NES cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	NES
Entrez Gene ID	395890
Full Name	nestin
Synonyms	paranemin
General protein information	Preferred Names nestin Names nestin transitin
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_205033.2	NP_990364.2	nestin

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Different transcription factors regulate nestin gene expression during P19 cell neural differentiation and central nervous system development.
Jin Z, Liu L, Bian W, Chen Y, Xu G, Cheng L, Jing N
The Journal of biological chemistry284(12)8160-73(2009 Mar)

Transitin, a nestin-like intermediate filament protein, mediates cortical localization and the lateral transport of Numb in mitotic avian neuroepithelial cells.
Wakamatsu Y, Nakamura N, Lee JA, Cole GJ, Osumi N
Development (Cambridge, England)134(13)2425-33(2007 Jul)

Transitin, a nestin-related intermediate filament, is expressed by neural progenitors and can be induced in M?ller glia in the chicken retina.
Fischer AJ, Omar G
The Journal of comparative neurology484(1)1-14(2005 Mar)

Molecular characteristics of the novel intermediate filament protein paranemin. Sequence reveals EAP-300 and IFAPa-400 are highly homologous to paranemin.
Hemken PM, Bellin RM, Sernett SW, Becker B, Huiatt TW, Robson RM
The Journal of biological chemistry272(51)32489-99(1997 Dec)

Molecular characterization of EAP-300: a high molecular weight, embryonic polypeptide containing an amino acid repeat comprised of multiple leucine-zipper motifs.
Kelly MM, Phanhthourath C, Brees DK, McCabe CF, Cole GJ
Brain research. Developmental brain research85(1)31-47(1995 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sox2 and SF1 may mediate basal nestin expression in undifferentiated cells, whereas Sox2, Brn1, and Brn2 bind to the enhancer in neural progenitor cells.
Title: Different transcription factors regulate nestin gene expression during P19 cell neural differentiation and central nervous system development.

transitin has important roles in determining the intracellular localization of Numb, which regulates neurogenesis in the developing nervous system of avian embryos
Title: Transitin, a nestin-like intermediate filament protein, mediates cortical localization and the lateral transport of Numb in mitotic avian neuroepithelial cells.

transitin is expressed by neural progenitors in the embryonic and postnatal chicken retina
Title: Transitin, a nestin-related intermediate filament, is expressed by neural progenitors and can be induced in M?ller glia in the chicken retina.

The following NES gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NES cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa28393	NM_205033.2 Latest version!	Gallus gallus nestin (NES), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NES ( NM_205033.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990364.2 Gallus gallus nestin (NES), mRNA.

