NFASC cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	NFASC
Entrez Gene ID	419824
Full Name	neurofascin
General protein information	Preferred Names neurofascin Names neurofascin
Gene Type	protein-coding
Organism	Gallus gallus(chicken)
Summary	This gene encodes a member of the L1 subgroup of the immunoglobulin superfamily. Members of the L1 subgroup are associated with axons during development of the nervous system, and are characterized by the presence in the extracellular portion of the protein of six immunoglobulin-like domains and five fibronectin type III-like repeats. This gene undergoes extensive alternative splicing. Additional variants have been described but are of lower abundance. [provided by RefSeq, May 2009].

mRNA and Protein(s)

mRNA	Protein	Name
XM_015298905.1	XP_015154391.1	neurofascin isoform X1
XM_015298908.1	XP_015154394.1	neurofascin isoform X4
XM_015298909.1	XP_015154395.1	neurofascin isoform X5
XM_015298916.1	XP_015154402.1	neurofascin isoform X12
XM_015298920.1	XP_015154406.1	neurofascin isoform X16
XM_015298903.2	XP_015154389.1	neurofascin isoform X1
XM_015298905.2	XP_015154391.1	neurofascin isoform X1
XM_015298908.2	XP_015154394.1	neurofascin isoform X4
XM_025143686.1	XP_024999454.1	neurofascin isoform X12
XM_015298919.2	XP_015154405.1	neurofascin isoform X18
NM_001160321.3	NP_001153793.1	neurofascin isoform 5 precursor
NM_001160322.3	NP_001153794.1	neurofascin isoform 6 precursor
NM_001160324.3	NP_001153796.1	neurofascin isoform 8 precursor
XM_015298914.1	XP_015154400.1	neurofascin isoform X10
XM_015298915.1	XP_015154401.1	neurofascin isoform X11
NM_001004709.4	NP_001004709.2	neurofascin isoform 3 precursor
XM_015298904.2	XP_015154390.1	neurofascin isoform X1
XM_015298906.2	XP_015154392.1	neurofascin isoform X2
XM_025143685.1	XP_024999453.1	neurofascin isoform X7
XM_015298913.2	XP_015154399.1	neurofascin isoform X10
XM_015298914.2	XP_015154400.1	neurofascin isoform X11
XM_015298915.2	XP_015154401.1	neurofascin isoform X13
XM_015298920.2	XP_015154406.1	neurofascin isoform X19
XM_015298904.1	XP_015154390.1	neurofascin isoform X1
XM_015298907.1	XP_015154393.1	neurofascin isoform X3
XM_015298910.1	XP_015154396.1	neurofascin isoform X6
XM_015298912.1	XP_015154398.1	neurofascin isoform X8
XM_015298917.1	XP_015154403.1	neurofascin isoform X13
XM_015298918.1	XP_015154404.1	neurofascin isoform X14
XM_015298919.1	XP_015154405.1	neurofascin isoform X15
NM_001160320.3	NP_001153792.1	neurofascin isoform 4 precursor
XM_015298907.2	XP_015154393.1	neurofascin isoform X3
XM_015298911.2	XP_015154397.1	neurofascin isoform X8
XM_015298917.2	XP_015154403.1	neurofascin isoform X16
XM_015298918.2	XP_015154404.1	neurofascin isoform X17
XM_015298906.1	XP_015154392.1	neurofascin isoform X2
XM_015298911.1	XP_015154397.1	neurofascin isoform X7
XM_015298913.1	XP_015154399.1	neurofascin isoform X9
XM_015298909.2	XP_015154395.1	neurofascin isoform X5
XM_015298916.2	XP_015154402.1	neurofascin isoform X15
XM_015298903.1	XP_015154389.1	neurofascin isoform X1
XM_015298910.2	XP_015154396.1	neurofascin isoform X6
XM_015298912.2	XP_015154398.1	neurofascin isoform X9
XM_025143688.1	XP_024999456.1	neurofascin isoform X14
NM_001160323.3	NP_001153795.1	neurofascin isoform 7 precursor
NM_001004493.4	NP_001004493.1	neurofascin isoform 2 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nfasc	NP_874385.1
Xenopus tropicalis (tropical clawed frog)	nfasc	XP_004910755.1
Rattus norvegicus (Norway rat)	Nfasc	NP_001153786.1
Danio rerio (zebrafish)	nfasca	NP_001139239.1
	NFASC	XP_001096628.1
Canis lupus familiaris (dog)	NFASC	XP_005640834.1
Pan troglodytes (chimpanzee)	NFASC	XP_003308755.1
Gallus gallus (chicken)	NFASC	NP_001153794.1
Homo sapiens (human)	NFASC	NP_001005388.2
Bos taurus (cattle)	NFASC	XP_005216764.1

Gene Ontology
Bibliography

Related articles in PubMed

Analysis of non-canonical fibroblast growth factor receptor 1 (FGFR1) interaction reveals regulatory and activating domains of neurofascin.
Kirschbaum K, Kriebel M, Kranz EU, P?tz O, Volkmer H
The Journal of biological chemistry284(42)28533-42(2009 Oct)

A regulated switch of chick neurofascin isoforms modulates ligand recognition and neurite extension.
Pruss T, Kranz EU, Niere M, Volkmer H
Molecular and cellular neurosciences31(2)354-65(2006 Feb)

Organization of the neurofascin gene and analysis of developmentally regulated alternative splicing.
Hassel B, Rathjen FG, Volkmer H
The Journal of biological chemistry272(45)28742-9(1997 Nov)

The L1 family of neural cell adhesion molecules: old proteins performing new tricks.
Hortsch M
Neuron17(4)587-93(1996 Oct)

Structure of the axonal surface recognition molecule neurofascin and its relationship to a neural subgroup of the immunoglobulin superfamily.
Volkmer H, Hassel B, Wolff JM, Frank R, Rathjen FG
The Journal of cell biology118(1)149-61(1992 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a neurofascin intracellular domain activates FGFR1 for neurite outgrowth, whereas the extracellular domain functions as an additional, regulatory FGFR1 interaction domain in the course of development
Title: Analysis of non-canonical fibroblast growth factor receptor 1 (FGFR1) interaction reveals regulatory and activating domains of neurofascin.

Results describe the roles of specific neurofascin isoforms in chick embryo dorsal root ganglion cells.
Title: A regulated switch of chick neurofascin isoforms modulates ligand recognition and neurite extension.

The following NFASC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NFASC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa46526	XM_015298919.2 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OGa147938	XM_025143685.1 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa147939	XM_025143686.1 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa147940	XM_025143688.1 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa46512	XM_015298903.2 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa46512	XM_015298904.2 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa46512	XM_015298905.2 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa46513	XM_015298906.2 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa46514	XM_015298907.2 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa46515	XM_015298908.2 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa46516	XM_015298909.2 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa46517	XM_015298910.2 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa46518	XM_015298911.2 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa46519	XM_015298912.2 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa46520	XM_015298913.2 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa46521	XM_015298914.2 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa46522	XM_015298915.2 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa46523	XM_015298916.2 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa46524	XM_015298917.2 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa46525	XM_015298918.2 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa46527	XM_015298920.2 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa28259	NM_001160320.3 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa28267	NM_001160322.3 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa28270	NM_001004493.4 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa28275	NM_001160321.3 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OGa28276	NM_001160323.3 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa28282	NM_001160324.3 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OGa28289	NM_001004709.4 Latest version!	Gallus gallus neurofascin (NFASC), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OGa46512	XM_015298905.1	Gallus gallus neurofascin (NFASC), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa46515	XM_015298908.1	Gallus gallus neurofascin (NFASC), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa46521	XM_015298914.1	Gallus gallus neurofascin (NFASC), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa46522	XM_015298915.1	Gallus gallus neurofascin (NFASC), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa46527	XM_015298920.1	Gallus gallus neurofascin (NFASC), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa46512	XM_015298904.1	Gallus gallus neurofascin (NFASC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa46526	XM_015298919.1	Gallus gallus neurofascin (NFASC), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OGa46514	XM_015298907.1	Gallus gallus neurofascin (NFASC), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa46524	XM_015298917.1	Gallus gallus neurofascin (NFASC), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa46525	XM_015298918.1	Gallus gallus neurofascin (NFASC), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa46513	XM_015298906.1	Gallus gallus neurofascin (NFASC), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa46518	XM_015298911.1	Gallus gallus neurofascin (NFASC), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa46520	XM_015298913.1	Gallus gallus neurofascin (NFASC), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa46516	XM_015298909.1	Gallus gallus neurofascin (NFASC), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa46523	XM_015298916.1	Gallus gallus neurofascin (NFASC), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa46512	XM_015298903.1	Gallus gallus neurofascin (NFASC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa46517	XM_015298910.1	Gallus gallus neurofascin (NFASC), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa46519	XM_015298912.1	Gallus gallus neurofascin (NFASC), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NFASC ( XM_015298919.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154405.1 Gallus gallus neurofascin (NFASC), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OGa46526
Clone ID Related Accession (Same CDS sequence)	XM_015298919.2 , XM_015298919.1
Accession Version	XM_015298919.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298919.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTCTTAC 
ACCAAGAACC ACGTGGACAT CGCGACCCAG GGCTGGTTCA TCGGGCTGAT GTGTGCCATC 
GCCCTCCTGG TCCTCATTCT GCTGATTGTT TGCTTCATTA AGAGGAGCAG AGGAGGGAAA 
TACCCAGTGC GTGACAACAA AGATGAGCAC CTGAATCCTG AAGACAAGAA CGTAGAAGAT 
GGCTCATTCG ACTACAGCGA TGAAGACAAC AAACCACTGC CCAACAGTCA GACCTCCCTG 
GATGGCACGA TAAAGCAACA GGAGAGTGAC GACAGCTTGG TGGACTACGG AGAGGGTGGG 
GAAGGGCAGT TCAACGAGGA CGGCTCCTTC ATTGGCCAGT ACACAGTGAA AAAGGACAAG 
GAGGAAACGG AAGGCAACGA GAGCTCAGAA GCCACGTCCC CAGTCAATGC TATCTACTCA 
TTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154405.1
CDS	169..3117
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDSYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYSDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298919.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298919.2

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTCTTAC 
ACCAAGAACC ACGTGGACAT CGCGACCCAG GGCTGGTTCA TCGGGCTGAT GTGTGCCATC 
GCCCTCCTGG TCCTCATTCT GCTGATTGTT TGCTTCATTA AGAGGAGCAG AGGAGGGAAA 
TACCCAGTGC GTGACAACAA AGATGAGCAC CTGAATCCTG AAGACAAGAA CGTAGAAGAT 
GGCTCATTCG ACTACAGCGA TGAAGACAAC AAACCACTGC CCAACAGTCA GACCTCCCTG 
GATGGCACGA TAAAGCAACA GGAGAGTGAC GACAGCTTGG TGGACTACGG AGAGGGTGGG 
GAAGGGCAGT TCAACGAGGA CGGCTCCTTC ATTGGCCAGT ACACAGTGAA AAAGGACAAG 
GAGGAAACGG AAGGCAACGA GAGCTCAGAA GCCACGTCCC CAGTCAATGC TATCTACTCA 
TTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298919.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154405.1 Gallus gallus neurofascin (NFASC), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OGa46526
Clone ID Related Accession (Same CDS sequence)	XM_015298919.2 , XM_015298919.1
Accession Version	XM_015298919.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTCTTAC 
ACCAAGAACC ACGTGGACAT CGCGACCCAG GGCTGGTTCA TCGGGCTGAT GTGTGCCATC 
GCCCTCCTGG TCCTCATTCT GCTGATTGTT TGCTTCATTA AGAGGAGCAG AGGAGGGAAA 
TACCCAGTGC GTGACAACAA AGATGAGCAC CTGAATCCTG AAGACAAGAA CGTAGAAGAT 
GGCTCATTCG ACTACAGCGA TGAAGACAAC AAACCACTGC CCAACAGTCA GACCTCCCTG 
GATGGCACGA TAAAGCAACA GGAGAGTGAC GACAGCTTGG TGGACTACGG AGAGGGTGGG 
GAAGGGCAGT TCAACGAGGA CGGCTCCTTC ATTGGCCAGT ACACAGTGAA AAAGGACAAG 
GAGGAAACGG AAGGCAACGA GAGCTCAGAA GCCACGTCCC CAGTCAATGC TATCTACTCA 
TTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154405.1
CDS	159..3107
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDSYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYSDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298919.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298919.1

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTCTTAC 
ACCAAGAACC ACGTGGACAT CGCGACCCAG GGCTGGTTCA TCGGGCTGAT GTGTGCCATC 
GCCCTCCTGG TCCTCATTCT GCTGATTGTT TGCTTCATTA AGAGGAGCAG AGGAGGGAAA 
TACCCAGTGC GTGACAACAA AGATGAGCAC CTGAATCCTG AAGACAAGAA CGTAGAAGAT 
GGCTCATTCG ACTACAGCGA TGAAGACAAC AAACCACTGC CCAACAGTCA GACCTCCCTG 
GATGGCACGA TAAAGCAACA GGAGAGTGAC GACAGCTTGG TGGACTACGG AGAGGGTGGG 
GAAGGGCAGT TCAACGAGGA CGGCTCCTTC ATTGGCCAGT ACACAGTGAA AAAGGACAAG 
GAGGAAACGG AAGGCAACGA GAGCTCAGAA GCCACGTCCC CAGTCAATGC TATCTACTCA 
TTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_025143685.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024999453.1 Gallus gallus neurofascin (NFASC), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OGa147938
Clone ID Related Accession (Same CDS sequence)	XM_025143685.1
Accession Version	XM_025143685.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG 
CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC 
CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA 
GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA 
AAACAGGAGC TGGCTTACAC CAAGAACCAC GTGGACATCG CGACCCAGGG CTGGTTCATC 
GGGCTGATGT GTGCCATCGC CCTCCTGGTC CTCATTCTGC TGATTGTTTG CTTCATTAAG 
AGGAGCAGAG GAGGGAAATA CCCAGTGCGT GACAACAAAG ATGAGCACCT GAATCCTGAA 
GACAAGAACG TAGAAGATGG CTCATTCGAC TACAGCGATG AAGACAACAA ACCACTGCCC 
AACAGTCAGA CCTCCCTGGA TGGCACGATA AAGCAACAGG AGAGTGACGA CAGCTTGGTG 
GACTACGGAG AGGGTGGGGA AGGGCAGTTC AACGAGGACG GCTCCTTCAT TGGCCAGTAC 
ACAGTGAAAA AGGACAAGGA GGAAACGGAA GGCAACGAGA GCTCAGAAGC CACGTCCCCA 
GTCAATGCTA TCTACTCATT AGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024999453.1
CDS	169..3975
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYSDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_025143685.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025143685.1

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG 
CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC 
CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA 
GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA 
AAACAGGAGC TGGCTTACAC CAAGAACCAC GTGGACATCG CGACCCAGGG CTGGTTCATC 
GGGCTGATGT GTGCCATCGC CCTCCTGGTC CTCATTCTGC TGATTGTTTG CTTCATTAAG 
AGGAGCAGAG GAGGGAAATA CCCAGTGCGT GACAACAAAG ATGAGCACCT GAATCCTGAA 
GACAAGAACG TAGAAGATGG CTCATTCGAC TACAGCGATG AAGACAACAA ACCACTGCCC 
AACAGTCAGA CCTCCCTGGA TGGCACGATA AAGCAACAGG AGAGTGACGA CAGCTTGGTG 
GACTACGGAG AGGGTGGGGA AGGGCAGTTC AACGAGGACG GCTCCTTCAT TGGCCAGTAC 
ACAGTGAAAA AGGACAAGGA GGAAACGGAA GGCAACGAGA GCTCAGAAGC CACGTCCCCA 
GTCAATGCTA TCTACTCATT AGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_025143686.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024999454.1 Gallus gallus neurofascin (NFASC), transcript variant X14, mRNA.

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CloneID	OGa147939
Clone ID Related Accession (Same CDS sequence)	XM_025143686.1
Accession Version	XM_025143686.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCTTACAC CAAGAACCAC GTGGACATCG CGACCCAGGG CTGGTTCATC 
GGGCTGATGT GTGCCATCGC CCTCCTGGTC CTCATTCTGC TGATTGTTTG CTTCATTAAG 
AGGAGCAGAG GAGGGAAATA CCCAGTGCGT GACAACAAAG ATGAGCACCT GAATCCTGAA 
GACAAGAACG TAGAAGATGG CTCATTCGAC TACAGCGATG AAGACAACAA ACCACTGCCC 
AACAGTCAGA CCTCCCTGGA TGGCACGATA AAGCAACAGG AGAGTGACGA CAGCTTGGTG 
GACTACGGAG AGGGTGGGGA AGGGCAGTTC AACGAGGACG GCTCCTTCAT TGGCCAGTAC 
ACAGTGAAAA AGGACAAGGA GGAAACGGAA GGCAACGAGA GCTCAGAAGC CACGTCCCCA 
GTCAATGCTA TCTACTCATT AGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024999454.1
CDS	169..3735
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYSDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_025143686.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025143686.1

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCTTACAC CAAGAACCAC GTGGACATCG CGACCCAGGG CTGGTTCATC 
GGGCTGATGT GTGCCATCGC CCTCCTGGTC CTCATTCTGC TGATTGTTTG CTTCATTAAG 
AGGAGCAGAG GAGGGAAATA CCCAGTGCGT GACAACAAAG ATGAGCACCT GAATCCTGAA 
GACAAGAACG TAGAAGATGG CTCATTCGAC TACAGCGATG AAGACAACAA ACCACTGCCC 
AACAGTCAGA CCTCCCTGGA TGGCACGATA AAGCAACAGG AGAGTGACGA CAGCTTGGTG 
GACTACGGAG AGGGTGGGGA AGGGCAGTTC AACGAGGACG GCTCCTTCAT TGGCCAGTAC 
ACAGTGAAAA AGGACAAGGA GGAAACGGAA GGCAACGAGA GCTCAGAAGC CACGTCCCCA 
GTCAATGCTA TCTACTCATT AGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_025143688.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024999456.1 Gallus gallus neurofascin (NFASC), transcript variant X17, mRNA.

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CloneID	OGa147940
Clone ID Related Accession (Same CDS sequence)	XM_025143688.1
Accession Version	XM_025143688.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGAGCGA CCCGACCGGC CCCGGGACCT GGAGCTGTCA 
GACCTGGCTG AGAGGAGCGT GAAGCTGACA TGGATTCCTG GCGATGACAA CAACAGCCCC 
ATCACAGACT ACATCGTCCA GTTTGAGGAG GACCGCTTCC AGCCTGGCAC GTGGCACAAC 
CACTCCAGGT ATCCTGGGAA TGTCAACTCG GCCCTCCTGA GCCTCTCTCC TTACGTCAAC 
TACCAATTTC GGGTGATTGC AGTGAACGAC GTGGGCAGCA GCCTGCCCAG CATGCCCTCA 
GAACGATACC AGACCAGCGG GGCACGACCT GAAATTAACC CAACAGGAGT TCAAGGAGCA 
GGGACCCAAA AAAACAACAT GGAGATAACC TGGACGCCTC TGAATGCAAC TCAAGCCTAT 
GGGCCCAACC TCCGTTACAT CGTGCGGTGG AGGCGAAGGG ACCCACGTGG GAGTTGGTAC 
AACGAGACGG TGAAGGCACC ACGACACGTC GTCTGGAACA CACCCATCTA CGTCCCCTAC 
GAGATCAAAG TGCAGGCAGA GAATGACTTT GGTAGAGCTC CAGAGCCTGA GACCTACATC 
GGCTACTCAG GGGAAGATTA TCCCAAGGCT GCACCTACAG ATGTTAGGAT AAGAGTTTTA 
AACAGCACTG CCATTGCTCT GACATGGACC CGCGTGCACC TGGACACCAT CCAGGGACAG 
CTTAAGGAGT ACAGAGCCTA TTTCTGGAGA GACAGTAGTT TGCTGAAGAA CCTGTGGGTC 
TCCAAAAAAC GGCAGTATGT GAGTTTTCCT GGAGACCGAA ACCGGGGCAT AGTGTCCCGG 
CTGTTCCCTT ACAGCAACTA CAAGCTTGAG ATGGTTGTAA CCAACGGGAG AGGCGATGGG 
CCCCGCAGCG AAGTCAAGGA GTTCCCCACA CCTGAAGGAG TGCCCAGCTC CCCCAGGTAC 
TTAAGAATCC GACAGCCAAA TCTGGAAAGC ATCAATTTGG AGTGGGATCA CCCAGAACAT 
CCCAATGGAG TCCTCACGGG ATACAACCTT AGATATCAAG CCTTTAACGG ATCCAAAACG 
GGCAGAACCC TGGTAGAGAA CTTCTCTCCC AACCAGACAA GGTTCACCGT GCAGAGGACA 
GACCCCATCT CGCGCTATCG ATTCTTCCTG CGTGCTCGGA CACAGGTGGG AGATGGAGAA 
GTCATAGTGG AAGAGTCACC AGCGCTCCTG AATGAAGCTT ACACCAAGAA CCACGTGGAC 
ATCGCGACCC AGGGCTGGTT CATCGGGCTG ATGTGTGCCA TCGCCCTCCT GGTCCTCATT 
CTGCTGATTG TTTGCTTCAT TAAGAGGAGC AGAGGAGGGA AATACCCAGT GCGTGACAAC 
AAAGATGAGC ACCTGAATCC TGAAGACAAG AACGTAGAAG ATGGCTCATT CGACTACAGG 
TCTCTTGAAA GCGATGAAGA CAACAAACCA CTGCCCAACA GTCAGACCTC CCTGGATGGC 
ACGATAAAGC AACAGGAGAG TGACGACAGC TTGGTGGACT ACGGAGAGGG TGGGGAAGGG 
CAGTTCAACG AGGACGGCTC CTTCATTGGC CAGTACACAG TGAAAAAGGA CAAGGAGGAA 
ACGGAAGGCA ACGAGAGCTC AGAAGCCACG TCCCCAGTCA ATGCTATCTA CTCATTAGCG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024999456.1
CDS	169..3711
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_025143688.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025143688.1

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGAGCGA CCCGACCGGC CCCGGGACCT GGAGCTGTCA 
GACCTGGCTG AGAGGAGCGT GAAGCTGACA TGGATTCCTG GCGATGACAA CAACAGCCCC 
ATCACAGACT ACATCGTCCA GTTTGAGGAG GACCGCTTCC AGCCTGGCAC GTGGCACAAC 
CACTCCAGGT ATCCTGGGAA TGTCAACTCG GCCCTCCTGA GCCTCTCTCC TTACGTCAAC 
TACCAATTTC GGGTGATTGC AGTGAACGAC GTGGGCAGCA GCCTGCCCAG CATGCCCTCA 
GAACGATACC AGACCAGCGG GGCACGACCT GAAATTAACC CAACAGGAGT TCAAGGAGCA 
GGGACCCAAA AAAACAACAT GGAGATAACC TGGACGCCTC TGAATGCAAC TCAAGCCTAT 
GGGCCCAACC TCCGTTACAT CGTGCGGTGG AGGCGAAGGG ACCCACGTGG GAGTTGGTAC 
AACGAGACGG TGAAGGCACC ACGACACGTC GTCTGGAACA CACCCATCTA CGTCCCCTAC 
GAGATCAAAG TGCAGGCAGA GAATGACTTT GGTAGAGCTC CAGAGCCTGA GACCTACATC 
GGCTACTCAG GGGAAGATTA TCCCAAGGCT GCACCTACAG ATGTTAGGAT AAGAGTTTTA 
AACAGCACTG CCATTGCTCT GACATGGACC CGCGTGCACC TGGACACCAT CCAGGGACAG 
CTTAAGGAGT ACAGAGCCTA TTTCTGGAGA GACAGTAGTT TGCTGAAGAA CCTGTGGGTC 
TCCAAAAAAC GGCAGTATGT GAGTTTTCCT GGAGACCGAA ACCGGGGCAT AGTGTCCCGG 
CTGTTCCCTT ACAGCAACTA CAAGCTTGAG ATGGTTGTAA CCAACGGGAG AGGCGATGGG 
CCCCGCAGCG AAGTCAAGGA GTTCCCCACA CCTGAAGGAG TGCCCAGCTC CCCCAGGTAC 
TTAAGAATCC GACAGCCAAA TCTGGAAAGC ATCAATTTGG AGTGGGATCA CCCAGAACAT 
CCCAATGGAG TCCTCACGGG ATACAACCTT AGATATCAAG CCTTTAACGG ATCCAAAACG 
GGCAGAACCC TGGTAGAGAA CTTCTCTCCC AACCAGACAA GGTTCACCGT GCAGAGGACA 
GACCCCATCT CGCGCTATCG ATTCTTCCTG CGTGCTCGGA CACAGGTGGG AGATGGAGAA 
GTCATAGTGG AAGAGTCACC AGCGCTCCTG AATGAAGCTT ACACCAAGAA CCACGTGGAC 
ATCGCGACCC AGGGCTGGTT CATCGGGCTG ATGTGTGCCA TCGCCCTCCT GGTCCTCATT 
CTGCTGATTG TTTGCTTCAT TAAGAGGAGC AGAGGAGGGA AATACCCAGT GCGTGACAAC 
AAAGATGAGC ACCTGAATCC TGAAGACAAG AACGTAGAAG ATGGCTCATT CGACTACAGG 
TCTCTTGAAA GCGATGAAGA CAACAAACCA CTGCCCAACA GTCAGACCTC CCTGGATGGC 
ACGATAAAGC AACAGGAGAG TGACGACAGC TTGGTGGACT ACGGAGAGGG TGGGGAAGGG 
CAGTTCAACG AGGACGGCTC CTTCATTGGC CAGTACACAG TGAAAAAGGA CAAGGAGGAA 
ACGGAAGGCA ACGAGAGCTC AGAAGCCACG TCCCCAGTCA ATGCTATCTA CTCATTAGCG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298905.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154391.1 Gallus gallus neurofascin (NFASC), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa46512
Clone ID Related Accession (Same CDS sequence)	XM_015298905.1 , XM_015298903.2 , XM_015298905.2 , XM_015298904.2 , XM_015298904.1 , XM_015298903.1
Accession Version	XM_015298905.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG 
CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC 
CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA 
GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA 
AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT 
AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG 
TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT 
GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG 
TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC 
CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG 
GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG 
CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC 
GACTACAGGT CTCTTGAAAG CGATGAAGAC AACAAACCAC TGCCCAACAG TCAGACCTCC 
CTGGATGGCA CGATAAAGCA ACAGGAGAGT GACGACAGCT TGGTGGACTA CGGAGAGGGT 
GGGGAAGGGC AGTTCAACGA GGACGGCTCC TTCATTGGCC AGTACACAGT GAAAAAGGAC 
AAGGAGGAAA CGGAAGGCAA CGAGAGCTCA GAAGCCACGT CCCCAGTCAA TGCTATCTAC 
TCATTAGCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154391.1
CDS	95..4186
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELATNGSSIWDIRAWANSNWANITWSHNYSAGTDFVVKYITSNKTEKSIPVKAQTPSSVQLANLTPGMVYKLWVFPIWSSPSEHSYITFTTSSAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298905.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298905.1

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG 
CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC 
CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA 
GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA 
AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT 
AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG 
TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT 
GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG 
TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC 
CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG 
GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG 
CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC 
GACTACAGGT CTCTTGAAAG CGATGAAGAC AACAAACCAC TGCCCAACAG TCAGACCTCC 
CTGGATGGCA CGATAAAGCA ACAGGAGAGT GACGACAGCT TGGTGGACTA CGGAGAGGGT 
GGGGAAGGGC AGTTCAACGA GGACGGCTCC TTCATTGGCC AGTACACAGT GAAAAAGGAC 
AAGGAGGAAA CGGAAGGCAA CGAGAGCTCA GAAGCCACGT CCCCAGTCAA TGCTATCTAC 
TCATTAGCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298903.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154389.1 Gallus gallus neurofascin (NFASC), transcript variant X1, mRNA.

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CloneID	OGa46512
Clone ID Related Accession (Same CDS sequence)	XM_015298905.1 , XM_015298903.2 , XM_015298905.2 , XM_015298904.2 , XM_015298904.1 , XM_015298903.1
Accession Version	XM_015298903.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298903.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG 
CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC 
CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA 
GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA 
AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT 
AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG 
TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT 
GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG 
TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC 
CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG 
GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG 
CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC 
GACTACAGGT CTCTTGAAAG CGATGAAGAC AACAAACCAC TGCCCAACAG TCAGACCTCC 
CTGGATGGCA CGATAAAGCA ACAGGAGAGT GACGACAGCT TGGTGGACTA CGGAGAGGGT 
GGGGAAGGGC AGTTCAACGA GGACGGCTCC TTCATTGGCC AGTACACAGT GAAAAAGGAC 
AAGGAGGAAA CGGAAGGCAA CGAGAGCTCA GAAGCCACGT CCCCAGTCAA TGCTATCTAC 
TCATTAGCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154389.1
CDS	169..4260
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELATNGSSIWDIRAWANSNWANITWSHNYSAGTDFVVKYITSNKTEKSIPVKAQTPSSVQLANLTPGMVYKLWVFPIWSSPSEHSYITFTTSSAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298903.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298903.2

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG 
CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC 
CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA 
GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA 
AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT 
AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG 
TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT 
GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG 
TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC 
CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG 
GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG 
CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC 
GACTACAGGT CTCTTGAAAG CGATGAAGAC AACAAACCAC TGCCCAACAG TCAGACCTCC 
CTGGATGGCA CGATAAAGCA ACAGGAGAGT GACGACAGCT TGGTGGACTA CGGAGAGGGT 
GGGGAAGGGC AGTTCAACGA GGACGGCTCC TTCATTGGCC AGTACACAGT GAAAAAGGAC 
AAGGAGGAAA CGGAAGGCAA CGAGAGCTCA GAAGCCACGT CCCCAGTCAA TGCTATCTAC 
TCATTAGCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298905.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154391.1 Gallus gallus neurofascin (NFASC), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa46512
Clone ID Related Accession (Same CDS sequence)	XM_015298905.1 , XM_015298903.2 , XM_015298905.2 , XM_015298904.2 , XM_015298904.1 , XM_015298903.1
Accession Version	XM_015298905.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298905.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG 
CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC 
CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA 
GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA 
AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT 
AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG 
TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT 
GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG 
TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC 
CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG 
GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG 
CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC 
GACTACAGGT CTCTTGAAAG CGATGAAGAC AACAAACCAC TGCCCAACAG TCAGACCTCC 
CTGGATGGCA CGATAAAGCA ACAGGAGAGT GACGACAGCT TGGTGGACTA CGGAGAGGGT 
GGGGAAGGGC AGTTCAACGA GGACGGCTCC TTCATTGGCC AGTACACAGT GAAAAAGGAC 
AAGGAGGAAA CGGAAGGCAA CGAGAGCTCA GAAGCCACGT CCCCAGTCAA TGCTATCTAC 
TCATTAGCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154391.1
CDS	91..4182
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELATNGSSIWDIRAWANSNWANITWSHNYSAGTDFVVKYITSNKTEKSIPVKAQTPSSVQLANLTPGMVYKLWVFPIWSSPSEHSYITFTTSSAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298905.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298905.2

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG 
CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC 
CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA 
GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA 
AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT 
AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG 
TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT 
GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG 
TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC 
CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG 
GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG 
CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC 
GACTACAGGT CTCTTGAAAG CGATGAAGAC AACAAACCAC TGCCCAACAG TCAGACCTCC 
CTGGATGGCA CGATAAAGCA ACAGGAGAGT GACGACAGCT TGGTGGACTA CGGAGAGGGT 
GGGGAAGGGC AGTTCAACGA GGACGGCTCC TTCATTGGCC AGTACACAGT GAAAAAGGAC 
AAGGAGGAAA CGGAAGGCAA CGAGAGCTCA GAAGCCACGT CCCCAGTCAA TGCTATCTAC 
TCATTAGCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298904.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154390.1 Gallus gallus neurofascin (NFASC), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa46512
Clone ID Related Accession (Same CDS sequence)	XM_015298905.1 , XM_015298903.2 , XM_015298905.2 , XM_015298904.2 , XM_015298904.1 , XM_015298903.1
Accession Version	XM_015298904.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298904.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG 
CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC 
CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA 
GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA 
AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT 
AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG 
TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT 
GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG 
TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC 
CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG 
GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG 
CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC 
GACTACAGGT CTCTTGAAAG CGATGAAGAC AACAAACCAC TGCCCAACAG TCAGACCTCC 
CTGGATGGCA CGATAAAGCA ACAGGAGAGT GACGACAGCT TGGTGGACTA CGGAGAGGGT 
GGGGAAGGGC AGTTCAACGA GGACGGCTCC TTCATTGGCC AGTACACAGT GAAAAAGGAC 
AAGGAGGAAA CGGAAGGCAA CGAGAGCTCA GAAGCCACGT CCCCAGTCAA TGCTATCTAC 
TCATTAGCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154390.1
CDS	354..4445
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELATNGSSIWDIRAWANSNWANITWSHNYSAGTDFVVKYITSNKTEKSIPVKAQTPSSVQLANLTPGMVYKLWVFPIWSSPSEHSYITFTTSSAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298904.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298904.2

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG 
CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC 
CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA 
GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA 
AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT 
AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG 
TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT 
GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG 
TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC 
CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG 
GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG 
CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC 
GACTACAGGT CTCTTGAAAG CGATGAAGAC AACAAACCAC TGCCCAACAG TCAGACCTCC 
CTGGATGGCA CGATAAAGCA ACAGGAGAGT GACGACAGCT TGGTGGACTA CGGAGAGGGT 
GGGGAAGGGC AGTTCAACGA GGACGGCTCC TTCATTGGCC AGTACACAGT GAAAAAGGAC 
AAGGAGGAAA CGGAAGGCAA CGAGAGCTCA GAAGCCACGT CCCCAGTCAA TGCTATCTAC 
TCATTAGCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298904.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154390.1 Gallus gallus neurofascin (NFASC), transcript variant X2, mRNA.

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CloneID	OGa46512
Clone ID Related Accession (Same CDS sequence)	XM_015298905.1 , XM_015298903.2 , XM_015298905.2 , XM_015298904.2 , XM_015298904.1 , XM_015298903.1
Accession Version	XM_015298904.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG 
CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC 
CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA 
GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA 
AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT 
AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG 
TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT 
GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG 
TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC 
CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG 
GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG 
CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC 
GACTACAGGT CTCTTGAAAG CGATGAAGAC AACAAACCAC TGCCCAACAG TCAGACCTCC 
CTGGATGGCA CGATAAAGCA ACAGGAGAGT GACGACAGCT TGGTGGACTA CGGAGAGGGT 
GGGGAAGGGC AGTTCAACGA GGACGGCTCC TTCATTGGCC AGTACACAGT GAAAAAGGAC 
AAGGAGGAAA CGGAAGGCAA CGAGAGCTCA GAAGCCACGT CCCCAGTCAA TGCTATCTAC 
TCATTAGCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154390.1
CDS	367..4458
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELATNGSSIWDIRAWANSNWANITWSHNYSAGTDFVVKYITSNKTEKSIPVKAQTPSSVQLANLTPGMVYKLWVFPIWSSPSEHSYITFTTSSAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298904.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298904.1

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG 
CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC 
CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA 
GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA 
AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT 
AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG 
TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT 
GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG 
TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC 
CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG 
GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG 
CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC 
GACTACAGGT CTCTTGAAAG CGATGAAGAC AACAAACCAC TGCCCAACAG TCAGACCTCC 
CTGGATGGCA CGATAAAGCA ACAGGAGAGT GACGACAGCT TGGTGGACTA CGGAGAGGGT 
GGGGAAGGGC AGTTCAACGA GGACGGCTCC TTCATTGGCC AGTACACAGT GAAAAAGGAC 
AAGGAGGAAA CGGAAGGCAA CGAGAGCTCA GAAGCCACGT CCCCAGTCAA TGCTATCTAC 
TCATTAGCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298903.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154389.1 Gallus gallus neurofascin (NFASC), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa46512
Clone ID Related Accession (Same CDS sequence)	XM_015298905.1 , XM_015298903.2 , XM_015298905.2 , XM_015298904.2 , XM_015298904.1 , XM_015298903.1
Accession Version	XM_015298903.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG 
CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC 
CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA 
GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA 
AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT 
AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG 
TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT 
GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG 
TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC 
CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG 
GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG 
CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC 
GACTACAGGT CTCTTGAAAG CGATGAAGAC AACAAACCAC TGCCCAACAG TCAGACCTCC 
CTGGATGGCA CGATAAAGCA ACAGGAGAGT GACGACAGCT TGGTGGACTA CGGAGAGGGT 
GGGGAAGGGC AGTTCAACGA GGACGGCTCC TTCATTGGCC AGTACACAGT GAAAAAGGAC 
AAGGAGGAAA CGGAAGGCAA CGAGAGCTCA GAAGCCACGT CCCCAGTCAA TGCTATCTAC 
TCATTAGCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154389.1
CDS	159..4250
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELATNGSSIWDIRAWANSNWANITWSHNYSAGTDFVVKYITSNKTEKSIPVKAQTPSSVQLANLTPGMVYKLWVFPIWSSPSEHSYITFTTSSAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298903.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298903.1

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG 
CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC 
CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA 
GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA 
AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT 
AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG 
TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT 
GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG 
TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC 
CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG 
GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG 
CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC 
GACTACAGGT CTCTTGAAAG CGATGAAGAC AACAAACCAC TGCCCAACAG TCAGACCTCC 
CTGGATGGCA CGATAAAGCA ACAGGAGAGT GACGACAGCT TGGTGGACTA CGGAGAGGGT 
GGGGAAGGGC AGTTCAACGA GGACGGCTCC TTCATTGGCC AGTACACAGT GAAAAAGGAC 
AAGGAGGAAA CGGAAGGCAA CGAGAGCTCA GAAGCCACGT CCCCAGTCAA TGCTATCTAC 
TCATTAGCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298906.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154392.1 Gallus gallus neurofascin (NFASC), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa46513
Clone ID Related Accession (Same CDS sequence)	XM_015298906.2 , XM_015298906.1
Accession Version	XM_015298906.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298906.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG 
CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC 
CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA 
GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA 
AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT 
AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG 
TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT 
GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG 
TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC 
CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG 
GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG 
CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC 
GACTACAGCG ATGAAGACAA CAAACCACTG CCCAACAGTC AGACCTCCCT GGATGGCACG 
ATAAAGCAAC AGGAGAGTGA CGACAGCTTG GTGGACTACG GAGAGGGTGG GGAAGGGCAG 
TTCAACGAGG ACGGCTCCTT CATTGGCCAG TACACAGTGA AAAAGGACAA GGAGGAAACG 
GAAGGCAACG AGAGCTCAGA AGCCACGTCC CCAGTCAATG CTATCTACTC ATTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154392.1
CDS	169..4248
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELATNGSSIWDIRAWANSNWANITWSHNYSAGTDFVVKYITSNKTEKSIPVKAQTPSSVQLANLTPGMVYKLWVFPIWSSPSEHSYITFTTSSAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYSDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298906.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298906.2

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG 
CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC 
CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA 
GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA 
AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT 
AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG 
TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT 
GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG 
TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC 
CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG 
GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG 
CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC 
GACTACAGCG ATGAAGACAA CAAACCACTG CCCAACAGTC AGACCTCCCT GGATGGCACG 
ATAAAGCAAC AGGAGAGTGA CGACAGCTTG GTGGACTACG GAGAGGGTGG GGAAGGGCAG 
TTCAACGAGG ACGGCTCCTT CATTGGCCAG TACACAGTGA AAAAGGACAA GGAGGAAACG 
GAAGGCAACG AGAGCTCAGA AGCCACGTCC CCAGTCAATG CTATCTACTC ATTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298906.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154392.1 Gallus gallus neurofascin (NFASC), transcript variant X4, mRNA.

