PLEKHM2 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLEKHM2
Entrez Gene ID	102414752
Full Name	pleckstrin homology and RUN domain containing M2
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006072799.1	XP_006072861.1	pleckstrin homology domain-containing family M member 2
XM_025285664.1	XP_025141449.1	pleckstrin homology domain-containing family M member 2

Pathways from BioSystems
Gene Ontology

The following PLEKHM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLEKHM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu26990	XM_006072799.1 Latest version!	Bubalus bubalis pleckstrin homology domain containing, family M (with RUN domain) member 2 (PLEKHM2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu66096	XM_025285664.1 Latest version!	Bubalus bubalis pleckstrin homology and RUN domain containing M2 (PLEKHM2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLEKHM2 ( XM_006072799.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006072861.1 Bubalus bubalis pleckstrin homology domain containing, family M (with RUN domain) member 2 (PLEKHM2), mRNA.

Price & Availability↑

CloneID	OBu26990
Clone ID Related Accession (Same CDS sequence)	XM_006072799.1
Accession Version	XM_006072799.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology domain-containing family M member 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785503.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAACC AAGGAATGAG CATGAACAGA GTGCTTCACA TGACCAAGAC CATCCTTGTC 
ACGCAGGGGA CCTTGAACTT CTTGGAGAAA GGCAACAAAG CAGTGCTGGA CCGGATTCTG 
GAGAACATCT CGCTGTCGGT GAAGAAGTTG CAGAGCTACT TTGCTGCCTG TGAAGATGAG 
ACCCCTGCCA TCCGCAACCA CGACAAAGTC CTGCAGCGTC TGTGTGAGCA CTTGGACCAC 
GCTCTGCTCT ACGGGCTGCA GGACCTCTCA TCTGGTTACT GGGTGCTCGT GGTTCATTTC 
ACCAGGAGAG AGGCCGTCAA GCACATCGAG GTGCTACAGC ACGTGGCCAC CAACCTGGGG 
CGCAGCCGGG CCTGGCTGTA CCTGGCCCTC AACGAAAACT CCCTGGAGAG CTACCTGCGG 
CTGTTCCAGG AGAACCTGGG TCTCTTGCAC AAGTACTATG TCAAGAATGC CCTGGTGTGT 
AGCCATGACC ACCTGACCCT GTTCCTGACC TTGGTATCCG GGCTGGAGTT CATTCGGTTT 
GACCTGGACC TGGATGCCCC TTATTTAGAC CTGGCCCCCT ACATGCCCGA CTACTACAAG 
CCCCAGTACC TGCTGGACTT TGAAGACCGC CTCCCCAGCT CAGTTCACGG CTCAGACAGC 
CTCTCCCTCA ATTCCTTCAA CTCCGTCACC TCCACCACCC TGGAGTGGGA TGACAGCGCC 
ATCGCCCCGT CTAGTGAGGA TTATGATTTT GGAGATGTGT TTCCAGCAGT GCCGTCTGTA 
CCCAGCACAG ACTGGGAAGA CGGAGACCTC ACAGACACTG TCAGTGGCCC CCGCTCCACC 
ACCTCAGACC CAACCAGCAG CAAGGCTTCC ACCAAGAGTC CCACCCAGCG CCACAACCCC 
TTCAATGAGG ACCAGGCCGA GACTGTATCC TCCTCCGACA CCACCCCTGT GCACACAAGC 