Price & Availability↑

CloneID	OGa28393
Clone ID Related Accession (Same CDS sequence)	NM_205033.2
Accession Version	NM_205033.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-28
Organism	Gallus gallus(chicken)
Product	nestin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U59287.2. On Jun 29, 2015 this sequence version replaced NM_205033.1. ##Evidence-Data-START## Transcript exon combination :: U59287.2 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAGCA TGGAGGGCTT CGTGGGGGCG AGAGCTCTGG GTGAGGAATC GCTGCAGATG 
TGGGACCTCA ACAAGCGCCT GGAGGCCTAC CTGGCTCGCG TCAAGTTCCT GGAGGAGGAG 
AATGAGGGGC TTCGAGCTGA GATCCAGAGC ACCAAGGAGA ACCCGGCCGG GGACCCACGC 
AGGGCCAGGT ATGAGGAGGA GCTGCGGTCG CTGCGGGATG CGCTGCACCG CGCCTTCACC 
GAGAAGTGTG CGGCCGAGCT GGCCAGGGAC AACCTGTATG AGGAGGTGCA GCATGTGAGG 
AGCAGGTGCC AGAAGGAGCA GGCAGCCCGC GAGGAAGCCA AGAGGCAGCT GTCCTCCAGC 
AAGAAGGAGC TGGAGGAGGA GAGACGGGCG CAGATCTGGC TGAAGGAGAG AGCCGTGCAG 
CTGGAGAAGG AGGTGGAAGC TCTGCTGGAG GTGCACGAGG AGGAGAAAGC AGGGCTGGAC 
CAGGAGCTCG CCAGCTTCTC TCAGAGCCTG GAGGGCTTCC GCTGTGCACC GGTGGCATTC 
CAGCCCGTGG AGGTGGAGGA CTACTCCAAA AGACTGTCAG AGATCTGGAG AGGGGCGGTG 
GAGACCTACA AGGCAGAGGT GTCACAGCTG GAGCGTGCAC TTGGCCAAGC CAAGGAGAAC 
CTCTGGCAGG TGGCAGAGGA CAACCAACAG AGCCAACTGC AGCTGCGGCA CCTGGAGAAG 
GAGCTGGTGG GGCTGAAGGT GCGGAAGGAG ATGCTGGAGG AGAGCCTGGG CCAGCAGTGG 
CAGGAGCAGC ACGGAGAGGC TGAGAAGTTC CAGCTGGCCA TCGAGGCCCT GGAGCAGGAG 
AAGCAGAGCC TGCAGGTGCA GATCGCCCAG GTGCTGGAGG ACAGGCAGCA GCTCATGCAC 
CTCAAGATGT CCCTCAGCCT GGAGGTGGCA ACCTACAGGA CACTGCTGGA AGCAGAGAGC 
ACCCGGCTGC AGATGCCGCC CGGGGAGTTC AAGCTGGCCA ACAGCCTGCG AGATGTCAAG 
CTGGAGGCGA GCAGCAGCAA GCACCGTGCC AGCCTTGCTG CCTTCCCCCG GCCTGAGGGG 
GTGGCACAGC TGTGCAGAAC CCCTGGTGAT GCCCTCAAGG TGCTGACCCC CAAAAGCAAG 
AGCTCCAGTG CACTGGAGTT CCAGAAAATC AGCTCGGTCC TGCAGGCACC GAGGAGCTGG 
GAGCCTGCTG CCCCCAGCCC CACTGTCCCA GTGGTGTCCC CAGAGCCAGG CAGTGGGGGG 
GCAGAGAGCC CAGTCCATGA GTGTGGGGCT GGCAAGGAGT CCCCCATGCT GAGCCCACTG 
AGTCCTGAAC AGCTGGTCAA CCACGCGCTG CAGGATGCTC TCAAGGAGAT GCAAGATGAT 
