NPHP4 gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_006069607.1	XP_006069669.1	nephrocystin-4 isoform X6
XM_025285820.1	XP_025141605.1	nephrocystin-4 isoform X5
XM_006069603.1	XP_006069665.1	nephrocystin-4 isoform X2
XM_006069604.1	XP_006069666.1	nephrocystin-4 isoform X3
XM_025285815.1	XP_025141600.1	nephrocystin-4 isoform X1
XM_025285818.1	XP_025141603.1	nephrocystin-4 isoform X3
XM_006069602.2	XP_006069664.2	nephrocystin-4 isoform X6
XM_025285822.1	XP_025141607.1	nephrocystin-4 isoform X8
XM_025285821.1	XP_025141606.1	nephrocystin-4 isoform X7
XM_006069605.1	XP_006069667.1	nephrocystin-4 isoform X4
XM_006069606.1	XP_006069668.1	nephrocystin-4 isoform X5
XM_025285817.1	XP_025141602.1	nephrocystin-4 isoform X2
XM_006069603.2	XP_006069665.2	nephrocystin-4 isoform X6
XM_006069602.1	XP_006069664.1	nephrocystin-4 isoform X1
XM_025285819.1	XP_025141604.1	nephrocystin-4 isoform X4
XM_006069604.2	XP_006069666.2	nephrocystin-4 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu24353	XM_006069602.1 Latest version!	Bubalus bubalis nephronophthisis 4 (NPHP4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu24353	XM_006069604.1 Latest version!	Bubalus bubalis nephronophthisis 4 (NPHP4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu24353	XM_006069603.1 Latest version!	Bubalus bubalis nephronophthisis 4 (NPHP4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu24354	XM_006069605.1 Latest version!	Bubalus bubalis nephronophthisis 4 (NPHP4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu24355	XM_006069606.1 Latest version!	Bubalus bubalis nephronophthisis 4 (NPHP4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu24356	XM_006069607.1 Latest version!	Bubalus bubalis nephronophthisis 4 (NPHP4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu66236	XM_025285818.1 Latest version!	Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu66237	XM_025285819.1 Latest version!	Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu66238	XM_025285820.1 Latest version!	Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu66239	XM_006069604.2 Latest version!	Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu66239	XM_006069603.2 Latest version!	Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu66239	XM_006069602.2 Latest version!	Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu66240	XM_025285821.1 Latest version!	Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu66241	XM_025285822.1 Latest version!	Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu66234	XM_025285815.1 Latest version!	Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu66235	XM_025285817.1 Latest version!	Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

NPHP4 ( XM_006069603.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006069665.1 Bubalus bubalis nephronophthisis 4 (NPHP4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu24353
Clone ID Related Accession (Same CDS sequence)	XM_006069603.1 , XM_006069604.1 , XM_006069602.1
Accession Version	XM_006069603.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	nephrocystin-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785355.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCAGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACAGCG TTTCAGTGTG TCCTGAAGTG GCTCGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATTGGAGG TGGACTGCCA CCTGCGTGTG 
TCCTTCTTCG ATGTCACCTA TCGGCACTTC TTTGGGAGGA CGTGGAAGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGG 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCACGGG 
AGCCCGAGAG CCCTCCTCCA CCCACTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT TCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACCTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGTCTC 
CTTCCAGCTC ACGGAGAGTC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAACTTCTGG ATCTTGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC TCTGGATGTC 
GGCGCCTTGG AGATCCTGGA GAGGCGCCTG TGCGTGGGCG TGCACAATGG TTTGGGGTTT 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCCTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCATC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCGG ACGGCAATGC AGCTTCACTC 
ACCTCCTTGT CCAGCCTGGC ATGCATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
TTGGAAGCCA GCTCCGGACA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATGA GCTCTGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGACACA 
CCCACAAGGA ATGCAAGCGG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGCG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTCCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCGCT TTCCCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCACGC 
CGCCGCTCGG CCAAACCCTC ATCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC GCCTGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTATC ACGGGCGTCC 
CTGGCGCTGC TGCAGTCTGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GCTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACAC CACGACTGCG GCTCGTGGAG CTGGCCGATG CTGGGAAAAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT TGTAGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATTGACG TCTGGGATGG GGACTCCCTG 
CTTCTCGTCG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCAGCC 
GTGCAGGTCT CGCACGAGCT GGAGGTTGTG GCGACTGAAT ATGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACAGTGGTG 
AAGGGCCAGC TGCACTTGAC ATTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTCGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCCCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACCCCCAAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAGT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAGTGTGGAT CGGAGCCGGG CCGCCGCGGG 
CTGGGCGTGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC GAAGGCGGAG AGCATCGCCA GTGCGCTGAG CATGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCACTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACACGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTA CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAAATCAGAG AAGGACACGG ATGCTGGATC ACTCTATAAG 
TCCAGCATGA TGCCCACCAA ACATGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTAGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAGGCCATCC GCCTGCCGCC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCACGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAAT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCGGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC CGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
CGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTCG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCACGGGGAG CGGGAGGGGC GCCAACAAGC GCATCACCTA CACCAACCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGCGAG ACCTATACCA TCGGCCTGCG GTTTGCGTCC 
CGTCAGAGAG CTGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006069665.1
CDS	138..4433
Translation	MHDWHRIFDQNQLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLLEVDCHLRVSFFDVTYRHFFGRTWKSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLDLVISDCFREAGSPLDVGALEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVHHPAFAIVFQLEYVFNSPSGADGNAASLTSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASMSSEEVKQVESGTVQFQYSLSPEEHPDTPTRNASGPKAERRSFRKPPASPSSPPASPSQGLTAPQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLRLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLVGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTPPRPGARGPQGISQEGDAVRRRKLERMRAVRLRECGSEPGRRGLGVLAQQSLHAQHARDLQVIAAYRERTKAESIASALSMAITTQHTIHATLGTAEFFEFALRNPHNAQHTVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLYLRPRETAHIPFKFQTFSVGPWASAQASAELKSEKDTDAGSLYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQNVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPRRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPTGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFASRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_006069603.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis nephronophthisis 4 (NPHP4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006069603.1

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCAGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACAGCG TTTCAGTGTG TCCTGAAGTG GCTCGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATTGGAGG TGGACTGCCA CCTGCGTGTG 
TCCTTCTTCG ATGTCACCTA TCGGCACTTC TTTGGGAGGA CGTGGAAGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGG 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCACGGG 
AGCCCGAGAG CCCTCCTCCA CCCACTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT TCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACCTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGTCTC 
CTTCCAGCTC ACGGAGAGTC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAACTTCTGG ATCTTGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC TCTGGATGTC 
GGCGCCTTGG AGATCCTGGA GAGGCGCCTG TGCGTGGGCG TGCACAATGG TTTGGGGTTT 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCCTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCATC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCGG ACGGCAATGC AGCTTCACTC 
ACCTCCTTGT CCAGCCTGGC ATGCATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
TTGGAAGCCA GCTCCGGACA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATGA GCTCTGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGACACA 
CCCACAAGGA ATGCAAGCGG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGCG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTCCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCGCT TTCCCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCACGC 
CGCCGCTCGG CCAAACCCTC ATCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC GCCTGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTATC ACGGGCGTCC 
CTGGCGCTGC TGCAGTCTGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GCTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACAC CACGACTGCG GCTCGTGGAG CTGGCCGATG CTGGGAAAAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT TGTAGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATTGACG TCTGGGATGG GGACTCCCTG 
CTTCTCGTCG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCAGCC 
GTGCAGGTCT CGCACGAGCT GGAGGTTGTG GCGACTGAAT ATGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACAGTGGTG 
AAGGGCCAGC TGCACTTGAC ATTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTCGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCCCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACCCCCAAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAGT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAGTGTGGAT CGGAGCCGGG CCGCCGCGGG 
CTGGGCGTGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC GAAGGCGGAG AGCATCGCCA GTGCGCTGAG CATGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCACTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACACGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTA CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAAATCAGAG AAGGACACGG ATGCTGGATC ACTCTATAAG 
TCCAGCATGA TGCCCACCAA ACATGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTAGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAGGCCATCC GCCTGCCGCC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCACGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAAT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCGGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC CGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
CGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTCG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCACGGGGAG CGGGAGGGGC GCCAACAAGC GCATCACCTA CACCAACCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGCGAG ACCTATACCA TCGGCCTGCG GTTTGCGTCC 
CGTCAGAGAG CTGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_006069604.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006069666.1 Bubalus bubalis nephronophthisis 4 (NPHP4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu24353
Clone ID Related Accession (Same CDS sequence)	XM_006069603.1 , XM_006069604.1 , XM_006069602.1
Accession Version	XM_006069604.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	nephrocystin-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785355.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCAGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACAGCG TTTCAGTGTG TCCTGAAGTG GCTCGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATTGGAGG TGGACTGCCA CCTGCGTGTG 
TCCTTCTTCG ATGTCACCTA TCGGCACTTC TTTGGGAGGA CGTGGAAGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGG 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCACGGG 
AGCCCGAGAG CCCTCCTCCA CCCACTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT TCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACCTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGTCTC 
CTTCCAGCTC ACGGAGAGTC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAACTTCTGG ATCTTGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC TCTGGATGTC 
GGCGCCTTGG AGATCCTGGA GAGGCGCCTG TGCGTGGGCG TGCACAATGG TTTGGGGTTT 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCCTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCATC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCGG ACGGCAATGC AGCTTCACTC 
ACCTCCTTGT CCAGCCTGGC ATGCATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
TTGGAAGCCA GCTCCGGACA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATGA GCTCTGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGACACA 
CCCACAAGGA ATGCAAGCGG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGCG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTCCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCGCT TTCCCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCACGC 
CGCCGCTCGG CCAAACCCTC ATCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC GCCTGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTATC ACGGGCGTCC 
CTGGCGCTGC TGCAGTCTGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GCTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACAC CACGACTGCG GCTCGTGGAG CTGGCCGATG CTGGGAAAAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT TGTAGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATTGACG TCTGGGATGG GGACTCCCTG 
CTTCTCGTCG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCAGCC 
GTGCAGGTCT CGCACGAGCT GGAGGTTGTG GCGACTGAAT ATGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACAGTGGTG 
AAGGGCCAGC TGCACTTGAC ATTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTCGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCCCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACCCCCAAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAGT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAGTGTGGAT CGGAGCCGGG CCGCCGCGGG 
CTGGGCGTGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC GAAGGCGGAG AGCATCGCCA GTGCGCTGAG CATGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCACTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACACGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTA CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAAATCAGAG AAGGACACGG ATGCTGGATC ACTCTATAAG 
TCCAGCATGA TGCCCACCAA ACATGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTAGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAGGCCATCC GCCTGCCGCC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCACGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAAT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCGGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC CGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
CGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTCG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCACGGGGAG CGGGAGGGGC GCCAACAAGC GCATCACCTA CACCAACCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGCGAG ACCTATACCA TCGGCCTGCG GTTTGCGTCC 
CGTCAGAGAG CTGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006069666.1
CDS	578..4873
Translation	MHDWHRIFDQNQLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLLEVDCHLRVSFFDVTYRHFFGRTWKSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLDLVISDCFREAGSPLDVGALEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVHHPAFAIVFQLEYVFNSPSGADGNAASLTSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASMSSEEVKQVESGTVQFQYSLSPEEHPDTPTRNASGPKAERRSFRKPPASPSSPPASPSQGLTAPQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLRLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLVGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTPPRPGARGPQGISQEGDAVRRRKLERMRAVRLRECGSEPGRRGLGVLAQQSLHAQHARDLQVIAAYRERTKAESIASALSMAITTQHTIHATLGTAEFFEFALRNPHNAQHTVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLYLRPRETAHIPFKFQTFSVGPWASAQASAELKSEKDTDAGSLYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQNVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPRRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPTGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFASRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_006069604.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis nephronophthisis 4 (NPHP4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006069604.1

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCAGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACAGCG TTTCAGTGTG TCCTGAAGTG GCTCGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATTGGAGG TGGACTGCCA CCTGCGTGTG 
TCCTTCTTCG ATGTCACCTA TCGGCACTTC TTTGGGAGGA CGTGGAAGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGG 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCACGGG 
AGCCCGAGAG CCCTCCTCCA CCCACTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT TCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACCTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGTCTC 
CTTCCAGCTC ACGGAGAGTC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAACTTCTGG ATCTTGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC TCTGGATGTC 
GGCGCCTTGG AGATCCTGGA GAGGCGCCTG TGCGTGGGCG TGCACAATGG TTTGGGGTTT 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCCTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCATC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCGG ACGGCAATGC AGCTTCACTC 
ACCTCCTTGT CCAGCCTGGC ATGCATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
TTGGAAGCCA GCTCCGGACA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATGA GCTCTGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGACACA 
CCCACAAGGA ATGCAAGCGG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGCG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTCCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCGCT TTCCCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCACGC 
CGCCGCTCGG CCAAACCCTC ATCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC GCCTGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTATC ACGGGCGTCC 
CTGGCGCTGC TGCAGTCTGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GCTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACAC CACGACTGCG GCTCGTGGAG CTGGCCGATG CTGGGAAAAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT TGTAGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATTGACG TCTGGGATGG GGACTCCCTG 
CTTCTCGTCG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCAGCC 
GTGCAGGTCT CGCACGAGCT GGAGGTTGTG GCGACTGAAT ATGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACAGTGGTG 
AAGGGCCAGC TGCACTTGAC ATTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTCGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCCCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACCCCCAAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAGT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAGTGTGGAT CGGAGCCGGG CCGCCGCGGG 
CTGGGCGTGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC GAAGGCGGAG AGCATCGCCA GTGCGCTGAG CATGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCACTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACACGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTA CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAAATCAGAG AAGGACACGG ATGCTGGATC ACTCTATAAG 
TCCAGCATGA TGCCCACCAA ACATGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTAGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAGGCCATCC GCCTGCCGCC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCACGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAAT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCGGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC CGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
CGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTCG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCACGGGGAG CGGGAGGGGC GCCAACAAGC GCATCACCTA CACCAACCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGCGAG ACCTATACCA TCGGCCTGCG GTTTGCGTCC 
CGTCAGAGAG CTGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_006069602.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006069664.1 Bubalus bubalis nephronophthisis 4 (NPHP4), transcript variant X1, mRNA.

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CloneID	OBu24353
Clone ID Related Accession (Same CDS sequence)	XM_006069603.1 , XM_006069604.1 , XM_006069602.1
Accession Version	XM_006069602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	nephrocystin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785355.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCAGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACAGCG TTTCAGTGTG TCCTGAAGTG GCTCGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATTGGAGG TGGACTGCCA CCTGCGTGTG 
TCCTTCTTCG ATGTCACCTA TCGGCACTTC TTTGGGAGGA CGTGGAAGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGG 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCACGGG 
AGCCCGAGAG CCCTCCTCCA CCCACTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT TCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACCTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGTCTC 
CTTCCAGCTC ACGGAGAGTC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAACTTCTGG ATCTTGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC TCTGGATGTC 
GGCGCCTTGG AGATCCTGGA GAGGCGCCTG TGCGTGGGCG TGCACAATGG TTTGGGGTTT 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCCTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCATC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCGG ACGGCAATGC AGCTTCACTC 
ACCTCCTTGT CCAGCCTGGC ATGCATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
TTGGAAGCCA GCTCCGGACA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATGA GCTCTGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGACACA 
CCCACAAGGA ATGCAAGCGG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGCG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTCCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCGCT TTCCCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCACGC 
CGCCGCTCGG CCAAACCCTC ATCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC GCCTGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTATC ACGGGCGTCC 
CTGGCGCTGC TGCAGTCTGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GCTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACAC CACGACTGCG GCTCGTGGAG CTGGCCGATG CTGGGAAAAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT TGTAGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATTGACG TCTGGGATGG GGACTCCCTG 
CTTCTCGTCG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCAGCC 
GTGCAGGTCT CGCACGAGCT GGAGGTTGTG GCGACTGAAT ATGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACAGTGGTG 
AAGGGCCAGC TGCACTTGAC ATTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTCGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCCCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACCCCCAAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAGT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAGTGTGGAT CGGAGCCGGG CCGCCGCGGG 
CTGGGCGTGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC GAAGGCGGAG AGCATCGCCA GTGCGCTGAG CATGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCACTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACACGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTA CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAAATCAGAG AAGGACACGG ATGCTGGATC ACTCTATAAG 
TCCAGCATGA TGCCCACCAA ACATGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTAGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAGGCCATCC GCCTGCCGCC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCACGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAAT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCGGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC CGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
CGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTCG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCACGGGGAG CGGGAGGGGC GCCAACAAGC GCATCACCTA CACCAACCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGCGAG ACCTATACCA TCGGCCTGCG GTTTGCGTCC 
CGTCAGAGAG CTGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006069664.1
CDS	292..4587
Translation	MHDWHRIFDQNQLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLLEVDCHLRVSFFDVTYRHFFGRTWKSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLDLVISDCFREAGSPLDVGALEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVHHPAFAIVFQLEYVFNSPSGADGNAASLTSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASMSSEEVKQVESGTVQFQYSLSPEEHPDTPTRNASGPKAERRSFRKPPASPSSPPASPSQGLTAPQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLRLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLVGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTPPRPGARGPQGISQEGDAVRRRKLERMRAVRLRECGSEPGRRGLGVLAQQSLHAQHARDLQVIAAYRERTKAESIASALSMAITTQHTIHATLGTAEFFEFALRNPHNAQHTVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLYLRPRETAHIPFKFQTFSVGPWASAQASAELKSEKDTDAGSLYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQNVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPRRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPTGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFASRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_006069602.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis nephronophthisis 4 (NPHP4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006069602.1

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCAGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACAGCG TTTCAGTGTG TCCTGAAGTG GCTCGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATTGGAGG TGGACTGCCA CCTGCGTGTG 
TCCTTCTTCG ATGTCACCTA TCGGCACTTC TTTGGGAGGA CGTGGAAGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGG 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCACGGG 
AGCCCGAGAG CCCTCCTCCA CCCACTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT TCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACCTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGTCTC 
CTTCCAGCTC ACGGAGAGTC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAACTTCTGG ATCTTGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC TCTGGATGTC 
GGCGCCTTGG AGATCCTGGA GAGGCGCCTG TGCGTGGGCG TGCACAATGG TTTGGGGTTT 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCCTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCATC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCGG ACGGCAATGC AGCTTCACTC 
ACCTCCTTGT CCAGCCTGGC ATGCATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
TTGGAAGCCA GCTCCGGACA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATGA GCTCTGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGACACA 
CCCACAAGGA ATGCAAGCGG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGCG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTCCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCGCT TTCCCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCACGC 
CGCCGCTCGG CCAAACCCTC ATCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC GCCTGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTATC ACGGGCGTCC 
CTGGCGCTGC TGCAGTCTGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GCTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACAC CACGACTGCG GCTCGTGGAG CTGGCCGATG CTGGGAAAAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT TGTAGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATTGACG TCTGGGATGG GGACTCCCTG 
CTTCTCGTCG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCAGCC 
GTGCAGGTCT CGCACGAGCT GGAGGTTGTG GCGACTGAAT ATGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACAGTGGTG 
AAGGGCCAGC TGCACTTGAC ATTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTCGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCCCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACCCCCAAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAGT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAGTGTGGAT CGGAGCCGGG CCGCCGCGGG 
CTGGGCGTGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC GAAGGCGGAG AGCATCGCCA GTGCGCTGAG CATGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCACTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACACGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTA CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAAATCAGAG AAGGACACGG ATGCTGGATC ACTCTATAAG 
TCCAGCATGA TGCCCACCAA ACATGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTAGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAGGCCATCC GCCTGCCGCC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCACGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAAT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCGGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC CGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
CGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTCG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCACGGGGAG CGGGAGGGGC GCCAACAAGC GCATCACCTA CACCAACCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGCGAG ACCTATACCA TCGGCCTGCG GTTTGCGTCC 
CGTCAGAGAG CTGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_006069605.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006069667.1 Bubalus bubalis nephronophthisis 4 (NPHP4), transcript variant X4, mRNA.

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CloneID	OBu24354
Clone ID Related Accession (Same CDS sequence)	XM_006069605.1
Accession Version	XM_006069605.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	nephrocystin-4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785355.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCAGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACAGCG TTTCAGTGTG TCCTGAAGTG GCTCGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATTGGAGG TGGACTGCCA CCTGCGTGTG 
TCCTTCTTCG ATGTCACCTA TCGGCACTTC TTTGGGAGGA CGTGGAAGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGG 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCACGGG 
AGCCCGAGAG CCCTCCTCCA CCCACTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT TCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACCTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGTCTC 
CTTCCAGCTC ACGGAGAGTC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAACTTCTGG ATCTTGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC TCTGGATGTC 
GGCGCCTTGG AGATCCTGGA GAGGCGCCTG TGCGTGGGCG TGCACAATGG TTTGGGGTTT 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCCTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCATC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCGG ACGGCAATGC AGCTTCACTC 
ACCTCCTTGT CCAGCCTGGC ATGCATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
TTGGAAGCCA GCTCCGGACA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATGA GCTCTGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGACACA 
CCCACAAGGA ATGCAAGCGG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGCG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTCCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCGCT TTCCCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCACGC 
CGCCGCTCGG CCAAACCCTC ATCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC GCCTGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTATC ACGGGCGTCC 
CTGGCGCTGC TGCAGTCTGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GCTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACAC CACGACTGCG GCTCGTGGAG CTGGCCGATG CTGGGAAAAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT TGTAGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATTGACG TCTGGGATGG GGACTCCCTG 
CTTCTCGTCG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCAGCC 
GTGCAGGTCT CGCACGAGCT GGAGGTTGTG GCGACTGAAT ATGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACAGTGGTG 
AAGGGCCAGC TGCACTTGAC ATTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTCGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT TGAAAAATGT GGTCCAAGCC 
CAGAAGCTGG CTGATGTGGA CAGCGAACTG GCCGCCCTGC TCTTCACACC CCCAAGACCA 
GGGGCCCGGG GGCCCCAGGG CATCAGTCAA GAGGGGGATG CCGTCCGCAG GCGCAAGCTG 
GAGCGCATGA GGGCCGTGCG GCTGCGGGAG TGTGGATCGG AGCCGGGCCG CCGCGGGCTG 
GGCGTGCTGG CCCAGCAGAG CCTCCATGCC CAGCACGCCC GAGACCTGCA GGTCATTGCC 
GCCTACCGTG AGCGCACGAA GGCGGAGAGC ATCGCCAGTG CGCTGAGCAT GGCCATCACC 
ACGCAGCACA CCATCCACGC CACCCTAGGC ACTGCCGAGT TCTTCGAGTT CGCCCTGAGG 
AACCCCCACA ACGCGCAGCA CACGGTGACC ATCGAGATAG ACAACCCCGA GCTCAGTCTC 
ATCCTGGATG GTCAGGAGTG GCGGTACTTC AAGGACATGG CTGACTTGCA CACACCCCTG 
GAGGAGGACA TGTTCCACCT GCGGGGCAGC CTGGCCCCCC AACTCTACCT GCGACCTCGG 
GAGACTGCCC ACATCCCCTT CAAGTTCCAG ACTTTCTCTG TGGGCCCCTG GGCCTCGGCA 
CAGGCCTCTG CTGAGCTGAA ATCAGAGAAG GACACGGATG CTGGATCACT CTATAAGTCC 
AGCATGATGC CCACCAAACA TGCCAAGGTC CTGTTCCGCG CGGGCAGCGG CAAGCCCCTG 
GCCGTGCTCT GCCTGACGGT GGAGCTGCAG CCTCACGTGG TAGACCAGGT CTTCCGCTTC 
CACCACCCGG AGCTCACCTT CCTGAAGAAG GCCATCCGCC TGCCGCCCTG GCACACGCTT 
CCAGGTGCCC CTGTAGGGAC TTCCAGTGAG GACCCCCCTT TCCACGTTCG ATGTAGTGAC 
CCGAACATCA TTTGTGAGAC ACAGAATGTG GGCCCCGGGG AGCCCCGTGA CGTGTTCCTG 
AAGGTGGCCA GTGGCCCTAG CCCGGAGGTC AAGGACTTCT TTGTCACCAT CTACGTAGAC 
CGCTGGTTAG CAACACCCGT CCAGACCTGG CAGGTCCACC TGCACTCGCT GCAGCGAGTG 
GACGTCTCCT GTATCACTGG CCAGAGGACC CGCCTGGCTC TCGTCCTCCG GGGGACGCAG 
ACAGTTAGGA GAGTGCAGGC TTTCAGCTCA CACCCCCAGG AGCTGACGAC GGACCCCAGA 
GGTGTGTTCG TGCTGCCTCC TCACGGGGTG CAGGACTTGC ATGTGAGCGT CAGGCCCCGC 
AGGCCGGGCA GCCGCTTCGC GCACCTCCAC CTGGTCGACG TGGACGCCCA CCAGCTGGTG 
GCCTCGTGGC TCCTGTGTCT CTCCTGCCGG CCGCCTCTCA TCTCCAAGGC CTTCGAGATA 
AAGCTGCCCA CGGGGAGCGG GAGGGGCGCC AACAAGCGCA TCACCTACAC CAACCCCTAC 
CCCTCGCGGA GGGCCTACCG CCTGCACAGC GACCACCCCC ACCTGCTGCA GTTCAAGGAG 
GACGCCTTCC AGGTCGGGGG AGGCGAGACC TATACCATCG GCCTGCGGTT TGCGTCCCGT 
CAGAGAGCTG GGGAGGAAGA GATCCTGATT TACATCAACG ACCACGAAGA CAAAAACGAG 
GAGACCTTTT GCGTGAAGGT CATCTACCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006069667.1
CDS	292..4584
Translation	MHDWHRIFDQNQLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLLEVDCHLRVSFFDVTYRHFFGRTWKSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLDLVISDCFREAGSPLDVGALEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVHHPAFAIVFQLEYVFNSPSGADGNAASLTSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASMSSEEVKQVESGTVQFQYSLSPEEHPDTPTRNASGPKAERRSFRKPPASPSSPPASPSQGLTAPQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLRLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLVGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRLKNVVQAQKLADVDSELAALLFTPPRPGARGPQGISQEGDAVRRRKLERMRAVRLRECGSEPGRRGLGVLAQQSLHAQHARDLQVIAAYRERTKAESIASALSMAITTQHTIHATLGTAEFFEFALRNPHNAQHTVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLYLRPRETAHIPFKFQTFSVGPWASAQASAELKSEKDTDAGSLYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQNVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPRRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPTGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFASRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_006069605.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis nephronophthisis 4 (NPHP4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006069605.1