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CloneID	OGa46513
Clone ID Related Accession (Same CDS sequence)	XM_015298906.2 , XM_015298906.1
Accession Version	XM_015298906.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG 
CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC 
CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA 
GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA 
AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT 
AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG 
TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT 
GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG 
TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC 
CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG 
GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG 
CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC 
GACTACAGCG ATGAAGACAA CAAACCACTG CCCAACAGTC AGACCTCCCT GGATGGCACG 
ATAAAGCAAC AGGAGAGTGA CGACAGCTTG GTGGACTACG GAGAGGGTGG GGAAGGGCAG 
TTCAACGAGG ACGGCTCCTT CATTGGCCAG TACACAGTGA AAAAGGACAA GGAGGAAACG 
GAAGGCAACG AGAGCTCAGA AGCCACGTCC CCAGTCAATG CTATCTACTC ATTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154392.1
CDS	159..4238
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELATNGSSIWDIRAWANSNWANITWSHNYSAGTDFVVKYITSNKTEKSIPVKAQTPSSVQLANLTPGMVYKLWVFPIWSSPSEHSYITFTTSSAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYSDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298906.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298906.1

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG 
CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC 
CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA 
GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA 
AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT 
AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG 
TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT 
GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG 
TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC 
CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG 
GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG 
CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC 
GACTACAGCG ATGAAGACAA CAAACCACTG CCCAACAGTC AGACCTCCCT GGATGGCACG 
ATAAAGCAAC AGGAGAGTGA CGACAGCTTG GTGGACTACG GAGAGGGTGG GGAAGGGCAG 
TTCAACGAGG ACGGCTCCTT CATTGGCCAG TACACAGTGA AAAAGGACAA GGAGGAAACG 
GAAGGCAACG AGAGCTCAGA AGCCACGTCC CCAGTCAATG CTATCTACTC ATTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298907.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154393.1 Gallus gallus neurofascin (NFASC), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OGa46514
Clone ID Related Accession (Same CDS sequence)	XM_015298907.1 , XM_015298907.2
Accession Version	XM_015298907.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG 
CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC 
AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA 
AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG 
GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC 
ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG 
AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT 
CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG 
GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG 
GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG 
AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA 
AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC 
CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC 
ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA 
GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG 
GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG 
ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG 
GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC 
CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG 
GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC 
AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG 
CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC 
CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC 
CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG 
AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC 
AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC 
GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC 
CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC 
AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT 
GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC 
GTGCTAGCAA TCCCTGCTAA CCGTTTGAGA GACTTACCTA AAGAGCGACC CGACCGGCCC 
CGGGACCTGG AGCTGTCAGA CCTGGCTGAG AGGAGCGTGA AGCTGACATG GATTCCTGGC 
GATGACAACA ACAGCCCCAT CACAGACTAC ATCGTCCAGT TTGAGGAGGA CCGCTTCCAG 
CCTGGCACGT GGCACAACCA CTCCAGGTAT CCTGGGAATG TCAACTCGGC CCTCCTGAGC 
CTCTCTCCTT ACGTCAACTA CCAATTTCGG GTGATTGCAG TGAACGACGT GGGCAGCAGC 
CTGCCCAGCA TGCCCTCAGA ACGATACCAG ACCAGCGGGG CACGACCTGA AATTAACCCA 
ACAGGAGTTC AAGGAGCAGG GACCCAAAAA AACAACATGG AGATAACCTG GACGCCTCTG 
AATGCAACTC AAGCCTATGG GCCCAACCTC CGTTACATCG TGCGGTGGAG GCGAAGGGAC 
CCACGTGGGA GTTGGTACAA CGAGACGGTG AAGGCACCAC GACACGTCGT CTGGAACACA 
CCCATCTACG TCCCCTACGA GATCAAAGTG CAGGCAGAGA ATGACTTTGG TAGAGCTCCA 
GAGCCTGAGA CCTACATCGG CTACTCAGGG GAAGATTATC CCAAGGCTGC ACCTACAGAT 
GTTAGGATAA GAGTTTTAAA CAGCACTGCC ATTGCTCTGA CATGGACCCG CGTGCACCTG 
GACACCATCC AGGGACAGCT TAAGGAGTAC AGAGCCTATT TCTGGAGAGA CAGTAGTTTG 
CTGAAGAACC TGTGGGTCTC CAAAAAACGG CAGTATGTGA GTTTTCCTGG AGACCGAAAC 
CGGGGCATAG TGTCCCGGCT GTTCCCTTAC AGCAACTACA AGCTTGAGAT GGTTGTAACC 
AACGGGAGAG GCGATGGGCC CCGCAGCGAA GTCAAGGAGT TCCCCACACC TGAAGGAGTG 
CCCAGCTCCC CCAGGTACTT AAGAATCCGA CAGCCAAATC TGGAAAGCAT CAATTTGGAG 
TGGGATCACC CAGAACATCC CAATGGAGTC CTCACGGGAT ACAACCTTAG ATATCAAGCC 
TTTAACGGAT CCAAAACGGG CAGAACCCTG GTAGAGAACT TCTCTCCCAA CCAGACAAGG 
TTCACCGTGC AGAGGACAGA CCCCATCTCG CGCTATCGAT TCTTCCTGCG TGCTCGGACA 
CAGGTGGGAG ATGGAGAAGT CATAGTGGAA GAGTCACCAG CGCTCCTGAA TGAAGCCACG 
CCAACCCCAG CCTCCACCTG GTTGCCTCCA CCTACAACCG AGCTAACTCC AGCAGCCACC 
ATTGCCACCA CAACCACCAC TGCCACCCCT ACTACTGAAA CCCCTCCTAC TGAAATCCCT 
ACTACTGCCA TCCCTACCAC CACCACTACT ACAACCACCA CAGCTGCCAG CACCGTCGCA 
AGCACTACAA CAACTGCAGA GAGGGCTGCG GCAGCCACCA CAAAACAGGA GCTGGCCACC 
AATGGATCGT CCATATGGGA CATAAGAGCT TGGGCTAATA GTAACTGGGC AAACATCACC 
TGGAGCCACA ATTACTCTGC AGGAACTGAC TTTGTGGTTA AGTATATCAC CAGTAACAAG 
ACAGAGAAAT CTATCCCCGT TAAAGCTCAG ACCCCCTCCT CTGTGCAGCT GGCCAATCTG 
ACGCCGGGGA TGGTGTACAA GCTGTGGGTG TTCCCCATAT GGTCTAGCCC CAGCGAGCAC 
TCGTACATAA CCTTTACCAC CAGCTCAGCT TACACCAAGA ACCACGTGGA CATCGCGACC 
CAGGGCTGGT TCATCGGGCT GATGTGTGCC ATCGCCCTCC TGGTCCTCAT TCTGCTGATT 
GTTTGCTTCA TTAAGAGGAG CAGAGGAGGG AAATACCCAG TGCGTGACAA CAAAGATGAG 
CACCTGAATC CTGAAGACAA GAACGTAGAA GATGGCTCAT TCGACTACAG GTCTCTTGAA 
AGCGATGAAG ACAACAAACC ACTGCCCAAC AGTCAGACCT CCCTGGATGG CACGATAAAG 
CAACAGGAGA GTGACGACAG CTTGGTGGAC TACGGAGAGG GTGGGGAAGG GCAGTTCAAC 
GAGGACGGCT CCTTCATTGG CCAGTACACA GTGAAAAAGG ACAAGGAGGA AACGGAAGGC 
AACGAGAGCT CAGAAGCCAC GTCCCCAGTC AATGCTATCT ACTCATTAGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154393.1
CDS	159..4232
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDLPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELATNGSSIWDIRAWANSNWANITWSHNYSAGTDFVVKYITSNKTEKSIPVKAQTPSSVQLANLTPGMVYKLWVFPIWSSPSEHSYITFTTSSAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298907.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298907.1

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG 
CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC 
AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA 
AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG 
GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC 
ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG 
AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT 
CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG 
GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG 
GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG 
AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA 
AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC 
CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC 
ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA 
GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG 
GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG 
ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG 
GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC 
CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG 
GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC 
AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG 
CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC 
CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC 
CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG 
AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC 
AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC 
GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC 
CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC 
AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT 
GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC 
GTGCTAGCAA TCCCTGCTAA CCGTTTGAGA GACTTACCTA AAGAGCGACC CGACCGGCCC 
CGGGACCTGG AGCTGTCAGA CCTGGCTGAG AGGAGCGTGA AGCTGACATG GATTCCTGGC 
GATGACAACA ACAGCCCCAT CACAGACTAC ATCGTCCAGT TTGAGGAGGA CCGCTTCCAG 
CCTGGCACGT GGCACAACCA CTCCAGGTAT CCTGGGAATG TCAACTCGGC CCTCCTGAGC 
CTCTCTCCTT ACGTCAACTA CCAATTTCGG GTGATTGCAG TGAACGACGT GGGCAGCAGC 
CTGCCCAGCA TGCCCTCAGA ACGATACCAG ACCAGCGGGG CACGACCTGA AATTAACCCA 
ACAGGAGTTC AAGGAGCAGG GACCCAAAAA AACAACATGG AGATAACCTG GACGCCTCTG 
AATGCAACTC AAGCCTATGG GCCCAACCTC CGTTACATCG TGCGGTGGAG GCGAAGGGAC 
CCACGTGGGA GTTGGTACAA CGAGACGGTG AAGGCACCAC GACACGTCGT CTGGAACACA 
CCCATCTACG TCCCCTACGA GATCAAAGTG CAGGCAGAGA ATGACTTTGG TAGAGCTCCA 
GAGCCTGAGA CCTACATCGG CTACTCAGGG GAAGATTATC CCAAGGCTGC ACCTACAGAT 
GTTAGGATAA GAGTTTTAAA CAGCACTGCC ATTGCTCTGA CATGGACCCG CGTGCACCTG 
GACACCATCC AGGGACAGCT TAAGGAGTAC AGAGCCTATT TCTGGAGAGA CAGTAGTTTG 
CTGAAGAACC TGTGGGTCTC CAAAAAACGG CAGTATGTGA GTTTTCCTGG AGACCGAAAC 
CGGGGCATAG TGTCCCGGCT GTTCCCTTAC AGCAACTACA AGCTTGAGAT GGTTGTAACC 
AACGGGAGAG GCGATGGGCC CCGCAGCGAA GTCAAGGAGT TCCCCACACC TGAAGGAGTG 
CCCAGCTCCC CCAGGTACTT AAGAATCCGA CAGCCAAATC TGGAAAGCAT CAATTTGGAG 
TGGGATCACC CAGAACATCC CAATGGAGTC CTCACGGGAT ACAACCTTAG ATATCAAGCC 
TTTAACGGAT CCAAAACGGG CAGAACCCTG GTAGAGAACT TCTCTCCCAA CCAGACAAGG 
TTCACCGTGC AGAGGACAGA CCCCATCTCG CGCTATCGAT TCTTCCTGCG TGCTCGGACA 
CAGGTGGGAG ATGGAGAAGT CATAGTGGAA GAGTCACCAG CGCTCCTGAA TGAAGCCACG 
CCAACCCCAG CCTCCACCTG GTTGCCTCCA CCTACAACCG AGCTAACTCC AGCAGCCACC 
ATTGCCACCA CAACCACCAC TGCCACCCCT ACTACTGAAA CCCCTCCTAC TGAAATCCCT 
ACTACTGCCA TCCCTACCAC CACCACTACT ACAACCACCA CAGCTGCCAG CACCGTCGCA 
AGCACTACAA CAACTGCAGA GAGGGCTGCG GCAGCCACCA CAAAACAGGA GCTGGCCACC 
AATGGATCGT CCATATGGGA CATAAGAGCT TGGGCTAATA GTAACTGGGC AAACATCACC 
TGGAGCCACA ATTACTCTGC AGGAACTGAC TTTGTGGTTA AGTATATCAC CAGTAACAAG 
ACAGAGAAAT CTATCCCCGT TAAAGCTCAG ACCCCCTCCT CTGTGCAGCT GGCCAATCTG 
ACGCCGGGGA TGGTGTACAA GCTGTGGGTG TTCCCCATAT GGTCTAGCCC CAGCGAGCAC 
TCGTACATAA CCTTTACCAC CAGCTCAGCT TACACCAAGA ACCACGTGGA CATCGCGACC 
CAGGGCTGGT TCATCGGGCT GATGTGTGCC ATCGCCCTCC TGGTCCTCAT TCTGCTGATT 
GTTTGCTTCA TTAAGAGGAG CAGAGGAGGG AAATACCCAG TGCGTGACAA CAAAGATGAG 
CACCTGAATC CTGAAGACAA GAACGTAGAA GATGGCTCAT TCGACTACAG GTCTCTTGAA 
AGCGATGAAG ACAACAAACC ACTGCCCAAC AGTCAGACCT CCCTGGATGG CACGATAAAG 
CAACAGGAGA GTGACGACAG CTTGGTGGAC TACGGAGAGG GTGGGGAAGG GCAGTTCAAC 
GAGGACGGCT CCTTCATTGG CCAGTACACA GTGAAAAAGG ACAAGGAGGA AACGGAAGGC 
AACGAGAGCT CAGAAGCCAC GTCCCCAGTC AATGCTATCT ACTCATTAGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298907.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154393.1 Gallus gallus neurofascin (NFASC), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OGa46514
Clone ID Related Accession (Same CDS sequence)	XM_015298907.1 , XM_015298907.2
Accession Version	XM_015298907.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298907.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG 
CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC 
AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA 
AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG 
GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC 
ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG 
AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT 
CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG 
GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG 
GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG 
AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA 
AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC 
CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC 
ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA 
GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG 
GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG 
ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG 
GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC 
CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG 
GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC 
AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG 
CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC 
CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC 
CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG 
AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC 
AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC 
GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC 
CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC 
AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT 
GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC 
GTGCTAGCAA TCCCTGCTAA CCGTTTGAGA GACTTACCTA AAGAGCGACC CGACCGGCCC 
CGGGACCTGG AGCTGTCAGA CCTGGCTGAG AGGAGCGTGA AGCTGACATG GATTCCTGGC 
GATGACAACA ACAGCCCCAT CACAGACTAC ATCGTCCAGT TTGAGGAGGA CCGCTTCCAG 
CCTGGCACGT GGCACAACCA CTCCAGGTAT CCTGGGAATG TCAACTCGGC CCTCCTGAGC 
CTCTCTCCTT ACGTCAACTA CCAATTTCGG GTGATTGCAG TGAACGACGT GGGCAGCAGC 
CTGCCCAGCA TGCCCTCAGA ACGATACCAG ACCAGCGGGG CACGACCTGA AATTAACCCA 
ACAGGAGTTC AAGGAGCAGG GACCCAAAAA AACAACATGG AGATAACCTG GACGCCTCTG 
AATGCAACTC AAGCCTATGG GCCCAACCTC CGTTACATCG TGCGGTGGAG GCGAAGGGAC 
CCACGTGGGA GTTGGTACAA CGAGACGGTG AAGGCACCAC GACACGTCGT CTGGAACACA 
CCCATCTACG TCCCCTACGA GATCAAAGTG CAGGCAGAGA ATGACTTTGG TAGAGCTCCA 
GAGCCTGAGA CCTACATCGG CTACTCAGGG GAAGATTATC CCAAGGCTGC ACCTACAGAT 
GTTAGGATAA GAGTTTTAAA CAGCACTGCC ATTGCTCTGA CATGGACCCG CGTGCACCTG 
GACACCATCC AGGGACAGCT TAAGGAGTAC AGAGCCTATT TCTGGAGAGA CAGTAGTTTG 
CTGAAGAACC TGTGGGTCTC CAAAAAACGG CAGTATGTGA GTTTTCCTGG AGACCGAAAC 
CGGGGCATAG TGTCCCGGCT GTTCCCTTAC AGCAACTACA AGCTTGAGAT GGTTGTAACC 
AACGGGAGAG GCGATGGGCC CCGCAGCGAA GTCAAGGAGT TCCCCACACC TGAAGGAGTG 
CCCAGCTCCC CCAGGTACTT AAGAATCCGA CAGCCAAATC TGGAAAGCAT CAATTTGGAG 
TGGGATCACC CAGAACATCC CAATGGAGTC CTCACGGGAT ACAACCTTAG ATATCAAGCC 
TTTAACGGAT CCAAAACGGG CAGAACCCTG GTAGAGAACT TCTCTCCCAA CCAGACAAGG 
TTCACCGTGC AGAGGACAGA CCCCATCTCG CGCTATCGAT TCTTCCTGCG TGCTCGGACA 
CAGGTGGGAG ATGGAGAAGT CATAGTGGAA GAGTCACCAG CGCTCCTGAA TGAAGCCACG 
CCAACCCCAG CCTCCACCTG GTTGCCTCCA CCTACAACCG AGCTAACTCC AGCAGCCACC 
ATTGCCACCA CAACCACCAC TGCCACCCCT ACTACTGAAA CCCCTCCTAC TGAAATCCCT 
ACTACTGCCA TCCCTACCAC CACCACTACT ACAACCACCA CAGCTGCCAG CACCGTCGCA 
AGCACTACAA CAACTGCAGA GAGGGCTGCG GCAGCCACCA CAAAACAGGA GCTGGCCACC 
AATGGATCGT CCATATGGGA CATAAGAGCT TGGGCTAATA GTAACTGGGC AAACATCACC 
TGGAGCCACA ATTACTCTGC AGGAACTGAC TTTGTGGTTA AGTATATCAC CAGTAACAAG 
ACAGAGAAAT CTATCCCCGT TAAAGCTCAG ACCCCCTCCT CTGTGCAGCT GGCCAATCTG 
ACGCCGGGGA TGGTGTACAA GCTGTGGGTG TTCCCCATAT GGTCTAGCCC CAGCGAGCAC 
TCGTACATAA CCTTTACCAC CAGCTCAGCT TACACCAAGA ACCACGTGGA CATCGCGACC 
CAGGGCTGGT TCATCGGGCT GATGTGTGCC ATCGCCCTCC TGGTCCTCAT TCTGCTGATT 
GTTTGCTTCA TTAAGAGGAG CAGAGGAGGG AAATACCCAG TGCGTGACAA CAAAGATGAG 
CACCTGAATC CTGAAGACAA GAACGTAGAA GATGGCTCAT TCGACTACAG GTCTCTTGAA 
AGCGATGAAG ACAACAAACC ACTGCCCAAC AGTCAGACCT CCCTGGATGG CACGATAAAG 
CAACAGGAGA GTGACGACAG CTTGGTGGAC TACGGAGAGG GTGGGGAAGG GCAGTTCAAC 
GAGGACGGCT CCTTCATTGG CCAGTACACA GTGAAAAAGG ACAAGGAGGA AACGGAAGGC 
AACGAGAGCT CAGAAGCCAC GTCCCCAGTC AATGCTATCT ACTCATTAGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154393.1
CDS	169..4242
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDLPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELATNGSSIWDIRAWANSNWANITWSHNYSAGTDFVVKYITSNKTEKSIPVKAQTPSSVQLANLTPGMVYKLWVFPIWSSPSEHSYITFTTSSAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298907.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298907.2

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG 
CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC 
AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA 
AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG 
GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC 
ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG 
AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT 
CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG 
GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG 
GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG 
AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA 
AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC 
CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC 
ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA 
GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG 
GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG 
ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG 
GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC 
CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG 
GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC 
AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG 
CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC 
CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC 
CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG 
AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC 
AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC 
GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC 
CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC 
AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT 
GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC 
GTGCTAGCAA TCCCTGCTAA CCGTTTGAGA GACTTACCTA AAGAGCGACC CGACCGGCCC 
CGGGACCTGG AGCTGTCAGA CCTGGCTGAG AGGAGCGTGA AGCTGACATG GATTCCTGGC 
GATGACAACA ACAGCCCCAT CACAGACTAC ATCGTCCAGT TTGAGGAGGA CCGCTTCCAG 
CCTGGCACGT GGCACAACCA CTCCAGGTAT CCTGGGAATG TCAACTCGGC CCTCCTGAGC 
CTCTCTCCTT ACGTCAACTA CCAATTTCGG GTGATTGCAG TGAACGACGT GGGCAGCAGC 
CTGCCCAGCA TGCCCTCAGA ACGATACCAG ACCAGCGGGG CACGACCTGA AATTAACCCA 
ACAGGAGTTC AAGGAGCAGG GACCCAAAAA AACAACATGG AGATAACCTG GACGCCTCTG 
AATGCAACTC AAGCCTATGG GCCCAACCTC CGTTACATCG TGCGGTGGAG GCGAAGGGAC 
CCACGTGGGA GTTGGTACAA CGAGACGGTG AAGGCACCAC GACACGTCGT CTGGAACACA 
CCCATCTACG TCCCCTACGA GATCAAAGTG CAGGCAGAGA ATGACTTTGG TAGAGCTCCA 
GAGCCTGAGA CCTACATCGG CTACTCAGGG GAAGATTATC CCAAGGCTGC ACCTACAGAT 
GTTAGGATAA GAGTTTTAAA CAGCACTGCC ATTGCTCTGA CATGGACCCG CGTGCACCTG 
GACACCATCC AGGGACAGCT TAAGGAGTAC AGAGCCTATT TCTGGAGAGA CAGTAGTTTG 
CTGAAGAACC TGTGGGTCTC CAAAAAACGG CAGTATGTGA GTTTTCCTGG AGACCGAAAC 
CGGGGCATAG TGTCCCGGCT GTTCCCTTAC AGCAACTACA AGCTTGAGAT GGTTGTAACC 
AACGGGAGAG GCGATGGGCC CCGCAGCGAA GTCAAGGAGT TCCCCACACC TGAAGGAGTG 
CCCAGCTCCC CCAGGTACTT AAGAATCCGA CAGCCAAATC TGGAAAGCAT CAATTTGGAG 
TGGGATCACC CAGAACATCC CAATGGAGTC CTCACGGGAT ACAACCTTAG ATATCAAGCC 
TTTAACGGAT CCAAAACGGG CAGAACCCTG GTAGAGAACT TCTCTCCCAA CCAGACAAGG 
TTCACCGTGC AGAGGACAGA CCCCATCTCG CGCTATCGAT TCTTCCTGCG TGCTCGGACA 
CAGGTGGGAG ATGGAGAAGT CATAGTGGAA GAGTCACCAG CGCTCCTGAA TGAAGCCACG 
CCAACCCCAG CCTCCACCTG GTTGCCTCCA CCTACAACCG AGCTAACTCC AGCAGCCACC 
ATTGCCACCA CAACCACCAC TGCCACCCCT ACTACTGAAA CCCCTCCTAC TGAAATCCCT 
ACTACTGCCA TCCCTACCAC CACCACTACT ACAACCACCA CAGCTGCCAG CACCGTCGCA 
AGCACTACAA CAACTGCAGA GAGGGCTGCG GCAGCCACCA CAAAACAGGA GCTGGCCACC 
AATGGATCGT CCATATGGGA CATAAGAGCT TGGGCTAATA GTAACTGGGC AAACATCACC 
TGGAGCCACA ATTACTCTGC AGGAACTGAC TTTGTGGTTA AGTATATCAC CAGTAACAAG 
ACAGAGAAAT CTATCCCCGT TAAAGCTCAG ACCCCCTCCT CTGTGCAGCT GGCCAATCTG 
ACGCCGGGGA TGGTGTACAA GCTGTGGGTG TTCCCCATAT GGTCTAGCCC CAGCGAGCAC 
TCGTACATAA CCTTTACCAC CAGCTCAGCT TACACCAAGA ACCACGTGGA CATCGCGACC 
CAGGGCTGGT TCATCGGGCT GATGTGTGCC ATCGCCCTCC TGGTCCTCAT TCTGCTGATT 
GTTTGCTTCA TTAAGAGGAG CAGAGGAGGG AAATACCCAG TGCGTGACAA CAAAGATGAG 
CACCTGAATC CTGAAGACAA GAACGTAGAA GATGGCTCAT TCGACTACAG GTCTCTTGAA 
AGCGATGAAG ACAACAAACC ACTGCCCAAC AGTCAGACCT CCCTGGATGG CACGATAAAG 
CAACAGGAGA GTGACGACAG CTTGGTGGAC TACGGAGAGG GTGGGGAAGG GCAGTTCAAC 
GAGGACGGCT CCTTCATTGG CCAGTACACA GTGAAAAAGG ACAAGGAGGA AACGGAAGGC 
AACGAGAGCT CAGAAGCCAC GTCCCCAGTC AATGCTATCT ACTCATTAGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298908.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154394.1 Gallus gallus neurofascin (NFASC), transcript variant X6, mRNA.

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CloneID	OGa46515
Clone ID Related Accession (Same CDS sequence)	XM_015298908.1 , XM_015298908.2
Accession Version	XM_015298908.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGAGCGA CCCGACCGGC CCCGGGACCT GGAGCTGTCA 
GACCTGGCTG AGAGGAGCGT GAAGCTGACA TGGATTCCTG GCGATGACAA CAACAGCCCC 
ATCACAGACT ACATCGTCCA GTTTGAGGAG GACCGCTTCC AGCCTGGCAC GTGGCACAAC 
CACTCCAGGT ATCCTGGGAA TGTCAACTCG GCCCTCCTGA GCCTCTCTCC TTACGTCAAC 
TACCAATTTC GGGTGATTGC AGTGAACGAC GTGGGCAGCA GCCTGCCCAG CATGCCCTCA 
GAACGATACC AGACCAGCGG GGCACGACCT GAAATTAACC CAACAGGAGT TCAAGGAGCA 
GGGACCCAAA AAAACAACAT GGAGATAACC TGGACGCCTC TGAATGCAAC TCAAGCCTAT 
GGGCCCAACC TCCGTTACAT CGTGCGGTGG AGGCGAAGGG ACCCACGTGG GAGTTGGTAC 
AACGAGACGG TGAAGGCACC ACGACACGTC GTCTGGAACA CACCCATCTA CGTCCCCTAC 
GAGATCAAAG TGCAGGCAGA GAATGACTTT GGTAGAGCTC CAGAGCCTGA GACCTACATC 
GGCTACTCAG GGGAAGATTA TCCCAAGGCT GCACCTACAG ATGTTAGGAT AAGAGTTTTA 
AACAGCACTG CCATTGCTCT GACATGGACC CGCGTGCACC TGGACACCAT CCAGGGACAG 
CTTAAGGAGT ACAGAGCCTA TTTCTGGAGA GACAGTAGTT TGCTGAAGAA CCTGTGGGTC 
TCCAAAAAAC GGCAGTATGT GAGTTTTCCT GGAGACCGAA ACCGGGGCAT AGTGTCCCGG 
CTGTTCCCTT ACAGCAACTA CAAGCTTGAG ATGGTTGTAA CCAACGGGAG AGGCGATGGG 
CCCCGCAGCG AAGTCAAGGA GTTCCCCACA CCTGAAGGAG TGCCCAGCTC CCCCAGGTAC 
TTAAGAATCC GACAGCCAAA TCTGGAAAGC ATCAATTTGG AGTGGGATCA CCCAGAACAT 
CCCAATGGAG TCCTCACGGG ATACAACCTT AGATATCAAG CCTTTAACGG ATCCAAAACG 
GGCAGAACCC TGGTAGAGAA CTTCTCTCCC AACCAGACAA GGTTCACCGT GCAGAGGACA 
GACCCCATCT CGCGCTATCG ATTCTTCCTG CGTGCTCGGA CACAGGTGGG AGATGGAGAA 
GTCATAGTGG AAGAGTCACC AGCGCTCCTG AATGAAGCCA CGCCAACCCC AGCCTCCACC 
TGGTTGCCTC CACCTACAAC CGAGCTAACT CCAGCAGCCA CCATTGCCAC CACAACCACC 
ACTGCCACCC CTACTACTGA AACCCCTCCT ACTGAAATCC CTACTACTGC CATCCCTACC 
ACCACCACTA CTACAACCAC CACAGCTGCC AGCACCGTCG CAAGCACTAC AACAACTGCA 
GAGAGGGCTG CGGCAGCCAC CACAAAACAG GAGCTGGCCA CCAATGGATC GTCCATATGG 
GACATAAGAG CTTGGGCTAA TAGTAACTGG GCAAACATCA CCTGGAGCCA CAATTACTCT 
GCAGGAACTG ACTTTGTGGT TAAGTATATC ACCAGTAACA AGACAGAGAA ATCTATCCCC 
GTTAAAGCTC AGACCCCCTC CTCTGTGCAG CTGGCCAATC TGACGCCGGG GATGGTGTAC 
AAGCTGTGGG TGTTCCCCAT ATGGTCTAGC CCCAGCGAGC ACTCGTACAT AACCTTTACC 
ACCAGCTCAG CTTACACCAA GAACCACGTG GACATCGCGA CCCAGGGCTG GTTCATCGGG 
CTGATGTGTG CCATCGCCCT CCTGGTCCTC ATTCTGCTGA TTGTTTGCTT CATTAAGAGG 
AGCAGAGGAG GGAAATACCC AGTGCGTGAC AACAAAGATG AGCACCTGAA TCCTGAAGAC 
AAGAACGTAG AAGATGGCTC ATTCGACTAC AGGTCTCTTG AAAGCGATGA AGACAACAAA 
CCACTGCCCA ACAGTCAGAC CTCCCTGGAT GGCACGATAA AGCAACAGGA GAGTGACGAC 
AGCTTGGTGG ACTACGGAGA GGGTGGGGAA GGGCAGTTCA ACGAGGACGG CTCCTTCATT 
GGCCAGTACA CAGTGAAAAA GGACAAGGAG GAAACGGAAG GCAACGAGAG CTCAGAAGCC 
ACGTCCCCAG TCAATGCTAT CTACTCATTA GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154394.1
CDS	159..4214
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELATNGSSIWDIRAWANSNWANITWSHNYSAGTDFVVKYITSNKTEKSIPVKAQTPSSVQLANLTPGMVYKLWVFPIWSSPSEHSYITFTTSSAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298908.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298908.1

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGAGCGA CCCGACCGGC CCCGGGACCT GGAGCTGTCA 
GACCTGGCTG AGAGGAGCGT GAAGCTGACA TGGATTCCTG GCGATGACAA CAACAGCCCC 
ATCACAGACT ACATCGTCCA GTTTGAGGAG GACCGCTTCC AGCCTGGCAC GTGGCACAAC 
CACTCCAGGT ATCCTGGGAA TGTCAACTCG GCCCTCCTGA GCCTCTCTCC TTACGTCAAC 
TACCAATTTC GGGTGATTGC AGTGAACGAC GTGGGCAGCA GCCTGCCCAG CATGCCCTCA 
GAACGATACC AGACCAGCGG GGCACGACCT GAAATTAACC CAACAGGAGT TCAAGGAGCA 
GGGACCCAAA AAAACAACAT GGAGATAACC TGGACGCCTC TGAATGCAAC TCAAGCCTAT 
GGGCCCAACC TCCGTTACAT CGTGCGGTGG AGGCGAAGGG ACCCACGTGG GAGTTGGTAC 
AACGAGACGG TGAAGGCACC ACGACACGTC GTCTGGAACA CACCCATCTA CGTCCCCTAC 
GAGATCAAAG TGCAGGCAGA GAATGACTTT GGTAGAGCTC CAGAGCCTGA GACCTACATC 
GGCTACTCAG GGGAAGATTA TCCCAAGGCT GCACCTACAG ATGTTAGGAT AAGAGTTTTA 
AACAGCACTG CCATTGCTCT GACATGGACC CGCGTGCACC TGGACACCAT CCAGGGACAG 
CTTAAGGAGT ACAGAGCCTA TTTCTGGAGA GACAGTAGTT TGCTGAAGAA CCTGTGGGTC 
TCCAAAAAAC GGCAGTATGT GAGTTTTCCT GGAGACCGAA ACCGGGGCAT AGTGTCCCGG 
CTGTTCCCTT ACAGCAACTA CAAGCTTGAG ATGGTTGTAA CCAACGGGAG AGGCGATGGG 
CCCCGCAGCG AAGTCAAGGA GTTCCCCACA CCTGAAGGAG TGCCCAGCTC CCCCAGGTAC 
TTAAGAATCC GACAGCCAAA TCTGGAAAGC ATCAATTTGG AGTGGGATCA CCCAGAACAT 
CCCAATGGAG TCCTCACGGG ATACAACCTT AGATATCAAG CCTTTAACGG ATCCAAAACG 
GGCAGAACCC TGGTAGAGAA CTTCTCTCCC AACCAGACAA GGTTCACCGT GCAGAGGACA 
GACCCCATCT CGCGCTATCG ATTCTTCCTG CGTGCTCGGA CACAGGTGGG AGATGGAGAA 
GTCATAGTGG AAGAGTCACC AGCGCTCCTG AATGAAGCCA CGCCAACCCC AGCCTCCACC 
TGGTTGCCTC CACCTACAAC CGAGCTAACT CCAGCAGCCA CCATTGCCAC CACAACCACC 
ACTGCCACCC CTACTACTGA AACCCCTCCT ACTGAAATCC CTACTACTGC CATCCCTACC 
ACCACCACTA CTACAACCAC CACAGCTGCC AGCACCGTCG CAAGCACTAC AACAACTGCA 
GAGAGGGCTG CGGCAGCCAC CACAAAACAG GAGCTGGCCA CCAATGGATC GTCCATATGG 
GACATAAGAG CTTGGGCTAA TAGTAACTGG GCAAACATCA CCTGGAGCCA CAATTACTCT 
GCAGGAACTG ACTTTGTGGT TAAGTATATC ACCAGTAACA AGACAGAGAA ATCTATCCCC 
GTTAAAGCTC AGACCCCCTC CTCTGTGCAG CTGGCCAATC TGACGCCGGG GATGGTGTAC 
AAGCTGTGGG TGTTCCCCAT ATGGTCTAGC CCCAGCGAGC ACTCGTACAT AACCTTTACC 
ACCAGCTCAG CTTACACCAA GAACCACGTG GACATCGCGA CCCAGGGCTG GTTCATCGGG 
CTGATGTGTG CCATCGCCCT CCTGGTCCTC ATTCTGCTGA TTGTTTGCTT CATTAAGAGG 
AGCAGAGGAG GGAAATACCC AGTGCGTGAC AACAAAGATG AGCACCTGAA TCCTGAAGAC 
AAGAACGTAG AAGATGGCTC ATTCGACTAC AGGTCTCTTG AAAGCGATGA AGACAACAAA 
CCACTGCCCA ACAGTCAGAC CTCCCTGGAT GGCACGATAA AGCAACAGGA GAGTGACGAC 
AGCTTGGTGG ACTACGGAGA GGGTGGGGAA GGGCAGTTCA ACGAGGACGG CTCCTTCATT 
GGCCAGTACA CAGTGAAAAA GGACAAGGAG GAAACGGAAG GCAACGAGAG CTCAGAAGCC 
ACGTCCCCAG TCAATGCTAT CTACTCATTA GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298908.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154394.1 Gallus gallus neurofascin (NFASC), transcript variant X6, mRNA.