TCTCAGGAGA AGGGGGAGTC CCACACCCCG GACCTACCGG ACACCTGCAC AGAGCTCGAG 
GTCATCAGGG TCACCAAGAA GAAGAAAACA GGCAAGAAGA AGAAGCCCAG ATCAGATGAG 
GAAGCAAGTC CACTCCACCC AGCCTCCACC CAGCACACGT GTGCCCGGCA GGGCAATGGC 
GACACCATGG TCAGCAGCCC AGGCCCTGGC CGGGGCTCCC CAGACACCAC CCTGGCCTCC 
CCGCCACAGG AGGGACAGGG GCCTGGCAGC ACAGTGGAGG GCAGCGAGCG CTCAGAGCCC 
GGCCAGGTGG GCCTGCTTAT CCCCGAGATG AAGGACACCT CCATGGAGCG CGTGGGGCAG 
CCCCTGAGTA AGGTCATCGA CCAGTTCAAC GGGCAGCTGG ACCCGGCCAC CTGGCGCTCC 
CACGTCGAGC CCCCGGACCA GTCCTTTCGG ACCGGCTCTC CCGGGGACGC CCCGGAGAGG 
CCGCCATTTT GCGACTTTAG TGAGGGGCTT CCAGCCCCAA TGGACTTCTA CTGCTTTACC 
GTTGAGAGTC CAAGTACTGT TACATCAGGT GGCGGCCACC ATGACCCTGC AGGGCCTGGC 
CAACCGCTGC ATGTTCCTGG TAGCCCTGCG GCTGCTGGCC AAGAAGAGGC GGGGGGAGGA 
GGAGGACCAG GACAGACGCC TCGGCCCTTA GAGGACACCC CGGGGGAGGC GCAGAAGCCA 
GAGGCCCAGG AACTGGACAC CCAGCTGCCC CAGGACGGTG AGGGGCTGGA GCCAGAGCCT 
GGGACCCAGG AGGCTCTTTG CAGACTCAAG GGAGACCAGC CCAGCCCCTG TCTGAGCAGC 
GCCGAGGACT CCGGGGTGGA GGAGGGCCAG GGGAGCCCTT CTGAGATGAC CCACTCGGCA 
GAGTTCAGAG TAGACAACAA TCACCTGCTC CTGCTGATGA TCCATGTGTT TCGAGAAAAC 
GAAGAGCAGC TCTTCAGAAT GATCCGGATG AGCACCGGGC ACATGGAGGG CAACCTGCAG 
CTGCTGTATG TGCTGCTCAC AGACTGCTAT GTCTACCTGC TCCGGAAAGG GGCCACAGAG 
AAGCCATACT TGGTGGAAGA GGCTGTTTCT TACAATGAGC TCGACTACGT GTCGGTGGGC 
CTCGACCAAC AGACGGTGAA GCTGGTGTGC ACCAACCGCA GGAAGCAGTT TCTGCTGGAC 
ACAGCGGACG TGGCCCTGGC CGAGTTCTTC TTGGCTTCTT TGAAGTCAGC CATGATCAAG 
GGCTGTCGGG AACCCCCCTA CCCCAGCATC CTGACTGATG CCACCATGGA GAAGCTGGCT 
CTGGCTAAAT TTGTGGCCCA GGAATCCAAG TGTGAGGCGA CTGCTGTTAC TGTGCGCTTC 
TATGGGCTTG TGCACTGGGA GGACCCCCAG GACGAGTTCC TGGGCCCCAC CCCCTGCCAC 
TGCTCACCCC CTGAGGGTAC CATCACCAAA GAAGGCATGC TGCATTACAA GGCGGGTACC 
TCCTACCTGG GCAAGGAGCA CTGGAAGACC TGCTTCGTAG TGCTCAGCAA TGGGATCCTC 
TACCAATATC CTGACCGCAC CGACGTGACG CCCCTGCTCT CGGTGAACAT GGGGGGGGAG 
CAGTGCGGTG GCTGTCGGAG ATCCAACACC ACGGATCGGC CCCACGCCTT CCAGGTCATC 
CTCGCCGACC GGCCCTGCCT GGAGCTGAGC GCCGACAGCG AGGCTGAGAT GGCCGACTGG 
ATGCAGCACC TGTGCCAGGC CGTGTCCAAG GGGGTCATCC CCCAGGGGGT AGCTCCCAGC 
CCTTGCATCC CTTGCTGCCT GGTAATCACC GATGACCGCC TCTTCACATG CCACGAGGAT 
TGCCAGACCA GCTTCTTCCG CTCCCTGGGT ACTGCCAAGC TGGCCGACAT CAGCGCTGTG 
TCCACTGAAC CGGGCAAGGA GTACTGCCTC TTGGAGTTCT CCCAGGACAG CGCACAGCCC 
CTCCCACCCT GGGTCGTCTA CCTGAGCTGC ACACCTGAAC TGGACCGATT CCTATCTGCA 
CTGAACTCGG GGTGGAAAAC CATCTACCAG GTGGACCTCC CCCATAAGGC CATCCAGGAA 
GCCTCCAGGA AGAAGTTCGA GGATGCCCTG AGCCTCATCC ACAGTGCCTG GCAGCGGAGC 
GACAGCCTGT GCCGGGGCAG AGCCTCCCGG GACCCCTGGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006072861.1
CDS	1..3165
