ORF » Species Summary » Bubalus bubalis » WEE1 cDNA ORF clone

WEE1 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following WEE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WEE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu09465 XM_006051581.1
Latest version!		 Bubalus bubalis WEE1 G2 checkpoint kinase (WEE1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OBu50622 XM_006051581.2
Latest version!		 Bubalus bubalis WEE1 G2 checkpoint kinase (WEE1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBu50621 XM_006051580.2
Latest version!		 Bubalus bubalis WEE1 G2 checkpoint kinase (WEE1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBu09464 XM_006051580.1
Latest version!		 Bubalus bubalis WEE1 G2 checkpoint kinase (WEE1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    WEE1 ( XM_006051581.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006051643.1 Bubalus bubalis WEE1 G2 checkpoint kinase (WEE1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OBu09465
    Clone ID Related Accession (Same CDS sequence) XM_006051581.1
    Accession Version XM_006051581.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product wee1-like protein kinase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784387.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGATACAG AAAAATCAGG AAAAAGGGAA TTTGATGTTC GACAGACTCC TCAAGTGAAT 
    ATTAATCCTT TTACTCCAGA TTCTCTGTTA CTGCATTCCT CAGGACAGTG TCGTAGAAGA 
    AAGAGAGCAT ATTGGAATGA TTCCTGTGGT GAAGACATGG AAGCCAGTGA TTATGAGTTT 
    GAAGATGAAA CAAGACCTGC TAAAAGAATT ACAATTACTG AAAGCAATAT GAAGTCTCGA 
    TATACAACAG AATTTCATGA GCTAGAGAAG ATTGGTTCTG GAGAATTTGG TTCTGTGTTT 
    AAATGTGTGA AGAGGCTGGA TGGATGCGTT TATGCCATAA AGCGATCAAA AAAGCCATTG 
    GCTGGCTCTG TTGATGAGCA GAATGCTTTG AGAGAAGTAT ATGCTCACGC AGTGCTTGGG 
    CAACATTCTC ATGTAGTTCG ATACTTCTCT GCCTGGGCAG AAGATGATCA TATGCTTATA 
    CAGAATGAAT ATTGTAACGG TGGCAGTTTA GCCGATGCCA TAAGTGAAAA CTACAGAAGT 
    ATGAGTTACT TTACAGAAGC AGAGTTGAAG GATCTCCTCT TACAAGTTGG CCGGGGCTTG 
    AAGTATATTC ATTCAATGTC TTTAGTTCAC ATGGATATAA AGCCTAGTAA TATTTTCATA 
    TCTCGAACTT CAATCCCAAA TGCTGCCTCT GAAGAAGGAG ATGAAGATGA CTGGGCATCC 
    AACAAAGTTA TGTTTAAAAT AGGTGACCTT GGACATGTAA CAAGGATCTC TAGTCCACAA 
    GTTGAAGAGG GTGATAGCCG TTTTCTTGCA AATGAAGTTT TACAGGAGAA CTATACTCAC 
    CTACCAAAAG CAGATATTTT TGCCCTTGCC CTCACAGTGG TATGTGCTGC TGGTGCTGAG 
    CCTCTTCCCA GAAATGGAGA CCAATGGCAT GAAATTAGAC AGGGTAGATT ACCTCGGATT 
    CCACAAGTGC TTTCCCAAGA ATTTACAGAG TTGCTAAAAG TTATGATTCA TCCAGATCCA 