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CloneID	OGa46515
Clone ID Related Accession (Same CDS sequence)	XM_015298908.1 , XM_015298908.2
Accession Version	XM_015298908.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298908.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGAGCGA CCCGACCGGC CCCGGGACCT GGAGCTGTCA 
GACCTGGCTG AGAGGAGCGT GAAGCTGACA TGGATTCCTG GCGATGACAA CAACAGCCCC 
ATCACAGACT ACATCGTCCA GTTTGAGGAG GACCGCTTCC AGCCTGGCAC GTGGCACAAC 
CACTCCAGGT ATCCTGGGAA TGTCAACTCG GCCCTCCTGA GCCTCTCTCC TTACGTCAAC 
TACCAATTTC GGGTGATTGC AGTGAACGAC GTGGGCAGCA GCCTGCCCAG CATGCCCTCA 
GAACGATACC AGACCAGCGG GGCACGACCT GAAATTAACC CAACAGGAGT TCAAGGAGCA 
GGGACCCAAA AAAACAACAT GGAGATAACC TGGACGCCTC TGAATGCAAC TCAAGCCTAT 
GGGCCCAACC TCCGTTACAT CGTGCGGTGG AGGCGAAGGG ACCCACGTGG GAGTTGGTAC 
AACGAGACGG TGAAGGCACC ACGACACGTC GTCTGGAACA CACCCATCTA CGTCCCCTAC 
GAGATCAAAG TGCAGGCAGA GAATGACTTT GGTAGAGCTC CAGAGCCTGA GACCTACATC 
GGCTACTCAG GGGAAGATTA TCCCAAGGCT GCACCTACAG ATGTTAGGAT AAGAGTTTTA 
AACAGCACTG CCATTGCTCT GACATGGACC CGCGTGCACC TGGACACCAT CCAGGGACAG 
CTTAAGGAGT ACAGAGCCTA TTTCTGGAGA GACAGTAGTT TGCTGAAGAA CCTGTGGGTC 
TCCAAAAAAC GGCAGTATGT GAGTTTTCCT GGAGACCGAA ACCGGGGCAT AGTGTCCCGG 
CTGTTCCCTT ACAGCAACTA CAAGCTTGAG ATGGTTGTAA CCAACGGGAG AGGCGATGGG 
CCCCGCAGCG AAGTCAAGGA GTTCCCCACA CCTGAAGGAG TGCCCAGCTC CCCCAGGTAC 
TTAAGAATCC GACAGCCAAA TCTGGAAAGC ATCAATTTGG AGTGGGATCA CCCAGAACAT 
CCCAATGGAG TCCTCACGGG ATACAACCTT AGATATCAAG CCTTTAACGG ATCCAAAACG 
GGCAGAACCC TGGTAGAGAA CTTCTCTCCC AACCAGACAA GGTTCACCGT GCAGAGGACA 
GACCCCATCT CGCGCTATCG ATTCTTCCTG CGTGCTCGGA CACAGGTGGG AGATGGAGAA 
GTCATAGTGG AAGAGTCACC AGCGCTCCTG AATGAAGCCA CGCCAACCCC AGCCTCCACC 
TGGTTGCCTC CACCTACAAC CGAGCTAACT CCAGCAGCCA CCATTGCCAC CACAACCACC 
ACTGCCACCC CTACTACTGA AACCCCTCCT ACTGAAATCC CTACTACTGC CATCCCTACC 
ACCACCACTA CTACAACCAC CACAGCTGCC AGCACCGTCG CAAGCACTAC AACAACTGCA 
GAGAGGGCTG CGGCAGCCAC CACAAAACAG GAGCTGGCCA CCAATGGATC GTCCATATGG 
GACATAAGAG CTTGGGCTAA TAGTAACTGG GCAAACATCA CCTGGAGCCA CAATTACTCT 
GCAGGAACTG ACTTTGTGGT TAAGTATATC ACCAGTAACA AGACAGAGAA ATCTATCCCC 
GTTAAAGCTC AGACCCCCTC CTCTGTGCAG CTGGCCAATC TGACGCCGGG GATGGTGTAC 
AAGCTGTGGG TGTTCCCCAT ATGGTCTAGC CCCAGCGAGC ACTCGTACAT AACCTTTACC 
ACCAGCTCAG CTTACACCAA GAACCACGTG GACATCGCGA CCCAGGGCTG GTTCATCGGG 
CTGATGTGTG CCATCGCCCT CCTGGTCCTC ATTCTGCTGA TTGTTTGCTT CATTAAGAGG 
AGCAGAGGAG GGAAATACCC AGTGCGTGAC AACAAAGATG AGCACCTGAA TCCTGAAGAC 
AAGAACGTAG AAGATGGCTC ATTCGACTAC AGGTCTCTTG AAAGCGATGA AGACAACAAA 
CCACTGCCCA ACAGTCAGAC CTCCCTGGAT GGCACGATAA AGCAACAGGA GAGTGACGAC 
AGCTTGGTGG ACTACGGAGA GGGTGGGGAA GGGCAGTTCA ACGAGGACGG CTCCTTCATT 
GGCCAGTACA CAGTGAAAAA GGACAAGGAG GAAACGGAAG GCAACGAGAG CTCAGAAGCC 
ACGTCCCCAG TCAATGCTAT CTACTCATTA GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154394.1
CDS	169..4224
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELATNGSSIWDIRAWANSNWANITWSHNYSAGTDFVVKYITSNKTEKSIPVKAQTPSSVQLANLTPGMVYKLWVFPIWSSPSEHSYITFTTSSAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298908.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298908.2

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGAGCGA CCCGACCGGC CCCGGGACCT GGAGCTGTCA 
GACCTGGCTG AGAGGAGCGT GAAGCTGACA TGGATTCCTG GCGATGACAA CAACAGCCCC 
ATCACAGACT ACATCGTCCA GTTTGAGGAG GACCGCTTCC AGCCTGGCAC GTGGCACAAC 
CACTCCAGGT ATCCTGGGAA TGTCAACTCG GCCCTCCTGA GCCTCTCTCC TTACGTCAAC 
TACCAATTTC GGGTGATTGC AGTGAACGAC GTGGGCAGCA GCCTGCCCAG CATGCCCTCA 
GAACGATACC AGACCAGCGG GGCACGACCT GAAATTAACC CAACAGGAGT TCAAGGAGCA 
GGGACCCAAA AAAACAACAT GGAGATAACC TGGACGCCTC TGAATGCAAC TCAAGCCTAT 
GGGCCCAACC TCCGTTACAT CGTGCGGTGG AGGCGAAGGG ACCCACGTGG GAGTTGGTAC 
AACGAGACGG TGAAGGCACC ACGACACGTC GTCTGGAACA CACCCATCTA CGTCCCCTAC 
GAGATCAAAG TGCAGGCAGA GAATGACTTT GGTAGAGCTC CAGAGCCTGA GACCTACATC 
GGCTACTCAG GGGAAGATTA TCCCAAGGCT GCACCTACAG ATGTTAGGAT AAGAGTTTTA 
AACAGCACTG CCATTGCTCT GACATGGACC CGCGTGCACC TGGACACCAT CCAGGGACAG 
CTTAAGGAGT ACAGAGCCTA TTTCTGGAGA GACAGTAGTT TGCTGAAGAA CCTGTGGGTC 
TCCAAAAAAC GGCAGTATGT GAGTTTTCCT GGAGACCGAA ACCGGGGCAT AGTGTCCCGG 
CTGTTCCCTT ACAGCAACTA CAAGCTTGAG ATGGTTGTAA CCAACGGGAG AGGCGATGGG 
CCCCGCAGCG AAGTCAAGGA GTTCCCCACA CCTGAAGGAG TGCCCAGCTC CCCCAGGTAC 
TTAAGAATCC GACAGCCAAA TCTGGAAAGC ATCAATTTGG AGTGGGATCA CCCAGAACAT 
CCCAATGGAG TCCTCACGGG ATACAACCTT AGATATCAAG CCTTTAACGG ATCCAAAACG 
GGCAGAACCC TGGTAGAGAA CTTCTCTCCC AACCAGACAA GGTTCACCGT GCAGAGGACA 
GACCCCATCT CGCGCTATCG ATTCTTCCTG CGTGCTCGGA CACAGGTGGG AGATGGAGAA 
GTCATAGTGG AAGAGTCACC AGCGCTCCTG AATGAAGCCA CGCCAACCCC AGCCTCCACC 
TGGTTGCCTC CACCTACAAC CGAGCTAACT CCAGCAGCCA CCATTGCCAC CACAACCACC 
ACTGCCACCC CTACTACTGA AACCCCTCCT ACTGAAATCC CTACTACTGC CATCCCTACC 
ACCACCACTA CTACAACCAC CACAGCTGCC AGCACCGTCG CAAGCACTAC AACAACTGCA 
GAGAGGGCTG CGGCAGCCAC CACAAAACAG GAGCTGGCCA CCAATGGATC GTCCATATGG 
GACATAAGAG CTTGGGCTAA TAGTAACTGG GCAAACATCA CCTGGAGCCA CAATTACTCT 
GCAGGAACTG ACTTTGTGGT TAAGTATATC ACCAGTAACA AGACAGAGAA ATCTATCCCC 
GTTAAAGCTC AGACCCCCTC CTCTGTGCAG CTGGCCAATC TGACGCCGGG GATGGTGTAC 
AAGCTGTGGG TGTTCCCCAT ATGGTCTAGC CCCAGCGAGC ACTCGTACAT AACCTTTACC 
ACCAGCTCAG CTTACACCAA GAACCACGTG GACATCGCGA CCCAGGGCTG GTTCATCGGG 
CTGATGTGTG CCATCGCCCT CCTGGTCCTC ATTCTGCTGA TTGTTTGCTT CATTAAGAGG 
AGCAGAGGAG GGAAATACCC AGTGCGTGAC AACAAAGATG AGCACCTGAA TCCTGAAGAC 
AAGAACGTAG AAGATGGCTC ATTCGACTAC AGGTCTCTTG AAAGCGATGA AGACAACAAA 
CCACTGCCCA ACAGTCAGAC CTCCCTGGAT GGCACGATAA AGCAACAGGA GAGTGACGAC 
AGCTTGGTGG ACTACGGAGA GGGTGGGGAA GGGCAGTTCA ACGAGGACGG CTCCTTCATT 
GGCCAGTACA CAGTGAAAAA GGACAAGGAG GAAACGGAAG GCAACGAGAG CTCAGAAGCC 
ACGTCCCCAG TCAATGCTAT CTACTCATTA GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298909.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154395.1 Gallus gallus neurofascin (NFASC), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OGa46516
Clone ID Related Accession (Same CDS sequence)	XM_015298909.1 , XM_015298909.2
Accession Version	XM_015298909.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA 
ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC 
CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG 
TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG 
GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC 
AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG 
CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA 
GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT 
TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG 
ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC 
AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC 
ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC 
CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG 
GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC 
ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA 
GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT 
CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG 
GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA 
TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC 
AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC 
TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG 
AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC 
GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT 
GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG 
ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC 
AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC 
AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT 
TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG 
GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG 
GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA 
GATTATCCCA AGGCTGCACC TACAGATGTT AGGATAAGAG TTTTAAACAG CACTGCCATT 
GCTCTGACAT GGACCCGCGT GCACCTGGAC ACCATCCAGG GACAGCTTAA GGAGTACAGA 
GCCTATTTCT GGAGAGACAG TAGTTTGCTG AAGAACCTGT GGGTCTCCAA AAAACGGCAG 
TATGTGAGTT TTCCTGGAGA CCGAAACCGG GGCATAGTGT CCCGGCTGTT CCCTTACAGC 
AACTACAAGC TTGAGATGGT TGTAACCAAC GGGAGAGGCG ATGGGCCCCG CAGCGAAGTC 
AAGGAGTTCC CCACACCTGA AGGAGTGCCC AGCTCCCCCA GGTACTTAAG AATCCGACAG 
CCAAATCTGG AAAGCATCAA TTTGGAGTGG GATCACCCAG AACATCCCAA TGGAGTCCTC 
ACGGGATACA ACCTTAGATA TCAAGCCTTT AACGGATCCA AAACGGGCAG AACCCTGGTA 
GAGAACTTCT CTCCCAACCA GACAAGGTTC ACCGTGCAGA GGACAGACCC CATCTCGCGC 
TATCGATTCT TCCTGCGTGC TCGGACACAG GTGGGAGATG GAGAAGTCAT AGTGGAAGAG 
TCACCAGCGC TCCTGAATGA AGCCACGCCA ACCCCAGCCT CCACCTGGTT GCCTCCACCT 
ACAACCGAGC TAACTCCAGC AGCCACCATT GCCACCACAA CCACCACTGC CACCCCTACT 
ACTGAAACCC CTCCTACTGA AATCCCTACT ACTGCCATCC CTACCACCAC CACTACTACA 
ACCACCACAG CTGCCAGCAC CGTCGCAAGC ACTACAACAA CTGCAGAGAG GGCTGCGGCA 
GCCACCACAA AACAGGAGCT GGCCACCAAT GGATCGTCCA TATGGGACAT AAGAGCTTGG 
GCTAATAGTA ACTGGGCAAA CATCACCTGG AGCCACAATT ACTCTGCAGG AACTGACTTT 
GTGGTTAAGT ATATCACCAG TAACAAGACA GAGAAATCTA TCCCCGTTAA AGCTCAGACC 
CCCTCCTCTG TGCAGCTGGC CAATCTGACG CCGGGGATGG TGTACAAGCT GTGGGTGTTC 
CCCATATGGT CTAGCCCCAG CGAGCACTCG TACATAACCT TTACCACCAG CTCAGCTTAC 
ACCAAGAACC ACGTGGACAT CGCGACCCAG GGCTGGTTCA TCGGGCTGAT GTGTGCCATC 
GCCCTCCTGG TCCTCATTCT GCTGATTGTT TGCTTCATTA AGAGGAGCAG AGGAGGGAAA 
TACCCAGTGC GTGACAACAA AGATGAGCAC CTGAATCCTG AAGACAAGAA CGTAGAAGAT 
GGCTCATTCG ACTACAGGTC TCTTGAAAGC GATGAAGACA ACAAACCACT GCCCAACAGT 
CAGACCTCCC TGGATGGCAC GATAAAGCAA CAGGAGAGTG ACGACAGCTT GGTGGACTAC 
GGAGAGGGTG GGGAAGGGCA GTTCAACGAG GACGGCTCCT TCATTGGCCA GTACACAGTG 
AAAAAGGACA AGGAGGAAAC GGAAGGCAAC GAGAGCTCAG AAGCCACGTC CCCAGTCAAT 
GCTATCTACT CATTAGCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154395.1
CDS	159..4199
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTTRGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELATNGSSIWDIRAWANSNWANITWSHNYSAGTDFVVKYITSNKTEKSIPVKAQTPSSVQLANLTPGMVYKLWVFPIWSSPSEHSYITFTTSSAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298909.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298909.1

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA 
ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC 
CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG 
TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG 
GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC 
AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG 
CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA 
GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT 
TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG 
ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC 
AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC 
ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC 
CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG 
GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC 
ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA 
GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT 
CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG 
GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA 
TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC 
AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC 
TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG 
AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC 
GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT 
GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG 
ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC 
AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC 
AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT 
TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG 
GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG 
GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA 
GATTATCCCA AGGCTGCACC TACAGATGTT AGGATAAGAG TTTTAAACAG CACTGCCATT 
GCTCTGACAT GGACCCGCGT GCACCTGGAC ACCATCCAGG GACAGCTTAA GGAGTACAGA 
GCCTATTTCT GGAGAGACAG TAGTTTGCTG AAGAACCTGT GGGTCTCCAA AAAACGGCAG 
TATGTGAGTT TTCCTGGAGA CCGAAACCGG GGCATAGTGT CCCGGCTGTT CCCTTACAGC 
AACTACAAGC TTGAGATGGT TGTAACCAAC GGGAGAGGCG ATGGGCCCCG CAGCGAAGTC 
AAGGAGTTCC CCACACCTGA AGGAGTGCCC AGCTCCCCCA GGTACTTAAG AATCCGACAG 
CCAAATCTGG AAAGCATCAA TTTGGAGTGG GATCACCCAG AACATCCCAA TGGAGTCCTC 
ACGGGATACA ACCTTAGATA TCAAGCCTTT AACGGATCCA AAACGGGCAG AACCCTGGTA 
GAGAACTTCT CTCCCAACCA GACAAGGTTC ACCGTGCAGA GGACAGACCC CATCTCGCGC 
TATCGATTCT TCCTGCGTGC TCGGACACAG GTGGGAGATG GAGAAGTCAT AGTGGAAGAG 
TCACCAGCGC TCCTGAATGA AGCCACGCCA ACCCCAGCCT CCACCTGGTT GCCTCCACCT 
ACAACCGAGC TAACTCCAGC AGCCACCATT GCCACCACAA CCACCACTGC CACCCCTACT 
ACTGAAACCC CTCCTACTGA AATCCCTACT ACTGCCATCC CTACCACCAC CACTACTACA 
ACCACCACAG CTGCCAGCAC CGTCGCAAGC ACTACAACAA CTGCAGAGAG GGCTGCGGCA 
GCCACCACAA AACAGGAGCT GGCCACCAAT GGATCGTCCA TATGGGACAT AAGAGCTTGG 
GCTAATAGTA ACTGGGCAAA CATCACCTGG AGCCACAATT ACTCTGCAGG AACTGACTTT 
GTGGTTAAGT ATATCACCAG TAACAAGACA GAGAAATCTA TCCCCGTTAA AGCTCAGACC 
CCCTCCTCTG TGCAGCTGGC CAATCTGACG CCGGGGATGG TGTACAAGCT GTGGGTGTTC 
CCCATATGGT CTAGCCCCAG CGAGCACTCG TACATAACCT TTACCACCAG CTCAGCTTAC 
ACCAAGAACC ACGTGGACAT CGCGACCCAG GGCTGGTTCA TCGGGCTGAT GTGTGCCATC 
GCCCTCCTGG TCCTCATTCT GCTGATTGTT TGCTTCATTA AGAGGAGCAG AGGAGGGAAA 
TACCCAGTGC GTGACAACAA AGATGAGCAC CTGAATCCTG AAGACAAGAA CGTAGAAGAT 
GGCTCATTCG ACTACAGGTC TCTTGAAAGC GATGAAGACA ACAAACCACT GCCCAACAGT 
CAGACCTCCC TGGATGGCAC GATAAAGCAA CAGGAGAGTG ACGACAGCTT GGTGGACTAC 
GGAGAGGGTG GGGAAGGGCA GTTCAACGAG GACGGCTCCT TCATTGGCCA GTACACAGTG 
AAAAAGGACA AGGAGGAAAC GGAAGGCAAC GAGAGCTCAG AAGCCACGTC CCCAGTCAAT 
GCTATCTACT CATTAGCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298909.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154395.1 Gallus gallus neurofascin (NFASC), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OGa46516
Clone ID Related Accession (Same CDS sequence)	XM_015298909.1 , XM_015298909.2
Accession Version	XM_015298909.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298909.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA 
ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC 
CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG 
TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG 
GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC 
AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG 
CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA 
GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT 
TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG 
ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC 
AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC 
ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC 
CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG 
GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC 
ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA 
GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT 
CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG 
GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA 
TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC 
AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC 
TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG 
AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC 
GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT 
GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG 
ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC 
AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC 
AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT 
TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG 
GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG 
GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA 
GATTATCCCA AGGCTGCACC TACAGATGTT AGGATAAGAG TTTTAAACAG CACTGCCATT 
GCTCTGACAT GGACCCGCGT GCACCTGGAC ACCATCCAGG GACAGCTTAA GGAGTACAGA 
GCCTATTTCT GGAGAGACAG TAGTTTGCTG AAGAACCTGT GGGTCTCCAA AAAACGGCAG 
TATGTGAGTT TTCCTGGAGA CCGAAACCGG GGCATAGTGT CCCGGCTGTT CCCTTACAGC 
AACTACAAGC TTGAGATGGT TGTAACCAAC GGGAGAGGCG ATGGGCCCCG CAGCGAAGTC 
AAGGAGTTCC CCACACCTGA AGGAGTGCCC AGCTCCCCCA GGTACTTAAG AATCCGACAG 
CCAAATCTGG AAAGCATCAA TTTGGAGTGG GATCACCCAG AACATCCCAA TGGAGTCCTC 
ACGGGATACA ACCTTAGATA TCAAGCCTTT AACGGATCCA AAACGGGCAG AACCCTGGTA 
GAGAACTTCT CTCCCAACCA GACAAGGTTC ACCGTGCAGA GGACAGACCC CATCTCGCGC 
TATCGATTCT TCCTGCGTGC TCGGACACAG GTGGGAGATG GAGAAGTCAT AGTGGAAGAG 
TCACCAGCGC TCCTGAATGA AGCCACGCCA ACCCCAGCCT CCACCTGGTT GCCTCCACCT 
ACAACCGAGC TAACTCCAGC AGCCACCATT GCCACCACAA CCACCACTGC CACCCCTACT 
ACTGAAACCC CTCCTACTGA AATCCCTACT ACTGCCATCC CTACCACCAC CACTACTACA 
ACCACCACAG CTGCCAGCAC CGTCGCAAGC ACTACAACAA CTGCAGAGAG GGCTGCGGCA 
GCCACCACAA AACAGGAGCT GGCCACCAAT GGATCGTCCA TATGGGACAT AAGAGCTTGG 
GCTAATAGTA ACTGGGCAAA CATCACCTGG AGCCACAATT ACTCTGCAGG AACTGACTTT 
GTGGTTAAGT ATATCACCAG TAACAAGACA GAGAAATCTA TCCCCGTTAA AGCTCAGACC 
CCCTCCTCTG TGCAGCTGGC CAATCTGACG CCGGGGATGG TGTACAAGCT GTGGGTGTTC 
CCCATATGGT CTAGCCCCAG CGAGCACTCG TACATAACCT TTACCACCAG CTCAGCTTAC 
ACCAAGAACC ACGTGGACAT CGCGACCCAG GGCTGGTTCA TCGGGCTGAT GTGTGCCATC 
GCCCTCCTGG TCCTCATTCT GCTGATTGTT TGCTTCATTA AGAGGAGCAG AGGAGGGAAA 
TACCCAGTGC GTGACAACAA AGATGAGCAC CTGAATCCTG AAGACAAGAA CGTAGAAGAT 
GGCTCATTCG ACTACAGGTC TCTTGAAAGC GATGAAGACA ACAAACCACT GCCCAACAGT 
CAGACCTCCC TGGATGGCAC GATAAAGCAA CAGGAGAGTG ACGACAGCTT GGTGGACTAC 
GGAGAGGGTG GGGAAGGGCA GTTCAACGAG GACGGCTCCT TCATTGGCCA GTACACAGTG 
AAAAAGGACA AGGAGGAAAC GGAAGGCAAC GAGAGCTCAG AAGCCACGTC CCCAGTCAAT 
GCTATCTACT CATTAGCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154395.1
CDS	169..4209
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTTRGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELATNGSSIWDIRAWANSNWANITWSHNYSAGTDFVVKYITSNKTEKSIPVKAQTPSSVQLANLTPGMVYKLWVFPIWSSPSEHSYITFTTSSAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298909.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298909.2

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA 
ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC 
CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG 
TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG 
GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC 
AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG 
CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA 
GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT 
TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG 
ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC 
AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC 
ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC 
CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG 
GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC 
ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA 
GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT 
CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG 
GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA 
TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC 
AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC 
TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG 
AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC 
GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT 
GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG 
ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC 
AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC 
AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT 
TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG 
GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG 
GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA 
GATTATCCCA AGGCTGCACC TACAGATGTT AGGATAAGAG TTTTAAACAG CACTGCCATT 
GCTCTGACAT GGACCCGCGT GCACCTGGAC ACCATCCAGG GACAGCTTAA GGAGTACAGA 
GCCTATTTCT GGAGAGACAG TAGTTTGCTG AAGAACCTGT GGGTCTCCAA AAAACGGCAG 
TATGTGAGTT TTCCTGGAGA CCGAAACCGG GGCATAGTGT CCCGGCTGTT CCCTTACAGC 
AACTACAAGC TTGAGATGGT TGTAACCAAC GGGAGAGGCG ATGGGCCCCG CAGCGAAGTC 
AAGGAGTTCC CCACACCTGA AGGAGTGCCC AGCTCCCCCA GGTACTTAAG AATCCGACAG 
CCAAATCTGG AAAGCATCAA TTTGGAGTGG GATCACCCAG AACATCCCAA TGGAGTCCTC 
ACGGGATACA ACCTTAGATA TCAAGCCTTT AACGGATCCA AAACGGGCAG AACCCTGGTA 
GAGAACTTCT CTCCCAACCA GACAAGGTTC ACCGTGCAGA GGACAGACCC CATCTCGCGC 
TATCGATTCT TCCTGCGTGC TCGGACACAG GTGGGAGATG GAGAAGTCAT AGTGGAAGAG 
TCACCAGCGC TCCTGAATGA AGCCACGCCA ACCCCAGCCT CCACCTGGTT GCCTCCACCT 
ACAACCGAGC TAACTCCAGC AGCCACCATT GCCACCACAA CCACCACTGC CACCCCTACT 
ACTGAAACCC CTCCTACTGA AATCCCTACT ACTGCCATCC CTACCACCAC CACTACTACA 
ACCACCACAG CTGCCAGCAC CGTCGCAAGC ACTACAACAA CTGCAGAGAG GGCTGCGGCA 
GCCACCACAA AACAGGAGCT GGCCACCAAT GGATCGTCCA TATGGGACAT AAGAGCTTGG 
GCTAATAGTA ACTGGGCAAA CATCACCTGG AGCCACAATT ACTCTGCAGG AACTGACTTT 
GTGGTTAAGT ATATCACCAG TAACAAGACA GAGAAATCTA TCCCCGTTAA AGCTCAGACC 
CCCTCCTCTG TGCAGCTGGC CAATCTGACG CCGGGGATGG TGTACAAGCT GTGGGTGTTC 
CCCATATGGT CTAGCCCCAG CGAGCACTCG TACATAACCT TTACCACCAG CTCAGCTTAC 
ACCAAGAACC ACGTGGACAT CGCGACCCAG GGCTGGTTCA TCGGGCTGAT GTGTGCCATC 
GCCCTCCTGG TCCTCATTCT GCTGATTGTT TGCTTCATTA AGAGGAGCAG AGGAGGGAAA 
TACCCAGTGC GTGACAACAA AGATGAGCAC CTGAATCCTG AAGACAAGAA CGTAGAAGAT 
GGCTCATTCG ACTACAGGTC TCTTGAAAGC GATGAAGACA ACAAACCACT GCCCAACAGT 
CAGACCTCCC TGGATGGCAC GATAAAGCAA CAGGAGAGTG ACGACAGCTT GGTGGACTAC 
GGAGAGGGTG GGGAAGGGCA GTTCAACGAG GACGGCTCCT TCATTGGCCA GTACACAGTG 
AAAAAGGACA AGGAGGAAAC GGAAGGCAAC GAGAGCTCAG AAGCCACGTC CCCAGTCAAT 
GCTATCTACT CATTAGCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298910.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154396.1 Gallus gallus neurofascin (NFASC), transcript variant X8, mRNA.

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CloneID	OGa46517
Clone ID Related Accession (Same CDS sequence)	XM_015298910.1 , XM_015298910.2
Accession Version	XM_015298910.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG 
CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC 
AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA 
AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG 
GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC 
ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG 
AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT 
CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG 
GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG 
GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG 
AAAAATCCCT ACACCCTCAA GGTGAAAACC ACCCGAGGGG TAACGGAAAC AACACCCAGC 
TTCATGTACC CATATGGGAC CTCCAGCAGC CAGATGGTGC TCCGAGGGGT GGACCTCTTG 
CTGGAGTGCA TTGCATCAGG AGTACCAGCA CCAGACATCA TGTGGTACAA GAAAGGAGGT 
GAGCTCCCAG CAGGCAAAAC CAAGCTGGAA AACTTTAACA AGGCCCTTCG TATCTCCAAC 
GTCTCGGAGG AAGACTCTGG GGAGTATTTC TGCCTGGCAT CCAACAAGAT GGGCAGCATC 
CGCCACACGA TCTCGGTGAG AGTGAAGGCT GCCCCGTATT GGCTGGATGA GCCACAGAAT 
CTCATTTTGG CCCCTGGTGA GGACGGCAGG TTGGTGTGTC GAGCCAATGG GAACCCCAAG 
CCTTCAATCC AGTGGTTGGT GAATGGAGAG CCCATTGAAG GTTCTCCACC CAACCCAAGC 
AGAGAGGTGG CTGGAGATAC CATTGTGTTT CGAGACACGC AGATCGGCAG CAGCGCTGTG 
TACCAATGCA ATGCTTCCAA CGAGCACGGC TACCTCCTTG CCAATGCCTT TGTCAGTGTC 
CTGGATGTGC CACCACGGAT ACTGGCCCCA CGCAACCAGC TCATCAAAGT CATCCAGTAC 
AACAGGACCC GGCTGGACTG CCCTTTTTTC GGCTCACCCA TCCCCACCCT GCGATGGTTT 
AAGAACGGCC AGGGGAACAT GCTGGATGGA GGGAACTACA AGGCGCATGA GAACGGGAGC 
TTGGAGATGA GCATGGCTCG GAAGGAGGAT CAGGGCATCT ACACCTGTGT CGCCACCAAC 
ATCCTGGGCA AAGTGGAGGC CCAGGTTCGC CTGGAAGTCA AAGACCCTAC CAGGATTGTG 
AGAGGCCCCG AAGATCAGGT GGTGAAGAGG GGCTCCATGC CTCGCCTGCA CTGCCGGGTG 
AAGCACGACC CAACACTGAA GCTCACGGTC ACCTGGCTGA AGGACGACGC TCCTCTCTAC 
ATTGGGAACA GGATGAAAAA AGAAGATGAT GGTCTGACAA TATATGGCGT GGCTGAGAAG 
GACCAGGGTG ACTACACCTG CGTGGCCAGC ACAGAGCTGG ACAAGGACTC AGCTAAAGCC 
TACCTCACCG TGCTAGCAAT CCCTGCTAAC CGTTTGAGAG ACTTACCTAA AGAGCGACCC 
GACCGGCCCC GGGACCTGGA GCTGTCAGAC CTGGCTGAGA GGAGCGTGAA GCTGACATGG 
ATTCCTGGCG ATGACAACAA CAGCCCCATC ACAGACTACA TCGTCCAGTT TGAGGAGGAC 
CGCTTCCAGC CTGGCACGTG GCACAACCAC TCCAGGTATC CTGGGAATGT CAACTCGGCC 
CTCCTGAGCC TCTCTCCTTA CGTCAACTAC CAATTTCGGG TGATTGCAGT GAACGACGTG 
GGCAGCAGCC TGCCCAGCAT GCCCTCAGAA CGATACCAGA CCAGCGGGGC ACGACCTGAA 
ATTAACCCAA CAGGAGTTCA AGGAGCAGGG ACCCAAAAAA ACAACATGGA GATAACCTGG 
ACGCCTCTGA ATGCAACTCA AGCCTATGGG CCCAACCTCC GTTACATCGT GCGGTGGAGG 
CGAAGGGACC CACGTGGGAG TTGGTACAAC GAGACGGTGA AGGCACCACG ACACGTCGTC 
TGGAACACAC CCATCTACGT CCCCTACGAG ATCAAAGTGC AGGCAGAGAA TGACTTTGGT 
AGAGCTCCAG AGCCTGAGAC CTACATCGGC TACTCAGGGG AAGATTATCC CAAGGCTGCA 
CCTACAGATG TTAGGATAAG AGTTTTAAAC AGCACTGCCA TTGCTCTGAC ATGGACCCGC 
GTGCACCTGG ACACCATCCA GGGACAGCTT AAGGAGTACA GAGCCTATTT CTGGAGAGAC 
AGTAGTTTGC TGAAGAACCT GTGGGTCTCC AAAAAACGGC AGTATGTGAG TTTTCCTGGA 
GACCGAAACC GGGGCATAGT GTCCCGGCTG TTCCCTTACA GCAACTACAA GCTTGAGATG 
GTTGTAACCA ACGGGAGAGG CGATGGGCCC CGCAGCGAAG TCAAGGAGTT CCCCACACCT 
GAAGGAGTGC CCAGCTCCCC CAGGTACTTA AGAATCCGAC AGCCAAATCT GGAAAGCATC 
AATTTGGAGT GGGATCACCC AGAACATCCC AATGGAGTCC TCACGGGATA CAACCTTAGA 
TATCAAGCCT TTAACGGATC CAAAACGGGC AGAACCCTGG TAGAGAACTT CTCTCCCAAC 
CAGACAAGGT TCACCGTGCA GAGGACAGAC CCCATCTCGC GCTATCGATT CTTCCTGCGT 
GCTCGGACAC AGGTGGGAGA TGGAGAAGTC ATAGTGGAAG AGTCACCAGC GCTCCTGAAT 
GAAGCCACGC CAACCCCAGC CTCCACCTGG TTGCCTCCAC CTACAACCGA GCTAACTCCA 
GCAGCCACCA TTGCCACCAC AACCACCACT GCCACCCCTA CTACTGAAAC CCCTCCTACT 
GAAATCCCTA CTACTGCCAT CCCTACCACC ACCACTACTA CAACCACCAC AGCTGCCAGC 
ACCGTCGCAA GCACTACAAC AACTGCAGAG AGGGCTGCGG CAGCCACCAC AAAACAGGAG 
CTGGCCACCA ATGGATCGTC CATATGGGAC ATAAGAGCTT GGGCTAATAG TAACTGGGCA 
AACATCACCT GGAGCCACAA TTACTCTGCA GGAACTGACT TTGTGGTTAA GTATATCACC 
AGTAACAAGA CAGAGAAATC TATCCCCGTT AAAGCTCAGA CCCCCTCCTC TGTGCAGCTG 
GCCAATCTGA CGCCGGGGAT GGTGTACAAG CTGTGGGTGT TCCCCATATG GTCTAGCCCC 
AGCGAGCACT CGTACATAAC CTTTACCACC AGCTCAGCTT ACACCAAGAA CCACGTGGAC 
ATCGCGACCC AGGGCTGGTT CATCGGGCTG ATGTGTGCCA TCGCCCTCCT GGTCCTCATT 
CTGCTGATTG TTTGCTTCAT TAAGAGGAGC AGAGGAGGGA AATACCCAGT GCGTGACAAC 
AAAGATGAGC ACCTGAATCC TGAAGACAAG AACGTAGAAG ATGGCTCATT CGACTACAGG 
TCTCTTGAAA GCGATGAAGA CAACAAACCA CTGCCCAACA GTCAGACCTC CCTGGATGGC 
ACGATAAAGC AACAGGAGAG TGACGACAGC TTGGTGGACT ACGGAGAGGG TGGGGAAGGG 
CAGTTCAACG AGGACGGCTC CTTCATTGGC CAGTACACAG TGAAAAAGGA CAAGGAGGAA 
ACGGAAGGCA ACGAGAGCTC AGAAGCCACG TCCCCAGTCA ATGCTATCTA CTCATTAGCG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154396.1
CDS	159..4181
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDLPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTTRGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELATNGSSIWDIRAWANSNWANITWSHNYSAGTDFVVKYITSNKTEKSIPVKAQTPSSVQLANLTPGMVYKLWVFPIWSSPSEHSYITFTTSSAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298910.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298910.1