GCTGAGGCCA AAGAAGTGCC CACACTCAGT GCCACCCAGA GTACCAGGGA TGGGGATCTT 
GAAGCCACCA TGGAGGAGGA GGAGGCTGCA GGAACCCAGG GGGTGGGTGC TGAGGGTGAA 
ACCGTGTCCC CACCTGGGCT GTGTTTCTGC AGCAACGAGC CTACATTGTT AAGTGCCACC 
CAGAGTGATG TAGAGAGCCA GGAGGAGATG TGGGAGGAGG AGAGGAGCAA AGAGGAGATG 
CTGAACCCAC TGAGCTCCAT GGAGAGCCAG GAGCCAGGGG GAGAGCCCTG GGGAGGGGTC 
ACAAGGAGGT CCAGGCTGCA GGTGGGCAAG GAGGACATGG AGGCCACCAG CACAGAGGCA 
CTGCACATCT CGGAGAAAAA GGAGCAGAGG GAAATCTGGA GCCCCTCCAG GGAGGATGAG 
GAATGTGAGT TCCCAGATGA GGAGAGGGAA ATGCAAGAAG AAGGGTCCCT GCAGATGGAA 
ATCGAAGCTG CTTGTGCTGT CCCTGTGGGG AGCCACCCAG TTTTGCCAAC AGGAATCCAC 
TTGCAAGAGG ACTTCCTTGA GAGAGAGCAG GAGTCTGAGC ACCAGGAGAC GTCCCTGGGT 
GAGCTGGGTG CTGCTGCAGG GGAGGAGAGG GAGCAGGAGG TGTGCCAGGA GCTGAAGGCT 
TCTAGCATTG AAGAGGCCAT GCCAGCAGCA GAGGGCTCAT CAGGGTCTGG AGAAGGCACC 
ACAGGAAGGG AGAGCACAGG CAGAGCAAGG GATGATGAGG GAGAAGAGGA AGATAAAGGA 
AGGGAGGCAT TAGGGGAAGA TGACCTCCAG GCAGGGGAGG CTTTGGGAGC CAAAGAGCTC 
GGGAAGGAGA GCATGGGCTT GGAGGAGGCT GAGGGCATGT GGGAGGAATC TGTGGACCTG 
CAGGAAGAAC ATAGAGACCT TCAGGAGGGA CATGGGGACC TGCAGGTGGA GCATGAGGAC 
CTGTGGGAGG AACACGGGCA CATACAGGAG GAGCATGGGG ACACCCAGGA GGAATATGGG 
GACACCCAGG AGGAACATGG GGACCTGCAG GTGGAAGGTG GGGACCTGCA GGAGGAGCAT 
GGGGACACCC AGGAGGAGCA TGGGGACCTG CAGGAGGAGC ATGGGGACAC CCAGGAGGAG 
CATGGGGACC TGCAGGTGGA GCATGAGGAC CTGCAGGTGG AGCATGGGGA CCTGTGGGAG 
GAACACAGGG ATGTACAGGA GGAGCATGGG GACACCCAGG AGGAATATGG GGACACCCAG 
GAGGAACATG GGGACCTGCA GGTGGAAGGT GGGGACCTGC AGGAGGAGCA TGGGGACACC 
CAGGAGGAAC ATGAGGACCT GCAGGAGGAG CATGGGGACA CCCAGGAGGA GCATGGGGAC 
CTGCAGGTAG AACATGGGGA CCTGCAGGAG GAACACAGGG ACCTGCAGGA GGGGCATGGG 
GACCTGAAGG AGGAGCATGG GGACCTGCAG GTAGAACATG AGGACCTGCA GGTGGAGCAT 
GGGGACCTAC AGGAGGAGCA TGGGGACACC CAGGAGGAGC ATGGGGACCT GCAGGAGGAG 
CATGGGGACC TGCAGGTGGA ACATGAGGAC CTGCAGGTGG AACATGGGGA CCTGCAGGTG 
GAGCATGGGG ACCTACAGGA GGAGCATGGG GACACCCAGG AGGAACATAG GGATCTGCAG 
GAGGTACATG GAGACCAGCA GGAGGAACAC AGGGACCTGC AGGAGGGGCA TGGGGACCTG 
CAGGAGGAGC ATGGGGACCT GCAGGTGGAA CATGGGGACC TGCAGGAAGA GTATGGGGAC 
ACCCAGGAGG AACATAGGGA TCTGCAGGAG GTACATGGAG ACCAGCAGGA GGAACATAGG 
