kalrn cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following kalrn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kalrn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa24909 NM_001016029.3
Latest version!
Xenopus tropicalis kalirin RhoGEF kinase (kalrn), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OXa66022 XM_031892641.1
Latest version!
Xenopus tropicalis kalirin RhoGEF kinase (kalrn), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa66023 XM_031892642.1
Latest version!
Xenopus tropicalis kalirin RhoGEF kinase (kalrn), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa66024 XM_031892643.1
Latest version!
Xenopus tropicalis kalirin RhoGEF kinase (kalrn), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa66025 XM_031892644.1
Latest version!
Xenopus tropicalis kalirin RhoGEF kinase (kalrn), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa66026 XM_031892645.1
Latest version!
Xenopus tropicalis kalirin RhoGEF kinase (kalrn), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa27528 XM_012970399.2
Latest version!
Xenopus tropicalis kalirin, RhoGEF kinase (kalrn), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa66017 XM_031892635.1
Latest version!
Xenopus tropicalis kalirin RhoGEF kinase (kalrn), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa66018 XM_031892636.1
Latest version!
Xenopus tropicalis kalirin RhoGEF kinase (kalrn), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa66019 XM_031892637.1
Latest version!
Xenopus tropicalis kalirin RhoGEF kinase (kalrn), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa66020 XM_031892639.1
Latest version!
Xenopus tropicalis kalirin RhoGEF kinase (kalrn), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa66021 XM_031892640.1
Latest version!
Xenopus tropicalis kalirin RhoGEF kinase (kalrn), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
View Old Accession Versions >>
OXa24909 NM_001016029.2 Xenopus tropicalis kalirin RhoGEF kinase (kalrn), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa24909
    Clone ID Related Accession (Same CDS sequence) NM_001016029.2 , NM_001016029.3
    Accession Version NM_001016029.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1989bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-16
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kalirin RhoGEF kinase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR848132.2. On Oct 14, 2005 this sequence version replaced NM_001016029.1. ##Evidence-Data-START## Transcript exon combination :: CR848132.2, BC170882.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1025688, SAMEA1696415 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAAGGGTG GCACTGCCAA GTACCCCTCG GGCTCCTGCC TGGGGTGGCT CTGTACCCGC 
    TGCTGCTGCT GCTTCTTTGC AACAGATTCA TTTTCTGTCT CCGCGGCGGA AAATGGGGGG
    AAGTCCGAGT CGGTGGCCAA TCTGCAGCCC CAGGCGTCCC TGAATTCTAT CCACAGCTCC
    CCGGGCCCCA AACGCTCCAC CAACACCCTA AAAAAGTGGC TGACGAGCCC TGTGAGGCGA
    CTGAACAGCG GGAAGGGCGA GAGCAACATC AAGAAGCAAA AGAAAAGAGA CGGGCGCAAC
    CTCGGCTCCC CCAGAGCTGG GGACGAGACC ACCCCACAGG GAGACAGCGC CGATGAGAAG
    GGCAAGAAGG GGTGGGACGA AGAGGCTGAC GACGAATCCC ACACTCCGCT TCCTCCGCCA
    ATGAAGATCT TTGATAATGA CCCCACTCCG GACGACAAGA CCATTGACGG AGGTTCTTAT
    CGGGGCGCAC AGAACATAAA TGAAGGATTT GTGAATGAGG GTTTGGCCCC CTCGCCAGCT
    CCCAGGAACC ACGAAGAGGA GCAAAAAGCC AAAGCACTCA GGGGCAGGAT GTTTGTACTG
    AATGAGCTGG TACAGACGGA GAAGGACTAT GTCCGGGATC TTGGGTTTAT GGTAGAGACG
    TACATCCCCA AGATGGAGGA GAGGGGGACC CCGGAAGATA TGAACGGAAA AGACAAGATC
    GTATTCGGCA ACATCCACCA GATCTACGAT TGGCACAAAG ACTTTTACTT GGCAGAACTA
    GAGAAATGCC TATTGGAGCC GGAGAGGTTG GCGCAGTTAT TTATAAAACA TGAGAGGCGG
    CTGCACATGT ATGTGGTTTA TTGCCAAAAC AAACCGCGCT CGGAGTTTGT CGTTGCCGAA
    TATGACGCCT ACTTTGAGGA TCTCATGCAG GAGGTGAATC CCAGATTTAC TGTCAGTGAT
    TTTCTCATCA AACCGATCCA AAGGATAACT AAGTACCAAC TGCTGCTAAA GGACTTCCTT
    AAGTACAGTC AGAAGGCGGG TTTGGAGTGT TCAGAAATCG AGAAAGCCGT GGAACTAATG
    TGCCTTGTCC CGAAACGCTG CAACGACATG ATGAACCTGG GGCGCCTGCA GGGATTCGAG
    GGGAAGCTGA CAGCCCAGGG GAAGCTGTTG CAGCAGGACA CCTTCTATGT GATCGAGCAG
    GAATCGGGGG TCCAGTCTCG GACGAAGGAA AGGCGGGTTT TCCTGTTCGA GCAGATTGTT
    ATTTTCAGCG AGCTCCTGCG GAAAGGTTCC TCCACGCCGG GGTACATGTT CAAAAGGGGC
    ATAAAGATGA ATTATCTCAT CTTAGAAGAG AACATTGACA ACGACCCGTG CAAATTCGCC
    ATTCGGAGCC GCGAGACGTC GGAGCGGGTG ATCCTGCAAG CGGCCAACGC CGACATTAAA
    CAGGCCTGGG TGCAGGACAT CGGGCAAGTG CTGGAGACCC AGAGGGACTT CCTAAACGCC
    CTGCAATCGC CAATAGAATA CCAGCGGAAG GAGAGCAACG CAGCCATGAA CAAGCCTCAG
    ATTGGCCGCG GGCCGTCCCC CACCGTTAGG CCTCTCTCCT CTACGCCCGT AGGGTGTGAA
    AAAGATGTCA CCATCCCAGT GCGGAACCAG TCGCTACCAG CGCGGAAAAT ACCTACCTCT
    AACGGCAGCG CCGGGTATGA AATGTACCCA CGGGCGGAGA AATGTGACCC AAACCAGAGC
    GAGCTGAGCG GCTGCAATGG GACCAACTCC ATGGTGGTTG TACAGGATTA CTATGCACTA
    AAGGAGAATG AGATCTGCGT GTACCACGGG GAGTTGGTAC AGATTCTGGC CATCAATCAG