Translation	MKNQGMSMNRVLHMTKTILVTQGTLNFLEKGNKAVLDRILENISLSVKKLQSYFAACEDETPAIRNHDKVLQRLCEHLDHALLYGLQDLSSGYWVLVVHFTRREAVKHIEVLQHVATNLGRSRAWLYLALNENSLESYLRLFQENLGLLHKYYVKNALVCSHDHLTLFLTLVSGLEFIRFDLDLDAPYLDLAPYMPDYYKPQYLLDFEDRLPSSVHGSDSLSLNSFNSVTSTTLEWDDSAIAPSSEDYDFGDVFPAVPSVPSTDWEDGDLTDTVSGPRSTTSDPTSSKASTKSPTQRHNPFNEDQAETVSSSDTTPVHTSSQEKGESHTPDLPDTCTELEVIRVTKKKKTGKKKKPRSDEEASPLHPASTQHTCARQGNGDTMVSSPGPGRGSPDTTLASPPQEGQGPGSTVEGSERSEPGQVGLLIPEMKDTSMERVGQPLSKVIDQFNGQLDPATWRSHVEPPDQSFRTGSPGDAPERPPFCDFSEGLPAPMDFYCFTVESPSTVTSGGGHHDPAGPGQPLHVPGSPAAAGQEEAGGGGGPGQTPRPLEDTPGEAQKPEAQELDTQLPQDGEGLEPEPGTQEALCRLKGDQPSPCLSSAEDSGVEEGQGSPSEMTHSAEFRVDNNHLLLLMIHVFRENEEQLFRMIRMSTGHMEGNLQLLYVLLTDCYVYLLRKGATEKPYLVEEAVSYNELDYVSVGLDQQTVKLVCTNRRKQFLLDTADVALAEFFLASLKSAMIKGCREPPYPSILTDATMEKLALAKFVAQESKCEATAVTVRFYGLVHWEDPQDEFLGPTPCHCSPPEGTITKEGMLHYKAGTSYLGKEHWKTCFVVLSNGILYQYPDRTDVTPLLSVNMGGEQCGGCRRSNTTDRPHAFQVILADRPCLELSADSEAEMADWMQHLCQAVSKGVIPQGVAPSPCIPCCLVITDDRLFTCHEDCQTSFFRSLGTAKLADISAVSTEPGKEYCLLEFSQDSAQPLPPWVVYLSCTPELDRFLSALNSGWKTIYQVDLPHKAIQEASRKKFEDALSLIHSAWQRSDSLCRGRASRDPWC

Target ORF information:

RefSeq Version	XM_006072799.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology domain containing, family M (with RUN domain) member 2 (PLEKHM2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006072799.1

ORF Insert Sequence:

ATGAAGAACC AAGGAATGAG CATGAACAGA GTGCTTCACA TGACCAAGAC CATCCTTGTC 
ACGCAGGGGA CCTTGAACTT CTTGGAGAAA GGCAACAAAG CAGTGCTGGA CCGGATTCTG 
GAGAACATCT CGCTGTCGGT GAAGAAGTTG CAGAGCTACT TTGCTGCCTG TGAAGATGAG 
ACCCCTGCCA TCCGCAACCA CGACAAAGTC CTGCAGCGTC TGTGTGAGCA CTTGGACCAC 
GCTCTGCTCT ACGGGCTGCA GGACCTCTCA TCTGGTTACT GGGTGCTCGT GGTTCATTTC 
ACCAGGAGAG AGGCCGTCAA GCACATCGAG GTGCTACAGC ACGTGGCCAC CAACCTGGGG 
CGCAGCCGGG CCTGGCTGTA CCTGGCCCTC AACGAAAACT CCCTGGAGAG CTACCTGCGG 
CTGTTCCAGG AGAACCTGGG TCTCTTGCAC AAGTACTATG TCAAGAATGC CCTGGTGTGT 
AGCCATGACC ACCTGACCCT GTTCCTGACC TTGGTATCCG GGCTGGAGTT CATTCGGTTT 
GACCTGGACC TGGATGCCCC TTATTTAGAC CTGGCCCCCT ACATGCCCGA CTACTACAAG 
CCCCAGTACC TGCTGGACTT TGAAGACCGC CTCCCCAGCT CAGTTCACGG CTCAGACAGC 
CTCTCCCTCA ATTCCTTCAA CTCCGTCACC TCCACCACCC TGGAGTGGGA TGACAGCGCC 
ATCGCCCCGT CTAGTGAGGA TTATGATTTT GGAGATGTGT TTCCAGCAGT GCCGTCTGTA 
CCCAGCACAG ACTGGGAAGA CGGAGACCTC ACAGACACTG TCAGTGGCCC CCGCTCCACC 
ACCTCAGACC CAACCAGCAG CAAGGCTTCC ACCAAGAGTC CCACCCAGCG CCACAACCCC 
TTCAATGAGG ACCAGGCCGA GACTGTATCC TCCTCCGACA CCACCCCTGT GCACACAAGC 
TCTCAGGAGA AGGGGGAGTC CCACACCCCG GACCTACCGG ACACCTGCAC AGAGCTCGAG 
GTCATCAGGG TCACCAAGAA GAAGAAAACA GGCAAGAAGA AGAAGCCCAG ATCAGATGAG 