    GAGAGAAGAC CTTCAGCAAT GGCACTGGTA AAGCATTCAG TATTACTGTC TGCTTCAAGG 
    AAGAGTGCAG AACAATTACG AATAGAATTG AATGCTGAAA AGTTCAAAAA TTCACTTTTG 
    CAGAAAGAAC TCAAGAAAGC CCAGATGGCA AAAGCTGCAG CTGAGGAAAG AGCACTCTTC 
    ACTGATCGGA TGGCCACCAG GTCCACCACC CAGAGTAATA GAACATCTCG ACTTATTGGA 
    AAGAAAATGA ACCGCTCTGT CAGCCTTACC ATATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006051643.1
    CDS590..1888
    Translation

    Target ORF information:

    RefSeq Version XM_006051581.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis WEE1 G2 checkpoint kinase (WEE1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006051581.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGATACAG AAAAATCAGG AAAAAGGGAA TTTGATGTTC GACAGACTCC TCAAGTGAAT 
    ATTAATCCTT TTACTCCAGA TTCTCTGTTA CTGCATTCCT CAGGACAGTG TCGTAGAAGA 
    AAGAGAGCAT ATTGGAATGA TTCCTGTGGT GAAGACATGG AAGCCAGTGA TTATGAGTTT 
    GAAGATGAAA CAAGACCTGC TAAAAGAATT ACAATTACTG AAAGCAATAT GAAGTCTCGA 
    TATACAACAG AATTTCATGA GCTAGAGAAG ATTGGTTCTG GAGAATTTGG TTCTGTGTTT 
    AAATGTGTGA AGAGGCTGGA TGGATGCGTT TATGCCATAA AGCGATCAAA AAAGCCATTG 
    GCTGGCTCTG TTGATGAGCA GAATGCTTTG AGAGAAGTAT ATGCTCACGC AGTGCTTGGG 
    CAACATTCTC ATGTAGTTCG ATACTTCTCT GCCTGGGCAG AAGATGATCA TATGCTTATA 
    CAGAATGAAT ATTGTAACGG TGGCAGTTTA GCCGATGCCA TAAGTGAAAA CTACAGAAGT 
    ATGAGTTACT TTACAGAAGC AGAGTTGAAG GATCTCCTCT TACAAGTTGG CCGGGGCTTG 
    AAGTATATTC ATTCAATGTC TTTAGTTCAC ATGGATATAA AGCCTAGTAA TATTTTCATA 
    TCTCGAACTT CAATCCCAAA TGCTGCCTCT GAAGAAGGAG ATGAAGATGA CTGGGCATCC 
    AACAAAGTTA TGTTTAAAAT AGGTGACCTT GGACATGTAA CAAGGATCTC TAGTCCACAA 
    GTTGAAGAGG GTGATAGCCG TTTTCTTGCA AATGAAGTTT TACAGGAGAA CTATACTCAC 
    CTACCAAAAG CAGATATTTT TGCCCTTGCC CTCACAGTGG TATGTGCTGC TGGTGCTGAG 
    CCTCTTCCCA GAAATGGAGA CCAATGGCAT GAAATTAGAC AGGGTAGATT ACCTCGGATT 
    CCACAAGTGC TTTCCCAAGA ATTTACAGAG TTGCTAAAAG TTATGATTCA TCCAGATCCA 
    GAGAGAAGAC CTTCAGCAAT GGCACTGGTA AAGCATTCAG TATTACTGTC TGCTTCAAGG 
    AAGAGTGCAG AACAATTACG AATAGAATTG AATGCTGAAA AGTTCAAAAA TTCACTTTTG 
    CAGAAAGAAC TCAAGAAAGC CCAGATGGCA AAAGCTGCAG CTGAGGAAAG AGCACTCTTC 
    ACTGATCGGA TGGCCACCAG GTCCACCACC CAGAGTAATA GAACATCTCG ACTTATTGGA 
    AAGAAAATGA ACCGCTCTGT CAGCCTTACC ATATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    WEE1 ( XM_006051581.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006051643.1 Bubalus bubalis WEE1 G2 checkpoint kinase (WEE1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OBu50622