    CAGAACATGT TCCTGGTATT CCGGCCGTCC AATGACCATT CCCCGGCAGC GGAAGGCTGG
    ATCCCGGGAT CAGTTTTGGG CCACATTTCT AAACCTTCCG CAGAAAATAG CGACAGATCC
    ATCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001016029.1
    CDS36..2024
    Translation

    Target ORF information:

    RefSeq Version NM_001016029.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kalirin RhoGEF kinase (kalrn), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001016029.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAAGGGTG GCACTGCCAA GTACCCCTCG GGCTCCTGCC TGGGGTGGCT CTGTACCCGC 
    TGCTGCTGCT GCTTCTTTGC AACAGATTCA TTTTCTGTCT CCGCGGCGGA AAATGGGGGG
    AAGTCCGAGT CGGTGGCCAA TCTGCAGCCC CAGGCGTCCC TGAATTCTAT CCACAGCTCC
    CCGGGCCCCA AACGCTCCAC CAACACCCTA AAAAAGTGGC TGACGAGCCC TGTGAGGCGA
    CTGAACAGCG GGAAGGGCGA GAGCAACATC AAGAAGCAAA AGAAAAGAGA CGGGCGCAAC
    CTCGGCTCCC CCAGAGCTGG GGACGAGACC ACCCCACAGG GAGACAGCGC CGATGAGAAG
    GGCAAGAAGG GGTGGGACGA AGAGGCTGAC GACGAATCCC ACACTCCGCT TCCTCCGCCA
    ATGAAGATCT TTGATAATGA CCCCACTCCG GACGACAAGA CCATTGACGG AGGTTCTTAT
    CGGGGCGCAC AGAACATAAA TGAAGGATTT GTGAATGAGG GTTTGGCCCC CTCGCCAGCT
    CCCAGGAACC ACGAAGAGGA GCAAAAAGCC AAAGCACTCA GGGGCAGGAT GTTTGTACTG
    AATGAGCTGG TACAGACGGA GAAGGACTAT GTCCGGGATC TTGGGTTTAT GGTAGAGACG
    TACATCCCCA AGATGGAGGA GAGGGGGACC CCGGAAGATA TGAACGGAAA AGACAAGATC
    GTATTCGGCA ACATCCACCA GATCTACGAT TGGCACAAAG ACTTTTACTT GGCAGAACTA
    GAGAAATGCC TATTGGAGCC GGAGAGGTTG GCGCAGTTAT TTATAAAACA TGAGAGGCGG
    CTGCACATGT ATGTGGTTTA TTGCCAAAAC AAACCGCGCT CGGAGTTTGT CGTTGCCGAA
    TATGACGCCT ACTTTGAGGA TCTCATGCAG GAGGTGAATC CCAGATTTAC TGTCAGTGAT
    TTTCTCATCA AACCGATCCA AAGGATAACT AAGTACCAAC TGCTGCTAAA GGACTTCCTT
    AAGTACAGTC AGAAGGCGGG TTTGGAGTGT TCAGAAATCG AGAAAGCCGT GGAACTAATG
    TGCCTTGTCC CGAAACGCTG CAACGACATG ATGAACCTGG GGCGCCTGCA GGGATTCGAG
    GGGAAGCTGA CAGCCCAGGG GAAGCTGTTG CAGCAGGACA CCTTCTATGT GATCGAGCAG
    GAATCGGGGG TCCAGTCTCG GACGAAGGAA AGGCGGGTTT TCCTGTTCGA GCAGATTGTT
    ATTTTCAGCG AGCTCCTGCG GAAAGGTTCC TCCACGCCGG GGTACATGTT CAAAAGGGGC
    ATAAAGATGA ATTATCTCAT CTTAGAAGAG AACATTGACA ACGACCCGTG CAAATTCGCC
    ATTCGGAGCC GCGAGACGTC GGAGCGGGTG ATCCTGCAAG CGGCCAACGC CGACATTAAA
    CAGGCCTGGG TGCAGGACAT CGGGCAAGTG CTGGAGACCC AGAGGGACTT CCTAAACGCC
    CTGCAATCGC CAATAGAATA CCAGCGGAAG GAGAGCAACG CAGCCATGAA CAAGCCTCAG
    ATTGGCCGCG GGCCGTCCCC CACCGTTAGG CCTCTCTCCT CTACGCCCGT AGGGTGTGAA
    AAAGATGTCA CCATCCCAGT GCGGAACCAG TCGCTACCAG CGCGGAAAAT ACCTACCTCT
    AACGGCAGCG CCGGGTATGA AATGTACCCA CGGGCGGAGA AATGTGACCC AAACCAGAGC
    GAGCTGAGCG GCTGCAATGG GACCAACTCC ATGGTGGTTG TACAGGATTA CTATGCACTA
    AAGGAGAATG AGATCTGCGT GTACCACGGG GAGTTGGTAC AGATTCTGGC CATCAATCAG
    CAGAACATGT TCCTGGTATT CCGGCCGTCC AATGACCATT CCCCGGCAGC GGAAGGCTGG
    ATCCCGGGAT CAGTTTTGGG CCACATTTCT AAACCTTCCG CAGAAAATAG CGACAGATCC
    ATCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa24909
    Clone ID Related Accession (Same CDS sequence) NM_001016029.2 , NM_001016029.3
    Accession Version NM_001016029.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1989bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kalirin
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03043336.1, CR848132.2 and AAMC03043340.1. On Oct 16, 2019 this sequence version replaced NM_001016029.2. ##Evidence-Data-START## Transcript exon combination :: CR848132.2, BC171263.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1025688, SAMEA1696415 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAAGGGTG GCACTGCCAA GTACCCCTCG GGCTCCTGCC TGGGGTGGCT CTGTACCCGC 
    TGCTGCTGCT GCTTCTTTGC AACAGATTCA TTTTCTGTCT CCGCGGCGGA AAATGGGGGG
    AAGTCCGAGT CGGTGGCCAA TCTGCAGCCC CAGGCGTCCC TGAATTCTAT CCACAGCTCC
    CCGGGCCCCA AACGCTCCAC CAACACCCTA AAAAAGTGGC TGACGAGCCC TGTGAGGCGA
    CTGAACAGCG GGAAGGGCGA GAGCAACATC AAGAAGCAAA AGAAAAGAGA CGGGCGCAAC
    CTCGGCTCCC CCAGAGCTGG GGACGAGACC ACCCCACAGG GAGACAGCGC CGATGAGAAG
    GGCAAGAAGG GGTGGGACGA AGAGGCTGAC GACGAATCCC ACACTCCGCT TCCTCCGCCA
    ATGAAGATCT TTGATAATGA CCCCACTCCG GACGACAAGA CCATTGACGG AGGTTCTTAT
    CGGGGCGCAC AGAACATAAA TGAAGGATTT GTGAATGAGG GTTTGGCCCC CTCGCCAGCT
    CCCAGGAACC ACGAAGAGGA GCAAAAAGCC AAAGCACTCA GGGGCAGGAT GTTTGTACTG
    AATGAGCTGG TACAGACGGA GAAGGACTAT GTCCGGGATC TTGGGTTTAT GGTAGAGACG
    TACATCCCCA AGATGGAGGA GAGGGGGACC CCGGAAGATA TGAACGGAAA AGACAAGATC
    GTATTCGGCA ACATCCACCA GATCTACGAT TGGCACAAAG ACTTTTACTT GGCAGAACTA
    GAGAAATGCC TATTGGAGCC GGAGAGGTTG GCGCAGTTAT TTATAAAACA TGAGAGGCGG
    CTGCACATGT ATGTGGTTTA TTGCCAAAAC AAACCGCGCT CGGAGTTTGT CGTTGCCGAA
    TATGACGCCT ACTTTGAGGA TCTCATGCAG GAGGTGAATC CCAGATTTAC TGTCAGTGAT
    TTTCTCATCA AACCGATCCA AAGGATAACT AAGTACCAAC TGCTGCTAAA GGACTTCCTT
    AAGTACAGTC AGAAGGCGGG TTTGGAGTGT TCAGAAATCG AGAAAGCCGT GGAACTAATG
    TGCCTTGTCC CGAAACGCTG CAACGACATG ATGAACCTGG GGCGCCTGCA GGGATTCGAG
    GGGAAGCTGA CAGCCCAGGG GAAGCTGTTG CAGCAGGACA CCTTCTATGT GATCGAGCAG