GAAGCAAGTC CACTCCACCC AGCCTCCACC CAGCACACGT GTGCCCGGCA GGGCAATGGC 
GACACCATGG TCAGCAGCCC AGGCCCTGGC CGGGGCTCCC CAGACACCAC CCTGGCCTCC 
CCGCCACAGG AGGGACAGGG GCCTGGCAGC ACAGTGGAGG GCAGCGAGCG CTCAGAGCCC 
GGCCAGGTGG GCCTGCTTAT CCCCGAGATG AAGGACACCT CCATGGAGCG CGTGGGGCAG 
CCCCTGAGTA AGGTCATCGA CCAGTTCAAC GGGCAGCTGG ACCCGGCCAC CTGGCGCTCC 
CACGTCGAGC CCCCGGACCA GTCCTTTCGG ACCGGCTCTC CCGGGGACGC CCCGGAGAGG 
CCGCCATTTT GCGACTTTAG TGAGGGGCTT CCAGCCCCAA TGGACTTCTA CTGCTTTACC 
GTTGAGAGTC CAAGTACTGT TACATCAGGT GGCGGCCACC ATGACCCTGC AGGGCCTGGC 
CAACCGCTGC ATGTTCCTGG TAGCCCTGCG GCTGCTGGCC AAGAAGAGGC GGGGGGAGGA 
GGAGGACCAG GACAGACGCC TCGGCCCTTA GAGGACACCC CGGGGGAGGC GCAGAAGCCA 
GAGGCCCAGG AACTGGACAC CCAGCTGCCC CAGGACGGTG AGGGGCTGGA GCCAGAGCCT 
GGGACCCAGG AGGCTCTTTG CAGACTCAAG GGAGACCAGC CCAGCCCCTG TCTGAGCAGC 
GCCGAGGACT CCGGGGTGGA GGAGGGCCAG GGGAGCCCTT CTGAGATGAC CCACTCGGCA 
GAGTTCAGAG TAGACAACAA TCACCTGCTC CTGCTGATGA TCCATGTGTT TCGAGAAAAC 
GAAGAGCAGC TCTTCAGAAT GATCCGGATG AGCACCGGGC ACATGGAGGG CAACCTGCAG 
CTGCTGTATG TGCTGCTCAC AGACTGCTAT GTCTACCTGC TCCGGAAAGG GGCCACAGAG 
AAGCCATACT TGGTGGAAGA GGCTGTTTCT TACAATGAGC TCGACTACGT GTCGGTGGGC 
CTCGACCAAC AGACGGTGAA GCTGGTGTGC ACCAACCGCA GGAAGCAGTT TCTGCTGGAC 
ACAGCGGACG TGGCCCTGGC CGAGTTCTTC TTGGCTTCTT TGAAGTCAGC CATGATCAAG 
GGCTGTCGGG AACCCCCCTA CCCCAGCATC CTGACTGATG CCACCATGGA GAAGCTGGCT 
CTGGCTAAAT TTGTGGCCCA GGAATCCAAG TGTGAGGCGA CTGCTGTTAC TGTGCGCTTC 
TATGGGCTTG TGCACTGGGA GGACCCCCAG GACGAGTTCC TGGGCCCCAC CCCCTGCCAC 
TGCTCACCCC CTGAGGGTAC CATCACCAAA GAAGGCATGC TGCATTACAA GGCGGGTACC 
TCCTACCTGG GCAAGGAGCA CTGGAAGACC TGCTTCGTAG TGCTCAGCAA TGGGATCCTC 
TACCAATATC CTGACCGCAC CGACGTGACG CCCCTGCTCT CGGTGAACAT GGGGGGGGAG 
CAGTGCGGTG GCTGTCGGAG ATCCAACACC ACGGATCGGC CCCACGCCTT CCAGGTCATC 
CTCGCCGACC GGCCCTGCCT GGAGCTGAGC GCCGACAGCG AGGCTGAGAT GGCCGACTGG 
ATGCAGCACC TGTGCCAGGC CGTGTCCAAG GGGGTCATCC CCCAGGGGGT AGCTCCCAGC 
CCTTGCATCC CTTGCTGCCT GGTAATCACC GATGACCGCC TCTTCACATG CCACGAGGAT 
TGCCAGACCA GCTTCTTCCG CTCCCTGGGT ACTGCCAAGC TGGCCGACAT CAGCGCTGTG 
TCCACTGAAC CGGGCAAGGA GTACTGCCTC TTGGAGTTCT CCCAGGACAG CGCACAGCCC 
CTCCCACCCT GGGTCGTCTA CCTGAGCTGC ACACCTGAAC TGGACCGATT CCTATCTGCA 
CTGAACTCGG GGTGGAAAAC CATCTACCAG GTGGACCTCC CCCATAAGGC CATCCAGGAA 
GCCTCCAGGA AGAAGTTCGA GGATGCCCTG AGCCTCATCC ACAGTGCCTG GCAGCGGAGC 
GACAGCCTGT GCCGGGGCAG AGCCTCCCGG GACCCCTGGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHM2 ( XM_025285664.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141449.1 Bubalus bubalis pleckstrin homology and RUN domain containing M2 (PLEKHM2), mRNA.