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG 
CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC 
AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA 
AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG 
GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC 
ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG 
AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT 
CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG 
GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG 
GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG 
AAAAATCCCT ACACCCTCAA GGTGAAAACC ACCCGAGGGG TAACGGAAAC AACACCCAGC 
TTCATGTACC CATATGGGAC CTCCAGCAGC CAGATGGTGC TCCGAGGGGT GGACCTCTTG 
CTGGAGTGCA TTGCATCAGG AGTACCAGCA CCAGACATCA TGTGGTACAA GAAAGGAGGT 
GAGCTCCCAG CAGGCAAAAC CAAGCTGGAA AACTTTAACA AGGCCCTTCG TATCTCCAAC 
GTCTCGGAGG AAGACTCTGG GGAGTATTTC TGCCTGGCAT CCAACAAGAT GGGCAGCATC 
CGCCACACGA TCTCGGTGAG AGTGAAGGCT GCCCCGTATT GGCTGGATGA GCCACAGAAT 
CTCATTTTGG CCCCTGGTGA GGACGGCAGG TTGGTGTGTC GAGCCAATGG GAACCCCAAG 
CCTTCAATCC AGTGGTTGGT GAATGGAGAG CCCATTGAAG GTTCTCCACC CAACCCAAGC 
AGAGAGGTGG CTGGAGATAC CATTGTGTTT CGAGACACGC AGATCGGCAG CAGCGCTGTG 
TACCAATGCA ATGCTTCCAA CGAGCACGGC TACCTCCTTG CCAATGCCTT TGTCAGTGTC 
CTGGATGTGC CACCACGGAT ACTGGCCCCA CGCAACCAGC TCATCAAAGT CATCCAGTAC 
AACAGGACCC GGCTGGACTG CCCTTTTTTC GGCTCACCCA TCCCCACCCT GCGATGGTTT 
AAGAACGGCC AGGGGAACAT GCTGGATGGA GGGAACTACA AGGCGCATGA GAACGGGAGC 
TTGGAGATGA GCATGGCTCG GAAGGAGGAT CAGGGCATCT ACACCTGTGT CGCCACCAAC 
ATCCTGGGCA AAGTGGAGGC CCAGGTTCGC CTGGAAGTCA AAGACCCTAC CAGGATTGTG 
AGAGGCCCCG AAGATCAGGT GGTGAAGAGG GGCTCCATGC CTCGCCTGCA CTGCCGGGTG 
AAGCACGACC CAACACTGAA GCTCACGGTC ACCTGGCTGA AGGACGACGC TCCTCTCTAC 
ATTGGGAACA GGATGAAAAA AGAAGATGAT GGTCTGACAA TATATGGCGT GGCTGAGAAG 
GACCAGGGTG ACTACACCTG CGTGGCCAGC ACAGAGCTGG ACAAGGACTC AGCTAAAGCC 
TACCTCACCG TGCTAGCAAT CCCTGCTAAC CGTTTGAGAG ACTTACCTAA AGAGCGACCC 
GACCGGCCCC GGGACCTGGA GCTGTCAGAC CTGGCTGAGA GGAGCGTGAA GCTGACATGG 
ATTCCTGGCG ATGACAACAA CAGCCCCATC ACAGACTACA TCGTCCAGTT TGAGGAGGAC 
CGCTTCCAGC CTGGCACGTG GCACAACCAC TCCAGGTATC CTGGGAATGT CAACTCGGCC 
CTCCTGAGCC TCTCTCCTTA CGTCAACTAC CAATTTCGGG TGATTGCAGT GAACGACGTG 
GGCAGCAGCC TGCCCAGCAT GCCCTCAGAA CGATACCAGA CCAGCGGGGC ACGACCTGAA 
ATTAACCCAA CAGGAGTTCA AGGAGCAGGG ACCCAAAAAA ACAACATGGA GATAACCTGG 
ACGCCTCTGA ATGCAACTCA AGCCTATGGG CCCAACCTCC GTTACATCGT GCGGTGGAGG 
CGAAGGGACC CACGTGGGAG TTGGTACAAC GAGACGGTGA AGGCACCACG ACACGTCGTC 
TGGAACACAC CCATCTACGT CCCCTACGAG ATCAAAGTGC AGGCAGAGAA TGACTTTGGT 
AGAGCTCCAG AGCCTGAGAC CTACATCGGC TACTCAGGGG AAGATTATCC CAAGGCTGCA 
CCTACAGATG TTAGGATAAG AGTTTTAAAC AGCACTGCCA TTGCTCTGAC ATGGACCCGC 
GTGCACCTGG ACACCATCCA GGGACAGCTT AAGGAGTACA GAGCCTATTT CTGGAGAGAC 
AGTAGTTTGC TGAAGAACCT GTGGGTCTCC AAAAAACGGC AGTATGTGAG TTTTCCTGGA 
GACCGAAACC GGGGCATAGT GTCCCGGCTG TTCCCTTACA GCAACTACAA GCTTGAGATG 
GTTGTAACCA ACGGGAGAGG CGATGGGCCC CGCAGCGAAG TCAAGGAGTT CCCCACACCT 
GAAGGAGTGC CCAGCTCCCC CAGGTACTTA AGAATCCGAC AGCCAAATCT GGAAAGCATC 
AATTTGGAGT GGGATCACCC AGAACATCCC AATGGAGTCC TCACGGGATA CAACCTTAGA 
TATCAAGCCT TTAACGGATC CAAAACGGGC AGAACCCTGG TAGAGAACTT CTCTCCCAAC 
CAGACAAGGT TCACCGTGCA GAGGACAGAC CCCATCTCGC GCTATCGATT CTTCCTGCGT 
GCTCGGACAC AGGTGGGAGA TGGAGAAGTC ATAGTGGAAG AGTCACCAGC GCTCCTGAAT 
GAAGCCACGC CAACCCCAGC CTCCACCTGG TTGCCTCCAC CTACAACCGA GCTAACTCCA 
GCAGCCACCA TTGCCACCAC AACCACCACT GCCACCCCTA CTACTGAAAC CCCTCCTACT 
GAAATCCCTA CTACTGCCAT CCCTACCACC ACCACTACTA CAACCACCAC AGCTGCCAGC 
ACCGTCGCAA GCACTACAAC AACTGCAGAG AGGGCTGCGG CAGCCACCAC AAAACAGGAG 
CTGGCCACCA ATGGATCGTC CATATGGGAC ATAAGAGCTT GGGCTAATAG TAACTGGGCA 
AACATCACCT GGAGCCACAA TTACTCTGCA GGAACTGACT TTGTGGTTAA GTATATCACC 
AGTAACAAGA CAGAGAAATC TATCCCCGTT AAAGCTCAGA CCCCCTCCTC TGTGCAGCTG 
GCCAATCTGA CGCCGGGGAT GGTGTACAAG CTGTGGGTGT TCCCCATATG GTCTAGCCCC 
AGCGAGCACT CGTACATAAC CTTTACCACC AGCTCAGCTT ACACCAAGAA CCACGTGGAC 
ATCGCGACCC AGGGCTGGTT CATCGGGCTG ATGTGTGCCA TCGCCCTCCT GGTCCTCATT 
CTGCTGATTG TTTGCTTCAT TAAGAGGAGC AGAGGAGGGA AATACCCAGT GCGTGACAAC 
AAAGATGAGC ACCTGAATCC TGAAGACAAG AACGTAGAAG ATGGCTCATT CGACTACAGG 
TCTCTTGAAA GCGATGAAGA CAACAAACCA CTGCCCAACA GTCAGACCTC CCTGGATGGC 
ACGATAAAGC AACAGGAGAG TGACGACAGC TTGGTGGACT ACGGAGAGGG TGGGGAAGGG 
CAGTTCAACG AGGACGGCTC CTTCATTGGC CAGTACACAG TGAAAAAGGA CAAGGAGGAA 
ACGGAAGGCA ACGAGAGCTC AGAAGCCACG TCCCCAGTCA ATGCTATCTA CTCATTAGCG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298910.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154396.1 Gallus gallus neurofascin (NFASC), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OGa46517
Clone ID Related Accession (Same CDS sequence)	XM_015298910.1 , XM_015298910.2
Accession Version	XM_015298910.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298910.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG 
CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC 
AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA 
AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG 
GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC 
ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG 
AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT 
CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG 
GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG 
GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG 
AAAAATCCCT ACACCCTCAA GGTGAAAACC ACCCGAGGGG TAACGGAAAC AACACCCAGC 
TTCATGTACC CATATGGGAC CTCCAGCAGC CAGATGGTGC TCCGAGGGGT GGACCTCTTG 
CTGGAGTGCA TTGCATCAGG AGTACCAGCA CCAGACATCA TGTGGTACAA GAAAGGAGGT 
GAGCTCCCAG CAGGCAAAAC CAAGCTGGAA AACTTTAACA AGGCCCTTCG TATCTCCAAC 
GTCTCGGAGG AAGACTCTGG GGAGTATTTC TGCCTGGCAT CCAACAAGAT GGGCAGCATC 
CGCCACACGA TCTCGGTGAG AGTGAAGGCT GCCCCGTATT GGCTGGATGA GCCACAGAAT 
CTCATTTTGG CCCCTGGTGA GGACGGCAGG TTGGTGTGTC GAGCCAATGG GAACCCCAAG 
CCTTCAATCC AGTGGTTGGT GAATGGAGAG CCCATTGAAG GTTCTCCACC CAACCCAAGC 
AGAGAGGTGG CTGGAGATAC CATTGTGTTT CGAGACACGC AGATCGGCAG CAGCGCTGTG 
TACCAATGCA ATGCTTCCAA CGAGCACGGC TACCTCCTTG CCAATGCCTT TGTCAGTGTC 
CTGGATGTGC CACCACGGAT ACTGGCCCCA CGCAACCAGC TCATCAAAGT CATCCAGTAC 
AACAGGACCC GGCTGGACTG CCCTTTTTTC GGCTCACCCA TCCCCACCCT GCGATGGTTT 
AAGAACGGCC AGGGGAACAT GCTGGATGGA GGGAACTACA AGGCGCATGA GAACGGGAGC 
TTGGAGATGA GCATGGCTCG GAAGGAGGAT CAGGGCATCT ACACCTGTGT CGCCACCAAC 
ATCCTGGGCA AAGTGGAGGC CCAGGTTCGC CTGGAAGTCA AAGACCCTAC CAGGATTGTG 
AGAGGCCCCG AAGATCAGGT GGTGAAGAGG GGCTCCATGC CTCGCCTGCA CTGCCGGGTG 
AAGCACGACC CAACACTGAA GCTCACGGTC ACCTGGCTGA AGGACGACGC TCCTCTCTAC 
ATTGGGAACA GGATGAAAAA AGAAGATGAT GGTCTGACAA TATATGGCGT GGCTGAGAAG 
GACCAGGGTG ACTACACCTG CGTGGCCAGC ACAGAGCTGG ACAAGGACTC AGCTAAAGCC 
TACCTCACCG TGCTAGCAAT CCCTGCTAAC CGTTTGAGAG ACTTACCTAA AGAGCGACCC 
GACCGGCCCC GGGACCTGGA GCTGTCAGAC CTGGCTGAGA GGAGCGTGAA GCTGACATGG 
ATTCCTGGCG ATGACAACAA CAGCCCCATC ACAGACTACA TCGTCCAGTT TGAGGAGGAC 
CGCTTCCAGC CTGGCACGTG GCACAACCAC TCCAGGTATC CTGGGAATGT CAACTCGGCC 
CTCCTGAGCC TCTCTCCTTA CGTCAACTAC CAATTTCGGG TGATTGCAGT GAACGACGTG 
GGCAGCAGCC TGCCCAGCAT GCCCTCAGAA CGATACCAGA CCAGCGGGGC ACGACCTGAA 
ATTAACCCAA CAGGAGTTCA AGGAGCAGGG ACCCAAAAAA ACAACATGGA GATAACCTGG 
ACGCCTCTGA ATGCAACTCA AGCCTATGGG CCCAACCTCC GTTACATCGT GCGGTGGAGG 
CGAAGGGACC CACGTGGGAG TTGGTACAAC GAGACGGTGA AGGCACCACG ACACGTCGTC 
TGGAACACAC CCATCTACGT CCCCTACGAG ATCAAAGTGC AGGCAGAGAA TGACTTTGGT 
AGAGCTCCAG AGCCTGAGAC CTACATCGGC TACTCAGGGG AAGATTATCC CAAGGCTGCA 
CCTACAGATG TTAGGATAAG AGTTTTAAAC AGCACTGCCA TTGCTCTGAC ATGGACCCGC 
GTGCACCTGG ACACCATCCA GGGACAGCTT AAGGAGTACA GAGCCTATTT CTGGAGAGAC 
AGTAGTTTGC TGAAGAACCT GTGGGTCTCC AAAAAACGGC AGTATGTGAG TTTTCCTGGA 
GACCGAAACC GGGGCATAGT GTCCCGGCTG TTCCCTTACA GCAACTACAA GCTTGAGATG 
GTTGTAACCA ACGGGAGAGG CGATGGGCCC CGCAGCGAAG TCAAGGAGTT CCCCACACCT 
GAAGGAGTGC CCAGCTCCCC CAGGTACTTA AGAATCCGAC AGCCAAATCT GGAAAGCATC 
AATTTGGAGT GGGATCACCC AGAACATCCC AATGGAGTCC TCACGGGATA CAACCTTAGA 
TATCAAGCCT TTAACGGATC CAAAACGGGC AGAACCCTGG TAGAGAACTT CTCTCCCAAC 
CAGACAAGGT TCACCGTGCA GAGGACAGAC CCCATCTCGC GCTATCGATT CTTCCTGCGT 
GCTCGGACAC AGGTGGGAGA TGGAGAAGTC ATAGTGGAAG AGTCACCAGC GCTCCTGAAT 
GAAGCCACGC CAACCCCAGC CTCCACCTGG TTGCCTCCAC CTACAACCGA GCTAACTCCA 
GCAGCCACCA TTGCCACCAC AACCACCACT GCCACCCCTA CTACTGAAAC CCCTCCTACT 
GAAATCCCTA CTACTGCCAT CCCTACCACC ACCACTACTA CAACCACCAC AGCTGCCAGC 
ACCGTCGCAA GCACTACAAC AACTGCAGAG AGGGCTGCGG CAGCCACCAC AAAACAGGAG 
CTGGCCACCA ATGGATCGTC CATATGGGAC ATAAGAGCTT GGGCTAATAG TAACTGGGCA 
AACATCACCT GGAGCCACAA TTACTCTGCA GGAACTGACT TTGTGGTTAA GTATATCACC 
AGTAACAAGA CAGAGAAATC TATCCCCGTT AAAGCTCAGA CCCCCTCCTC TGTGCAGCTG 
GCCAATCTGA CGCCGGGGAT GGTGTACAAG CTGTGGGTGT TCCCCATATG GTCTAGCCCC 
AGCGAGCACT CGTACATAAC CTTTACCACC AGCTCAGCTT ACACCAAGAA CCACGTGGAC 
ATCGCGACCC AGGGCTGGTT CATCGGGCTG ATGTGTGCCA TCGCCCTCCT GGTCCTCATT 
CTGCTGATTG TTTGCTTCAT TAAGAGGAGC AGAGGAGGGA AATACCCAGT GCGTGACAAC 
AAAGATGAGC ACCTGAATCC TGAAGACAAG AACGTAGAAG ATGGCTCATT CGACTACAGG 
TCTCTTGAAA GCGATGAAGA CAACAAACCA CTGCCCAACA GTCAGACCTC CCTGGATGGC 
ACGATAAAGC AACAGGAGAG TGACGACAGC TTGGTGGACT ACGGAGAGGG TGGGGAAGGG 
CAGTTCAACG AGGACGGCTC CTTCATTGGC CAGTACACAG TGAAAAAGGA CAAGGAGGAA 
ACGGAAGGCA ACGAGAGCTC AGAAGCCACG TCCCCAGTCA ATGCTATCTA CTCATTAGCG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154396.1
CDS	169..4191
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDLPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTTRGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELATNGSSIWDIRAWANSNWANITWSHNYSAGTDFVVKYITSNKTEKSIPVKAQTPSSVQLANLTPGMVYKLWVFPIWSSPSEHSYITFTTSSAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298910.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298910.2

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG 
CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC 
AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA 
AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG 
GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC 
ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG 
AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT 
CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG 
GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG 
GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG 
AAAAATCCCT ACACCCTCAA GGTGAAAACC ACCCGAGGGG TAACGGAAAC AACACCCAGC 
TTCATGTACC CATATGGGAC CTCCAGCAGC CAGATGGTGC TCCGAGGGGT GGACCTCTTG 
CTGGAGTGCA TTGCATCAGG AGTACCAGCA CCAGACATCA TGTGGTACAA GAAAGGAGGT 
GAGCTCCCAG CAGGCAAAAC CAAGCTGGAA AACTTTAACA AGGCCCTTCG TATCTCCAAC 
GTCTCGGAGG AAGACTCTGG GGAGTATTTC TGCCTGGCAT CCAACAAGAT GGGCAGCATC 
CGCCACACGA TCTCGGTGAG AGTGAAGGCT GCCCCGTATT GGCTGGATGA GCCACAGAAT 
CTCATTTTGG CCCCTGGTGA GGACGGCAGG TTGGTGTGTC GAGCCAATGG GAACCCCAAG 
CCTTCAATCC AGTGGTTGGT GAATGGAGAG CCCATTGAAG GTTCTCCACC CAACCCAAGC 
AGAGAGGTGG CTGGAGATAC CATTGTGTTT CGAGACACGC AGATCGGCAG CAGCGCTGTG 
TACCAATGCA ATGCTTCCAA CGAGCACGGC TACCTCCTTG CCAATGCCTT TGTCAGTGTC 
CTGGATGTGC CACCACGGAT ACTGGCCCCA CGCAACCAGC TCATCAAAGT CATCCAGTAC 
AACAGGACCC GGCTGGACTG CCCTTTTTTC GGCTCACCCA TCCCCACCCT GCGATGGTTT 
AAGAACGGCC AGGGGAACAT GCTGGATGGA GGGAACTACA AGGCGCATGA GAACGGGAGC 
TTGGAGATGA GCATGGCTCG GAAGGAGGAT CAGGGCATCT ACACCTGTGT CGCCACCAAC 
ATCCTGGGCA AAGTGGAGGC CCAGGTTCGC CTGGAAGTCA AAGACCCTAC CAGGATTGTG 
AGAGGCCCCG AAGATCAGGT GGTGAAGAGG GGCTCCATGC CTCGCCTGCA CTGCCGGGTG 
AAGCACGACC CAACACTGAA GCTCACGGTC ACCTGGCTGA AGGACGACGC TCCTCTCTAC 
ATTGGGAACA GGATGAAAAA AGAAGATGAT GGTCTGACAA TATATGGCGT GGCTGAGAAG 
GACCAGGGTG ACTACACCTG CGTGGCCAGC ACAGAGCTGG ACAAGGACTC AGCTAAAGCC 
TACCTCACCG TGCTAGCAAT CCCTGCTAAC CGTTTGAGAG ACTTACCTAA AGAGCGACCC 
GACCGGCCCC GGGACCTGGA GCTGTCAGAC CTGGCTGAGA GGAGCGTGAA GCTGACATGG 
ATTCCTGGCG ATGACAACAA CAGCCCCATC ACAGACTACA TCGTCCAGTT TGAGGAGGAC 
CGCTTCCAGC CTGGCACGTG GCACAACCAC TCCAGGTATC CTGGGAATGT CAACTCGGCC 
CTCCTGAGCC TCTCTCCTTA CGTCAACTAC CAATTTCGGG TGATTGCAGT GAACGACGTG 
GGCAGCAGCC TGCCCAGCAT GCCCTCAGAA CGATACCAGA CCAGCGGGGC ACGACCTGAA 
ATTAACCCAA CAGGAGTTCA AGGAGCAGGG ACCCAAAAAA ACAACATGGA GATAACCTGG 
ACGCCTCTGA ATGCAACTCA AGCCTATGGG CCCAACCTCC GTTACATCGT GCGGTGGAGG 
CGAAGGGACC CACGTGGGAG TTGGTACAAC GAGACGGTGA AGGCACCACG ACACGTCGTC 
TGGAACACAC CCATCTACGT CCCCTACGAG ATCAAAGTGC AGGCAGAGAA TGACTTTGGT 
AGAGCTCCAG AGCCTGAGAC CTACATCGGC TACTCAGGGG AAGATTATCC CAAGGCTGCA 
CCTACAGATG TTAGGATAAG AGTTTTAAAC AGCACTGCCA TTGCTCTGAC ATGGACCCGC 
GTGCACCTGG ACACCATCCA GGGACAGCTT AAGGAGTACA GAGCCTATTT CTGGAGAGAC 
AGTAGTTTGC TGAAGAACCT GTGGGTCTCC AAAAAACGGC AGTATGTGAG TTTTCCTGGA 
GACCGAAACC GGGGCATAGT GTCCCGGCTG TTCCCTTACA GCAACTACAA GCTTGAGATG 
GTTGTAACCA ACGGGAGAGG CGATGGGCCC CGCAGCGAAG TCAAGGAGTT CCCCACACCT 
GAAGGAGTGC CCAGCTCCCC CAGGTACTTA AGAATCCGAC AGCCAAATCT GGAAAGCATC 
AATTTGGAGT GGGATCACCC AGAACATCCC AATGGAGTCC TCACGGGATA CAACCTTAGA 
TATCAAGCCT TTAACGGATC CAAAACGGGC AGAACCCTGG TAGAGAACTT CTCTCCCAAC 
CAGACAAGGT TCACCGTGCA GAGGACAGAC CCCATCTCGC GCTATCGATT CTTCCTGCGT 
GCTCGGACAC AGGTGGGAGA TGGAGAAGTC ATAGTGGAAG AGTCACCAGC GCTCCTGAAT 
GAAGCCACGC CAACCCCAGC CTCCACCTGG TTGCCTCCAC CTACAACCGA GCTAACTCCA 
GCAGCCACCA TTGCCACCAC AACCACCACT GCCACCCCTA CTACTGAAAC CCCTCCTACT 
GAAATCCCTA CTACTGCCAT CCCTACCACC ACCACTACTA CAACCACCAC AGCTGCCAGC 
ACCGTCGCAA GCACTACAAC AACTGCAGAG AGGGCTGCGG CAGCCACCAC AAAACAGGAG 
CTGGCCACCA ATGGATCGTC CATATGGGAC ATAAGAGCTT GGGCTAATAG TAACTGGGCA 
AACATCACCT GGAGCCACAA TTACTCTGCA GGAACTGACT TTGTGGTTAA GTATATCACC 
AGTAACAAGA CAGAGAAATC TATCCCCGTT AAAGCTCAGA CCCCCTCCTC TGTGCAGCTG 
GCCAATCTGA CGCCGGGGAT GGTGTACAAG CTGTGGGTGT TCCCCATATG GTCTAGCCCC 
AGCGAGCACT CGTACATAAC CTTTACCACC AGCTCAGCTT ACACCAAGAA CCACGTGGAC 
ATCGCGACCC AGGGCTGGTT CATCGGGCTG ATGTGTGCCA TCGCCCTCCT GGTCCTCATT 
CTGCTGATTG TTTGCTTCAT TAAGAGGAGC AGAGGAGGGA AATACCCAGT GCGTGACAAC 
AAAGATGAGC ACCTGAATCC TGAAGACAAG AACGTAGAAG ATGGCTCATT CGACTACAGG 
TCTCTTGAAA GCGATGAAGA CAACAAACCA CTGCCCAACA GTCAGACCTC CCTGGATGGC 
ACGATAAAGC AACAGGAGAG TGACGACAGC TTGGTGGACT ACGGAGAGGG TGGGGAAGGG 
CAGTTCAACG AGGACGGCTC CTTCATTGGC CAGTACACAG TGAAAAAGGA CAAGGAGGAA 
ACGGAAGGCA ACGAGAGCTC AGAAGCCACG TCCCCAGTCA ATGCTATCTA CTCATTAGCG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298911.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154397.1 Gallus gallus neurofascin (NFASC), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OGa46518
Clone ID Related Accession (Same CDS sequence)	XM_015298911.2 , XM_015298911.1
Accession Version	XM_015298911.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298911.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTTGCCC 
AGCTCCCCCA GGTACTTAAG AATCCGACAG CCAAATCTGG AAAGCATCAA TTTGGAGTGG 
GATCACCCAG AACATCCCAA TGGAGTCCTC ACGGGATACA ACCTTAGATA TCAAGCCTTT 
AACGGATCCA AAACGGGCAG AACCCTGGTA GAGAACTTCT CTCCCAACCA GACAAGGTTC 
ACCGTGCAGA GGACAGACCC CATCTCGCGC TATCGATTCT TCCTGCGTGC TCGGACACAG 
GTGGGAGATG GAGAAGTCAT AGTGGAAGAG TCACCAGCGC TCCTGAATGA AGCCACGCCA 
ACCCCAGCCT CCACCTGGTT GCCTCCACCT ACAACCGAGC TAACTCCAGC AGCCACCATT 
GCCACCACAA CCACCACTGC CACCCCTACT ACTGAAACCC CTCCTACTGA AATCCCTACT 
ACTGCCATCC CTACCACCAC CACTACTACA ACCACCACAG CTGCCAGCAC CGTCGCAAGC 
ACTACAACAA CTGCAGAGAG GGCTGCGGCA GCCACCACAA AACAGGAGCT GGCCACCAAT 
GGATCGTCCA TATGGGACAT AAGAGCTTGG GCTAATAGTA ACTGGGCAAA CATCACCTGG 
AGCCACAATT ACTCTGCAGG AACTGACTTT GTGGTTAAGT ATATCACCAG TAACAAGACA 
GAGAAATCTA TCCCCGTTAA AGCTCAGACC CCCTCCTCTG TGCAGCTGGC CAATCTGACG 
CCGGGGATGG TGTACAAGCT GTGGGTGTTC CCCATATGGT CTAGCCCCAG CGAGCACTCG 
TACATAACCT TTACCACCAG CTCAGCTTAC ACCAAGAACC ACGTGGACAT CGCGACCCAG 
GGCTGGTTCA TCGGGCTGAT GTGTGCCATC GCCCTCCTGG TCCTCATTCT GCTGATTGTT 
TGCTTCATTA AGAGGAGCAG AGGAGGGAAA TACCCAGTGC GTGACAACAA AGATGAGCAC 
CTGAATCCTG AAGACAAGAA CGTAGAAGAT GGCTCATTCG ACTACAGGTC TCTTGAAAGC 
GATGAAGACA ACAAACCACT GCCCAACAGT CAGACCTCCC TGGATGGCAC GATAAAGCAA 
CAGGAGAGTG ACGACAGCTT GGTGGACTAC GGAGAGGGTG GGGAAGGGCA GTTCAACGAG 
GACGGCTCCT TCATTGGCCA GTACACAGTG AAAAAGGACA AGGAGGAAAC GGAAGGCAAC 
GAGAGCTCAG AAGCCACGTC CCCAGTCAAT GCTATCTACT CATTAGCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154397.1
CDS	169..3939
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDLPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELATNGSSIWDIRAWANSNWANITWSHNYSAGTDFVVKYITSNKTEKSIPVKAQTPSSVQLANLTPGMVYKLWVFPIWSSPSEHSYITFTTSSAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298911.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298911.2

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTTGCCC 
AGCTCCCCCA GGTACTTAAG AATCCGACAG CCAAATCTGG AAAGCATCAA TTTGGAGTGG 
GATCACCCAG AACATCCCAA TGGAGTCCTC ACGGGATACA ACCTTAGATA TCAAGCCTTT 
AACGGATCCA AAACGGGCAG AACCCTGGTA GAGAACTTCT CTCCCAACCA GACAAGGTTC 
ACCGTGCAGA GGACAGACCC CATCTCGCGC TATCGATTCT TCCTGCGTGC TCGGACACAG 
GTGGGAGATG GAGAAGTCAT AGTGGAAGAG TCACCAGCGC TCCTGAATGA AGCCACGCCA 
ACCCCAGCCT CCACCTGGTT GCCTCCACCT ACAACCGAGC TAACTCCAGC AGCCACCATT 
GCCACCACAA CCACCACTGC CACCCCTACT ACTGAAACCC CTCCTACTGA AATCCCTACT 
ACTGCCATCC CTACCACCAC CACTACTACA ACCACCACAG CTGCCAGCAC CGTCGCAAGC 
ACTACAACAA CTGCAGAGAG GGCTGCGGCA GCCACCACAA AACAGGAGCT GGCCACCAAT 
GGATCGTCCA TATGGGACAT AAGAGCTTGG GCTAATAGTA ACTGGGCAAA CATCACCTGG 
AGCCACAATT ACTCTGCAGG AACTGACTTT GTGGTTAAGT ATATCACCAG TAACAAGACA 
GAGAAATCTA TCCCCGTTAA AGCTCAGACC CCCTCCTCTG TGCAGCTGGC CAATCTGACG 
CCGGGGATGG TGTACAAGCT GTGGGTGTTC CCCATATGGT CTAGCCCCAG CGAGCACTCG 
TACATAACCT TTACCACCAG CTCAGCTTAC ACCAAGAACC ACGTGGACAT CGCGACCCAG 
GGCTGGTTCA TCGGGCTGAT GTGTGCCATC GCCCTCCTGG TCCTCATTCT GCTGATTGTT 
TGCTTCATTA AGAGGAGCAG AGGAGGGAAA TACCCAGTGC GTGACAACAA AGATGAGCAC 
CTGAATCCTG AAGACAAGAA CGTAGAAGAT GGCTCATTCG ACTACAGGTC TCTTGAAAGC 
GATGAAGACA ACAAACCACT GCCCAACAGT CAGACCTCCC TGGATGGCAC GATAAAGCAA 
CAGGAGAGTG ACGACAGCTT GGTGGACTAC GGAGAGGGTG GGGAAGGGCA GTTCAACGAG 
GACGGCTCCT TCATTGGCCA GTACACAGTG AAAAAGGACA AGGAGGAAAC GGAAGGCAAC 
GAGAGCTCAG AAGCCACGTC CCCAGTCAAT GCTATCTACT CATTAGCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298911.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154397.1 Gallus gallus neurofascin (NFASC), transcript variant X9, mRNA.

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CloneID	OGa46518
Clone ID Related Accession (Same CDS sequence)	XM_015298911.2 , XM_015298911.1
Accession Version	XM_015298911.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTTGCCC 
AGCTCCCCCA GGTACTTAAG AATCCGACAG CCAAATCTGG AAAGCATCAA TTTGGAGTGG 
GATCACCCAG AACATCCCAA TGGAGTCCTC ACGGGATACA ACCTTAGATA TCAAGCCTTT 
AACGGATCCA AAACGGGCAG AACCCTGGTA GAGAACTTCT CTCCCAACCA GACAAGGTTC 
ACCGTGCAGA GGACAGACCC CATCTCGCGC TATCGATTCT TCCTGCGTGC TCGGACACAG 
GTGGGAGATG GAGAAGTCAT AGTGGAAGAG TCACCAGCGC TCCTGAATGA AGCCACGCCA 
ACCCCAGCCT CCACCTGGTT GCCTCCACCT ACAACCGAGC TAACTCCAGC AGCCACCATT 
GCCACCACAA CCACCACTGC CACCCCTACT ACTGAAACCC CTCCTACTGA AATCCCTACT 
ACTGCCATCC CTACCACCAC CACTACTACA ACCACCACAG CTGCCAGCAC CGTCGCAAGC 
ACTACAACAA CTGCAGAGAG GGCTGCGGCA GCCACCACAA AACAGGAGCT GGCCACCAAT 
GGATCGTCCA TATGGGACAT AAGAGCTTGG GCTAATAGTA ACTGGGCAAA CATCACCTGG 
AGCCACAATT ACTCTGCAGG AACTGACTTT GTGGTTAAGT ATATCACCAG TAACAAGACA 
GAGAAATCTA TCCCCGTTAA AGCTCAGACC CCCTCCTCTG TGCAGCTGGC CAATCTGACG 
CCGGGGATGG TGTACAAGCT GTGGGTGTTC CCCATATGGT CTAGCCCCAG CGAGCACTCG 
TACATAACCT TTACCACCAG CTCAGCTTAC ACCAAGAACC ACGTGGACAT CGCGACCCAG 
GGCTGGTTCA TCGGGCTGAT GTGTGCCATC GCCCTCCTGG TCCTCATTCT GCTGATTGTT 
TGCTTCATTA AGAGGAGCAG AGGAGGGAAA TACCCAGTGC GTGACAACAA AGATGAGCAC 
CTGAATCCTG AAGACAAGAA CGTAGAAGAT GGCTCATTCG ACTACAGGTC TCTTGAAAGC 
GATGAAGACA ACAAACCACT GCCCAACAGT CAGACCTCCC TGGATGGCAC GATAAAGCAA 
CAGGAGAGTG ACGACAGCTT GGTGGACTAC GGAGAGGGTG GGGAAGGGCA GTTCAACGAG 
GACGGCTCCT TCATTGGCCA GTACACAGTG AAAAAGGACA AGGAGGAAAC GGAAGGCAAC 
GAGAGCTCAG AAGCCACGTC CCCAGTCAAT GCTATCTACT CATTAGCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154397.1
CDS	159..3929
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDLPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELATNGSSIWDIRAWANSNWANITWSHNYSAGTDFVVKYITSNKTEKSIPVKAQTPSSVQLANLTPGMVYKLWVFPIWSSPSEHSYITFTTSSAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298911.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298911.1

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTTGCCC 
AGCTCCCCCA GGTACTTAAG AATCCGACAG CCAAATCTGG AAAGCATCAA TTTGGAGTGG 
GATCACCCAG AACATCCCAA TGGAGTCCTC ACGGGATACA ACCTTAGATA TCAAGCCTTT 
AACGGATCCA AAACGGGCAG AACCCTGGTA GAGAACTTCT CTCCCAACCA GACAAGGTTC 
ACCGTGCAGA GGACAGACCC CATCTCGCGC TATCGATTCT TCCTGCGTGC TCGGACACAG 
GTGGGAGATG GAGAAGTCAT AGTGGAAGAG TCACCAGCGC TCCTGAATGA AGCCACGCCA 
ACCCCAGCCT CCACCTGGTT GCCTCCACCT ACAACCGAGC TAACTCCAGC AGCCACCATT 
GCCACCACAA CCACCACTGC CACCCCTACT ACTGAAACCC CTCCTACTGA AATCCCTACT 
ACTGCCATCC CTACCACCAC CACTACTACA ACCACCACAG CTGCCAGCAC CGTCGCAAGC 
ACTACAACAA CTGCAGAGAG GGCTGCGGCA GCCACCACAA AACAGGAGCT GGCCACCAAT 
GGATCGTCCA TATGGGACAT AAGAGCTTGG GCTAATAGTA ACTGGGCAAA CATCACCTGG 
AGCCACAATT ACTCTGCAGG AACTGACTTT GTGGTTAAGT ATATCACCAG TAACAAGACA 
GAGAAATCTA TCCCCGTTAA AGCTCAGACC CCCTCCTCTG TGCAGCTGGC CAATCTGACG 
CCGGGGATGG TGTACAAGCT GTGGGTGTTC CCCATATGGT CTAGCCCCAG CGAGCACTCG 
TACATAACCT TTACCACCAG CTCAGCTTAC ACCAAGAACC ACGTGGACAT CGCGACCCAG 
GGCTGGTTCA TCGGGCTGAT GTGTGCCATC GCCCTCCTGG TCCTCATTCT GCTGATTGTT 
TGCTTCATTA AGAGGAGCAG AGGAGGGAAA TACCCAGTGC GTGACAACAA AGATGAGCAC 
CTGAATCCTG AAGACAAGAA CGTAGAAGAT GGCTCATTCG ACTACAGGTC TCTTGAAAGC 
GATGAAGACA ACAAACCACT GCCCAACAGT CAGACCTCCC TGGATGGCAC GATAAAGCAA 
CAGGAGAGTG ACGACAGCTT GGTGGACTAC GGAGAGGGTG GGGAAGGGCA GTTCAACGAG 
GACGGCTCCT TCATTGGCCA GTACACAGTG AAAAAGGACA AGGAGGAAAC GGAAGGCAAC 
GAGAGCTCAG AAGCCACGTC CCCAGTCAAT GCTATCTACT CATTAGCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298912.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154398.1 Gallus gallus neurofascin (NFASC), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OGa46519
Clone ID Related Accession (Same CDS sequence)	XM_015298912.1 , XM_015298912.2
Accession Version	XM_015298912.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA 
ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC 
CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG 
TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG 
GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC 
AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG 
CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA 
GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT 
TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG 
ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC 
AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC 
ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC 
CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG 
GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC 
ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA 
GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT 
CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG 
GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA 
TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC 
AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC 
TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG 
AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC 
GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT 
GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG 
ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC 
AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC 
AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT 
TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG 
GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG 
GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA 
GATTTGCCCA GCTCCCCCAG GTACTTAAGA ATCCGACAGC CAAATCTGGA AAGCATCAAT 
TTGGAGTGGG ATCACCCAGA ACATCCCAAT GGAGTCCTCA CGGGATACAA CCTTAGATAT 
CAAGCCTTTA ACGGATCCAA AACGGGCAGA ACCCTGGTAG AGAACTTCTC TCCCAACCAG 
ACAAGGTTCA CCGTGCAGAG GACAGACCCC ATCTCGCGCT ATCGATTCTT CCTGCGTGCT 
CGGACACAGG TGGGAGATGG AGAAGTCATA GTGGAAGAGT CACCAGCGCT CCTGAATGAA 
GCCACGCCAA CCCCAGCCTC CACCTGGTTG CCTCCACCTA CAACCGAGCT AACTCCAGCA 
GCCACCATTG CCACCACAAC CACCACTGCC ACCCCTACTA CTGAAACCCC TCCTACTGAA 
ATCCCTACTA CTGCCATCCC TACCACCACC ACTACTACAA CCACCACAGC TGCCAGCACC 
GTCGCAAGCA CTACAACAAC TGCAGAGAGG GCTGCGGCAG CCACCACAAA ACAGGAGCTG 
GCCACCAATG GATCGTCCAT ATGGGACATA AGAGCTTGGG CTAATAGTAA CTGGGCAAAC 
ATCACCTGGA GCCACAATTA CTCTGCAGGA ACTGACTTTG TGGTTAAGTA TATCACCAGT 
AACAAGACAG AGAAATCTAT CCCCGTTAAA GCTCAGACCC CCTCCTCTGT GCAGCTGGCC 
AATCTGACGC CGGGGATGGT GTACAAGCTG TGGGTGTTCC CCATATGGTC TAGCCCCAGC 
GAGCACTCGT ACATAACCTT TACCACCAGC TCAGCTTACA CCAAGAACCA CGTGGACATC 
GCGACCCAGG GCTGGTTCAT CGGGCTGATG TGTGCCATCG CCCTCCTGGT CCTCATTCTG 
CTGATTGTTT GCTTCATTAA GAGGAGCAGA GGAGGGAAAT ACCCAGTGCG TGACAACAAA 
GATGAGCACC TGAATCCTGA AGACAAGAAC GTAGAAGATG GCTCATTCGA CTACAGGTCT 
CTTGAAAGCG ATGAAGACAA CAAACCACTG CCCAACAGTC AGACCTCCCT GGATGGCACG 
ATAAAGCAAC AGGAGAGTGA CGACAGCTTG GTGGACTACG GAGAGGGTGG GGAAGGGCAG 
TTCAACGAGG ACGGCTCCTT CATTGGCCAG TACACAGTGA AAAAGGACAA GGAGGAAACG 
GAAGGCAACG AGAGCTCAGA AGCCACGTCC CCAGTCAATG CTATCTACTC ATTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154398.1
CDS	159..3878
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTTRGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDLPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELATNGSSIWDIRAWANSNWANITWSHNYSAGTDFVVKYITSNKTEKSIPVKAQTPSSVQLANLTPGMVYKLWVFPIWSSPSEHSYITFTTSSAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298912.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298912.1