    GAATCGGGGG TCCAGTCTCG GACGAAGGAA AGGCGGGTTT TCCTGTTCGA GCAGATTGTT
    ATTTTCAGCG AGCTCCTGCG GAAAGGTTCC TCCACGCCGG GGTACATGTT CAAAAGGGGC
    ATAAAGATGA ATTATCTCAT CTTAGAAGAG AACATTGACA ACGACCCGTG CAAATTCGCC
    ATTCGGAGCC GCGAGACGTC GGAGCGGGTG ATCCTGCAAG CGGCCAACGC CGACATTAAA
    CAGGCCTGGG TGCAGGACAT CGGGCAAGTG CTGGAGACCC AGAGGGACTT CCTAAACGCC
    CTGCAATCGC CAATAGAATA CCAGCGGAAG GAGAGCAACG CAGCCATGAA CAAGCCTCAG
    ATTGGCCGCG GGCCGTCCCC CACCGTTAGG CCTCTCTCCT CTACGCCCGT AGGGTGTGAA
    AAAGATGTCA CCATCCCAGT GCGGAACCAG TCGCTACCAG CGCGGAAAAT ACCTACCTCT
    AACGGCAGCG CCGGGTATGA AATGTACCCA CGGGCGGAGA AATGTGACCC AAACCAGAGC
    GAGCTGAGCG GCTGCAATGG GACCAACTCC ATGGTGGTTG TACAGGATTA CTATGCACTA
    AAGGAGAATG AGATCTGCGT GTACCACGGG GAGTTGGTAC AGATTCTGGC CATCAATCAG
    CAGAACATGT TCCTGGTATT CCGGCCGTCC AATGACCATT CCCCGGCAGC GGAAGGCTGG
    ATCCCGGGAT CAGTTTTGGG CCACATTTCT AAACCTTCCG CAGAAAATAG CGACAGATCC
    ATCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001016029.1
    CDS82..2070
    Translation

    Target ORF information:

    RefSeq Version NM_001016029.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kalirin RhoGEF kinase (kalrn), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001016029.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAAGGGTG GCACTGCCAA GTACCCCTCG GGCTCCTGCC TGGGGTGGCT CTGTACCCGC 
    TGCTGCTGCT GCTTCTTTGC AACAGATTCA TTTTCTGTCT CCGCGGCGGA AAATGGGGGG
    AAGTCCGAGT CGGTGGCCAA TCTGCAGCCC CAGGCGTCCC TGAATTCTAT CCACAGCTCC
    CCGGGCCCCA AACGCTCCAC CAACACCCTA AAAAAGTGGC TGACGAGCCC TGTGAGGCGA
    CTGAACAGCG GGAAGGGCGA GAGCAACATC AAGAAGCAAA AGAAAAGAGA CGGGCGCAAC
    CTCGGCTCCC CCAGAGCTGG GGACGAGACC ACCCCACAGG GAGACAGCGC CGATGAGAAG
    GGCAAGAAGG GGTGGGACGA AGAGGCTGAC GACGAATCCC ACACTCCGCT TCCTCCGCCA
    ATGAAGATCT TTGATAATGA CCCCACTCCG GACGACAAGA CCATTGACGG AGGTTCTTAT
    CGGGGCGCAC AGAACATAAA TGAAGGATTT GTGAATGAGG GTTTGGCCCC CTCGCCAGCT
    CCCAGGAACC ACGAAGAGGA GCAAAAAGCC AAAGCACTCA GGGGCAGGAT GTTTGTACTG
    AATGAGCTGG TACAGACGGA GAAGGACTAT GTCCGGGATC TTGGGTTTAT GGTAGAGACG
    TACATCCCCA AGATGGAGGA GAGGGGGACC CCGGAAGATA TGAACGGAAA AGACAAGATC
    GTATTCGGCA ACATCCACCA GATCTACGAT TGGCACAAAG ACTTTTACTT GGCAGAACTA
    GAGAAATGCC TATTGGAGCC GGAGAGGTTG GCGCAGTTAT TTATAAAACA TGAGAGGCGG
    CTGCACATGT ATGTGGTTTA TTGCCAAAAC AAACCGCGCT CGGAGTTTGT CGTTGCCGAA
    TATGACGCCT ACTTTGAGGA TCTCATGCAG GAGGTGAATC CCAGATTTAC TGTCAGTGAT
    TTTCTCATCA AACCGATCCA AAGGATAACT AAGTACCAAC TGCTGCTAAA GGACTTCCTT
    AAGTACAGTC AGAAGGCGGG TTTGGAGTGT TCAGAAATCG AGAAAGCCGT GGAACTAATG
    TGCCTTGTCC CGAAACGCTG CAACGACATG ATGAACCTGG GGCGCCTGCA GGGATTCGAG
    GGGAAGCTGA CAGCCCAGGG GAAGCTGTTG CAGCAGGACA CCTTCTATGT GATCGAGCAG
    GAATCGGGGG TCCAGTCTCG GACGAAGGAA AGGCGGGTTT TCCTGTTCGA GCAGATTGTT
    ATTTTCAGCG AGCTCCTGCG GAAAGGTTCC TCCACGCCGG GGTACATGTT CAAAAGGGGC
    ATAAAGATGA ATTATCTCAT CTTAGAAGAG AACATTGACA ACGACCCGTG CAAATTCGCC
    ATTCGGAGCC GCGAGACGTC GGAGCGGGTG ATCCTGCAAG CGGCCAACGC CGACATTAAA
    CAGGCCTGGG TGCAGGACAT CGGGCAAGTG CTGGAGACCC AGAGGGACTT CCTAAACGCC
    CTGCAATCGC CAATAGAATA CCAGCGGAAG GAGAGCAACG CAGCCATGAA CAAGCCTCAG
    ATTGGCCGCG GGCCGTCCCC CACCGTTAGG CCTCTCTCCT CTACGCCCGT AGGGTGTGAA
    AAAGATGTCA CCATCCCAGT GCGGAACCAG TCGCTACCAG CGCGGAAAAT ACCTACCTCT
    AACGGCAGCG CCGGGTATGA AATGTACCCA CGGGCGGAGA AATGTGACCC AAACCAGAGC
    GAGCTGAGCG GCTGCAATGG GACCAACTCC ATGGTGGTTG TACAGGATTA CTATGCACTA
    AAGGAGAATG AGATCTGCGT GTACCACGGG GAGTTGGTAC AGATTCTGGC CATCAATCAG
    CAGAACATGT TCCTGGTATT CCGGCCGTCC AATGACCATT CCCCGGCAGC GGAAGGCTGG
    ATCCCGGGAT CAGTTTTGGG CCACATTTCT AAACCTTCCG CAGAAAATAG CGACAGATCC
    ATCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa66022
    Clone ID Related Accession (Same CDS sequence) XM_031892641.1
    Accession Version XM_031892641.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kalirin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGGCGAGT CGATGCTGAA TGCCAGTCTG GTTAATGCCA GTTCACTGTC GGAAGCTGAG 
    CAGTTGCAGC GAGAACACGA GCAGTTTCAG ATGGCTATTG AGTCCCTTTT CCACGCCAGC
    TCTCTGCAGA AGACTCACCA AAGTGCCCTG CAGGTCCAGC AGAAAGCAGA GGCGCTTCTA
    CAGGCCGGGC ACTACGACGC CGACGCCATC CGGGAATGCG CCGAGAAGGT GGCGATGCAC
    TGGCAGCAAC TCATGCTCAA GATGGAGGAC AGACTCAAGC TAGTGAATGC CTCTGTGGCC
    TTCTATAAAA CCTCCGAGCA GGTGTGCAGC GTTCTGGAGA GCCTGGAGCA GGAGTACCGC
    AGGGATGAGG ACTGGTGTCG GGGCCACGAT AAGCTGGGAC CCTCCACTGA GTCGGACCAC
    GTGATGCCGC TGATCAGTAA ACACCTGGAG CAGAAGGAGG CTTTCCTTAA GGCTTGTACG
    TTAGCGCGGA GGAACGCGGA AGTCTTTCTG AAGTACATTC ACCGTAACAA CGTGAGCATG
    CCCGGCGTGG CCCCCCACAC GCGCGTCCCC GAGCAGCAAG TTAAAGCCAT ACTGAGCGAG
    CTCCTGCAAA GGGAGAACCG GGTGCTGCAC TTTTGGACCC TGAAGAAGCG GCGGTTGGAC
    CAGTGCCAGC AGTACATTGT GTTTGAGAGG AGCGCCAAGC AGGCTCTGGA CTGGATTCAG
    GAAGCCGGGG AGCATTATCT CTCCACTCGC ATCTCCCCTG GGGAATGTAT TGAGGAGACT
    CAGGAGCTGC TGAAGGAATA CGAGGAATTC CGGATACCTG CCAAGCAAAC GAAGGAGAAG