Price & Availability↑

CloneID	OBu66096
Clone ID Related Accession (Same CDS sequence)	XM_025285664.1
Accession Version	XM_025285664.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology domain-containing family M member 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGC GGGAGGTGAA GGACCGGATT CTGGAGAACA TCTCGCTGTC GGTGAAGAAG 
TTGCAGAGCT ACTTTGCTGC CTGTGAAGAT GAGACCCCTG CCATCCGCAA CCACGACAAA 
GTCCTGCAGC GTCTGTGTGA GCACTTGGAC CACGCTCTGC TCTACGGGCT GCAGGACCTC 
TCATCTGGTT ACTGGGTGCT CGTGGTTCAT TTCACCAGGA GAGAGGCCGT CAAGCACATC 
GAGGTGCTAC AGCACGTGGC CACCAACCTG GGGCGCAGCC GGGCCTGGCT GTACCTGGCC 
CTCAACGAAA ACTCCCTGGA GAGCTACCTG CGGCTGTTCC AGGAGAACCT GGGTCTCTTG 
CACAAGTACT ATGTCAAGAA TGCCCTGGTG TGTAGCCATG ACCACCTGAC CCTGTTCCTG 
ACCTTGGTAT CCGGGCTGGA GTTCATTCGG TTTGACCTGG ACCTGGATGC CCCTTATTTA 
GACCTGGCCC CCTACATGCC CGACTACTAC AAGCCCCAGT ACCTGCTGGA CTTTGAAGAC 
CGCCTCCCCA GCTCAGTTCA CGGCTCAGAC AGCCTCTCCC TCAATTCCTT CAACTCCGTC 
ACCTCCACCA CCCTGGAGTG GGATGACAGC GCCATCGCCC CGTCTAGTGA GGATTATGAT 
TTTGGAGATG TGTTTCCAGC AGTGCCGTCT GTACCCAGCA CAGACTGGGA AGACGGAGAC 
CTCACAGACA CTGTCAGTGG CCCCCGCTCC ACCACCTCAG ACCCAACCAG CAGCAAGGCT 
TCCACCAAGA GTCCCACCCA GCGCCACAAC CCCTTCAATG AGGACCAGGC CGAGACTGTA 
TCCTCCTCCG ACACCACCCC TGTGCACACA AGCTCTCAGG AGAAGGGGGA GTCCCACACC 
CCGGACCTAC CGGACACCTG CACAGAGCTC GAGGTCATCA GGGTCACCAA GAAGAAGAAA 
ACAGGCAAGA AGAAGAAGCC CAGATCAGAT GAGGAAGCAA GTCCACTCCA CCCAGCCTCC 
ACCCAGCACA CGTGTGCCCG GCAGGGCAAT GGCGACACCA TGGTCAGCAG CCCAGGCCCT 
GGCCGGGGCT CCCCAGACAC CACCCTGGCC TCCCCGCCAC AGGAGGGACA GGGGCCTGGC 
AGCACAGTGG AGGGCAGCGA GCGCTCAGAG CCCGGCCAGG TGGGCCTGCT TATCCCCGAG 
ATGAAGGACA CCTCCATGGA GCGCGTGGGG CAGCCCCTGA GTAAGGTCAT CGACCAGTTC 
AACGGGCAGC TGGACCCGGC CACCTGGCGC TCCCACGTCG AGCCCCCGGA CCAGTCCTTT 
CGGACCGGCT CTCCCGGGGA CGCCCCGGAG AGGCCGCCAT TTTGCGACTT TAGTGAGGGG 
CTTCCAGCCC CAATGGACTT CTACTGCTTT ACCGTCGAGA GTCCAAGTAC TGTTACATCA 
GGTGGCGGCC ACCATGACCC TGCAGGGCCT GGCCAACCGC TGCATGTTCC TGGTAGCCCT 
GCGGCTGCTG GCCAAGAAGA GGCGGGGGGA GGAGGAGGAC CAGGACAGAC GCCTCGGCCC 
TTAGAGGACA CCCCGGGGGA GGCGCAGAAG CCAGAGGCCC AGGAACTGGA CACCCAGCTG 
CCCCAGGACG GTGAGGGGCT GGAGCCAGAG CCTGGGACCC AGGAGGCTCT TTGCAGACTC 