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA 
ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC 
CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG 
TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG 
GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC 
AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG 
CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA 
GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT 
TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG 
ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC 
AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC 
ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC 
CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG 
GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC 
ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA 
GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT 
CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG 
GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA 
TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC 
AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC 
TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG 
AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC 
GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT 
GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG 
ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC 
AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC 
AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT 
TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG 
GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG 
GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA 
GATTTGCCCA GCTCCCCCAG GTACTTAAGA ATCCGACAGC CAAATCTGGA AAGCATCAAT 
TTGGAGTGGG ATCACCCAGA ACATCCCAAT GGAGTCCTCA CGGGATACAA CCTTAGATAT 
CAAGCCTTTA ACGGATCCAA AACGGGCAGA ACCCTGGTAG AGAACTTCTC TCCCAACCAG 
ACAAGGTTCA CCGTGCAGAG GACAGACCCC ATCTCGCGCT ATCGATTCTT CCTGCGTGCT 
CGGACACAGG TGGGAGATGG AGAAGTCATA GTGGAAGAGT CACCAGCGCT CCTGAATGAA 
GCCACGCCAA CCCCAGCCTC CACCTGGTTG CCTCCACCTA CAACCGAGCT AACTCCAGCA 
GCCACCATTG CCACCACAAC CACCACTGCC ACCCCTACTA CTGAAACCCC TCCTACTGAA 
ATCCCTACTA CTGCCATCCC TACCACCACC ACTACTACAA CCACCACAGC TGCCAGCACC 
GTCGCAAGCA CTACAACAAC TGCAGAGAGG GCTGCGGCAG CCACCACAAA ACAGGAGCTG 
GCCACCAATG GATCGTCCAT ATGGGACATA AGAGCTTGGG CTAATAGTAA CTGGGCAAAC 
ATCACCTGGA GCCACAATTA CTCTGCAGGA ACTGACTTTG TGGTTAAGTA TATCACCAGT 
AACAAGACAG AGAAATCTAT CCCCGTTAAA GCTCAGACCC CCTCCTCTGT GCAGCTGGCC 
AATCTGACGC CGGGGATGGT GTACAAGCTG TGGGTGTTCC CCATATGGTC TAGCCCCAGC 
GAGCACTCGT ACATAACCTT TACCACCAGC TCAGCTTACA CCAAGAACCA CGTGGACATC 
GCGACCCAGG GCTGGTTCAT CGGGCTGATG TGTGCCATCG CCCTCCTGGT CCTCATTCTG 
CTGATTGTTT GCTTCATTAA GAGGAGCAGA GGAGGGAAAT ACCCAGTGCG TGACAACAAA 
GATGAGCACC TGAATCCTGA AGACAAGAAC GTAGAAGATG GCTCATTCGA CTACAGGTCT 
CTTGAAAGCG ATGAAGACAA CAAACCACTG CCCAACAGTC AGACCTCCCT GGATGGCACG 
ATAAAGCAAC AGGAGAGTGA CGACAGCTTG GTGGACTACG GAGAGGGTGG GGAAGGGCAG 
TTCAACGAGG ACGGCTCCTT CATTGGCCAG TACACAGTGA AAAAGGACAA GGAGGAAACG 
GAAGGCAACG AGAGCTCAGA AGCCACGTCC CCAGTCAATG CTATCTACTC ATTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298912.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154398.1 Gallus gallus neurofascin (NFASC), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OGa46519
Clone ID Related Accession (Same CDS sequence)	XM_015298912.1 , XM_015298912.2
Accession Version	XM_015298912.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298912.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA 
ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC 
CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG 
TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG 
GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC 
AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG 
CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA 
GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT 
TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG 
ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC 
AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC 
ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC 
CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG 
GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC 
ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA 
GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT 
CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG 
GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA 
TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC 
AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC 
TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG 
AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC 
GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT 
GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG 
ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC 
AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC 
AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT 
TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG 
GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG 
GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA 
GATTTGCCCA GCTCCCCCAG GTACTTAAGA ATCCGACAGC CAAATCTGGA AAGCATCAAT 
TTGGAGTGGG ATCACCCAGA ACATCCCAAT GGAGTCCTCA CGGGATACAA CCTTAGATAT 
CAAGCCTTTA ACGGATCCAA AACGGGCAGA ACCCTGGTAG AGAACTTCTC TCCCAACCAG 
ACAAGGTTCA CCGTGCAGAG GACAGACCCC ATCTCGCGCT ATCGATTCTT CCTGCGTGCT 
CGGACACAGG TGGGAGATGG AGAAGTCATA GTGGAAGAGT CACCAGCGCT CCTGAATGAA 
GCCACGCCAA CCCCAGCCTC CACCTGGTTG CCTCCACCTA CAACCGAGCT AACTCCAGCA 
GCCACCATTG CCACCACAAC CACCACTGCC ACCCCTACTA CTGAAACCCC TCCTACTGAA 
ATCCCTACTA CTGCCATCCC TACCACCACC ACTACTACAA CCACCACAGC TGCCAGCACC 
GTCGCAAGCA CTACAACAAC TGCAGAGAGG GCTGCGGCAG CCACCACAAA ACAGGAGCTG 
GCCACCAATG GATCGTCCAT ATGGGACATA AGAGCTTGGG CTAATAGTAA CTGGGCAAAC 
ATCACCTGGA GCCACAATTA CTCTGCAGGA ACTGACTTTG TGGTTAAGTA TATCACCAGT 
AACAAGACAG AGAAATCTAT CCCCGTTAAA GCTCAGACCC CCTCCTCTGT GCAGCTGGCC 
AATCTGACGC CGGGGATGGT GTACAAGCTG TGGGTGTTCC CCATATGGTC TAGCCCCAGC 
GAGCACTCGT ACATAACCTT TACCACCAGC TCAGCTTACA CCAAGAACCA CGTGGACATC 
GCGACCCAGG GCTGGTTCAT CGGGCTGATG TGTGCCATCG CCCTCCTGGT CCTCATTCTG 
CTGATTGTTT GCTTCATTAA GAGGAGCAGA GGAGGGAAAT ACCCAGTGCG TGACAACAAA 
GATGAGCACC TGAATCCTGA AGACAAGAAC GTAGAAGATG GCTCATTCGA CTACAGGTCT 
CTTGAAAGCG ATGAAGACAA CAAACCACTG CCCAACAGTC AGACCTCCCT GGATGGCACG 
ATAAAGCAAC AGGAGAGTGA CGACAGCTTG GTGGACTACG GAGAGGGTGG GGAAGGGCAG 
TTCAACGAGG ACGGCTCCTT CATTGGCCAG TACACAGTGA AAAAGGACAA GGAGGAAACG 
GAAGGCAACG AGAGCTCAGA AGCCACGTCC CCAGTCAATG CTATCTACTC ATTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154398.1
CDS	169..3888
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTTRGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDLPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELATNGSSIWDIRAWANSNWANITWSHNYSAGTDFVVKYITSNKTEKSIPVKAQTPSSVQLANLTPGMVYKLWVFPIWSSPSEHSYITFTTSSAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298912.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298912.2

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA 
ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC 
CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG 
TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG 
GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC 
AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG 
CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA 
GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT 
TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG 
ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC 
AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC 
ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC 
CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG 
GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC 
ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA 
GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT 
CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG 
GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA 
TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC 
AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC 
TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG 
AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC 
GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT 
GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG 
ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC 
AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC 
AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT 
TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG 
GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG 
GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA 
GATTTGCCCA GCTCCCCCAG GTACTTAAGA ATCCGACAGC CAAATCTGGA AAGCATCAAT 
TTGGAGTGGG ATCACCCAGA ACATCCCAAT GGAGTCCTCA CGGGATACAA CCTTAGATAT 
CAAGCCTTTA ACGGATCCAA AACGGGCAGA ACCCTGGTAG AGAACTTCTC TCCCAACCAG 
ACAAGGTTCA CCGTGCAGAG GACAGACCCC ATCTCGCGCT ATCGATTCTT CCTGCGTGCT 
CGGACACAGG TGGGAGATGG AGAAGTCATA GTGGAAGAGT CACCAGCGCT CCTGAATGAA 
GCCACGCCAA CCCCAGCCTC CACCTGGTTG CCTCCACCTA CAACCGAGCT AACTCCAGCA 
GCCACCATTG CCACCACAAC CACCACTGCC ACCCCTACTA CTGAAACCCC TCCTACTGAA 
ATCCCTACTA CTGCCATCCC TACCACCACC ACTACTACAA CCACCACAGC TGCCAGCACC 
GTCGCAAGCA CTACAACAAC TGCAGAGAGG GCTGCGGCAG CCACCACAAA ACAGGAGCTG 
GCCACCAATG GATCGTCCAT ATGGGACATA AGAGCTTGGG CTAATAGTAA CTGGGCAAAC 
ATCACCTGGA GCCACAATTA CTCTGCAGGA ACTGACTTTG TGGTTAAGTA TATCACCAGT 
AACAAGACAG AGAAATCTAT CCCCGTTAAA GCTCAGACCC CCTCCTCTGT GCAGCTGGCC 
AATCTGACGC CGGGGATGGT GTACAAGCTG TGGGTGTTCC CCATATGGTC TAGCCCCAGC 
GAGCACTCGT ACATAACCTT TACCACCAGC TCAGCTTACA CCAAGAACCA CGTGGACATC 
GCGACCCAGG GCTGGTTCAT CGGGCTGATG TGTGCCATCG CCCTCCTGGT CCTCATTCTG 
CTGATTGTTT GCTTCATTAA GAGGAGCAGA GGAGGGAAAT ACCCAGTGCG TGACAACAAA 
GATGAGCACC TGAATCCTGA AGACAAGAAC GTAGAAGATG GCTCATTCGA CTACAGGTCT 
CTTGAAAGCG ATGAAGACAA CAAACCACTG CCCAACAGTC AGACCTCCCT GGATGGCACG 
ATAAAGCAAC AGGAGAGTGA CGACAGCTTG GTGGACTACG GAGAGGGTGG GGAAGGGCAG 
TTCAACGAGG ACGGCTCCTT CATTGGCCAG TACACAGTGA AAAAGGACAA GGAGGAAACG 
GAAGGCAACG AGAGCTCAGA AGCCACGTCC CCAGTCAATG CTATCTACTC ATTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298913.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154399.1 Gallus gallus neurofascin (NFASC), transcript variant X12, mRNA.

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CloneID	OGa46520
Clone ID Related Accession (Same CDS sequence)	XM_015298913.2 , XM_015298913.1
Accession Version	XM_015298913.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298913.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCCACGCC AACCCCAGCT TACACCAAGA ACCACGTGGA CATCGCGACC 
CAGGGCTGGT TCATCGGGCT GATGTGTGCC ATCGCCCTCC TGGTCCTCAT TCTGCTGATT 
GTTTGCTTCA TTAAGAGGAG CAGAGGAGGG AAATACCCAG TGCGTGACAA CAAAGATGAG 
CACCTGAATC CTGAAGACAA GAACGTAGAA GATGGCTCAT TCGACTACAG GTCTCTTGAA 
AGCGATGAAG ACAACAAACC ACTGCCCAAC AGTCAGACCT CCCTGGATGG CACGATAAAG 
CAACAGGAGA GTGACGACAG CTTGGTGGAC TACGGAGAGG GTGGGGAAGG GCAGTTCAAC 
GAGGACGGCT CCTTCATTGG CCAGTACACA GTGAAAAAGG ACAAGGAGGA AACGGAAGGC 
AACGAGAGCT CAGAAGCCAC GTCCCCAGTC AATGCTATCT ACTCATTAGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154399.1
CDS	169..3762
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298913.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298913.2

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCCACGCC AACCCCAGCT TACACCAAGA ACCACGTGGA CATCGCGACC 
CAGGGCTGGT TCATCGGGCT GATGTGTGCC ATCGCCCTCC TGGTCCTCAT TCTGCTGATT 
GTTTGCTTCA TTAAGAGGAG CAGAGGAGGG AAATACCCAG TGCGTGACAA CAAAGATGAG 
CACCTGAATC CTGAAGACAA GAACGTAGAA GATGGCTCAT TCGACTACAG GTCTCTTGAA 
AGCGATGAAG ACAACAAACC ACTGCCCAAC AGTCAGACCT CCCTGGATGG CACGATAAAG 
CAACAGGAGA GTGACGACAG CTTGGTGGAC TACGGAGAGG GTGGGGAAGG GCAGTTCAAC 
GAGGACGGCT CCTTCATTGG CCAGTACACA GTGAAAAAGG ACAAGGAGGA AACGGAAGGC 
AACGAGAGCT CAGAAGCCAC GTCCCCAGTC AATGCTATCT ACTCATTAGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298913.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154399.1 Gallus gallus neurofascin (NFASC), transcript variant X11, mRNA.

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CloneID	OGa46520
Clone ID Related Accession (Same CDS sequence)	XM_015298913.2 , XM_015298913.1
Accession Version	XM_015298913.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCCACGCC AACCCCAGCT TACACCAAGA ACCACGTGGA CATCGCGACC 
CAGGGCTGGT TCATCGGGCT GATGTGTGCC ATCGCCCTCC TGGTCCTCAT TCTGCTGATT 
GTTTGCTTCA TTAAGAGGAG CAGAGGAGGG AAATACCCAG TGCGTGACAA CAAAGATGAG 
CACCTGAATC CTGAAGACAA GAACGTAGAA GATGGCTCAT TCGACTACAG GTCTCTTGAA 
AGCGATGAAG ACAACAAACC ACTGCCCAAC AGTCAGACCT CCCTGGATGG CACGATAAAG 
CAACAGGAGA GTGACGACAG CTTGGTGGAC TACGGAGAGG GTGGGGAAGG GCAGTTCAAC 
GAGGACGGCT CCTTCATTGG CCAGTACACA GTGAAAAAGG ACAAGGAGGA AACGGAAGGC 
AACGAGAGCT CAGAAGCCAC GTCCCCAGTC AATGCTATCT ACTCATTAGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154399.1
CDS	159..3752
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298913.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298913.1

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCCACGCC AACCCCAGCT TACACCAAGA ACCACGTGGA CATCGCGACC 
CAGGGCTGGT TCATCGGGCT GATGTGTGCC ATCGCCCTCC TGGTCCTCAT TCTGCTGATT 
GTTTGCTTCA TTAAGAGGAG CAGAGGAGGG AAATACCCAG TGCGTGACAA CAAAGATGAG 
CACCTGAATC CTGAAGACAA GAACGTAGAA GATGGCTCAT TCGACTACAG GTCTCTTGAA 
AGCGATGAAG ACAACAAACC ACTGCCCAAC AGTCAGACCT CCCTGGATGG CACGATAAAG 
CAACAGGAGA GTGACGACAG CTTGGTGGAC TACGGAGAGG GTGGGGAAGG GCAGTTCAAC 
GAGGACGGCT CCTTCATTGG CCAGTACACA GTGAAAAAGG ACAAGGAGGA AACGGAAGGC 
AACGAGAGCT CAGAAGCCAC GTCCCCAGTC AATGCTATCT ACTCATTAGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298914.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154400.1 Gallus gallus neurofascin (NFASC), transcript variant X12, mRNA.

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CloneID	OGa46521
Clone ID Related Accession (Same CDS sequence)	XM_015298914.1 , XM_015298914.2
Accession Version	XM_015298914.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCTTACAC CAAGAACCAC GTGGACATCG CGACCCAGGG CTGGTTCATC 
GGGCTGATGT GTGCCATCGC CCTCCTGGTC CTCATTCTGC TGATTGTTTG CTTCATTAAG 
AGGAGCAGAG GAGGGAAATA CCCAGTGCGT GACAACAAAG ATGAGCACCT GAATCCTGAA 
GACAAGAACG TAGAAGATGG CTCATTCGAC TACAGGTCTC TTGAAAGCGA TGAAGACAAC 
AAACCACTGC CCAACAGTCA GACCTCCCTG GATGGCACGA TAAAGCAACA GGAGAGTGAC 
GACAGCTTGG TGGACTACGG AGAGGGTGGG GAAGGGCAGT TCAACGAGGA CGGCTCCTTC 
ATTGGCCAGT ACACAGTGAA AAAGGACAAG GAGGAAACGG AAGGCAACGA GAGCTCAGAA 
GCCACGTCCC CAGTCAATGC TATCTACTCA TTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154400.1
CDS	159..3737
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298914.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298914.1

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCTTACAC CAAGAACCAC GTGGACATCG CGACCCAGGG CTGGTTCATC 
GGGCTGATGT GTGCCATCGC CCTCCTGGTC CTCATTCTGC TGATTGTTTG CTTCATTAAG 
AGGAGCAGAG GAGGGAAATA CCCAGTGCGT GACAACAAAG ATGAGCACCT GAATCCTGAA 
GACAAGAACG TAGAAGATGG CTCATTCGAC TACAGGTCTC TTGAAAGCGA TGAAGACAAC 
AAACCACTGC CCAACAGTCA GACCTCCCTG GATGGCACGA TAAAGCAACA GGAGAGTGAC 
GACAGCTTGG TGGACTACGG AGAGGGTGGG GAAGGGCAGT TCAACGAGGA CGGCTCCTTC 
ATTGGCCAGT ACACAGTGAA AAAGGACAAG GAGGAAACGG AAGGCAACGA GAGCTCAGAA 
GCCACGTCCC CAGTCAATGC TATCTACTCA TTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298914.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154400.1 Gallus gallus neurofascin (NFASC), transcript variant X13, mRNA.

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CloneID	OGa46521
Clone ID Related Accession (Same CDS sequence)	XM_015298914.1 , XM_015298914.2
Accession Version	XM_015298914.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298914.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCTTACAC CAAGAACCAC GTGGACATCG CGACCCAGGG CTGGTTCATC 
GGGCTGATGT GTGCCATCGC CCTCCTGGTC CTCATTCTGC TGATTGTTTG CTTCATTAAG 
AGGAGCAGAG GAGGGAAATA CCCAGTGCGT GACAACAAAG ATGAGCACCT GAATCCTGAA 
GACAAGAACG TAGAAGATGG CTCATTCGAC TACAGGTCTC TTGAAAGCGA TGAAGACAAC 
AAACCACTGC CCAACAGTCA GACCTCCCTG GATGGCACGA TAAAGCAACA GGAGAGTGAC 
GACAGCTTGG TGGACTACGG AGAGGGTGGG GAAGGGCAGT TCAACGAGGA CGGCTCCTTC 
ATTGGCCAGT ACACAGTGAA AAAGGACAAG GAGGAAACGG AAGGCAACGA GAGCTCAGAA 
GCCACGTCCC CAGTCAATGC TATCTACTCA TTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154400.1
CDS	169..3747
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298914.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298914.2

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCTTACAC CAAGAACCAC GTGGACATCG CGACCCAGGG CTGGTTCATC 
GGGCTGATGT GTGCCATCGC CCTCCTGGTC CTCATTCTGC TGATTGTTTG CTTCATTAAG 
AGGAGCAGAG GAGGGAAATA CCCAGTGCGT GACAACAAAG ATGAGCACCT GAATCCTGAA 
GACAAGAACG TAGAAGATGG CTCATTCGAC TACAGGTCTC TTGAAAGCGA TGAAGACAAC 
AAACCACTGC CCAACAGTCA GACCTCCCTG GATGGCACGA TAAAGCAACA GGAGAGTGAC 
GACAGCTTGG TGGACTACGG AGAGGGTGGG GAAGGGCAGT TCAACGAGGA CGGCTCCTTC 
ATTGGCCAGT ACACAGTGAA AAAGGACAAG GAGGAAACGG AAGGCAACGA GAGCTCAGAA 
GCCACGTCCC CAGTCAATGC TATCTACTCA TTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298915.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154401.1 Gallus gallus neurofascin (NFASC), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OGa46522
Clone ID Related Accession (Same CDS sequence)	XM_015298915.1 , XM_015298915.2
Accession Version	XM_015298915.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG 
CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC 
AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA 
AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG 
GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC 
ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG 
AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT 
CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG 
GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG 
GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG 
AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA 
AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC 
CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC 
ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA 
GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG 
GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG 
ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG 
GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC 
CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG 
GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC 
AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG 
CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC 
CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC 
CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG 
AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC 
AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC 
GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC 
CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC 
AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT 
GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC 
GTGCTAGCAA TCCCTGCTAA CCGTTTGAGA GACTTACCTA AAGAGCGACC CGACCGGCCC 
CGGGACCTGG AGCTGTCAGA CCTGGCTGAG AGGAGCGTGA AGCTGACATG GATTCCTGGC 
GATGACAACA ACAGCCCCAT CACAGACTAC ATCGTCCAGT TTGAGGAGGA CCGCTTCCAG 
CCTGGCACGT GGCACAACCA CTCCAGGTAT CCTGGGAATG TCAACTCGGC CCTCCTGAGC 
CTCTCTCCTT ACGTCAACTA CCAATTTCGG GTGATTGCAG TGAACGACGT GGGCAGCAGC 
CTGCCCAGCA TGCCCTCAGA ACGATACCAG ACCAGCGGGG CACGACCTGA AATTAACCCA 
ACAGGAGTTC AAGGAGCAGG GACCCAAAAA AACAACATGG AGATAACCTG GACGCCTCTG 
AATGCAACTC AAGCCTATGG GCCCAACCTC CGTTACATCG TGCGGTGGAG GCGAAGGGAC 
CCACGTGGGA GTTGGTACAA CGAGACGGTG AAGGCACCAC GACACGTCGT CTGGAACACA 
CCCATCTACG TCCCCTACGA GATCAAAGTG CAGGCAGAGA ATGACTTTGG TAGAGCTCCA 
GAGCCTGAGA CCTACATCGG CTACTCAGGG GAAGATTATC CCAAGGCTGC ACCTACAGAT 
GTTAGGATAA GAGTTTTAAA CAGCACTGCC ATTGCTCTGA CATGGACCCG CGTGCACCTG 
GACACCATCC AGGGACAGCT TAAGGAGTAC AGAGCCTATT TCTGGAGAGA CAGTAGTTTG 
CTGAAGAACC TGTGGGTCTC CAAAAAACGG CAGTATGTGA GTTTTCCTGG AGACCGAAAC 
CGGGGCATAG TGTCCCGGCT GTTCCCTTAC AGCAACTACA AGCTTGAGAT GGTTGTAACC 
AACGGGAGAG GCGATGGGCC CCGCAGCGAA GTCAAGGAGT TCCCCACACC TGAAGGAGTG 
CCCAGCTCCC CCAGGTACTT AAGAATCCGA CAGCCAAATC TGGAAAGCAT CAATTTGGAG 
TGGGATCACC CAGAACATCC CAATGGAGTC CTCACGGGAT ACAACCTTAG ATATCAAGCC 
TTTAACGGAT CCAAAACGGG CAGAACCCTG GTAGAGAACT TCTCTCCCAA CCAGACAAGG 
TTCACCGTGC AGAGGACAGA CCCCATCTCG CGCTATCGAT TCTTCCTGCG TGCTCGGACA 
CAGGTGGGAG ATGGAGAAGT CATAGTGGAA GAGTCACCAG CGCTCCTGAA TGAAGCCACG 
CCAACCCCAG CTTACACCAA GAACCACGTG GACATCGCGA CCCAGGGCTG GTTCATCGGG 
CTGATGTGTG CCATCGCCCT CCTGGTCCTC ATTCTGCTGA TTGTTTGCTT CATTAAGAGG 
AGCAGAGGAG GGAAATACCC AGTGCGTGAC AACAAAGATG AGCACCTGAA TCCTGAAGAC 
AAGAACGTAG AAGATGGCTC ATTCGACTAC AGCGATGAAG ACAACAAACC ACTGCCCAAC 
AGTCAGACCT CCCTGGATGG CACGATAAAG CAACAGGAGA GTGACGACAG CTTGGTGGAC 
TACGGAGAGG GTGGGGAAGG GCAGTTCAAC GAGGACGGCT CCTTCATTGG CCAGTACACA 
GTGAAAAAGG ACAAGGAGGA AACGGAAGGC AACGAGAGCT CAGAAGCCAC GTCCCCAGTC 
AATGCTATCT ACTCATTAGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154401.1
CDS	159..3722
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDLPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYSDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298915.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298915.1

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG 
CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC 
AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA 
AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG 
GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC 
ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG 
AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT 
CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG 
GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG 
GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG 
AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA 
AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC 
CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC 
ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA 
GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG 
GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG 
ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG 
GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC 
CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG 
GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC 
AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG 
CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC 
CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC 
CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG 
AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC 
AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC 
GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC 
CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC 
AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT 
GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC 
GTGCTAGCAA TCCCTGCTAA CCGTTTGAGA GACTTACCTA AAGAGCGACC CGACCGGCCC 
CGGGACCTGG AGCTGTCAGA CCTGGCTGAG AGGAGCGTGA AGCTGACATG GATTCCTGGC 
GATGACAACA ACAGCCCCAT CACAGACTAC ATCGTCCAGT TTGAGGAGGA CCGCTTCCAG 
CCTGGCACGT GGCACAACCA CTCCAGGTAT CCTGGGAATG TCAACTCGGC CCTCCTGAGC 
CTCTCTCCTT ACGTCAACTA CCAATTTCGG GTGATTGCAG TGAACGACGT GGGCAGCAGC 
CTGCCCAGCA TGCCCTCAGA ACGATACCAG ACCAGCGGGG CACGACCTGA AATTAACCCA 
ACAGGAGTTC AAGGAGCAGG GACCCAAAAA AACAACATGG AGATAACCTG GACGCCTCTG 
AATGCAACTC AAGCCTATGG GCCCAACCTC CGTTACATCG TGCGGTGGAG GCGAAGGGAC 
CCACGTGGGA GTTGGTACAA CGAGACGGTG AAGGCACCAC GACACGTCGT CTGGAACACA 
CCCATCTACG TCCCCTACGA GATCAAAGTG CAGGCAGAGA ATGACTTTGG TAGAGCTCCA 
GAGCCTGAGA CCTACATCGG CTACTCAGGG GAAGATTATC CCAAGGCTGC ACCTACAGAT 
GTTAGGATAA GAGTTTTAAA CAGCACTGCC ATTGCTCTGA CATGGACCCG CGTGCACCTG 
GACACCATCC AGGGACAGCT TAAGGAGTAC AGAGCCTATT TCTGGAGAGA CAGTAGTTTG 
CTGAAGAACC TGTGGGTCTC CAAAAAACGG CAGTATGTGA GTTTTCCTGG AGACCGAAAC 
CGGGGCATAG TGTCCCGGCT GTTCCCTTAC AGCAACTACA AGCTTGAGAT GGTTGTAACC 
AACGGGAGAG GCGATGGGCC CCGCAGCGAA GTCAAGGAGT TCCCCACACC TGAAGGAGTG 
CCCAGCTCCC CCAGGTACTT AAGAATCCGA CAGCCAAATC TGGAAAGCAT CAATTTGGAG 
TGGGATCACC CAGAACATCC CAATGGAGTC CTCACGGGAT ACAACCTTAG ATATCAAGCC 
TTTAACGGAT CCAAAACGGG CAGAACCCTG GTAGAGAACT TCTCTCCCAA CCAGACAAGG 
TTCACCGTGC AGAGGACAGA CCCCATCTCG CGCTATCGAT TCTTCCTGCG TGCTCGGACA 
CAGGTGGGAG ATGGAGAAGT CATAGTGGAA GAGTCACCAG CGCTCCTGAA TGAAGCCACG 
CCAACCCCAG CTTACACCAA GAACCACGTG GACATCGCGA CCCAGGGCTG GTTCATCGGG 
CTGATGTGTG CCATCGCCCT CCTGGTCCTC ATTCTGCTGA TTGTTTGCTT CATTAAGAGG 
AGCAGAGGAG GGAAATACCC AGTGCGTGAC AACAAAGATG AGCACCTGAA TCCTGAAGAC 
AAGAACGTAG AAGATGGCTC ATTCGACTAC AGCGATGAAG ACAACAAACC ACTGCCCAAC 
AGTCAGACCT CCCTGGATGG CACGATAAAG CAACAGGAGA GTGACGACAG CTTGGTGGAC 
TACGGAGAGG GTGGGGAAGG GCAGTTCAAC GAGGACGGCT CCTTCATTGG CCAGTACACA 
GTGAAAAAGG ACAAGGAGGA AACGGAAGGC AACGAGAGCT CAGAAGCCAC GTCCCCAGTC 
AATGCTATCT ACTCATTAGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298915.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154401.1 Gallus gallus neurofascin (NFASC), transcript variant X15, mRNA.

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CloneID	OGa46522
Clone ID Related Accession (Same CDS sequence)	XM_015298915.1 , XM_015298915.2
Accession Version	XM_015298915.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298915.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG 
CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC 
AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA 
AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG 
GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC 
ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG 
AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT 
CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG 
GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG 
GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG 
AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA 
AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC 
CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC 
ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA 
GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG 
GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG 
ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG 
GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC 
CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG 
GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC 
AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG 
CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC 
CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC 
CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG 
AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC 
AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC 
GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC 
CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC 
AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT 
GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC 
GTGCTAGCAA TCCCTGCTAA CCGTTTGAGA GACTTACCTA AAGAGCGACC CGACCGGCCC 
CGGGACCTGG AGCTGTCAGA CCTGGCTGAG AGGAGCGTGA AGCTGACATG GATTCCTGGC 
GATGACAACA ACAGCCCCAT CACAGACTAC ATCGTCCAGT TTGAGGAGGA CCGCTTCCAG 
CCTGGCACGT GGCACAACCA CTCCAGGTAT CCTGGGAATG TCAACTCGGC CCTCCTGAGC 
CTCTCTCCTT ACGTCAACTA CCAATTTCGG GTGATTGCAG TGAACGACGT GGGCAGCAGC 
CTGCCCAGCA TGCCCTCAGA ACGATACCAG ACCAGCGGGG CACGACCTGA AATTAACCCA 
ACAGGAGTTC AAGGAGCAGG GACCCAAAAA AACAACATGG AGATAACCTG GACGCCTCTG 
AATGCAACTC AAGCCTATGG GCCCAACCTC CGTTACATCG TGCGGTGGAG GCGAAGGGAC 
CCACGTGGGA GTTGGTACAA CGAGACGGTG AAGGCACCAC GACACGTCGT CTGGAACACA 
CCCATCTACG TCCCCTACGA GATCAAAGTG CAGGCAGAGA ATGACTTTGG TAGAGCTCCA 
GAGCCTGAGA CCTACATCGG CTACTCAGGG GAAGATTATC CCAAGGCTGC ACCTACAGAT 
GTTAGGATAA GAGTTTTAAA CAGCACTGCC ATTGCTCTGA CATGGACCCG CGTGCACCTG 
GACACCATCC AGGGACAGCT TAAGGAGTAC AGAGCCTATT TCTGGAGAGA CAGTAGTTTG 
CTGAAGAACC TGTGGGTCTC CAAAAAACGG CAGTATGTGA GTTTTCCTGG AGACCGAAAC 
CGGGGCATAG TGTCCCGGCT GTTCCCTTAC AGCAACTACA AGCTTGAGAT GGTTGTAACC 
AACGGGAGAG GCGATGGGCC CCGCAGCGAA GTCAAGGAGT TCCCCACACC TGAAGGAGTG 
CCCAGCTCCC CCAGGTACTT AAGAATCCGA CAGCCAAATC TGGAAAGCAT CAATTTGGAG 
TGGGATCACC CAGAACATCC CAATGGAGTC CTCACGGGAT ACAACCTTAG ATATCAAGCC 
TTTAACGGAT CCAAAACGGG CAGAACCCTG GTAGAGAACT TCTCTCCCAA CCAGACAAGG 
TTCACCGTGC AGAGGACAGA CCCCATCTCG CGCTATCGAT TCTTCCTGCG TGCTCGGACA 
CAGGTGGGAG ATGGAGAAGT CATAGTGGAA GAGTCACCAG CGCTCCTGAA TGAAGCCACG 
CCAACCCCAG CTTACACCAA GAACCACGTG GACATCGCGA CCCAGGGCTG GTTCATCGGG 
CTGATGTGTG CCATCGCCCT CCTGGTCCTC ATTCTGCTGA TTGTTTGCTT CATTAAGAGG 
AGCAGAGGAG GGAAATACCC AGTGCGTGAC AACAAAGATG AGCACCTGAA TCCTGAAGAC 
AAGAACGTAG AAGATGGCTC ATTCGACTAC AGCGATGAAG ACAACAAACC ACTGCCCAAC 
AGTCAGACCT CCCTGGATGG CACGATAAAG CAACAGGAGA GTGACGACAG CTTGGTGGAC 
TACGGAGAGG GTGGGGAAGG GCAGTTCAAC GAGGACGGCT CCTTCATTGG CCAGTACACA 
GTGAAAAAGG ACAAGGAGGA AACGGAAGGC AACGAGAGCT CAGAAGCCAC GTCCCCAGTC 
AATGCTATCT ACTCATTAGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154401.1
CDS	169..3732
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDLPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYSDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298915.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298915.2

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG 
CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC 
AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA 
AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG 
GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC 
ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG 
AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT 
CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG 
GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG 
GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG 
AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA 
AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC 
CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC 
ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA 
GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG 
GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG 
ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG 
GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC 
CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG 
GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC 
AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG 
CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC 
CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC 
CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG 
AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC 
AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC 
GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC 
CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC 
AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT 
GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC 
GTGCTAGCAA TCCCTGCTAA CCGTTTGAGA GACTTACCTA AAGAGCGACC CGACCGGCCC 
CGGGACCTGG AGCTGTCAGA CCTGGCTGAG AGGAGCGTGA AGCTGACATG GATTCCTGGC 
GATGACAACA ACAGCCCCAT CACAGACTAC ATCGTCCAGT TTGAGGAGGA CCGCTTCCAG 
CCTGGCACGT GGCACAACCA CTCCAGGTAT CCTGGGAATG TCAACTCGGC CCTCCTGAGC 
CTCTCTCCTT ACGTCAACTA CCAATTTCGG GTGATTGCAG TGAACGACGT GGGCAGCAGC 
CTGCCCAGCA TGCCCTCAGA ACGATACCAG ACCAGCGGGG CACGACCTGA AATTAACCCA 
ACAGGAGTTC AAGGAGCAGG GACCCAAAAA AACAACATGG AGATAACCTG GACGCCTCTG 
AATGCAACTC AAGCCTATGG GCCCAACCTC CGTTACATCG TGCGGTGGAG GCGAAGGGAC 
CCACGTGGGA GTTGGTACAA CGAGACGGTG AAGGCACCAC GACACGTCGT CTGGAACACA 
CCCATCTACG TCCCCTACGA GATCAAAGTG CAGGCAGAGA ATGACTTTGG TAGAGCTCCA 
GAGCCTGAGA CCTACATCGG CTACTCAGGG GAAGATTATC CCAAGGCTGC ACCTACAGAT 
GTTAGGATAA GAGTTTTAAA CAGCACTGCC ATTGCTCTGA CATGGACCCG CGTGCACCTG 
GACACCATCC AGGGACAGCT TAAGGAGTAC AGAGCCTATT TCTGGAGAGA CAGTAGTTTG 
CTGAAGAACC TGTGGGTCTC CAAAAAACGG CAGTATGTGA GTTTTCCTGG AGACCGAAAC 
CGGGGCATAG TGTCCCGGCT GTTCCCTTAC AGCAACTACA AGCTTGAGAT GGTTGTAACC 
AACGGGAGAG GCGATGGGCC CCGCAGCGAA GTCAAGGAGT TCCCCACACC TGAAGGAGTG 
CCCAGCTCCC CCAGGTACTT AAGAATCCGA CAGCCAAATC TGGAAAGCAT CAATTTGGAG 
TGGGATCACC CAGAACATCC CAATGGAGTC CTCACGGGAT ACAACCTTAG ATATCAAGCC 
TTTAACGGAT CCAAAACGGG CAGAACCCTG GTAGAGAACT TCTCTCCCAA CCAGACAAGG 
TTCACCGTGC AGAGGACAGA CCCCATCTCG CGCTATCGAT TCTTCCTGCG TGCTCGGACA 
CAGGTGGGAG ATGGAGAAGT CATAGTGGAA GAGTCACCAG CGCTCCTGAA TGAAGCCACG 
CCAACCCCAG CTTACACCAA GAACCACGTG GACATCGCGA CCCAGGGCTG GTTCATCGGG 
CTGATGTGTG CCATCGCCCT CCTGGTCCTC ATTCTGCTGA TTGTTTGCTT CATTAAGAGG 
AGCAGAGGAG GGAAATACCC AGTGCGTGAC AACAAAGATG AGCACCTGAA TCCTGAAGAC 
AAGAACGTAG AAGATGGCTC ATTCGACTAC AGCGATGAAG ACAACAAACC ACTGCCCAAC 
AGTCAGACCT CCCTGGATGG CACGATAAAG CAACAGGAGA GTGACGACAG CTTGGTGGAC 
TACGGAGAGG GTGGGGAAGG GCAGTTCAAC GAGGACGGCT CCTTCATTGG CCAGTACACA 
GTGAAAAAGG ACAAGGAGGA AACGGAAGGC AACGAGAGCT CAGAAGCCAC GTCCCCAGTC 
AATGCTATCT ACTCATTAGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298916.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154402.1 Gallus gallus neurofascin (NFASC), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OGa46523
Clone ID Related Accession (Same CDS sequence)	XM_015298916.1 , XM_015298916.2
Accession Version	XM_015298916.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG 
CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC 
AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA 
AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG 
GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC 
ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG 
AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT 
CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG 
GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG 
GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG 
AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA 
AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC 
CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC 
ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA 
GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG 
GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG 
ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG 
GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC 
CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG 
GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC 
AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG 
CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC 
CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC 
CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG 
AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC 
AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC 
GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC 
CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC 
AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT 
GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC 
GTGCTAGAGC GACCCGACCG GCCCCGGGAC CTGGAGCTGT CAGACCTGGC TGAGAGGAGC 
GTGAAGCTGA CATGGATTCC TGGCGATGAC AACAACAGCC CCATCACAGA CTACATCGTC 
CAGTTTGAGG AGGACCGCTT CCAGCCTGGC ACGTGGCACA ACCACTCCAG GTATCCTGGG 
AATGTCAACT CGGCCCTCCT GAGCCTCTCT CCTTACGTCA ACTACCAATT TCGGGTGATT 
GCAGTGAACG ACGTGGGCAG CAGCCTGCCC AGCATGCCCT CAGAACGATA CCAGACCAGC 
GGGGCACGAC CTGAAATTAA CCCAACAGGA GTTCAAGGAG CAGGGACCCA AAAAAACAAC 
ATGGAGATAA CCTGGACGCC TCTGAATGCA ACTCAAGCCT ATGGGCCCAA CCTCCGTTAC 
ATCGTGCGGT GGAGGCGAAG GGACCCACGT GGGAGTTGGT ACAACGAGAC GGTGAAGGCA 
CCACGACACG TCGTCTGGAA CACACCCATC TACGTCCCCT ACGAGATCAA AGTGCAGGCA 
GAGAATGACT TTGGTAGAGC TCCAGAGCCT GAGACCTACA TCGGCTACTC AGGGGAAGAT 
TATCCCAAGG CTGCACCTAC AGATGTTAGG ATAAGAGTTT TAAACAGCAC TGCCATTGCT 
CTGACATGGA CCCGCGTGCA CCTGGACACC ATCCAGGGAC AGCTTAAGGA GTACAGAGCC 
TATTTCTGGA GAGACAGTAG TTTGCTGAAG AACCTGTGGG TCTCCAAAAA ACGGCAGTAT 
GTGAGTTTTC CTGGAGACCG AAACCGGGGC ATAGTGTCCC GGCTGTTCCC TTACAGCAAC 
TACAAGCTTG AGATGGTTGT AACCAACGGG AGAGGCGATG GGCCCCGCAG CGAAGTCAAG 
GAGTTCCCCA CACCTGAAGG AGTGCCCAGC TCCCCCAGGT ACTTAAGAAT CCGACAGCCA 
AATCTGGAAA GCATCAATTT GGAGTGGGAT CACCCAGAAC ATCCCAATGG AGTCCTCACG 
GGATACAACC TTAGATATCA AGCCTTTAAC GGATCCAAAA CGGGCAGAAC CCTGGTAGAG 
AACTTCTCTC CCAACCAGAC AAGGTTCACC GTGCAGAGGA CAGACCCCAT CTCGCGCTAT 
CGATTCTTCC TGCGTGCTCG GACACAGGTG GGAGATGGAG AAGTCATAGT GGAAGAGTCA 
CCAGCGCTCC TGAATGAAGC CACGCCAACC CCAGCTTACA CCAAGAACCA CGTGGACATC 
GCGACCCAGG GCTGGTTCAT CGGGCTGATG TGTGCCATCG CCCTCCTGGT CCTCATTCTG 
CTGATTGTTT GCTTCATTAA GAGGAGCAGA GGAGGGAAAT ACCCAGTGCG TGACAACAAA 
GATGAGCACC TGAATCCTGA AGACAAGAAC GTAGAAGATG GCTCATTCGA CTACAGCGAT 
GAAGACAACA AACCACTGCC CAACAGTCAG ACCTCCCTGG ATGGCACGAT AAAGCAACAG 
GAGAGTGACG ACAGCTTGGT GGACTACGGA GAGGGTGGGG AAGGGCAGTT CAACGAGGAC 
GGCTCCTTCA TTGGCCAGTA CACAGTGAAA AAGGACAAGG AGGAAACGGA AGGCAACGAG 
AGCTCAGAAG CCACGTCCCC AGTCAATGCT ATCTACTCAT TAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154402.1
CDS	159..3686
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDLPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYSDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298916.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298916.1