    GTGCGGCTGC TGATCCAGCT GGCAGACAGC TTTGTGGAGA AGGGCCACGT TCACGCCACC
    GAGCTGAAGA AATGGGTCAC TACTGTCAAC AAACACTACC GGGAATTCGC TCTGCGTATG
    GGCAAATACC ATTGCGGCCT GCAGCGAGCC CTGGGCATCT GCACTGAGGA CACAAAGGAC
    CTGGAGTTGG ACATTGTCCC TGCGATTCTG ACTGACCCGG AGGTGAAGCT GCGCGACGCC
    GGCCATGAAC TCAACGAGGA GAAGAGAAAA TCCGCCAGGA AAAAAGAATT CATCATGGCG
    GAACTTCTAC AGACAGAGAA AGCGTATGTG CGGGACTTGC ACGAGTGTAT GGAGACCTAC
    CTGTGGGAAA TGACCAGCGG AGTGGAGGAA ATTCCGCCCG GAATAGTGAA TAAGGAGCAC
    ATCATCTTTG GGAACATTCA GGAAATATAC GACTTTCACA ATAACATCTT CCTGAAAGAG
    CTGGAGAAGT ACGAGCAGCT GCCGGAGGAC GTGGGACATT GTTTTGTCAC TTGGGCCGAC
    AAGTTCCACA TGTACGTGAC CTACTGTAAG AACAAACCCG ACTCCAGTCA GCTGATCCTG
    GAGCACGCCG GCTCCTTCTT TGACGAGATC CAGCAGCGAC ACGGCCTGGC CAACTCCATC
    TCCTCCTACC TCATCAAACC CGTCCAGCGG ATCACTAAGT ACCAGCTGCT TCTCAAGGAG
    CTTCTGACCT GCTGTGAGGA AGGGAAAGGG GAGATAAAGG ACGGGCTGGA GGTGATGCTG
    AGTGTGCCCA AGCGGGCCAA TGATGCCATG CACGTCAGCA TGCTGGAAGG ATTTGACGAA
    AACCTGGACG TTCAAGGGGA ACTTATCCTG CAAGATTCCT TCCAAGTCTG GGACCCCAAA
    TCCCTCATCC GCAAGGGGCG CGACCGGCAC CTCTTCCTCT TCGAAATCTC CCTCGTCTTC
    AGCAAAGAGA TCAAAGACTC CACCGGCCAC ACCAAATACG TCTTTAAGAA CAAACTGCTG
    ACCTCGGAAC TGGGGGTGAC GGAGCACGTA GAGGGGGACC CCTGTAAGTT TGCCCTATGG
    TCGGGGCGCA CCCCATCATC CGATAACAAG ACAGTGCTAA AGGCATCTAG TATCGAGGTG
    AAGCAGGAAT GGATCAAGAA CATCCGGGAA GTCATTCAGG AAAGGATGAT CCATTTGAAA
    GGAGCCTTGA AAGAACCCAT CCAGCCCCCC AAAACTCCAG CAAAGCAGCG CAACAACAGC
    AAAAGGGACG TACTGGAGGA CGCCGATAGC CAGGGGGACG GCAGCAGCCA ACCGGACACC
    ATCTCCATCG CCTCCAGAAC CTCCCAGAAC ACCATGGACA GCGACAAGTT ATCTGGGGGC
    TGTGAACTGA CGACGGTTCT GCAGGATTTC ATCGCTCTCC ACAACAATGA GCTGAGCATT
    CAGGTGGGGC AAACCGTGGA GCTGCTGGAG AGGCCGAGCG ACCGGCCTGG ATGGTGCTTA
    GTCCGGACCA CCGACCGGAG CCCCCCGCAG GAAGGGCTGG TACCCAGCAG CGCCCTGTGC
    ATCTCCCACT CGCGGAGCAG CGTCGAAATG GAGTGTTTCT TCCCTTCAGG CAAAGATTCA
    TTTTCTGTCT CCGCGGCGGA AAATGGGGGG AAGTCCGAGT CGGTGGCCAA TCTGCAGCCC
    CAGGCGTCCC TGAATTCTAT CCACAGCTCC CCGGGCCCCA AACGCTCCAC CAACACCCTA
    AAAAAGTGGC TGACGAGCCC TGTGAGGCGA CTGAACAGCG GGAAGGGCGA GAGCAACATC
    AAGAAGCAAA AGAAAAGAGA CGGGCGCAAC CTCGGCTCCC CCAGAGCTGG GGACGAGACC
    ACCCCACAGG GAGACAGCGC CGATGAGAAG GGCAAGAAGG GGTGGGACGA AGAGGCTGAC
    GACGAATCCC ACACTCCGCT TCCTCCGCCA ATGAAGATCT TTGATAATGA CCCCACTCCG
    GACGACAAGA CCATTGACGG AGGTTCTTAT CGGGGCGCAC AGAACATAAA TGAAGGATTT
    GTGAATGAGG GTTTGGCCCC CTCGCCAGCT CCCAGGAACC ACGAAGAGGA GCAAAAAGCC
    AAAGCACTCA GGGGCAGGAT GTTTGTACTG AATGAGCTGG TACAGACGGA GAAGGACTAT
    GTCCGGGATC TTGGGTTTAT GGTAGAGACG TACATCCCCA AGATGGAGGA GAGGGGGACC
    CCGGAAGATA TGAACGGAAA AGACAAGATC GTATTCGGCA ACATCCACCA GATCTACGAT
    TGGCACAAAG ACTTTTACTT GGCAGAACTA GAGAAATGCC TATTGGAGCC GGAGAGGTTG
    GCGCAGTTAT TTATAAAACA TGAGAGGCGG CTGCACATGT ATGTGGTTTA TTGCCAAAAC
    AAACCGCGCT CGGAGTTTGT CGTTGCCGAA TATGACGCCT ACTTTGAGGA TCTCATGCAG
    GAGGTGAATC CCAGATTTAC TGTCAGTGAT TTTCTCATCA AACCGATCCA AAGGATAACT
    AAGTACCAAC TGCTGCTAAA GGACTTCCTT AAGTACAGTC AGAAGGCGGG TTTGGAGTGT
    TCAGAAATCG AGAAAGCCGT GGAACTAATG TGCCTTGTCC CGAAACGCTG CAACGACATG
    ATGAACCTGG GGCGCCTGCA GGGATTCGAG GGGAAGCTGA CAGCCCAGGG GAAGCTGTTG
    CAGCAGGACA CCTTCTATGT GATCGAGCAG GAATCGGGGG TCCAGTCTCG GACGAAGGAA
    AGGCGGGTTT TCCTGTTCGA GCAGATTGTT ATTTTCAGCG AGCTCCTGCG GAAAGGTTCC
    TCCACGCCGG GGTACATGTT CAAAAGGGGC ATAAAGATGA ATTATCTCAT CTTAGAAGAG
    AACATTGACA ACGACCCGTG CAAATTCGCC ATTCGGAGCC GCGAGACGTC GGAGCGGGTG
    ATCCTGCAAG CGGCCAACGC CGACATTAAA CAGGCCTGGG TGCAGGACAT CGGGCAAGTG
    CTGGAGACCC AGAGGGACTT CCTAAACGCC CTGCAATCGC CAATAGAATA CCAGCGGAAG
    GAGAGCAACG CAGCCATGAA CAAGCCTCAG ATTGGCCGCG GGCCGTCCCC CACCGTTAGG
    CCTCTCTCCT CTACGCCCGT AGGGTGTGAA AAAGATGTCA CCATCCCAGT GCGGAACCAG
    TCGCTACCAG CGCGGAAAAT ACCTACCTCT AACGGCAGCG CCGGGTATGA AATGTACCCA
    CGGGCGGAGA AATGTGACCC AAACCAGAGC GAGCTGAGCG GCTGCAATGG GACCAACTCC
    ATGGTGGTTG TACAGGATTA CTATGCACTA AAGGAGAATG AGATCTGCGT GTACCACGGG
    GAGTTGGTAC AGATTCTGGC CATCAATCAG CAGAACATGT TCCTGGTATT CCGGCCGTCC
    AATGACCATT CCCCGGCAGC GGAAGGCTGG ATCCCGGGAT CAGTTTTGGG CCACATTTCT
    AAACCTTCCG CAGAAAATAG CGACAGATCC ATCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748501.1
    CDS16..4374
    Translation

    Target ORF information:

    RefSeq Version XM_031892641.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kalirin RhoGEF kinase (kalrn), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892641.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGGCGAGT CGATGCTGAA TGCCAGTCTG GTTAATGCCA GTTCACTGTC GGAAGCTGAG 
    CAGTTGCAGC GAGAACACGA GCAGTTTCAG ATGGCTATTG AGTCCCTTTT CCACGCCAGC
    TCTCTGCAGA AGACTCACCA AAGTGCCCTG CAGGTCCAGC AGAAAGCAGA GGCGCTTCTA
    CAGGCCGGGC ACTACGACGC CGACGCCATC CGGGAATGCG CCGAGAAGGT GGCGATGCAC
    TGGCAGCAAC TCATGCTCAA GATGGAGGAC AGACTCAAGC TAGTGAATGC CTCTGTGGCC