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCAGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACAGCG TTTCAGTGTG TCCTGAAGTG GCTCGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATTGGAGG TGGACTGCCA CCTGCGTGTG 
TCCTTCTTCG ATGTCACCTA TCGGCACTTC TTTGGGAGGA CGTGGAAGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGG 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCACGGG 
AGCCCGAGAG CCCTCCTCCA CCCACTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT TCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACCTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGTCTC 
CTTCCAGCTC ACGGAGAGTC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAACTTCTGG ATCTTGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC TCTGGATGTC 
GGCGCCTTGG AGATCCTGGA GAGGCGCCTG TGCGTGGGCG TGCACAATGG TTTGGGGTTT 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCCTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCATC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCGG ACGGCAATGC AGCTTCACTC 
ACCTCCTTGT CCAGCCTGGC ATGCATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
TTGGAAGCCA GCTCCGGACA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATGA GCTCTGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGACACA 
CCCACAAGGA ATGCAAGCGG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGCG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTCCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCGCT TTCCCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCACGC 
CGCCGCTCGG CCAAACCCTC ATCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC GCCTGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTATC ACGGGCGTCC 
CTGGCGCTGC TGCAGTCTGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GCTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACAC CACGACTGCG GCTCGTGGAG CTGGCCGATG CTGGGAAAAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT TGTAGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATTGACG TCTGGGATGG GGACTCCCTG 
CTTCTCGTCG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCAGCC 
GTGCAGGTCT CGCACGAGCT GGAGGTTGTG GCGACTGAAT ATGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACAGTGGTG 
AAGGGCCAGC TGCACTTGAC ATTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTCGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT TGAAAAATGT GGTCCAAGCC 
CAGAAGCTGG CTGATGTGGA CAGCGAACTG GCCGCCCTGC TCTTCACACC CCCAAGACCA 
GGGGCCCGGG GGCCCCAGGG CATCAGTCAA GAGGGGGATG CCGTCCGCAG GCGCAAGCTG 
GAGCGCATGA GGGCCGTGCG GCTGCGGGAG TGTGGATCGG AGCCGGGCCG CCGCGGGCTG 
GGCGTGCTGG CCCAGCAGAG CCTCCATGCC CAGCACGCCC GAGACCTGCA GGTCATTGCC 
GCCTACCGTG AGCGCACGAA GGCGGAGAGC ATCGCCAGTG CGCTGAGCAT GGCCATCACC 
ACGCAGCACA CCATCCACGC CACCCTAGGC ACTGCCGAGT TCTTCGAGTT CGCCCTGAGG 
AACCCCCACA ACGCGCAGCA CACGGTGACC ATCGAGATAG ACAACCCCGA GCTCAGTCTC 
ATCCTGGATG GTCAGGAGTG GCGGTACTTC AAGGACATGG CTGACTTGCA CACACCCCTG 
GAGGAGGACA TGTTCCACCT GCGGGGCAGC CTGGCCCCCC AACTCTACCT GCGACCTCGG 
GAGACTGCCC ACATCCCCTT CAAGTTCCAG ACTTTCTCTG TGGGCCCCTG GGCCTCGGCA 
CAGGCCTCTG CTGAGCTGAA ATCAGAGAAG GACACGGATG CTGGATCACT CTATAAGTCC 
AGCATGATGC CCACCAAACA TGCCAAGGTC CTGTTCCGCG CGGGCAGCGG CAAGCCCCTG 
GCCGTGCTCT GCCTGACGGT GGAGCTGCAG CCTCACGTGG TAGACCAGGT CTTCCGCTTC 
CACCACCCGG AGCTCACCTT CCTGAAGAAG GCCATCCGCC TGCCGCCCTG GCACACGCTT 
CCAGGTGCCC CTGTAGGGAC TTCCAGTGAG GACCCCCCTT TCCACGTTCG ATGTAGTGAC 
CCGAACATCA TTTGTGAGAC ACAGAATGTG GGCCCCGGGG AGCCCCGTGA CGTGTTCCTG 
AAGGTGGCCA GTGGCCCTAG CCCGGAGGTC AAGGACTTCT TTGTCACCAT CTACGTAGAC 
CGCTGGTTAG CAACACCCGT CCAGACCTGG CAGGTCCACC TGCACTCGCT GCAGCGAGTG 
GACGTCTCCT GTATCACTGG CCAGAGGACC CGCCTGGCTC TCGTCCTCCG GGGGACGCAG 
ACAGTTAGGA GAGTGCAGGC TTTCAGCTCA CACCCCCAGG AGCTGACGAC GGACCCCAGA 
GGTGTGTTCG TGCTGCCTCC TCACGGGGTG CAGGACTTGC ATGTGAGCGT CAGGCCCCGC 
AGGCCGGGCA GCCGCTTCGC GCACCTCCAC CTGGTCGACG TGGACGCCCA CCAGCTGGTG 
GCCTCGTGGC TCCTGTGTCT CTCCTGCCGG CCGCCTCTCA TCTCCAAGGC CTTCGAGATA 
AAGCTGCCCA CGGGGAGCGG GAGGGGCGCC AACAAGCGCA TCACCTACAC CAACCCCTAC 
CCCTCGCGGA GGGCCTACCG CCTGCACAGC GACCACCCCC ACCTGCTGCA GTTCAAGGAG 
GACGCCTTCC AGGTCGGGGG AGGCGAGACC TATACCATCG GCCTGCGGTT TGCGTCCCGT 
CAGAGAGCTG GGGAGGAAGA GATCCTGATT TACATCAACG ACCACGAAGA CAAAAACGAG 
GAGACCTTTT GCGTGAAGGT CATCTACCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_006069606.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006069668.1 Bubalus bubalis nephronophthisis 4 (NPHP4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu24355
Clone ID Related Accession (Same CDS sequence)	XM_006069606.1
Accession Version	XM_006069606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	nephrocystin-4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785355.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCAGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACAGCG TTTCAGTGTG TCCTGAAGTG GCTCGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATTGGAGG TGGACTGCCA CCTGCGTGTG 
TCCTTCTTCG ATGTCACCTA TCGGCACTTC TTTGGGAGGA CGTGGAAGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGG 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCACGGG 
AGCCCGAGAG CCCTCCTCCA CCCACTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT TCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACCTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGTCTC 
CTTCCAGCTC ACGGAGAGTC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAACTTCTGG ATCTTGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC TCTGGATGTC 
GGCGCCTTGG AGATCCTGGA GAGGCGCCTG TGCGTGGGCG TGCACAATGG TTTGGGGTTT 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCCTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCATC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCGG ACGGCAATGC AGCTTCACTC 
ACCTCCTTGT CCAGCCTGGC ATGCATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
TTGGAAGCCA GCTCCGGACA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATGA GCTCTGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGACACA 
CCCACAAGGA ATGCAAGCGG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGCG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTCCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCGCT TTCCCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCACGC 
CGCCGCTCGG CCAAACCCTC ATCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGAGTTC 
CCCTTGGAGG GCAGGATCTC TCACCTGGAG GCTGACCTGA GCCAGACAGC CTTAGCCCTG 
GGAACGTCCG CTGCTGACCA CTTGCAGGAA CTGCCTTTCA CGCCTGTGCA CGCCCCGATC 
ATTGCAGGAA CCCAGACCAG GAGCTCTGGA GGGAAGCTAT CACGGGCGTC CCTGGCGCTG 
CTGCAGTCTG CTGGCTTTCC CGAGATTCTG GACGCCAACA AGCAACCAGC TGAGGCTGTG 
AGTCCTACAG ACCCCGTGAA GTTCAGCCCT CAGAAGGAAG AATCAGACTG TCTACAAGGC 
AATGAGATAG TGCTGCAATT TCTTGCCTTT AGCAGGGTGC CCCAGGATGA GCAGGCAGCG 
CCATGGCCAC AGACTGTGTA CTTCACCTTC CAATTCTACC GCTTCCCACC CGAGACCACA 
CCACGACTGC GGCTCGTGGA GCTGGCCGAT GCTGGGAAAA CCAGCTCTGC CTCCCTGTCG 
CACATCCTCG CCCAGATCCA GAAGGATGGC TCCTTTGATG CGGGGTCTCC GGGCTTTCAG 
CGGAAGTACC TTGTAGACCC CGGGTTCCTG AGACCCGGGG AGCAGCGCTG GTTCGCCCGC 
TACCTGGCCA TGCAGACCCT GCAGATTGAC GTCTGGGATG GGGACTCCCT GCTTCTCGTC 
GGATCTGCTG CCGTTCAGCT GAAGCACCTT CTCCGCCAGG GGCGGCCAGC CGTGCAGGTC 
TCGCACGAGC TGGAGGTTGT GGCGACTGAA TATGAGCAGG ACGCCATGGT GGTGAGTGGA 
GACATGACCA AGTTTGGCTG CGTCAGACCC ATCGACGTCC ACACAGTGGT GAAGGGCCAG 
CTGCACTTGA CATTGGCCAA CGTGGGTCAC TTGTGTGAAC AGAGAGTAAG GAGTCCCAGT 
TCCCTGCCAC CTTCCAGGTC GAGGGTCATC TCAAATGATG GAGTCGGCAA CTTCATGGGA 
GGCAGCCTCC TGACACCCGG GGGCCCCAGA TTGAAAAATG TGGTCCAAGC CCAGAAGCTG 
GCTGATGTGG ACAGCGAACT GGCCGCCCTG CTCTTCACAC CCCCAAGACC AGGGGCCCGG 
GGGCCCCAGG GCATCAGTCA AGAGGGGGAT GCCGTCCGCA GGCGCAAGCT GGAGCGCATG 
AGGGCCGTGC GGCTGCGGGA GTGTGGATCG GAGCCGGGCC GCCGCGGGCT GGGCGTGCTG 
GCCCAGCAGA GCCTCCATGC CCAGCACGCC CGAGACCTGC AGGTCATTGC CGCCTACCGT 
GAGCGCACGA AGGCGGAGAG CATCGCCAGT GCGCTGAGCA TGGCCATCAC CACGCAGCAC 
ACCATCCACG CCACCCTAGG CACTGCCGAG TTCTTCGAGT TCGCCCTGAG GAACCCCCAC 
AACGCGCAGC ACACGGTGAC CATCGAGATA GACAACCCCG AGCTCAGTCT CATCCTGGAT 
GGTCAGGAGT GGCGGTACTT CAAGGACATG GCTGACTTGC ACACACCCCT GGAGGAGGAC 
ATGTTCCACC TGCGGGGCAG CCTGGCCCCC CAACTCTACC TGCGACCTCG GGAGACTGCC 
CACATCCCCT TCAAGTTCCA GACTTTCTCT GTGGGCCCCT GGGCCTCGGC ACAGGCCTCT 
GCTGAGCTGA AATCAGAGAA GGACACGGAT GCTGGATCAC TCTATAAGTC CAGCATGATG 
CCCACCAAAC ATGCCAAGGT CCTGTTCCGC GCGGGCAGCG GCAAGCCCCT GGCCGTGCTC 
TGCCTGACGG TGGAGCTGCA GCCTCACGTG GTAGACCAGG TCTTCCGCTT CCACCACCCG 
GAGCTCACCT TCCTGAAGAA GGCCATCCGC CTGCCGCCCT GGCACACGCT TCCAGGTGCC 
CCTGTAGGGA CTTCCAGTGA GGACCCCCCT TTCCACGTTC GATGTAGTGA CCCGAACATC 
ATTTGTGAGA CACAGAATGT GGGCCCCGGG GAGCCCCGTG ACGTGTTCCT GAAGGTGGCC 
AGTGGCCCTA GCCCGGAGGT CAAGGACTTC TTTGTCACCA TCTACGTAGA CCGCTGGTTA 
GCAACACCCG TCCAGACCTG GCAGGTCCAC CTGCACTCGC TGCAGCGAGT GGACGTCTCC 
TGTATCACTG GCCAGAGGAC CCGCCTGGCT CTCGTCCTCC GGGGGACGCA GACAGTTAGG 
AGAGTGCAGG CTTTCAGCTC ACACCCCCAG GAGCTGACGA CGGACCCCAG AGGTGTGTTC 
GTGCTGCCTC CTCACGGGGT GCAGGACTTG CATGTGAGCG TCAGGCCCCG CAGGCCGGGC 
AGCCGCTTCG CGCACCTCCA CCTGGTCGAC GTGGACGCCC ACCAGCTGGT GGCCTCGTGG 
CTCCTGTGTC TCTCCTGCCG GCCGCCTCTC ATCTCCAAGG CCTTCGAGAT AAAGCTGCCC 
ACGGGGAGCG GGAGGGGCGC CAACAAGCGC ATCACCTACA CCAACCCCTA CCCCTCGCGG 
AGGGCCTACC GCCTGCACAG CGACCACCCC CACCTGCTGC AGTTCAAGGA GGACGCCTTC 
CAGGTCGGGG GAGGCGAGAC CTATACCATC GGCCTGCGGT TTGCGTCCCG TCAGAGAGCT 
GGGGAGGAAG AGATCCTGAT TTACATCAAC GACCACGAAG ACAAAAACGA GGAGACCTTT 
TGCGTGAAGG TCATCTACCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006069668.1
CDS	293..4576
Translation	MHDWHRIFDQNQLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLLEVDCHLRVSFFDVTYRHFFGRTWKSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLDLVISDCFREAGSPLDVGALEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVHHPAFAIVFQLEYVFNSPSGADGNAASLTSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASMSSEEVKQVESGTVQFQYSLSPEEHPDTPTRNASGPKAERRSFRKPPASPSSPPASPSQGLTAPQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLRLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLVGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRLKNVVQAQKLADVDSELAALLFTPPRPGARGPQGISQEGDAVRRRKLERMRAVRLRECGSEPGRRGLGVLAQQSLHAQHARDLQVIAAYRERTKAESIASALSMAITTQHTIHATLGTAEFFEFALRNPHNAQHTVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLYLRPRETAHIPFKFQTFSVGPWASAQASAELKSEKDTDAGSLYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQNVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPRRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPTGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFASRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_006069606.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis nephronophthisis 4 (NPHP4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006069606.1

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCAGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACAGCG TTTCAGTGTG TCCTGAAGTG GCTCGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATTGGAGG TGGACTGCCA CCTGCGTGTG 
TCCTTCTTCG ATGTCACCTA TCGGCACTTC TTTGGGAGGA CGTGGAAGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGG 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCACGGG 
AGCCCGAGAG CCCTCCTCCA CCCACTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT TCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACCTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGTCTC 
CTTCCAGCTC ACGGAGAGTC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAACTTCTGG ATCTTGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC TCTGGATGTC 
GGCGCCTTGG AGATCCTGGA GAGGCGCCTG TGCGTGGGCG TGCACAATGG TTTGGGGTTT 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCCTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCATC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCGG ACGGCAATGC AGCTTCACTC 
ACCTCCTTGT CCAGCCTGGC ATGCATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
TTGGAAGCCA GCTCCGGACA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATGA GCTCTGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGACACA 
CCCACAAGGA ATGCAAGCGG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGCG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTCCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCGCT TTCCCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCACGC 
CGCCGCTCGG CCAAACCCTC ATCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGAGTTC 
CCCTTGGAGG GCAGGATCTC TCACCTGGAG GCTGACCTGA GCCAGACAGC CTTAGCCCTG 
GGAACGTCCG CTGCTGACCA CTTGCAGGAA CTGCCTTTCA CGCCTGTGCA CGCCCCGATC 
ATTGCAGGAA CCCAGACCAG GAGCTCTGGA GGGAAGCTAT CACGGGCGTC CCTGGCGCTG 
CTGCAGTCTG CTGGCTTTCC CGAGATTCTG GACGCCAACA AGCAACCAGC TGAGGCTGTG 
AGTCCTACAG ACCCCGTGAA GTTCAGCCCT CAGAAGGAAG AATCAGACTG TCTACAAGGC 
AATGAGATAG TGCTGCAATT TCTTGCCTTT AGCAGGGTGC CCCAGGATGA GCAGGCAGCG 
CCATGGCCAC AGACTGTGTA CTTCACCTTC CAATTCTACC GCTTCCCACC CGAGACCACA 
CCACGACTGC GGCTCGTGGA GCTGGCCGAT GCTGGGAAAA CCAGCTCTGC CTCCCTGTCG 
CACATCCTCG CCCAGATCCA GAAGGATGGC TCCTTTGATG CGGGGTCTCC GGGCTTTCAG 
CGGAAGTACC TTGTAGACCC CGGGTTCCTG AGACCCGGGG AGCAGCGCTG GTTCGCCCGC 
TACCTGGCCA TGCAGACCCT GCAGATTGAC GTCTGGGATG GGGACTCCCT GCTTCTCGTC 
GGATCTGCTG CCGTTCAGCT GAAGCACCTT CTCCGCCAGG GGCGGCCAGC CGTGCAGGTC 
TCGCACGAGC TGGAGGTTGT GGCGACTGAA TATGAGCAGG ACGCCATGGT GGTGAGTGGA 
GACATGACCA AGTTTGGCTG CGTCAGACCC ATCGACGTCC ACACAGTGGT GAAGGGCCAG 
CTGCACTTGA CATTGGCCAA CGTGGGTCAC TTGTGTGAAC AGAGAGTAAG GAGTCCCAGT 
TCCCTGCCAC CTTCCAGGTC GAGGGTCATC TCAAATGATG GAGTCGGCAA CTTCATGGGA 
GGCAGCCTCC TGACACCCGG GGGCCCCAGA TTGAAAAATG TGGTCCAAGC CCAGAAGCTG 
GCTGATGTGG ACAGCGAACT GGCCGCCCTG CTCTTCACAC CCCCAAGACC AGGGGCCCGG 
GGGCCCCAGG GCATCAGTCA AGAGGGGGAT GCCGTCCGCA GGCGCAAGCT GGAGCGCATG 
AGGGCCGTGC GGCTGCGGGA GTGTGGATCG GAGCCGGGCC GCCGCGGGCT GGGCGTGCTG 
GCCCAGCAGA GCCTCCATGC CCAGCACGCC CGAGACCTGC AGGTCATTGC CGCCTACCGT 
GAGCGCACGA AGGCGGAGAG CATCGCCAGT GCGCTGAGCA TGGCCATCAC CACGCAGCAC 
ACCATCCACG CCACCCTAGG CACTGCCGAG TTCTTCGAGT TCGCCCTGAG GAACCCCCAC 
AACGCGCAGC ACACGGTGAC CATCGAGATA GACAACCCCG AGCTCAGTCT CATCCTGGAT 
GGTCAGGAGT GGCGGTACTT CAAGGACATG GCTGACTTGC ACACACCCCT GGAGGAGGAC 
ATGTTCCACC TGCGGGGCAG CCTGGCCCCC CAACTCTACC TGCGACCTCG GGAGACTGCC 
CACATCCCCT TCAAGTTCCA GACTTTCTCT GTGGGCCCCT GGGCCTCGGC ACAGGCCTCT 
GCTGAGCTGA AATCAGAGAA GGACACGGAT GCTGGATCAC TCTATAAGTC CAGCATGATG 
CCCACCAAAC ATGCCAAGGT CCTGTTCCGC GCGGGCAGCG GCAAGCCCCT GGCCGTGCTC 
TGCCTGACGG TGGAGCTGCA GCCTCACGTG GTAGACCAGG TCTTCCGCTT CCACCACCCG 
GAGCTCACCT TCCTGAAGAA GGCCATCCGC CTGCCGCCCT GGCACACGCT TCCAGGTGCC 
CCTGTAGGGA CTTCCAGTGA GGACCCCCCT TTCCACGTTC GATGTAGTGA CCCGAACATC 
ATTTGTGAGA CACAGAATGT GGGCCCCGGG GAGCCCCGTG ACGTGTTCCT GAAGGTGGCC 
AGTGGCCCTA GCCCGGAGGT CAAGGACTTC TTTGTCACCA TCTACGTAGA CCGCTGGTTA 
GCAACACCCG TCCAGACCTG GCAGGTCCAC CTGCACTCGC TGCAGCGAGT GGACGTCTCC 
TGTATCACTG GCCAGAGGAC CCGCCTGGCT CTCGTCCTCC GGGGGACGCA GACAGTTAGG 
AGAGTGCAGG CTTTCAGCTC ACACCCCCAG GAGCTGACGA CGGACCCCAG AGGTGTGTTC 
GTGCTGCCTC CTCACGGGGT GCAGGACTTG CATGTGAGCG TCAGGCCCCG CAGGCCGGGC 
AGCCGCTTCG CGCACCTCCA CCTGGTCGAC GTGGACGCCC ACCAGCTGGT GGCCTCGTGG 
CTCCTGTGTC TCTCCTGCCG GCCGCCTCTC ATCTCCAAGG CCTTCGAGAT AAAGCTGCCC 
ACGGGGAGCG GGAGGGGCGC CAACAAGCGC ATCACCTACA CCAACCCCTA CCCCTCGCGG 
AGGGCCTACC GCCTGCACAG CGACCACCCC CACCTGCTGC AGTTCAAGGA GGACGCCTTC 
CAGGTCGGGG GAGGCGAGAC CTATACCATC GGCCTGCGGT TTGCGTCCCG TCAGAGAGCT 
GGGGAGGAAG AGATCCTGAT TTACATCAAC GACCACGAAG ACAAAAACGA GGAGACCTTT 
TGCGTGAAGG TCATCTACCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_006069607.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006069669.1 Bubalus bubalis nephronophthisis 4 (NPHP4), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBu24356
Clone ID Related Accession (Same CDS sequence)	XM_006069607.1
Accession Version	XM_006069607.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	nephrocystin-4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785355.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCAGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACAGCG TTTCAGTGTG TCCTGAAGTG GCTCGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATTGGAGG TGGACTGCCA CCTGCGTGTG 
TCCTTCTTCG ATGTCACCTA TCGGCACTTC TTTGGGAGGA CGTGGAAGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGG 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCACGGG 
AGCCCGAGAG CCCTCCTCCA CCCACTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT TCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACCTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGTCTC 
CTTCCAGCTC ACGGAGAGTC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAACTTCTGG ATCTTGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC TCTGGATGTC 
GGCGCCTTGG AGATCCTGGA GAGGCGCCTG TGCGTGGGCG TGCACAATGG TTTGGGGTTT 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCCTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCATC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCGG ACGGCAATGC AGCTTCACTC 
ACCTCCTTGT CCAGCCTGGC ATGCATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
TTGGAAGCCA GCTCCGGACA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATGA GCTCTGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGACACA 
CCCACAAGGA ATGCAAGCGG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGCG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTCCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCGCT TTCCCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCACGC 
CGCCGCTCGG CCAAACCCTC ATCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC GCCTGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTATC ACGGGCGTCC 
CTGGCGCTGC TGCAGTCTGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GCTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACAC CACGACTGCG GCTCGTGGAG CTGGCCGATG CTGGGAAAAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT TGTAGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATTGACG TCTGGGATGG GGACTCCCTG 
CTTCTCGTCG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCAGCC 
GTGCAGGTCT CGCACGAGCT GGAGGTTGTG GCGACTGAAT ATGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACAGTGGTG 
AAGGGCCAGC TGCACTTGAC ATTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTCGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCCCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACCCCCAAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAGT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAGTGTGGAT CGGAGCCGGG CCGCCGCGGG 
CTGGGCGTGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC GAAGGCGGAG AGCATCGCCA GTGCGCTGAG CATGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCACTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACACGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTA CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAAATCAGAG AAGGACACGG ATGCTGGATC ACTCTATAAG 
TCCAGCATGA TGCCCACCAA ACATGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTAGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAGGCCATCC GCCTGCCGCC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCACGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAAT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCGGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC CGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GGTGGTCACC 
CCTGAAGAGT TGTGCTCCCT GGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006069669.1
CDS	294..4160
Translation	MHDWHRIFDQNQLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLLEVDCHLRVSFFDVTYRHFFGRTWKSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLDLVISDCFREAGSPLDVGALEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVHHPAFAIVFQLEYVFNSPSGADGNAASLTSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASMSSEEVKQVESGTVQFQYSLSPEEHPDTPTRNASGPKAERRSFRKPPASPSSPPASPSQGLTAPQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLRLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLVGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTPPRPGARGPQGISQEGDAVRRRKLERMRAVRLRECGSEPGRRGLGVLAQQSLHAQHARDLQVIAAYRERTKAESIASALSMAITTQHTIHATLGTAEFFEFALRNPHNAQHTVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLYLRPRETAHIPFKFQTFSVGPWASAQASAELKSEKDTDAGSLYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQNVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTVVTPEELCSLG

Target ORF information:

RefSeq Version	XM_006069607.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis nephronophthisis 4 (NPHP4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006069607.1

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCAGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACAGCG TTTCAGTGTG TCCTGAAGTG GCTCGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATTGGAGG TGGACTGCCA CCTGCGTGTG 
TCCTTCTTCG ATGTCACCTA TCGGCACTTC TTTGGGAGGA CGTGGAAGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGG 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCACGGG 
AGCCCGAGAG CCCTCCTCCA CCCACTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT TCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACCTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGTCTC 
CTTCCAGCTC ACGGAGAGTC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAACTTCTGG ATCTTGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC TCTGGATGTC 
GGCGCCTTGG AGATCCTGGA GAGGCGCCTG TGCGTGGGCG TGCACAATGG TTTGGGGTTT 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCCTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCATC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCGG ACGGCAATGC AGCTTCACTC 
ACCTCCTTGT CCAGCCTGGC ATGCATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
TTGGAAGCCA GCTCCGGACA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATGA GCTCTGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGACACA 
CCCACAAGGA ATGCAAGCGG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGCG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTCCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCGCT TTCCCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCACGC 
CGCCGCTCGG CCAAACCCTC ATCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC GCCTGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTATC ACGGGCGTCC 
CTGGCGCTGC TGCAGTCTGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GCTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACAC CACGACTGCG GCTCGTGGAG CTGGCCGATG CTGGGAAAAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT TGTAGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATTGACG TCTGGGATGG GGACTCCCTG 
CTTCTCGTCG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCAGCC 
GTGCAGGTCT CGCACGAGCT GGAGGTTGTG GCGACTGAAT ATGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACAGTGGTG 
AAGGGCCAGC TGCACTTGAC ATTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTCGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCCCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACCCCCAAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAGT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAGTGTGGAT CGGAGCCGGG CCGCCGCGGG 
CTGGGCGTGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC GAAGGCGGAG AGCATCGCCA GTGCGCTGAG CATGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCACTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACACGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTA CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAAATCAGAG AAGGACACGG ATGCTGGATC ACTCTATAAG 
TCCAGCATGA TGCCCACCAA ACATGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTAGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAGGCCATCC GCCTGCCGCC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCACGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAAT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCGGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC CGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GGTGGTCACC 
CCTGAAGAGT TGTGCTCCCT GGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_025285818.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141603.1 Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X3, mRNA.