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG 
CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC 
AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA 
AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG 
GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC 
ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG 
AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT 
CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG 
GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG 
GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG 
AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA 
AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC 
CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC 
ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA 
GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG 
GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG 
ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG 
GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC 
CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG 
GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC 
AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG 
CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC 
CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC 
CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG 
AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC 
AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC 
GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC 
CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC 
AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT 
GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC 
GTGCTAGAGC GACCCGACCG GCCCCGGGAC CTGGAGCTGT CAGACCTGGC TGAGAGGAGC 
GTGAAGCTGA CATGGATTCC TGGCGATGAC AACAACAGCC CCATCACAGA CTACATCGTC 
CAGTTTGAGG AGGACCGCTT CCAGCCTGGC ACGTGGCACA ACCACTCCAG GTATCCTGGG 
AATGTCAACT CGGCCCTCCT GAGCCTCTCT CCTTACGTCA ACTACCAATT TCGGGTGATT 
GCAGTGAACG ACGTGGGCAG CAGCCTGCCC AGCATGCCCT CAGAACGATA CCAGACCAGC 
GGGGCACGAC CTGAAATTAA CCCAACAGGA GTTCAAGGAG CAGGGACCCA AAAAAACAAC 
ATGGAGATAA CCTGGACGCC TCTGAATGCA ACTCAAGCCT ATGGGCCCAA CCTCCGTTAC 
ATCGTGCGGT GGAGGCGAAG GGACCCACGT GGGAGTTGGT ACAACGAGAC GGTGAAGGCA 
CCACGACACG TCGTCTGGAA CACACCCATC TACGTCCCCT ACGAGATCAA AGTGCAGGCA 
GAGAATGACT TTGGTAGAGC TCCAGAGCCT GAGACCTACA TCGGCTACTC AGGGGAAGAT 
TATCCCAAGG CTGCACCTAC AGATGTTAGG ATAAGAGTTT TAAACAGCAC TGCCATTGCT 
CTGACATGGA CCCGCGTGCA CCTGGACACC ATCCAGGGAC AGCTTAAGGA GTACAGAGCC 
TATTTCTGGA GAGACAGTAG TTTGCTGAAG AACCTGTGGG TCTCCAAAAA ACGGCAGTAT 
GTGAGTTTTC CTGGAGACCG AAACCGGGGC ATAGTGTCCC GGCTGTTCCC TTACAGCAAC 
TACAAGCTTG AGATGGTTGT AACCAACGGG AGAGGCGATG GGCCCCGCAG CGAAGTCAAG 
GAGTTCCCCA CACCTGAAGG AGTGCCCAGC TCCCCCAGGT ACTTAAGAAT CCGACAGCCA 
AATCTGGAAA GCATCAATTT GGAGTGGGAT CACCCAGAAC ATCCCAATGG AGTCCTCACG 
GGATACAACC TTAGATATCA AGCCTTTAAC GGATCCAAAA CGGGCAGAAC CCTGGTAGAG 
AACTTCTCTC CCAACCAGAC AAGGTTCACC GTGCAGAGGA CAGACCCCAT CTCGCGCTAT 
CGATTCTTCC TGCGTGCTCG GACACAGGTG GGAGATGGAG AAGTCATAGT GGAAGAGTCA 
CCAGCGCTCC TGAATGAAGC CACGCCAACC CCAGCTTACA CCAAGAACCA CGTGGACATC 
GCGACCCAGG GCTGGTTCAT CGGGCTGATG TGTGCCATCG CCCTCCTGGT CCTCATTCTG 
CTGATTGTTT GCTTCATTAA GAGGAGCAGA GGAGGGAAAT ACCCAGTGCG TGACAACAAA 
GATGAGCACC TGAATCCTGA AGACAAGAAC GTAGAAGATG GCTCATTCGA CTACAGCGAT 
GAAGACAACA AACCACTGCC CAACAGTCAG ACCTCCCTGG ATGGCACGAT AAAGCAACAG 
GAGAGTGACG ACAGCTTGGT GGACTACGGA GAGGGTGGGG AAGGGCAGTT CAACGAGGAC 
GGCTCCTTCA TTGGCCAGTA CACAGTGAAA AAGGACAAGG AGGAAACGGA AGGCAACGAG 
AGCTCAGAAG CCACGTCCCC AGTCAATGCT ATCTACTCAT TAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298916.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154402.1 Gallus gallus neurofascin (NFASC), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OGa46523
Clone ID Related Accession (Same CDS sequence)	XM_015298916.1 , XM_015298916.2
Accession Version	XM_015298916.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298916.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG 
CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC 
AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA 
AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG 
GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC 
ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG 
AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT 
CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG 
GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG 
GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG 
AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA 
AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC 
CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC 
ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA 
GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG 
GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG 
ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG 
GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC 
CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG 
GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC 
AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG 
CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC 
CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC 
CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG 
AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC 
AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC 
GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC 
CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC 
AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT 
GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC 
GTGCTAGAGC GACCCGACCG GCCCCGGGAC CTGGAGCTGT CAGACCTGGC TGAGAGGAGC 
GTGAAGCTGA CATGGATTCC TGGCGATGAC AACAACAGCC CCATCACAGA CTACATCGTC 
CAGTTTGAGG AGGACCGCTT CCAGCCTGGC ACGTGGCACA ACCACTCCAG GTATCCTGGG 
AATGTCAACT CGGCCCTCCT GAGCCTCTCT CCTTACGTCA ACTACCAATT TCGGGTGATT 
GCAGTGAACG ACGTGGGCAG CAGCCTGCCC AGCATGCCCT CAGAACGATA CCAGACCAGC 
GGGGCACGAC CTGAAATTAA CCCAACAGGA GTTCAAGGAG CAGGGACCCA AAAAAACAAC 
ATGGAGATAA CCTGGACGCC TCTGAATGCA ACTCAAGCCT ATGGGCCCAA CCTCCGTTAC 
ATCGTGCGGT GGAGGCGAAG GGACCCACGT GGGAGTTGGT ACAACGAGAC GGTGAAGGCA 
CCACGACACG TCGTCTGGAA CACACCCATC TACGTCCCCT ACGAGATCAA AGTGCAGGCA 
GAGAATGACT TTGGTAGAGC TCCAGAGCCT GAGACCTACA TCGGCTACTC AGGGGAAGAT 
TATCCCAAGG CTGCACCTAC AGATGTTAGG ATAAGAGTTT TAAACAGCAC TGCCATTGCT 
CTGACATGGA CCCGCGTGCA CCTGGACACC ATCCAGGGAC AGCTTAAGGA GTACAGAGCC 
TATTTCTGGA GAGACAGTAG TTTGCTGAAG AACCTGTGGG TCTCCAAAAA ACGGCAGTAT 
GTGAGTTTTC CTGGAGACCG AAACCGGGGC ATAGTGTCCC GGCTGTTCCC TTACAGCAAC 
TACAAGCTTG AGATGGTTGT AACCAACGGG AGAGGCGATG GGCCCCGCAG CGAAGTCAAG 
GAGTTCCCCA CACCTGAAGG AGTGCCCAGC TCCCCCAGGT ACTTAAGAAT CCGACAGCCA 
AATCTGGAAA GCATCAATTT GGAGTGGGAT CACCCAGAAC ATCCCAATGG AGTCCTCACG 
GGATACAACC TTAGATATCA AGCCTTTAAC GGATCCAAAA CGGGCAGAAC CCTGGTAGAG 
AACTTCTCTC CCAACCAGAC AAGGTTCACC GTGCAGAGGA CAGACCCCAT CTCGCGCTAT 
CGATTCTTCC TGCGTGCTCG GACACAGGTG GGAGATGGAG AAGTCATAGT GGAAGAGTCA 
CCAGCGCTCC TGAATGAAGC CACGCCAACC CCAGCTTACA CCAAGAACCA CGTGGACATC 
GCGACCCAGG GCTGGTTCAT CGGGCTGATG TGTGCCATCG CCCTCCTGGT CCTCATTCTG 
CTGATTGTTT GCTTCATTAA GAGGAGCAGA GGAGGGAAAT ACCCAGTGCG TGACAACAAA 
GATGAGCACC TGAATCCTGA AGACAAGAAC GTAGAAGATG GCTCATTCGA CTACAGCGAT 
GAAGACAACA AACCACTGCC CAACAGTCAG ACCTCCCTGG ATGGCACGAT AAAGCAACAG 
GAGAGTGACG ACAGCTTGGT GGACTACGGA GAGGGTGGGG AAGGGCAGTT CAACGAGGAC 
GGCTCCTTCA TTGGCCAGTA CACAGTGAAA AAGGACAAGG AGGAAACGGA AGGCAACGAG 
AGCTCAGAAG CCACGTCCCC AGTCAATGCT ATCTACTCAT TAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154402.1
CDS	169..3696
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDLPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYSDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298916.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298916.2

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG 
CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC 
AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA 
AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG 
GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC 
ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG 
AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT 
CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG 
GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG 
GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG 
AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA 
AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC 
CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC 
ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA 
GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG 
GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG 
ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG 
GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC 
CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG 
GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC 
AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG 
CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC 
CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC 
CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG 
AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC 
AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC 
GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC 
CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC 
AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT 
GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC 
GTGCTAGAGC GACCCGACCG GCCCCGGGAC CTGGAGCTGT CAGACCTGGC TGAGAGGAGC 
GTGAAGCTGA CATGGATTCC TGGCGATGAC AACAACAGCC CCATCACAGA CTACATCGTC 
CAGTTTGAGG AGGACCGCTT CCAGCCTGGC ACGTGGCACA ACCACTCCAG GTATCCTGGG 
AATGTCAACT CGGCCCTCCT GAGCCTCTCT CCTTACGTCA ACTACCAATT TCGGGTGATT 
GCAGTGAACG ACGTGGGCAG CAGCCTGCCC AGCATGCCCT CAGAACGATA CCAGACCAGC 
GGGGCACGAC CTGAAATTAA CCCAACAGGA GTTCAAGGAG CAGGGACCCA AAAAAACAAC 
ATGGAGATAA CCTGGACGCC TCTGAATGCA ACTCAAGCCT ATGGGCCCAA CCTCCGTTAC 
ATCGTGCGGT GGAGGCGAAG GGACCCACGT GGGAGTTGGT ACAACGAGAC GGTGAAGGCA 
CCACGACACG TCGTCTGGAA CACACCCATC TACGTCCCCT ACGAGATCAA AGTGCAGGCA 
GAGAATGACT TTGGTAGAGC TCCAGAGCCT GAGACCTACA TCGGCTACTC AGGGGAAGAT 
TATCCCAAGG CTGCACCTAC AGATGTTAGG ATAAGAGTTT TAAACAGCAC TGCCATTGCT 
CTGACATGGA CCCGCGTGCA CCTGGACACC ATCCAGGGAC AGCTTAAGGA GTACAGAGCC 
TATTTCTGGA GAGACAGTAG TTTGCTGAAG AACCTGTGGG TCTCCAAAAA ACGGCAGTAT 
GTGAGTTTTC CTGGAGACCG AAACCGGGGC ATAGTGTCCC GGCTGTTCCC TTACAGCAAC 
TACAAGCTTG AGATGGTTGT AACCAACGGG AGAGGCGATG GGCCCCGCAG CGAAGTCAAG 
GAGTTCCCCA CACCTGAAGG AGTGCCCAGC TCCCCCAGGT ACTTAAGAAT CCGACAGCCA 
AATCTGGAAA GCATCAATTT GGAGTGGGAT CACCCAGAAC ATCCCAATGG AGTCCTCACG 
GGATACAACC TTAGATATCA AGCCTTTAAC GGATCCAAAA CGGGCAGAAC CCTGGTAGAG 
AACTTCTCTC CCAACCAGAC AAGGTTCACC GTGCAGAGGA CAGACCCCAT CTCGCGCTAT 
CGATTCTTCC TGCGTGCTCG GACACAGGTG GGAGATGGAG AAGTCATAGT GGAAGAGTCA 
CCAGCGCTCC TGAATGAAGC CACGCCAACC CCAGCTTACA CCAAGAACCA CGTGGACATC 
GCGACCCAGG GCTGGTTCAT CGGGCTGATG TGTGCCATCG CCCTCCTGGT CCTCATTCTG 
CTGATTGTTT GCTTCATTAA GAGGAGCAGA GGAGGGAAAT ACCCAGTGCG TGACAACAAA 
GATGAGCACC TGAATCCTGA AGACAAGAAC GTAGAAGATG GCTCATTCGA CTACAGCGAT 
GAAGACAACA AACCACTGCC CAACAGTCAG ACCTCCCTGG ATGGCACGAT AAAGCAACAG 
GAGAGTGACG ACAGCTTGGT GGACTACGGA GAGGGTGGGG AAGGGCAGTT CAACGAGGAC 
GGCTCCTTCA TTGGCCAGTA CACAGTGAAA AAGGACAAGG AGGAAACGGA AGGCAACGAG 
AGCTCAGAAG CCACGTCCCC AGTCAATGCT ATCTACTCAT TAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298917.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154403.1 Gallus gallus neurofascin (NFASC), transcript variant X16, mRNA.

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CloneID	OGa46524
Clone ID Related Accession (Same CDS sequence)	XM_015298917.1 , XM_015298917.2
Accession Version	XM_015298917.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG 
CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC 
AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA 
AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG 
GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC 
ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG 
AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT 
CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG 
GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG 
GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG 
AAAAATCCCT ACACCCTCAA GGTGAAAACC ACCCGAGGGG TAACGGAAAC AACACCCAGC 
TTCATGTACC CATATGGGAC CTCCAGCAGC CAGATGGTGC TCCGAGGGGT GGACCTCTTG 
CTGGAGTGCA TTGCATCAGG AGTACCAGCA CCAGACATCA TGTGGTACAA GAAAGGAGGT 
GAGCTCCCAG CAGGCAAAAC CAAGCTGGAA AACTTTAACA AGGCCCTTCG TATCTCCAAC 
GTCTCGGAGG AAGACTCTGG GGAGTATTTC TGCCTGGCAT CCAACAAGAT GGGCAGCATC 
CGCCACACGA TCTCGGTGAG AGTGAAGGCT GCCCCGTATT GGCTGGATGA GCCACAGAAT 
CTCATTTTGG CCCCTGGTGA GGACGGCAGG TTGGTGTGTC GAGCCAATGG GAACCCCAAG 
CCTTCAATCC AGTGGTTGGT GAATGGAGAG CCCATTGAAG GTTCTCCACC CAACCCAAGC 
AGAGAGGTGG CTGGAGATAC CATTGTGTTT CGAGACACGC AGATCGGCAG CAGCGCTGTG 
TACCAATGCA ATGCTTCCAA CGAGCACGGC TACCTCCTTG CCAATGCCTT TGTCAGTGTC 
CTGGATGTGC CACCACGGAT ACTGGCCCCA CGCAACCAGC TCATCAAAGT CATCCAGTAC 
AACAGGACCC GGCTGGACTG CCCTTTTTTC GGCTCACCCA TCCCCACCCT GCGATGGTTT 
AAGAACGGCC AGGGGAACAT GCTGGATGGA GGGAACTACA AGGCGCATGA GAACGGGAGC 
TTGGAGATGA GCATGGCTCG GAAGGAGGAT CAGGGCATCT ACACCTGTGT CGCCACCAAC 
ATCCTGGGCA AAGTGGAGGC CCAGGTTCGC CTGGAAGTCA AAGACCCTAC CAGGATTGTG 
AGAGGCCCCG AAGATCAGGT GGTGAAGAGG GGCTCCATGC CTCGCCTGCA CTGCCGGGTG 
AAGCACGACC CAACACTGAA GCTCACGGTC ACCTGGCTGA AGGACGACGC TCCTCTCTAC 
ATTGGGAACA GGATGAAAAA AGAAGATGAT GGTCTGACAA TATATGGCGT GGCTGAGAAG 
GACCAGGGTG ACTACACCTG CGTGGCCAGC ACAGAGCTGG ACAAGGACTC AGCTAAAGCC 
TACCTCACCG TGCTAGAGCG ACCCGACCGG CCCCGGGACC TGGAGCTGTC AGACCTGGCT 
GAGAGGAGCG TGAAGCTGAC ATGGATTCCT GGCGATGACA ACAACAGCCC CATCACAGAC 
TACATCGTCC AGTTTGAGGA GGACCGCTTC CAGCCTGGCA CGTGGCACAA CCACTCCAGG 
TATCCTGGGA ATGTCAACTC GGCCCTCCTG AGCCTCTCTC CTTACGTCAA CTACCAATTT 
CGGGTGATTG CAGTGAACGA CGTGGGCAGC AGCCTGCCCA GCATGCCCTC AGAACGATAC 
CAGACCAGCG GGGCACGACC TGAAATTAAC CCAACAGGAG TTCAAGGAGC AGGGACCCAA 
AAAAACAACA TGGAGATAAC CTGGACGCCT CTGAATGCAA CTCAAGCCTA TGGGCCCAAC 
CTCCGTTACA TCGTGCGGTG GAGGCGAAGG GACCCACGTG GGAGTTGGTA CAACGAGACG 
GTGAAGGCAC CACGACACGT CGTCTGGAAC ACACCCATCT ACGTCCCCTA CGAGATCAAA 
GTGCAGGCAG AGAATGACTT TGGTAGAGCT CCAGAGCCTG AGACCTACAT CGGCTACTCA 
GGGGAAGATT ATCCCAAGGC TGCACCTACA GATGTTAGGA TAAGAGTTTT AAACAGCACT 
GCCATTGCTC TGACATGGAC CCGCGTGCAC CTGGACACCA TCCAGGGACA GCTTAAGGAG 
TACAGAGCCT ATTTCTGGAG AGACAGTAGT TTGCTGAAGA ACCTGTGGGT CTCCAAAAAA 
CGGCAGTATG TGAGTTTTCC TGGAGACCGA AACCGGGGCA TAGTGTCCCG GCTGTTCCCT 
TACAGCAACT ACAAGCTTGA GATGGTTGTA ACCAACGGGA GAGGCGATGG GCCCCGCAGC 
GAAGTCAAGG AGTTCCCCAC ACCTGAAGGA GTGCCCAGCT CCCCCAGGTA CTTAAGAATC 
CGACAGCCAA ATCTGGAAAG CATCAATTTG GAGTGGGATC ACCCAGAACA TCCCAATGGA 
GTCCTCACGG GATACAACCT TAGATATCAA GCCTTTAACG GATCCAAAAC GGGCAGAACC 
CTGGTAGAGA ACTTCTCTCC CAACCAGACA AGGTTCACCG TGCAGAGGAC AGACCCCATC 
TCGCGCTATC GATTCTTCCT GCGTGCTCGG ACACAGGTGG GAGATGGAGA AGTCATAGTG 
GAAGAGTCAC CAGCGCTCCT GAATGAAGCC ACGCCAACCC CAGCTTACAC CAAGAACCAC 
GTGGACATCG CGACCCAGGG CTGGTTCATC GGGCTGATGT GTGCCATCGC CCTCCTGGTC 
CTCATTCTGC TGATTGTTTG CTTCATTAAG AGGAGCAGAG GAGGGAAATA CCCAGTGCGT 
GACAACAAAG ATGAGCACCT GAATCCTGAA GACAAGAACG TAGAAGATGG CTCATTCGAC 
TACAGCGATG AAGACAACAA ACCACTGCCC AACAGTCAGA CCTCCCTGGA TGGCACGATA 
AAGCAACAGG AGAGTGACGA CAGCTTGGTG GACTACGGAG AGGGTGGGGA AGGGCAGTTC 
AACGAGGACG GCTCCTTCAT TGGCCAGTAC ACAGTGAAAA AGGACAAGGA GGAAACGGAA 
GGCAACGAGA GCTCAGAAGC CACGTCCCCA GTCAATGCTA TCTACTCATT AGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154403.1
CDS	159..3635
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDLPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTTRGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYSDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298917.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298917.1

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG 
CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC 
AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA 
AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG 
GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC 
ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG 
AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT 
CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG 
GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG 
GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG 
AAAAATCCCT ACACCCTCAA GGTGAAAACC ACCCGAGGGG TAACGGAAAC AACACCCAGC 
TTCATGTACC CATATGGGAC CTCCAGCAGC CAGATGGTGC TCCGAGGGGT GGACCTCTTG 
CTGGAGTGCA TTGCATCAGG AGTACCAGCA CCAGACATCA TGTGGTACAA GAAAGGAGGT 
GAGCTCCCAG CAGGCAAAAC CAAGCTGGAA AACTTTAACA AGGCCCTTCG TATCTCCAAC 
GTCTCGGAGG AAGACTCTGG GGAGTATTTC TGCCTGGCAT CCAACAAGAT GGGCAGCATC 
CGCCACACGA TCTCGGTGAG AGTGAAGGCT GCCCCGTATT GGCTGGATGA GCCACAGAAT 
CTCATTTTGG CCCCTGGTGA GGACGGCAGG TTGGTGTGTC GAGCCAATGG GAACCCCAAG 
CCTTCAATCC AGTGGTTGGT GAATGGAGAG CCCATTGAAG GTTCTCCACC CAACCCAAGC 
AGAGAGGTGG CTGGAGATAC CATTGTGTTT CGAGACACGC AGATCGGCAG CAGCGCTGTG 
TACCAATGCA ATGCTTCCAA CGAGCACGGC TACCTCCTTG CCAATGCCTT TGTCAGTGTC 
CTGGATGTGC CACCACGGAT ACTGGCCCCA CGCAACCAGC TCATCAAAGT CATCCAGTAC 
AACAGGACCC GGCTGGACTG CCCTTTTTTC GGCTCACCCA TCCCCACCCT GCGATGGTTT 
AAGAACGGCC AGGGGAACAT GCTGGATGGA GGGAACTACA AGGCGCATGA GAACGGGAGC 
TTGGAGATGA GCATGGCTCG GAAGGAGGAT CAGGGCATCT ACACCTGTGT CGCCACCAAC 
ATCCTGGGCA AAGTGGAGGC CCAGGTTCGC CTGGAAGTCA AAGACCCTAC CAGGATTGTG 
AGAGGCCCCG AAGATCAGGT GGTGAAGAGG GGCTCCATGC CTCGCCTGCA CTGCCGGGTG 
AAGCACGACC CAACACTGAA GCTCACGGTC ACCTGGCTGA AGGACGACGC TCCTCTCTAC 
ATTGGGAACA GGATGAAAAA AGAAGATGAT GGTCTGACAA TATATGGCGT GGCTGAGAAG 
GACCAGGGTG ACTACACCTG CGTGGCCAGC ACAGAGCTGG ACAAGGACTC AGCTAAAGCC 
TACCTCACCG TGCTAGAGCG ACCCGACCGG CCCCGGGACC TGGAGCTGTC AGACCTGGCT 
GAGAGGAGCG TGAAGCTGAC ATGGATTCCT GGCGATGACA ACAACAGCCC CATCACAGAC 
TACATCGTCC AGTTTGAGGA GGACCGCTTC CAGCCTGGCA CGTGGCACAA CCACTCCAGG 
TATCCTGGGA ATGTCAACTC GGCCCTCCTG AGCCTCTCTC CTTACGTCAA CTACCAATTT 
CGGGTGATTG CAGTGAACGA CGTGGGCAGC AGCCTGCCCA GCATGCCCTC AGAACGATAC 
CAGACCAGCG GGGCACGACC TGAAATTAAC CCAACAGGAG TTCAAGGAGC AGGGACCCAA 
AAAAACAACA TGGAGATAAC CTGGACGCCT CTGAATGCAA CTCAAGCCTA TGGGCCCAAC 
CTCCGTTACA TCGTGCGGTG GAGGCGAAGG GACCCACGTG GGAGTTGGTA CAACGAGACG 
GTGAAGGCAC CACGACACGT CGTCTGGAAC ACACCCATCT ACGTCCCCTA CGAGATCAAA 
GTGCAGGCAG AGAATGACTT TGGTAGAGCT CCAGAGCCTG AGACCTACAT CGGCTACTCA 
GGGGAAGATT ATCCCAAGGC TGCACCTACA GATGTTAGGA TAAGAGTTTT AAACAGCACT 
GCCATTGCTC TGACATGGAC CCGCGTGCAC CTGGACACCA TCCAGGGACA GCTTAAGGAG 
TACAGAGCCT ATTTCTGGAG AGACAGTAGT TTGCTGAAGA ACCTGTGGGT CTCCAAAAAA 
CGGCAGTATG TGAGTTTTCC TGGAGACCGA AACCGGGGCA TAGTGTCCCG GCTGTTCCCT 
TACAGCAACT ACAAGCTTGA GATGGTTGTA ACCAACGGGA GAGGCGATGG GCCCCGCAGC 
GAAGTCAAGG AGTTCCCCAC ACCTGAAGGA GTGCCCAGCT CCCCCAGGTA CTTAAGAATC 
CGACAGCCAA ATCTGGAAAG CATCAATTTG GAGTGGGATC ACCCAGAACA TCCCAATGGA 
GTCCTCACGG GATACAACCT TAGATATCAA GCCTTTAACG GATCCAAAAC GGGCAGAACC 
CTGGTAGAGA ACTTCTCTCC CAACCAGACA AGGTTCACCG TGCAGAGGAC AGACCCCATC 
TCGCGCTATC GATTCTTCCT GCGTGCTCGG ACACAGGTGG GAGATGGAGA AGTCATAGTG 
GAAGAGTCAC CAGCGCTCCT GAATGAAGCC ACGCCAACCC CAGCTTACAC CAAGAACCAC 
GTGGACATCG CGACCCAGGG CTGGTTCATC GGGCTGATGT GTGCCATCGC CCTCCTGGTC 
CTCATTCTGC TGATTGTTTG CTTCATTAAG AGGAGCAGAG GAGGGAAATA CCCAGTGCGT 
GACAACAAAG ATGAGCACCT GAATCCTGAA GACAAGAACG TAGAAGATGG CTCATTCGAC 
TACAGCGATG AAGACAACAA ACCACTGCCC AACAGTCAGA CCTCCCTGGA TGGCACGATA 
AAGCAACAGG AGAGTGACGA CAGCTTGGTG GACTACGGAG AGGGTGGGGA AGGGCAGTTC 
AACGAGGACG GCTCCTTCAT TGGCCAGTAC ACAGTGAAAA AGGACAAGGA GGAAACGGAA 
GGCAACGAGA GCTCAGAAGC CACGTCCCCA GTCAATGCTA TCTACTCATT AGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298917.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154403.1 Gallus gallus neurofascin (NFASC), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OGa46524
Clone ID Related Accession (Same CDS sequence)	XM_015298917.1 , XM_015298917.2
Accession Version	XM_015298917.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298917.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG 
CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC 
AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA 
AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG 
GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC 
ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG 
AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT 
CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG 
GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG 
GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG 
AAAAATCCCT ACACCCTCAA GGTGAAAACC ACCCGAGGGG TAACGGAAAC AACACCCAGC 
TTCATGTACC CATATGGGAC CTCCAGCAGC CAGATGGTGC TCCGAGGGGT GGACCTCTTG 
CTGGAGTGCA TTGCATCAGG AGTACCAGCA CCAGACATCA TGTGGTACAA GAAAGGAGGT 
GAGCTCCCAG CAGGCAAAAC CAAGCTGGAA AACTTTAACA AGGCCCTTCG TATCTCCAAC 
GTCTCGGAGG AAGACTCTGG GGAGTATTTC TGCCTGGCAT CCAACAAGAT GGGCAGCATC 
CGCCACACGA TCTCGGTGAG AGTGAAGGCT GCCCCGTATT GGCTGGATGA GCCACAGAAT 
CTCATTTTGG CCCCTGGTGA GGACGGCAGG TTGGTGTGTC GAGCCAATGG GAACCCCAAG 
CCTTCAATCC AGTGGTTGGT GAATGGAGAG CCCATTGAAG GTTCTCCACC CAACCCAAGC 
AGAGAGGTGG CTGGAGATAC CATTGTGTTT CGAGACACGC AGATCGGCAG CAGCGCTGTG 
TACCAATGCA ATGCTTCCAA CGAGCACGGC TACCTCCTTG CCAATGCCTT TGTCAGTGTC 
CTGGATGTGC CACCACGGAT ACTGGCCCCA CGCAACCAGC TCATCAAAGT CATCCAGTAC 
AACAGGACCC GGCTGGACTG CCCTTTTTTC GGCTCACCCA TCCCCACCCT GCGATGGTTT 
AAGAACGGCC AGGGGAACAT GCTGGATGGA GGGAACTACA AGGCGCATGA GAACGGGAGC 
TTGGAGATGA GCATGGCTCG GAAGGAGGAT CAGGGCATCT ACACCTGTGT CGCCACCAAC 
ATCCTGGGCA AAGTGGAGGC CCAGGTTCGC CTGGAAGTCA AAGACCCTAC CAGGATTGTG 
AGAGGCCCCG AAGATCAGGT GGTGAAGAGG GGCTCCATGC CTCGCCTGCA CTGCCGGGTG 
AAGCACGACC CAACACTGAA GCTCACGGTC ACCTGGCTGA AGGACGACGC TCCTCTCTAC 
ATTGGGAACA GGATGAAAAA AGAAGATGAT GGTCTGACAA TATATGGCGT GGCTGAGAAG 
GACCAGGGTG ACTACACCTG CGTGGCCAGC ACAGAGCTGG ACAAGGACTC AGCTAAAGCC 
TACCTCACCG TGCTAGAGCG ACCCGACCGG CCCCGGGACC TGGAGCTGTC AGACCTGGCT 
GAGAGGAGCG TGAAGCTGAC ATGGATTCCT GGCGATGACA ACAACAGCCC CATCACAGAC 
TACATCGTCC AGTTTGAGGA GGACCGCTTC CAGCCTGGCA CGTGGCACAA CCACTCCAGG 
TATCCTGGGA ATGTCAACTC GGCCCTCCTG AGCCTCTCTC CTTACGTCAA CTACCAATTT 
CGGGTGATTG CAGTGAACGA CGTGGGCAGC AGCCTGCCCA GCATGCCCTC AGAACGATAC 
CAGACCAGCG GGGCACGACC TGAAATTAAC CCAACAGGAG TTCAAGGAGC AGGGACCCAA 
AAAAACAACA TGGAGATAAC CTGGACGCCT CTGAATGCAA CTCAAGCCTA TGGGCCCAAC 
CTCCGTTACA TCGTGCGGTG GAGGCGAAGG GACCCACGTG GGAGTTGGTA CAACGAGACG 
GTGAAGGCAC CACGACACGT CGTCTGGAAC ACACCCATCT ACGTCCCCTA CGAGATCAAA 
GTGCAGGCAG AGAATGACTT TGGTAGAGCT CCAGAGCCTG AGACCTACAT CGGCTACTCA 
GGGGAAGATT ATCCCAAGGC TGCACCTACA GATGTTAGGA TAAGAGTTTT AAACAGCACT 
GCCATTGCTC TGACATGGAC CCGCGTGCAC CTGGACACCA TCCAGGGACA GCTTAAGGAG 
TACAGAGCCT ATTTCTGGAG AGACAGTAGT TTGCTGAAGA ACCTGTGGGT CTCCAAAAAA 
CGGCAGTATG TGAGTTTTCC TGGAGACCGA AACCGGGGCA TAGTGTCCCG GCTGTTCCCT 
TACAGCAACT ACAAGCTTGA GATGGTTGTA ACCAACGGGA GAGGCGATGG GCCCCGCAGC 
GAAGTCAAGG AGTTCCCCAC ACCTGAAGGA GTGCCCAGCT CCCCCAGGTA CTTAAGAATC 
CGACAGCCAA ATCTGGAAAG CATCAATTTG GAGTGGGATC ACCCAGAACA TCCCAATGGA 
GTCCTCACGG GATACAACCT TAGATATCAA GCCTTTAACG GATCCAAAAC GGGCAGAACC 
CTGGTAGAGA ACTTCTCTCC CAACCAGACA AGGTTCACCG TGCAGAGGAC AGACCCCATC 
TCGCGCTATC GATTCTTCCT GCGTGCTCGG ACACAGGTGG GAGATGGAGA AGTCATAGTG 
GAAGAGTCAC CAGCGCTCCT GAATGAAGCC ACGCCAACCC CAGCTTACAC CAAGAACCAC 
GTGGACATCG CGACCCAGGG CTGGTTCATC GGGCTGATGT GTGCCATCGC CCTCCTGGTC 
CTCATTCTGC TGATTGTTTG CTTCATTAAG AGGAGCAGAG GAGGGAAATA CCCAGTGCGT 
GACAACAAAG ATGAGCACCT GAATCCTGAA GACAAGAACG TAGAAGATGG CTCATTCGAC 
TACAGCGATG AAGACAACAA ACCACTGCCC AACAGTCAGA CCTCCCTGGA TGGCACGATA 
AAGCAACAGG AGAGTGACGA CAGCTTGGTG GACTACGGAG AGGGTGGGGA AGGGCAGTTC 
AACGAGGACG GCTCCTTCAT TGGCCAGTAC ACAGTGAAAA AGGACAAGGA GGAAACGGAA 
GGCAACGAGA GCTCAGAAGC CACGTCCCCA GTCAATGCTA TCTACTCATT AGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154403.1
CDS	169..3645
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDLPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTTRGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYSDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298917.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298917.2

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG 
CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC 
AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA 
AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG 
GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC 
ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG 
AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT 
CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG 
GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG 
GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG 
AAAAATCCCT ACACCCTCAA GGTGAAAACC ACCCGAGGGG TAACGGAAAC AACACCCAGC 
TTCATGTACC CATATGGGAC CTCCAGCAGC CAGATGGTGC TCCGAGGGGT GGACCTCTTG 
CTGGAGTGCA TTGCATCAGG AGTACCAGCA CCAGACATCA TGTGGTACAA GAAAGGAGGT 
GAGCTCCCAG CAGGCAAAAC CAAGCTGGAA AACTTTAACA AGGCCCTTCG TATCTCCAAC 
GTCTCGGAGG AAGACTCTGG GGAGTATTTC TGCCTGGCAT CCAACAAGAT GGGCAGCATC 
CGCCACACGA TCTCGGTGAG AGTGAAGGCT GCCCCGTATT GGCTGGATGA GCCACAGAAT 
CTCATTTTGG CCCCTGGTGA GGACGGCAGG TTGGTGTGTC GAGCCAATGG GAACCCCAAG 
CCTTCAATCC AGTGGTTGGT GAATGGAGAG CCCATTGAAG GTTCTCCACC CAACCCAAGC 
AGAGAGGTGG CTGGAGATAC CATTGTGTTT CGAGACACGC AGATCGGCAG CAGCGCTGTG 
TACCAATGCA ATGCTTCCAA CGAGCACGGC TACCTCCTTG CCAATGCCTT TGTCAGTGTC 
CTGGATGTGC CACCACGGAT ACTGGCCCCA CGCAACCAGC TCATCAAAGT CATCCAGTAC 
AACAGGACCC GGCTGGACTG CCCTTTTTTC GGCTCACCCA TCCCCACCCT GCGATGGTTT 
AAGAACGGCC AGGGGAACAT GCTGGATGGA GGGAACTACA AGGCGCATGA GAACGGGAGC 
TTGGAGATGA GCATGGCTCG GAAGGAGGAT CAGGGCATCT ACACCTGTGT CGCCACCAAC 
ATCCTGGGCA AAGTGGAGGC CCAGGTTCGC CTGGAAGTCA AAGACCCTAC CAGGATTGTG 
AGAGGCCCCG AAGATCAGGT GGTGAAGAGG GGCTCCATGC CTCGCCTGCA CTGCCGGGTG 
AAGCACGACC CAACACTGAA GCTCACGGTC ACCTGGCTGA AGGACGACGC TCCTCTCTAC 
ATTGGGAACA GGATGAAAAA AGAAGATGAT GGTCTGACAA TATATGGCGT GGCTGAGAAG 
GACCAGGGTG ACTACACCTG CGTGGCCAGC ACAGAGCTGG ACAAGGACTC AGCTAAAGCC 
TACCTCACCG TGCTAGAGCG ACCCGACCGG CCCCGGGACC TGGAGCTGTC AGACCTGGCT 
GAGAGGAGCG TGAAGCTGAC ATGGATTCCT GGCGATGACA ACAACAGCCC CATCACAGAC 
TACATCGTCC AGTTTGAGGA GGACCGCTTC CAGCCTGGCA CGTGGCACAA CCACTCCAGG 
TATCCTGGGA ATGTCAACTC GGCCCTCCTG AGCCTCTCTC CTTACGTCAA CTACCAATTT 
CGGGTGATTG CAGTGAACGA CGTGGGCAGC AGCCTGCCCA GCATGCCCTC AGAACGATAC 
CAGACCAGCG GGGCACGACC TGAAATTAAC CCAACAGGAG TTCAAGGAGC AGGGACCCAA 
AAAAACAACA TGGAGATAAC CTGGACGCCT CTGAATGCAA CTCAAGCCTA TGGGCCCAAC 
CTCCGTTACA TCGTGCGGTG GAGGCGAAGG GACCCACGTG GGAGTTGGTA CAACGAGACG 
GTGAAGGCAC CACGACACGT CGTCTGGAAC ACACCCATCT ACGTCCCCTA CGAGATCAAA 
GTGCAGGCAG AGAATGACTT TGGTAGAGCT CCAGAGCCTG AGACCTACAT CGGCTACTCA 
GGGGAAGATT ATCCCAAGGC TGCACCTACA GATGTTAGGA TAAGAGTTTT AAACAGCACT 
GCCATTGCTC TGACATGGAC CCGCGTGCAC CTGGACACCA TCCAGGGACA GCTTAAGGAG 
TACAGAGCCT ATTTCTGGAG AGACAGTAGT TTGCTGAAGA ACCTGTGGGT CTCCAAAAAA 
CGGCAGTATG TGAGTTTTCC TGGAGACCGA AACCGGGGCA TAGTGTCCCG GCTGTTCCCT 
TACAGCAACT ACAAGCTTGA GATGGTTGTA ACCAACGGGA GAGGCGATGG GCCCCGCAGC 
GAAGTCAAGG AGTTCCCCAC ACCTGAAGGA GTGCCCAGCT CCCCCAGGTA CTTAAGAATC 
CGACAGCCAA ATCTGGAAAG CATCAATTTG GAGTGGGATC ACCCAGAACA TCCCAATGGA 
GTCCTCACGG GATACAACCT TAGATATCAA GCCTTTAACG GATCCAAAAC GGGCAGAACC 
CTGGTAGAGA ACTTCTCTCC CAACCAGACA AGGTTCACCG TGCAGAGGAC AGACCCCATC 
TCGCGCTATC GATTCTTCCT GCGTGCTCGG ACACAGGTGG GAGATGGAGA AGTCATAGTG 
GAAGAGTCAC CAGCGCTCCT GAATGAAGCC ACGCCAACCC CAGCTTACAC CAAGAACCAC 
GTGGACATCG CGACCCAGGG CTGGTTCATC GGGCTGATGT GTGCCATCGC CCTCCTGGTC 
CTCATTCTGC TGATTGTTTG CTTCATTAAG AGGAGCAGAG GAGGGAAATA CCCAGTGCGT 
GACAACAAAG ATGAGCACCT GAATCCTGAA GACAAGAACG TAGAAGATGG CTCATTCGAC 
TACAGCGATG AAGACAACAA ACCACTGCCC AACAGTCAGA CCTCCCTGGA TGGCACGATA 
AAGCAACAGG AGAGTGACGA CAGCTTGGTG GACTACGGAG AGGGTGGGGA AGGGCAGTTC 
AACGAGGACG GCTCCTTCAT TGGCCAGTAC ACAGTGAAAA AGGACAAGGA GGAAACGGAA 
GGCAACGAGA GCTCAGAAGC CACGTCCCCA GTCAATGCTA TCTACTCATT AGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298918.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154404.1 Gallus gallus neurofascin (NFASC), transcript variant X17, mRNA.

