VIM cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	VIM
Entrez Gene ID	477991
Full Name	vimentin
General protein information	Preferred Names vimentin Names vimentin
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001287023.1	NP_001273952.1	vimentin

Pathways from BioSystems

Homologies

Bos taurus (cattle)	VIM	NP_776394.2
Mus musculus (house mouse)	Vim	NP_035831.2
Danio rerio (zebrafish)	vim	NP_571947.1
Pan troglodytes (chimpanzee)	VIM	NP_001009148.1
Xenopus tropicalis (tropical clawed frog)	vim	NP_001128275.1
Canis lupus familiaris (dog)	VIM	XP_856478.1
Homo sapiens (human)	VIM	NP_003371.2
Rattus norvegicus (Norway rat)	Vim	NP_112402.1
Gallus gallus (chicken)	VIM	NP_001041541.1

Gene Ontology
Bibliography

Related articles in PubMed

Filamin acts as a key regulator in epithelial defence against transformed cells.
Kajita M, Sugimura K, Ohoka A, Burden J, Suganuma H, Ikegawa M, Shimada T, Kitamura T, Shindoh M, Ishikawa S, Yamamoto S, Saitoh S, Yako Y, Takahashi R, Okajima T, Kikuta J, Maijima Y, Ishii M, Tada M, Fujita Y
Nature communications54428(2014 Jul)

A simple and rapid immunocytochemical technique for detection of cytokeratin, vimentin, and S-100 protein in veterinary diagnostic cytology.
Sawa M, Yabuki A, Miyoshi N, Arai K, Yamato O
Research in veterinary science93(3)1341-5(2012 Dec)

Vimentin and the K-Ras-induced actin-binding protein control inositol-(1,4,5)-trisphosphate receptor redistribution during MDCK cell differentiation.
Dingli F, Parys JB, Loew D, Saule S, Mery L
Journal of cell science125(Pt 22)5428-40(2012 Nov)

Cdc42GAP, reactive oxygen species, and the vimentin network.
Li QF, Spinelli AM, Tang DD
American journal of physiology. Cell physiology297(2)C299-309(2009 Aug)

Vimentin regulates scribble activity by protecting it from proteasomal degradation.
Phua DC, Humbert PO, Hunziker W
Molecular biology of the cell20(12)2841-55(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knockdown of filamin A or vimentin in normal cells profoundly suppresses apical extrusion of the neighbouring transformed cells.
Title: Filamin acts as a key regulator in epithelial defence against transformed cells.

The development of a simple and rapid immunocytochemical technique for detection of cytokeratin, vimentin, and S-100 protein is reported.
Title: A simple and rapid immunocytochemical technique for detection of cytokeratin, vimentin, and S-100 protein in veterinary diagnostic cytology.

KRAP mediates InsP(3)R clustering in confluent MDCK cells and functions as an adapter, linking InsP(3)Rs to vimentin intermediate filaments.
Title: Vimentin and the K-Ras-induced actin-binding protein control inositol-(1,4,5)-trisphosphate receptor redistribution during MDCK cell differentiation.

Results suggest that Cdc42GAP regulates the vimentin network through the Cdc42-PAK pathway in smooth muscle cells during 5-HT stimulation.
Title: Cdc42GAP, reactive oxygen species, and the vimentin network.

The upregulation of vimentin expression during epithelial to mesenchymal transitions may stabilize Scrib to promote directed cell migration.
Title: Vimentin regulates scribble activity by protecting it from proteasomal degradation.

The following VIM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VIM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe33674	NM_001287023.1 Latest version!	Canis lupus familiaris vimentin (VIM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VIM ( NM_001287023.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001273952.1 Canis lupus familiaris vimentin (VIM), mRNA.