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CloneID	OBu66236
Clone ID Related Accession (Same CDS sequence)	XM_025285818.1
Accession Version	XM_025285818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	nephrocystin-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGC CGTCGCCGCC AGTTCCAAGC CGGGTCTCCA TCATAGCTCC CTGCACCCTC 
CTCTTCTCTG TGATTTTTAT AGGTCACAAC CACCTCTTTT GTGTGCTAAC TTGTATCCTG 
TGTTTCTGCC TCCCTTCAGA TACATTCCTC GAGGTCAACG TGGTTGTCTG CAGTGTTCTC 
CAGGGCCTGG CACAGGGTAA TCCCAGAGGC CCCAGGGACC TGGGGACTGG CAGAATGCAC 
GACTGGCACA GGATCTTTGA CCAAAACCAG CTGGTCCCTC CGCACCCACA GAGAGCACGC 
CAGCCTCTGA AGGAATCGAC AGCGTTTCAG TGTGTCCTGA AGTGGCTCGA TGGCCCGCTA 
ATTAAGCAGG GGGTGCTGGA GCTGCTATTG GAGGTGGACT GCCACCTGCG CGTGTCCCTC 
TTCGATGTCA CCTATCGGCA CTTCTTTGGG AGGACGTGGA AGAGCTCGCC GAGGCCTGCA 
GAGCCGCTCA CCAGGCAGCC GGCCAGGATC GTCTTCAATG AGCCCTTGTA TTTTCACACG 
TCCTTGAACC ACCCTCATAT TGTGGCCGTG GTGGAGGTGG TCGTTGAAGG CAGGAGACGA 
GACGGGACCC TCCAGACTCT GTCCTGTGGG TTTGGAATTC TTCGGATCTT CAGCCACAAG 
CCGGAATCTC CAACTTCCAC TTCCCAGGAC AAACGGCTGA GACTGTATCA CGGGAGCCCG 
AGAGCCCTCC TCCACCCACT CCTTCAGGAC CCCATAGAAC AGAACAAGCA CATGACCCTC 
ATTGAGAACT GCAGCCTGCA GTACACGCTG AGGCCACACC CGCTTCTGGA GCCCGTGTTC 
CACCTTCTTC CCGAGAACCT GCTGGTGTCC GGTCTGCAGC AGATACCTGG TCTCCTTCCA 
GCTCACGGAG AGTCAGGAGA TGCCCTCCGG AAGCCCCGCC TGCAGAAGCC TCTGACGTGC 
TACCTGGATG ATCTGTTCTT CACGCTGTAT CCCTCGCTGG AGAAGTTCGA GGAGGAACTT 
CTGGATCTTG TGATCAGCGA TTGCTTCCGG GAGGCGGGCA GCCCTCTGGA TGTCGGCGCC 
TTGGAGATCC TGGAGAGGCG CCTGTGCGTG GGCGTGCACA ATGGTTTGGG GTTTGTGCAG 
CGGCCGCAGG TCCTCGTGCT GGTGCCCGAG CTGGAGGTGG CCTTGACACG CTCTGCCAGC 
TTCAGCCGGA AGGTCGGCTC CTCTTCCAAG GCCAGCTCTG GAAACCAGGC GCTGGTCCTG 
AGAAGCCGTC TCCGACTCCC TGAGATGGTC CATCACCCCG CGTTCGCCAT TGTCTTCCAG 
CTGGAGTACG TGTTTAACAG CCCCTCGGGG GCGGACGGCA ATGCAGCTTC ACTCACCTCC 
TTGTCCAGCC TGGCATGCAT GCACATGGTT CGCTGGGCTG TCTGGAACCC CTCGTTGGAA 
GCCAGCTCCG GACAGGTGAT GTTACCTCTC CAGGGTGGCA TCCAGTCCAA CCCTTCGCAC 
TGTCTGGTCT ATAAGGTACC CCCAGCCAGC ATGAGCTCTG AAGAGGTGAA GCAGGTGGAG 
TCAGGGACGG TCCAGTTCCA GTACTCGCTG AGCCCTGAAG AACACCCAGA CACACCCACA 
AGGAATGCAA GCGGCCCCAA GGCAGAGCGG CGGTCTTTTA GGAAGCCACC CGCGTCCCCT 
TCAAGTCCAC CAGCATCGCC GTCCCAGGGG CTCACCGCTC CCCAGGACTC ACCCATGGGA 
CCAGGGCTGT CGCTTTCCCA GCTGGCGGCT TCCCCACCAT CCCCAGCTCC ACGCCGCCGC 
TCGGCCAAAC CCTCATCACA GCCACCTGGG AGCCCTGTGC CCTCCCTGGT CCCAGGGGAG 
TTCCCCTTGG AGGGCAGGAT CTCTCACCTG GAGGCTGACC TGAGCCAGAC AGCCTTAGCC 
CTGGGAACGT CCGCTGCTGA CCACTTGCAG GAACTGCCTT TCACGCCTGT GCACGCCCCG 
ATCATTGCAG GAACCCAGAC CAGGAGCTCT GGAGGGAAGC TATCACGGGC GTCCCTGGCG 
CTGCTGCAGT CTGCTGGCTT TCCCGAGATT CTGGACGCCA ACAAGCAACC AGCTGAGGCT 
GTGAGTCCTA CAGACCCCGT GAAGTTCAGC CCTCAGAAGG AAGAATCAGA CTGTCTACAA 
GGCAATGAGA TAGTGCTGCA ATTTCTTGCC TTTAGCAGGG TGCCCCAGGA TGAGCAGGCA 
GCGCCATGGC CACAGACTGT GTACTTCACC TTCCAATTCT ACCGCTTCCC ACCCGAGACC 
ACACCACGAC TGCGGCTCGT GGAGCTGGCC GATGCTGGGA AAACCAGCTC TGCCTCCCTG 
TCGCACATCC TCGCCCAGAT CCAGAAGGAT GGCTCCTTTG ATGCGGGGTC TCCGGGCTTT 
CAGCGGAAGT ACCTTGTAGA CCCCGGGTTC CTGAGACCCG GGGAGCAGCG CTGGTTCGCC 
CGCTACCTGG CCATGCAGAC CCTGCAGATT GACGTCTGGG ATGGGGACTC CCTGCTTCTC 
GTCGGATCTG CTGCCGTTCA GCTGAAGCAC CTTCTCCGCC AGGGGCGGCC AGCCGTGCAG 
GTCTCGCACG AGCTGGAGGT TGTGGCGACT GAATATGAGC AGGACGCCAT GGTGGTGAGT 
GGAGACATGA CCAAGTTTGG CTGCGTCAGA CCCATCGACG TCCACACAGT GGTGAAGGGC 
CAGCTGCACT TGACATTGGC CAACGTGGCA GTGAAAAATG TGGTCCAAGC CCAGAAGCTG 
GCTGATGTGG ACAGCGAACT GGCCGCCCTG CTCTTCACAC CCCCAAGACC AGGGGCCCGG 
GGGCCCCAGG GCATCAGTCA AGAGGGGGAT GCCGTCCGCA GGCGCAAGCT GGAGCGCATG 
AGGGCCGTGC GGCTGCGGGA GTGTGGATCG GAGCCGGGCC GCCGCGGGCT GGGCGTGCTG 
GCCCAGCAGA GCCTCCATGC CCAGCACGCC CGAGACCTGC AGGTCATTGC CGCCTACCGT 
GAGCGCACGA AGGCGGAGAG CATCGCCAGT GCGCTGAGCA TGGCCATCAC CACGCAGCAC 
ACCATCCACG CCACCCTAGG CACTGCCGAG TTCTTCGAGT TCGCCCTGAG GAACCCCCAC 
AACGCGCAGC ACACGGTGAC CATCGAGATA GACAACCCCG AGCTCAGTCT CATCCTGGAT 
GGTCAGGAGT GGCGGTACTT CAAGGACATG GCTGACTTGC ACACACCCCT GGAGGAGGAC 
ATGTTCCACC TGCGGGGCAG CCTGGCCCCC CAACTCTACC TGCGACCTCG GGAGACTGCC 
CACATCCCCT TCAAGTTCCA GACTTTCTCT GTGGGCCCCT GGGCCTCGGC ACAGGCCTCT 
GCTGAGCTGA AATCAGAGAA GGACACGGAT GCTGGATCAC TCTATAAGTC CAGCATGATG 
CCCACCAAAC ATGCCAAGGT CCTGTTCCGC GCGGGCAGCG GCAAGCCCCT GGCCGTGCTC 
TGCCTGACGG TGGAGCTGCA GCCTCACGTG GTAGACCAGG TCTTCCGCTT CCACCACCCG 
GAGCTCACCT TCCTGAAGAA GGCCATCCGC CTGCCGCCCT GGCACACGCT TCCAGGTGCC 
CCTGTAGGGA CTTCCAGTGA GGACCCCCCT TTCCACGTTC GATGTAGTGA CCCGAACATC 
ATTTGTGAGA CACAGAATGT GGGCCCCGGG GAGCCCCGTG ACGTGTTCCT GAAGGTGGCC 
AGTGGCCCTA GCCCGGAGGT CAAGGACTTC TTTGTCACCA TCTACGTAGA CCGCTGGTTA 
GCAACACCCG TCCAGACCTG GCAGGTCCAC CTGCACTCGC TGCAGCGAGT GGACGTCTCC 
TGTATCACTG GCCAGAGGAC CCGCCTGGCT CTCGTCCTCC GGGGGACGCA GACAGTTAGG 
AGAGTGCAGG CTTTCAGCTC ACACCCCCAG GAGCTGACGA CGGACCCCAG AGGTGTGTTC 
GTGCTGCCTC CTCACGGGGT GCAGGACTTG CATGTGAGCG TCAGGCCCCG CAGGCCGGGC 
AGCCGCTTCG CGCACCTCCA CCTGGTCGAC GTGGACGCCC ACCAGCTGGT GGCCTCGTGG 
CTCCTGTGTC TCTCCTGCCG GCCGCCTCTC ATCTCCAAGG CCTTCGAGAT AAAGCTGCCC 
ACGGGGAGCG GGAGGGGCGC CAACAAGCGC ATCACCTACA CCAACCCCTA CCCCTCGCGG 
AGGGCCTACC GCCTGCACAG CGACCACCCC CACCTGCTGC AGTTCAAGGA GGACGCCTTC 
CAGGTCGGGG GAGGCGAGAC CTATACCATC GGCCTGCGGT TTGCGTCCCG TCAGAGAGCT 
GGGGAGGAAG AGATCCTGAT TTACATCAAC GACCACGAAG ACAAAAACGA GGAGACCTTT 
TGCGTGAAGG TCATCTACCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141603.1
CDS	41..4444
Translation	MIVPSPPVPSRVSIIAPCTLLFSVIFIGHNHLFCVLTCILCFCLPSDTFLEVNVVVCSVLQGLAQGNPRGPRDLGTGRMHDWHRIFDQNQLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLLEVDCHLRVSLFDVTYRHFFGRTWKSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLDLVISDCFREAGSPLDVGALEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVHHPAFAIVFQLEYVFNSPSGADGNAASLTSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASMSSEEVKQVESGTVQFQYSLSPEEHPDTPTRNASGPKAERRSFRKPPASPSSPPASPSQGLTAPQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLRLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLVGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVAVKNVVQAQKLADVDSELAALLFTPPRPGARGPQGISQEGDAVRRRKLERMRAVRLRECGSEPGRRGLGVLAQQSLHAQHARDLQVIAAYRERTKAESIASALSMAITTQHTIHATLGTAEFFEFALRNPHNAQHTVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLYLRPRETAHIPFKFQTFSVGPWASAQASAELKSEKDTDAGSLYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQNVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPRRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPTGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFASRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_025285818.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025285818.1

ORF Insert Sequence:

ATGATTGTGC CGTCGCCGCC AGTTCCAAGC CGGGTCTCCA TCATAGCTCC CTGCACCCTC 
CTCTTCTCTG TGATTTTTAT AGGTCACAAC CACCTCTTTT GTGTGCTAAC TTGTATCCTG 
TGTTTCTGCC TCCCTTCAGA TACATTCCTC GAGGTCAACG TGGTTGTCTG CAGTGTTCTC 
CAGGGCCTGG CACAGGGTAA TCCCAGAGGC CCCAGGGACC TGGGGACTGG CAGAATGCAC 
GACTGGCACA GGATCTTTGA CCAAAACCAG CTGGTCCCTC CGCACCCACA GAGAGCACGC 
CAGCCTCTGA AGGAATCGAC AGCGTTTCAG TGTGTCCTGA AGTGGCTCGA TGGCCCGCTA 
ATTAAGCAGG GGGTGCTGGA GCTGCTATTG GAGGTGGACT GCCACCTGCG CGTGTCCCTC 
TTCGATGTCA CCTATCGGCA CTTCTTTGGG AGGACGTGGA AGAGCTCGCC GAGGCCTGCA 
GAGCCGCTCA CCAGGCAGCC GGCCAGGATC GTCTTCAATG AGCCCTTGTA TTTTCACACG 
TCCTTGAACC ACCCTCATAT TGTGGCCGTG GTGGAGGTGG TCGTTGAAGG CAGGAGACGA 
GACGGGACCC TCCAGACTCT GTCCTGTGGG TTTGGAATTC TTCGGATCTT CAGCCACAAG 
CCGGAATCTC CAACTTCCAC TTCCCAGGAC AAACGGCTGA GACTGTATCA CGGGAGCCCG 
AGAGCCCTCC TCCACCCACT CCTTCAGGAC CCCATAGAAC AGAACAAGCA CATGACCCTC 
ATTGAGAACT GCAGCCTGCA GTACACGCTG AGGCCACACC CGCTTCTGGA GCCCGTGTTC 
CACCTTCTTC CCGAGAACCT GCTGGTGTCC GGTCTGCAGC AGATACCTGG TCTCCTTCCA 
GCTCACGGAG AGTCAGGAGA TGCCCTCCGG AAGCCCCGCC TGCAGAAGCC TCTGACGTGC 
TACCTGGATG ATCTGTTCTT CACGCTGTAT CCCTCGCTGG AGAAGTTCGA GGAGGAACTT 
CTGGATCTTG TGATCAGCGA TTGCTTCCGG GAGGCGGGCA GCCCTCTGGA TGTCGGCGCC 
TTGGAGATCC TGGAGAGGCG CCTGTGCGTG GGCGTGCACA ATGGTTTGGG GTTTGTGCAG 
CGGCCGCAGG TCCTCGTGCT GGTGCCCGAG CTGGAGGTGG CCTTGACACG CTCTGCCAGC 
TTCAGCCGGA AGGTCGGCTC CTCTTCCAAG GCCAGCTCTG GAAACCAGGC GCTGGTCCTG 
AGAAGCCGTC TCCGACTCCC TGAGATGGTC CATCACCCCG CGTTCGCCAT TGTCTTCCAG 
CTGGAGTACG TGTTTAACAG CCCCTCGGGG GCGGACGGCA ATGCAGCTTC ACTCACCTCC 
TTGTCCAGCC TGGCATGCAT GCACATGGTT CGCTGGGCTG TCTGGAACCC CTCGTTGGAA 
GCCAGCTCCG GACAGGTGAT GTTACCTCTC CAGGGTGGCA TCCAGTCCAA CCCTTCGCAC 
TGTCTGGTCT ATAAGGTACC CCCAGCCAGC ATGAGCTCTG AAGAGGTGAA GCAGGTGGAG 
TCAGGGACGG TCCAGTTCCA GTACTCGCTG AGCCCTGAAG AACACCCAGA CACACCCACA 
AGGAATGCAA GCGGCCCCAA GGCAGAGCGG CGGTCTTTTA GGAAGCCACC CGCGTCCCCT 
TCAAGTCCAC CAGCATCGCC GTCCCAGGGG CTCACCGCTC CCCAGGACTC ACCCATGGGA 
CCAGGGCTGT CGCTTTCCCA GCTGGCGGCT TCCCCACCAT CCCCAGCTCC ACGCCGCCGC 
TCGGCCAAAC CCTCATCACA GCCACCTGGG AGCCCTGTGC CCTCCCTGGT CCCAGGGGAG 
TTCCCCTTGG AGGGCAGGAT CTCTCACCTG GAGGCTGACC TGAGCCAGAC AGCCTTAGCC 
CTGGGAACGT CCGCTGCTGA CCACTTGCAG GAACTGCCTT TCACGCCTGT GCACGCCCCG 
ATCATTGCAG GAACCCAGAC CAGGAGCTCT GGAGGGAAGC TATCACGGGC GTCCCTGGCG 
CTGCTGCAGT CTGCTGGCTT TCCCGAGATT CTGGACGCCA ACAAGCAACC AGCTGAGGCT 
GTGAGTCCTA CAGACCCCGT GAAGTTCAGC CCTCAGAAGG AAGAATCAGA CTGTCTACAA 
GGCAATGAGA TAGTGCTGCA ATTTCTTGCC TTTAGCAGGG TGCCCCAGGA TGAGCAGGCA 
GCGCCATGGC CACAGACTGT GTACTTCACC TTCCAATTCT ACCGCTTCCC ACCCGAGACC 
ACACCACGAC TGCGGCTCGT GGAGCTGGCC GATGCTGGGA AAACCAGCTC TGCCTCCCTG 
TCGCACATCC TCGCCCAGAT CCAGAAGGAT GGCTCCTTTG ATGCGGGGTC TCCGGGCTTT 
CAGCGGAAGT ACCTTGTAGA CCCCGGGTTC CTGAGACCCG GGGAGCAGCG CTGGTTCGCC 
CGCTACCTGG CCATGCAGAC CCTGCAGATT GACGTCTGGG ATGGGGACTC CCTGCTTCTC 
GTCGGATCTG CTGCCGTTCA GCTGAAGCAC CTTCTCCGCC AGGGGCGGCC AGCCGTGCAG 
GTCTCGCACG AGCTGGAGGT TGTGGCGACT GAATATGAGC AGGACGCCAT GGTGGTGAGT 
GGAGACATGA CCAAGTTTGG CTGCGTCAGA CCCATCGACG TCCACACAGT GGTGAAGGGC 
CAGCTGCACT TGACATTGGC CAACGTGGCA GTGAAAAATG TGGTCCAAGC CCAGAAGCTG 
GCTGATGTGG ACAGCGAACT GGCCGCCCTG CTCTTCACAC CCCCAAGACC AGGGGCCCGG 
GGGCCCCAGG GCATCAGTCA AGAGGGGGAT GCCGTCCGCA GGCGCAAGCT GGAGCGCATG 
AGGGCCGTGC GGCTGCGGGA GTGTGGATCG GAGCCGGGCC GCCGCGGGCT GGGCGTGCTG 
GCCCAGCAGA GCCTCCATGC CCAGCACGCC CGAGACCTGC AGGTCATTGC CGCCTACCGT 
GAGCGCACGA AGGCGGAGAG CATCGCCAGT GCGCTGAGCA TGGCCATCAC CACGCAGCAC 
ACCATCCACG CCACCCTAGG CACTGCCGAG TTCTTCGAGT TCGCCCTGAG GAACCCCCAC 
AACGCGCAGC ACACGGTGAC CATCGAGATA GACAACCCCG AGCTCAGTCT CATCCTGGAT 
GGTCAGGAGT GGCGGTACTT CAAGGACATG GCTGACTTGC ACACACCCCT GGAGGAGGAC 
ATGTTCCACC TGCGGGGCAG CCTGGCCCCC CAACTCTACC TGCGACCTCG GGAGACTGCC 
CACATCCCCT TCAAGTTCCA GACTTTCTCT GTGGGCCCCT GGGCCTCGGC ACAGGCCTCT 
GCTGAGCTGA AATCAGAGAA GGACACGGAT GCTGGATCAC TCTATAAGTC CAGCATGATG 
CCCACCAAAC ATGCCAAGGT CCTGTTCCGC GCGGGCAGCG GCAAGCCCCT GGCCGTGCTC 
TGCCTGACGG TGGAGCTGCA GCCTCACGTG GTAGACCAGG TCTTCCGCTT CCACCACCCG 
GAGCTCACCT TCCTGAAGAA GGCCATCCGC CTGCCGCCCT GGCACACGCT TCCAGGTGCC 
CCTGTAGGGA CTTCCAGTGA GGACCCCCCT TTCCACGTTC GATGTAGTGA CCCGAACATC 
ATTTGTGAGA CACAGAATGT GGGCCCCGGG GAGCCCCGTG ACGTGTTCCT GAAGGTGGCC 
AGTGGCCCTA GCCCGGAGGT CAAGGACTTC TTTGTCACCA TCTACGTAGA CCGCTGGTTA 
GCAACACCCG TCCAGACCTG GCAGGTCCAC CTGCACTCGC TGCAGCGAGT GGACGTCTCC 
TGTATCACTG GCCAGAGGAC CCGCCTGGCT CTCGTCCTCC GGGGGACGCA GACAGTTAGG 
AGAGTGCAGG CTTTCAGCTC ACACCCCCAG GAGCTGACGA CGGACCCCAG AGGTGTGTTC 
GTGCTGCCTC CTCACGGGGT GCAGGACTTG CATGTGAGCG TCAGGCCCCG CAGGCCGGGC 
AGCCGCTTCG CGCACCTCCA CCTGGTCGAC GTGGACGCCC ACCAGCTGGT GGCCTCGTGG 
CTCCTGTGTC TCTCCTGCCG GCCGCCTCTC ATCTCCAAGG CCTTCGAGAT AAAGCTGCCC 
ACGGGGAGCG GGAGGGGCGC CAACAAGCGC ATCACCTACA CCAACCCCTA CCCCTCGCGG 
AGGGCCTACC GCCTGCACAG CGACCACCCC CACCTGCTGC AGTTCAAGGA GGACGCCTTC 
CAGGTCGGGG GAGGCGAGAC CTATACCATC GGCCTGCGGT TTGCGTCCCG TCAGAGAGCT 
GGGGAGGAAG AGATCCTGAT TTACATCAAC GACCACGAAG ACAAAAACGA GGAGACCTTT 
TGCGTGAAGG TCATCTACCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_025285819.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141604.1 Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X4, mRNA.

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CloneID	OBu66237
Clone ID Related Accession (Same CDS sequence)	XM_025285819.1
Accession Version	XM_025285819.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	nephrocystin-4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGC CGTCGCCGCC AGTTCCAAGC CGGGTCTCCA TCATAGCTCC CTGCACCCTC 
CTCTTCTCTG TGATTTTTAT AGGTCACAAC CACCTCTTTT GTGTGCTAAC TTGTATCCTG 
TGTTTCTGCC TCCCTTCAGA TACATTCCTC GAGGTCAACG TGGTTGTCTG CAGTGTTCTC 
CAGGGCCTGG CACAGGGTAA TCCCAGAGGC CCCAGGGACC TGGGGACTGG CAGAATGCAC 
GACTGGCACA GGATCTTTGA CCAAAACCAG CTGGTCCCTC CGCACCCACA GAGAGCACGC 
CAGCCTCTGA AGGAATCGAC AGCGTTTCAG TGTGTCCTGA AGTGGCTCGA TGGCCCGCTA 
ATTAAGCAGG GGGTGCTGGA GCTGCTATTG GAGGTGGACT GCCACCTGCG CGTGTCCCTC 
TTCGATGTCA CCTATCGGCA CTTCTTTGGG AGGACGTGGA AGAGCTCGCC GAGGCCTGCA 
GAGCCGCTCA CCAGGCAGCC GGCCAGGATC GTCTTCAATG AGCCCTTGTA TTTTCACACG 
TCCTTGAACC ACCCTCATAT TGTGGCCGTG GTGGAGGTGG TCGTTGAAGG CAGGAGACGA 
GACGGGACCC TCCAGACTCT GTCCTGTGGG TTTGGAATTC TTCGGATCTT CAGCCACAAG 
CCGGAATCTC CAACTTCCAC TTCCCAGGAC AAACGGCTGA GACTGTATCA CGGGAGCCCG 
AGAGCCCTCC TCCACCCACT CCTTCAGGAC CCCATAGAAC GAGATGCCCT CCGGAAGCCC 
CGCCTGCAGA AGCCTCTGAC GTGCTACCTG GATGATCTGT TCTTCACGCT GTATCCCTCG 
CTGGAGAAGT TCGAGGAGGA ACTTCTGGAT CTTGTGATCA GCGATTGCTT CCGGGAGGCG 
GGCAGCCCTC TGGATGTCGG CGCCTTGGAG ATCCTGGAGA GGCGCCTGTG CGTGGGCGTG 
CACAATGGTT TGGGGTTTGT GCAGCGGCCG CAGGTCCTCG TGCTGGTGCC CGAGCTGGAG 
GTGGCCTTGA CACGCTCTGC CAGCTTCAGC CGGAAGGTCG GCTCCTCTTC CAAGGCCAGC 
TCTGGAAACC AGGCGCTGGT CCTGAGAAGC CGTCTCCGAC TCCCTGAGAT GGTCCATCAC 
CCCGCGTTCG CCATTGTCTT CCAGCTGGAG TACGTGTTTA ACAGCCCCTC GGGGGCGGAC 
GGCAATGCAG CTTCACTCAC CTCCTTGTCC AGCCTGGCAT GCATGCACAT GGTTCGCTGG 
GCTGTCTGGA ACCCCTCGTT GGAAGCCAGC TCCGGACAGG TGATGTTACC TCTCCAGGGT 
GGCATCCAGT CCAACCCTTC GCACTGTCTG GTCTATAAGG TACCCCCAGC CAGCATGAGC 
TCTGAAGAGG TGAAGCAGGT GGAGTCAGGG ACGGTCCAGT TCCAGTACTC GCTGAGCCCT 
GAAGAACACC CAGACACACC CACAAGGAAT GCAAGCGGCC CCAAGGCAGA GCGGCGGTCT 
TTTAGGAAGC CACCCGCGTC CCCTTCAAGT CCACCAGCAT CGCCGTCCCA GGGGCTCACC 
GCTCCCCAGG ACTCACCCAT GGGACCAGGG CTGTCGCTTT CCCAGCTGGC GGCTTCCCCA 
CCATCCCCAG CTCCACGCCG CCGCTCGGCC AAACCCTCAT CACAGCCACC TGGGAGCCCT 
GTGCCCTCCC TGGTCCCAGG GGAGTTCCCC TTGGAGGGCA GGATCTCTCA CCTGGAGGCT 
GACCTGAGCC AGACAGCCTT AGCCCTGGGA ACGTCCGCTG CTGACCACTT GCAGGAACTG 
CCTTTCACGC CTGTGCACGC CCCGATCATT GCAGGAACCC AGACCAGGAG CTCTGGAGGG 
AAGCTATCAC GGGCGTCCCT GGCGCTGCTG CAGTCTGCTG GCTTTCCCGA GATTCTGGAC 
GCCAACAAGC AACCAGCTGA GGCTGTGAGT CCTACAGACC CCGTGAAGTT CAGCCCTCAG 
AAGGAAGAAT CAGACTGTCT ACAAGGCAAT GAGATAGTGC TGCAATTTCT TGCCTTTAGC 
AGGGTGCCCC AGGATGAGCA GGCAGCGCCA TGGCCACAGA CTGTGTACTT CACCTTCCAA 
TTCTACCGCT TCCCACCCGA GACCACACCA CGACTGCGGC TCGTGGAGCT GGCCGATGCT 
GGGAAAACCA GCTCTGCCTC CCTGTCGCAC ATCCTCGCCC AGATCCAGAA GGATGGCTCC 
TTTGATGCGG GGTCTCCGGG CTTTCAGCGG AAGTACCTTG TAGACCCCGG GTTCCTGAGA 
CCCGGGGAGC AGCGCTGGTT CGCCCGCTAC CTGGCCATGC AGACCCTGCA GATTGACGTC 
TGGGATGGGG ACTCCCTGCT TCTCGTCGGA TCTGCTGCCG TTCAGCTGAA GCACCTTCTC 
CGCCAGGGGC GGCCAGCCGT GCAGGTCTCG CACGAGCTGG AGGTTGTGGC GACTGAATAT 
GAGCAGGACG CCATGGTGGT GAGTGGAGAC ATGACCAAGT TTGGCTGCGT CAGACCCATC 
GACGTCCACA CAGTGGTGAA GGGCCAGCTG CACTTGACAT TGGCCAACGT GGGTCACTTG 
TGTGAACAGA GAGTAAGGAG TCCCAGTTCC CTGCCACCTT CCAGGTCGAG GGTCATCTCA 
AATGATGGAG TCGGCAACTT CATGGGAGGC AGCCTCCTGA CACCCGGGGG CCCCAGATCA 
GTGAAAAATG TGGTCCAAGC CCAGAAGCTG GCTGATGTGG ACAGCGAACT GGCCGCCCTG 
CTCTTCACAC CCCCAAGACC AGGGGCCCGG GGGCCCCAGG GCATCAGTCA AGAGGGGGAT 
GCCGTCCGCA GGCGCAAGCT GGAGCGCATG AGGGCCGTGC GGCTGCGGGA GTGTGGATCG 
GAGCCGGGCC GCCGCGGGCT GGGCGTGCTG GCCCAGCAGA GCCTCCATGC CCAGCACGCC 
CGAGACCTGC AGGTCATTGC CGCCTACCGT GAGCGCACGA AGGCGGAGAG CATCGCCAGT 
GCGCTGAGCA TGGCCATCAC CACGCAGCAC ACCATCCACG CCACCCTAGG CACTGCCGAG 
TTCTTCGAGT TCGCCCTGAG GAACCCCCAC AACGCGCAGC ACACGGTGAC CATCGAGATA 
GACAACCCCG AGCTCAGTCT CATCCTGGAT GGTCAGGAGT GGCGGTACTT CAAGGACATG 
GCTGACTTGC ACACACCCCT GGAGGAGGAC ATGTTCCACC TGCGGGGCAG CCTGGCCCCC 
CAACTCTACC TGCGACCTCG GGAGACTGCC CACATCCCCT TCAAGTTCCA GACTTTCTCT 
GTGGGCCCCT GGGCCTCGGC ACAGGCCTCT GCTGAGCTGA AATCAGAGAA GGACACGGAT 
GCTGGATCAC TCTATAAGTC CAGCATGATG CCCACCAAAC ATGCCAAGGT CCTGTTCCGC 
GCGGGCAGCG GCAAGCCCCT GGCCGTGCTC TGCCTGACGG TGGAGCTGCA GCCTCACGTG 
GTAGACCAGG TCTTCCGCTT CCACCACCCG GAGCTCACCT TCCTGAAGAA GGCCATCCGC 
CTGCCGCCCT GGCACACGCT TCCAGGTGCC CCTGTAGGGA CTTCCAGTGA GGACCCCCCT 
TTCCACGTTC GATGTAGTGA CCCGAACATC ATTTGTGAGA CACAGAATGT GGGCCCCGGG 
GAGCCCCGTG ACGTGTTCCT GAAGGTGGCC AGTGGCCCTA GCCCGGAGGT CAAGGACTTC 
TTTGTCACCA TCTACGTAGA CCGCTGGTTA GCAACACCCG TCCAGACCTG GCAGGTCCAC 
CTGCACTCGC TGCAGCGAGT GGACGTCTCC TGTATCACTG GCCAGAGGAC CCGCCTGGCT 
CTCGTCCTCC GGGGGACGCA GACAGTTAGG AGAGTGCAGG CTTTCAGCTC ACACCCCCAG 
GAGCTGACGA CGGACCCCAG AGGTGTGTTC GTGCTGCCTC CTCACGGGGT GCAGGACTTG 
CATGTGAGCG TCAGGCCCCG CAGGCCGGGC AGCCGCTTCG CGCACCTCCA CCTGGTCGAC 
GTGGACGCCC ACCAGCTGGT GGCCTCGTGG CTCCTGTGTC TCTCCTGCCG GCCGCCTCTC 
ATCTCCAAGG CCTTCGAGAT AAAGCTGCCC ACGGGGAGCG GGAGGGGCGC CAACAAGCGC 
ATCACCTACA CCAACCCCTA CCCCTCGCGG AGGGCCTACC GCCTGCACAG CGACCACCCC 
CACCTGCTGC AGTTCAAGGA GGACGCCTTC CAGGTCGGGG GAGGCGAGAC CTATACCATC 
GGCCTGCGGT TTGCGTCCCG TCAGAGAGCT GGGGAGGAAG AGATCCTGAT TTACATCAAC 
GACCACGAAG ACAAAAACGA GGAGACCTTT TGCGTGAAGG TCATCTACCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141604.1
CDS	41..4414
Translation	MIVPSPPVPSRVSIIAPCTLLFSVIFIGHNHLFCVLTCILCFCLPSDTFLEVNVVVCSVLQGLAQGNPRGPRDLGTGRMHDWHRIFDQNQLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLLEVDCHLRVSLFDVTYRHFFGRTWKSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIERDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLDLVISDCFREAGSPLDVGALEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVHHPAFAIVFQLEYVFNSPSGADGNAASLTSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASMSSEEVKQVESGTVQFQYSLSPEEHPDTPTRNASGPKAERRSFRKPPASPSSPPASPSQGLTAPQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLRLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLVGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTPPRPGARGPQGISQEGDAVRRRKLERMRAVRLRECGSEPGRRGLGVLAQQSLHAQHARDLQVIAAYRERTKAESIASALSMAITTQHTIHATLGTAEFFEFALRNPHNAQHTVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLYLRPRETAHIPFKFQTFSVGPWASAQASAELKSEKDTDAGSLYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQNVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPRRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPTGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFASRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_025285819.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025285819.1