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CloneID	OGa46525
Clone ID Related Accession (Same CDS sequence)	XM_015298918.1 , XM_015298918.2
Accession Version	XM_015298918.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTTGCCC 
AGCTCCCCCA GGTACTTAAG AATCCGACAG CCAAATCTGG AAAGCATCAA TTTGGAGTGG 
GATCACCCAG AACATCCCAA TGGAGTCCTC ACGGGATACA ACCTTAGATA TCAAGCCTTT 
AACGGATCCA AAACGGGCAG AACCCTGGTA GAGAACTTCT CTCCCAACCA GACAAGGTTC 
ACCGTGCAGA GGACAGACCC CATCTCGCGC TATCGATTCT TCCTGCGTGC TCGGACACAG 
GTGGGAGATG GAGAAGTCAT AGTGGAAGAG TCACCAGCGC TCCTGAATGA AGCTTACACC 
AAGAACCACG TGGACATCGC GACCCAGGGC TGGTTCATCG GGCTGATGTG TGCCATCGCC 
CTCCTGGTCC TCATTCTGCT GATTGTTTGC TTCATTAAGA GGAGCAGAGG AGGGAAATAC 
CCAGTGCGTG ACAACAAAGA TGAGCACCTG AATCCTGAAG ACAAGAACGT AGAAGATGGC 
TCATTCGACT ACAGCGATGA AGACAACAAA CCACTGCCCA ACAGTCAGAC CTCCCTGGAT 
GGCACGATAA AGCAACAGGA GAGTGACGAC AGCTTGGTGG ACTACGGAGA GGGTGGGGAA 
GGGCAGTTCA ACGAGGACGG CTCCTTCATT GGCCAGTACA CAGTGAAAAA GGACAAGGAG 
GAAACGGAAG GCAACGAGAG CTCAGAAGCC ACGTCCCCAG TCAATGCTAT CTACTCATTA 
GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154404.1
CDS	159..3404
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDLPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYSDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298918.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298918.1

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTTGCCC 
AGCTCCCCCA GGTACTTAAG AATCCGACAG CCAAATCTGG AAAGCATCAA TTTGGAGTGG 
GATCACCCAG AACATCCCAA TGGAGTCCTC ACGGGATACA ACCTTAGATA TCAAGCCTTT 
AACGGATCCA AAACGGGCAG AACCCTGGTA GAGAACTTCT CTCCCAACCA GACAAGGTTC 
ACCGTGCAGA GGACAGACCC CATCTCGCGC TATCGATTCT TCCTGCGTGC TCGGACACAG 
GTGGGAGATG GAGAAGTCAT AGTGGAAGAG TCACCAGCGC TCCTGAATGA AGCTTACACC 
AAGAACCACG TGGACATCGC GACCCAGGGC TGGTTCATCG GGCTGATGTG TGCCATCGCC 
CTCCTGGTCC TCATTCTGCT GATTGTTTGC TTCATTAAGA GGAGCAGAGG AGGGAAATAC 
CCAGTGCGTG ACAACAAAGA TGAGCACCTG AATCCTGAAG ACAAGAACGT AGAAGATGGC 
TCATTCGACT ACAGCGATGA AGACAACAAA CCACTGCCCA ACAGTCAGAC CTCCCTGGAT 
GGCACGATAA AGCAACAGGA GAGTGACGAC AGCTTGGTGG ACTACGGAGA GGGTGGGGAA 
GGGCAGTTCA ACGAGGACGG CTCCTTCATT GGCCAGTACA CAGTGAAAAA GGACAAGGAG 
GAAACGGAAG GCAACGAGAG CTCAGAAGCC ACGTCCCCAG TCAATGCTAT CTACTCATTA 
GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298918.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154404.1 Gallus gallus neurofascin (NFASC), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OGa46525
Clone ID Related Accession (Same CDS sequence)	XM_015298918.1 , XM_015298918.2
Accession Version	XM_015298918.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298918.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTTGCCC 
AGCTCCCCCA GGTACTTAAG AATCCGACAG CCAAATCTGG AAAGCATCAA TTTGGAGTGG 
GATCACCCAG AACATCCCAA TGGAGTCCTC ACGGGATACA ACCTTAGATA TCAAGCCTTT 
AACGGATCCA AAACGGGCAG AACCCTGGTA GAGAACTTCT CTCCCAACCA GACAAGGTTC 
ACCGTGCAGA GGACAGACCC CATCTCGCGC TATCGATTCT TCCTGCGTGC TCGGACACAG 
GTGGGAGATG GAGAAGTCAT AGTGGAAGAG TCACCAGCGC TCCTGAATGA AGCTTACACC 
AAGAACCACG TGGACATCGC GACCCAGGGC TGGTTCATCG GGCTGATGTG TGCCATCGCC 
CTCCTGGTCC TCATTCTGCT GATTGTTTGC TTCATTAAGA GGAGCAGAGG AGGGAAATAC 
CCAGTGCGTG ACAACAAAGA TGAGCACCTG AATCCTGAAG ACAAGAACGT AGAAGATGGC 
TCATTCGACT ACAGCGATGA AGACAACAAA CCACTGCCCA ACAGTCAGAC CTCCCTGGAT 
GGCACGATAA AGCAACAGGA GAGTGACGAC AGCTTGGTGG ACTACGGAGA GGGTGGGGAA 
GGGCAGTTCA ACGAGGACGG CTCCTTCATT GGCCAGTACA CAGTGAAAAA GGACAAGGAG 
GAAACGGAAG GCAACGAGAG CTCAGAAGCC ACGTCCCCAG TCAATGCTAT CTACTCATTA 
GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154404.1
CDS	169..3414
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDLPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYSDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298918.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298918.2

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTTGCCC 
AGCTCCCCCA GGTACTTAAG AATCCGACAG CCAAATCTGG AAAGCATCAA TTTGGAGTGG 
GATCACCCAG AACATCCCAA TGGAGTCCTC ACGGGATACA ACCTTAGATA TCAAGCCTTT 
AACGGATCCA AAACGGGCAG AACCCTGGTA GAGAACTTCT CTCCCAACCA GACAAGGTTC 
ACCGTGCAGA GGACAGACCC CATCTCGCGC TATCGATTCT TCCTGCGTGC TCGGACACAG 
GTGGGAGATG GAGAAGTCAT AGTGGAAGAG TCACCAGCGC TCCTGAATGA AGCTTACACC 
AAGAACCACG TGGACATCGC GACCCAGGGC TGGTTCATCG GGCTGATGTG TGCCATCGCC 
CTCCTGGTCC TCATTCTGCT GATTGTTTGC TTCATTAAGA GGAGCAGAGG AGGGAAATAC 
CCAGTGCGTG ACAACAAAGA TGAGCACCTG AATCCTGAAG ACAAGAACGT AGAAGATGGC 
TCATTCGACT ACAGCGATGA AGACAACAAA CCACTGCCCA ACAGTCAGAC CTCCCTGGAT 
GGCACGATAA AGCAACAGGA GAGTGACGAC AGCTTGGTGG ACTACGGAGA GGGTGGGGAA 
GGGCAGTTCA ACGAGGACGG CTCCTTCATT GGCCAGTACA CAGTGAAAAA GGACAAGGAG 
GAAACGGAAG GCAACGAGAG CTCAGAAGCC ACGTCCCCAG TCAATGCTAT CTACTCATTA 
GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298920.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154406.1 Gallus gallus neurofascin (NFASC), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OGa46527
Clone ID Related Accession (Same CDS sequence)	XM_015298920.1 , XM_015298920.2
Accession Version	XM_015298920.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA 
ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC 
CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG 
TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG 
GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC 
AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG 
CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA 
GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT 
TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG 
ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC 
AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC 
ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC 
CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG 
GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC 
ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA 
GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT 
CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG 
GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA 
TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC 
AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC 
TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG 
AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC 
GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT 
GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG 
ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC 
AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC 
AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT 
TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG 
GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG 
GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA 
GATTATCCCA AGGCTGCACC TACAGATGTT AGGATAAGAG TTTTAAACAG CACTGCCATT 
GCTCTGACAT GGACCCGCGT GCACCTGGAC ACCATCCAGG GACAGCTTAA GGAGTACAGA 
GCCTATTTCT GGAGAGACAG TAGTTTGCTG AAGAACCTGT GGGTCTCCAA AAAACGGCAG 
TATGTGAGTT TTCCTGGAGA CCGAAACCGG GGCATAGTGT CCCGGCTGTT CCCTTACAGC 
AACTACAAGC TTGAGATGGT TGTAACCAAC GGGAGAGGCG ATGGGCCCCG CAGCGAAGTC 
AAGGAGTTCC CCACACCTGA AGGAGTGCCC AGCTCCCCCA GGTACTTAAG AATCCGACAG 
CCAAATCTGG AAAGCATCAA TTTGGAGTGG GATCACCCAG AACATCCCAA TGGAGTCCTC 
ACGGGATACA ACCTTAGATA TCAAGCCTTT AACGGATCCA AAACGGGCAG AACCCTGGTA 
GAGAACTTCT CTCCCAACCA GACAAGGTTC ACCGTGCAGA GGACAGACCC CATCTCGCGC 
TATCGATTCT TCCTGCGTGC TCGGACACAG GTGGGAGATG GAGAAGTCAT AGTGGAAGAG 
TCACCAGCGC TCCTGAATGA AGCCACGCCA ACCCCAGCCT CCACCTGGTT GCCTCCACCT 
ACAACCGAGC TAACTCCAGC AGCCACCATT GCCACCACAA CCACCACTGC CACCCCTACT 
ACTGAAACCC CTCCTACTGA AATCCCTACT ACTGCCATCC CTACCACCAC CACTACTACA 
ACCACCACAG CTGCCAGCAC CGTCGCAAGC ACTACAACAA CTGCAGAGAG GGCTGCGGCA 
GCCACCACAA AACAGGAGCT GGCCACCAAT GGATCGTCCA TATGGGACAT AAGAGCTTGG 
GCTAATAGTA ACTGGGCAAA CATCACCTGG AGCCACAATT ACTCTGCAGG AACTGACTTT 
GTGGTTAAGT ATATCACCAG TAACAAGACA GAGAAATCTA TCCCCGTTAA AGCTCAGACC 
CCCTCCTCTG TGCAGCTGGC CAATCTGACG CCGGGGATGG TGTACAAGCT GTGGGTGTTC 
CCCATATGGT CTAGCCCCAG CGAGCACTCG TACATAACCT TTACCACCAG CTCAGCTTAC 
ACCAAGAACC ACGTGGACAT CGCGACCCAG GGCTGGTTCA TCGGGCTGAT GTGTGCCATC 
GCCCTCCTGG TCCTCATTCT GCTGATTGTT TGCTTCATTA AGAGGAGCAG AGGAGGGAAA 
TACCCAGTGC GTGACAACAA AGATGAGCAC CTGAATCCTG AAGACAAGAA CGTAGAAGAT 
GGCTCATTCG ACTACAGCGA TGAAGACAAC AAACCACTGC CCAACAGTCA GACCTCCCTG 
GATGGCACGA TAAAGCAACA GGAGAGTGAC GACAGCTTGG TGGACTACGG AGAGGGTGGG 
GAAGGGCAGT TCAACGAGGA CGGCTCCTTC ATTGGCCAGT ACACAGTGAA AAAGGACAAG 
GAGGAAACGG AAGGCAACGA GAGCTCAGAA GCCACGTCCC CAGTCAATGC TATCTACTCA 
TTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154406.1
CDS	159..4187
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTTRGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELATNGSSIWDIRAWANSNWANITWSHNYSAGTDFVVKYITSNKTEKSIPVKAQTPSSVQLANLTPGMVYKLWVFPIWSSPSEHSYITFTTSSAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYSDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298920.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298920.1

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA 
ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC 
CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG 
TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG 
GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC 
AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG 
CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA 
GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT 
TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG 
ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC 
AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC 
ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC 
CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG 
GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC 
ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA 
GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT 
CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG 
GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA 
TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC 
AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC 
TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG 
AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC 
GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT 
GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG 
ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC 
AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC 
AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT 
TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG 
GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG 
GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA 
GATTATCCCA AGGCTGCACC TACAGATGTT AGGATAAGAG TTTTAAACAG CACTGCCATT 
GCTCTGACAT GGACCCGCGT GCACCTGGAC ACCATCCAGG GACAGCTTAA GGAGTACAGA 
GCCTATTTCT GGAGAGACAG TAGTTTGCTG AAGAACCTGT GGGTCTCCAA AAAACGGCAG 
TATGTGAGTT TTCCTGGAGA CCGAAACCGG GGCATAGTGT CCCGGCTGTT CCCTTACAGC 
AACTACAAGC TTGAGATGGT TGTAACCAAC GGGAGAGGCG ATGGGCCCCG CAGCGAAGTC 
AAGGAGTTCC CCACACCTGA AGGAGTGCCC AGCTCCCCCA GGTACTTAAG AATCCGACAG 
CCAAATCTGG AAAGCATCAA TTTGGAGTGG GATCACCCAG AACATCCCAA TGGAGTCCTC 
ACGGGATACA ACCTTAGATA TCAAGCCTTT AACGGATCCA AAACGGGCAG AACCCTGGTA 
GAGAACTTCT CTCCCAACCA GACAAGGTTC ACCGTGCAGA GGACAGACCC CATCTCGCGC 
TATCGATTCT TCCTGCGTGC TCGGACACAG GTGGGAGATG GAGAAGTCAT AGTGGAAGAG 
TCACCAGCGC TCCTGAATGA AGCCACGCCA ACCCCAGCCT CCACCTGGTT GCCTCCACCT 
ACAACCGAGC TAACTCCAGC AGCCACCATT GCCACCACAA CCACCACTGC CACCCCTACT 
ACTGAAACCC CTCCTACTGA AATCCCTACT ACTGCCATCC CTACCACCAC CACTACTACA 
ACCACCACAG CTGCCAGCAC CGTCGCAAGC ACTACAACAA CTGCAGAGAG GGCTGCGGCA 
GCCACCACAA AACAGGAGCT GGCCACCAAT GGATCGTCCA TATGGGACAT AAGAGCTTGG 
GCTAATAGTA ACTGGGCAAA CATCACCTGG AGCCACAATT ACTCTGCAGG AACTGACTTT 
GTGGTTAAGT ATATCACCAG TAACAAGACA GAGAAATCTA TCCCCGTTAA AGCTCAGACC 
CCCTCCTCTG TGCAGCTGGC CAATCTGACG CCGGGGATGG TGTACAAGCT GTGGGTGTTC 
CCCATATGGT CTAGCCCCAG CGAGCACTCG TACATAACCT TTACCACCAG CTCAGCTTAC 
ACCAAGAACC ACGTGGACAT CGCGACCCAG GGCTGGTTCA TCGGGCTGAT GTGTGCCATC 
GCCCTCCTGG TCCTCATTCT GCTGATTGTT TGCTTCATTA AGAGGAGCAG AGGAGGGAAA 
TACCCAGTGC GTGACAACAA AGATGAGCAC CTGAATCCTG AAGACAAGAA CGTAGAAGAT 
GGCTCATTCG ACTACAGCGA TGAAGACAAC AAACCACTGC CCAACAGTCA GACCTCCCTG 
GATGGCACGA TAAAGCAACA GGAGAGTGAC GACAGCTTGG TGGACTACGG AGAGGGTGGG 
GAAGGGCAGT TCAACGAGGA CGGCTCCTTC ATTGGCCAGT ACACAGTGAA AAAGGACAAG 
GAGGAAACGG AAGGCAACGA GAGCTCAGAA GCCACGTCCC CAGTCAATGC TATCTACTCA 
TTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( XM_015298920.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154406.1 Gallus gallus neurofascin (NFASC), transcript variant X22, mRNA.

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CloneID	OGa46527
Clone ID Related Accession (Same CDS sequence)	XM_015298920.1 , XM_015298920.2
Accession Version	XM_015298920.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	neurofascin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298920.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA 
ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC 
CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG 
TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG 
GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC 
AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG 
CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA 
GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT 
TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG 
ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC 
AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC 
ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC 
CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG 
GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC 
ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA 
GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT 
CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG 
GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA 
TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC 
AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC 
TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG 
AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC 
GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT 
GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG 
ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC 
AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC 
AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT 
TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG 
GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG 
GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA 
GATTATCCCA AGGCTGCACC TACAGATGTT AGGATAAGAG TTTTAAACAG CACTGCCATT 
GCTCTGACAT GGACCCGCGT GCACCTGGAC ACCATCCAGG GACAGCTTAA GGAGTACAGA 
GCCTATTTCT GGAGAGACAG TAGTTTGCTG AAGAACCTGT GGGTCTCCAA AAAACGGCAG 
TATGTGAGTT TTCCTGGAGA CCGAAACCGG GGCATAGTGT CCCGGCTGTT CCCTTACAGC 
AACTACAAGC TTGAGATGGT TGTAACCAAC GGGAGAGGCG ATGGGCCCCG CAGCGAAGTC 
AAGGAGTTCC CCACACCTGA AGGAGTGCCC AGCTCCCCCA GGTACTTAAG AATCCGACAG 
CCAAATCTGG AAAGCATCAA TTTGGAGTGG GATCACCCAG AACATCCCAA TGGAGTCCTC 
ACGGGATACA ACCTTAGATA TCAAGCCTTT AACGGATCCA AAACGGGCAG AACCCTGGTA 
GAGAACTTCT CTCCCAACCA GACAAGGTTC ACCGTGCAGA GGACAGACCC CATCTCGCGC 
TATCGATTCT TCCTGCGTGC TCGGACACAG GTGGGAGATG GAGAAGTCAT AGTGGAAGAG 
TCACCAGCGC TCCTGAATGA AGCCACGCCA ACCCCAGCCT CCACCTGGTT GCCTCCACCT 
ACAACCGAGC TAACTCCAGC AGCCACCATT GCCACCACAA CCACCACTGC CACCCCTACT 
ACTGAAACCC CTCCTACTGA AATCCCTACT ACTGCCATCC CTACCACCAC CACTACTACA 
ACCACCACAG CTGCCAGCAC CGTCGCAAGC ACTACAACAA CTGCAGAGAG GGCTGCGGCA 
GCCACCACAA AACAGGAGCT GGCCACCAAT GGATCGTCCA TATGGGACAT AAGAGCTTGG 
GCTAATAGTA ACTGGGCAAA CATCACCTGG AGCCACAATT ACTCTGCAGG AACTGACTTT 
GTGGTTAAGT ATATCACCAG TAACAAGACA GAGAAATCTA TCCCCGTTAA AGCTCAGACC 
CCCTCCTCTG TGCAGCTGGC CAATCTGACG CCGGGGATGG TGTACAAGCT GTGGGTGTTC 
CCCATATGGT CTAGCCCCAG CGAGCACTCG TACATAACCT TTACCACCAG CTCAGCTTAC 
ACCAAGAACC ACGTGGACAT CGCGACCCAG GGCTGGTTCA TCGGGCTGAT GTGTGCCATC 
GCCCTCCTGG TCCTCATTCT GCTGATTGTT TGCTTCATTA AGAGGAGCAG AGGAGGGAAA 
TACCCAGTGC GTGACAACAA AGATGAGCAC CTGAATCCTG AAGACAAGAA CGTAGAAGAT 
GGCTCATTCG ACTACAGCGA TGAAGACAAC AAACCACTGC CCAACAGTCA GACCTCCCTG 
GATGGCACGA TAAAGCAACA GGAGAGTGAC GACAGCTTGG TGGACTACGG AGAGGGTGGG 
GAAGGGCAGT TCAACGAGGA CGGCTCCTTC ATTGGCCAGT ACACAGTGAA AAAGGACAAG 
GAGGAAACGG AAGGCAACGA GAGCTCAGAA GCCACGTCCC CAGTCAATGC TATCTACTCA 
TTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154406.1
CDS	169..4197
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTTRGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELATNGSSIWDIRAWANSNWANITWSHNYSAGTDFVVKYITSNKTEKSIPVKAQTPSSVQLANLTPGMVYKLWVFPIWSSPSEHSYITFTTSSAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYSDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_015298920.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298920.2

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA 
ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC 
CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG 
TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG 
GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC 
AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG 
CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA 
GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT 
TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG 
ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC 
AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC 
ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC 
CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG 
GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC 
ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA 
GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT 
CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG 
GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA 
TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC 
AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC 
TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG 
AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC 
GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT 
GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG 
ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC 
AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC 
AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT 
TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG 
GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG 
GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA 
GATTATCCCA AGGCTGCACC TACAGATGTT AGGATAAGAG TTTTAAACAG CACTGCCATT 
GCTCTGACAT GGACCCGCGT GCACCTGGAC ACCATCCAGG GACAGCTTAA GGAGTACAGA 
GCCTATTTCT GGAGAGACAG TAGTTTGCTG AAGAACCTGT GGGTCTCCAA AAAACGGCAG 
TATGTGAGTT TTCCTGGAGA CCGAAACCGG GGCATAGTGT CCCGGCTGTT CCCTTACAGC 
AACTACAAGC TTGAGATGGT TGTAACCAAC GGGAGAGGCG ATGGGCCCCG CAGCGAAGTC 
AAGGAGTTCC CCACACCTGA AGGAGTGCCC AGCTCCCCCA GGTACTTAAG AATCCGACAG 
CCAAATCTGG AAAGCATCAA TTTGGAGTGG GATCACCCAG AACATCCCAA TGGAGTCCTC 
ACGGGATACA ACCTTAGATA TCAAGCCTTT AACGGATCCA AAACGGGCAG AACCCTGGTA 
GAGAACTTCT CTCCCAACCA GACAAGGTTC ACCGTGCAGA GGACAGACCC CATCTCGCGC 
TATCGATTCT TCCTGCGTGC TCGGACACAG GTGGGAGATG GAGAAGTCAT AGTGGAAGAG 
TCACCAGCGC TCCTGAATGA AGCCACGCCA ACCCCAGCCT CCACCTGGTT GCCTCCACCT 
ACAACCGAGC TAACTCCAGC AGCCACCATT GCCACCACAA CCACCACTGC CACCCCTACT 
ACTGAAACCC CTCCTACTGA AATCCCTACT ACTGCCATCC CTACCACCAC CACTACTACA 
ACCACCACAG CTGCCAGCAC CGTCGCAAGC ACTACAACAA CTGCAGAGAG GGCTGCGGCA 
GCCACCACAA AACAGGAGCT GGCCACCAAT GGATCGTCCA TATGGGACAT AAGAGCTTGG 
GCTAATAGTA ACTGGGCAAA CATCACCTGG AGCCACAATT ACTCTGCAGG AACTGACTTT 
GTGGTTAAGT ATATCACCAG TAACAAGACA GAGAAATCTA TCCCCGTTAA AGCTCAGACC 
CCCTCCTCTG TGCAGCTGGC CAATCTGACG CCGGGGATGG TGTACAAGCT GTGGGTGTTC 
CCCATATGGT CTAGCCCCAG CGAGCACTCG TACATAACCT TTACCACCAG CTCAGCTTAC 
ACCAAGAACC ACGTGGACAT CGCGACCCAG GGCTGGTTCA TCGGGCTGAT GTGTGCCATC 
GCCCTCCTGG TCCTCATTCT GCTGATTGTT TGCTTCATTA AGAGGAGCAG AGGAGGGAAA 
TACCCAGTGC GTGACAACAA AGATGAGCAC CTGAATCCTG AAGACAAGAA CGTAGAAGAT 
GGCTCATTCG ACTACAGCGA TGAAGACAAC AAACCACTGC CCAACAGTCA GACCTCCCTG 
GATGGCACGA TAAAGCAACA GGAGAGTGAC GACAGCTTGG TGGACTACGG AGAGGGTGGG 
GAAGGGCAGT TCAACGAGGA CGGCTCCTTC ATTGGCCAGT ACACAGTGAA AAAGGACAAG 
GAGGAAACGG AAGGCAACGA GAGCTCAGAA GCCACGTCCC CAGTCAATGC TATCTACTCA 
TTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( NM_001160320.3 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001153792.1 Gallus gallus neurofascin (NFASC), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OGa28259
Clone ID Related Accession (Same CDS sequence)	NM_001160320.3
Accession Version	NM_001160320.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Gallus gallus(chicken)
Product	neurofascin isoform 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AADN05000168.1. On Feb 11, 2016 this sequence version replaced NM_001160320.2. Transcript Variant: This variant (4) lacks five alternate in-frame exons compared to variant 2. The resulting protein (isoform 4) lacks four internal segments, compared to isoform 2. This variant is the 2nd most abundant variant in E16 embryos and includes alternatively spliced exons 8, 22, and 23, as defined by Hassel et al (PMID: 9353344). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on data from Hassel et al (PMID: 9353344). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201358, SAMEA2201361 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG 
CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC 
AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA 
AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG 
GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC 
ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG 
AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT 
CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG 
GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG 
GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG 
AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA 
AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC 
CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC 
ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA 
GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG 
GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG 
ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG 
GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC 
CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG 
GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC 
AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG 
CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC 
CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC 
CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG 
AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC 
AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC 
GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC 
CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC 
AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT 
GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC 
GTGCTAGAGC GACCCGACCG GCCCCGGGAC CTGGAGCTGT CAGACCTGGC TGAGAGGAGC 
GTGAAGCTGA CATGGATTCC TGGCGATGAC AACAACAGCC CCATCACAGA CTACATCGTC 
CAGTTTGAGG AGGACCGCTT CCAGCCTGGC ACGTGGCACA ACCACTCCAG GTATCCTGGG 
AATGTCAACT CGGCCCTCCT GAGCCTCTCT CCTTACGTCA ACTACCAATT TCGGGTGATT 
GCAGTGAACG ACGTGGGCAG CAGCCTGCCC AGCATGCCCT CAGAACGATA CCAGACCAGC 
GGGGCACGAC CTGAAATTAA CCCAACAGGA GTTCAAGGAG CAGGGACCCA AAAAAACAAC 
ATGGAGATAA CCTGGACGCC TCTGAATGCA ACTCAAGCCT ATGGGCCCAA CCTCCGTTAC 
ATCGTGCGGT GGAGGCGAAG GGACCCACGT GGGAGTTGGT ACAACGAGAC GGTGAAGGCA 
CCACGACACG TCGTCTGGAA CACACCCATC TACGTCCCCT ACGAGATCAA AGTGCAGGCA 
GAGAATGACT TTGGTAGAGC TCCAGAGCCT GAGACCTACA TCGGCTACTC AGGGGAAGAT 
TATCCCAAGG CTGCACCTAC AGATGTTAGG ATAAGAGTTT TAAACAGCAC TGCCATTGCT 
CTGACATGGA CCCGCGTGCA CCTGGACACC ATCCAGGGAC AGCTTAAGGA GTACAGAGCC 
TATTTCTGGA GAGACAGTAG TTTGCTGAAG AACCTGTGGG TCTCCAAAAA ACGGCAGTAT 
GTGAGTTTTC CTGGAGACCG AAACCGGGGC ATAGTGTCCC GGCTGTTCCC TTACAGCAAC 
TACAAGCTTG AGATGGTTGT AACCAACGGG AGAGGCGATG GGCCCCGCAG CGAAGTCAAG 
GAGTTCCCCA CACCTGAAGG AGTGCCCAGC TCCCCCAGGT ACTTAAGAAT CCGACAGCCA 
AATCTGGAAA GCATCAATTT GGAGTGGGAT CACCCAGAAC ATCCCAATGG AGTCCTCACG 
GGATACAACC TTAGATATCA AGCCTTTAAC GGATCCAAAA CGGGCAGAAC CCTGGTAGAG 
AACTTCTCTC CCAACCAGAC AAGGTTCACC GTGCAGAGGA CAGACCCCAT CTCGCGCTAT 
CGATTCTTCC TGCGTGCTCG GACACAGGTG GGAGATGGAG AAGTCATAGT GGAAGAGTCA 
CCAGCGCTCC TGAATGAAGC TTACACCAAG AACCACGTGG ACATCGCGAC CCAGGGCTGG 
TTCATCGGGC TGATGTGTGC CATCGCCCTC CTGGTCCTCA TTCTGCTGAT TGTTTGCTTC 
ATTAAGAGGA GCAGAGGAGG GAAATACCCA GTGCGTGACA ACAAAGATGA GCACCTGAAT 
CCTGAAGACA AGAACGTAGA AGATGGCTCA TTCGACTACA GCGATGAAGA CAACAAACCA 
CTGCCCAACA GTCAGACCTC CCTGGATGGC ACGATAAAGC AACAGGAGAG TGACGACAGC 
TTGGTGGACT ACGGAGAGGG TGGGGAAGGG CAGTTCAACG AGGACGGCTC CTTCATTGGC 
CAGTACACAG TGAAAAAGGA CAAGGAGGAA ACGGAAGGCA ACGAGAGCTC AGAAGCCACG 
TCCCCAGTCA ATGCTATCTA CTCATTAGCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153792.1
CDS	109..3621
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDLPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYSDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	NM_001160320.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160320.3

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG 
CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC 
AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA 
AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG 
GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC 
ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG 
AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT 
CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG 
GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG 
GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG 
AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA 
AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC 
CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC 
ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA 
GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG 
GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG 
ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG 
GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC 
CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG 
GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC 
AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG 
CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC 
CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC 
CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG 
AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC 
AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC 
GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC 
CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC 
AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT 
GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC 
GTGCTAGAGC GACCCGACCG GCCCCGGGAC CTGGAGCTGT CAGACCTGGC TGAGAGGAGC 
GTGAAGCTGA CATGGATTCC TGGCGATGAC AACAACAGCC CCATCACAGA CTACATCGTC 
CAGTTTGAGG AGGACCGCTT CCAGCCTGGC ACGTGGCACA ACCACTCCAG GTATCCTGGG 
AATGTCAACT CGGCCCTCCT GAGCCTCTCT CCTTACGTCA ACTACCAATT TCGGGTGATT 
GCAGTGAACG ACGTGGGCAG CAGCCTGCCC AGCATGCCCT CAGAACGATA CCAGACCAGC 
GGGGCACGAC CTGAAATTAA CCCAACAGGA GTTCAAGGAG CAGGGACCCA AAAAAACAAC 
ATGGAGATAA CCTGGACGCC TCTGAATGCA ACTCAAGCCT ATGGGCCCAA CCTCCGTTAC 
ATCGTGCGGT GGAGGCGAAG GGACCCACGT GGGAGTTGGT ACAACGAGAC GGTGAAGGCA 
CCACGACACG TCGTCTGGAA CACACCCATC TACGTCCCCT ACGAGATCAA AGTGCAGGCA 
GAGAATGACT TTGGTAGAGC TCCAGAGCCT GAGACCTACA TCGGCTACTC AGGGGAAGAT 
TATCCCAAGG CTGCACCTAC AGATGTTAGG ATAAGAGTTT TAAACAGCAC TGCCATTGCT 
CTGACATGGA CCCGCGTGCA CCTGGACACC ATCCAGGGAC AGCTTAAGGA GTACAGAGCC 
TATTTCTGGA GAGACAGTAG TTTGCTGAAG AACCTGTGGG TCTCCAAAAA ACGGCAGTAT 
GTGAGTTTTC CTGGAGACCG AAACCGGGGC ATAGTGTCCC GGCTGTTCCC TTACAGCAAC 
TACAAGCTTG AGATGGTTGT AACCAACGGG AGAGGCGATG GGCCCCGCAG CGAAGTCAAG 
GAGTTCCCCA CACCTGAAGG AGTGCCCAGC TCCCCCAGGT ACTTAAGAAT CCGACAGCCA 
AATCTGGAAA GCATCAATTT GGAGTGGGAT CACCCAGAAC ATCCCAATGG AGTCCTCACG 
GGATACAACC TTAGATATCA AGCCTTTAAC GGATCCAAAA CGGGCAGAAC CCTGGTAGAG 
AACTTCTCTC CCAACCAGAC AAGGTTCACC GTGCAGAGGA CAGACCCCAT CTCGCGCTAT 
CGATTCTTCC TGCGTGCTCG GACACAGGTG GGAGATGGAG AAGTCATAGT GGAAGAGTCA 
CCAGCGCTCC TGAATGAAGC TTACACCAAG AACCACGTGG ACATCGCGAC CCAGGGCTGG 
TTCATCGGGC TGATGTGTGC CATCGCCCTC CTGGTCCTCA TTCTGCTGAT TGTTTGCTTC 
ATTAAGAGGA GCAGAGGAGG GAAATACCCA GTGCGTGACA ACAAAGATGA GCACCTGAAT 
CCTGAAGACA AGAACGTAGA AGATGGCTCA TTCGACTACA GCGATGAAGA CAACAAACCA 
CTGCCCAACA GTCAGACCTC CCTGGATGGC ACGATAAAGC AACAGGAGAG TGACGACAGC 
TTGGTGGACT ACGGAGAGGG TGGGGAAGGG CAGTTCAACG AGGACGGCTC CTTCATTGGC 
CAGTACACAG TGAAAAAGGA CAAGGAGGAA ACGGAAGGCA ACGAGAGCTC AGAAGCCACG 
TCCCCAGTCA ATGCTATCTA CTCATTAGCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( NM_001160322.3 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001153794.1 Gallus gallus neurofascin (NFASC), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OGa28267
Clone ID Related Accession (Same CDS sequence)	NM_001160322.3
Accession Version	NM_001160322.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Gallus gallus(chicken)
Product	neurofascin isoform 6 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AADN05000168.1. On Feb 11, 2016 this sequence version replaced NM_001160322.2. Transcript Variant: This variant (6) lacks four alternate in-frame exons and includes two alternate in-frame exons, compared to variant 2. The resulting protein (isoform 6) lacks three internal segments and includes an additional segment, compared to isoform 2. This variant is the 4th most abundant variant in E16 embryos and includes alternatively spliced exons 3, 17, 26, 27, 28, and 29, as defined by Hassel et al (PMID: 9353344). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on data from Hassel et al (PMID: 9353344). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201358, SAMEA2201363 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA 
ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC 
CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG 
TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG 
GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC 
AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG 
CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA 
GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT 
TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG 
ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC 
AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC 
ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC 
CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG 
GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC 
ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA 
GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT 
CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG 
GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA 
TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC 
AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC 
TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG 
AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC 
GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT 
GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG 
ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC 
AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC 
AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT 
TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG 
GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG 
GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA 
GATTTGCCCA GCTCCCCCAG GTACTTAAGA ATCCGACAGC CAAATCTGGA AAGCATCAAT 
TTGGAGTGGG ATCACCCAGA ACATCCCAAT GGAGTCCTCA CGGGATACAA CCTTAGATAT 
CAAGCCTTTA ACGGATCCAA AACGGGCAGA ACCCTGGTAG AGAACTTCTC TCCCAACCAG 
ACAAGGTTCA CCGTGCAGAG GACAGACCCC ATCTCGCGCT ATCGATTCTT CCTGCGTGCT 
CGGACACAGG TGGGAGATGG AGAAGTCATA GTGGAAGAGT CACCAGCGCT CCTGAATGAA 
GCCACGCCAA CCCCAGCCTC CACCTGGTTG CCTCCACCTA CAACCGAGCT AACTCCAGCA 
GCCACCATTG CCACCACAAC CACCACTGCC ACCCCTACTA CTGAAACCCC TCCTACTGAA 
ATCCCTACTA CTGCCATCCC TACCACCACC ACTACTACAA CCACCACAGC TGCCAGCACC 
GTCGCAAGCA CTACAACAAC TGCAGAGAGG GCTGCGGCAG CCACCACAAA ACAGGAGCTG 
GCCACCAATG GATCGTCCAT ATGGGACATA AGAGCTTGGG CTAATAGTAA CTGGGCAAAC 
ATCACCTGGA GCCACAATTA CTCTGCAGGA ACTGACTTTG TGGTTAAGTA TATCACCAGT 
AACAAGACAG AGAAATCTAT CCCCGTTAAA GCTCAGACCC CCTCCTCTGT GCAGCTGGCC 
AATCTGACGC CGGGGATGGT GTACAAGCTG TGGGTGTTCC CCATATGGTC TAGCCCCAGC 
GAGCACTCGT ACATAACCTT TACCACCAGC TCAGCTTACA CCAAGAACCA CGTGGACATC 
GCGACCCAGG GCTGGTTCAT CGGGCTGATG TGTGCCATCG CCCTCCTGGT CCTCATTCTG 
CTGATTGTTT GCTTCATTAA GAGGAGCAGA GGAGGGAAAT ACCCAGTGCG TGACAACAAA 
GATGAGCACC TGAATCCTGA AGACAAGAAC GTAGAAGATG GCTCATTCGA CTACAGCGAT 
GAAGACAACA AACCACTGCC CAACAGTCAG ACCTCCCTGG ATGGCACGAT AAAGCAACAG 
GAGAGTGACG ACAGCTTGGT GGACTACGGA GAGGGTGGGG AAGGGCAGTT CAACGAGGAC 
GGCTCCTTCA TTGGCCAGTA CACAGTGAAA AAGGACAAGG AGGAAACGGA AGGCAACGAG 
AGCTCAGAAG CCACGTCCCC AGTCAATGCT ATCTACTCAT TAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153794.1
CDS	109..3816
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTTRGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDLPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELATNGSSIWDIRAWANSNWANITWSHNYSAGTDFVVKYITSNKTEKSIPVKAQTPSSVQLANLTPGMVYKLWVFPIWSSPSEHSYITFTTSSAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYSDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	NM_001160322.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160322.3