AAGGGAGACC AGCCCAGCCC CTGTCTGAGC AGCGCCGAGG ACTCCGGGGT GGAGGAGGGC 
CAGGGGAGCC CTTCTGAGAT GACCCACTCG GCAGAGTTCA GAGTAGACAA CAATCACCTG 
CTCCTGCTGA TGATCCATGT GTTTCGAGAA AACGAAGAGC AGCTCTTCAG AATGATCCGG 
ATGAGCACCG GGCACATGGA GGGCAACCTG CAGCTGCTGT ATGTGCTGCT CACAGACTGC 
TATGTCTACC TGCTCCGGAA AGGGGCCACA GAGAAGCCAT ACTTGGTGGA AGAGGCTGTT 
TCTTACAATG AGCTCGACTA CGTGTCGGTG GGCCTCGACC AACAGACGGT GAAGCTGGTG 
TGCACCAACC GCAGGAAGCA GTTTCTGCTG GACACAGCGG ACGTGGCCCT GGCCGAGTTC 
TTCTTGGCTT CTTTGAAGTC AGCCATGATC AAGGGCTGTC GGGAACCCCC CTACCCCAGC 
ATCCTGACTG ATGCCACCAT GGAGAAGCTG GCTCTGGCTA AATTTGTGGC CCAGGAATCC 
AAGTGTGAGG TAAGTGCTGT TACTGTGCGC TTCTATGGGC TTGTGCACTG GGAGGACCCC 
CAGGACGAGT TCCTGGGCCC CACCCCCTGC CACTGCTCAC CCCCTGAGGG TACCATCACC 
AAAGAAGGCA TGCTGCATTA CAAGGCGGGT ACCTCCTACC TGGGCAAGGA GCACTGGAAG 
ACCTGCTTTG TAGTGCTCAG CAATGGGATC CTCTACCAAT ATCCTGACCG CACCGACGTG 
ACGCCCCTGC TCTCGGTGAA CATGGGGGGG GAGCAGTGCG GTGGCTGTCG GAGATCCAAC 
ACCACGGATC GGCCCCACGC CTTCCAGGTC ATCCTCGCCG ACCGGCCCTG CCTGGAGCTG 
AGCGCCGACA GCGAGGCTGA GATGGCCGAC TGGATGCAGC ACCTGTGCCA GGCCGTGTCC 
AAGGGGGTCA TCCCCCAGGG GGTAGCTCCC AGCCCTTGCA TCCCTTGCTG CCTGGTAATC 
ACCGATGACC GCCTCTTCAC ATGCCACGAG GATTGCCAGA CCAGCTTCTT CCGCTCCCTG 
GGTACTGCCA AGCTGGCCGA CATCAGCGCT GTGTCCACTG AACCGGGCAA GGAGTACTGC 
CTCTTGGAGT TCTCCCAGGA CAGCGCACAG CCCCTCCCAC CCTGGGTCGT CTACCTGAGC 
TGCACACCTG AACTGGACCG ATTCCTATCT GCACTGAACT CGGGGTGGAA AACCATCTAC 
CAGGTGGACC TCCCCCATAA GGCCATCCAG GAAGCCTCCA GGAAGAAGTT CGAGGATGCC 
CTGAGCCTCA TCCACAGTGC CTGGCAGCGG AGCGACAGCC TGTGCCGGGG CAGAGCCTCC 
CGGGACCCCT GGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141449.1
CDS	91..3168
Translation	MEPREVKDRILENISLSVKKLQSYFAACEDETPAIRNHDKVLQRLCEHLDHALLYGLQDLSSGYWVLVVHFTRREAVKHIEVLQHVATNLGRSRAWLYLALNENSLESYLRLFQENLGLLHKYYVKNALVCSHDHLTLFLTLVSGLEFIRFDLDLDAPYLDLAPYMPDYYKPQYLLDFEDRLPSSVHGSDSLSLNSFNSVTSTTLEWDDSAIAPSSEDYDFGDVFPAVPSVPSTDWEDGDLTDTVSGPRSTTSDPTSSKASTKSPTQRHNPFNEDQAETVSSSDTTPVHTSSQEKGESHTPDLPDTCTELEVIRVTKKKKTGKKKKPRSDEEASPLHPASTQHTCARQGNGDTMVSSPGPGRGSPDTTLASPPQEGQGPGSTVEGSERSEPGQVGLLIPEMKDTSMERVGQPLSKVIDQFNGQLDPATWRSHVEPPDQSFRTGSPGDAPERPPFCDFSEGLPAPMDFYCFTVESPSTVTSGGGHHDPAGPGQPLHVPGSPAAAGQEEAGGGGGPGQTPRPLEDTPGEAQKPEAQELDTQLPQDGEGLEPEPGTQEALCRLKGDQPSPCLSSAEDSGVEEGQGSPSEMTHSAEFRVDNNHLLLLMIHVFRENEEQLFRMIRMSTGHMEGNLQLLYVLLTDCYVYLLRKGATEKPYLVEEAVSYNELDYVSVGLDQQTVKLVCTNRRKQFLLDTADVALAEFFLASLKSAMIKGCREPPYPSILTDATMEKLALAKFVAQESKCEVSAVTVRFYGLVHWEDPQDEFLGPTPCHCSPPEGTITKEGMLHYKAGTSYLGKEHWKTCFVVLSNGILYQYPDRTDVTPLLSVNMGGEQCGGCRRSNTTDRPHAFQVILADRPCLELSADSEAEMADWMQHLCQAVSKGVIPQGVAPSPCIPCCLVITDDRLFTCHEDCQTSFFRSLGTAKLADISAVSTEPGKEYCLLEFSQDSAQPLPPWVVYLSCTPELDRFLSALNSGWKTIYQVDLPHKAIQEASRKKFEDALSLIHSAWQRSDSLCRGRASRDPWC

Target ORF information:

RefSeq Version	XM_025285664.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology and RUN domain containing M2 (PLEKHM2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025285664.1

ORF Insert Sequence:

ATGGAGCCGC GGGAGGTGAA GGACCGGATT CTGGAGAACA TCTCGCTGTC GGTGAAGAAG 
TTGCAGAGCT ACTTTGCTGC CTGTGAAGAT GAGACCCCTG CCATCCGCAA CCACGACAAA 
GTCCTGCAGC GTCTGTGTGA GCACTTGGAC CACGCTCTGC TCTACGGGCT GCAGGACCTC 
TCATCTGGTT ACTGGGTGCT CGTGGTTCAT TTCACCAGGA GAGAGGCCGT CAAGCACATC 
GAGGTGCTAC AGCACGTGGC CACCAACCTG GGGCGCAGCC GGGCCTGGCT GTACCTGGCC 
CTCAACGAAA ACTCCCTGGA GAGCTACCTG CGGCTGTTCC AGGAGAACCT GGGTCTCTTG 
CACAAGTACT ATGTCAAGAA TGCCCTGGTG TGTAGCCATG ACCACCTGAC CCTGTTCCTG 
ACCTTGGTAT CCGGGCTGGA GTTCATTCGG TTTGACCTGG ACCTGGATGC CCCTTATTTA 
GACCTGGCCC CCTACATGCC CGACTACTAC AAGCCCCAGT ACCTGCTGGA CTTTGAAGAC 
CGCCTCCCCA GCTCAGTTCA CGGCTCAGAC AGCCTCTCCC TCAATTCCTT CAACTCCGTC 
ACCTCCACCA CCCTGGAGTG GGATGACAGC GCCATCGCCC CGTCTAGTGA GGATTATGAT 
TTTGGAGATG TGTTTCCAGC AGTGCCGTCT GTACCCAGCA CAGACTGGGA AGACGGAGAC 
CTCACAGACA CTGTCAGTGG CCCCCGCTCC ACCACCTCAG ACCCAACCAG CAGCAAGGCT 
TCCACCAAGA GTCCCACCCA GCGCCACAAC CCCTTCAATG AGGACCAGGC CGAGACTGTA 
TCCTCCTCCG ACACCACCCC TGTGCACACA AGCTCTCAGG AGAAGGGGGA GTCCCACACC 
CCGGACCTAC CGGACACCTG CACAGAGCTC GAGGTCATCA GGGTCACCAA GAAGAAGAAA 
ACAGGCAAGA AGAAGAAGCC CAGATCAGAT GAGGAAGCAA GTCCACTCCA CCCAGCCTCC 
ACCCAGCACA CGTGTGCCCG GCAGGGCAAT GGCGACACCA TGGTCAGCAG CCCAGGCCCT 
GGCCGGGGCT CCCCAGACAC CACCCTGGCC TCCCCGCCAC AGGAGGGACA GGGGCCTGGC 
AGCACAGTGG AGGGCAGCGA GCGCTCAGAG CCCGGCCAGG TGGGCCTGCT TATCCCCGAG 