    Clone ID Related Accession (Same CDS sequence) XM_006051581.2
    Accession Version XM_006051581.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product wee1-like protein kinase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006051581.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGATACAG AAAAATCAGG AAAAAGGGAA TTTGATGTTC GACAGACTCC TCAAGTGAAT 
    ATTAATCCTT TTACTCCAGA TTCTCTGTTA CTGCATTCCT CAGGACAGTG TCGTAGAAGA 
    AAGAGAGCAT ATTGGAATGA TTCCTGTGGT GAAGACATGG AAGCCAGTGA TTATGAGTTT 
    GAAGATGAAA CAAGACCTGC TAAAAGAATT ACAATTACTG AAAGCAATAT GAAGTCTCGA 
    TATACAACAG AATTTCATGA GCTAGAGAAG ATTGGTTCTG GAGAATTTGG TTCTGTGTTT 
    AAATGTGTGA AGAGGCTGGA TGGATGCGTT TATGCCATAA AGCGATCAAA AAAGCCATTG 
    GCTGGCTCTG TTGATGAGCA GAATGCTTTG AGAGAAGTAT ATGCTCACGC AGTGCTTGGG 
    CAACATTCTC ATGTAGTTCG ATACTTCTCT GCCTGGGCAG AAGATGATCA TATGCTTATA 
    CAGAATGAAT ATTGTAACGG TGGCAGTTTA GCCGATGCCA TAAGTGAAAA CTACAGAAGT 
    ATGAGTTACT TTACAGAAGC AGAGTTGAAG GATCTCCTCT TACAAGTTGG CCGGGGCTTG 
    AAGTATATTC ATTCAATGTC TTTAGTTCAC ATGGATATAA AGCCTAGTAA TATTTTCATA 
    TCTCGAACTT CAATCCCAAA TGCTGCCTCT GAAGAAGGAG ATGAAGATGA CTGGGCATCC 
    AACAAAGTTA TGTTTAAAAT AGGTGACCTT GGACATGTAA CAAGGATCTC TAGTCCACAA 
    GTTGAAGAGG GTGATAGCCG TTTTCTTGCA AATGAAGTTT TACAGGAGAA CTATACTCAT 
    CTACCAAAAG CAGATATTTT TGCCCTTGCC CTCACAGTGG TATGTGCTGC TGGTGCTGAG 
    CCTCTTCCCA GAAATGGAGA CCAATGGCAT GAAATTAGAC AGGGTAGATT ACCTCGGATT 
    CCACAAGTGC TTTCCCAAGA ATTTACAGAG TTGCTAAAAG TTATGATTCA TCCAGATCCA 
    GAGAGAAGAC CTTCAGCAAT GGCACTGGTA AAGCATTCAG TATTACTGTC TGCTTCAAGG 
    AAGAGTGCAG AACAATTACG AATAGAATTG AATGCTGAAA AGTTCAAAAA TTCACTTTTG 
    CAGAAAGAAC TCAAGAAAGC CCAGATGGCA AAAGCTGCAG CTGAGGAAAG AGCACTCTTC 
    ACTGATCGGA TGGCCACCAG GTCCACCACC CAGAGTAATA GAACATCTCG ACTTATTGGA 
    AAGAAAATGA ACCGCTCTGT CAGCCTTACC ATATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006051643.1
    CDS667..1965
    Translation

    Target ORF information:

    RefSeq Version XM_006051581.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis WEE1 G2 checkpoint kinase (WEE1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006051581.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGATACAG AAAAATCAGG AAAAAGGGAA TTTGATGTTC GACAGACTCC TCAAGTGAAT 
    ATTAATCCTT TTACTCCAGA TTCTCTGTTA CTGCATTCCT CAGGACAGTG TCGTAGAAGA 
    AAGAGAGCAT ATTGGAATGA TTCCTGTGGT GAAGACATGG AAGCCAGTGA TTATGAGTTT 
    GAAGATGAAA CAAGACCTGC TAAAAGAATT ACAATTACTG AAAGCAATAT GAAGTCTCGA 