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA 
ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC 
CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG 
TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG 
GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC 
AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG 
CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA 
GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT 
TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG 
ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC 
AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC 
ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC 
CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG 
GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC 
ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA 
GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT 
CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG 
GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA 
TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC 
AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC 
TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG 
AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC 
GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT 
GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG 
ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC 
AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC 
AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT 
TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG 
GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG 
GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA 
GATTTGCCCA GCTCCCCCAG GTACTTAAGA ATCCGACAGC CAAATCTGGA AAGCATCAAT 
TTGGAGTGGG ATCACCCAGA ACATCCCAAT GGAGTCCTCA CGGGATACAA CCTTAGATAT 
CAAGCCTTTA ACGGATCCAA AACGGGCAGA ACCCTGGTAG AGAACTTCTC TCCCAACCAG 
ACAAGGTTCA CCGTGCAGAG GACAGACCCC ATCTCGCGCT ATCGATTCTT CCTGCGTGCT 
CGGACACAGG TGGGAGATGG AGAAGTCATA GTGGAAGAGT CACCAGCGCT CCTGAATGAA 
GCCACGCCAA CCCCAGCCTC CACCTGGTTG CCTCCACCTA CAACCGAGCT AACTCCAGCA 
GCCACCATTG CCACCACAAC CACCACTGCC ACCCCTACTA CTGAAACCCC TCCTACTGAA 
ATCCCTACTA CTGCCATCCC TACCACCACC ACTACTACAA CCACCACAGC TGCCAGCACC 
GTCGCAAGCA CTACAACAAC TGCAGAGAGG GCTGCGGCAG CCACCACAAA ACAGGAGCTG 
GCCACCAATG GATCGTCCAT ATGGGACATA AGAGCTTGGG CTAATAGTAA CTGGGCAAAC 
ATCACCTGGA GCCACAATTA CTCTGCAGGA ACTGACTTTG TGGTTAAGTA TATCACCAGT 
AACAAGACAG AGAAATCTAT CCCCGTTAAA GCTCAGACCC CCTCCTCTGT GCAGCTGGCC 
AATCTGACGC CGGGGATGGT GTACAAGCTG TGGGTGTTCC CCATATGGTC TAGCCCCAGC 
GAGCACTCGT ACATAACCTT TACCACCAGC TCAGCTTACA CCAAGAACCA CGTGGACATC 
GCGACCCAGG GCTGGTTCAT CGGGCTGATG TGTGCCATCG CCCTCCTGGT CCTCATTCTG 
CTGATTGTTT GCTTCATTAA GAGGAGCAGA GGAGGGAAAT ACCCAGTGCG TGACAACAAA 
GATGAGCACC TGAATCCTGA AGACAAGAAC GTAGAAGATG GCTCATTCGA CTACAGCGAT 
GAAGACAACA AACCACTGCC CAACAGTCAG ACCTCCCTGG ATGGCACGAT AAAGCAACAG 
GAGAGTGACG ACAGCTTGGT GGACTACGGA GAGGGTGGGG AAGGGCAGTT CAACGAGGAC 
GGCTCCTTCA TTGGCCAGTA CACAGTGAAA AAGGACAAGG AGGAAACGGA AGGCAACGAG 
AGCTCAGAAG CCACGTCCCC AGTCAATGCT ATCTACTCAT TAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( NM_001004493.4 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001004493.1 Gallus gallus neurofascin (NFASC), transcript variant 2, mRNA.

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CloneID	OGa28270
Clone ID Related Accession (Same CDS sequence)	NM_001004493.4
Accession Version	NM_001004493.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Gallus gallus(chicken)
Product	neurofascin isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AADN05000168.1. On Feb 11, 2016 this sequence version replaced NM_001004493.3. Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: X65224.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201358, SAMEA2201363 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG 
CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC 
CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA 
GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA 
AAACAGGAGC TGGCTTACAC CAAGAACCAC GTGGACATCG CGACCCAGGG CTGGTTCATC 
GGGCTGATGT GTGCCATCGC CCTCCTGGTC CTCATTCTGC TGATTGTTTG CTTCATTAAG 
AGGAGCAGAG GAGGGAAATA CCCAGTGCGT GACAACAAAG ATGAGCACCT GAATCCTGAA 
GACAAGAACG TAGAAGATGG CTCATTCGAC TACAGGTCTC TTGAAAGCGA TGAAGACAAC 
AAACCACTGC CCAACAGTCA GACCTCCCTG GATGGCACGA TAAAGCAACA GGAGAGTGAC 
GACAGCTTGG TGGACTACGG AGAGGGTGGG GAAGGGCAGT TCAACGAGGA CGGCTCCTTC 
ATTGGCCAGT ACACAGTGAA AAAGGACAAG GAGGAAACGG AAGGCAACGA GAGCTCAGAA 
GCCACGTCCC CAGTCAATGC TATCTACTCA TTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004493.1
CDS	109..3927
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	NM_001004493.4
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004493.4

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT 
AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA 
ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG 
GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG 
AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT 
ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG 
GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG 
CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG 
AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC 
AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC 
AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT 
GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC 
ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG 
CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG 
AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC 
GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC 
AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC 
TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT 
CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG 
GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA 
GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA 
GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG 
CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT 
GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC 
AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG 
AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA 
CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG 
ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG 
CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA 
CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT 
GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC 
AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA 
TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC 
TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT 
TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG 
CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC 
CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG 
GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC 
AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC 
TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC 
TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG 
CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG 
CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC 
CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA 
GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA 
AAACAGGAGC TGGCTTACAC CAAGAACCAC GTGGACATCG CGACCCAGGG CTGGTTCATC 
GGGCTGATGT GTGCCATCGC CCTCCTGGTC CTCATTCTGC TGATTGTTTG CTTCATTAAG 
AGGAGCAGAG GAGGGAAATA CCCAGTGCGT GACAACAAAG ATGAGCACCT GAATCCTGAA 
GACAAGAACG TAGAAGATGG CTCATTCGAC TACAGGTCTC TTGAAAGCGA TGAAGACAAC 
AAACCACTGC CCAACAGTCA GACCTCCCTG GATGGCACGA TAAAGCAACA GGAGAGTGAC 
GACAGCTTGG TGGACTACGG AGAGGGTGGG GAAGGGCAGT TCAACGAGGA CGGCTCCTTC 
ATTGGCCAGT ACACAGTGAA AAAGGACAAG GAGGAAACGG AAGGCAACGA GAGCTCAGAA 
GCCACGTCCC CAGTCAATGC TATCTACTCA TTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( NM_001160321.3 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001153793.1 Gallus gallus neurofascin (NFASC), transcript variant 5, mRNA.

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CloneID	OGa28275
Clone ID Related Accession (Same CDS sequence)	NM_001160321.3
Accession Version	NM_001160321.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Gallus gallus(chicken)
Product	neurofascin isoform 5 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AADN05000168.1. On Feb 11, 2016 this sequence version replaced NM_001160321.2. Transcript Variant: This variant (5) lacks five alternate in-frame exons compared to variant 2. The resulting protein (isoform 5) lacks four internal segments, compared to isoform 2. This variant is the 3rd most abundant variant in E16 embryos and includes alternatively spliced exons 3, 17, and 26, as defined by Hassel et al (PMID: 9353344). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on data from Hassel et al (PMID: 9353344). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201358, SAMEA2201363 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA 
ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC 
CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG 
TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG 
GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC 
AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG 
CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA 
GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT 
TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG 
ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC 
AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC 
ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC 
CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG 
GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC 
ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA 
GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT 
CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG 
GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA 
TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC 
AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC 
TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG 
AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC 
GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT 
GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG 
ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC 
AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC 
AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT 
TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG 
GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG 
GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA 
GATTTGCCCA GCTCCCCCAG GTACTTAAGA ATCCGACAGC CAAATCTGGA AAGCATCAAT 
TTGGAGTGGG ATCACCCAGA ACATCCCAAT GGAGTCCTCA CGGGATACAA CCTTAGATAT 
CAAGCCTTTA ACGGATCCAA AACGGGCAGA ACCCTGGTAG AGAACTTCTC TCCCAACCAG 
ACAAGGTTCA CCGTGCAGAG GACAGACCCC ATCTCGCGCT ATCGATTCTT CCTGCGTGCT 
CGGACACAGG TGGGAGATGG AGAAGTCATA GTGGAAGAGT CACCAGCGCT CCTGAATGAA 
GCCACGCCAA CCCCAGCTTA CACCAAGAAC CACGTGGACA TCGCGACCCA GGGCTGGTTC 
ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG GTCCTCATTC TGCTGATTGT TTGCTTCATT 
AAGAGGAGCA GAGGAGGGAA ATACCCAGTG CGTGACAACA AAGATGAGCA CCTGAATCCT 
GAAGACAAGA ACGTAGAAGA TGGCTCATTC GACTACAGCG ATGAAGACAA CAAACCACTG 
CCCAACAGTC AGACCTCCCT GGATGGCACG ATAAAGCAAC AGGAGAGTGA CGACAGCTTG 
GTGGACTACG GAGAGGGTGG GGAAGGGCAG TTCAACGAGG ACGGCTCCTT CATTGGCCAG 
TACACAGTGA AAAAGGACAA GGAGGAAACG GAAGGCAACG AGAGCTCAGA AGCCACGTCC 
CCAGTCAATG CTATCTACTC ATTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153793.1
CDS	109..3318
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTTRGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDLPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYSDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	NM_001160321.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160321.3

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA 
ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC 
CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG 
TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG 
GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC 
AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG 
CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA 
GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT 
TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG 
ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC 
AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC 
ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC 
CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG 
GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC 
ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA 
GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT 
CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG 
GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA 
TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC 
AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC 
TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG 
AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC 
GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT 
GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG 
ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC 
AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC 
AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT 
TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG 
GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG 
GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA 
GATTTGCCCA GCTCCCCCAG GTACTTAAGA ATCCGACAGC CAAATCTGGA AAGCATCAAT 
TTGGAGTGGG ATCACCCAGA ACATCCCAAT GGAGTCCTCA CGGGATACAA CCTTAGATAT 
CAAGCCTTTA ACGGATCCAA AACGGGCAGA ACCCTGGTAG AGAACTTCTC TCCCAACCAG 
ACAAGGTTCA CCGTGCAGAG GACAGACCCC ATCTCGCGCT ATCGATTCTT CCTGCGTGCT 
CGGACACAGG TGGGAGATGG AGAAGTCATA GTGGAAGAGT CACCAGCGCT CCTGAATGAA 
GCCACGCCAA CCCCAGCTTA CACCAAGAAC CACGTGGACA TCGCGACCCA GGGCTGGTTC 
ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG GTCCTCATTC TGCTGATTGT TTGCTTCATT 
AAGAGGAGCA GAGGAGGGAA ATACCCAGTG CGTGACAACA AAGATGAGCA CCTGAATCCT 
GAAGACAAGA ACGTAGAAGA TGGCTCATTC GACTACAGCG ATGAAGACAA CAAACCACTG 
CCCAACAGTC AGACCTCCCT GGATGGCACG ATAAAGCAAC AGGAGAGTGA CGACAGCTTG 
GTGGACTACG GAGAGGGTGG GGAAGGGCAG TTCAACGAGG ACGGCTCCTT CATTGGCCAG 
TACACAGTGA AAAAGGACAA GGAGGAAACG GAAGGCAACG AGAGCTCAGA AGCCACGTCC 
CCAGTCAATG CTATCTACTC ATTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( NM_001160323.3 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001153795.1 Gallus gallus neurofascin (NFASC), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OGa28276
Clone ID Related Accession (Same CDS sequence)	NM_001160323.3
Accession Version	NM_001160323.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Gallus gallus(chicken)
Product	neurofascin isoform 7 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AADN05000168.1. On Feb 11, 2016 this sequence version replaced NM_001160323.2. Transcript Variant: This variant (7) lacks three alternate in-frame exons compared to variant 2. The resulting protein (isoform 7) lacks three internal segments, compared to isoform 2. This variant is the 5th most abundant variant in E16 embryos and includes alternatively spliced exons 8, 22, 23, 26, and 32, as defined by Hassel et al (PMID: 9353344). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on data from Hassel et al (PMID: 9353344). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201358, SAMEA2201361 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG 
CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC 
AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA 
AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG 
GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC 
ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG 
AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT 
CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG 
GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG 
GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG 
AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA 
AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC 
CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC 
ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA 
GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG 
GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG 
ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG 
GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC 
CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG 
GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC 
AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG 
CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC 
CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC 
CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG 
AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC 
AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC 
GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC 
CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC 
AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT 
GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC 
GTGCTAGAGC GACCCGACCG GCCCCGGGAC CTGGAGCTGT CAGACCTGGC TGAGAGGAGC 
GTGAAGCTGA CATGGATTCC TGGCGATGAC AACAACAGCC CCATCACAGA CTACATCGTC 
CAGTTTGAGG AGGACCGCTT CCAGCCTGGC ACGTGGCACA ACCACTCCAG GTATCCTGGG 
AATGTCAACT CGGCCCTCCT GAGCCTCTCT CCTTACGTCA ACTACCAATT TCGGGTGATT 
GCAGTGAACG ACGTGGGCAG CAGCCTGCCC AGCATGCCCT CAGAACGATA CCAGACCAGC 
GGGGCACGAC CTGAAATTAA CCCAACAGGA GTTCAAGGAG CAGGGACCCA AAAAAACAAC 
ATGGAGATAA CCTGGACGCC TCTGAATGCA ACTCAAGCCT ATGGGCCCAA CCTCCGTTAC 
ATCGTGCGGT GGAGGCGAAG GGACCCACGT GGGAGTTGGT ACAACGAGAC GGTGAAGGCA 
CCACGACACG TCGTCTGGAA CACACCCATC TACGTCCCCT ACGAGATCAA AGTGCAGGCA 
GAGAATGACT TTGGTAGAGC TCCAGAGCCT GAGACCTACA TCGGCTACTC AGGGGAAGAT 
TATCCCAAGG CTGCACCTAC AGATGTTAGG ATAAGAGTTT TAAACAGCAC TGCCATTGCT 
CTGACATGGA CCCGCGTGCA CCTGGACACC ATCCAGGGAC AGCTTAAGGA GTACAGAGCC 
TATTTCTGGA GAGACAGTAG TTTGCTGAAG AACCTGTGGG TCTCCAAAAA ACGGCAGTAT 
GTGAGTTTTC CTGGAGACCG AAACCGGGGC ATAGTGTCCC GGCTGTTCCC TTACAGCAAC 
TACAAGCTTG AGATGGTTGT AACCAACGGG AGAGGCGATG GGCCCCGCAG CGAAGTCAAG 
GAGTTCCCCA CACCTGAAGG AGTGCCCAGC TCCCCCAGGT ACTTAAGAAT CCGACAGCCA 
AATCTGGAAA GCATCAATTT GGAGTGGGAT CACCCAGAAC ATCCCAATGG AGTCCTCACG 
GGATACAACC TTAGATATCA AGCCTTTAAC GGATCCAAAA CGGGCAGAAC CCTGGTAGAG 
AACTTCTCTC CCAACCAGAC AAGGTTCACC GTGCAGAGGA CAGACCCCAT CTCGCGCTAT 
CGATTCTTCC TGCGTGCTCG GACACAGGTG GGAGATGGAG AAGTCATAGT GGAAGAGTCA 
CCAGCGCTCC TGAATGAAGC CACGCCAACC CCAGCTTACA CCAAGAACCA CGTGGACATC 
GCGACCCAGG GCTGGTTCAT CGGGCTGATG TGTGCCATCG CCCTCCTGGT CCTCATTCTG 
CTGATTGTTT GCTTCATTAA GAGGAGCAGA GGAGGGAAAT ACCCAGTGCG TGACAACAAA 
GATGAGCACC TGAATCCTGA AGACAAGAAC GTAGAAGATG GCTCATTCGA CTACAGGTCT 
CTTGAAAGCG ATGAAGACAA CAAACCACTG CCCAACAGTC AGACCTCCCT GGATGGCACG 
ATAAAGCAAC AGGAGAGTGA CGACAGCTTG GTGGACTACG GAGAGGGTGG GGAAGGGCAG 
TTCAACGAGG ACGGCTCCTT CATTGGCCAG TACACAGTGA AAAAGGACAA GGAGGAAACG 
GAAGGCAACG AGAGCTCAGA AGCCACGTCC CCAGTCAATG CTATCTACTC ATTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153795.1
CDS	109..3648
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDLPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTKKPHNETSLRNHTDMYSARGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDYPKAAPTDVRIRVLNSTAIALTWTRVHLDTIQGQLKEYRAYFWRDSSLLKNLWVSKKRQYVSFPGDRNRGIVSRLFPYSNYKLEMVVTNGRGDGPRSEVKEFPTPEGVPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYRSLESDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	NM_001160323.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160323.3

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG 
CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC 
AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA 
AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG 
GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC 
ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG 
AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT 
CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG 
GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG 
GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG 
AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA 
AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC 
CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC 
ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA 
GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG 
GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG 
ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG 
GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC 
CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG 
GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC 
AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG 
CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC 
CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC 
CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG 
AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC 
AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC 
GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC 
CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC 
AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT 
GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC 
GTGCTAGAGC GACCCGACCG GCCCCGGGAC CTGGAGCTGT CAGACCTGGC TGAGAGGAGC 
GTGAAGCTGA CATGGATTCC TGGCGATGAC AACAACAGCC CCATCACAGA CTACATCGTC 
CAGTTTGAGG AGGACCGCTT CCAGCCTGGC ACGTGGCACA ACCACTCCAG GTATCCTGGG 
AATGTCAACT CGGCCCTCCT GAGCCTCTCT CCTTACGTCA ACTACCAATT TCGGGTGATT 
GCAGTGAACG ACGTGGGCAG CAGCCTGCCC AGCATGCCCT CAGAACGATA CCAGACCAGC 
GGGGCACGAC CTGAAATTAA CCCAACAGGA GTTCAAGGAG CAGGGACCCA AAAAAACAAC 
ATGGAGATAA CCTGGACGCC TCTGAATGCA ACTCAAGCCT ATGGGCCCAA CCTCCGTTAC 
ATCGTGCGGT GGAGGCGAAG GGACCCACGT GGGAGTTGGT ACAACGAGAC GGTGAAGGCA 
CCACGACACG TCGTCTGGAA CACACCCATC TACGTCCCCT ACGAGATCAA AGTGCAGGCA 
GAGAATGACT TTGGTAGAGC TCCAGAGCCT GAGACCTACA TCGGCTACTC AGGGGAAGAT 
TATCCCAAGG CTGCACCTAC AGATGTTAGG ATAAGAGTTT TAAACAGCAC TGCCATTGCT 
CTGACATGGA CCCGCGTGCA CCTGGACACC ATCCAGGGAC AGCTTAAGGA GTACAGAGCC 
TATTTCTGGA GAGACAGTAG TTTGCTGAAG AACCTGTGGG TCTCCAAAAA ACGGCAGTAT 
GTGAGTTTTC CTGGAGACCG AAACCGGGGC ATAGTGTCCC GGCTGTTCCC TTACAGCAAC 
TACAAGCTTG AGATGGTTGT AACCAACGGG AGAGGCGATG GGCCCCGCAG CGAAGTCAAG 
GAGTTCCCCA CACCTGAAGG AGTGCCCAGC TCCCCCAGGT ACTTAAGAAT CCGACAGCCA 
AATCTGGAAA GCATCAATTT GGAGTGGGAT CACCCAGAAC ATCCCAATGG AGTCCTCACG 
GGATACAACC TTAGATATCA AGCCTTTAAC GGATCCAAAA CGGGCAGAAC CCTGGTAGAG 
AACTTCTCTC CCAACCAGAC AAGGTTCACC GTGCAGAGGA CAGACCCCAT CTCGCGCTAT 
CGATTCTTCC TGCGTGCTCG GACACAGGTG GGAGATGGAG AAGTCATAGT GGAAGAGTCA 
CCAGCGCTCC TGAATGAAGC CACGCCAACC CCAGCTTACA CCAAGAACCA CGTGGACATC 
GCGACCCAGG GCTGGTTCAT CGGGCTGATG TGTGCCATCG CCCTCCTGGT CCTCATTCTG 
CTGATTGTTT GCTTCATTAA GAGGAGCAGA GGAGGGAAAT ACCCAGTGCG TGACAACAAA 
GATGAGCACC TGAATCCTGA AGACAAGAAC GTAGAAGATG GCTCATTCGA CTACAGGTCT 
CTTGAAAGCG ATGAAGACAA CAAACCACTG CCCAACAGTC AGACCTCCCT GGATGGCACG 
ATAAAGCAAC AGGAGAGTGA CGACAGCTTG GTGGACTACG GAGAGGGTGG GGAAGGGCAG 
TTCAACGAGG ACGGCTCCTT CATTGGCCAG TACACAGTGA AAAAGGACAA GGAGGAAACG 
GAAGGCAACG AGAGCTCAGA AGCCACGTCC CCAGTCAATG CTATCTACTC ATTAGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( NM_001160324.3 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001153796.1 Gallus gallus neurofascin (NFASC), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OGa28282
Clone ID Related Accession (Same CDS sequence)	NM_001160324.3
Accession Version	NM_001160324.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Gallus gallus(chicken)
Product	neurofascin isoform 8 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AADN05000168.1. On Feb 10, 2016 this sequence version replaced NM_001160324.2. Transcript Variant: This variant (8) lacks six alternate in-frame exons compared to variant 2. The resulting protein (isoform 8) lacks four internal segments, compared to isoform 2. This variant is the most abundant variant in E06 embryos and includes alternatively spliced exons 3 and 17, as defined by Hassel et al (PMID: 9353344). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on data from Hassel et al (PMID: 9353344). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201357, SAMEA2201358 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA 
ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC 
CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG 
TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG 
GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC 
AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG 
CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA 
GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT 
TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG 
ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC 
AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC 
ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC 
CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG 
GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC 
ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA 
GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT 
CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG 
GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA 
TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC 
AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC 
TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG 
AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC 
GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT 
GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG 
ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC 
AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC 
AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT 
TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG 
GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG 
GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA 
GATTTGCCCA GCTCCCCCAG GTACTTAAGA ATCCGACAGC CAAATCTGGA AAGCATCAAT 
TTGGAGTGGG ATCACCCAGA ACATCCCAAT GGAGTCCTCA CGGGATACAA CCTTAGATAT 
CAAGCCTTTA ACGGATCCAA AACGGGCAGA ACCCTGGTAG AGAACTTCTC TCCCAACCAG 
ACAAGGTTCA CCGTGCAGAG GACAGACCCC ATCTCGCGCT ATCGATTCTT CCTGCGTGCT 
CGGACACAGG TGGGAGATGG AGAAGTCATA GTGGAAGAGT CACCAGCGCT CCTGAATGAA 
GCTTACACCA AGAACCACGT GGACATCGCG ACCCAGGGCT GGTTCATCGG GCTGATGTGT 
GCCATCGCCC TCCTGGTCCT CATTCTGCTG ATTGTTTGCT TCATTAAGAG GAGCAGAGGA 
GGGAAATACC CAGTGCGTGA CAACAAAGAT GAGCACCTGA ATCCTGAAGA CAAGAACGTA 
GAAGATGGCT CATTCGACTA CAGCGATGAA GACAACAAAC CACTGCCCAA CAGTCAGACC 
TCCCTGGATG GCACGATAAA GCAACAGGAG AGTGACGACA GCTTGGTGGA CTACGGAGAG 
GGTGGGGAAG GGCAGTTCAA CGAGGACGGC TCCTTCATTG GCCAGTACAC AGTGAAAAAG 
GACAAGGAGG AAACGGAAGG CAACGAGAGC TCAGAAGCCA CGTCCCCAGT CAATGCTATC 
TACTCATTAG CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153796.1
CDS	109..3303
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTTRGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDLPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYSDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	NM_001160324.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160324.3

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA 
ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC 
CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG 
TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG 
GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC 
AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG 
CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA 
GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT 
TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG 
ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC 
AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC 
ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC 
CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG 
GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC 
ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA 
GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT 
CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG 
GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA 
TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC 
AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC 
TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG 
AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC 
GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT 
GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG 
ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC 
AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC 
AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT 
TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG 
GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG 
GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA 
GATTTGCCCA GCTCCCCCAG GTACTTAAGA ATCCGACAGC CAAATCTGGA AAGCATCAAT 
TTGGAGTGGG ATCACCCAGA ACATCCCAAT GGAGTCCTCA CGGGATACAA CCTTAGATAT 
CAAGCCTTTA ACGGATCCAA AACGGGCAGA ACCCTGGTAG AGAACTTCTC TCCCAACCAG 
ACAAGGTTCA CCGTGCAGAG GACAGACCCC ATCTCGCGCT ATCGATTCTT CCTGCGTGCT 
CGGACACAGG TGGGAGATGG AGAAGTCATA GTGGAAGAGT CACCAGCGCT CCTGAATGAA 
GCTTACACCA AGAACCACGT GGACATCGCG ACCCAGGGCT GGTTCATCGG GCTGATGTGT 
GCCATCGCCC TCCTGGTCCT CATTCTGCTG ATTGTTTGCT TCATTAAGAG GAGCAGAGGA 
GGGAAATACC CAGTGCGTGA CAACAAAGAT GAGCACCTGA ATCCTGAAGA CAAGAACGTA 
GAAGATGGCT CATTCGACTA CAGCGATGAA GACAACAAAC CACTGCCCAA CAGTCAGACC 
TCCCTGGATG GCACGATAAA GCAACAGGAG AGTGACGACA GCTTGGTGGA CTACGGAGAG 
GGTGGGGAAG GGCAGTTCAA CGAGGACGGC TCCTTCATTG GCCAGTACAC AGTGAAAAAG 
GACAAGGAGG AAACGGAAGG CAACGAGAGC TCAGAAGCCA CGTCCCCAGT CAATGCTATC 
TACTCATTAG CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFASC ( NM_001004709.4 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001004709.2 Gallus gallus neurofascin (NFASC), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OGa28289
Clone ID Related Accession (Same CDS sequence)	NM_001004709.4
Accession Version	NM_001004709.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Gallus gallus(chicken)
Product	neurofascin isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AADN05000168.1. On Feb 11, 2016 this sequence version replaced NM_001004709.3. Transcript Variant: This variant (3) lacks four alternate in-frame exons compared to variant 2. The resulting protein (isoform 3) lacks three internal segments, compared to isoform 2. This variant is the most abundant variant in E16 embryos and includes alternatively spliced exons 3, 17, 26, and 27, as defined by Hassel et al (PMID: 9353344). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on data from Hassel et al (PMID: 9353344). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201358, SAMEA2201363 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA 
ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC 
CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG 
TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG 
GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC 
AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG 
CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA 
GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT 
TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG 
ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC 
AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC 
ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC 
CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG 
GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC 
ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA 
GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT 
CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG 
GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA 
TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC 
AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC 
TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG 
AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC 
GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT 
GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG 
ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC 
AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC 
AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT 
TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG 
GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG 
GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA 
GATTTGCCCA GCTCCCCCAG GTACTTAAGA ATCCGACAGC CAAATCTGGA AAGCATCAAT 
TTGGAGTGGG ATCACCCAGA ACATCCCAAT GGAGTCCTCA CGGGATACAA CCTTAGATAT 
CAAGCCTTTA ACGGATCCAA AACGGGCAGA ACCCTGGTAG AGAACTTCTC TCCCAACCAG 
ACAAGGTTCA CCGTGCAGAG GACAGACCCC ATCTCGCGCT ATCGATTCTT CCTGCGTGCT 
CGGACACAGG TGGGAGATGG AGAAGTCATA GTGGAAGAGT CACCAGCGCT CCTGAATGAA 
GCCACGCCAA CCCCAGCCTC CACCTGGTTG CCTCCACCTA CAACCGAGCT AACTCCAGCA 
GCCACCATTG CCACCACAAC CACCACTGCC ACCCCTACTA CTGAAACCCC TCCTACTGAA 
ATCCCTACTA CTGCCATCCC TACCACCACC ACTACTACAA CCACCACAGC TGCCAGCACC 
GTCGCAAGCA CTACAACAAC TGCAGAGAGG GCTGCGGCAG CCACCACAAA ACAGGAGCTG 
GCTTACACCA AGAACCACGT GGACATCGCG ACCCAGGGCT GGTTCATCGG GCTGATGTGT 
GCCATCGCCC TCCTGGTCCT CATTCTGCTG ATTGTTTGCT TCATTAAGAG GAGCAGAGGA 
GGGAAATACC CAGTGCGTGA CAACAAAGAT GAGCACCTGA ATCCTGAAGA CAAGAACGTA 
GAAGATGGCT CATTCGACTA CAGCGATGAA GACAACAAAC CACTGCCCAA CAGTCAGACC 
TCCCTGGATG GCACGATAAA GCAACAGGAG AGTGACGACA GCTTGGTGGA CTACGGAGAG 
GGTGGGGAAG GGCAGTTCAA CGAGGACGGC TCCTTCATTG GCCAGTACAC AGTGAAAAAG 
GACAAGGAGG AAACGGAAGG CAACGAGAGC TCAGAAGCCA CGTCCCCAGT CAATGCTATC 
TACTCATTAG CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004709.2
CDS	109..3543
Translation	MVLHSHQLTYAGIAFALCLHHLISAIEVPLDSNIQSELPQPPTITKQSVKDYIVDPRDNIFIECEAKGNPVPTFSWTRNGKFFNVAKDPKVSMRRRSGTLVIDFHGGGRPDDYEGEYQCFARNDYGTALSSKIHLQVSRSPLWPKEKVDVIEVDEGAPLSLQCNPPPGLPPPVIFWMSSSMEPIHQDKRVSQGQNGDLYFSNVMLQDAQTDYSCNARFHFTHTIQQKNPYTLKVKTTRGVTETTPSFMYPYGTSSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPQNLILAPGEDGRLVCRANGNPKPSIQWLVNGEPIEGSPPNPSREVAGDTIVFRDTQIGSSAVYQCNASNEHGYLLANAFVSVLDVPPRILAPRNQLIKVIQYNRTRLDCPFFGSPIPTLRWFKNGQGNMLDGGNYKAHENGSLEMSMARKEDQGIYTCVATNILGKVEAQVRLEVKDPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLAIPANRLRDLPKERPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSERYQTSGARPEINPTGVQGAGTQKNNMEITWTPLNATQAYGPNLRYIVRWRRRDPRGSWYNETVKAPRHVVWNTPIYVPYEIKVQAENDFGRAPEPETYIGYSGEDLPSSPRYLRIRQPNLESINLEWDHPEHPNGVLTGYNLRYQAFNGSKTGRTLVENFSPNQTRFTVQRTDPISRYRFFLRARTQVGDGEVIVEESPALLNEATPTPASTWLPPPTTELTPAATIATTTTTATPTTETPPTEIPTTAIPTTTTTTTTTAASTVASTTTTAERAAAATTKQELAYTKNHVDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVRDNKDEHLNPEDKNVEDGSFDYSDEDNKPLPNSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	NM_001004709.4
Organism	Gallus gallus(chicken)
Definition	Gallus gallus neurofascin (NFASC), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004709.4

ORF Insert Sequence:

ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG 
CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC 
TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG 
AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG 
GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC 
GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT 
CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC 
CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC 
ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC 
TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC 
ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA 
ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC 
CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG 
TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG 
GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC 
AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG 
CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA 
GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT 
TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG 
ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC 
AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC 
ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC 
CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG 
GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC 
ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA 
GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT 
CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG 
GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA 
TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC 
AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC 
TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG 
AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC 
GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT 
GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG 
ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC 
AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC 
AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT 
TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG 
GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG 
GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA 
GATTTGCCCA GCTCCCCCAG GTACTTAAGA ATCCGACAGC CAAATCTGGA AAGCATCAAT 
TTGGAGTGGG ATCACCCAGA ACATCCCAAT GGAGTCCTCA CGGGATACAA CCTTAGATAT 
CAAGCCTTTA ACGGATCCAA AACGGGCAGA ACCCTGGTAG AGAACTTCTC TCCCAACCAG 
ACAAGGTTCA CCGTGCAGAG GACAGACCCC ATCTCGCGCT ATCGATTCTT CCTGCGTGCT 
CGGACACAGG TGGGAGATGG AGAAGTCATA GTGGAAGAGT CACCAGCGCT CCTGAATGAA 
GCCACGCCAA CCCCAGCCTC CACCTGGTTG CCTCCACCTA CAACCGAGCT AACTCCAGCA 
GCCACCATTG CCACCACAAC CACCACTGCC ACCCCTACTA CTGAAACCCC TCCTACTGAA 
ATCCCTACTA CTGCCATCCC TACCACCACC ACTACTACAA CCACCACAGC TGCCAGCACC 
GTCGCAAGCA CTACAACAAC TGCAGAGAGG GCTGCGGCAG CCACCACAAA ACAGGAGCTG 
GCTTACACCA AGAACCACGT GGACATCGCG ACCCAGGGCT GGTTCATCGG GCTGATGTGT 
GCCATCGCCC TCCTGGTCCT CATTCTGCTG ATTGTTTGCT TCATTAAGAG GAGCAGAGGA 
GGGAAATACC CAGTGCGTGA CAACAAAGAT GAGCACCTGA ATCCTGAAGA CAAGAACGTA 
GAAGATGGCT CATTCGACTA CAGCGATGAA GACAACAAAC CACTGCCCAA CAGTCAGACC 
TCCCTGGATG GCACGATAAA GCAACAGGAG AGTGACGACA GCTTGGTGGA CTACGGAGAG 
GGTGGGGAAG GGCAGTTCAA CGAGGACGGC TCCTTCATTG GCCAGTACAC AGTGAAAAAG 
GACAAGGAGG AAACGGAAGG CAACGAGAGC TCAGAAGCCA CGTCCCCAGT CAATGCTATC 
TACTCATTAG CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Analysis of non-canonical fibroblast growth factor receptor 1 (FGFR1) interaction reveals regulatory and activating domains of neurofascin.
The Journal of biological chemistry284(42)28533-42(2009 Oct)
Kirschbaum K,Kriebel M,Kranz EU,P?tz O,Volkmer H

A regulated switch of chick neurofascin isoforms modulates ligand recognition and neurite extension.
Molecular and cellular neurosciences31(2)354-65(2006 Feb)
Pruss T,Kranz EU,Niere M,Volkmer H

Organization of the neurofascin gene and analysis of developmentally regulated alternative splicing.
The Journal of biological chemistry272(45)28742-9(1997 Nov)
Hassel B,Rathjen FG,Volkmer H

The L1 family of neural cell adhesion molecules: old proteins performing new tricks.
Neuron17(4)587-93(1996 Oct)
Hortsch M

Structure of the axonal surface recognition molecule neurofascin and its relationship to a neural subgroup of the immunoglobulin superfamily.
The Journal of cell biology118(1)149-61(1992 Jul)
Volkmer H,Hassel B,Wolff JM,Frank R,Rathjen FG

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NFASC cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

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