    TTCTATAAAA CCTCCGAGCA GGTGTGCAGC GTTCTGGAGA GCCTGGAGCA GGAGTACCGC
    AGGGATGAGG ACTGGTGTCG GGGCCACGAT AAGCTGGGAC CCTCCACTGA GTCGGACCAC
    GTGATGCCGC TGATCAGTAA ACACCTGGAG CAGAAGGAGG CTTTCCTTAA GGCTTGTACG
    TTAGCGCGGA GGAACGCGGA AGTCTTTCTG AAGTACATTC ACCGTAACAA CGTGAGCATG
    CCCGGCGTGG CCCCCCACAC GCGCGTCCCC GAGCAGCAAG TTAAAGCCAT ACTGAGCGAG
    CTCCTGCAAA GGGAGAACCG GGTGCTGCAC TTTTGGACCC TGAAGAAGCG GCGGTTGGAC
    CAGTGCCAGC AGTACATTGT GTTTGAGAGG AGCGCCAAGC AGGCTCTGGA CTGGATTCAG
    GAAGCCGGGG AGCATTATCT CTCCACTCGC ATCTCCCCTG GGGAATGTAT TGAGGAGACT
    CAGGAGCTGC TGAAGGAATA CGAGGAATTC CGGATACCTG CCAAGCAAAC GAAGGAGAAG
    GTGCGGCTGC TGATCCAGCT GGCAGACAGC TTTGTGGAGA AGGGCCACGT TCACGCCACC
    GAGCTGAAGA AATGGGTCAC TACTGTCAAC AAACACTACC GGGAATTCGC TCTGCGTATG
    GGCAAATACC ATTGCGGCCT GCAGCGAGCC CTGGGCATCT GCACTGAGGA CACAAAGGAC
    CTGGAGTTGG ACATTGTCCC TGCGATTCTG ACTGACCCGG AGGTGAAGCT GCGCGACGCC
    GGCCATGAAC TCAACGAGGA GAAGAGAAAA TCCGCCAGGA AAAAAGAATT CATCATGGCG
    GAACTTCTAC AGACAGAGAA AGCGTATGTG CGGGACTTGC ACGAGTGTAT GGAGACCTAC
    CTGTGGGAAA TGACCAGCGG AGTGGAGGAA ATTCCGCCCG GAATAGTGAA TAAGGAGCAC
    ATCATCTTTG GGAACATTCA GGAAATATAC GACTTTCACA ATAACATCTT CCTGAAAGAG
    CTGGAGAAGT ACGAGCAGCT GCCGGAGGAC GTGGGACATT GTTTTGTCAC TTGGGCCGAC
    AAGTTCCACA TGTACGTGAC CTACTGTAAG AACAAACCCG ACTCCAGTCA GCTGATCCTG
    GAGCACGCCG GCTCCTTCTT TGACGAGATC CAGCAGCGAC ACGGCCTGGC CAACTCCATC
    TCCTCCTACC TCATCAAACC CGTCCAGCGG ATCACTAAGT ACCAGCTGCT TCTCAAGGAG
    CTTCTGACCT GCTGTGAGGA AGGGAAAGGG GAGATAAAGG ACGGGCTGGA GGTGATGCTG
    AGTGTGCCCA AGCGGGCCAA TGATGCCATG CACGTCAGCA TGCTGGAAGG ATTTGACGAA
    AACCTGGACG TTCAAGGGGA ACTTATCCTG CAAGATTCCT TCCAAGTCTG GGACCCCAAA
    TCCCTCATCC GCAAGGGGCG CGACCGGCAC CTCTTCCTCT TCGAAATCTC CCTCGTCTTC
    AGCAAAGAGA TCAAAGACTC CACCGGCCAC ACCAAATACG TCTTTAAGAA CAAACTGCTG
    ACCTCGGAAC TGGGGGTGAC GGAGCACGTA GAGGGGGACC CCTGTAAGTT TGCCCTATGG
    TCGGGGCGCA CCCCATCATC CGATAACAAG ACAGTGCTAA AGGCATCTAG TATCGAGGTG
    AAGCAGGAAT GGATCAAGAA CATCCGGGAA GTCATTCAGG AAAGGATGAT CCATTTGAAA
    GGAGCCTTGA AAGAACCCAT CCAGCCCCCC AAAACTCCAG CAAAGCAGCG CAACAACAGC
    AAAAGGGACG TACTGGAGGA CGCCGATAGC CAGGGGGACG GCAGCAGCCA ACCGGACACC
    ATCTCCATCG CCTCCAGAAC CTCCCAGAAC ACCATGGACA GCGACAAGTT ATCTGGGGGC
    TGTGAACTGA CGACGGTTCT GCAGGATTTC ATCGCTCTCC ACAACAATGA GCTGAGCATT
    CAGGTGGGGC AAACCGTGGA GCTGCTGGAG AGGCCGAGCG ACCGGCCTGG ATGGTGCTTA
    GTCCGGACCA CCGACCGGAG CCCCCCGCAG GAAGGGCTGG TACCCAGCAG CGCCCTGTGC
    ATCTCCCACT CGCGGAGCAG CGTCGAAATG GAGTGTTTCT TCCCTTCAGG CAAAGATTCA
    TTTTCTGTCT CCGCGGCGGA AAATGGGGGG AAGTCCGAGT CGGTGGCCAA TCTGCAGCCC
    CAGGCGTCCC TGAATTCTAT CCACAGCTCC CCGGGCCCCA AACGCTCCAC CAACACCCTA
    AAAAAGTGGC TGACGAGCCC TGTGAGGCGA CTGAACAGCG GGAAGGGCGA GAGCAACATC
    AAGAAGCAAA AGAAAAGAGA CGGGCGCAAC CTCGGCTCCC CCAGAGCTGG GGACGAGACC
    ACCCCACAGG GAGACAGCGC CGATGAGAAG GGCAAGAAGG GGTGGGACGA AGAGGCTGAC
    GACGAATCCC ACACTCCGCT TCCTCCGCCA ATGAAGATCT TTGATAATGA CCCCACTCCG
    GACGACAAGA CCATTGACGG AGGTTCTTAT CGGGGCGCAC AGAACATAAA TGAAGGATTT
    GTGAATGAGG GTTTGGCCCC CTCGCCAGCT CCCAGGAACC ACGAAGAGGA GCAAAAAGCC
    AAAGCACTCA GGGGCAGGAT GTTTGTACTG AATGAGCTGG TACAGACGGA GAAGGACTAT
    GTCCGGGATC TTGGGTTTAT GGTAGAGACG TACATCCCCA AGATGGAGGA GAGGGGGACC
    CCGGAAGATA TGAACGGAAA AGACAAGATC GTATTCGGCA ACATCCACCA GATCTACGAT
    TGGCACAAAG ACTTTTACTT GGCAGAACTA GAGAAATGCC TATTGGAGCC GGAGAGGTTG
    GCGCAGTTAT TTATAAAACA TGAGAGGCGG CTGCACATGT ATGTGGTTTA TTGCCAAAAC
    AAACCGCGCT CGGAGTTTGT CGTTGCCGAA TATGACGCCT ACTTTGAGGA TCTCATGCAG
    GAGGTGAATC CCAGATTTAC TGTCAGTGAT TTTCTCATCA AACCGATCCA AAGGATAACT
    AAGTACCAAC TGCTGCTAAA GGACTTCCTT AAGTACAGTC AGAAGGCGGG TTTGGAGTGT
    TCAGAAATCG AGAAAGCCGT GGAACTAATG TGCCTTGTCC CGAAACGCTG CAACGACATG
    ATGAACCTGG GGCGCCTGCA GGGATTCGAG GGGAAGCTGA CAGCCCAGGG GAAGCTGTTG
    CAGCAGGACA CCTTCTATGT GATCGAGCAG GAATCGGGGG TCCAGTCTCG GACGAAGGAA
    AGGCGGGTTT TCCTGTTCGA GCAGATTGTT ATTTTCAGCG AGCTCCTGCG GAAAGGTTCC
    TCCACGCCGG GGTACATGTT CAAAAGGGGC ATAAAGATGA ATTATCTCAT CTTAGAAGAG
    AACATTGACA ACGACCCGTG CAAATTCGCC ATTCGGAGCC GCGAGACGTC GGAGCGGGTG
    ATCCTGCAAG CGGCCAACGC CGACATTAAA CAGGCCTGGG TGCAGGACAT CGGGCAAGTG
    CTGGAGACCC AGAGGGACTT CCTAAACGCC CTGCAATCGC CAATAGAATA CCAGCGGAAG
    GAGAGCAACG CAGCCATGAA CAAGCCTCAG ATTGGCCGCG GGCCGTCCCC CACCGTTAGG
    CCTCTCTCCT CTACGCCCGT AGGGTGTGAA AAAGATGTCA CCATCCCAGT GCGGAACCAG
    TCGCTACCAG CGCGGAAAAT ACCTACCTCT AACGGCAGCG CCGGGTATGA AATGTACCCA
    CGGGCGGAGA AATGTGACCC AAACCAGAGC GAGCTGAGCG GCTGCAATGG GACCAACTCC
    ATGGTGGTTG TACAGGATTA CTATGCACTA AAGGAGAATG AGATCTGCGT GTACCACGGG
    GAGTTGGTAC AGATTCTGGC CATCAATCAG CAGAACATGT TCCTGGTATT CCGGCCGTCC
    AATGACCATT CCCCGGCAGC GGAAGGCTGG ATCCCGGGAT CAGTTTTGGG CCACATTTCT
    AAACCTTCCG CAGAAAATAG CGACAGATCC ATCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa66023
    Clone ID Related Accession (Same CDS sequence) XM_031892642.1