    TATACAACAG AATTTCATGA GCTAGAGAAG ATTGGTTCTG GAGAATTTGG TTCTGTGTTT 
    AAATGTGTGA AGAGGCTGGA TGGATGCGTT TATGCCATAA AGCGATCAAA AAAGCCATTG 
    GCTGGCTCTG TTGATGAGCA GAATGCTTTG AGAGAAGTAT ATGCTCACGC AGTGCTTGGG 
    CAACATTCTC ATGTAGTTCG ATACTTCTCT GCCTGGGCAG AAGATGATCA TATGCTTATA 
    CAGAATGAAT ATTGTAACGG TGGCAGTTTA GCCGATGCCA TAAGTGAAAA CTACAGAAGT 
    ATGAGTTACT TTACAGAAGC AGAGTTGAAG GATCTCCTCT TACAAGTTGG CCGGGGCTTG 
    AAGTATATTC ATTCAATGTC TTTAGTTCAC ATGGATATAA AGCCTAGTAA TATTTTCATA 
    TCTCGAACTT CAATCCCAAA TGCTGCCTCT GAAGAAGGAG ATGAAGATGA CTGGGCATCC 
    AACAAAGTTA TGTTTAAAAT AGGTGACCTT GGACATGTAA CAAGGATCTC TAGTCCACAA 
    GTTGAAGAGG GTGATAGCCG TTTTCTTGCA AATGAAGTTT TACAGGAGAA CTATACTCAT 
    CTACCAAAAG CAGATATTTT TGCCCTTGCC CTCACAGTGG TATGTGCTGC TGGTGCTGAG 
    CCTCTTCCCA GAAATGGAGA CCAATGGCAT GAAATTAGAC AGGGTAGATT ACCTCGGATT 
    CCACAAGTGC TTTCCCAAGA ATTTACAGAG TTGCTAAAAG TTATGATTCA TCCAGATCCA 
    GAGAGAAGAC CTTCAGCAAT GGCACTGGTA AAGCATTCAG TATTACTGTC TGCTTCAAGG 
    AAGAGTGCAG AACAATTACG AATAGAATTG AATGCTGAAA AGTTCAAAAA TTCACTTTTG 
    CAGAAAGAAC TCAAGAAAGC CCAGATGGCA AAAGCTGCAG CTGAGGAAAG AGCACTCTTC 
    ACTGATCGGA TGGCCACCAG GTCCACCACC CAGAGTAATA GAACATCTCG ACTTATTGGA 
    AAGAAAATGA ACCGCTCTGT CAGCCTTACC ATATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    WEE1 ( XM_006051580.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006051642.2 Bubalus bubalis WEE1 G2 checkpoint kinase (WEE1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OBu50621
    Clone ID Related Accession (Same CDS sequence) XM_006051580.2
    Accession Version XM_006051580.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product wee1-like protein kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006051580.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAGCTTCC TGAGCCGGCA GCAGCCGCCG CCGCCCCGCC GCGCCGCGGC CTCCTGCACG 
    TTGCGGCAGA AGCTGATCTT CTCGCCCTGC AGCGATTGTG AGGAGGAGGA GGAAGAAGAG 
    GAGGAAGAAG GCAGCGGCCA CAGCACCGGG GAGGACTCGG CCTTTCAGGA GCCCGACTCG 
    CCTCTGCCTC CCGCGAGGAG CCCCACAGAG CCCGGGCCCG AGCGCCGTCG CTCTCCGGAC 
    ACTATCCCCG GCAGCCCGGG GGAGCTAGAG GAGGACATGC TGCTGCGGGG CGCCTGCCCC 
    GGCGCGGATG CGGCGGGCGG CGGGGCAGAG GGAGACTCGT GGGAGGAGGA GGGCTTCGGC 
    TCCTCGTCTC CGGTCAAGTC GCCGGCCGCG CCCTACTTTT TGGGCAGCTC GTTCTCTCCA 
    GTGCGTTGCG GAGGCCCGGG GGATGCGTCG CTGCGGGGCT GCGGGGCGCG CGGGGCCGGC 
    GAGGGCGCCT GCTCGCCGCT GCCAGACTAC CCGGGTACCC CGCCCCACAA GACCTTCCGC 
    AAGCTGCGGC TCTTCGACAC GCCGCACACC CCCAAGAGTT TGCTCTCCAA AGCTCGAGGA 