ATGAAGGACA CCTCCATGGA GCGCGTGGGG CAGCCCCTGA GTAAGGTCAT CGACCAGTTC 
AACGGGCAGC TGGACCCGGC CACCTGGCGC TCCCACGTCG AGCCCCCGGA CCAGTCCTTT 
CGGACCGGCT CTCCCGGGGA CGCCCCGGAG AGGCCGCCAT TTTGCGACTT TAGTGAGGGG 
CTTCCAGCCC CAATGGACTT CTACTGCTTT ACCGTCGAGA GTCCAAGTAC TGTTACATCA 
GGTGGCGGCC ACCATGACCC TGCAGGGCCT GGCCAACCGC TGCATGTTCC TGGTAGCCCT 
GCGGCTGCTG GCCAAGAAGA GGCGGGGGGA GGAGGAGGAC CAGGACAGAC GCCTCGGCCC 
TTAGAGGACA CCCCGGGGGA GGCGCAGAAG CCAGAGGCCC AGGAACTGGA CACCCAGCTG 
CCCCAGGACG GTGAGGGGCT GGAGCCAGAG CCTGGGACCC AGGAGGCTCT TTGCAGACTC 
AAGGGAGACC AGCCCAGCCC CTGTCTGAGC AGCGCCGAGG ACTCCGGGGT GGAGGAGGGC 
CAGGGGAGCC CTTCTGAGAT GACCCACTCG GCAGAGTTCA GAGTAGACAA CAATCACCTG 
CTCCTGCTGA TGATCCATGT GTTTCGAGAA AACGAAGAGC AGCTCTTCAG AATGATCCGG 
ATGAGCACCG GGCACATGGA GGGCAACCTG CAGCTGCTGT ATGTGCTGCT CACAGACTGC 
TATGTCTACC TGCTCCGGAA AGGGGCCACA GAGAAGCCAT ACTTGGTGGA AGAGGCTGTT 
TCTTACAATG AGCTCGACTA CGTGTCGGTG GGCCTCGACC AACAGACGGT GAAGCTGGTG 
TGCACCAACC GCAGGAAGCA GTTTCTGCTG GACACAGCGG ACGTGGCCCT GGCCGAGTTC 
TTCTTGGCTT CTTTGAAGTC AGCCATGATC AAGGGCTGTC GGGAACCCCC CTACCCCAGC 
ATCCTGACTG ATGCCACCAT GGAGAAGCTG GCTCTGGCTA AATTTGTGGC CCAGGAATCC 
AAGTGTGAGG TAAGTGCTGT TACTGTGCGC TTCTATGGGC TTGTGCACTG GGAGGACCCC 
CAGGACGAGT TCCTGGGCCC CACCCCCTGC CACTGCTCAC CCCCTGAGGG TACCATCACC 
AAAGAAGGCA TGCTGCATTA CAAGGCGGGT ACCTCCTACC TGGGCAAGGA GCACTGGAAG 
ACCTGCTTTG TAGTGCTCAG CAATGGGATC CTCTACCAAT ATCCTGACCG CACCGACGTG 
ACGCCCCTGC TCTCGGTGAA CATGGGGGGG GAGCAGTGCG GTGGCTGTCG GAGATCCAAC 
ACCACGGATC GGCCCCACGC CTTCCAGGTC ATCCTCGCCG ACCGGCCCTG CCTGGAGCTG 
AGCGCCGACA GCGAGGCTGA GATGGCCGAC TGGATGCAGC ACCTGTGCCA GGCCGTGTCC 
AAGGGGGTCA TCCCCCAGGG GGTAGCTCCC AGCCCTTGCA TCCCTTGCTG CCTGGTAATC 
ACCGATGACC GCCTCTTCAC ATGCCACGAG GATTGCCAGA CCAGCTTCTT CCGCTCCCTG 
GGTACTGCCA AGCTGGCCGA CATCAGCGCT GTGTCCACTG AACCGGGCAA GGAGTACTGC 
CTCTTGGAGT TCTCCCAGGA CAGCGCACAG CCCCTCCCAC CCTGGGTCGT CTACCTGAGC 
TGCACACCTG AACTGGACCG ATTCCTATCT GCACTGAACT CGGGGTGGAA AACCATCTAC 
CAGGTGGACC TCCCCCATAA GGCCATCCAG GAAGCCTCCA GGAAGAAGTT CGAGGATGCC 
CTGAGCCTCA TCCACAGTGC CTGGCAGCGG AGCGACAGCC TGTGCCGGGG CAGAGCCTCC 
CGGGACCCCT GGTGCTGA