    Accession Version XM_031892642.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kalirin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGGCGAGT CGATGCTGAA TGCCAGTCTG GTTAATGCCA GTTCACTGTC GGAAGCTGAG 
    CAGTTGCAGC GAGAACACGA GCAGTTTCAG ATGGCTATTG AGTCCCTTTT CCACGCCAGC
    TCTCTGCAGA AGACTCACCA AAGTGCCCTG CAGGTCCAGC AGAAAGCAGA GGCGCTTCTA
    CAGGCCGGGC ACTACGACGC CGACGCCATC CGGGAATGCG CCGAGAAGGT GGCGATGCAC
    TGGCAGCAAC TCATGCTCAA GATGGAGGAC AGACTCAAGC TAGTGAATGC CTCTGTGGCC
    TTCTATAAAA CCTCCGAGCA GGTGTGCAGC GTTCTGGAGA GCCTGGAGCA GGAGTACCGC
    AGGGATGAGG ACTGGTGTCG GGGCCACGAT AAGCTGGGAC CCTCCACTGA GTCGGACCAC
    GTGATGCCGC TGATCAGTAA ACACCTGGAG CAGAAGGAGG CTTTCCTTAA GGCTTGTACG
    TTAGCGCGGA GGAACGCGGA AGTCTTTCTG AAGTACATTC ACCGTAACAA CGTGAGCATG
    CCCGGCGTGG CCCCCCACAC GCGCGTCCCC GAGCAGCAAG TTAAAGCCAT ACTGAGCGAG
    CTCCTGCAAA GGGAGAACCG GGTGCTGCAC TTTTGGACCC TGAAGAAGCG GCGGTTGGAC
    CAGTGCCAGC AGTACATTGT GTTTGAGAGG AGCGCCAAGC AGGCTCTGGA CTGGATTCAG
    GAAGCCGGGG AGCATTATCT CTCCACTCGC ATCTCCCCTG GGGAATGTAT TGAGGAGACT
    CAGGAGCTGC TGAAGGAATA CGAGGAATTC CGGATACCTG CCAAGCAAAC GAAGGAGAAG
    GTGCGGCTGC TGATCCAGCT GGCAGACAGC TTTGTGGAGA AGGGCCACGT TCACGCCACC
    GAGCTGAAGA AATGGGTCAC TACTGTCAAC AAACACTACC GGGAATTCGC TCTGCGTATG
    GGCAAATACC ATTGCGGCCT GCAGCGAGCC CTGGGCATCT GCACTGAGGA CACAAAGGAC
    CTGGAGTTGG ACATTGTCCC TGCGATTCTG ACTGACCCGG AGGTGAAGCT GCGCGACGCC
    GGCCATGAAC TCAACGAGGA GAAGAGAAAA TCCGCCAGGA AAAAAGAATT CATCATGGCG
    GAACTTCTAC AGACAGAGAA AGCGTATGTG CGGGACTTGC ACGAGTGTAT GGAGACCTAC
    CTGTGGGAAA TGACCAGCGG AGTGGAGGAA ATTCCGCCCG GAATAGTGAA TAAGGAGCAC
    ATCATCTTTG GGAACATTCA GGAAATATAC GACTTTCACA ATAACATCTT CCTGAAAGAG
    CTGGAGAAGT ACGAGCAGCT GCCGGAGGAC GTGGGACATT GTTTTGTCAC TTGGGCCGAC
    AAGTTCCACA TGTACGTGAC CTACTGTAAG AACAAACCCG ACTCCAGTCA GCTGATCCTG
    GAGCACGCCG GCTCCTTCTT TGACGAGATC CAGCAGCGAC ACGGCCTGGC CAACTCCATC
    TCCTCCTACC TCATCAAACC CGTCCAGCGG ATCACTAAGT ACCAGCTGCT TCTCAAGGAG
    CTTCTGACCT GCTGTGAGGA AGGGAAAGGG GAGATAAAGG ACGGGCTGGA GGTGATGCTG
    AGTGTGCCCA AGCGGGCCAA TGATGCCATG CACGTCAGCA TGCTGGAAGG ATTTGACGAA
    AACCTGGACG TTCAAGGGGA ACTTATCCTG CAAGATTCCT TCCAAGTCTG GGACCCCAAA
    TCCCTCATCC GCAAGGGGCG CGACCGGCAC CTCTTCCTCT TCGAAATCTC CCTCGTCTTC
    AGCAAAGAGA TCAAAGACTC CACCGGCCAC ACCAAATACG TCTTTAAGAA CAAACTGCTG
    ACCTCGGAAC TGGGGGTGAC GGAGCACGTA GAGGGGGACC CCTGTAAGTT TGCCCTATGG
    TCGGGGCGCA CCCCATCATC CGATAACAAG ACAGTGCTAA AGGCATCTAG TATCGAGGTG
    AAGCAGGAAT GGATCAAGAA CATCCGGGAA GTCATTCAGG AAAGGATGAT CCATTTGAAA
    GGAGCCTTGA AAGAACCCAT CCAGCCCCCC AAAACTCCAG CAAAGCAGCG CAACAACAGC
    AAAAGGGACG TACTGGAGGA CGCCGATAGC CAGGGGGACG GCAGCAGCCA ACCGGACACC
    ATCTCCATCG CCTCCAGAAC CTCCCAGAAC ACCATGGACA GCGACAAGTT ATCTGGGGGC
    TGTGAACTGA CGACGGTTCT GCAGGATTTC ATCGCTCTCC ACAACAATGA GCTGAGCATT
    CAGGTGGGGC AAACCGTGGA GCTGCTGGAG AGGCCGAGCG ACCGGCCTGG ATGGTGCTTA
    GTCCGGACCA CCGACCGGAG CCCCCCGCAG GAAGGGCTGG TACCCAGCAG CGCCCTGTGC
    ATCTCCCACT CGCGGAGCAG CGTCGAAATG GAGTGTTTCT TCCCTTCAGG CAAAGATTCA
    TTTTCTGTCT CCGCGGCGGA AAATGGGGGG AAGTCCGAGT CGGTGGCCAA TCTGCAGCCC
    CAGGCGTCCC TGAATTCTAT CCACAGCTCC CCGGGCCCCA AACGCTCCAC CAACACCCTA
    AAAAAGTGGC TGACGAGCCC TGTGAGGCGA CTGAACAGCG GGAAGGGCGA GAGCAACATC
    AAGAAGCAAA AGAAAAGAGA CGGGCGCAAC CTCGGCTCCC CCAGAGCTGG GGACGAGACC
    ACCCCACAGG GAGACAGCGC CGATGAGGGC AAGAAGGGGT GGGACGAAGA GGCTGACGAC
    GAATCCCACA CTCCGCTTCC TCCGCCAATG AAGATCTTTG ATAATGACCC CACTCCGGAC
    GACAAGACCA TTGACGGAGG TTCTTATCGG GGCGCACAGA ACATAAATGA AGGATTTGTG
    AATGAGGGTT TGGCCCCCTC GCCAGCTCCC AGGAACCACG AAGAGGAGCA AAAAGCCAAA
    GCACTCAGGG GCAGGATGTT TGTACTGAAT GAGCTGGTAC AGACGGAGAA GGACTATGTC
    CGGGATCTTG GGTTTATGGT AGAGACGTAC ATCCCCAAGA TGGAGGAGAG GGGGACCCCG
    GAAGATATGA ACGGAAAAGA CAAGATCGTA TTCGGCAACA TCCACCAGAT CTACGATTGG
    CACAAAGACT TTTACTTGGC AGAACTAGAG AAATGCCTAT TGGAGCCGGA GAGGTTGGCG
    CAGTTATTTA TAAAACATGA GAGGCGGCTG CACATGTATG TGGTTTATTG CCAAAACAAA
    CCGCGCTCGG AGTTTGTCGT TGCCGAATAT GACGCCTACT TTGAGGATCT CATGCAGGAG
    GTGAATCCCA GATTTACTGT CAGTGATTTT CTCATCAAAC CGATCCAAAG GATAACTAAG
    TACCAACTGC TGCTAAAGGA CTTCCTTAAG TACAGTCAGA AGGCGGGTTT GGAGTGTTCA
    GAAATCGAGA AAGCCGTGGA ACTAATGTGC CTTGTCCCGA AACGCTGCAA CGACATGATG
    AACCTGGGGC GCCTGCAGGG ATTCGAGGGG AAGCTGACAG CCCAGGGGAA GCTGTTGCAG
    CAGGACACCT TCTATGTGAT CGAGCAGGAA TCGGGGGTCC AGTCTCGGAC GAAGGAAAGG
    CGGGTTTTCC TGTTCGAGCA GATTGTTATT TTCAGCGAGC TCCTGCGGAA AGGTTCCTCC
    ACGCCGGGGT ACATGTTCAA AAGGGGCATA AAGATGAATT ATCTCATCTT AGAAGAGAAC
    ATTGACAACG ACCCGTGCAA ATTCGCCATT CGGAGCCGCG AGACGTCGGA GCGGGTGATC
    CTGCAAGCGG CCAACGCCGA CATTAAACAG GCCTGGGTGC AGGACATCGG GCAAGTGCTG
    GAGACCCAGA GGGACTTCCT AAACGCCCTG CAATCGCCAA TAGAATACCA GCGGAAGGAG
    AGCAACGCAG CCATGAACAA GCCTCAGATT GGCCGCGGGC CGTCCCCCAC CGTTAGGCCT
    CTCTCCTCTA CGCCCGTAGG GTGTGAAAAA GATGTCACCA TCCCAGTGCG GAACCAGTCG