ORF Insert Sequence:

ATGATTGTGC CGTCGCCGCC AGTTCCAAGC CGGGTCTCCA TCATAGCTCC CTGCACCCTC 
CTCTTCTCTG TGATTTTTAT AGGTCACAAC CACCTCTTTT GTGTGCTAAC TTGTATCCTG 
TGTTTCTGCC TCCCTTCAGA TACATTCCTC GAGGTCAACG TGGTTGTCTG CAGTGTTCTC 
CAGGGCCTGG CACAGGGTAA TCCCAGAGGC CCCAGGGACC TGGGGACTGG CAGAATGCAC 
GACTGGCACA GGATCTTTGA CCAAAACCAG CTGGTCCCTC CGCACCCACA GAGAGCACGC 
CAGCCTCTGA AGGAATCGAC AGCGTTTCAG TGTGTCCTGA AGTGGCTCGA TGGCCCGCTA 
ATTAAGCAGG GGGTGCTGGA GCTGCTATTG GAGGTGGACT GCCACCTGCG CGTGTCCCTC 
TTCGATGTCA CCTATCGGCA CTTCTTTGGG AGGACGTGGA AGAGCTCGCC GAGGCCTGCA 
GAGCCGCTCA CCAGGCAGCC GGCCAGGATC GTCTTCAATG AGCCCTTGTA TTTTCACACG 
TCCTTGAACC ACCCTCATAT TGTGGCCGTG GTGGAGGTGG TCGTTGAAGG CAGGAGACGA 
GACGGGACCC TCCAGACTCT GTCCTGTGGG TTTGGAATTC TTCGGATCTT CAGCCACAAG 
CCGGAATCTC CAACTTCCAC TTCCCAGGAC AAACGGCTGA GACTGTATCA CGGGAGCCCG 
AGAGCCCTCC TCCACCCACT CCTTCAGGAC CCCATAGAAC GAGATGCCCT CCGGAAGCCC 
CGCCTGCAGA AGCCTCTGAC GTGCTACCTG GATGATCTGT TCTTCACGCT GTATCCCTCG 
CTGGAGAAGT TCGAGGAGGA ACTTCTGGAT CTTGTGATCA GCGATTGCTT CCGGGAGGCG 
GGCAGCCCTC TGGATGTCGG CGCCTTGGAG ATCCTGGAGA GGCGCCTGTG CGTGGGCGTG 
CACAATGGTT TGGGGTTTGT GCAGCGGCCG CAGGTCCTCG TGCTGGTGCC CGAGCTGGAG 
GTGGCCTTGA CACGCTCTGC CAGCTTCAGC CGGAAGGTCG GCTCCTCTTC CAAGGCCAGC 
TCTGGAAACC AGGCGCTGGT CCTGAGAAGC CGTCTCCGAC TCCCTGAGAT GGTCCATCAC 
CCCGCGTTCG CCATTGTCTT CCAGCTGGAG TACGTGTTTA ACAGCCCCTC GGGGGCGGAC 
GGCAATGCAG CTTCACTCAC CTCCTTGTCC AGCCTGGCAT GCATGCACAT GGTTCGCTGG 
GCTGTCTGGA ACCCCTCGTT GGAAGCCAGC TCCGGACAGG TGATGTTACC TCTCCAGGGT 
GGCATCCAGT CCAACCCTTC GCACTGTCTG GTCTATAAGG TACCCCCAGC CAGCATGAGC 
TCTGAAGAGG TGAAGCAGGT GGAGTCAGGG ACGGTCCAGT TCCAGTACTC GCTGAGCCCT 
GAAGAACACC CAGACACACC CACAAGGAAT GCAAGCGGCC CCAAGGCAGA GCGGCGGTCT 
TTTAGGAAGC CACCCGCGTC CCCTTCAAGT CCACCAGCAT CGCCGTCCCA GGGGCTCACC 
GCTCCCCAGG ACTCACCCAT GGGACCAGGG CTGTCGCTTT CCCAGCTGGC GGCTTCCCCA 
CCATCCCCAG CTCCACGCCG CCGCTCGGCC AAACCCTCAT CACAGCCACC TGGGAGCCCT 
GTGCCCTCCC TGGTCCCAGG GGAGTTCCCC TTGGAGGGCA GGATCTCTCA CCTGGAGGCT 
GACCTGAGCC AGACAGCCTT AGCCCTGGGA ACGTCCGCTG CTGACCACTT GCAGGAACTG 
CCTTTCACGC CTGTGCACGC CCCGATCATT GCAGGAACCC AGACCAGGAG CTCTGGAGGG 
AAGCTATCAC GGGCGTCCCT GGCGCTGCTG CAGTCTGCTG GCTTTCCCGA GATTCTGGAC 
GCCAACAAGC AACCAGCTGA GGCTGTGAGT CCTACAGACC CCGTGAAGTT CAGCCCTCAG 
AAGGAAGAAT CAGACTGTCT ACAAGGCAAT GAGATAGTGC TGCAATTTCT TGCCTTTAGC 
AGGGTGCCCC AGGATGAGCA GGCAGCGCCA TGGCCACAGA CTGTGTACTT CACCTTCCAA 
TTCTACCGCT TCCCACCCGA GACCACACCA CGACTGCGGC TCGTGGAGCT GGCCGATGCT 
GGGAAAACCA GCTCTGCCTC CCTGTCGCAC ATCCTCGCCC AGATCCAGAA GGATGGCTCC 
TTTGATGCGG GGTCTCCGGG CTTTCAGCGG AAGTACCTTG TAGACCCCGG GTTCCTGAGA 
CCCGGGGAGC AGCGCTGGTT CGCCCGCTAC CTGGCCATGC AGACCCTGCA GATTGACGTC 
TGGGATGGGG ACTCCCTGCT TCTCGTCGGA TCTGCTGCCG TTCAGCTGAA GCACCTTCTC 
CGCCAGGGGC GGCCAGCCGT GCAGGTCTCG CACGAGCTGG AGGTTGTGGC GACTGAATAT 
GAGCAGGACG CCATGGTGGT GAGTGGAGAC ATGACCAAGT TTGGCTGCGT CAGACCCATC 
GACGTCCACA CAGTGGTGAA GGGCCAGCTG CACTTGACAT TGGCCAACGT GGGTCACTTG 
TGTGAACAGA GAGTAAGGAG TCCCAGTTCC CTGCCACCTT CCAGGTCGAG GGTCATCTCA 
AATGATGGAG TCGGCAACTT CATGGGAGGC AGCCTCCTGA CACCCGGGGG CCCCAGATCA 
GTGAAAAATG TGGTCCAAGC CCAGAAGCTG GCTGATGTGG ACAGCGAACT GGCCGCCCTG 
CTCTTCACAC CCCCAAGACC AGGGGCCCGG GGGCCCCAGG GCATCAGTCA AGAGGGGGAT 
GCCGTCCGCA GGCGCAAGCT GGAGCGCATG AGGGCCGTGC GGCTGCGGGA GTGTGGATCG 
GAGCCGGGCC GCCGCGGGCT GGGCGTGCTG GCCCAGCAGA GCCTCCATGC CCAGCACGCC 
CGAGACCTGC AGGTCATTGC CGCCTACCGT GAGCGCACGA AGGCGGAGAG CATCGCCAGT 
GCGCTGAGCA TGGCCATCAC CACGCAGCAC ACCATCCACG CCACCCTAGG CACTGCCGAG 
TTCTTCGAGT TCGCCCTGAG GAACCCCCAC AACGCGCAGC ACACGGTGAC CATCGAGATA 
GACAACCCCG AGCTCAGTCT CATCCTGGAT GGTCAGGAGT GGCGGTACTT CAAGGACATG 
GCTGACTTGC ACACACCCCT GGAGGAGGAC ATGTTCCACC TGCGGGGCAG CCTGGCCCCC 
CAACTCTACC TGCGACCTCG GGAGACTGCC CACATCCCCT TCAAGTTCCA GACTTTCTCT 
GTGGGCCCCT GGGCCTCGGC ACAGGCCTCT GCTGAGCTGA AATCAGAGAA GGACACGGAT 
GCTGGATCAC TCTATAAGTC CAGCATGATG CCCACCAAAC ATGCCAAGGT CCTGTTCCGC 
GCGGGCAGCG GCAAGCCCCT GGCCGTGCTC TGCCTGACGG TGGAGCTGCA GCCTCACGTG 
GTAGACCAGG TCTTCCGCTT CCACCACCCG GAGCTCACCT TCCTGAAGAA GGCCATCCGC 
CTGCCGCCCT GGCACACGCT TCCAGGTGCC CCTGTAGGGA CTTCCAGTGA GGACCCCCCT 
TTCCACGTTC GATGTAGTGA CCCGAACATC ATTTGTGAGA CACAGAATGT GGGCCCCGGG 
GAGCCCCGTG ACGTGTTCCT GAAGGTGGCC AGTGGCCCTA GCCCGGAGGT CAAGGACTTC 
TTTGTCACCA TCTACGTAGA CCGCTGGTTA GCAACACCCG TCCAGACCTG GCAGGTCCAC 
CTGCACTCGC TGCAGCGAGT GGACGTCTCC TGTATCACTG GCCAGAGGAC CCGCCTGGCT 
CTCGTCCTCC GGGGGACGCA GACAGTTAGG AGAGTGCAGG CTTTCAGCTC ACACCCCCAG 
GAGCTGACGA CGGACCCCAG AGGTGTGTTC GTGCTGCCTC CTCACGGGGT GCAGGACTTG 
CATGTGAGCG TCAGGCCCCG CAGGCCGGGC AGCCGCTTCG CGCACCTCCA CCTGGTCGAC 
GTGGACGCCC ACCAGCTGGT GGCCTCGTGG CTCCTGTGTC TCTCCTGCCG GCCGCCTCTC 
ATCTCCAAGG CCTTCGAGAT AAAGCTGCCC ACGGGGAGCG GGAGGGGCGC CAACAAGCGC 
ATCACCTACA CCAACCCCTA CCCCTCGCGG AGGGCCTACC GCCTGCACAG CGACCACCCC 
CACCTGCTGC AGTTCAAGGA GGACGCCTTC CAGGTCGGGG GAGGCGAGAC CTATACCATC 
GGCCTGCGGT TTGCGTCCCG TCAGAGAGCT GGGGAGGAAG AGATCCTGAT TTACATCAAC 
GACCACGAAG ACAAAAACGA GGAGACCTTT TGCGTGAAGG TCATCTACCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_025285820.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141605.1 Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X5, mRNA.

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CloneID	OBu66238
Clone ID Related Accession (Same CDS sequence)	XM_025285820.1
Accession Version	XM_025285820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	nephrocystin-4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGC CGTCGCCGCC AGTTCCAAGC CGGGTCTCCA TCATAGCTCC CTGCACCCTC 
CTCTTCTCTG TGATTTTTAT AGGTCACAAC CACCTCTTTT GTGTGCTAAC TTGTATCCTG 
TGTTTCTGCC TCCCTTCAGA TACATTCCTC GAGGTCAACG TGGTTGTCTG CAGTGTTCTC 
CAGGGCCTGG CACAGGGTAA TCCCAGAGGC CCCAGGGACC TGGGGACTGG CAGAATGCAC 
GACTGGCACA GGATCTTTGA CCAAAACCAG CTGGTCCCTC CGCACCCACA GAGAGCACGC 
CAGCCTCTGA AGGAATCGAC AGCGTTTCAG TGTGTCCTGA AGTGGCTCGA TGGCCCGCTA 
ATTAAGCAGG GGGTGCTGGA GCTGCTATTG GAGGTGGACT GCCACCTGCG CGTGTCCCTC 
TTCGATGTCA CCTATCGGCA CTTCTTTGGG AGGACGTGGA AGAGCTCGCC GAGGCCTGCA 
GAGCCGCTCA CCAGGCAGCC GGCCAGGATC GTCTTCAATG AGCCCTTGTA TTTTCACACG 
TCCTTGAACC ACCCTCATAT TGTGGCCGTG GTGGAGGTGG TCGTTGAAGG CAGGAGACGA 
GACGGGACCC TCCAGACTCT GTCCTGTGGG TTTGGAATTC TTCGGATCTT CAGCCACAAG 
CCGGAATCTC CAACTTCCAC TTCCCAGGAC AAACGGCTGA GACTGTATCA CGGGAGCCCG 
AGAGCCCTCC TCCACCCACT CCTTCAGGAC CCCATAGAAC AGAACAAGCA CATGACCCTC 
ATTGAGAACT GCAGCCTGCA GTACACGCTG AGGCCACACC CGCTTCTGGA GCCCGTGTTC 
CACCTTCTTC CCGAGAACCT GCTGGTGTCC GGTCTGCAGC AGATACCTGG TCTCCTTCCA 
GCTCACGGAG AGTCAGGAGA TGCCCTCCGG AAGCCCCGCC TGCAGAAGCC TCTGACGTGC 
TACCTGGATG ATCTGTTCTT CACGCTGTAT CCCTCGCTGG AGAAGTTCGA GGAGGAACTT 
CTGGATCTTG TGATCAGCGA TTGCTTCCGG GAGGCGGGCA GCCCTCTGGA TGTCGGCGCC 
TTGGAGATCC TGGAGAGGCG CCTGTGCGTG GGCGTGCACA ATGGTTTGGG GTTTGTGCAG 
CGGCCGCAGG TCCTCGTGCT GGTGCCCGAG CTGGAGGTGG CCTTGACACG CTCTGCCAGC 
TTCAGCCGGA AGGTCGGCTC CTCTTCCAAG GCCAGCTCTG GAAACCAGGC GCTGGTCCTG 
AGAAGCCGTC TCCGACTCCC TGAGATGGTC CATCACCCCG CGTTCGCCAT TGTCTTCCAG 
CTGGAGTACG TGTTTAACAG CCCCTCGGGG GCGGACGGCA ATGTGAAGCA GGTGGAGTCA 
GGGACGGTCC AGTTCCAGTA CTCGCTGAGC CCTGAAGAAC ACCCAGACAC ACCCACAAGG 
AATGCAAGCG GCCCCAAGGC AGAGCGGCGG TCTTTTAGGA AGCCACCCGC GTCCCCTTCA 
AGTCCACCAG CATCGCCGTC CCAGGGGCTC ACCGCTCCCC AGGACTCACC CATGGGACCA 
GGGCTGTCGC TTTCCCAGCT GGCGGCTTCC CCACCATCCC CAGCTCCACG CCGCCGCTCG 
GCCAAACCCT CATCACAGCC ACCTGGGAGC CCTGTGCCCT CCCTGGTCCC AGGGGAGTTC 
CCCTTGGAGG GCAGGATCTC TCACCTGGAG GCTGACCTGA GCCAGACAGC CTTAGCCCTG 
GGAACGTCCG CTGCTGACCA CTTGCAGGAA CTGCCTTTCA CGCCTGTGCA CGCCCCGATC 
ATTGCAGGAA CCCAGACCAG GAGCTCTGGA GGGAAGCTAT CACGGGCGTC CCTGGCGCTG 
CTGCAGTCTG CTGGCTTTCC CGAGATTCTG GACGCCAACA AGCAACCAGC TGAGGCTGTG 
AGTCCTACAG ACCCCGTGAA GTTCAGCCCT CAGAAGGAAG AATCAGACTG TCTACAAGGC 
AATGAGATAG TGCTGCAATT TCTTGCCTTT AGCAGGGTGC CCCAGGATGA GCAGGCAGCG 
CCATGGCCAC AGACTGTGTA CTTCACCTTC CAATTCTACC GCTTCCCACC CGAGACCACA 
CCACGACTGC GGCTCGTGGA GCTGGCCGAT GCTGGGAAAA CCAGCTCTGC CTCCCTGTCG 
CACATCCTCG CCCAGATCCA GAAGGATGGC TCCTTTGATG CGGGGTCTCC GGGCTTTCAG 
CGGAAGTACC TTGTAGACCC CGGGTTCCTG AGACCCGGGG AGCAGCGCTG GTTCGCCCGC 
TACCTGGCCA TGCAGACCCT GCAGATTGAC GTCTGGGATG GGGACTCCCT GCTTCTCGTC 
GGATCTGCTG CCGTTCAGCT GAAGCACCTT CTCCGCCAGG GGCGGCCAGC CGTGCAGGTC 
TCGCACGAGC TGGAGGTTGT GGCGACTGAA TATGAGCAGG ACGCCATGGT GGTGAGTGGA 
GACATGACCA AGTTTGGCTG CGTCAGACCC ATCGACGTCC ACACAGTGGT GAAGGGCCAG 
CTGCACTTGA CATTGGCCAA CGTGGGTCAC TTGTGTGAAC AGAGAGTAAG GAGTCCCAGT 
TCCCTGCCAC CTTCCAGGTC GAGGGTCATC TCAAATGATG GAGTCGGCAA CTTCATGGGA 
GGCAGCCTCC TGACACCCGG GGGCCCCAGA TCAGTGAAAA ATGTGGTCCA AGCCCAGAAG 
CTGGCTGATG TGGACAGCGA ACTGGCCGCC CTGCTCTTCA CACCCCCAAG ACCAGGGGCC 
CGGGGGCCCC AGGGCATCAG TCAAGAGGGG GATGCCGTCC GCAGGCGCAA GCTGGAGCGC 
ATGAGGGCCG TGCGGCTGCG GGAGTGTGGA TCGGAGCCGG GCCGCCGCGG GCTGGGCGTG 
CTGGCCCAGC AGAGCCTCCA TGCCCAGCAC GCCCGAGACC TGCAGGTCAT TGCCGCCTAC 
CGTGAGCGCA CGAAGGCGGA GAGCATCGCC AGTGCGCTGA GCATGGCCAT CACCACGCAG 
CACACCATCC ACGCCACCCT AGGCACTGCC GAGTTCTTCG AGTTCGCCCT GAGGAACCCC 
CACAACGCGC AGCACACGGT GACCATCGAG ATAGACAACC CCGAGCTCAG TCTCATCCTG 
GATGGTCAGG AGTGGCGGTA CTTCAAGGAC ATGGCTGACT TGCACACACC CCTGGAGGAG 
GACATGTTCC ACCTGCGGGG CAGCCTGGCC CCCCAACTCT ACCTGCGACC TCGGGAGACT 
GCCCACATCC CCTTCAAGTT CCAGACTTTC TCTGTGGGCC CCTGGGCCTC GGCACAGGCC 
TCTGCTGAGC TGAAATCAGA GAAGGACACG GATGCTGGAT CACTCTATAA GTCCAGCATG 
ATGCCCACCA AACATGCCAA GGTCCTGTTC CGCGCGGGCA GCGGCAAGCC CCTGGCCGTG 
CTCTGCCTGA CGGTGGAGCT GCAGCCTCAC GTGGTAGACC AGGTCTTCCG CTTCCACCAC 
CCGGAGCTCA CCTTCCTGAA GAAGGCCATC CGCCTGCCGC CCTGGCACAC GCTTCCAGGT 
GCCCCTGTAG GGACTTCCAG TGAGGACCCC CCTTTCCACG TTCGATGTAG TGACCCGAAC 
ATCATTTGTG AGACACAGAA TGTGGGCCCC GGGGAGCCCC GTGACGTGTT CCTGAAGGTG 
GCCAGTGGCC CTAGCCCGGA GGTCAAGGAC TTCTTTGTCA CCATCTACGT AGACCGCTGG 
TTAGCAACAC CCGTCCAGAC CTGGCAGGTC CACCTGCACT CGCTGCAGCG AGTGGACGTC 
TCCTGTATCA CTGGCCAGAG GACCCGCCTG GCTCTCGTCC TCCGGGGGAC GCAGACAGTT 
AGGAGAGTGC AGGCTTTCAG CTCACACCCC CAGGAGCTGA CGACGGACCC CAGAGGTGTG 
TTCGTGCTGC CTCCTCACGG GGTGCAGGAC TTGCATGTGA GCGTCAGGCC CCGCAGGCCG 
GGCAGCCGCT TCGCGCACCT CCACCTGGTC GACGTGGACG CCCACCAGCT GGTGGCCTCG 
TGGCTCCTGT GTCTCTCCTG CCGGCCGCCT CTCATCTCCA AGGCCTTCGA GATAAAGCTG 
CCCACGGGGA GCGGGAGGGG CGCCAACAAG CGCATCACCT ACACCAACCC CTACCCCTCG 
CGGAGGGCCT ACCGCCTGCA CAGCGACCAC CCCCACCTGC TGCAGTTCAA GGAGGACGCC 
TTCCAGGTCG GGGGAGGCGA GACCTATACC ATCGGCCTGC GGTTTGCGTC CCGTCAGAGA 
GCTGGGGAGG AAGAGATCCT GATTTACATC AACGACCACG AAGACAAAAA CGAGGAGACC 
TTTTGCGTGA AGGTCATCTA CCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141605.1
CDS	41..4387
Translation	MIVPSPPVPSRVSIIAPCTLLFSVIFIGHNHLFCVLTCILCFCLPSDTFLEVNVVVCSVLQGLAQGNPRGPRDLGTGRMHDWHRIFDQNQLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLLEVDCHLRVSLFDVTYRHFFGRTWKSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLDLVISDCFREAGSPLDVGALEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVHHPAFAIVFQLEYVFNSPSGADGNVKQVESGTVQFQYSLSPEEHPDTPTRNASGPKAERRSFRKPPASPSSPPASPSQGLTAPQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLRLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLVGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTPPRPGARGPQGISQEGDAVRRRKLERMRAVRLRECGSEPGRRGLGVLAQQSLHAQHARDLQVIAAYRERTKAESIASALSMAITTQHTIHATLGTAEFFEFALRNPHNAQHTVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLYLRPRETAHIPFKFQTFSVGPWASAQASAELKSEKDTDAGSLYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQNVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPRRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPTGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFASRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_025285820.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025285820.1

ORF Insert Sequence:

ATGATTGTGC CGTCGCCGCC AGTTCCAAGC CGGGTCTCCA TCATAGCTCC CTGCACCCTC 
CTCTTCTCTG TGATTTTTAT AGGTCACAAC CACCTCTTTT GTGTGCTAAC TTGTATCCTG 
TGTTTCTGCC TCCCTTCAGA TACATTCCTC GAGGTCAACG TGGTTGTCTG CAGTGTTCTC 
CAGGGCCTGG CACAGGGTAA TCCCAGAGGC CCCAGGGACC TGGGGACTGG CAGAATGCAC 
GACTGGCACA GGATCTTTGA CCAAAACCAG CTGGTCCCTC CGCACCCACA GAGAGCACGC 
CAGCCTCTGA AGGAATCGAC AGCGTTTCAG TGTGTCCTGA AGTGGCTCGA TGGCCCGCTA 
ATTAAGCAGG GGGTGCTGGA GCTGCTATTG GAGGTGGACT GCCACCTGCG CGTGTCCCTC 
TTCGATGTCA CCTATCGGCA CTTCTTTGGG AGGACGTGGA AGAGCTCGCC GAGGCCTGCA 
GAGCCGCTCA CCAGGCAGCC GGCCAGGATC GTCTTCAATG AGCCCTTGTA TTTTCACACG 
TCCTTGAACC ACCCTCATAT TGTGGCCGTG GTGGAGGTGG TCGTTGAAGG CAGGAGACGA 
GACGGGACCC TCCAGACTCT GTCCTGTGGG TTTGGAATTC TTCGGATCTT CAGCCACAAG 
CCGGAATCTC CAACTTCCAC TTCCCAGGAC AAACGGCTGA GACTGTATCA CGGGAGCCCG 
AGAGCCCTCC TCCACCCACT CCTTCAGGAC CCCATAGAAC AGAACAAGCA CATGACCCTC 
ATTGAGAACT GCAGCCTGCA GTACACGCTG AGGCCACACC CGCTTCTGGA GCCCGTGTTC 
CACCTTCTTC CCGAGAACCT GCTGGTGTCC GGTCTGCAGC AGATACCTGG TCTCCTTCCA 
GCTCACGGAG AGTCAGGAGA TGCCCTCCGG AAGCCCCGCC TGCAGAAGCC TCTGACGTGC 
TACCTGGATG ATCTGTTCTT CACGCTGTAT CCCTCGCTGG AGAAGTTCGA GGAGGAACTT 
CTGGATCTTG TGATCAGCGA TTGCTTCCGG GAGGCGGGCA GCCCTCTGGA TGTCGGCGCC 
TTGGAGATCC TGGAGAGGCG CCTGTGCGTG GGCGTGCACA ATGGTTTGGG GTTTGTGCAG 
CGGCCGCAGG TCCTCGTGCT GGTGCCCGAG CTGGAGGTGG CCTTGACACG CTCTGCCAGC 
TTCAGCCGGA AGGTCGGCTC CTCTTCCAAG GCCAGCTCTG GAAACCAGGC GCTGGTCCTG 
AGAAGCCGTC TCCGACTCCC TGAGATGGTC CATCACCCCG CGTTCGCCAT TGTCTTCCAG 
CTGGAGTACG TGTTTAACAG CCCCTCGGGG GCGGACGGCA ATGTGAAGCA GGTGGAGTCA 
GGGACGGTCC AGTTCCAGTA CTCGCTGAGC CCTGAAGAAC ACCCAGACAC ACCCACAAGG 
AATGCAAGCG GCCCCAAGGC AGAGCGGCGG TCTTTTAGGA AGCCACCCGC GTCCCCTTCA 
AGTCCACCAG CATCGCCGTC CCAGGGGCTC ACCGCTCCCC AGGACTCACC CATGGGACCA 
GGGCTGTCGC TTTCCCAGCT GGCGGCTTCC CCACCATCCC CAGCTCCACG CCGCCGCTCG 
GCCAAACCCT CATCACAGCC ACCTGGGAGC CCTGTGCCCT CCCTGGTCCC AGGGGAGTTC 
CCCTTGGAGG GCAGGATCTC TCACCTGGAG GCTGACCTGA GCCAGACAGC CTTAGCCCTG 
GGAACGTCCG CTGCTGACCA CTTGCAGGAA CTGCCTTTCA CGCCTGTGCA CGCCCCGATC 
ATTGCAGGAA CCCAGACCAG GAGCTCTGGA GGGAAGCTAT CACGGGCGTC CCTGGCGCTG 
CTGCAGTCTG CTGGCTTTCC CGAGATTCTG GACGCCAACA AGCAACCAGC TGAGGCTGTG 
AGTCCTACAG ACCCCGTGAA GTTCAGCCCT CAGAAGGAAG AATCAGACTG TCTACAAGGC 
AATGAGATAG TGCTGCAATT TCTTGCCTTT AGCAGGGTGC CCCAGGATGA GCAGGCAGCG 
CCATGGCCAC AGACTGTGTA CTTCACCTTC CAATTCTACC GCTTCCCACC CGAGACCACA 
CCACGACTGC GGCTCGTGGA GCTGGCCGAT GCTGGGAAAA CCAGCTCTGC CTCCCTGTCG 
CACATCCTCG CCCAGATCCA GAAGGATGGC TCCTTTGATG CGGGGTCTCC GGGCTTTCAG 
CGGAAGTACC TTGTAGACCC CGGGTTCCTG AGACCCGGGG AGCAGCGCTG GTTCGCCCGC 
TACCTGGCCA TGCAGACCCT GCAGATTGAC GTCTGGGATG GGGACTCCCT GCTTCTCGTC 
GGATCTGCTG CCGTTCAGCT GAAGCACCTT CTCCGCCAGG GGCGGCCAGC CGTGCAGGTC 
TCGCACGAGC TGGAGGTTGT GGCGACTGAA TATGAGCAGG ACGCCATGGT GGTGAGTGGA 
GACATGACCA AGTTTGGCTG CGTCAGACCC ATCGACGTCC ACACAGTGGT GAAGGGCCAG 
CTGCACTTGA CATTGGCCAA CGTGGGTCAC TTGTGTGAAC AGAGAGTAAG GAGTCCCAGT 
TCCCTGCCAC CTTCCAGGTC GAGGGTCATC TCAAATGATG GAGTCGGCAA CTTCATGGGA 
GGCAGCCTCC TGACACCCGG GGGCCCCAGA TCAGTGAAAA ATGTGGTCCA AGCCCAGAAG 
CTGGCTGATG TGGACAGCGA ACTGGCCGCC CTGCTCTTCA CACCCCCAAG ACCAGGGGCC 
CGGGGGCCCC AGGGCATCAG TCAAGAGGGG GATGCCGTCC GCAGGCGCAA GCTGGAGCGC 
ATGAGGGCCG TGCGGCTGCG GGAGTGTGGA TCGGAGCCGG GCCGCCGCGG GCTGGGCGTG 
CTGGCCCAGC AGAGCCTCCA TGCCCAGCAC GCCCGAGACC TGCAGGTCAT TGCCGCCTAC 
CGTGAGCGCA CGAAGGCGGA GAGCATCGCC AGTGCGCTGA GCATGGCCAT CACCACGCAG 
CACACCATCC ACGCCACCCT AGGCACTGCC GAGTTCTTCG AGTTCGCCCT GAGGAACCCC 
CACAACGCGC AGCACACGGT GACCATCGAG ATAGACAACC CCGAGCTCAG TCTCATCCTG 
GATGGTCAGG AGTGGCGGTA CTTCAAGGAC ATGGCTGACT TGCACACACC CCTGGAGGAG 
GACATGTTCC ACCTGCGGGG CAGCCTGGCC CCCCAACTCT ACCTGCGACC TCGGGAGACT 
GCCCACATCC CCTTCAAGTT CCAGACTTTC TCTGTGGGCC CCTGGGCCTC GGCACAGGCC 
TCTGCTGAGC TGAAATCAGA GAAGGACACG GATGCTGGAT CACTCTATAA GTCCAGCATG 
ATGCCCACCA AACATGCCAA GGTCCTGTTC CGCGCGGGCA GCGGCAAGCC CCTGGCCGTG 
CTCTGCCTGA CGGTGGAGCT GCAGCCTCAC GTGGTAGACC AGGTCTTCCG CTTCCACCAC 
CCGGAGCTCA CCTTCCTGAA GAAGGCCATC CGCCTGCCGC CCTGGCACAC GCTTCCAGGT 
GCCCCTGTAG GGACTTCCAG TGAGGACCCC CCTTTCCACG TTCGATGTAG TGACCCGAAC 
ATCATTTGTG AGACACAGAA TGTGGGCCCC GGGGAGCCCC GTGACGTGTT CCTGAAGGTG 
GCCAGTGGCC CTAGCCCGGA GGTCAAGGAC TTCTTTGTCA CCATCTACGT AGACCGCTGG 
TTAGCAACAC CCGTCCAGAC CTGGCAGGTC CACCTGCACT CGCTGCAGCG AGTGGACGTC 
TCCTGTATCA CTGGCCAGAG GACCCGCCTG GCTCTCGTCC TCCGGGGGAC GCAGACAGTT 
AGGAGAGTGC AGGCTTTCAG CTCACACCCC CAGGAGCTGA CGACGGACCC CAGAGGTGTG 
TTCGTGCTGC CTCCTCACGG GGTGCAGGAC TTGCATGTGA GCGTCAGGCC CCGCAGGCCG 
GGCAGCCGCT TCGCGCACCT CCACCTGGTC GACGTGGACG CCCACCAGCT GGTGGCCTCG 
TGGCTCCTGT GTCTCTCCTG CCGGCCGCCT CTCATCTCCA AGGCCTTCGA GATAAAGCTG 
CCCACGGGGA GCGGGAGGGG CGCCAACAAG CGCATCACCT ACACCAACCC CTACCCCTCG 
CGGAGGGCCT ACCGCCTGCA CAGCGACCAC CCCCACCTGC TGCAGTTCAA GGAGGACGCC 
TTCCAGGTCG GGGGAGGCGA GACCTATACC ATCGGCCTGC GGTTTGCGTC CCGTCAGAGA 
GCTGGGGAGG AAGAGATCCT GATTTACATC AACGACCACG AAGACAAAAA CGAGGAGACC 
TTTTGCGTGA AGGTCATCTA CCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_006069602.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006069664.2 Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X6, mRNA.