    ATCGATTCCA GTTCTGTTAA ACTCCGAGGT GGCTCTCTAT TCATGGATAC AGAAAAATCA 
    GGAAAAAGGG AATTTGATGT TCGACAGACT CCTCAAGTGA ATATTAATCC TTTTACTCCA 
    GATTCTCTGT TACTGCATTC CTCAGGACAG TGTCGTAGAA GAAAGAGAGC ATATTGGAAT 
    GATTCCTGTG GTGAAGACAT GGAAGCCAGT GATTATGAGT TTGAAGATGA AACAAGACCT 
    GCTAAAAGAA TTACAATTAC TGAAAGCAAT ATGAAGTCTC GATATACAAC AGAATTTCAT 
    GAGCTAGAGA AGATTGGTTC TGGAGAATTT GGTTCTGTGT TTAAATGTGT GAAGAGGCTG 
    GATGGATGCG TTTATGCCAT AAAGCGATCA AAAAAGCCAT TGGCTGGCTC TGTTGATGAG 
    CAGAATGCTT TGAGAGAAGT ATATGCTCAC GCAGTGCTTG GGCAACATTC TCATGTAGTT 
    CGATACTTCT CTGCCTGGGC AGAAGATGAT CATATGCTTA TACAGAATGA ATATTGTAAC 
    GGTGGCAGTT TAGCCGATGC CATAAGTGAA AACTACAGAA GTATGAGTTA CTTTACAGAA 
    GCAGAGTTGA AGGATCTCCT CTTACAAGTT GGCCGGGGCT TGAAGTATAT TCATTCAATG 
    TCTTTAGTTC ACATGGATAT AAAGCCTAGT AATATTTTCA TATCTCGAAC TTCAATCCCA 
    AATGCTGCCT CTGAAGAAGG AGATGAAGAT GACTGGGCAT CCAACAAAGT TATGTTTAAA 
    ATAGGTGACC TTGGACATGT AACAAGGATC TCTAGTCCAC AAGTTGAAGA GGGTGATAGC 
    CGTTTTCTTG CAAATGAAGT TTTACAGGAG AACTATACTC ATCTACCAAA AGCAGATATT 
    TTTGCCCTTG CCCTCACAGT GGTATGTGCT GCTGGTGCTG AGCCTCTTCC CAGAAATGGA 
    GACCAATGGC ATGAAATTAG ACAGGGTAGA TTACCTCGGA TTCCACAAGT GCTTTCCCAA 
    GAATTTACAG AGTTGCTAAA AGTTATGATT CATCCAGATC CAGAGAGAAG ACCTTCAGCA 
    ATGGCACTGG TAAAGCATTC AGTATTACTG TCTGCTTCAA GGAAGAGTGC AGAACAATTA 
    CGAATAGAAT TGAATGCTGA AAAGTTCAAA AATTCACTTT TGCAGAAAGA ACTCAAGAAA 
    GCCCAGATGG CAAAAGCTGC AGCTGAGGAA AGAGCACTCT TCACTGATCG GATGGCCACC 
    AGGTCCACCA CCCAGAGTAA TAGAACATCT CGACTTATTG GAAAGAAAAT GAACCGCTCT 
    GTCAGCCTTA CCATATACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006051642.2
    CDS116..2056
    Translation

    Target ORF information:

    RefSeq Version XM_006051580.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis WEE1 G2 checkpoint kinase (WEE1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006051580.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAGCTTCC TGAGCCGGCA GCAGCCGCCG CCGCCCCGCC GCGCCGCGGC CTCCTGCACG 
    TTGCGGCAGA AGCTGATCTT CTCGCCCTGC AGCGATTGTG AGGAGGAGGA GGAAGAAGAG 
    GAGGAAGAAG GCAGCGGCCA CAGCACCGGG GAGGACTCGG CCTTTCAGGA GCCCGACTCG 
    CCTCTGCCTC CCGCGAGGAG CCCCACAGAG CCCGGGCCCG AGCGCCGTCG CTCTCCGGAC 
    ACTATCCCCG GCAGCCCGGG GGAGCTAGAG GAGGACATGC TGCTGCGGGG CGCCTGCCCC 
    GGCGCGGATG CGGCGGGCGG CGGGGCAGAG GGAGACTCGT GGGAGGAGGA GGGCTTCGGC 
    TCCTCGTCTC CGGTCAAGTC GCCGGCCGCG CCCTACTTTT TGGGCAGCTC GTTCTCTCCA 