    CTACCAGCGC GGAAAATACC TACCTCTAAC GGCAGCGCCG GGTATGAAAT GTACCCACGG
    GCGGAGAAAT GTGACCCAAA CCAGAGCGAG CTGAGCGGCT GCAATGGGAC CAACTCCATG
    GTGGTTGTAC AGGATTACTA TGCACTAAAG GAGAATGAGA TCTGCGTGTA CCACGGGGAG
    TTGGTACAGA TTCTGGCCAT CAATCAGCAG AACATGTTCC TGGTATTCCG GCCGTCCAAT
    GACCATTCCC CGGCAGCGGA AGGCTGGATC CCGGGATCAG TTTTGGGCCA CATTTCTAAA
    CCTTCCGCAG AAAATAGCGA CAGATCCATC AAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748502.1
    CDS16..4371
    Translation

    Target ORF information:

    RefSeq Version XM_031892642.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kalirin RhoGEF kinase (kalrn), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892642.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGGCGAGT CGATGCTGAA TGCCAGTCTG GTTAATGCCA GTTCACTGTC GGAAGCTGAG 
    CAGTTGCAGC GAGAACACGA GCAGTTTCAG ATGGCTATTG AGTCCCTTTT CCACGCCAGC
    TCTCTGCAGA AGACTCACCA AAGTGCCCTG CAGGTCCAGC AGAAAGCAGA GGCGCTTCTA
    CAGGCCGGGC ACTACGACGC CGACGCCATC CGGGAATGCG CCGAGAAGGT GGCGATGCAC
    TGGCAGCAAC TCATGCTCAA GATGGAGGAC AGACTCAAGC TAGTGAATGC CTCTGTGGCC
    TTCTATAAAA CCTCCGAGCA GGTGTGCAGC GTTCTGGAGA GCCTGGAGCA GGAGTACCGC
    AGGGATGAGG ACTGGTGTCG GGGCCACGAT AAGCTGGGAC CCTCCACTGA GTCGGACCAC
    GTGATGCCGC TGATCAGTAA ACACCTGGAG CAGAAGGAGG CTTTCCTTAA GGCTTGTACG
    TTAGCGCGGA GGAACGCGGA AGTCTTTCTG AAGTACATTC ACCGTAACAA CGTGAGCATG
    CCCGGCGTGG CCCCCCACAC GCGCGTCCCC GAGCAGCAAG TTAAAGCCAT ACTGAGCGAG
    CTCCTGCAAA GGGAGAACCG GGTGCTGCAC TTTTGGACCC TGAAGAAGCG GCGGTTGGAC
    CAGTGCCAGC AGTACATTGT GTTTGAGAGG AGCGCCAAGC AGGCTCTGGA CTGGATTCAG
    GAAGCCGGGG AGCATTATCT CTCCACTCGC ATCTCCCCTG GGGAATGTAT TGAGGAGACT
    CAGGAGCTGC TGAAGGAATA CGAGGAATTC CGGATACCTG CCAAGCAAAC GAAGGAGAAG
    GTGCGGCTGC TGATCCAGCT GGCAGACAGC TTTGTGGAGA AGGGCCACGT TCACGCCACC
    GAGCTGAAGA AATGGGTCAC TACTGTCAAC AAACACTACC GGGAATTCGC TCTGCGTATG
    GGCAAATACC ATTGCGGCCT GCAGCGAGCC CTGGGCATCT GCACTGAGGA CACAAAGGAC
    CTGGAGTTGG ACATTGTCCC TGCGATTCTG ACTGACCCGG AGGTGAAGCT GCGCGACGCC
    GGCCATGAAC TCAACGAGGA GAAGAGAAAA TCCGCCAGGA AAAAAGAATT CATCATGGCG
    GAACTTCTAC AGACAGAGAA AGCGTATGTG CGGGACTTGC ACGAGTGTAT GGAGACCTAC
    CTGTGGGAAA TGACCAGCGG AGTGGAGGAA ATTCCGCCCG GAATAGTGAA TAAGGAGCAC
    ATCATCTTTG GGAACATTCA GGAAATATAC GACTTTCACA ATAACATCTT CCTGAAAGAG
    CTGGAGAAGT ACGAGCAGCT GCCGGAGGAC GTGGGACATT GTTTTGTCAC TTGGGCCGAC
    AAGTTCCACA TGTACGTGAC CTACTGTAAG AACAAACCCG ACTCCAGTCA GCTGATCCTG
    GAGCACGCCG GCTCCTTCTT TGACGAGATC CAGCAGCGAC ACGGCCTGGC CAACTCCATC
    TCCTCCTACC TCATCAAACC CGTCCAGCGG ATCACTAAGT ACCAGCTGCT TCTCAAGGAG
    CTTCTGACCT GCTGTGAGGA AGGGAAAGGG GAGATAAAGG ACGGGCTGGA GGTGATGCTG
    AGTGTGCCCA AGCGGGCCAA TGATGCCATG CACGTCAGCA TGCTGGAAGG ATTTGACGAA
    AACCTGGACG TTCAAGGGGA ACTTATCCTG CAAGATTCCT TCCAAGTCTG GGACCCCAAA
    TCCCTCATCC GCAAGGGGCG CGACCGGCAC CTCTTCCTCT TCGAAATCTC CCTCGTCTTC
    AGCAAAGAGA TCAAAGACTC CACCGGCCAC ACCAAATACG TCTTTAAGAA CAAACTGCTG
    ACCTCGGAAC TGGGGGTGAC GGAGCACGTA GAGGGGGACC CCTGTAAGTT TGCCCTATGG
    TCGGGGCGCA CCCCATCATC CGATAACAAG ACAGTGCTAA AGGCATCTAG TATCGAGGTG
    AAGCAGGAAT GGATCAAGAA CATCCGGGAA GTCATTCAGG AAAGGATGAT CCATTTGAAA
    GGAGCCTTGA AAGAACCCAT CCAGCCCCCC AAAACTCCAG CAAAGCAGCG CAACAACAGC
    AAAAGGGACG TACTGGAGGA CGCCGATAGC CAGGGGGACG GCAGCAGCCA ACCGGACACC
    ATCTCCATCG CCTCCAGAAC CTCCCAGAAC ACCATGGACA GCGACAAGTT ATCTGGGGGC
    TGTGAACTGA CGACGGTTCT GCAGGATTTC ATCGCTCTCC ACAACAATGA GCTGAGCATT
    CAGGTGGGGC AAACCGTGGA GCTGCTGGAG AGGCCGAGCG ACCGGCCTGG ATGGTGCTTA
    GTCCGGACCA CCGACCGGAG CCCCCCGCAG GAAGGGCTGG TACCCAGCAG CGCCCTGTGC
    ATCTCCCACT CGCGGAGCAG CGTCGAAATG GAGTGTTTCT TCCCTTCAGG CAAAGATTCA
    TTTTCTGTCT CCGCGGCGGA AAATGGGGGG AAGTCCGAGT CGGTGGCCAA TCTGCAGCCC
    CAGGCGTCCC TGAATTCTAT CCACAGCTCC CCGGGCCCCA AACGCTCCAC CAACACCCTA
    AAAAAGTGGC TGACGAGCCC TGTGAGGCGA CTGAACAGCG GGAAGGGCGA GAGCAACATC
    AAGAAGCAAA AGAAAAGAGA CGGGCGCAAC CTCGGCTCCC CCAGAGCTGG GGACGAGACC
    ACCCCACAGG GAGACAGCGC CGATGAGGGC AAGAAGGGGT GGGACGAAGA GGCTGACGAC
    GAATCCCACA CTCCGCTTCC TCCGCCAATG AAGATCTTTG ATAATGACCC CACTCCGGAC
    GACAAGACCA TTGACGGAGG TTCTTATCGG GGCGCACAGA ACATAAATGA AGGATTTGTG
    AATGAGGGTT TGGCCCCCTC GCCAGCTCCC AGGAACCACG AAGAGGAGCA AAAAGCCAAA
    GCACTCAGGG GCAGGATGTT TGTACTGAAT GAGCTGGTAC AGACGGAGAA GGACTATGTC
    CGGGATCTTG GGTTTATGGT AGAGACGTAC ATCCCCAAGA TGGAGGAGAG GGGGACCCCG
    GAAGATATGA ACGGAAAAGA CAAGATCGTA TTCGGCAACA TCCACCAGAT CTACGATTGG
    CACAAAGACT TTTACTTGGC AGAACTAGAG AAATGCCTAT TGGAGCCGGA GAGGTTGGCG
    CAGTTATTTA TAAAACATGA GAGGCGGCTG CACATGTATG TGGTTTATTG CCAAAACAAA
    CCGCGCTCGG AGTTTGTCGT TGCCGAATAT GACGCCTACT TTGAGGATCT CATGCAGGAG
    GTGAATCCCA GATTTACTGT CAGTGATTTT CTCATCAAAC CGATCCAAAG GATAACTAAG
    TACCAACTGC TGCTAAAGGA CTTCCTTAAG TACAGTCAGA AGGCGGGTTT GGAGTGTTCA
    GAAATCGAGA AAGCCGTGGA ACTAATGTGC CTTGTCCCGA AACGCTGCAA CGACATGATG
    AACCTGGGGC GCCTGCAGGG ATTCGAGGGG AAGCTGACAG CCCAGGGGAA GCTGTTGCAG
