYTDGRPTESAEWFCKWLEEASSDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWLWMLGAHRVVTRGEGDCDVAKSKHGSIEADFRAWKTKFLARLQTLQKGEKKPCDGNCKRGQCESSAEEEPFESTSEEESGTEDHQNLNSVVDVEDLGKIMNYVKKEKRENEQQEEKSSLVRNTVKNEASERRAMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHCCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILKEAIENHQNMIKQFKGVPGVKEERFEEGMMVKHCALSLVGEPIMYPEINRFLKLLHECKISSFLVTNAQFPVEIRNLKPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLKALATKQQRTVYRLTLVKAWNVDELQAYAELVSLGNPDFIEVKGVTYCGESSASSLTMANVPWHEEVIHFVRDLVDLIPDYEIACEHEHSNCLLIAHKRFKIDSEWWTWIDYNRFQELVQEYEDSGGSATFSAKDYVAKTPHWALFGARERGFDPKDTRHQRKNKSKHISGC

Target ORF information:

RefSeq Version	XM_004405411.2
Organism	Odobenus rosmarus divergens(Pacific walrus)
Definition	Odobenus rosmarus divergens S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase (LOC101386988), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004405411.2

ORF Insert Sequence:

ATGGATCCTT TGGATACATG GGACTTCTCA TCATCTTTAA TGTCATTATG GATAAACAGG 
TTTTACGTCT ATTTGGGCTT TGCCTTTGGC ATTAGCCTTT GGATTTGTTT CCAAATTGTC 
ATCAGGAAGC AGTTGTTTAT CTTCAAGGAC AAGAACTCAC AGAAATCTGT TCCAAAAGCA 
ACTGGGGATT TGATGAAAAA TGGTTATATC TCTCTTCAAA AGAAGGAAGT CTTTGTGTCC 
GGAGTGAAGA TTTTCTATGG TTCCCAGACT GGAACAGCAA AGGGATTTGC AACAAGTCTT 
GCTGAAGCAG TTACCTCCCT AGATCTGCCT GTGGCTGTTA TTAATCTGAA AGAATATGAT 
CCAGATGATC ATCTTGTAGA GGAGGTTACT AGTAAAAACG TTTGTGCCTT TGTGGTTGCT 
ACATATACTG ATGGTCGACC CACTGAAAGT GCAGAGTGGT TCTGCAAATG GTTGGAGGAA 
GCATCCAGTG ATTTTCGATT TGGCAAAACT TACCTGAAGG GTATGAGGTA CGCTGTATTT 
GGCCTGGGAA ATTCTGCCTA TGCGAGCCAC TTCAACAAGG TTGGCAAGAA TGTCGATAAG 
TGGCTCTGGA TGCTTGGTGC TCATCGTGTG GTGACTAGAG GGGAGGGGGA CTGTGACGTG 
GCTAAGAGCA AACACGGCAG CATCGAAGCA GACTTCAGAG CCTGGAAGAC CAAGTTCCTT 
GCCCGGCTGC AGACACTTCA GAAAGGAGAG AAAAAGCCCT GTGACGGCAA CTGCAAGAGA 
GGCCAGTGCG AGTCGTCTGC GGAGGAAGAG CCCTTTGAGA GCACCAGTGA GGAAGAGTCT 
GGTACTGAAG ACCATCAGAA TCTAAATTCT GTTGTTGATG TTGAGGACCT CGGCAAAATT 
ATGAATTATG TGAAGAAAGA AAAGAGAGAA AATGAGCAAC AGGAGGAGAA AAGTAGTCTG 
GTCAGGAACA CGGTGAAGAA TGAAGCCAGT GAAAGAAGAG CTATGATAAC CCCTGCTCTC 
CGAGAAGCCC TCACCAAACA AGGGTATCAG TTGATTGGGA GCCACTCTGG AGTGAAGCTT 
TGCAGATGGA CAAAGTCGAT GCTCCGAGGG AGAGGAGGTT GCTATAAACA TACATTCTAT 
GGTATTGAAA GCCATTGTTG CATGGAAACT ACCCCAAGCT TGGCTTGTGC AAATAAATGT 
GTCTTCTGTT GGCGGCACCA CACCAATCCA GTGGGCACCG AATGGCGGTG GAAGATGGAC 
CAGCCTGAAA TGATCTTAAA GGAAGCCATT GAAAACCATC AGAACATGAT TAAGCAGTTT 
AAAGGAGTAC CAGGTGTCAA AGAAGAACGT TTTGAAGAGG GGATGATGGT AAAGCACTGT 
GCGTTGTCCC TTGTGGGAGA ACCCATCATG TACCCAGAAA TCAATAGGTT TTTGAAACTA 
CTCCATGAGT GTAAAATCTC CAGTTTCCTG GTCACAAATG CACAATTCCC TGTGGAAATC 
AGGAACCTCA AACCAGTTAC GCAGCTATAT GTCAGTGTGG ATGCTAGCAC CAAAGATAGC 
CTGAAGAAAA TCGACCGCCC ACTCTTCAAG GATTTCTGGC AGAGATTCCT CGACAGCTTA 
AAAGCCTTGG CAACAAAGCA ACAGCGTACT GTGTACAGAC TGACTCTAGT GAAAGCATGG 
AATGTGGATG AACTCCAGGC CTATGCTGAG CTGGTGTCCC TGGGGAATCC CGACTTCATC 
GAAGTGAAGG GTGTCACCTA CTGTGGGGAG AGTTCAGCAA GCAGTCTCAC CATGGCCAAT 
GTGCCCTGGC ACGAGGAGGT GATACACTTT GTCCGTGACT TGGTGGATCT GATCCCCGAC 
TATGAAATTG CTTGTGAACA TGAACATTCC AACTGCCTCC TGATAGCTCA CAAAAGGTTT 
AAGATTGATA GCGAGTGGTG GACATGGATC GATTATAACC GCTTCCAGGA GCTCGTGCAG 
GAATATGAAG ACAGTGGCGG ATCAGCAACT TTCAGCGCAA AGGATTATGT GGCGAAAACT 
CCTCACTGGG CATTATTTGG TGCCCGTGAA AGAGGCTTTG ATCCCAAGGA CACAAGACAT 
CAGAGAAAGA ACAAATCAAA GCATATTTCC GGATGTTGA