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CloneID	OBu66239
Clone ID Related Accession (Same CDS sequence)	XM_006069602.2 , XM_006069603.2 , XM_006069604.2
Accession Version	XM_006069602.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	nephrocystin-4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006069602.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCAGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACAGCG TTTCAGTGTG TCCTGAAGTG GCTCGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATTGGAGG TGGACTGCCA CCTGCGCGTG 
TCCCTCTTCG ATGTCACCTA TCGGCACTTC TTTGGGAGGA CGTGGAAGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGG 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCACGGG 
AGCCCGAGAG CCCTCCTCCA CCCACTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT TCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACCTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGTCTC 
CTTCCAGCTC ACGGAGAGTC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAACTTCTGG ATCTTGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC TCTGGATGTC 
GGCGCCTTGG AGATCCTGGA GAGGCGCCTG TGCGTGGGCG TGCACAATGG TTTGGGGTTT 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCCTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCATC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCGG ACGGCAATGC AGCTTCACTC 
ACCTCCTTGT CCAGCCTGGC ATGCATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
TTGGAAGCCA GCTCCGGACA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATGA GCTCTGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGACACA 
CCCACAAGGA ATGCAAGCGG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGCG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTCCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCGCT TTCCCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCACGC 
CGCCGCTCGG CCAAACCCTC ATCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC GCCTGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTATC ACGGGCGTCC 
CTGGCGCTGC TGCAGTCTGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GCTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACAC CACGACTGCG GCTCGTGGAG CTGGCCGATG CTGGGAAAAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT TGTAGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATTGACG TCTGGGATGG GGACTCCCTG 
CTTCTCGTCG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCAGCC 
GTGCAGGTCT CGCACGAGCT GGAGGTTGTG GCGACTGAAT ATGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACAGTGGTG 
AAGGGCCAGC TGCACTTGAC ATTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTCGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCCCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACCCCCAAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAGT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAGTGTGGAT CGGAGCCGGG CCGCCGCGGG 
CTGGGCGTGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC GAAGGCGGAG AGCATCGCCA GTGCGCTGAG CATGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCACTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACACGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTA CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAAATCAGAG AAGGACACGG ATGCTGGATC ACTCTATAAG 
TCCAGCATGA TGCCCACCAA ACATGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTAGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAGGCCATCC GCCTGCCGCC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCACGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAAT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCGGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC CGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
CGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTCG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCACGGGGAG CGGGAGGGGC GCCAACAAGC GCATCACCTA CACCAACCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGCGAG ACCTATACCA TCGGCCTGCG GTTTGCGTCC 
CGTCAGAGAG CTGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006069664.2
CDS	289..4584
Translation	MHDWHRIFDQNQLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLLEVDCHLRVSLFDVTYRHFFGRTWKSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLDLVISDCFREAGSPLDVGALEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVHHPAFAIVFQLEYVFNSPSGADGNAASLTSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASMSSEEVKQVESGTVQFQYSLSPEEHPDTPTRNASGPKAERRSFRKPPASPSSPPASPSQGLTAPQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLRLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLVGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTPPRPGARGPQGISQEGDAVRRRKLERMRAVRLRECGSEPGRRGLGVLAQQSLHAQHARDLQVIAAYRERTKAESIASALSMAITTQHTIHATLGTAEFFEFALRNPHNAQHTVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLYLRPRETAHIPFKFQTFSVGPWASAQASAELKSEKDTDAGSLYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQNVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPRRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPTGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFASRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_006069602.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006069602.2

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCAGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACAGCG TTTCAGTGTG TCCTGAAGTG GCTCGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATTGGAGG TGGACTGCCA CCTGCGCGTG 
TCCCTCTTCG ATGTCACCTA TCGGCACTTC TTTGGGAGGA CGTGGAAGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGG 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCACGGG 
AGCCCGAGAG CCCTCCTCCA CCCACTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT TCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACCTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGTCTC 
CTTCCAGCTC ACGGAGAGTC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAACTTCTGG ATCTTGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC TCTGGATGTC 
GGCGCCTTGG AGATCCTGGA GAGGCGCCTG TGCGTGGGCG TGCACAATGG TTTGGGGTTT 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCCTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCATC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCGG ACGGCAATGC AGCTTCACTC 
ACCTCCTTGT CCAGCCTGGC ATGCATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
TTGGAAGCCA GCTCCGGACA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATGA GCTCTGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGACACA 
CCCACAAGGA ATGCAAGCGG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGCG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTCCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCGCT TTCCCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCACGC 
CGCCGCTCGG CCAAACCCTC ATCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC GCCTGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTATC ACGGGCGTCC 
CTGGCGCTGC TGCAGTCTGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GCTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACAC CACGACTGCG GCTCGTGGAG CTGGCCGATG CTGGGAAAAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT TGTAGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATTGACG TCTGGGATGG GGACTCCCTG 
CTTCTCGTCG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCAGCC 
GTGCAGGTCT CGCACGAGCT GGAGGTTGTG GCGACTGAAT ATGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACAGTGGTG 
AAGGGCCAGC TGCACTTGAC ATTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTCGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCCCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACCCCCAAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAGT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAGTGTGGAT CGGAGCCGGG CCGCCGCGGG 
CTGGGCGTGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC GAAGGCGGAG AGCATCGCCA GTGCGCTGAG CATGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCACTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACACGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTA CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAAATCAGAG AAGGACACGG ATGCTGGATC ACTCTATAAG 
TCCAGCATGA TGCCCACCAA ACATGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTAGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAGGCCATCC GCCTGCCGCC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCACGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAAT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCGGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC CGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
CGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTCG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCACGGGGAG CGGGAGGGGC GCCAACAAGC GCATCACCTA CACCAACCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGCGAG ACCTATACCA TCGGCCTGCG GTTTGCGTCC 
CGTCAGAGAG CTGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_006069603.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006069665.2 Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X8, mRNA.

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CloneID	OBu66239
Clone ID Related Accession (Same CDS sequence)	XM_006069602.2 , XM_006069603.2 , XM_006069604.2
Accession Version	XM_006069603.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	nephrocystin-4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006069603.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCAGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACAGCG TTTCAGTGTG TCCTGAAGTG GCTCGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATTGGAGG TGGACTGCCA CCTGCGCGTG 
TCCCTCTTCG ATGTCACCTA TCGGCACTTC TTTGGGAGGA CGTGGAAGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGG 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCACGGG 
AGCCCGAGAG CCCTCCTCCA CCCACTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT TCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACCTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGTCTC 
CTTCCAGCTC ACGGAGAGTC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAACTTCTGG ATCTTGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC TCTGGATGTC 
GGCGCCTTGG AGATCCTGGA GAGGCGCCTG TGCGTGGGCG TGCACAATGG TTTGGGGTTT 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCCTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCATC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCGG ACGGCAATGC AGCTTCACTC 
ACCTCCTTGT CCAGCCTGGC ATGCATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
TTGGAAGCCA GCTCCGGACA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATGA GCTCTGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGACACA 
CCCACAAGGA ATGCAAGCGG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGCG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTCCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCGCT TTCCCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCACGC 
CGCCGCTCGG CCAAACCCTC ATCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC GCCTGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTATC ACGGGCGTCC 
CTGGCGCTGC TGCAGTCTGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GCTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACAC CACGACTGCG GCTCGTGGAG CTGGCCGATG CTGGGAAAAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT TGTAGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATTGACG TCTGGGATGG GGACTCCCTG 
CTTCTCGTCG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCAGCC 
GTGCAGGTCT CGCACGAGCT GGAGGTTGTG GCGACTGAAT ATGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACAGTGGTG 
AAGGGCCAGC TGCACTTGAC ATTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTCGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCCCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACCCCCAAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAGT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAGTGTGGAT CGGAGCCGGG CCGCCGCGGG 
CTGGGCGTGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC GAAGGCGGAG AGCATCGCCA GTGCGCTGAG CATGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCACTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACACGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTA CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAAATCAGAG AAGGACACGG ATGCTGGATC ACTCTATAAG 
TCCAGCATGA TGCCCACCAA ACATGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTAGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAGGCCATCC GCCTGCCGCC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCACGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAAT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCGGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC CGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
CGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTCG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCACGGGGAG CGGGAGGGGC GCCAACAAGC GCATCACCTA CACCAACCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGCGAG ACCTATACCA TCGGCCTGCG GTTTGCGTCC 
CGTCAGAGAG CTGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006069665.2
CDS	225..4520
Translation	MHDWHRIFDQNQLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLLEVDCHLRVSLFDVTYRHFFGRTWKSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLDLVISDCFREAGSPLDVGALEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVHHPAFAIVFQLEYVFNSPSGADGNAASLTSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASMSSEEVKQVESGTVQFQYSLSPEEHPDTPTRNASGPKAERRSFRKPPASPSSPPASPSQGLTAPQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLRLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLVGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTPPRPGARGPQGISQEGDAVRRRKLERMRAVRLRECGSEPGRRGLGVLAQQSLHAQHARDLQVIAAYRERTKAESIASALSMAITTQHTIHATLGTAEFFEFALRNPHNAQHTVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLYLRPRETAHIPFKFQTFSVGPWASAQASAELKSEKDTDAGSLYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQNVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPRRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPTGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFASRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_006069603.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006069603.2

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCAGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACAGCG TTTCAGTGTG TCCTGAAGTG GCTCGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATTGGAGG TGGACTGCCA CCTGCGCGTG 
TCCCTCTTCG ATGTCACCTA TCGGCACTTC TTTGGGAGGA CGTGGAAGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGG 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCACGGG 
AGCCCGAGAG CCCTCCTCCA CCCACTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT TCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACCTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGTCTC 
CTTCCAGCTC ACGGAGAGTC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAACTTCTGG ATCTTGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC TCTGGATGTC 
GGCGCCTTGG AGATCCTGGA GAGGCGCCTG TGCGTGGGCG TGCACAATGG TTTGGGGTTT 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCCTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCATC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCGG ACGGCAATGC AGCTTCACTC 
ACCTCCTTGT CCAGCCTGGC ATGCATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
TTGGAAGCCA GCTCCGGACA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATGA GCTCTGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGACACA 
CCCACAAGGA ATGCAAGCGG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGCG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTCCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCGCT TTCCCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCACGC 
CGCCGCTCGG CCAAACCCTC ATCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC GCCTGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTATC ACGGGCGTCC 
CTGGCGCTGC TGCAGTCTGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GCTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACAC CACGACTGCG GCTCGTGGAG CTGGCCGATG CTGGGAAAAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT TGTAGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATTGACG TCTGGGATGG GGACTCCCTG 
CTTCTCGTCG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCAGCC 
GTGCAGGTCT CGCACGAGCT GGAGGTTGTG GCGACTGAAT ATGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACAGTGGTG 
AAGGGCCAGC TGCACTTGAC ATTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTCGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCCCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACCCCCAAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAGT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAGTGTGGAT CGGAGCCGGG CCGCCGCGGG 
CTGGGCGTGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC GAAGGCGGAG AGCATCGCCA GTGCGCTGAG CATGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCACTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACACGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTA CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAAATCAGAG AAGGACACGG ATGCTGGATC ACTCTATAAG 
TCCAGCATGA TGCCCACCAA ACATGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTAGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAGGCCATCC GCCTGCCGCC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCACGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAAT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCGGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC CGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
CGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTCG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCACGGGGAG CGGGAGGGGC GCCAACAAGC GCATCACCTA CACCAACCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGCGAG ACCTATACCA TCGGCCTGCG GTTTGCGTCC 
CGTCAGAGAG CTGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_006069604.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006069666.2 Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBu66239
Clone ID Related Accession (Same CDS sequence)	XM_006069602.2 , XM_006069603.2 , XM_006069604.2
Accession Version	XM_006069604.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	nephrocystin-4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006069604.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCAGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACAGCG TTTCAGTGTG TCCTGAAGTG GCTCGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATTGGAGG TGGACTGCCA CCTGCGCGTG 
TCCCTCTTCG ATGTCACCTA TCGGCACTTC TTTGGGAGGA CGTGGAAGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGG 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCACGGG 
AGCCCGAGAG CCCTCCTCCA CCCACTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT TCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACCTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGTCTC 
CTTCCAGCTC ACGGAGAGTC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAACTTCTGG ATCTTGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC TCTGGATGTC 
GGCGCCTTGG AGATCCTGGA GAGGCGCCTG TGCGTGGGCG TGCACAATGG TTTGGGGTTT 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCCTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCATC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCGG ACGGCAATGC AGCTTCACTC 
ACCTCCTTGT CCAGCCTGGC ATGCATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
TTGGAAGCCA GCTCCGGACA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATGA GCTCTGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGACACA 
CCCACAAGGA ATGCAAGCGG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGCG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTCCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCGCT TTCCCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCACGC 
CGCCGCTCGG CCAAACCCTC ATCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC GCCTGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTATC ACGGGCGTCC 
CTGGCGCTGC TGCAGTCTGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GCTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACAC CACGACTGCG GCTCGTGGAG CTGGCCGATG CTGGGAAAAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT TGTAGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATTGACG TCTGGGATGG GGACTCCCTG 
CTTCTCGTCG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCAGCC 
GTGCAGGTCT CGCACGAGCT GGAGGTTGTG GCGACTGAAT ATGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACAGTGGTG 
AAGGGCCAGC TGCACTTGAC ATTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTCGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCCCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACCCCCAAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAGT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAGTGTGGAT CGGAGCCGGG CCGCCGCGGG 
CTGGGCGTGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC GAAGGCGGAG AGCATCGCCA GTGCGCTGAG CATGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCACTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACACGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTA CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAAATCAGAG AAGGACACGG ATGCTGGATC ACTCTATAAG 
TCCAGCATGA TGCCCACCAA ACATGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTAGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAGGCCATCC GCCTGCCGCC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCACGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAAT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCGGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC CGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
CGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTCG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCACGGGGAG CGGGAGGGGC GCCAACAAGC GCATCACCTA CACCAACCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGCGAG ACCTATACCA TCGGCCTGCG GTTTGCGTCC 
CGTCAGAGAG CTGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006069666.2
CDS	687..4982
Translation	MHDWHRIFDQNQLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLLEVDCHLRVSLFDVTYRHFFGRTWKSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLDLVISDCFREAGSPLDVGALEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVHHPAFAIVFQLEYVFNSPSGADGNAASLTSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASMSSEEVKQVESGTVQFQYSLSPEEHPDTPTRNASGPKAERRSFRKPPASPSSPPASPSQGLTAPQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLRLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLVGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTPPRPGARGPQGISQEGDAVRRRKLERMRAVRLRECGSEPGRRGLGVLAQQSLHAQHARDLQVIAAYRERTKAESIASALSMAITTQHTIHATLGTAEFFEFALRNPHNAQHTVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLYLRPRETAHIPFKFQTFSVGPWASAQASAELKSEKDTDAGSLYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQNVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPRRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPTGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFASRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_006069604.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006069604.2

ORF Insert Sequence:

ATGCACGACT GGCACAGGAT CTTTGACCAA AACCAGCTGG TCCCTCCGCA CCCACAGAGA 
GCACGCCAGC CTCTGAAGGA ATCGACAGCG TTTCAGTGTG TCCTGAAGTG GCTCGATGGC 
CCGCTAATTA AGCAGGGGGT GCTGGAGCTG CTATTGGAGG TGGACTGCCA CCTGCGCGTG 
TCCCTCTTCG ATGTCACCTA TCGGCACTTC TTTGGGAGGA CGTGGAAGAG CTCGCCGAGG 
CCTGCAGAGC CGCTCACCAG GCAGCCGGCC AGGATCGTCT TCAATGAGCC CTTGTATTTT 
CACACGTCCT TGAACCACCC TCATATTGTG GCCGTGGTGG AGGTGGTCGT TGAAGGCAGG 
AGACGAGACG GGACCCTCCA GACTCTGTCC TGTGGGTTTG GAATTCTTCG GATCTTCAGC 
CACAAGCCGG AATCTCCAAC TTCCACTTCC CAGGACAAAC GGCTGAGACT GTATCACGGG 
AGCCCGAGAG CCCTCCTCCA CCCACTCCTT CAGGACCCCA TAGAACAGAA CAAGCACATG 
ACCCTCATTG AGAACTGCAG CCTGCAGTAC ACGCTGAGGC CACACCCGCT TCTGGAGCCC 
GTGTTCCACC TTCTTCCCGA GAACCTGCTG GTGTCCGGTC TGCAGCAGAT ACCTGGTCTC 
CTTCCAGCTC ACGGAGAGTC AGGAGATGCC CTCCGGAAGC CCCGCCTGCA GAAGCCTCTG 
ACGTGCTACC TGGATGATCT GTTCTTCACG CTGTATCCCT CGCTGGAGAA GTTCGAGGAG 
GAACTTCTGG ATCTTGTGAT CAGCGATTGC TTCCGGGAGG CGGGCAGCCC TCTGGATGTC 
GGCGCCTTGG AGATCCTGGA GAGGCGCCTG TGCGTGGGCG TGCACAATGG TTTGGGGTTT 
GTGCAGCGGC CGCAGGTCCT CGTGCTGGTG CCCGAGCTGG AGGTGGCCTT GACACGCTCT 
GCCAGCTTCA GCCGGAAGGT CGGCTCCTCT TCCAAGGCCA GCTCTGGAAA CCAGGCGCTG 
GTCCTGAGAA GCCGTCTCCG ACTCCCTGAG ATGGTCCATC ACCCCGCGTT CGCCATTGTC 
TTCCAGCTGG AGTACGTGTT TAACAGCCCC TCGGGGGCGG ACGGCAATGC AGCTTCACTC 
ACCTCCTTGT CCAGCCTGGC ATGCATGCAC ATGGTTCGCT GGGCTGTCTG GAACCCCTCG 
TTGGAAGCCA GCTCCGGACA GGTGATGTTA CCTCTCCAGG GTGGCATCCA GTCCAACCCT 
TCGCACTGTC TGGTCTATAA GGTACCCCCA GCCAGCATGA GCTCTGAAGA GGTGAAGCAG 
GTGGAGTCAG GGACGGTCCA GTTCCAGTAC TCGCTGAGCC CTGAAGAACA CCCAGACACA 
CCCACAAGGA ATGCAAGCGG CCCCAAGGCA GAGCGGCGGT CTTTTAGGAA GCCACCCGCG 
TCCCCTTCAA GTCCACCAGC ATCGCCGTCC CAGGGGCTCA CCGCTCCCCA GGACTCACCC 
ATGGGACCAG GGCTGTCGCT TTCCCAGCTG GCGGCTTCCC CACCATCCCC AGCTCCACGC 
CGCCGCTCGG CCAAACCCTC ATCACAGCCA CCTGGGAGCC CTGTGCCCTC CCTGGTCCCA 
GGGGAGTTCC CCTTGGAGGG CAGGATCTCT CACCTGGAGG CTGACCTGAG CCAGACAGCC 
TTAGCCCTGG GAACGTCCGC TGCTGACCAC TTGCAGGAAC TGCCTTTCAC GCCTGTGCAC 
GCCCCGATCA TTGCAGGAAC CCAGACCAGG AGCTCTGGAG GGAAGCTATC ACGGGCGTCC 
CTGGCGCTGC TGCAGTCTGC TGGCTTTCCC GAGATTCTGG ACGCCAACAA GCAACCAGCT 
GAGGCTGTGA GTCCTACAGA CCCCGTGAAG TTCAGCCCTC AGAAGGAAGA ATCAGACTGT 
CTACAAGGCA ATGAGATAGT GCTGCAATTT CTTGCCTTTA GCAGGGTGCC CCAGGATGAG 
CAGGCAGCGC CATGGCCACA GACTGTGTAC TTCACCTTCC AATTCTACCG CTTCCCACCC 
GAGACCACAC CACGACTGCG GCTCGTGGAG CTGGCCGATG CTGGGAAAAC CAGCTCTGCC 
TCCCTGTCGC ACATCCTCGC CCAGATCCAG AAGGATGGCT CCTTTGATGC GGGGTCTCCG 
GGCTTTCAGC GGAAGTACCT TGTAGACCCC GGGTTCCTGA GACCCGGGGA GCAGCGCTGG 
TTCGCCCGCT ACCTGGCCAT GCAGACCCTG CAGATTGACG TCTGGGATGG GGACTCCCTG 
CTTCTCGTCG GATCTGCTGC CGTTCAGCTG AAGCACCTTC TCCGCCAGGG GCGGCCAGCC 
GTGCAGGTCT CGCACGAGCT GGAGGTTGTG GCGACTGAAT ATGAGCAGGA CGCCATGGTG 
GTGAGTGGAG ACATGACCAA GTTTGGCTGC GTCAGACCCA TCGACGTCCA CACAGTGGTG 
AAGGGCCAGC TGCACTTGAC ATTGGCCAAC GTGGGTCACT TGTGTGAACA GAGAGTAAGG 
AGTCCCAGTT CCCTGCCACC TTCCAGGTCG AGGGTCATCT CAAATGATGG AGTCGGCAAC 
TTCATGGGAG GCAGCCTCCT GACACCCGGG GGCCCCAGAT CAGTGAAAAA TGTGGTCCAA 
GCCCAGAAGC TGGCTGATGT GGACAGCGAA CTGGCCGCCC TGCTCTTCAC ACCCCCAAGA 
CCAGGGGCCC GGGGGCCCCA GGGCATCAGT CAAGAGGGGG ATGCCGTCCG CAGGCGCAAG 
CTGGAGCGCA TGAGGGCCGT GCGGCTGCGG GAGTGTGGAT CGGAGCCGGG CCGCCGCGGG 
CTGGGCGTGC TGGCCCAGCA GAGCCTCCAT GCCCAGCACG CCCGAGACCT GCAGGTCATT 
GCCGCCTACC GTGAGCGCAC GAAGGCGGAG AGCATCGCCA GTGCGCTGAG CATGGCCATC 
ACCACGCAGC ACACCATCCA CGCCACCCTA GGCACTGCCG AGTTCTTCGA GTTCGCCCTG 
AGGAACCCCC ACAACGCGCA GCACACGGTG ACCATCGAGA TAGACAACCC CGAGCTCAGT 
CTCATCCTGG ATGGTCAGGA GTGGCGGTAC TTCAAGGACA TGGCTGACTT GCACACACCC 
CTGGAGGAGG ACATGTTCCA CCTGCGGGGC AGCCTGGCCC CCCAACTCTA CCTGCGACCT 
CGGGAGACTG CCCACATCCC CTTCAAGTTC CAGACTTTCT CTGTGGGCCC CTGGGCCTCG 
GCACAGGCCT CTGCTGAGCT GAAATCAGAG AAGGACACGG ATGCTGGATC ACTCTATAAG 
TCCAGCATGA TGCCCACCAA ACATGCCAAG GTCCTGTTCC GCGCGGGCAG CGGCAAGCCC 
CTGGCCGTGC TCTGCCTGAC GGTGGAGCTG CAGCCTCACG TGGTAGACCA GGTCTTCCGC 
TTCCACCACC CGGAGCTCAC CTTCCTGAAG AAGGCCATCC GCCTGCCGCC CTGGCACACG 
CTTCCAGGTG CCCCTGTAGG GACTTCCAGT GAGGACCCCC CTTTCCACGT TCGATGTAGT 
GACCCGAACA TCATTTGTGA GACACAGAAT GTGGGCCCCG GGGAGCCCCG TGACGTGTTC 
CTGAAGGTGG CCAGTGGCCC TAGCCCGGAG GTCAAGGACT TCTTTGTCAC CATCTACGTA 
GACCGCTGGT TAGCAACACC CGTCCAGACC TGGCAGGTCC ACCTGCACTC GCTGCAGCGA 
GTGGACGTCT CCTGTATCAC TGGCCAGAGG ACCCGCCTGG CTCTCGTCCT CCGGGGGACG 
CAGACAGTTA GGAGAGTGCA GGCTTTCAGC TCACACCCCC AGGAGCTGAC GACGGACCCC 
AGAGGTGTGT TCGTGCTGCC TCCTCACGGG GTGCAGGACT TGCATGTGAG CGTCAGGCCC 
CGCAGGCCGG GCAGCCGCTT CGCGCACCTC CACCTGGTCG ACGTGGACGC CCACCAGCTG 
GTGGCCTCGT GGCTCCTGTG TCTCTCCTGC CGGCCGCCTC TCATCTCCAA GGCCTTCGAG 
ATAAAGCTGC CCACGGGGAG CGGGAGGGGC GCCAACAAGC GCATCACCTA CACCAACCCC 
TACCCCTCGC GGAGGGCCTA CCGCCTGCAC AGCGACCACC CCCACCTGCT GCAGTTCAAG 
GAGGACGCCT TCCAGGTCGG GGGAGGCGAG ACCTATACCA TCGGCCTGCG GTTTGCGTCC 
CGTCAGAGAG CTGGGGAGGA AGAGATCCTG ATTTACATCA ACGACCACGA AGACAAAAAC 
GAGGAGACCT TTTGCGTGAA GGTCATCTAC CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_025285821.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141606.1 Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBu66240
Clone ID Related Accession (Same CDS sequence)	XM_025285821.1
Accession Version	XM_025285821.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	nephrocystin-4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGC CGTCGCCGCC AGTTCCAAGC CGGGTCTCCA TCATAGCTCC CTGCACCCTC 
CTCTTCTCTG TGATTTTTAT AGGTCACAAC CACCTCTTTT GTGTGCTAAC TTGTATCCTG 
TGTTTCTGCC TCCCTTCAGA TACATTCCTC GAGGTCAACG TGGTTGTCTG CAGTGTTCTC 
CAGGGCCTGG CACAGGGTAA TCCCAGAGGC CCCAGGGACC TGGGGACTGG CAGAATGCAC 
GACTGGCACA GGATCTTTGA CCAAAACCAG CTGGTCCCTC CGCACCCACA GAGAGCACGC 
CAGCCTCTGA AGGAATCGAC AGCGTTTCAG TGTGTCCTGA AGTGGCTCGA TGGCCCGCTA 
ATTAAGCAGG GGGTGCTGGA GCTGCTATTG GAGGTGGACT GCCACCTGCG CGTGTCCCTC 
TTCGATGTCA CCTATCGGCA CTTCTTTGGG AGGACGTGGA AGAGCTCGCC GAGGCCTGCA 
GAGCCGCTCA CCAGGCAGCC GGCCAGGATC GTCTTCAATG AGCCCTTGTA TTTTCACACG 
TCCTTGAACC ACCCTCATAT TGTGGCCGTG GTGGAGGTGG TCGTTGAAGG CAGGAGACGA 
GACGGGACCC TCCAGACTCT GTCCTGTGGG TTTGGAATTC TTCGGATCTT CAGCCACAAG 
CCGGAATCTC CAACTTCCAC TTCCCAGGAC AAACGGCTGA GACTGTATCA CGGGAGCCCG 
AGAGCCCTCC TCCACCCACT CCTTCAGGAC CCCATAGAAC AGAACAAGCA CATGACCCTC 
ATTGAGAACT GCAGCCTGCA GTACACGCTG AGGCCACACC CGCTTCTGGA GCCCGTGTTC 
CACCTTCTTC CCGAGAACCT GCTGGTGTCC GGTCTGCAGC AGATACCTGG TCTCCTTCCA 
GCTCACGGAG AGTCAGGAGA TGCCCTCCGG AAGCCCCGCC TGCAGAAGCC TCTGACGTGC 
TACCTGGATG ATCTGTTCTT CACGCTGTAT CCCTCGCTGG AGAAGTTCGA GGAGGAACTT 
CTGGATCTTG TGATCAGCGA TTGCTTCCGG GAGGCGGGCA GCCCTCTGGA TGTCGGCGCC 
TTGGAGATCC TGGAGAGGCG CCTGTGCGTG GGCGTGCACA ATGGTTTGGG GTTTGTGCAG 
CGGCCGCAGG TCCTCGTGCT GGTGCCCGAG CTGGAGGTGG CCTTGACACG CTCTGCCAGC 
TTCAGCCGGA AGGTCGGCTC CTCTTCCAAG GCCAGCTCTG GAAACCAGGC GCTGGTCCTG 
AGAAGCCGTC TCCGACTCCC TGAGATGGTC CATCACCCCG CGTTCGCCAT TGTCTTCCAG 
CTGGAGTACG TGTTTAACAG CCCCTCGGGG GCGGACGGCA ATGCAGCTTC ACTCACCTCC 
TTGTCCAGCC TGGCATGCAT GCACATGGTT CGCTGGGCTG TCTGGAACCC CTCGTTGGAA 
GCCAGCTCCG GACAGGTGAT GTTACCTCTC CAGGGTGGCA TCCAGTCCAA CCCTTCGCAC 
TGTCTGGTCT ATAAGGTACC CCCAGCCAGC ATGAGCTCTG AAGAGGTGAA GCAGGTGGAG 
TCAGGGACGG TCCAGTTCCA GTACTCGCTG AGCCCTGAAG AACACCCAGA CACACCCACA 
AGGAATGCAA GCGGCCCCAA GGCAGAGCGG CGGTCTTTTA GGAAGCCACC CGCGTCCCCT 
TCAAGTCCAC CAGCATCGCC GTCCCAGGGG CTCACCGCTC CCCAGGACTC ACCCATGGGA 
CCAGGGCTGT CGCTTTCCCA GCTGGCGGCT TCCCCACCAT CCCCAGCTCC ACGCCGCCGC 
TCGGCCAAAC CCTCATCACA GCCACCTGGG AGCCCTGTGC CCTCCCTGGT CCCAGGGGAG 
TTCCCCTTGG AGGGCAGGAT CTCTCACCTG GAGGCTGACC TGAGCCAGAC AGCCTTAGCC 
CTGGGAACGT CCGCTGCTGA CCACTTGCAG GAACTGCCTT TCACGCCTGT GCACGCCCCG 
ATCATTGCAG GAACCCAGAC CAGGAGCTCT GGAGGGAAGC TATCACGGGC GTCCCTGGCG 
CTGCTGCAGT CTGCTGGCTT TCCCGAGATT CTGGACGCCA ACAAGCAACC AGCTGAGGCT 
GTGAGTCCTA CAGACCCCGT GAAGTTCAGC CCTCAGAAGG AAGAATCAGA CTGTCTACAA 
GGCAATGAGA TAGTGCTGCA ATTTCTTGCC TTTAGCAGGG TGCCCCAGGA TGAGCAGGCA 
GCGCCATGGC CACAGACTGT GTACTTCACC TTCCAATTCT ACCGCTTCCC ACCCGAGACC 
ACACCACGAC TGCGGCTCGT GGAGCTGGCC GATGCTGGGA AAACCAGCTC TGCCTCCCTG 
TCGCACATCC TCGCCCAGAT CCAGAAGGAT GGCTCCTTTG ATGCGGGGTC TCCGGGCTTT 
CAGCGGAAGT ACCTTGTAGA CCCCGGGTTC CTGAGACCCG GGGAGCAGCG CTGGTTCGCC 
CGCTACCTGG CCATGCAGAC CCTGCAGATT GACGTCTGGG ATGGGGACTC CCTGCTTCTC 
GTCGGATCTG CTGCCGTTCA GCTGAAGCAC CTTCTCCGCC AGGGGCGGCC AGCCGTGCAG 
GTCTCGCACG AGCTGGAGGT TGTGGCGACT GAATATGAGC AGGACGCCAT GGTGGTGAGT 
GGAGACATGA CCAAGTTTGG CTGCGTCAGA CCCATCGACG TCCACACAGT GGTGAAGGGC 
CAGCTGCACT TGACATTGGC CAACGTGGGT CACTTGTGTG AACAGAGAGT AAGGAGTCCC 
AGTTCCCTGC CACCTTCCAG GTCGAGGGTC ATCTCAAATG ATGGAGTCGG CAACTTCATG 
GGAGGCAGCC TCCTGACACC CGGGGGCCCC AGATCAGTGA AAAATGTGGT CCAAGCCCAG 
AAGCTGGCTG ATGTGGACAG CGAACTGGCC GCCCTGCTCT TCACACCCCC AAGACCAGGG 
GCCCGGGGGC CCCAGGGCAT CAGTCAAGAG GGGGATGCCG TCCGCAGGCG CAAGCTGGAG 
CGCATGAGGG CCGTGCGGCT GCGGGAGTGT GGATCGGAGC CGGGCCGCCG CGGGCTGGGC 
GTGCTGGCCC AGCAGAGCCT CCATGCCCAG CACGCCCGAG ACCTGCAGGT CATTGCCGCC 
TACCGTGAGC GCACGAAGGC GGAGAGCATC GCCAGTGCGC TGAGCATGGC CATCACCACG 
CAGCACACCA TCCACGCCAC CCTAGGCACT GCCGAGTTCT TCGAGTTCGC CCTGAGGAAC 
CCCCACAACG CGCAGCACAC GGTGACCATC GAGATAGACA ACCCCGAGCT CAGTCTCATC 
CTGGATGGTC AGGAGTGGCG GTACTTCAAG GACATGGCTG ACTTGCACAC ACCCCTGGAG 
GAGGACATGT TCCACCTGCG GGGCAGCCTG GCCCCCCAAC TCTACCTGCG ACCTCGGGAG 
ACTGCCCACA TCCCCTTCAA GTTCCAGACT TTCTCTGTGG GCCCCTGGGC CTCGGCACAG 
GCCTCTGCTG AGCTGAAATC AGAGAAGGAC ACGGATGCTG GATCACTCTA TAAGTCCAGC 
ATGATGCCCA CCAAACATGC CAAGGTCCTG TTCCGCGCGG GCAGCGGCAA GCCCCTGGCC 
GTGCTCTGCC TGACGGTGGA GCTGCAGCCT CACGTGGTAG ACCAGGTCTT CCGCTTCCAC 
CACCCGGAGC TCACCTTCCT GAAGAAGGCC ATCCGCCTGC CGCCCTGGCA CACGCTTCCA 
GGTGCCCCTG TAGGGACTTC CAGTGAGGAC CCCCCTTTCC ACGTTCGATG TAGTGACCCG 
AACATCATTT GTGAGACACA GAATGTGGGC CCCGGGGAGC CCCGTGACGT GTTCCTGAAG 
GTGGCCAGTG GCCCTAGCCC GGAGGTCAAG GACTTCTTTG TCACCATCTA CGTAGACCGC 
TGGTTAGCAA CACCCGTCCA GACCTGGCAG GTCCACCTGC ACTCGCTGCA GCGAGTGGAC 
GTCTCCTGTA TCACTGGCCA GAGGACCCGC CTGGCTCTCG TCCTCCGGGG GACGCAGACA 
GTTAGGAGAG TGCAGGCTTT CAGCTCACAC CCCCAGGAGC TGACGGAAGA ATTAACTGTG 
ACTCTGGAGT CAGTGTGGCC GCAATTGTAC ATCTCGGTGA TGGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141606.1
CDS	41..4168
Translation	MIVPSPPVPSRVSIIAPCTLLFSVIFIGHNHLFCVLTCILCFCLPSDTFLEVNVVVCSVLQGLAQGNPRGPRDLGTGRMHDWHRIFDQNQLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLLEVDCHLRVSLFDVTYRHFFGRTWKSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLDLVISDCFREAGSPLDVGALEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVHHPAFAIVFQLEYVFNSPSGADGNAASLTSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASMSSEEVKQVESGTVQFQYSLSPEEHPDTPTRNASGPKAERRSFRKPPASPSSPPASPSQGLTAPQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLRLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLVGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTPPRPGARGPQGISQEGDAVRRRKLERMRAVRLRECGSEPGRRGLGVLAQQSLHAQHARDLQVIAAYRERTKAESIASALSMAITTQHTIHATLGTAEFFEFALRNPHNAQHTVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLYLRPRETAHIPFKFQTFSVGPWASAQASAELKSEKDTDAGSLYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQNVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTEELTVTLESVWPQLYISVMG

Target ORF information:

RefSeq Version	XM_025285821.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025285821.1

ORF Insert Sequence:

ATGATTGTGC CGTCGCCGCC AGTTCCAAGC CGGGTCTCCA TCATAGCTCC CTGCACCCTC 
CTCTTCTCTG TGATTTTTAT AGGTCACAAC CACCTCTTTT GTGTGCTAAC TTGTATCCTG 
TGTTTCTGCC TCCCTTCAGA TACATTCCTC GAGGTCAACG TGGTTGTCTG CAGTGTTCTC 
CAGGGCCTGG CACAGGGTAA TCCCAGAGGC CCCAGGGACC TGGGGACTGG CAGAATGCAC 
GACTGGCACA GGATCTTTGA CCAAAACCAG CTGGTCCCTC CGCACCCACA GAGAGCACGC 
CAGCCTCTGA AGGAATCGAC AGCGTTTCAG TGTGTCCTGA AGTGGCTCGA TGGCCCGCTA 
ATTAAGCAGG GGGTGCTGGA GCTGCTATTG GAGGTGGACT GCCACCTGCG CGTGTCCCTC 
TTCGATGTCA CCTATCGGCA CTTCTTTGGG AGGACGTGGA AGAGCTCGCC GAGGCCTGCA 
GAGCCGCTCA CCAGGCAGCC GGCCAGGATC GTCTTCAATG AGCCCTTGTA TTTTCACACG 
TCCTTGAACC ACCCTCATAT TGTGGCCGTG GTGGAGGTGG TCGTTGAAGG CAGGAGACGA 
GACGGGACCC TCCAGACTCT GTCCTGTGGG TTTGGAATTC TTCGGATCTT CAGCCACAAG 
CCGGAATCTC CAACTTCCAC TTCCCAGGAC AAACGGCTGA GACTGTATCA CGGGAGCCCG 
AGAGCCCTCC TCCACCCACT CCTTCAGGAC CCCATAGAAC AGAACAAGCA CATGACCCTC 
ATTGAGAACT GCAGCCTGCA GTACACGCTG AGGCCACACC CGCTTCTGGA GCCCGTGTTC 
CACCTTCTTC CCGAGAACCT GCTGGTGTCC GGTCTGCAGC AGATACCTGG TCTCCTTCCA 
GCTCACGGAG AGTCAGGAGA TGCCCTCCGG AAGCCCCGCC TGCAGAAGCC TCTGACGTGC 
TACCTGGATG ATCTGTTCTT CACGCTGTAT CCCTCGCTGG AGAAGTTCGA GGAGGAACTT 
CTGGATCTTG TGATCAGCGA TTGCTTCCGG GAGGCGGGCA GCCCTCTGGA TGTCGGCGCC 
TTGGAGATCC TGGAGAGGCG CCTGTGCGTG GGCGTGCACA ATGGTTTGGG GTTTGTGCAG 
CGGCCGCAGG TCCTCGTGCT GGTGCCCGAG CTGGAGGTGG CCTTGACACG CTCTGCCAGC 
TTCAGCCGGA AGGTCGGCTC CTCTTCCAAG GCCAGCTCTG GAAACCAGGC GCTGGTCCTG 
AGAAGCCGTC TCCGACTCCC TGAGATGGTC CATCACCCCG CGTTCGCCAT TGTCTTCCAG 
CTGGAGTACG TGTTTAACAG CCCCTCGGGG GCGGACGGCA ATGCAGCTTC ACTCACCTCC 
TTGTCCAGCC TGGCATGCAT GCACATGGTT CGCTGGGCTG TCTGGAACCC CTCGTTGGAA 
GCCAGCTCCG GACAGGTGAT GTTACCTCTC CAGGGTGGCA TCCAGTCCAA CCCTTCGCAC 
TGTCTGGTCT ATAAGGTACC CCCAGCCAGC ATGAGCTCTG AAGAGGTGAA GCAGGTGGAG 
TCAGGGACGG TCCAGTTCCA GTACTCGCTG AGCCCTGAAG AACACCCAGA CACACCCACA 
AGGAATGCAA GCGGCCCCAA GGCAGAGCGG CGGTCTTTTA GGAAGCCACC CGCGTCCCCT 
TCAAGTCCAC CAGCATCGCC GTCCCAGGGG CTCACCGCTC CCCAGGACTC ACCCATGGGA 
CCAGGGCTGT CGCTTTCCCA GCTGGCGGCT TCCCCACCAT CCCCAGCTCC ACGCCGCCGC 
TCGGCCAAAC CCTCATCACA GCCACCTGGG AGCCCTGTGC CCTCCCTGGT CCCAGGGGAG 
TTCCCCTTGG AGGGCAGGAT CTCTCACCTG GAGGCTGACC TGAGCCAGAC AGCCTTAGCC 
CTGGGAACGT CCGCTGCTGA CCACTTGCAG GAACTGCCTT TCACGCCTGT GCACGCCCCG 
ATCATTGCAG GAACCCAGAC CAGGAGCTCT GGAGGGAAGC TATCACGGGC GTCCCTGGCG 
CTGCTGCAGT CTGCTGGCTT TCCCGAGATT CTGGACGCCA ACAAGCAACC AGCTGAGGCT 
GTGAGTCCTA CAGACCCCGT GAAGTTCAGC CCTCAGAAGG AAGAATCAGA CTGTCTACAA 
GGCAATGAGA TAGTGCTGCA ATTTCTTGCC TTTAGCAGGG TGCCCCAGGA TGAGCAGGCA 
GCGCCATGGC CACAGACTGT GTACTTCACC TTCCAATTCT ACCGCTTCCC ACCCGAGACC 
ACACCACGAC TGCGGCTCGT GGAGCTGGCC GATGCTGGGA AAACCAGCTC TGCCTCCCTG 
TCGCACATCC TCGCCCAGAT CCAGAAGGAT GGCTCCTTTG ATGCGGGGTC TCCGGGCTTT 
CAGCGGAAGT ACCTTGTAGA CCCCGGGTTC CTGAGACCCG GGGAGCAGCG CTGGTTCGCC 
CGCTACCTGG CCATGCAGAC CCTGCAGATT GACGTCTGGG ATGGGGACTC CCTGCTTCTC 
GTCGGATCTG CTGCCGTTCA GCTGAAGCAC CTTCTCCGCC AGGGGCGGCC AGCCGTGCAG 
GTCTCGCACG AGCTGGAGGT TGTGGCGACT GAATATGAGC AGGACGCCAT GGTGGTGAGT 
GGAGACATGA CCAAGTTTGG CTGCGTCAGA CCCATCGACG TCCACACAGT GGTGAAGGGC 
CAGCTGCACT TGACATTGGC CAACGTGGGT CACTTGTGTG AACAGAGAGT AAGGAGTCCC 
AGTTCCCTGC CACCTTCCAG GTCGAGGGTC ATCTCAAATG ATGGAGTCGG CAACTTCATG 
GGAGGCAGCC TCCTGACACC CGGGGGCCCC AGATCAGTGA AAAATGTGGT CCAAGCCCAG 
AAGCTGGCTG ATGTGGACAG CGAACTGGCC GCCCTGCTCT TCACACCCCC AAGACCAGGG 
GCCCGGGGGC CCCAGGGCAT CAGTCAAGAG GGGGATGCCG TCCGCAGGCG CAAGCTGGAG 
CGCATGAGGG CCGTGCGGCT GCGGGAGTGT GGATCGGAGC CGGGCCGCCG CGGGCTGGGC 
GTGCTGGCCC AGCAGAGCCT CCATGCCCAG CACGCCCGAG ACCTGCAGGT CATTGCCGCC 
TACCGTGAGC GCACGAAGGC GGAGAGCATC GCCAGTGCGC TGAGCATGGC CATCACCACG 
CAGCACACCA TCCACGCCAC CCTAGGCACT GCCGAGTTCT TCGAGTTCGC CCTGAGGAAC 
CCCCACAACG CGCAGCACAC GGTGACCATC GAGATAGACA ACCCCGAGCT CAGTCTCATC 
CTGGATGGTC AGGAGTGGCG GTACTTCAAG GACATGGCTG ACTTGCACAC ACCCCTGGAG 
GAGGACATGT TCCACCTGCG GGGCAGCCTG GCCCCCCAAC TCTACCTGCG ACCTCGGGAG 
ACTGCCCACA TCCCCTTCAA GTTCCAGACT TTCTCTGTGG GCCCCTGGGC CTCGGCACAG 
GCCTCTGCTG AGCTGAAATC AGAGAAGGAC ACGGATGCTG GATCACTCTA TAAGTCCAGC 
ATGATGCCCA CCAAACATGC CAAGGTCCTG TTCCGCGCGG GCAGCGGCAA GCCCCTGGCC 
GTGCTCTGCC TGACGGTGGA GCTGCAGCCT CACGTGGTAG ACCAGGTCTT CCGCTTCCAC 
CACCCGGAGC TCACCTTCCT GAAGAAGGCC ATCCGCCTGC CGCCCTGGCA CACGCTTCCA 
GGTGCCCCTG TAGGGACTTC CAGTGAGGAC CCCCCTTTCC ACGTTCGATG TAGTGACCCG 
AACATCATTT GTGAGACACA GAATGTGGGC CCCGGGGAGC CCCGTGACGT GTTCCTGAAG 
GTGGCCAGTG GCCCTAGCCC GGAGGTCAAG GACTTCTTTG TCACCATCTA CGTAGACCGC 
TGGTTAGCAA CACCCGTCCA GACCTGGCAG GTCCACCTGC ACTCGCTGCA GCGAGTGGAC 
GTCTCCTGTA TCACTGGCCA GAGGACCCGC CTGGCTCTCG TCCTCCGGGG GACGCAGACA 
GTTAGGAGAG TGCAGGCTTT CAGCTCACAC CCCCAGGAGC TGACGGAAGA ATTAACTGTG 
ACTCTGGAGT CAGTGTGGCC GCAATTGTAC ATCTCGGTGA TGGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_025285822.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141607.1 Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X10, mRNA.

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CloneID	OBu66241
Clone ID Related Accession (Same CDS sequence)	XM_025285822.1
Accession Version	XM_025285822.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	nephrocystin-4 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGC CGTCGCCGCC AGTTCCAAGC CGGGTCTCCA TCATAGCTCC CTGCACCCTC 
CTCTTCTCTG TGATTTTTAT AGGTCACAAC CACCTCTTTT GTGTGCTAAC TTGTATCCTG 
TGTTTCTGCC TCCCTTCAGA TACATTCCTC GAGGTCAACG TGGTTGTCTG CAGTGTTCTC 
CAGGGCCTGG CACAGGGTAA TCCCAGAGGC CCCAGGGACC TGGGGACTGG CAGAATGCAC 
GACTGGCACA GGATCTTTGA CCAAAACCAG CTGGTCCCTC CGCACCCACA GAGAGCACGC 
CAGCCTCTGA AGGAATCGAC AGCGTTTCAG TGTGTCCTGA AGTGGCTCGA TGGCCCGCTA 
ATTAAGCAGG GGGTGCTGGA GCTGCTATTG GAGGTGGACT GCCACCTGCG CGTGTCCCTC 
TTCGATGTCA CCTATCGGCA CTTCTTTGGG AGGACGTGGA AGAGCTCGCC GAGGCCTGCA 
GAGCCGCTCA CCAGGCAGCC GGCCAGGATC GTCTTCAATG AGCCCTTGTA TTTTCACACG 
TCCTTGAACC ACCCTCATAT TGTGGCCGTG GTGGAGGTGG TCGTTGAAGG CAGGAGACGA 
GACGGGACCC TCCAGACTCT GTCCTGTGGG TTTGGAATTC TTCGGATCTT CAGCCACAAG 
CCGGAATCTC CAACTTCCAC TTCCCAGGAC AAACGGCTGA GACTGTATCA CGGGAGCCCG 
AGAGCCCTCC TCCACCCACT CCTTCAGGAC CCCATAGAAC AGAACAAGCA CATGACCCTC 
ATTGAGAACT GCAGCCTGCA GTACACGCTG AGGCCACACC CGCTTCTGGA GCCCGTGTTC 
CACCTTCTTC CCGAGAACCT GCTGGTGTCC GGTCTGCAGC AGATACCTGG TCTCCTTCCA 
GCTCACGGAG AGTCAGGAGA TGCCCTCCGG AAGCCCCGCC TGCAGAAGCC TCTGACGTGC 
TACCTGGATG ATCTGTTCTT CACGCTGTAT CCCTCGCTGG AGAAGTTCGA GGAGGAACTT 
CTGGATCTTG TGATCAGCGA TTGCTTCCGG GAGGCGGGCA GCCCTCTGGA TGTCGGCGCC 
TTGGAGATCC TGGAGAGGCG CCTGTGCGTG GGCGTGCACA ATGGTTTGGG GTTTGTGCAG 
CGGCCGCAGG TCCTCGTGCT GGTGCCCGAG CTGGAGGTGG CCTTGACACG CTCTGCCAGC 
TTCAGCCGGA AGGTCGGCTC CTCTTCCAAG GCCAGCTCTG GAAACCAGGC GCTGGTCCTG 
AGAAGCCGTC TCCGACTCCC TGAGATGGTC CATCACCCCG CGTTCGCCAT TGTCTTCCAG 
CTGGAGTACG TGTTTAACAG CCCCTCGGGG GCGGACGGCA ATGCAGCTTC ACTCACCTCC 
TTGTCCAGCC TGGCATGCAT GCACATGGTT CGCTGGGCTG TCTGGAACCC CTCGTTGGAA 
GCCAGCTCCG GACAGGTGAT GTTACCTCTC CAGGGTGGCA TCCAGTCCAA CCCTTCGCAC 
TGTCTGGTCT ATAAGGTACC CCCAGCCAGC ATGAGCTCTG AAGAGGTGAA GCAGGTGGAG 
TCAGGGACGG TCCAGTTCCA GTACTCGCTG AGCCCTGAAG AACACCCAGA CACACCCACA 
AGGAATGCAA GCGGCCCCAA GGCAGAGCGG CGGTCTTTTA GGAAGCCACC CGCGTCCCCT 
TCAAGTCCAC CAGCATCGCC GTCCCAGGGG CTCACCGCTC CCCAGGACTC ACCCATGGGA 
CCAGGGCTGT CGCTTTCCCA GCTGGCGGCT TCCCCACCAT CCCCAGCTCC ACGCCGCCGC 
TCGGCCAAAC CCTCATCACA GCCACCTGGG AGCCCTGTGC CCTCCCTGGT CCCAGGGGAG 
TTCCCCTTGG AGGGCAGGAT CTCTCACCTG GAGGCTGACC TGAGCCAGAC AGCCTTAGCC 
CTGGGAACGT CCGCTGCTGA CCACTTGCAG GAACTGCCTT TCACGCCTGT GCACGCCCCG 
ATCATTGCAG GAACCCAGAC CAGGAGCTCT GGAGGGAAGC TATCACGGGC GTCCCTGGCG 
CTGCTGCAGT CTGCTGGCTT TCCCGAGATT CTGGACGCCA ACAAGCAACC AGCTGAGGCT 
GTGAGTCCTA CAGACCCCGT GAAGTTCAGC CCTCAGAAGG AAGAATCAGA CTGTCTACAA 
GGCAATGAGA TAGTGCTGCA ATTTCTTGCC TTTAGCAGGG TGCCCCAGGA TGAGCAGGCA 
GCGCCATGGC CACAGACTGT GTACTTCACC TTCCAATTCT ACCGCTTCCC ACCCGAGACC 
ACACCACGAC TGCGGCTCGT GGAGCTGGCC GATGCTGGGA AAACCAGCTC TGCCTCCCTG 
TCGCACATCC TCGCCCAGAT CCAGAAGGAT GGCTCCTTTG ATGCGGGGTC TCCGGGCTTT 
CAGCGGAAGT ACCTTGTAGA CCCCGGGTTC CTGAGACCCG GGGAGCAGCG CTGGTTCGCC 
CGCTACCTGG CCATGCAGAC CCTGCAGATT GACGTCTGGG ATGGGGACTC CCTGCTTCTC 
GTCGGATCTG CTGCCGTTCA GCTGAAGCAC CTTCTCCGCC AGGGGCGGCC AGCCGTGCAG 
GTCTCGCACG AGCTGGAGGT TGTGGCGACT GAATATGAGC AGGACGCCAT GGTGGTGAGT 
GGAGACATGA CCAAGTTTGG CTGCGTCAGA CCCATCGACG TCCACACAGT GGTGAAGGGC 
CAGCTGCACT TGACATTGGC CAACGTGGGT CACTTGTGTG AACAGAGAGT AAGGAGTCCC 
AGTTCCCTGC CACCTTCCAG GTCGAGGGTC ATCTCAAATG ATGGAGTCGG CAACTTCATG 
GGAGGCAGCC TCCTGACACC CGGGGGCCCC AGATCAGTGA AAAATGTGGT CCAAGCCCAG 
AAGCTGGCTG ATGTGGACAG CGAACTGGCC GCCCTGCTCT TCACACCCCC AAGACCAGGG 
GCCCGGGGGC CCCAGGGCAT CAGTCAAGAG GGGGATGCCG TCCGCAGGCG CAAGCTGGAG 
CGCATGAGGG CCGTGCGGCT GCGGGAGTGT GGATCGGAGC CGGGCCGCCG CGGGCTGGGC 
GTGCTGGCCC AGCAGAGCCT CCATGCCCAG CACGCCCGAG ACCTGCAGGT CATTGCCGCC 
TACCGTGAGC GCACGAAGGC GGAGAGCATC GCCAGTGCGC TGAGCATGGC CATCACCACG 
CAGCACACCA TCCACGCCAC CCTAGGCACT GCCGAGTTCT TCGAGTTCGC CCTGAGGAAC 
CCCCACAACG CGCAGCACAC GGTGACCATC GAGATAGACA ACCCCGAGCT CAGTCTCATC 
CTGGATGGTC AGGAGTGGCG GTACTTCAAG GACATGGCTG ACTTGCACAC ACCCCTGGAG 
GAGGACATGT TCCACCTGCG GGGCAGCCTG GCCCCCCAAC TCTACCTGCG ACCTCGGGAG 
ACTGCCCACA TCCCCTTCAA GTTCCAGACT TTCTCTGTGG GCCCCTGGGC CTCGGCACAG 
GCCTCTGCTG AGCTGAAATC AGAGAAGGAC ACGGATGCTG GATCACTCTA TAAGTCCAGC 
ATGATGCCCA CCAAACATGC CAAGGTCCTG TTCCGCGCGG GCAGCGGCAA GCCCCTGGCC 
GTGCTCTGCC TGACGGTGGA GCTGCAGCCT CACGTGGTAG ACCAGGTCTT CCGCTTCCAC 
CACCCGGAGC TCACCTTCCT GAAGAAGGCC ATCCGCCTGC CGCCCTGGCA CACGCTTCCA 
GGTGCCCCTG TAGGGACTTC CAGTGAGGAC CCCCCTTTCC ACGTTCGATG TAGTGACCCG 
AACATCATTT GTGAGACACA GAATGTGGGC CCCGGGGAGC CCCGTGACGT GTTCCTGAAG 
GTGGCCAGTG GCCCTAGCCC GGAGGTCAAG GACTTCTTTG TCACCATCTA CGTAGACCGC 
TGGTTAGCAA CACCCGTCCA GACCTGGCAG GTCCACCTGC ACTCGCTGCA GCGAGTGGAC 
GTCTCCTGTA TCACTGGCCA GAGGACCCGC CTGGCTCTCG TCCTCCGGGG GACGCAGACA 
GTTAGGAGAG TGCAGGCTTT CAGCTCACAC CCCCAGGAGC TGACGGTGGT CACCCCTGAA 
GAGTTGTGCT CCCTGGGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141607.1
CDS	41..4141
Translation	MIVPSPPVPSRVSIIAPCTLLFSVIFIGHNHLFCVLTCILCFCLPSDTFLEVNVVVCSVLQGLAQGNPRGPRDLGTGRMHDWHRIFDQNQLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLLEVDCHLRVSLFDVTYRHFFGRTWKSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLDLVISDCFREAGSPLDVGALEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVHHPAFAIVFQLEYVFNSPSGADGNAASLTSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASMSSEEVKQVESGTVQFQYSLSPEEHPDTPTRNASGPKAERRSFRKPPASPSSPPASPSQGLTAPQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLRLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLVGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTPPRPGARGPQGISQEGDAVRRRKLERMRAVRLRECGSEPGRRGLGVLAQQSLHAQHARDLQVIAAYRERTKAESIASALSMAITTQHTIHATLGTAEFFEFALRNPHNAQHTVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLYLRPRETAHIPFKFQTFSVGPWASAQASAELKSEKDTDAGSLYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQNVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTVVTPEELCSLG

Target ORF information:

RefSeq Version	XM_025285822.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025285822.1

ORF Insert Sequence:

ATGATTGTGC CGTCGCCGCC AGTTCCAAGC CGGGTCTCCA TCATAGCTCC CTGCACCCTC 
CTCTTCTCTG TGATTTTTAT AGGTCACAAC CACCTCTTTT GTGTGCTAAC TTGTATCCTG 
TGTTTCTGCC TCCCTTCAGA TACATTCCTC GAGGTCAACG TGGTTGTCTG CAGTGTTCTC 
CAGGGCCTGG CACAGGGTAA TCCCAGAGGC CCCAGGGACC TGGGGACTGG CAGAATGCAC 
GACTGGCACA GGATCTTTGA CCAAAACCAG CTGGTCCCTC CGCACCCACA GAGAGCACGC 
CAGCCTCTGA AGGAATCGAC AGCGTTTCAG TGTGTCCTGA AGTGGCTCGA TGGCCCGCTA 
ATTAAGCAGG GGGTGCTGGA GCTGCTATTG GAGGTGGACT GCCACCTGCG CGTGTCCCTC 
TTCGATGTCA CCTATCGGCA CTTCTTTGGG AGGACGTGGA AGAGCTCGCC GAGGCCTGCA 
GAGCCGCTCA CCAGGCAGCC GGCCAGGATC GTCTTCAATG AGCCCTTGTA TTTTCACACG 
TCCTTGAACC ACCCTCATAT TGTGGCCGTG GTGGAGGTGG TCGTTGAAGG CAGGAGACGA 
GACGGGACCC TCCAGACTCT GTCCTGTGGG TTTGGAATTC TTCGGATCTT CAGCCACAAG 
CCGGAATCTC CAACTTCCAC TTCCCAGGAC AAACGGCTGA GACTGTATCA CGGGAGCCCG 
AGAGCCCTCC TCCACCCACT CCTTCAGGAC CCCATAGAAC AGAACAAGCA CATGACCCTC 
ATTGAGAACT GCAGCCTGCA GTACACGCTG AGGCCACACC CGCTTCTGGA GCCCGTGTTC 
CACCTTCTTC CCGAGAACCT GCTGGTGTCC GGTCTGCAGC AGATACCTGG TCTCCTTCCA 
GCTCACGGAG AGTCAGGAGA TGCCCTCCGG AAGCCCCGCC TGCAGAAGCC TCTGACGTGC 
TACCTGGATG ATCTGTTCTT CACGCTGTAT CCCTCGCTGG AGAAGTTCGA GGAGGAACTT 
CTGGATCTTG TGATCAGCGA TTGCTTCCGG GAGGCGGGCA GCCCTCTGGA TGTCGGCGCC 
TTGGAGATCC TGGAGAGGCG CCTGTGCGTG GGCGTGCACA ATGGTTTGGG GTTTGTGCAG 
CGGCCGCAGG TCCTCGTGCT GGTGCCCGAG CTGGAGGTGG CCTTGACACG CTCTGCCAGC 
TTCAGCCGGA AGGTCGGCTC CTCTTCCAAG GCCAGCTCTG GAAACCAGGC GCTGGTCCTG 
AGAAGCCGTC TCCGACTCCC TGAGATGGTC CATCACCCCG CGTTCGCCAT TGTCTTCCAG 
CTGGAGTACG TGTTTAACAG CCCCTCGGGG GCGGACGGCA ATGCAGCTTC ACTCACCTCC 
TTGTCCAGCC TGGCATGCAT GCACATGGTT CGCTGGGCTG TCTGGAACCC CTCGTTGGAA 
GCCAGCTCCG GACAGGTGAT GTTACCTCTC CAGGGTGGCA TCCAGTCCAA CCCTTCGCAC 
TGTCTGGTCT ATAAGGTACC CCCAGCCAGC ATGAGCTCTG AAGAGGTGAA GCAGGTGGAG 
TCAGGGACGG TCCAGTTCCA GTACTCGCTG AGCCCTGAAG AACACCCAGA CACACCCACA 
AGGAATGCAA GCGGCCCCAA GGCAGAGCGG CGGTCTTTTA GGAAGCCACC CGCGTCCCCT 
TCAAGTCCAC CAGCATCGCC GTCCCAGGGG CTCACCGCTC CCCAGGACTC ACCCATGGGA 
CCAGGGCTGT CGCTTTCCCA GCTGGCGGCT TCCCCACCAT CCCCAGCTCC ACGCCGCCGC 
TCGGCCAAAC CCTCATCACA GCCACCTGGG AGCCCTGTGC CCTCCCTGGT CCCAGGGGAG 
TTCCCCTTGG AGGGCAGGAT CTCTCACCTG GAGGCTGACC TGAGCCAGAC AGCCTTAGCC 
CTGGGAACGT CCGCTGCTGA CCACTTGCAG GAACTGCCTT TCACGCCTGT GCACGCCCCG 
ATCATTGCAG GAACCCAGAC CAGGAGCTCT GGAGGGAAGC TATCACGGGC GTCCCTGGCG 
CTGCTGCAGT CTGCTGGCTT TCCCGAGATT CTGGACGCCA ACAAGCAACC AGCTGAGGCT 
GTGAGTCCTA CAGACCCCGT GAAGTTCAGC CCTCAGAAGG AAGAATCAGA CTGTCTACAA 
GGCAATGAGA TAGTGCTGCA ATTTCTTGCC TTTAGCAGGG TGCCCCAGGA TGAGCAGGCA 
GCGCCATGGC CACAGACTGT GTACTTCACC TTCCAATTCT ACCGCTTCCC ACCCGAGACC 
ACACCACGAC TGCGGCTCGT GGAGCTGGCC GATGCTGGGA AAACCAGCTC TGCCTCCCTG 
TCGCACATCC TCGCCCAGAT CCAGAAGGAT GGCTCCTTTG ATGCGGGGTC TCCGGGCTTT 
CAGCGGAAGT ACCTTGTAGA CCCCGGGTTC CTGAGACCCG GGGAGCAGCG CTGGTTCGCC 
CGCTACCTGG CCATGCAGAC CCTGCAGATT GACGTCTGGG ATGGGGACTC CCTGCTTCTC 
GTCGGATCTG CTGCCGTTCA GCTGAAGCAC CTTCTCCGCC AGGGGCGGCC AGCCGTGCAG 
GTCTCGCACG AGCTGGAGGT TGTGGCGACT GAATATGAGC AGGACGCCAT GGTGGTGAGT 
GGAGACATGA CCAAGTTTGG CTGCGTCAGA CCCATCGACG TCCACACAGT GGTGAAGGGC 
CAGCTGCACT TGACATTGGC CAACGTGGGT CACTTGTGTG AACAGAGAGT AAGGAGTCCC 
AGTTCCCTGC CACCTTCCAG GTCGAGGGTC ATCTCAAATG ATGGAGTCGG CAACTTCATG 
GGAGGCAGCC TCCTGACACC CGGGGGCCCC AGATCAGTGA AAAATGTGGT CCAAGCCCAG 
AAGCTGGCTG ATGTGGACAG CGAACTGGCC GCCCTGCTCT TCACACCCCC AAGACCAGGG 
GCCCGGGGGC CCCAGGGCAT CAGTCAAGAG GGGGATGCCG TCCGCAGGCG CAAGCTGGAG 
CGCATGAGGG CCGTGCGGCT GCGGGAGTGT GGATCGGAGC CGGGCCGCCG CGGGCTGGGC 
GTGCTGGCCC AGCAGAGCCT CCATGCCCAG CACGCCCGAG ACCTGCAGGT CATTGCCGCC 
TACCGTGAGC GCACGAAGGC GGAGAGCATC GCCAGTGCGC TGAGCATGGC CATCACCACG 
CAGCACACCA TCCACGCCAC CCTAGGCACT GCCGAGTTCT TCGAGTTCGC CCTGAGGAAC 
CCCCACAACG CGCAGCACAC GGTGACCATC GAGATAGACA ACCCCGAGCT CAGTCTCATC 
CTGGATGGTC AGGAGTGGCG GTACTTCAAG GACATGGCTG ACTTGCACAC ACCCCTGGAG 
GAGGACATGT TCCACCTGCG GGGCAGCCTG GCCCCCCAAC TCTACCTGCG ACCTCGGGAG 
ACTGCCCACA TCCCCTTCAA GTTCCAGACT TTCTCTGTGG GCCCCTGGGC CTCGGCACAG 
GCCTCTGCTG AGCTGAAATC AGAGAAGGAC ACGGATGCTG GATCACTCTA TAAGTCCAGC 
ATGATGCCCA CCAAACATGC CAAGGTCCTG TTCCGCGCGG GCAGCGGCAA GCCCCTGGCC 
GTGCTCTGCC TGACGGTGGA GCTGCAGCCT CACGTGGTAG ACCAGGTCTT CCGCTTCCAC 
CACCCGGAGC TCACCTTCCT GAAGAAGGCC ATCCGCCTGC CGCCCTGGCA CACGCTTCCA 
GGTGCCCCTG TAGGGACTTC CAGTGAGGAC CCCCCTTTCC ACGTTCGATG TAGTGACCCG 
AACATCATTT GTGAGACACA GAATGTGGGC CCCGGGGAGC CCCGTGACGT GTTCCTGAAG 
GTGGCCAGTG GCCCTAGCCC GGAGGTCAAG GACTTCTTTG TCACCATCTA CGTAGACCGC 
TGGTTAGCAA CACCCGTCCA GACCTGGCAG GTCCACCTGC ACTCGCTGCA GCGAGTGGAC 
GTCTCCTGTA TCACTGGCCA GAGGACCCGC CTGGCTCTCG TCCTCCGGGG GACGCAGACA 
GTTAGGAGAG TGCAGGCTTT CAGCTCACAC CCCCAGGAGC TGACGGTGGT CACCCCTGAA 
GAGTTGTGCT CCCTGGGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_025285815.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141600.1 Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X1, mRNA.

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CloneID	OBu66234
Clone ID Related Accession (Same CDS sequence)	XM_025285815.1
Accession Version	XM_025285815.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	nephrocystin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGC CGTCGCCGCC AGTTCCAAGC CGGGTCTCCA TCATAGCTCC CTGCACCCTC 
CTCTTCTCTG TGATTTTTAT AGGTCACAAC CACCTCTTTT GTGTGCTAAC TTGTATCCTG 
TGTTTCTGCC TCCCTTCAGA TACATTCCTC GAGGTCAACG TGGTTGTCTG CAGTGTTCTC 
CAGGGCCTGG CACAGGGTAA TCCCAGAGGC CCCAGGGACC TGGGGACTGG CAGAATGCAC 
GACTGGCACA GGATCTTTGA CCAAAACCAG CTGGTCCCTC CGCACCCACA GAGAGCACGC 
CAGCCTCTGA AGGAATCGAC AGCGTTTCAG TGTGTCCTGA AGTGGCTCGA TGGCCCGCTA 
ATTAAGCAGG GGGTGCTGGA GCTGCTATTG GAGGTGGACT GCCACCTGCG CGTGTCCCTC 
TTCGATGTCA CCTATCGGCA CTTCTTTGGG AGGACGTGGA AGAGCTCGCC GAGGCCTGCA 
GAGCCGCTCA CCAGGCAGCC GGCCAGGATC GTCTTCAATG AGCCCTTGTA TTTTCACACG 
TCCTTGAACC ACCCTCATAT TGTGGCCGTG GTGGAGGTGG TCGTTGAAGG CAGGAGACGA 
GACGGGACCC TCCAGACTCT GTCCTGTGGG TTTGGAATTC TTCGGATCTT CAGCCACAAG 
CCGGAATCTC CAACTTCCAC TTCCCAGGAC AAACGGCTGA GACTGTATCA CGGGAGCCCG 
AGAGCCCTCC TCCACCCACT CCTTCAGGAC CCCATAGAAC AGAACAAGCA CATGACCCTC 
ATTGAGAACT GCAGCCTGCA GTACACGCTG AGGCCACACC CGCTTCTGGA GCCCGTGTTC 
CACCTTCTTC CCGAGAACCT GCTGGTGTCC GGTCTGCAGC AGATACCTGG TCTCCTTCCA 
GCTCACGGAG AGTCAGGAGA TGCCCTCCGG AAGCCCCGCC TGCAGAAGCC TCTGACGTGC 
TACCTGGATG ATCTGTTCTT CACGCTGTAT CCCTCGCTGG AGAAGTTCGA GGAGGAACTT 
CTGGATCTTG TGATCAGCGA TTGCTTCCGG GAGGCGGGCA GCCCTCTGGA TGTCGGCGCC 
TTGGAGATCC TGGAGAGGCG CCTGTGCGTG GGCGTGCACA ATGGTTTGGG GTTTGTGCAG 
CGGCCGCAGG TCCTCGTGCT GGTGCCCGAG CTGGAGGTGG CCTTGACACG CTCTGCCAGC 
TTCAGCCGGA AGGTCGGCTC CTCTTCCAAG GCCAGCTCTG GAAACCAGGC GCTGGTCCTG 
AGAAGCCGTC TCCGACTCCC TGAGATGGTC CATCACCCCG CGTTCGCCAT TGTCTTCCAG 
CTGGAGTACG TGTTTAACAG CCCCTCGGGG GCGGACGGCA ATGCAGCTTC ACTCACCTCC 
TTGTCCAGCC TGGCATGCAT GCACATGGTT CGCTGGGCTG TCTGGAACCC CTCGTTGGAA 
GCCAGCTCCG GACAGGTGAT GTTACCTCTC CAGGGTGGCA TCCAGTCCAA CCCTTCGCAC 
TGTCTGGTCT ATAAGGTACC CCCAGCCAGC ATGAGCTCTG AAGAGGTGAA GCAGGTGGAG 
TCAGGGACGG TCCAGTTCCA GTACTCGCTG AGCCCTGAAG AACACCCAGA CACACCCACA 
AGGAATGCAA GCGGCCCCAA GGCAGAGCGG CGGTCTTTTA GGAAGCCACC CGCGTCCCCT 
TCAAGTCCAC CAGCATCGCC GTCCCAGGGG CTCACCGCTC CCCAGGACTC ACCCATGGGA 
CCAGGGCTGT CGCTTTCCCA GCTGGCGGCT TCCCCACCAT CCCCAGCTCC ACGCCGCCGC 
TCGGCCAAAC CCTCATCACA GCCACCTGGG AGCCCTGTGC CCTCCCTGGT CCCAGGGGAG 
TTCCCCTTGG AGGGCAGGAT CTCTCACCTG GAGGCTGACC TGAGCCAGAC AGCCTTAGCC 
CTGGGAACGT CCGCTGCTGA CCACTTGCAG GAACTGCCTT TCACGCCTGT GCACGCCCCG 
ATCATTGCAG GAACCCAGAC CAGGAGCTCT GGAGGGAAGC TATCACGGGC GTCCCTGGCG 
CTGCTGCAGT CTGCTGGCTT TCCCGAGATT CTGGACGCCA ACAAGCAACC AGCTGAGGCT 
GTGAGTCCTA CAGACCCCGT GAAGTTCAGC CCTCAGAAGG AAGAATCAGA CTGTCTACAA 
GGCAATGAGA TAGTGCTGCA ATTTCTTGCC TTTAGCAGGG TGCCCCAGGA TGAGCAGGCA 
GCGCCATGGC CACAGACTGT GTACTTCACC TTCCAATTCT ACCGCTTCCC ACCCGAGACC 
ACACCACGAC TGCGGCTCGT GGAGCTGGCC GATGCTGGGA AAACCAGCTC TGCCTCCCTG 
TCGCACATCC TCGCCCAGAT CCAGAAGGAT GGCTCCTTTG ATGCGGGGTC TCCGGGCTTT 
CAGCGGAAGT ACCTTGTAGA CCCCGGGTTC CTGAGACCCG GGGAGCAGCG CTGGTTCGCC 
CGCTACCTGG CCATGCAGAC CCTGCAGATT GACGTCTGGG ATGGGGACTC CCTGCTTCTC 
GTCGGATCTG CTGCCGTTCA GCTGAAGCAC CTTCTCCGCC AGGGGCGGCC AGCCGTGCAG 
GTCTCGCACG AGCTGGAGGT TGTGGCGACT GAATATGAGC AGGACGCCAT GGTGGTGAGT 
GGAGACATGA CCAAGTTTGG CTGCGTCAGA CCCATCGACG TCCACACAGT GGTGAAGGGC 
CAGCTGCACT TGACATTGGC CAACGTGGGT CACTTGTGTG AACAGAGAGT AAGGAGTCCC 
AGTTCCCTGC CACCTTCCAG GTCGAGGGTC ATCTCAAATG ATGGAGTCGG CAACTTCATG 
GGAGGCAGCC TCCTGACACC CGGGGGCCCC AGATCAGTGA AAAATGTGGT CCAAGCCCAG 
AAGCTGGCTG ATGTGGACAG CGAACTGGCC GCCCTGCTCT TCACACCCCC AAGACCAGGG 
GCCCGGGGGC CCCAGGGCAT CAGTCAAGAG GGGGATGCCG TCCGCAGGCG CAAGCTGGAG 
CGCATGAGGG CCGTGCGGCT GCGGGAGTGT GGATCGGAGC CGGGCCGCCG CGGGCTGGGC 
GTGCTGGCCC AGCAGAGCCT CCATGCCCAG CACGCCCGAG ACCTGCAGGT CATTGCCGCC 
TACCGTGAGC GCACGAAGGC GGAGAGCATC GCCAGTGCGC TGAGCATGGC CATCACCACG 
CAGCACACCA TCCACGCCAC CCTAGGCACT GCCGAGTTCT TCGAGTTCGC CCTGAGGAAC 
CCCCACAACG CGCAGCACAC GGTGACCATC GAGATAGACA ACCCCGAGCT CAGTCTCATC 
CTGGATGGTC AGGAGTGGCG GTACTTCAAG GACATGGCTG ACTTGCACAC ACCCCTGGAG 
GAGGACATGT TCCACCTGCG GGGCAGCCTG GCCCCCCAAC TCTACCTGCG ACCTCGGGAG 
ACTGCCCACA TCCCCTTCAA GTTCCAGACT TTCTCTGTGG GCCCCTGGGC CTCGGCACAG 
GCCTCTGCTG AGCTGAAATC AGAGAAGGAC ACGGATGCTG GATCACTCTA TAAGTCCAGC 
ATGATGCCCA CCAAACATGC CAAGGTCCTG TTCCGCGCGG GCAGCGGCAA GCCCCTGGCC 
GTGCTCTGCC TGACGGTGGA GCTGCAGCCT CACGTGGTAG ACCAGGTCTT CCGCTTCCAC 
CACCCGGAGC TCACCTTCCT GAAGAAGGCC ATCCGCCTGC CGCCCTGGCA CACGCTTCCA 
GGTGCCCCTG TAGGGACTTC CAGTGAGGAC CCCCCTTTCC ACGTTCGATG TAGTGACCCG 
AACATCATTT GTGAGACACA GAATGTGGGC CCCGGGGAGC CCCGTGACGT GTTCCTGAAG 
GTGGCCAGTG GCCCTAGCCC GGAGGTCAAG GACTTCTTTG TCACCATCTA CGTAGACCGC 
TGGTTAGCAA CACCCGTCCA GACCTGGCAG GTCCACCTGC ACTCGCTGCA GCGAGTGGAC 
GTCTCCTGTA TCACTGGCCA GAGGACCCGC CTGGCTCTCG TCCTCCGGGG GACGCAGACA 
GTTAGGAGAG TGCAGGCTTT CAGCTCACAC CCCCAGGAGC TGACGACGGA CCCCAGAGGT 
GTGTTCGTGC TGCCTCCTCA CGGGGTGCAG GACTTGCATG TGAGCGTCAG GCCCCGCAGG 
CCGGGCAGCC GCTTCGCGCA CCTCCACCTG GTCGACGTGG ACGCCCACCA GCTGGTGGCC 
TCGTGGCTCC TGTGTCTCTC CTGCCGGCCG CCTCTCATCT CCAAGGCCTT CGAGATAAAG 
CTGCCCACGG GGAGCGGGAG GGGCGCCAAC AAGCGCATCA CCTACACCAA CCCCTACCCC 
TCGCGGAGGG CCTACCGCCT GCACAGCGAC CACCCCCACC TGCTGCAGTT CAAGGAGGAC 
GCCTTCCAGG TCGGGGGAGG CGAGACCTAT ACCATCGGCC TGCGGTTTGC GTCCCGTCAG 
AGAGCTGGGG AGGAAGAGAT CCTGATTTAC ATCAACGACC ACGAAGACAA AAACGAGGAG 
ACCTTTTGCG TGAAGGTCAT CTACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141600.1
CDS	41..4570
Translation	MIVPSPPVPSRVSIIAPCTLLFSVIFIGHNHLFCVLTCILCFCLPSDTFLEVNVVVCSVLQGLAQGNPRGPRDLGTGRMHDWHRIFDQNQLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLLEVDCHLRVSLFDVTYRHFFGRTWKSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLDLVISDCFREAGSPLDVGALEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVHHPAFAIVFQLEYVFNSPSGADGNAASLTSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASMSSEEVKQVESGTVQFQYSLSPEEHPDTPTRNASGPKAERRSFRKPPASPSSPPASPSQGLTAPQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLRLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLVGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRSVKNVVQAQKLADVDSELAALLFTPPRPGARGPQGISQEGDAVRRRKLERMRAVRLRECGSEPGRRGLGVLAQQSLHAQHARDLQVIAAYRERTKAESIASALSMAITTQHTIHATLGTAEFFEFALRNPHNAQHTVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLYLRPRETAHIPFKFQTFSVGPWASAQASAELKSEKDTDAGSLYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQNVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPRRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPTGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFASRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_025285815.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025285815.1

ORF Insert Sequence:

ATGATTGTGC CGTCGCCGCC AGTTCCAAGC CGGGTCTCCA TCATAGCTCC CTGCACCCTC 
CTCTTCTCTG TGATTTTTAT AGGTCACAAC CACCTCTTTT GTGTGCTAAC TTGTATCCTG 
TGTTTCTGCC TCCCTTCAGA TACATTCCTC GAGGTCAACG TGGTTGTCTG CAGTGTTCTC 
CAGGGCCTGG CACAGGGTAA TCCCAGAGGC CCCAGGGACC TGGGGACTGG CAGAATGCAC 
GACTGGCACA GGATCTTTGA CCAAAACCAG CTGGTCCCTC CGCACCCACA GAGAGCACGC 
CAGCCTCTGA AGGAATCGAC AGCGTTTCAG TGTGTCCTGA AGTGGCTCGA TGGCCCGCTA 
ATTAAGCAGG GGGTGCTGGA GCTGCTATTG GAGGTGGACT GCCACCTGCG CGTGTCCCTC 
TTCGATGTCA CCTATCGGCA CTTCTTTGGG AGGACGTGGA AGAGCTCGCC GAGGCCTGCA 
GAGCCGCTCA CCAGGCAGCC GGCCAGGATC GTCTTCAATG AGCCCTTGTA TTTTCACACG 
TCCTTGAACC ACCCTCATAT TGTGGCCGTG GTGGAGGTGG TCGTTGAAGG CAGGAGACGA 
GACGGGACCC TCCAGACTCT GTCCTGTGGG TTTGGAATTC TTCGGATCTT CAGCCACAAG 
CCGGAATCTC CAACTTCCAC TTCCCAGGAC AAACGGCTGA GACTGTATCA CGGGAGCCCG 
AGAGCCCTCC TCCACCCACT CCTTCAGGAC CCCATAGAAC AGAACAAGCA CATGACCCTC 
ATTGAGAACT GCAGCCTGCA GTACACGCTG AGGCCACACC CGCTTCTGGA GCCCGTGTTC 
CACCTTCTTC CCGAGAACCT GCTGGTGTCC GGTCTGCAGC AGATACCTGG TCTCCTTCCA 
GCTCACGGAG AGTCAGGAGA TGCCCTCCGG AAGCCCCGCC TGCAGAAGCC TCTGACGTGC 
TACCTGGATG ATCTGTTCTT CACGCTGTAT CCCTCGCTGG AGAAGTTCGA GGAGGAACTT 
CTGGATCTTG TGATCAGCGA TTGCTTCCGG GAGGCGGGCA GCCCTCTGGA TGTCGGCGCC 
TTGGAGATCC TGGAGAGGCG CCTGTGCGTG GGCGTGCACA ATGGTTTGGG GTTTGTGCAG 
CGGCCGCAGG TCCTCGTGCT GGTGCCCGAG CTGGAGGTGG CCTTGACACG CTCTGCCAGC 
TTCAGCCGGA AGGTCGGCTC CTCTTCCAAG GCCAGCTCTG GAAACCAGGC GCTGGTCCTG 
AGAAGCCGTC TCCGACTCCC TGAGATGGTC CATCACCCCG CGTTCGCCAT TGTCTTCCAG 
CTGGAGTACG TGTTTAACAG CCCCTCGGGG GCGGACGGCA ATGCAGCTTC ACTCACCTCC 
TTGTCCAGCC TGGCATGCAT GCACATGGTT CGCTGGGCTG TCTGGAACCC CTCGTTGGAA 
GCCAGCTCCG GACAGGTGAT GTTACCTCTC CAGGGTGGCA TCCAGTCCAA CCCTTCGCAC 
TGTCTGGTCT ATAAGGTACC CCCAGCCAGC ATGAGCTCTG AAGAGGTGAA GCAGGTGGAG 
TCAGGGACGG TCCAGTTCCA GTACTCGCTG AGCCCTGAAG AACACCCAGA CACACCCACA 
AGGAATGCAA GCGGCCCCAA GGCAGAGCGG CGGTCTTTTA GGAAGCCACC CGCGTCCCCT 
TCAAGTCCAC CAGCATCGCC GTCCCAGGGG CTCACCGCTC CCCAGGACTC ACCCATGGGA 
CCAGGGCTGT CGCTTTCCCA GCTGGCGGCT TCCCCACCAT CCCCAGCTCC ACGCCGCCGC 
TCGGCCAAAC CCTCATCACA GCCACCTGGG AGCCCTGTGC CCTCCCTGGT CCCAGGGGAG 
TTCCCCTTGG AGGGCAGGAT CTCTCACCTG GAGGCTGACC TGAGCCAGAC AGCCTTAGCC 
CTGGGAACGT CCGCTGCTGA CCACTTGCAG GAACTGCCTT TCACGCCTGT GCACGCCCCG 
ATCATTGCAG GAACCCAGAC CAGGAGCTCT GGAGGGAAGC TATCACGGGC GTCCCTGGCG 
CTGCTGCAGT CTGCTGGCTT TCCCGAGATT CTGGACGCCA ACAAGCAACC AGCTGAGGCT 
GTGAGTCCTA CAGACCCCGT GAAGTTCAGC CCTCAGAAGG AAGAATCAGA CTGTCTACAA 
GGCAATGAGA TAGTGCTGCA ATTTCTTGCC TTTAGCAGGG TGCCCCAGGA TGAGCAGGCA 
GCGCCATGGC CACAGACTGT GTACTTCACC TTCCAATTCT ACCGCTTCCC ACCCGAGACC 
ACACCACGAC TGCGGCTCGT GGAGCTGGCC GATGCTGGGA AAACCAGCTC TGCCTCCCTG 
TCGCACATCC TCGCCCAGAT CCAGAAGGAT GGCTCCTTTG ATGCGGGGTC TCCGGGCTTT 
CAGCGGAAGT ACCTTGTAGA CCCCGGGTTC CTGAGACCCG GGGAGCAGCG CTGGTTCGCC 
CGCTACCTGG CCATGCAGAC CCTGCAGATT GACGTCTGGG ATGGGGACTC CCTGCTTCTC 
GTCGGATCTG CTGCCGTTCA GCTGAAGCAC CTTCTCCGCC AGGGGCGGCC AGCCGTGCAG 
GTCTCGCACG AGCTGGAGGT TGTGGCGACT GAATATGAGC AGGACGCCAT GGTGGTGAGT 
GGAGACATGA CCAAGTTTGG CTGCGTCAGA CCCATCGACG TCCACACAGT GGTGAAGGGC 
CAGCTGCACT TGACATTGGC CAACGTGGGT CACTTGTGTG AACAGAGAGT AAGGAGTCCC 
AGTTCCCTGC CACCTTCCAG GTCGAGGGTC ATCTCAAATG ATGGAGTCGG CAACTTCATG 
GGAGGCAGCC TCCTGACACC CGGGGGCCCC AGATCAGTGA AAAATGTGGT CCAAGCCCAG 
AAGCTGGCTG ATGTGGACAG CGAACTGGCC GCCCTGCTCT TCACACCCCC AAGACCAGGG 
GCCCGGGGGC CCCAGGGCAT CAGTCAAGAG GGGGATGCCG TCCGCAGGCG CAAGCTGGAG 
CGCATGAGGG CCGTGCGGCT GCGGGAGTGT GGATCGGAGC CGGGCCGCCG CGGGCTGGGC 
GTGCTGGCCC AGCAGAGCCT CCATGCCCAG CACGCCCGAG ACCTGCAGGT CATTGCCGCC 
TACCGTGAGC GCACGAAGGC GGAGAGCATC GCCAGTGCGC TGAGCATGGC CATCACCACG 
CAGCACACCA TCCACGCCAC CCTAGGCACT GCCGAGTTCT TCGAGTTCGC CCTGAGGAAC 
CCCCACAACG CGCAGCACAC GGTGACCATC GAGATAGACA ACCCCGAGCT CAGTCTCATC 
CTGGATGGTC AGGAGTGGCG GTACTTCAAG GACATGGCTG ACTTGCACAC ACCCCTGGAG 
GAGGACATGT TCCACCTGCG GGGCAGCCTG GCCCCCCAAC TCTACCTGCG ACCTCGGGAG 
ACTGCCCACA TCCCCTTCAA GTTCCAGACT TTCTCTGTGG GCCCCTGGGC CTCGGCACAG 
GCCTCTGCTG AGCTGAAATC AGAGAAGGAC ACGGATGCTG GATCACTCTA TAAGTCCAGC 
ATGATGCCCA CCAAACATGC CAAGGTCCTG TTCCGCGCGG GCAGCGGCAA GCCCCTGGCC 
GTGCTCTGCC TGACGGTGGA GCTGCAGCCT CACGTGGTAG ACCAGGTCTT CCGCTTCCAC 
CACCCGGAGC TCACCTTCCT GAAGAAGGCC ATCCGCCTGC CGCCCTGGCA CACGCTTCCA 
GGTGCCCCTG TAGGGACTTC CAGTGAGGAC CCCCCTTTCC ACGTTCGATG TAGTGACCCG 
AACATCATTT GTGAGACACA GAATGTGGGC CCCGGGGAGC CCCGTGACGT GTTCCTGAAG 
GTGGCCAGTG GCCCTAGCCC GGAGGTCAAG GACTTCTTTG TCACCATCTA CGTAGACCGC 
TGGTTAGCAA CACCCGTCCA GACCTGGCAG GTCCACCTGC ACTCGCTGCA GCGAGTGGAC 
GTCTCCTGTA TCACTGGCCA GAGGACCCGC CTGGCTCTCG TCCTCCGGGG GACGCAGACA 
GTTAGGAGAG TGCAGGCTTT CAGCTCACAC CCCCAGGAGC TGACGACGGA CCCCAGAGGT 
GTGTTCGTGC TGCCTCCTCA CGGGGTGCAG GACTTGCATG TGAGCGTCAG GCCCCGCAGG 
CCGGGCAGCC GCTTCGCGCA CCTCCACCTG GTCGACGTGG ACGCCCACCA GCTGGTGGCC 
TCGTGGCTCC TGTGTCTCTC CTGCCGGCCG CCTCTCATCT CCAAGGCCTT CGAGATAAAG 
CTGCCCACGG GGAGCGGGAG GGGCGCCAAC AAGCGCATCA CCTACACCAA CCCCTACCCC 
TCGCGGAGGG CCTACCGCCT GCACAGCGAC CACCCCCACC TGCTGCAGTT CAAGGAGGAC 
GCCTTCCAGG TCGGGGGAGG CGAGACCTAT ACCATCGGCC TGCGGTTTGC GTCCCGTCAG 
AGAGCTGGGG AGGAAGAGAT CCTGATTTAC ATCAACGACC ACGAAGACAA AAACGAGGAG 
ACCTTTTGCG TGAAGGTCAT CTACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_025285817.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141602.1 Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X2, mRNA.

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CloneID	OBu66235
Clone ID Related Accession (Same CDS sequence)	XM_025285817.1
Accession Version	XM_025285817.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	nephrocystin-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGC CGTCGCCGCC AGTTCCAAGC CGGGTCTCCA TCATAGCTCC CTGCACCCTC 
CTCTTCTCTG TGATTTTTAT AGGTCACAAC CACCTCTTTT GTGTGCTAAC TTGTATCCTG 
TGTTTCTGCC TCCCTTCAGA TACATTCCTC GAGGTCAACG TGGTTGTCTG CAGTGTTCTC 
CAGGGCCTGG CACAGGGTAA TCCCAGAGGC CCCAGGGACC TGGGGACTGG CAGAATGCAC 
GACTGGCACA GGATCTTTGA CCAAAACCAG CTGGTCCCTC CGCACCCACA GAGAGCACGC 
CAGCCTCTGA AGGAATCGAC AGCGTTTCAG TGTGTCCTGA AGTGGCTCGA TGGCCCGCTA 
ATTAAGCAGG GGGTGCTGGA GCTGCTATTG GAGGTGGACT GCCACCTGCG CGTGTCCCTC 
TTCGATGTCA CCTATCGGCA CTTCTTTGGG AGGACGTGGA AGAGCTCGCC GAGGCCTGCA 
GAGCCGCTCA CCAGGCAGCC GGCCAGGATC GTCTTCAATG AGCCCTTGTA TTTTCACACG 
TCCTTGAACC ACCCTCATAT TGTGGCCGTG GTGGAGGTGG TCGTTGAAGG CAGGAGACGA 
GACGGGACCC TCCAGACTCT GTCCTGTGGG TTTGGAATTC TTCGGATCTT CAGCCACAAG 
CCGGAATCTC CAACTTCCAC TTCCCAGGAC AAACGGCTGA GACTGTATCA CGGGAGCCCG 
AGAGCCCTCC TCCACCCACT CCTTCAGGAC CCCATAGAAC AGAACAAGCA CATGACCCTC 
ATTGAGAACT GCAGCCTGCA GTACACGCTG AGGCCACACC CGCTTCTGGA GCCCGTGTTC 
CACCTTCTTC CCGAGAACCT GCTGGTGTCC GGTCTGCAGC AGATACCTGG TCTCCTTCCA 
GCTCACGGAG AGTCAGGAGA TGCCCTCCGG AAGCCCCGCC TGCAGAAGCC TCTGACGTGC 
TACCTGGATG ATCTGTTCTT CACGCTGTAT CCCTCGCTGG AGAAGTTCGA GGAGGAACTT 
CTGGATCTTG TGATCAGCGA TTGCTTCCGG GAGGCGGGCA GCCCTCTGGA TGTCGGCGCC 
TTGGAGATCC TGGAGAGGCG CCTGTGCGTG GGCGTGCACA ATGGTTTGGG GTTTGTGCAG 
CGGCCGCAGG TCCTCGTGCT GGTGCCCGAG CTGGAGGTGG CCTTGACACG CTCTGCCAGC 
TTCAGCCGGA AGGTCGGCTC CTCTTCCAAG GCCAGCTCTG GAAACCAGGC GCTGGTCCTG 
AGAAGCCGTC TCCGACTCCC TGAGATGGTC CATCACCCCG CGTTCGCCAT TGTCTTCCAG 
CTGGAGTACG TGTTTAACAG CCCCTCGGGG GCGGACGGCA ATGCAGCTTC ACTCACCTCC 
TTGTCCAGCC TGGCATGCAT GCACATGGTT CGCTGGGCTG TCTGGAACCC CTCGTTGGAA 
GCCAGCTCCG GACAGGTGAT GTTACCTCTC CAGGGTGGCA TCCAGTCCAA CCCTTCGCAC 
TGTCTGGTCT ATAAGGTACC CCCAGCCAGC ATGAGCTCTG AAGAGGTGAA GCAGGTGGAG 
TCAGGGACGG TCCAGTTCCA GTACTCGCTG AGCCCTGAAG AACACCCAGA CACACCCACA 
AGGAATGCAA GCGGCCCCAA GGCAGAGCGG CGGTCTTTTA GGAAGCCACC CGCGTCCCCT 
TCAAGTCCAC CAGCATCGCC GTCCCAGGGG CTCACCGCTC CCCAGGACTC ACCCATGGGA 
CCAGGGCTGT CGCTTTCCCA GCTGGCGGCT TCCCCACCAT CCCCAGCTCC ACGCCGCCGC 
TCGGCCAAAC CCTCATCACA GCCACCTGGG AGCCCTGTGC CCTCCCTGGT CCCAGGGGAG 
TTCCCCTTGG AGGGCAGGAT CTCTCACCTG GAGGCTGACC TGAGCCAGAC AGCCTTAGCC 
CTGGGAACGT CCGCTGCTGA CCACTTGCAG GAACTGCCTT TCACGCCTGT GCACGCCCCG 
ATCATTGCAG GAACCCAGAC CAGGAGCTCT GGAGGGAAGC TATCACGGGC GTCCCTGGCG 
CTGCTGCAGT CTGCTGGCTT TCCCGAGATT CTGGACGCCA ACAAGCAACC AGCTGAGGCT 
GTGAGTCCTA CAGACCCCGT GAAGTTCAGC CCTCAGAAGG AAGAATCAGA CTGTCTACAA 
GGCAATGAGA TAGTGCTGCA ATTTCTTGCC TTTAGCAGGG TGCCCCAGGA TGAGCAGGCA 
GCGCCATGGC CACAGACTGT GTACTTCACC TTCCAATTCT ACCGCTTCCC ACCCGAGACC 
ACACCACGAC TGCGGCTCGT GGAGCTGGCC GATGCTGGGA AAACCAGCTC TGCCTCCCTG 
TCGCACATCC TCGCCCAGAT CCAGAAGGAT GGCTCCTTTG ATGCGGGGTC TCCGGGCTTT 
CAGCGGAAGT ACCTTGTAGA CCCCGGGTTC CTGAGACCCG GGGAGCAGCG CTGGTTCGCC 
CGCTACCTGG CCATGCAGAC CCTGCAGATT GACGTCTGGG ATGGGGACTC CCTGCTTCTC 
GTCGGATCTG CTGCCGTTCA GCTGAAGCAC CTTCTCCGCC AGGGGCGGCC AGCCGTGCAG 
GTCTCGCACG AGCTGGAGGT TGTGGCGACT GAATATGAGC AGGACGCCAT GGTGGTGAGT 
GGAGACATGA CCAAGTTTGG CTGCGTCAGA CCCATCGACG TCCACACAGT GGTGAAGGGC 
CAGCTGCACT TGACATTGGC CAACGTGGGT CACTTGTGTG AACAGAGAGT AAGGAGTCCC 
AGTTCCCTGC CACCTTCCAG GTCGAGGGTC ATCTCAAATG ATGGAGTCGG CAACTTCATG 
GGAGGCAGCC TCCTGACACC CGGGGGCCCC AGATTGAAAA ATGTGGTCCA AGCCCAGAAG 
CTGGCTGATG TGGACAGCGA ACTGGCCGCC CTGCTCTTCA CACCCCCAAG ACCAGGGGCC 
CGGGGGCCCC AGGGCATCAG TCAAGAGGGG GATGCCGTCC GCAGGCGCAA GCTGGAGCGC 
ATGAGGGCCG TGCGGCTGCG GGAGTGTGGA TCGGAGCCGG GCCGCCGCGG GCTGGGCGTG 
CTGGCCCAGC AGAGCCTCCA TGCCCAGCAC GCCCGAGACC TGCAGGTCAT TGCCGCCTAC 
CGTGAGCGCA CGAAGGCGGA GAGCATCGCC AGTGCGCTGA GCATGGCCAT CACCACGCAG 
CACACCATCC ACGCCACCCT AGGCACTGCC GAGTTCTTCG AGTTCGCCCT GAGGAACCCC 
CACAACGCGC AGCACACGGT GACCATCGAG ATAGACAACC CCGAGCTCAG TCTCATCCTG 
GATGGTCAGG AGTGGCGGTA CTTCAAGGAC ATGGCTGACT TGCACACACC CCTGGAGGAG 
GACATGTTCC ACCTGCGGGG CAGCCTGGCC CCCCAACTCT ACCTGCGACC TCGGGAGACT 
GCCCACATCC CCTTCAAGTT CCAGACTTTC TCTGTGGGCC CCTGGGCCTC GGCACAGGCC 
TCTGCTGAGC TGAAATCAGA GAAGGACACG GATGCTGGAT CACTCTATAA GTCCAGCATG 
ATGCCCACCA AACATGCCAA GGTCCTGTTC CGCGCGGGCA GCGGCAAGCC CCTGGCCGTG 
CTCTGCCTGA CGGTGGAGCT GCAGCCTCAC GTGGTAGACC AGGTCTTCCG CTTCCACCAC 
CCGGAGCTCA CCTTCCTGAA GAAGGCCATC CGCCTGCCGC CCTGGCACAC GCTTCCAGGT 
GCCCCTGTAG GGACTTCCAG TGAGGACCCC CCTTTCCACG TTCGATGTAG TGACCCGAAC 
ATCATTTGTG AGACACAGAA TGTGGGCCCC GGGGAGCCCC GTGACGTGTT CCTGAAGGTG 
GCCAGTGGCC CTAGCCCGGA GGTCAAGGAC TTCTTTGTCA CCATCTACGT AGACCGCTGG 
TTAGCAACAC CCGTCCAGAC CTGGCAGGTC CACCTGCACT CGCTGCAGCG AGTGGACGTC 
TCCTGTATCA CTGGCCAGAG GACCCGCCTG GCTCTCGTCC TCCGGGGGAC GCAGACAGTT 
AGGAGAGTGC AGGCTTTCAG CTCACACCCC CAGGAGCTGA CGACGGACCC CAGAGGTGTG 
TTCGTGCTGC CTCCTCACGG GGTGCAGGAC TTGCATGTGA GCGTCAGGCC CCGCAGGCCG 
GGCAGCCGCT TCGCGCACCT CCACCTGGTC GACGTGGACG CCCACCAGCT GGTGGCCTCG 
TGGCTCCTGT GTCTCTCCTG CCGGCCGCCT CTCATCTCCA AGGCCTTCGA GATAAAGCTG 
CCCACGGGGA GCGGGAGGGG CGCCAACAAG CGCATCACCT ACACCAACCC CTACCCCTCG 
CGGAGGGCCT ACCGCCTGCA CAGCGACCAC CCCCACCTGC TGCAGTTCAA GGAGGACGCC 
TTCCAGGTCG GGGGAGGCGA GACCTATACC ATCGGCCTGC GGTTTGCGTC CCGTCAGAGA 
GCTGGGGAGG AAGAGATCCT GATTTACATC AACGACCACG AAGACAAAAA CGAGGAGACC 
TTTTGCGTGA AGGTCATCTA CCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141602.1
CDS	41..4567
Translation	MIVPSPPVPSRVSIIAPCTLLFSVIFIGHNHLFCVLTCILCFCLPSDTFLEVNVVVCSVLQGLAQGNPRGPRDLGTGRMHDWHRIFDQNQLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLLEVDCHLRVSLFDVTYRHFFGRTWKSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLDLVISDCFREAGSPLDVGALEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVHHPAFAIVFQLEYVFNSPSGADGNAASLTSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASMSSEEVKQVESGTVQFQYSLSPEEHPDTPTRNASGPKAERRSFRKPPASPSSPPASPSQGLTAPQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLRLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLVGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRLKNVVQAQKLADVDSELAALLFTPPRPGARGPQGISQEGDAVRRRKLERMRAVRLRECGSEPGRRGLGVLAQQSLHAQHARDLQVIAAYRERTKAESIASALSMAITTQHTIHATLGTAEFFEFALRNPHNAQHTVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLYLRPRETAHIPFKFQTFSVGPWASAQASAELKSEKDTDAGSLYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQNVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPRRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPTGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFASRQRAGEEEILIYINDHEDKNEETFCVKVIYQ

Target ORF information:

RefSeq Version	XM_025285817.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis nephrocystin 4 (NPHP4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025285817.1

ORF Insert Sequence:

ATGATTGTGC CGTCGCCGCC AGTTCCAAGC CGGGTCTCCA TCATAGCTCC CTGCACCCTC 
CTCTTCTCTG TGATTTTTAT AGGTCACAAC CACCTCTTTT GTGTGCTAAC TTGTATCCTG 
TGTTTCTGCC TCCCTTCAGA TACATTCCTC GAGGTCAACG TGGTTGTCTG CAGTGTTCTC 
CAGGGCCTGG CACAGGGTAA TCCCAGAGGC CCCAGGGACC TGGGGACTGG CAGAATGCAC 
GACTGGCACA GGATCTTTGA CCAAAACCAG CTGGTCCCTC CGCACCCACA GAGAGCACGC 
CAGCCTCTGA AGGAATCGAC AGCGTTTCAG TGTGTCCTGA AGTGGCTCGA TGGCCCGCTA 
ATTAAGCAGG GGGTGCTGGA GCTGCTATTG GAGGTGGACT GCCACCTGCG CGTGTCCCTC 
TTCGATGTCA CCTATCGGCA CTTCTTTGGG AGGACGTGGA AGAGCTCGCC GAGGCCTGCA 
GAGCCGCTCA CCAGGCAGCC GGCCAGGATC GTCTTCAATG AGCCCTTGTA TTTTCACACG 
TCCTTGAACC ACCCTCATAT TGTGGCCGTG GTGGAGGTGG TCGTTGAAGG CAGGAGACGA 
GACGGGACCC TCCAGACTCT GTCCTGTGGG TTTGGAATTC TTCGGATCTT CAGCCACAAG 
CCGGAATCTC CAACTTCCAC TTCCCAGGAC AAACGGCTGA GACTGTATCA CGGGAGCCCG 
AGAGCCCTCC TCCACCCACT CCTTCAGGAC CCCATAGAAC AGAACAAGCA CATGACCCTC 
ATTGAGAACT GCAGCCTGCA GTACACGCTG AGGCCACACC CGCTTCTGGA GCCCGTGTTC 
CACCTTCTTC CCGAGAACCT GCTGGTGTCC GGTCTGCAGC AGATACCTGG TCTCCTTCCA 
GCTCACGGAG AGTCAGGAGA TGCCCTCCGG AAGCCCCGCC TGCAGAAGCC TCTGACGTGC 
TACCTGGATG ATCTGTTCTT CACGCTGTAT CCCTCGCTGG AGAAGTTCGA GGAGGAACTT 
CTGGATCTTG TGATCAGCGA TTGCTTCCGG GAGGCGGGCA GCCCTCTGGA TGTCGGCGCC 
TTGGAGATCC TGGAGAGGCG CCTGTGCGTG GGCGTGCACA ATGGTTTGGG GTTTGTGCAG 
CGGCCGCAGG TCCTCGTGCT GGTGCCCGAG CTGGAGGTGG CCTTGACACG CTCTGCCAGC 
TTCAGCCGGA AGGTCGGCTC CTCTTCCAAG GCCAGCTCTG GAAACCAGGC GCTGGTCCTG 
AGAAGCCGTC TCCGACTCCC TGAGATGGTC CATCACCCCG CGTTCGCCAT TGTCTTCCAG 
CTGGAGTACG TGTTTAACAG CCCCTCGGGG GCGGACGGCA ATGCAGCTTC ACTCACCTCC 
TTGTCCAGCC TGGCATGCAT GCACATGGTT CGCTGGGCTG TCTGGAACCC CTCGTTGGAA 
GCCAGCTCCG GACAGGTGAT GTTACCTCTC CAGGGTGGCA TCCAGTCCAA CCCTTCGCAC 
TGTCTGGTCT ATAAGGTACC CCCAGCCAGC ATGAGCTCTG AAGAGGTGAA GCAGGTGGAG 
TCAGGGACGG TCCAGTTCCA GTACTCGCTG AGCCCTGAAG AACACCCAGA CACACCCACA 
AGGAATGCAA GCGGCCCCAA GGCAGAGCGG CGGTCTTTTA GGAAGCCACC CGCGTCCCCT 
TCAAGTCCAC CAGCATCGCC GTCCCAGGGG CTCACCGCTC CCCAGGACTC ACCCATGGGA 
CCAGGGCTGT CGCTTTCCCA GCTGGCGGCT TCCCCACCAT CCCCAGCTCC ACGCCGCCGC 
TCGGCCAAAC CCTCATCACA GCCACCTGGG AGCCCTGTGC CCTCCCTGGT CCCAGGGGAG 
TTCCCCTTGG AGGGCAGGAT CTCTCACCTG GAGGCTGACC TGAGCCAGAC AGCCTTAGCC 
CTGGGAACGT CCGCTGCTGA CCACTTGCAG GAACTGCCTT TCACGCCTGT GCACGCCCCG 
ATCATTGCAG GAACCCAGAC CAGGAGCTCT GGAGGGAAGC TATCACGGGC GTCCCTGGCG 
CTGCTGCAGT CTGCTGGCTT TCCCGAGATT CTGGACGCCA ACAAGCAACC AGCTGAGGCT 
GTGAGTCCTA CAGACCCCGT GAAGTTCAGC CCTCAGAAGG AAGAATCAGA CTGTCTACAA 
GGCAATGAGA TAGTGCTGCA ATTTCTTGCC TTTAGCAGGG TGCCCCAGGA TGAGCAGGCA 
GCGCCATGGC CACAGACTGT GTACTTCACC TTCCAATTCT ACCGCTTCCC ACCCGAGACC 
ACACCACGAC TGCGGCTCGT GGAGCTGGCC GATGCTGGGA AAACCAGCTC TGCCTCCCTG 
TCGCACATCC TCGCCCAGAT CCAGAAGGAT GGCTCCTTTG ATGCGGGGTC TCCGGGCTTT 
CAGCGGAAGT ACCTTGTAGA CCCCGGGTTC CTGAGACCCG GGGAGCAGCG CTGGTTCGCC 
CGCTACCTGG CCATGCAGAC CCTGCAGATT GACGTCTGGG ATGGGGACTC CCTGCTTCTC 
GTCGGATCTG CTGCCGTTCA GCTGAAGCAC CTTCTCCGCC AGGGGCGGCC AGCCGTGCAG 
GTCTCGCACG AGCTGGAGGT TGTGGCGACT GAATATGAGC AGGACGCCAT GGTGGTGAGT 
GGAGACATGA CCAAGTTTGG CTGCGTCAGA CCCATCGACG TCCACACAGT GGTGAAGGGC 
CAGCTGCACT TGACATTGGC CAACGTGGGT CACTTGTGTG AACAGAGAGT AAGGAGTCCC 
AGTTCCCTGC CACCTTCCAG GTCGAGGGTC ATCTCAAATG ATGGAGTCGG CAACTTCATG 
GGAGGCAGCC TCCTGACACC CGGGGGCCCC AGATTGAAAA ATGTGGTCCA AGCCCAGAAG 
CTGGCTGATG TGGACAGCGA ACTGGCCGCC CTGCTCTTCA CACCCCCAAG ACCAGGGGCC 
CGGGGGCCCC AGGGCATCAG TCAAGAGGGG GATGCCGTCC GCAGGCGCAA GCTGGAGCGC 
ATGAGGGCCG TGCGGCTGCG GGAGTGTGGA TCGGAGCCGG GCCGCCGCGG GCTGGGCGTG 
CTGGCCCAGC AGAGCCTCCA TGCCCAGCAC GCCCGAGACC TGCAGGTCAT TGCCGCCTAC 
CGTGAGCGCA CGAAGGCGGA GAGCATCGCC AGTGCGCTGA GCATGGCCAT CACCACGCAG 
CACACCATCC ACGCCACCCT AGGCACTGCC GAGTTCTTCG AGTTCGCCCT GAGGAACCCC 
CACAACGCGC AGCACACGGT GACCATCGAG ATAGACAACC CCGAGCTCAG TCTCATCCTG 
GATGGTCAGG AGTGGCGGTA CTTCAAGGAC ATGGCTGACT TGCACACACC CCTGGAGGAG 
GACATGTTCC ACCTGCGGGG CAGCCTGGCC CCCCAACTCT ACCTGCGACC TCGGGAGACT 
GCCCACATCC CCTTCAAGTT CCAGACTTTC TCTGTGGGCC CCTGGGCCTC GGCACAGGCC 
TCTGCTGAGC TGAAATCAGA GAAGGACACG GATGCTGGAT CACTCTATAA GTCCAGCATG 
ATGCCCACCA AACATGCCAA GGTCCTGTTC CGCGCGGGCA GCGGCAAGCC CCTGGCCGTG 
CTCTGCCTGA CGGTGGAGCT GCAGCCTCAC GTGGTAGACC AGGTCTTCCG CTTCCACCAC 
CCGGAGCTCA CCTTCCTGAA GAAGGCCATC CGCCTGCCGC CCTGGCACAC GCTTCCAGGT 
GCCCCTGTAG GGACTTCCAG TGAGGACCCC CCTTTCCACG TTCGATGTAG TGACCCGAAC 
ATCATTTGTG AGACACAGAA TGTGGGCCCC GGGGAGCCCC GTGACGTGTT CCTGAAGGTG 
GCCAGTGGCC CTAGCCCGGA GGTCAAGGAC TTCTTTGTCA CCATCTACGT AGACCGCTGG 
TTAGCAACAC CCGTCCAGAC CTGGCAGGTC CACCTGCACT CGCTGCAGCG AGTGGACGTC 
TCCTGTATCA CTGGCCAGAG GACCCGCCTG GCTCTCGTCC TCCGGGGGAC GCAGACAGTT 
AGGAGAGTGC AGGCTTTCAG CTCACACCCC CAGGAGCTGA CGACGGACCC CAGAGGTGTG 
TTCGTGCTGC CTCCTCACGG GGTGCAGGAC TTGCATGTGA GCGTCAGGCC CCGCAGGCCG 
GGCAGCCGCT TCGCGCACCT CCACCTGGTC GACGTGGACG CCCACCAGCT GGTGGCCTCG 
TGGCTCCTGT GTCTCTCCTG CCGGCCGCCT CTCATCTCCA AGGCCTTCGA GATAAAGCTG 
CCCACGGGGA GCGGGAGGGG CGCCAACAAG CGCATCACCT ACACCAACCC CTACCCCTCG 
CGGAGGGCCT ACCGCCTGCA CAGCGACCAC CCCCACCTGC TGCAGTTCAA GGAGGACGCC 
TTCCAGGTCG GGGGAGGCGA GACCTATACC ATCGGCCTGC GGTTTGCGTC CCGTCAGAGA 
GCTGGGGAGG AAGAGATCCT GATTTACATC AACGACCACG AAGACAAAAA CGAGGAGACC 
TTTTGCGTGA AGGTCATCTA CCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NPHP4
Entrez Gene ID	102415509
Full Name	nephrocystin 4
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NPHP4 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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