    GTGCGTTGCG GAGGCCCGGG GGATGCGTCG CTGCGGGGCT GCGGGGCGCG CGGGGCCGGC 
    GAGGGCGCCT GCTCGCCGCT GCCAGACTAC CCGGGTACCC CGCCCCACAA GACCTTCCGC 
    AAGCTGCGGC TCTTCGACAC GCCGCACACC CCCAAGAGTT TGCTCTCCAA AGCTCGAGGA 
    ATCGATTCCA GTTCTGTTAA ACTCCGAGGT GGCTCTCTAT TCATGGATAC AGAAAAATCA 
    GGAAAAAGGG AATTTGATGT TCGACAGACT CCTCAAGTGA ATATTAATCC TTTTACTCCA 
    GATTCTCTGT TACTGCATTC CTCAGGACAG TGTCGTAGAA GAAAGAGAGC ATATTGGAAT 
    GATTCCTGTG GTGAAGACAT GGAAGCCAGT GATTATGAGT TTGAAGATGA AACAAGACCT 
    GCTAAAAGAA TTACAATTAC TGAAAGCAAT ATGAAGTCTC GATATACAAC AGAATTTCAT 
    GAGCTAGAGA AGATTGGTTC TGGAGAATTT GGTTCTGTGT TTAAATGTGT GAAGAGGCTG 
    GATGGATGCG TTTATGCCAT AAAGCGATCA AAAAAGCCAT TGGCTGGCTC TGTTGATGAG 
    CAGAATGCTT TGAGAGAAGT ATATGCTCAC GCAGTGCTTG GGCAACATTC TCATGTAGTT 
    CGATACTTCT CTGCCTGGGC AGAAGATGAT CATATGCTTA TACAGAATGA ATATTGTAAC 
    GGTGGCAGTT TAGCCGATGC CATAAGTGAA AACTACAGAA GTATGAGTTA CTTTACAGAA 
    GCAGAGTTGA AGGATCTCCT CTTACAAGTT GGCCGGGGCT TGAAGTATAT TCATTCAATG 
    TCTTTAGTTC ACATGGATAT AAAGCCTAGT AATATTTTCA TATCTCGAAC TTCAATCCCA 
    AATGCTGCCT CTGAAGAAGG AGATGAAGAT GACTGGGCAT CCAACAAAGT TATGTTTAAA 
    ATAGGTGACC TTGGACATGT AACAAGGATC TCTAGTCCAC AAGTTGAAGA GGGTGATAGC 
    CGTTTTCTTG CAAATGAAGT TTTACAGGAG AACTATACTC ATCTACCAAA AGCAGATATT 
    TTTGCCCTTG CCCTCACAGT GGTATGTGCT GCTGGTGCTG AGCCTCTTCC CAGAAATGGA 
    GACCAATGGC ATGAAATTAG ACAGGGTAGA TTACCTCGGA TTCCACAAGT GCTTTCCCAA 
    GAATTTACAG AGTTGCTAAA AGTTATGATT CATCCAGATC CAGAGAGAAG ACCTTCAGCA 
    ATGGCACTGG TAAAGCATTC AGTATTACTG TCTGCTTCAA GGAAGAGTGC AGAACAATTA 
    CGAATAGAAT TGAATGCTGA AAAGTTCAAA AATTCACTTT TGCAGAAAGA ACTCAAGAAA 
    GCCCAGATGG CAAAAGCTGC AGCTGAGGAA AGAGCACTCT TCACTGATCG GATGGCCACC 
    AGGTCCACCA CCCAGAGTAA TAGAACATCT CGACTTATTG GAAAGAAAAT GAACCGCTCT 
    GTCAGCCTTA CCATATACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    WEE1 ( XM_006051580.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006051642.1 Bubalus bubalis WEE1 G2 checkpoint kinase (WEE1), transcript variant X1, mRNA.
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    CloneID OBu09464
    Clone ID Related Accession (Same CDS sequence) XM_006051580.1
    Accession Version XM_006051580.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product wee1-like protein kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784387.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAGCTTCC TGAGCCGGCA GCAGCCGCCG CCGCCCCGCC GCGCCGCGGC CTCCTGCACG 