AACCTGCAGG AGGGGCATGG GGACTTGCAG GAGGGGCATG GGGACTTGCA GGAGGAACAT 
GGGGACCTGT GGAAGGAACA TGGGGTCTTG AAGGAGGAGC ATGGGGACCT GCAGGAGGGG 
CATGGGGACC TGCAGGAGGA ATCTGGGGAC CCTCAGGAGG AACCTGGGGA ACCTTGGGTG 
CAGCATGGGG AGCAAGGCTC TGCAGGGGAT GGGCTGGAGC AGGACATGGT GCTGCAGCCA 
GGAGAGGGGG CATGGGGCAG GGAAGACAAT GACATCAGTC AAAAGCAGCA AGCACAGGAC 
TGGGAAGGGA CAGCGGAGGA TGAAGAGGAG ACAGGAGTCA ATACCATCAC ATCCCAAGAG 
CCAACCCAGG TGGATGACAA CCCACATGCA GAGGCAGCAG AAAATGAGGA GAGGGATGTC 
ACATCACCAA CAGCAATGGA AGAAACCCAG GAGGGTGAGG ATGAGGGAGA TGCTGGGAGT 
GAGGTGCAGA GCCAGCAGCA GCCACAGGAC ACTCCAGGAC AGGAGGCTGA GCCGGCCCCA 
GGGCAGAAGG AGGTCAGGTA TGGGGACACT GGGGAGGCAC CAGGGGACCC ACAGGAGCCA 
GCTGAGGCAC TGGAGGTGGA GGATGAGGAG CTCAGCTCAG AACCCATTGA GCTGGAGAGA 
GGTAGCCCTG ACACTGCAGT GATGCAGCAG GATCTGGGCA ATGGCGCAGA GAGTGATGAG 
CCCATGGAGG AGGACTTTCA GTCAGAGGAT GCTCAGCTGG AGGAGCCCAA GGCATGCAGG 
ATGGAATTAG AAGACACGCT GCTCAACAGC ACGCCGCTAT GTGCATACAG CGGGGAGATG 
CTGGAGTCTG ATCCAAACCC TCCATCCAGC GGAGGGGATG GGGAAGCAGC ACCTGAAATG 
GCTCAGGAGG AGGAAGGGGA CCTGAGAGGG AGCGATGAGG CAGCGGTTCA TGCAGAGCCA 
GAGAGCTGTG AGGAGCTGAG CCCTGCACCA GAATGCACCG AGGAGGAGGA AGGGTACTTT 
ATAGTTTCTG CTCCCAGCCA AGAGGGGTCC AGCATGGAGG AAGCTGAGAA CTCAGAGGAA 
TTTGAAGAAA TTAAAGTTGA AGCAGCAGAA GACAGAAAGG ATGAACTAAC AGCGCCTGGA 
GTAGCATCTT TGGTGCCAGA GGATGAAGGG CACTCGGAGC CATTTGTGGG GGAAGCTGAA 
GATGTGAAAA TGCCCCTTGG GGAATTTGAG ATGCCAAAGG AGGAGGATGA GGAGGATGCA 
GGGGGTTTTG CTGCTGAGCT GGAGGAAGGA CTGACTGTGC CCGTGGCTGA GGGGCTGTCT 
GAGGGGCACA CTGATAAGAC CACCCTGGGT GATGAGGGGC TGGGGGAGGA GGACGTGCAG 
GATGGTGACA ACCCCCCGGC CACTGAGACC TCCGACACTG ATCCCTCCAA CACTGACCCA 
CCTCCAGGCA CCATGCTGGA GCATGGAGCT GGCATGGAGG CGGCTGAATA TCTCCCCGAT 
GTGCCCACGC AACTGCCAGT GGACATCATG AAGGACTCGG ATATTCTGGA GATAGTAGAG 
CAAGCCCTGG AGTTCAATCA GGAGCTGGTA CTGGGAGCCA AGCTGGCCAA GGATGGGCAG 
GAGGAGGATG GTGACGCACA GCCCCCACAA GAGGAGGAAG GGGGGTCCTC ACCCACCTCG 
TCCTGCGATG AGCAGCCAAC GGTGCAGGAG GCGGTGGCAG AGCCAGAGCG CACCAAGAAC 
GGGGAGCAGA ATGGGCTGCA CCGGCAGGCC AGCCTGGAGG ACCTGGCTGA GTTCACCGAG 
