Vim cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vim
Entrez Gene ID	81818
Full Name	vimentin
General protein information	Preferred Names vimentin Names vimentin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17q12.3
Summary	class-III intermediate filament of the cytoskeleton; along with desmin (the subunit specifically expressed in muscle), vimentin is specific for mesenchymal tissue [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031140.1	NP_112402.1	vimentin

Pathways from BioSystems

Homologies

Bos taurus (cattle)	VIM	NP_776394.2
Mus musculus (house mouse)	Vim	NP_035831.2
Danio rerio (zebrafish)	vim	NP_571947.1
Pan troglodytes (chimpanzee)	VIM	NP_001009148.1
Xenopus tropicalis (tropical clawed frog)	vim	NP_001128275.1
Canis lupus familiaris (dog)	VIM	XP_856478.1
Homo sapiens (human)	VIM	NP_003371.2
Rattus norvegicus (Norway rat)	Vim	NP_112402.1
Gallus gallus (chicken)	VIM	NP_001041541.1

Gene Ontology
Bibliography

Related articles in PubMed

Nerve Root Compression Increases Spinal Astrocytic Vimentin in Parallel With Sustained Pain and Endothelial Vimentin in Association With Spinal Vascular Reestablishment.
Smith JR, Lee J, Winkelstein BA
Spine42(19)1434-1439(2017 Oct)

Adolescent social isolation affects schizophrenia-like behavior and astrocyte biomarkers in the PFC of adult rats.
Sun L, Min L, Zhou H, Li M, Shao F, Wang W
Behavioural brain research333258-266(2017 Aug)

Electric field-induced suppression of PTEN drives epithelial-to-mesenchymal transition via mTORC1 activation.
Yan T, Jiang X, Guo X, Chen W, Tang D, Zhang J, Zhang X, Zhang D, Zhang Q, Jia J, Huang Y
Journal of dermatological science85(2)96-105(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

dephosphorylation of MAP2D at Thr256 and Thr259, phosphorylation of vimentin at Ser38 and Ser72, and the resulting enhanced binding of MAP2D to vimentin might contribute to the progesterone synthetic response required for ovulation.
Title: Dephosphorylation of MAP2D enhances its binding to vimentin in preovulatory ovarian granulosa cells.

considering the steroid hormone imbalance in late life period, as well as, the role of this imbalance in prostatic lesion development and progression, the aim of the study herein was to characterize and correlate prolactin, EGFR, alpha-actin and vimentin immunoreactivity in the prostatic epithelium and stroma of elderly rats, following steroid hormone imbalance.
Title: Prolactin, EGFR, vimentin and ?-actin profiles in elderly rat prostate subjected to steroid hormonal imbalance.

alpha-catulin has a unique function in co-localization with vimentin filaments that contributes to vascular endothelial cells migration via a pathway that may involve ROCK signaling.
Title: Alpha-Catulin Co-Localizes With Vimentin Intermediate Filaments and Functions in Pulmonary Vascular Endothelial Cell Migration via ROCK.

vimentin may be a novel ligand of IGF1R that promotes axonal growth in a similar manner to IGF1.
Title: Extracellular vimentin interacts with insulin-like growth factor 1 receptor to promote axonal growth.

In transected spinal cord rats, vimentin knockdown in the scar tissues showed a significant improvement on locomotor function in hindlimbs.
Title: Vimentin regulates neuroplasticity in transected spinal cord rats associated with micRNA138.

The following Vim gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vim cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07730	NM_031140.1 Latest version!	Rattus norvegicus vimentin (Vim), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$272.30 $389.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vim ( NM_031140.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112402.1 Rattus norvegicus vimentin (Vim), mRNA.