Price & Availability↑

CloneID	OCe33674
Clone ID Related Accession (Same CDS sequence)	NM_001287023.1
Accession Version	NM_001287023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Canis lupus familiaris(dog)
Product	vimentin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DN880362.1, DN433803.1, DN399845.1, DN371136.1, DN400894.1 and CO588619.1. On Nov 23, 2013 this sequence version replaced XM_851385.3. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCCACCA GGTCTGTGTC CTCGTCCTCC TACCGCAGGA TGTTCGGCGG CCCGGGCACC 
GGGAGCCGGC CGAGCTCCAC GCGGAGCTAC GTGACCACGT CCACCCGCAC CTACAGCCTG 
GGCAGCGCGC TGCGCCCCAG CACCAGCCGC AGCCTCTACG CCTCGTCCCC GGGCGGCGCG 
TACGCCACGC GCTCCTCCGC GGTGCGCCTG CGGAGCAGTG TGCCCGGCGT GCGTCTGCTG 
CAGGACTCGG TGGACTTCTC GCTGGCCGAC GCCATCAACA CCGAGTTCAA GAACACGCGC 
ACCAACGAGA AGGTGGAGCT GCAGGAGCTG AACGACCGCT TCGCCAACTA CATCGACAAG 
GTGCGCTTCC TGGAGCAGCA GAACAAGATC CTGCTGGCCG AGCTCGAGCA GCTCAAGGGC 
CAGGGCAAGT CGCGCCTGGG GGACCTCTAC GAGGAGGAGA TGCGGGAGCT GCGCCGGCAG 
GTGGACCAGC TCACCAACGA CAAGGCCCGC GTCGAGGTGG AGCGCGACAA CCTGGCCGAG 
GACATCATGC GGCTGCGGGA GAAGTTGCAA GAGGAGATGC TTCAGAGAGA GGAAGCCGAG 
AGCACCCTGC AGTCTTTCCG ACAGGATGTT GACAATGCTT CTTTGGCACG TCTTGACCTT 
GAGCGGAAAG TGGAATCCTT GCAAGAAGAG ATTGCCTTTT TGAAGAAACT ACATGATGAG 
GAAATCCAGG AGCTGCAGGC CCAGATTCAG GACCAGCATG TCCAAATCGA TATGGATGTT 
TCCAAGCCTG ACCTCACGGC TGCCCTGCGT GATGTACGCC AGCAATATGA AAGCGTGGCT 
GCCAAGAACC TTCAGGAGGC CGAGGAATGG TACAAGTCCA AGTTTGCCGA CCTCTCTGAG 
GCTGCTAACC GGAACAATGA TGCCCTGCGC CAGGCGAAGC AGGAGTCAAA TGAGTACCGG 
AGACAGGTGC AGTCCCTCAC CTGCGAAGTG GATGCACTCA AAGGGACTAA TGAGTCTCTG 
GAACGCCAGA TGCGTGAAAT GGAAGAGAAC TTTGCCGTCG AAGCTGCTAA CTACCAAGAC 
ACTATTGGCC GCCTGCAGGA TGAGATTCAG AACATGAAGG AAGAAATGGC TCGTCACCTT 
CGGGAGTACC AGGACCTGCT GAATGTTAAG ATGGCTCTCG ACATTGAGAT TGCCACCTAC 
AGGAAGCTGC TGGAAGGCGA GGAGAGCAGG ATTGCTCTGC CTCTTCCAAA CTTTTCTTCC 
CTGAACCTGA GGGAAACCAA CCTGGATTCA CTCCCTCTGG TTGACACTCA CTCAAAAAGA 
ACGCTTCTGA TTAAGACGGT TGAAACTAGG GATGGACAGG TTATCAACGA AACTTCTCAG 
CATCATGATG ACCTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273952.1
CDS	138..1538
Translation	MSTRSVSSSSYRRMFGGPGTGSRPSSTRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGAYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAESTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHDEEIQELQAQIQDQHVQIDMDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESNEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRIALPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE

Target ORF information:

RefSeq Version	NM_001287023.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris vimentin (VIM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287023.1

ORF Insert Sequence:

ATGTCCACCA GGTCTGTGTC CTCGTCCTCC TACCGCAGGA TGTTCGGCGG CCCGGGCACC 
GGGAGCCGGC CGAGCTCCAC GCGGAGCTAC GTGACCACGT CCACCCGCAC CTACAGCCTG 
GGCAGCGCGC TGCGCCCCAG CACCAGCCGC AGCCTCTACG CCTCGTCCCC GGGCGGCGCG 
TACGCCACGC GCTCCTCCGC GGTGCGCCTG CGGAGCAGTG TGCCCGGCGT GCGTCTGCTG 
CAGGACTCGG TGGACTTCTC GCTGGCCGAC GCCATCAACA CCGAGTTCAA GAACACGCGC 
ACCAACGAGA AGGTGGAGCT GCAGGAGCTG AACGACCGCT TCGCCAACTA CATCGACAAG 
GTGCGCTTCC TGGAGCAGCA GAACAAGATC CTGCTGGCCG AGCTCGAGCA GCTCAAGGGC 
CAGGGCAAGT CGCGCCTGGG GGACCTCTAC GAGGAGGAGA TGCGGGAGCT GCGCCGGCAG 
GTGGACCAGC TCACCAACGA CAAGGCCCGC GTCGAGGTGG AGCGCGACAA CCTGGCCGAG 
GACATCATGC GGCTGCGGGA GAAGTTGCAA GAGGAGATGC TTCAGAGAGA GGAAGCCGAG 
AGCACCCTGC AGTCTTTCCG ACAGGATGTT GACAATGCTT CTTTGGCACG TCTTGACCTT 
GAGCGGAAAG TGGAATCCTT GCAAGAAGAG ATTGCCTTTT TGAAGAAACT ACATGATGAG 
GAAATCCAGG AGCTGCAGGC CCAGATTCAG GACCAGCATG TCCAAATCGA TATGGATGTT 
TCCAAGCCTG ACCTCACGGC TGCCCTGCGT GATGTACGCC AGCAATATGA AAGCGTGGCT 
GCCAAGAACC TTCAGGAGGC CGAGGAATGG TACAAGTCCA AGTTTGCCGA CCTCTCTGAG 
GCTGCTAACC GGAACAATGA TGCCCTGCGC CAGGCGAAGC AGGAGTCAAA TGAGTACCGG 
AGACAGGTGC AGTCCCTCAC CTGCGAAGTG GATGCACTCA AAGGGACTAA TGAGTCTCTG 
GAACGCCAGA TGCGTGAAAT GGAAGAGAAC TTTGCCGTCG AAGCTGCTAA CTACCAAGAC 
ACTATTGGCC GCCTGCAGGA TGAGATTCAG AACATGAAGG AAGAAATGGC TCGTCACCTT 
CGGGAGTACC AGGACCTGCT GAATGTTAAG ATGGCTCTCG ACATTGAGAT TGCCACCTAC 
AGGAAGCTGC TGGAAGGCGA GGAGAGCAGG ATTGCTCTGC CTCTTCCAAA CTTTTCTTCC 
CTGAACCTGA GGGAAACCAA CCTGGATTCA CTCCCTCTGG TTGACACTCA CTCAAAAAGA 
ACGCTTCTGA TTAAGACGGT TGAAACTAGG GATGGACAGG TTATCAACGA AACTTCTCAG 
CATCATGATG ACCTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Filamin acts as a key regulator in epithelial defence against transformed cells.
Nature communications54428(2014 Jul)
Kajita M,Sugimura K,Ohoka A,Burden J,Suganuma H,Ikegawa M,Shimada T,Kitamura T,Shindoh M,Ishikawa S,Yamamoto S,Saitoh S,Yako Y,Takahashi R,Okajima T,Kikuta J,Maijima Y,Ishii M,Tada M,Fujita Y

A simple and rapid immunocytochemical technique for detection of cytokeratin, vimentin, and S-100 protein in veterinary diagnostic cytology.
Research in veterinary science93(3)1341-5(2012 Dec)
Sawa M,Yabuki A,Miyoshi N,Arai K,Yamato O

Vimentin and the K-Ras-induced actin-binding protein control inositol-(1,4,5)-trisphosphate receptor redistribution during MDCK cell differentiation.
Journal of cell science125(Pt 22)5428-40(2012 Nov)
Dingli F,Parys JB,Loew D,Saule S,Mery L

Cdc42GAP, reactive oxygen species, and the vimentin network.
American journal of physiology. Cell physiology297(2)C299-309(2009 Aug)
Li QF,Spinelli AM,Tang DD

Vimentin regulates scribble activity by protecting it from proteasomal degradation.
Molecular biology of the cell20(12)2841-55(2009 Jun)
Phua DC,Humbert PO,Hunziker W

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VIM cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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