GAGGGGCTGA ATGGCATCAC CCACCCTGGG GAAGCCCCTG CTGCCCACAC CCTGCCCCTG 
CCCAGCAAGC ACAGTGGGGC TGAGCCTGTC CCTGAGCTGT CTCCGCTGCA GACCACGTCC 
TGCGCCCGGA GCAGGAGCCC TGGTCCTCTG GGGAGGAGTG ATGCGGTGGG ACCCCACAGC 
CCCGCGACAA TCGGACGCCT CCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990364.2
CDS	25..5271
Translation	MLSMEGFVGARALGEESLQMWDLNKRLEAYLARVKFLEEENEGLRAEIQSTKENPAGDPRRARYEEELRSLRDALHRAFTEKCAAELARDNLYEEVQHVRSRCQKEQAAREEAKRQLSSSKKELEEERRAQIWLKERAVQLEKEVEALLEVHEEEKAGLDQELASFSQSLEGFRCAPVAFQPVEVEDYSKRLSEIWRGAVETYKAEVSQLERALGQAKENLWQVAEDNQQSQLQLRHLEKELVGLKVRKEMLEESLGQQWQEQHGEAEKFQLAIEALEQEKQSLQVQIAQVLEDRQQLMHLKMSLSLEVATYRTLLEAESTRLQMPPGEFKLANSLRDVKLEASSSKHRASLAAFPRPEGVAQLCRTPGDALKVLTPKSKSSSALEFQKISSVLQAPRSWEPAAPSPTVPVVSPEPGSGGAESPVHECGAGKESPMLSPLSPEQLVNHALQDALKEMQDDAEAKEVPTLSATQSTRDGDLEATMEEEEAAGTQGVGAEGETVSPPGLCFCSNEPTLLSATQSDVESQEEMWEEERSKEEMLNPLSSMESQEPGGEPWGGVTRRSRLQVGKEDMEATSTEALHISEKKEQREIWSPSREDEECEFPDEEREMQEEGSLQMEIEAACAVPVGSHPVLPTGIHLQEDFLEREQESEHQETSLGELGAAAGEEREQEVCQELKASSIEEAMPAAEGSSGSGEGTTGRESTGRARDDEGEEEDKGREALGEDDLQAGEALGAKELGKESMGLEEAEGMWEESVDLQEEHRDLQEGHGDLQVEHEDLWEEHGHIQEEHGDTQEEYGDTQEEHGDLQVEGGDLQEEHGDTQEEHGDLQEEHGDTQEEHGDLQVEHEDLQVEHGDLWEEHRDVQEEHGDTQEEYGDTQEEHGDLQVEGGDLQEEHGDTQEEHEDLQEEHGDTQEEHGDLQVEHGDLQEEHRDLQEGHGDLKEEHGDLQVEHEDLQVEHGDLQEEHGDTQEEHGDLQEEHGDLQVEHEDLQVEHGDLQVEHGDLQEEHGDTQEEHRDLQEVHGDQQEEHRDLQEGHGDLQEEHGDLQVEHGDLQEEYGDTQEEHRDLQEVHGDQQEEHRNLQEGHGDLQEGHGDLQEEHGDLWKEHGVLKEEHGDLQEGHGDLQEESGDPQEEPGEPWVQHGEQGSAGDGLEQDMVLQPGEGAWGREDNDISQKQQAQDWEGTAEDEEETGVNTITSQEPTQVDDNPHAEAAENEERDVTSPTAMEETQEGEDEGDAGSEVQSQQQPQDTPGQEAEPAPGQKEVRYGDTGEAPGDPQEPAEALEVEDEELSSEPIELERGSPDTAVMQQDLGNGAESDEPMEEDFQSEDAQLEEPKACRMELEDTLLNSTPLCAYSGEMLESDPNPPSSGGDGEAAPEMAQEEEGDLRGSDEAAVHAEPESCEELSPAPECTEEEEGYFIVSAPSQEGSSMEEAENSEEFEEIKVEAAEDRKDELTAPGVASLVPEDEGHSEPFVGEAEDVKMPLGEFEMPKEEDEEDAGGFAAELEEGLTVPVAEGLSEGHTDKTTLGDEGLGEEDVQDGDNPPATETSDTDPSNTDPPPGTMLEHGAGMEAAEYLPDVPTQLPVDIMKDSDILEIVEQALEFNQELVLGAKLAKDGQEEDGDAQPPQEEEGGSSPTSSCDEQPTVQEAVAEPERTKNGEQNGLHRQASLEDLAEFTEEGLNGITHPGEAPAAHTLPLPSKHSGAEPVPELSPLQTTSCARSRSPGPLGRSDAVGPHSPATIGRLP