Price & Availability↑

CloneID	ORa07730
Clone ID Related Accession (Same CDS sequence)	NM_031140.1
Accession Version	NM_031140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	vimentin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X62952.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X62952.1, BC061847.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCCACCA GGTCCGTGTC CTCGTCCTCC TACCGCAGGA TGTTCGGTGG CTCCGGCACA 
TCGAGCCGGC CCAGCTCCAA CCGGAGCTAT GTGACCACAT CCACCCGCAC CTACAGCCTA 
GGCAGCGCGC TGCGCCCCAG CACTAGCCGC AGCCTCTATT CCTCGTCCCC GGGTGGCGCC 
TATGTGACCC GGTCCTCCGC CGTGCGCCTG CGGAGCAGCA TGCCCGGCGT GCGGCTGCTG 
CAGGACTCCG TGGACTTCTC GCTGGCCGAC GCCATCAACA CCGAGTTCAA GAACACCCGC 
ACCAACGAGA AGGTGGAATT GCAGGAGCTG AATGACCGCT TCGCCAACTA CATCGACAAG 
GTGCGCTTCC TCGAGCAGCA GAACAAAATC CTGCTGGCCG AGCTCGAGCA GCTTAAGGGC 
CAGGGCAAGT CGCGCCTGGG CGACCTCTAC GAGGAGGAGA TGAGGGAGTT GCGCCGGCAG 
GTGGATCAGC TCACCAATGA CAAGGCCCGT GTCGAGGTGG AGAGGGACAA CCTGGCCGAG 
GACATCATGC GGCTGCGAGA AAAATTGCAG GAGGAGATGC TCCAGAGGGA GGAAGCCGAG 
AGCACCCTGC AGTCATTCAG ACAGGATGTT GACAATGCTT CTCTGGCACG TCTTGACCTT 
GAACGTAAAG TGGAATCCTT GCAGGAAGAA ATTGCCTTTT TGAAGAAGCT GCACGATGAA 
GAGATCCAGG AGCTGCAGGC CCAGATTCAG GAACAGCATG TCCAGATCGA TGTGGACGTT 
TCCAAGCCTG ACCTCACCGC TGCCCTGCGT GATGTCCGCC AGCAGTATGA AAGTGTGGCT 
GCCAAGAACC TCCAGGAGGC CGAGGAATGG TACAAGTCCA AGTTTGCTGA CCTCTCTGAG 
GCTGCCAACC GGAACAACGA TGCCCTGCGC CAGGCAAAGC AGGAGTCAAA CGAATACCGG 
AGACAGGTGC AGTCACTCAC CTGCGAAGTG GATGCCCTTA AAGGCACTAA TGAGTCCCTG 
GAGCGCCAGA TGCGTGAAAT GGAAGAGAAT TTTGCCCTTG AAGCTGCTAA CTACCAGGAC 
ACTATTGGCC GCCTGCAGGA TGAGATCCAG AACATGAAGG AAGAGATGGC TCGCCACCTT 
CGTGAATACC AGGACCTGCT CAATGTAAAG ATGGCTCTTG ACATTGAGAT CGCCACCTAC 
AGGAAGCTGC TGGAAGGGGA GGAGAGCAGG ATTTCTCTGC CTCTTCCAAA CTTTTCTTCC 
CTGAACCTGA GAGAAACTAA CCTGGAGTCA CTTCCTCTGG TTGACACCCA CTCCAAAAGA 
ACACTCCTGA TTAAGACGGT TGAAACCAGA GACGGACAGG TGATCAATGA GACTTCTCAG 
CACCACGATG ACCTTGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112402.1
CDS	81..1481
Translation	MSTRSVSSSSYRRMFGGSGTSSRPSSNRSYVTTSTRTYSLGSALRPSTSRSLYSSSPGGAYVTRSSAVRLRSSMPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAESTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHDEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESNEYRRQVQSLTCEVDALKGTNESLERQMREMEENFALEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLESLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE

Target ORF information:

RefSeq Version	NM_031140.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus vimentin (Vim), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031140.1

ORF Insert Sequence:

ATGTCCACCA GGTCCGTGTC CTCGTCCTCC TACCGCAGGA TGTTCGGTGG CTCCGGCACA 
TCGAGCCGGC CCAGCTCCAA CCGGAGCTAT GTGACCACAT CCACCCGCAC CTACAGCCTA 
GGCAGCGCGC TGCGCCCCAG CACTAGCCGC AGCCTCTATT CCTCGTCCCC GGGTGGCGCC 
TATGTGACCC GGTCCTCCGC CGTGCGCCTG CGGAGCAGCA TGCCCGGCGT GCGGCTGCTG 
CAGGACTCCG TGGACTTCTC GCTGGCCGAC GCCATCAACA CCGAGTTCAA GAACACCCGC 
ACCAACGAGA AGGTGGAATT GCAGGAGCTG AATGACCGCT TCGCCAACTA CATCGACAAG 
GTGCGCTTCC TCGAGCAGCA GAACAAAATC CTGCTGGCCG AGCTCGAGCA GCTTAAGGGC 
CAGGGCAAGT CGCGCCTGGG CGACCTCTAC GAGGAGGAGA TGAGGGAGTT GCGCCGGCAG 
GTGGATCAGC TCACCAATGA CAAGGCCCGT GTCGAGGTGG AGAGGGACAA CCTGGCCGAG 
GACATCATGC GGCTGCGAGA AAAATTGCAG GAGGAGATGC TCCAGAGGGA GGAAGCCGAG 
AGCACCCTGC AGTCATTCAG ACAGGATGTT GACAATGCTT CTCTGGCACG TCTTGACCTT 
GAACGTAAAG TGGAATCCTT GCAGGAAGAA ATTGCCTTTT TGAAGAAGCT GCACGATGAA 
GAGATCCAGG AGCTGCAGGC CCAGATTCAG GAACAGCATG TCCAGATCGA TGTGGACGTT 
TCCAAGCCTG ACCTCACCGC TGCCCTGCGT GATGTCCGCC AGCAGTATGA AAGTGTGGCT 
GCCAAGAACC TCCAGGAGGC CGAGGAATGG TACAAGTCCA AGTTTGCTGA CCTCTCTGAG 
GCTGCCAACC GGAACAACGA TGCCCTGCGC CAGGCAAAGC AGGAGTCAAA CGAATACCGG 
AGACAGGTGC AGTCACTCAC CTGCGAAGTG GATGCCCTTA AAGGCACTAA TGAGTCCCTG 
GAGCGCCAGA TGCGTGAAAT GGAAGAGAAT TTTGCCCTTG AAGCTGCTAA CTACCAGGAC 
ACTATTGGCC GCCTGCAGGA TGAGATCCAG AACATGAAGG AAGAGATGGC TCGCCACCTT 
CGTGAATACC AGGACCTGCT CAATGTAAAG ATGGCTCTTG ACATTGAGAT CGCCACCTAC 
AGGAAGCTGC TGGAAGGGGA GGAGAGCAGG ATTTCTCTGC CTCTTCCAAA CTTTTCTTCC 
CTGAACCTGA GAGAAACTAA CCTGGAGTCA CTTCCTCTGG TTGACACCCA CTCCAAAAGA 
ACACTCCTGA TTAAGACGGT TGAAACCAGA GACGGACAGG TGATCAATGA GACTTCTCAG 
CACCACGATG ACCTTGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nerve Root Compression Increases Spinal Astrocytic Vimentin in Parallel With Sustained Pain and Endothelial Vimentin in Association With Spinal Vascular Reestablishment.
Spine42(19)1434-1439(2017 Oct)
Smith JR,Lee J,Winkelstein BA

Adolescent social isolation affects schizophrenia-like behavior and astrocyte biomarkers in the PFC of adult rats.
Behavioural brain research333258-266(2017 Aug)
Sun L,Min L,Zhou H,Li M,Shao F,Wang W

Electric field-induced suppression of PTEN drives epithelial-to-mesenchymal transition via mTORC1 activation.
Journal of dermatological science85(2)96-105(2017 Feb)
Yan T,Jiang X,Guo X,Chen W,Tang D,Zhang J,Zhang X,Zhang D,Zhang Q,Jia J,Huang Y

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Vim cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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