    TTGCGGCAGA AGCTGATCTT CTCGCCCTGC AGCGATTGTG AGGAGGAGGA GGAAGAAGAG 
    GAGGAAGAAG GCAGCGGCCA CAGCACCGGG GAGGACTCGG CCTTTCAGGA GCCCGACTCG 
    CCTCTGCCTC CCGCGAGGAG CCCCACAGAG CCCGGGCCCG AGCGCCGTCG CTCTCCGGAC 
    ACTATCCCCG GCAGCCCGGG GGAGCTAGAG GAGGACATGC TGCTGCGGGG CGCCTGCCCC 
    GGCGCGGATG CGGCGGGCGG CGGGGCAGAG GGAGACTCGT GGGAGGAGGA GGGCTTCGGC 
    TCCTCGTCTC CGGTCAAGTC GCCGGCCGCG CCCTACTTTT TGGGCAGCTC GTTCTCTCCA 
    GTGCGTTGCG GAGGCCCGGG GGATGCGTCG CTGCGGGGCT GCGGGGCGCG CGGGGTCGGC 
    GAGGGCGCCT GCTCGCCGCT GCCAGACTAC CCGGGTACCC CGCCCCACAA GACCTTCCGC 
    AAGCTGCGGC TCTTCGACAC GCCGCACACC CCCAAGAGTT TGCTCTCCAA AGCTCGAGGA 
    ATCGATTCCA GTTCTGTTAA ACTCCGAGGT GGCTCTCTAT TCATGGATAC AGAAAAATCA 
    GGAAAAAGGG AATTTGATGT TCGACAGACT CCTCAAGTGA ATATTAATCC TTTTACTCCA 
    GATTCTCTGT TACTGCATTC CTCAGGACAG TGTCGTAGAA GAAAGAGAGC ATATTGGAAT 
    GATTCCTGTG GTGAAGACAT GGAAGCCAGT GATTATGAGT TTGAAGATGA AACAAGACCT 
    GCTAAAAGAA TTACAATTAC TGAAAGCAAT ATGAAGTCTC GATATACAAC AGAATTTCAT 
    GAGCTAGAGA AGATTGGTTC TGGAGAATTT GGTTCTGTGT TTAAATGTGT GAAGAGGCTG 
    GATGGATGCG TTTATGCCAT AAAGCGATCA AAAAAGCCAT TGGCTGGCTC TGTTGATGAG 
    CAGAATGCTT TGAGAGAAGT ATATGCTCAC GCAGTGCTTG GGCAACATTC TCATGTAGTT 
    CGATACTTCT CTGCCTGGGC AGAAGATGAT CATATGCTTA TACAGAATGA ATATTGTAAC 
    GGTGGCAGTT TAGCCGATGC CATAAGTGAA AACTACAGAA GTATGAGTTA CTTTACAGAA 
    GCAGAGTTGA AGGATCTCCT CTTACAAGTT GGCCGGGGCT TGAAGTATAT TCATTCAATG 
    TCTTTAGTTC ACATGGATAT AAAGCCTAGT AATATTTTCA TATCTCGAAC TTCAATCCCA 
    AATGCTGCCT CTGAAGAAGG AGATGAAGAT GACTGGGCAT CCAACAAAGT TATGTTTAAA 
    ATAGGTGACC TTGGACATGT AACAAGGATC TCTAGTCCAC AAGTTGAAGA GGGTGATAGC 
    CGTTTTCTTG CAAATGAAGT TTTACAGGAG AACTATACTC ACCTACCAAA AGCAGATATT 
    TTTGCCCTTG CCCTCACAGT GGTATGTGCT GCTGGTGCTG AGCCTCTTCC CAGAAATGGA 
    GACCAATGGC ATGAAATTAG ACAGGGTAGA TTACCTCGGA TTCCACAAGT GCTTTCCCAA 
    GAATTTACAG AGTTGCTAAA AGTTATGATT CATCCAGATC CAGAGAGAAG ACCTTCAGCA 
    ATGGCACTGG TAAAGCATTC AGTATTACTG TCTGCTTCAA GGAAGAGTGC AGAACAATTA 
    CGAATAGAAT TGAATGCTGA AAAGTTCAAA AATTCACTTT TGCAGAAAGA ACTCAAGAAA 
    GCCCAGATGG CAAAAGCTGC AGCTGAGGAA AGAGCACTCT TCACTGATCG GATGGCCACC 
    AGGTCCACCA CCCAGAGTAA TAGAACATCT CGACTTATTG GAAAGAAAAT GAACCGCTCT 
    GTCAGCCTTA CCATATACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006051642.1
    CDS67..2007
    Translation

    Target ORF information:

    RefSeq Version XM_006051580.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis WEE1 G2 checkpoint kinase (WEE1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006051580.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAGCTTCC TGAGCCGGCA GCAGCCGCCG CCGCCCCGCC GCGCCGCGGC CTCCTGCACG 