Target ORF information:

RefSeq Version	NM_205033.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nestin (NES), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205033.2

ORF Insert Sequence:

ATGCTGAGCA TGGAGGGCTT CGTGGGGGCG AGAGCTCTGG GTGAGGAATC GCTGCAGATG 
TGGGACCTCA ACAAGCGCCT GGAGGCCTAC CTGGCTCGCG TCAAGTTCCT GGAGGAGGAG 
AATGAGGGGC TTCGAGCTGA GATCCAGAGC ACCAAGGAGA ACCCGGCCGG GGACCCACGC 
AGGGCCAGGT ATGAGGAGGA GCTGCGGTCG CTGCGGGATG CGCTGCACCG CGCCTTCACC 
GAGAAGTGTG CGGCCGAGCT GGCCAGGGAC AACCTGTATG AGGAGGTGCA GCATGTGAGG 
AGCAGGTGCC AGAAGGAGCA GGCAGCCCGC GAGGAAGCCA AGAGGCAGCT GTCCTCCAGC 
AAGAAGGAGC TGGAGGAGGA GAGACGGGCG CAGATCTGGC TGAAGGAGAG AGCCGTGCAG 
CTGGAGAAGG AGGTGGAAGC TCTGCTGGAG GTGCACGAGG AGGAGAAAGC AGGGCTGGAC 
CAGGAGCTCG CCAGCTTCTC TCAGAGCCTG GAGGGCTTCC GCTGTGCACC GGTGGCATTC 
CAGCCCGTGG AGGTGGAGGA CTACTCCAAA AGACTGTCAG AGATCTGGAG AGGGGCGGTG 
GAGACCTACA AGGCAGAGGT GTCACAGCTG GAGCGTGCAC TTGGCCAAGC CAAGGAGAAC 
CTCTGGCAGG TGGCAGAGGA CAACCAACAG AGCCAACTGC AGCTGCGGCA CCTGGAGAAG 
GAGCTGGTGG GGCTGAAGGT GCGGAAGGAG ATGCTGGAGG AGAGCCTGGG CCAGCAGTGG 
CAGGAGCAGC ACGGAGAGGC TGAGAAGTTC CAGCTGGCCA TCGAGGCCCT GGAGCAGGAG 
AAGCAGAGCC TGCAGGTGCA GATCGCCCAG GTGCTGGAGG ACAGGCAGCA GCTCATGCAC 
CTCAAGATGT CCCTCAGCCT GGAGGTGGCA ACCTACAGGA CACTGCTGGA AGCAGAGAGC 
ACCCGGCTGC AGATGCCGCC CGGGGAGTTC AAGCTGGCCA ACAGCCTGCG AGATGTCAAG 
CTGGAGGCGA GCAGCAGCAA GCACCGTGCC AGCCTTGCTG CCTTCCCCCG GCCTGAGGGG 
GTGGCACAGC TGTGCAGAAC CCCTGGTGAT GCCCTCAAGG TGCTGACCCC CAAAAGCAAG 
AGCTCCAGTG CACTGGAGTT CCAGAAAATC AGCTCGGTCC TGCAGGCACC GAGGAGCTGG 
GAGCCTGCTG CCCCCAGCCC CACTGTCCCA GTGGTGTCCC CAGAGCCAGG CAGTGGGGGG 
GCAGAGAGCC CAGTCCATGA GTGTGGGGCT GGCAAGGAGT CCCCCATGCT GAGCCCACTG 
AGTCCTGAAC AGCTGGTCAA CCACGCGCTG CAGGATGCTC TCAAGGAGAT GCAAGATGAT 
GCTGAGGCCA AAGAAGTGCC CACACTCAGT GCCACCCAGA GTACCAGGGA TGGGGATCTT 
GAAGCCACCA TGGAGGAGGA GGAGGCTGCA GGAACCCAGG GGGTGGGTGC TGAGGGTGAA 
ACCGTGTCCC CACCTGGGCT GTGTTTCTGC AGCAACGAGC CTACATTGTT AAGTGCCACC 
CAGAGTGATG TAGAGAGCCA GGAGGAGATG TGGGAGGAGG AGAGGAGCAA AGAGGAGATG 
CTGAACCCAC TGAGCTCCAT GGAGAGCCAG GAGCCAGGGG GAGAGCCCTG GGGAGGGGTC 
ACAAGGAGGT CCAGGCTGCA GGTGGGCAAG GAGGACATGG AGGCCACCAG CACAGAGGCA 
CTGCACATCT CGGAGAAAAA GGAGCAGAGG GAAATCTGGA GCCCCTCCAG GGAGGATGAG 
GAATGTGAGT TCCCAGATGA GGAGAGGGAA ATGCAAGAAG AAGGGTCCCT GCAGATGGAA 
ATCGAAGCTG CTTGTGCTGT CCCTGTGGGG AGCCACCCAG TTTTGCCAAC AGGAATCCAC 
TTGCAAGAGG ACTTCCTTGA GAGAGAGCAG GAGTCTGAGC ACCAGGAGAC GTCCCTGGGT 
GAGCTGGGTG CTGCTGCAGG GGAGGAGAGG GAGCAGGAGG TGTGCCAGGA GCTGAAGGCT 
TCTAGCATTG AAGAGGCCAT GCCAGCAGCA GAGGGCTCAT CAGGGTCTGG AGAAGGCACC 
ACAGGAAGGG AGAGCACAGG CAGAGCAAGG GATGATGAGG GAGAAGAGGA AGATAAAGGA 
AGGGAGGCAT TAGGGGAAGA TGACCTCCAG GCAGGGGAGG CTTTGGGAGC CAAAGAGCTC 
GGGAAGGAGA GCATGGGCTT GGAGGAGGCT GAGGGCATGT GGGAGGAATC TGTGGACCTG 
CAGGAAGAAC ATAGAGACCT TCAGGAGGGA CATGGGGACC TGCAGGTGGA GCATGAGGAC 
CTGTGGGAGG AACACGGGCA CATACAGGAG GAGCATGGGG ACACCCAGGA GGAATATGGG 
GACACCCAGG AGGAACATGG GGACCTGCAG GTGGAAGGTG GGGACCTGCA GGAGGAGCAT 
GGGGACACCC AGGAGGAGCA TGGGGACCTG CAGGAGGAGC ATGGGGACAC CCAGGAGGAG 
CATGGGGACC TGCAGGTGGA GCATGAGGAC CTGCAGGTGG AGCATGGGGA CCTGTGGGAG 
GAACACAGGG ATGTACAGGA GGAGCATGGG GACACCCAGG AGGAATATGG GGACACCCAG 
GAGGAACATG GGGACCTGCA GGTGGAAGGT GGGGACCTGC AGGAGGAGCA TGGGGACACC 
CAGGAGGAAC ATGAGGACCT GCAGGAGGAG CATGGGGACA CCCAGGAGGA GCATGGGGAC 
CTGCAGGTAG AACATGGGGA CCTGCAGGAG GAACACAGGG ACCTGCAGGA GGGGCATGGG 
GACCTGAAGG AGGAGCATGG GGACCTGCAG GTAGAACATG AGGACCTGCA GGTGGAGCAT 
GGGGACCTAC AGGAGGAGCA TGGGGACACC CAGGAGGAGC ATGGGGACCT GCAGGAGGAG 
CATGGGGACC TGCAGGTGGA ACATGAGGAC CTGCAGGTGG AACATGGGGA CCTGCAGGTG 
GAGCATGGGG ACCTACAGGA GGAGCATGGG GACACCCAGG AGGAACATAG GGATCTGCAG 
GAGGTACATG GAGACCAGCA GGAGGAACAC AGGGACCTGC AGGAGGGGCA TGGGGACCTG 
CAGGAGGAGC ATGGGGACCT GCAGGTGGAA CATGGGGACC TGCAGGAAGA GTATGGGGAC 
ACCCAGGAGG AACATAGGGA TCTGCAGGAG GTACATGGAG ACCAGCAGGA GGAACATAGG 
AACCTGCAGG AGGGGCATGG GGACTTGCAG GAGGGGCATG GGGACTTGCA GGAGGAACAT 
GGGGACCTGT GGAAGGAACA TGGGGTCTTG AAGGAGGAGC ATGGGGACCT GCAGGAGGGG 
CATGGGGACC TGCAGGAGGA ATCTGGGGAC CCTCAGGAGG AACCTGGGGA ACCTTGGGTG 
CAGCATGGGG AGCAAGGCTC TGCAGGGGAT GGGCTGGAGC AGGACATGGT GCTGCAGCCA 
GGAGAGGGGG CATGGGGCAG GGAAGACAAT GACATCAGTC AAAAGCAGCA AGCACAGGAC 
TGGGAAGGGA CAGCGGAGGA TGAAGAGGAG ACAGGAGTCA ATACCATCAC ATCCCAAGAG 
CCAACCCAGG TGGATGACAA CCCACATGCA GAGGCAGCAG AAAATGAGGA GAGGGATGTC 
ACATCACCAA CAGCAATGGA AGAAACCCAG GAGGGTGAGG ATGAGGGAGA TGCTGGGAGT 
GAGGTGCAGA GCCAGCAGCA GCCACAGGAC ACTCCAGGAC AGGAGGCTGA GCCGGCCCCA 
GGGCAGAAGG AGGTCAGGTA TGGGGACACT GGGGAGGCAC CAGGGGACCC ACAGGAGCCA 
GCTGAGGCAC TGGAGGTGGA GGATGAGGAG CTCAGCTCAG AACCCATTGA GCTGGAGAGA 
GGTAGCCCTG ACACTGCAGT GATGCAGCAG GATCTGGGCA ATGGCGCAGA GAGTGATGAG 
CCCATGGAGG AGGACTTTCA GTCAGAGGAT GCTCAGCTGG AGGAGCCCAA GGCATGCAGG 
ATGGAATTAG AAGACACGCT GCTCAACAGC ACGCCGCTAT GTGCATACAG CGGGGAGATG 
CTGGAGTCTG ATCCAAACCC TCCATCCAGC GGAGGGGATG GGGAAGCAGC ACCTGAAATG 
GCTCAGGAGG AGGAAGGGGA CCTGAGAGGG AGCGATGAGG CAGCGGTTCA TGCAGAGCCA 
GAGAGCTGTG AGGAGCTGAG CCCTGCACCA GAATGCACCG AGGAGGAGGA AGGGTACTTT 
ATAGTTTCTG CTCCCAGCCA AGAGGGGTCC AGCATGGAGG AAGCTGAGAA CTCAGAGGAA 