    TTGCGGCAGA AGCTGATCTT CTCGCCCTGC AGCGATTGTG AGGAGGAGGA GGAAGAAGAG 
    GAGGAAGAAG GCAGCGGCCA CAGCACCGGG GAGGACTCGG CCTTTCAGGA GCCCGACTCG 
    CCTCTGCCTC CCGCGAGGAG CCCCACAGAG CCCGGGCCCG AGCGCCGTCG CTCTCCGGAC 
    ACTATCCCCG GCAGCCCGGG GGAGCTAGAG GAGGACATGC TGCTGCGGGG CGCCTGCCCC 
    GGCGCGGATG CGGCGGGCGG CGGGGCAGAG GGAGACTCGT GGGAGGAGGA GGGCTTCGGC 
    TCCTCGTCTC CGGTCAAGTC GCCGGCCGCG CCCTACTTTT TGGGCAGCTC GTTCTCTCCA 
    GTGCGTTGCG GAGGCCCGGG GGATGCGTCG CTGCGGGGCT GCGGGGCGCG CGGGGTCGGC 
    GAGGGCGCCT GCTCGCCGCT GCCAGACTAC CCGGGTACCC CGCCCCACAA GACCTTCCGC 
    AAGCTGCGGC TCTTCGACAC GCCGCACACC CCCAAGAGTT TGCTCTCCAA AGCTCGAGGA 
    ATCGATTCCA GTTCTGTTAA ACTCCGAGGT GGCTCTCTAT TCATGGATAC AGAAAAATCA 
    GGAAAAAGGG AATTTGATGT TCGACAGACT CCTCAAGTGA ATATTAATCC TTTTACTCCA 
    GATTCTCTGT TACTGCATTC CTCAGGACAG TGTCGTAGAA GAAAGAGAGC ATATTGGAAT 
    GATTCCTGTG GTGAAGACAT GGAAGCCAGT GATTATGAGT TTGAAGATGA AACAAGACCT 
    GCTAAAAGAA TTACAATTAC TGAAAGCAAT ATGAAGTCTC GATATACAAC AGAATTTCAT 
    GAGCTAGAGA AGATTGGTTC TGGAGAATTT GGTTCTGTGT TTAAATGTGT GAAGAGGCTG 
    GATGGATGCG TTTATGCCAT AAAGCGATCA AAAAAGCCAT TGGCTGGCTC TGTTGATGAG 
    CAGAATGCTT TGAGAGAAGT ATATGCTCAC GCAGTGCTTG GGCAACATTC TCATGTAGTT 
    CGATACTTCT CTGCCTGGGC AGAAGATGAT CATATGCTTA TACAGAATGA ATATTGTAAC 
    GGTGGCAGTT TAGCCGATGC CATAAGTGAA AACTACAGAA GTATGAGTTA CTTTACAGAA 
    GCAGAGTTGA AGGATCTCCT CTTACAAGTT GGCCGGGGCT TGAAGTATAT TCATTCAATG 
    TCTTTAGTTC ACATGGATAT AAAGCCTAGT AATATTTTCA TATCTCGAAC TTCAATCCCA 
    AATGCTGCCT CTGAAGAAGG AGATGAAGAT GACTGGGCAT CCAACAAAGT TATGTTTAAA 
    ATAGGTGACC TTGGACATGT AACAAGGATC TCTAGTCCAC AAGTTGAAGA GGGTGATAGC 
    CGTTTTCTTG CAAATGAAGT TTTACAGGAG AACTATACTC ACCTACCAAA AGCAGATATT 
    TTTGCCCTTG CCCTCACAGT GGTATGTGCT GCTGGTGCTG AGCCTCTTCC CAGAAATGGA 
    GACCAATGGC ATGAAATTAG ACAGGGTAGA TTACCTCGGA TTCCACAAGT GCTTTCCCAA 
    GAATTTACAG AGTTGCTAAA AGTTATGATT CATCCAGATC CAGAGAGAAG ACCTTCAGCA 
    ATGGCACTGG TAAAGCATTC AGTATTACTG TCTGCTTCAA GGAAGAGTGC AGAACAATTA 
    CGAATAGAAT TGAATGCTGA AAAGTTCAAA AATTCACTTT TGCAGAAAGA ACTCAAGAAA 
    GCCCAGATGG CAAAAGCTGC AGCTGAGGAA AGAGCACTCT TCACTGATCG GATGGCCACC 
    AGGTCCACCA CCCAGAGTAA TAGAACATCT CGACTTATTG GAAAGAAAAT GAACCGCTCT 
    GTCAGCCTTA CCATATACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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