TTTGAAGAAA TTAAAGTTGA AGCAGCAGAA GACAGAAAGG ATGAACTAAC AGCGCCTGGA 
GTAGCATCTT TGGTGCCAGA GGATGAAGGG CACTCGGAGC CATTTGTGGG GGAAGCTGAA 
GATGTGAAAA TGCCCCTTGG GGAATTTGAG ATGCCAAAGG AGGAGGATGA GGAGGATGCA 
GGGGGTTTTG CTGCTGAGCT GGAGGAAGGA CTGACTGTGC CCGTGGCTGA GGGGCTGTCT 
GAGGGGCACA CTGATAAGAC CACCCTGGGT GATGAGGGGC TGGGGGAGGA GGACGTGCAG 
GATGGTGACA ACCCCCCGGC CACTGAGACC TCCGACACTG ATCCCTCCAA CACTGACCCA 
CCTCCAGGCA CCATGCTGGA GCATGGAGCT GGCATGGAGG CGGCTGAATA TCTCCCCGAT 
GTGCCCACGC AACTGCCAGT GGACATCATG AAGGACTCGG ATATTCTGGA GATAGTAGAG 
CAAGCCCTGG AGTTCAATCA GGAGCTGGTA CTGGGAGCCA AGCTGGCCAA GGATGGGCAG 
GAGGAGGATG GTGACGCACA GCCCCCACAA GAGGAGGAAG GGGGGTCCTC ACCCACCTCG 
TCCTGCGATG AGCAGCCAAC GGTGCAGGAG GCGGTGGCAG AGCCAGAGCG CACCAAGAAC 
GGGGAGCAGA ATGGGCTGCA CCGGCAGGCC AGCCTGGAGG ACCTGGCTGA GTTCACCGAG 
GAGGGGCTGA ATGGCATCAC CCACCCTGGG GAAGCCCCTG CTGCCCACAC CCTGCCCCTG 
CCCAGCAAGC ACAGTGGGGC TGAGCCTGTC CCTGAGCTGT CTCCGCTGCA GACCACGTCC 
TGCGCCCGGA GCAGGAGCCC TGGTCCTCTG GGGAGGAGTG ATGCGGTGGG ACCCCACAGC 
CCCGCGACAA TCGGACGCCT CCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Different transcription factors regulate nestin gene expression during P19 cell neural differentiation and central nervous system development.
The Journal of biological chemistry284(12)8160-73(2009 Mar)
Jin Z,Liu L,Bian W,Chen Y,Xu G,Cheng L,Jing N

Transitin, a nestin-like intermediate filament protein, mediates cortical localization and the lateral transport of Numb in mitotic avian neuroepithelial cells.
Development (Cambridge, England)134(13)2425-33(2007 Jul)
Wakamatsu Y,Nakamura N,Lee JA,Cole GJ,Osumi N

Transitin, a nestin-related intermediate filament, is expressed by neural progenitors and can be induced in M?ller glia in the chicken retina.
The Journal of comparative neurology484(1)1-14(2005 Mar)
Fischer AJ,Omar G

Molecular characteristics of the novel intermediate filament protein paranemin. Sequence reveals EAP-300 and IFAPa-400 are highly homologous to paranemin.
The Journal of biological chemistry272(51)32489-99(1997 Dec)
Hemken PM,Bellin RM,Sernett SW,Becker B,Huiatt TW,Robson RM

Molecular characterization of EAP-300: a high molecular weight, embryonic polypeptide containing an amino acid repeat comprised of multiple leucine-zipper motifs.
Brain research. Developmental brain research85(1)31-47(1995 Mar)
Kelly MM,Phanhthourath C,Brees DK,McCabe CF,Cole GJ

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NES cDNA ORF clone, Gallus gallus(chicken)

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