    CAGGACACCT TCTATGTGAT CGAGCAGGAA TCGGGGGTCC AGTCTCGGAC GAAGGAAAGG
    CGGGTTTTCC TGTTCGAGCA GATTGTTATT TTCAGCGAGC TCCTGCGGAA AGGTTCCTCC
    ACGCCGGGGT ACATGTTCAA AAGGGGCATA AAGATGAATT ATCTCATCTT AGAAGAGAAC
    ATTGACAACG ACCCGTGCAA ATTCGCCATT CGGAGCCGCG AGACGTCGGA GCGGGTGATC
    CTGCAAGCGG CCAACGCCGA CATTAAACAG GCCTGGGTGC AGGACATCGG GCAAGTGCTG
    GAGACCCAGA GGGACTTCCT AAACGCCCTG CAATCGCCAA TAGAATACCA GCGGAAGGAG
    AGCAACGCAG CCATGAACAA GCCTCAGATT GGCCGCGGGC CGTCCCCCAC CGTTAGGCCT
    CTCTCCTCTA CGCCCGTAGG GTGTGAAAAA GATGTCACCA TCCCAGTGCG GAACCAGTCG
    CTACCAGCGC GGAAAATACC TACCTCTAAC GGCAGCGCCG GGTATGAAAT GTACCCACGG
    GCGGAGAAAT GTGACCCAAA CCAGAGCGAG CTGAGCGGCT GCAATGGGAC CAACTCCATG
    GTGGTTGTAC AGGATTACTA TGCACTAAAG GAGAATGAGA TCTGCGTGTA CCACGGGGAG
    TTGGTACAGA TTCTGGCCAT CAATCAGCAG AACATGTTCC TGGTATTCCG GCCGTCCAAT
    GACCATTCCC CGGCAGCGGA AGGCTGGATC CCGGGATCAG TTTTGGGCCA CATTTCTAAA
    CCTTCCGCAG AAAATAGCGA CAGATCCATC AAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa66024
    Clone ID Related Accession (Same CDS sequence) XM_031892643.1
    Accession Version XM_031892643.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4020bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kalirin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGAAAGT GGCTGAAGAC AGATAAGTGG ATAAAATCTT TATCTGGGGG CTGTGAACTG 
    ACGACGGTTC TGCAGGATTT CATCGCTCTC CACAACAATG AGCTGAGCAT TCAGGTGGGG
    CAAACCGTGG AGCTGCTGGA GAGGCCGAGC GACCGGCCTG GATGGTGCTT AGTCCGGACC
    ACCGACCGGA GCCCCCCGCA GGAAGGGCTG GTACCCAGCA GCGCCCTGTG CATCTCCCAC
    TCGCGGAGCA GCGTCGAAAT GGAGTGTTTC TTCCCTTCAG GCAAAGATTC ATTTTCTGTC
    TCCGCGGCGG AAAATGGGGG GAAGTCCGAG TCGGTGGCCA ATCTGCAGCC CCAGGCGTCC
    CTGAATTCTA TCCACAGCTC CCCGGGCCCC AAACGCTCCA CCAACACCCT AAAAAAGTGG
    CTGACGAGCC CTGTGAGGCG ACTGAACAGC GGGAAGGGCG AGAGCAACAT CAAGAAGCAA
    AAGAAAAGAG ACGGGCGCAA CCTCGGCTCC CCCAGAGCTG GGGACGAGAC CACCCCACAG
    GGAGACAGCG CCGATGAGAA GGGCAAGAAG GGGTGGGACG AAGAGGCTGA CGACGAATCC
    CACACTCCGC TTCCTCCGCC AATGAAGATC TTTGATAATG ACCCCACTCC GGACGACAAG
    TCCTCCCTGC TTGTGGTCCG GCAGAGCTCA GCGGAGGTGC CTAGTGCGGC TGAACTGGTC
    ACTGCCATAG AGAATCTGGT CAGGAACAAG ATGACCATTG ACGGAGGTTC TTATCGGGGC
    GCACAGAACA TAAATGAAGG ATTTGTGAAT GAGGGTTTGG CCCCCTCGCC AGCTCCCAGG
    AACCACGAAG AGGAGCAAAA AGCCAAAGCA CTCAGGGGCA GGATGTTTGT ACTGAATGAG
    CTGGTACAGA CGGAGAAGGA CTATGTCCGG GATCTTGGGT TTATGGTAGA GACGTACATC
    CCCAAGATGG AGGAGAGGGG GACCCCGGAA GATATGAACG GAAAAGACAA GATCGTATTC
    GGCAACATCC ACCAGATCTA CGATTGGCAC AAAGACTTTT ACTTGGCAGA ACTAGAGAAA
    TGCCTATTGG AGCCGGAGAG GTTGGCGCAG TTATTTATAA AACATGAGAG GCGGCTGCAC
    ATGTATGTGG TTTATTGCCA AAACAAACCG CGCTCGGAGT TTGTCGTTGC CGAATATGAC
    GCCTACTTTG AGGATCTCAT GCAGGAGGTG AATCCCAGAT TTACTGTCAG TGATTTTCTC
    ATCAAACCGA TCCAAAGGAT AACTAAGTAC CAACTGCTGC TAAAGGACTT CCTTAAGTAC
    AGTCAGAAGG CGGGTTTGGA GTGTTCAGAA ATCGAGAAAG CCGTGGAACT AATGTGCCTT
    GTCCCGAAAC GCTGCAACGA CATGATGAAC CTGGGGCGCC TGCAGGGATT CGAGGGGAAG
    CTGACAGCCC AGGGGAAGCT GTTGCAGCAG GACACCTTCT ATGTGATCGA GCAGGAATCG
    GGGGTCCAGT CTCGGACGAA GGAAAGGCGG GTTTTCCTGT TCGAGCAGAT TGTTATTTTC
    AGCGAGCTCC TGCGGAAAGG TTCCTCCACG CCGGGGTACA TGTTCAAAAG GGGCATAAAG
    ATGAATTATC TCATCTTAGA AGAGAACATT GACAACGACC CGTGCAAATT CGCCATTCGG
    AGCCGCGAGA CGTCGGAGCG GGTGATCCTG CAAGCGGCCA ACGCCGACAT TAAACAGGCC
    TGGGTGCAGG ACATCGGGCA AGTGCTGGAG ACCCAGAGGG ACTTCCTAAA CGCCCTGCAA
    TCGCCAATAG AATACCAGCG GAAGGAGAGC AACGCAGCCA TGAACAAGCC TCAGATTGGC
    CGCGGGCCGT CCCCCACCGT TAGGCCTCTC TCCTCTACGC CCGTAGGGTG TGAAAAAGAT
    GTCACCATCC CAGTGCGGAA CCAGTCGCTA CCAGCGCGGA AAATACCTAC CTCTAACGGC
    AGCGCCGGGT ATGAAATGTA CCCACGGGCG GAGAAATGTG ACCCAAACCA GAGCGAGCTG
    AGCGGCTGCA ATGGGACCAA CTCCATGGTG GTTGTACAGG ATTACTATGC ACTAAAGGAG
    AATGAGATCT GCGTGTACCA CGGGGAGTTG GTACAGATTC TGGCCATCAA TCAGCAGAAC
    ATGTTCCTGG TATTCCGGCC GTCCAATGAC CATTCCCCGG CAGCGGAAGG CTGGATCCCG
    GGATCAGTTT TGGGCCACAT TTCTAAACCT TCCGCAGAAA ATAGCGACAG ATCCATCAAG
    ACGTCATGCT CTTGGCACAC GCTCCGCATG AGAAAACGGG ACGGGACGGA GTCCAACGGC
    CCATGTAAGA GGCAAGAGGG CCTGGGCAGC ATGGCGAAAG ACCCCAATAA CAGTTCAGAG
    GAGTCTGAAT GCGATGACCT TGACCCCAGT GCTAGTATGG AGATAATGAA CCCTAATTTC
    ATCCAGGAAG TGGCCCCTGA GTTCCTGGTG TCCCTGGTGG ATGTCATGTG TGTTCTTGGG
    GACACAGTCA CCCTACAGTG CAAAGTGTGC GGCCGGCCGA AGCCCAGTGT CACCTGGAAG
    GGACCCGACC AGAGCATCTT GGATGGGAAC AGCAACACCT ACTCCATGTC AACCAGTGAG
    TCTGGAGACA TCAAGCTGAA GATCTGCCAC GTGATGCCCC AGGATAGTGG CATCTACACG
    TGTGTGGCCA GTAATGAGCT GGGGGCAACC TCGACATCTG CCACCATCAA AGTGCAAGGG
    GTCCCTGGGG CCCCCAGCCA GCCGCTGGCT CAGGAGAGAA GCAGCACCTC CGTCATCCTC
    CGCTGGCTGC CACCTACCAG CACCGGCAAT TGCACTATAT CCGGCTACAC TGTGGAATAC
    AAGGAGGAGG GTGCCCAACT ATGGCAGCAG TCAGTCGCCT CTACCCTGGA TACTTACCTC
    GTCCTAGAAG ACCTGTGCCC TGGGGCCCGG TACCAGTTCC GCGTGAGCGC CAGTAACCCC
    TGGGGTATAA GTCTGCCGAG TGAGCCTTCC GAGCCAGTCA GGCTTCCAGA AACTGATACC
    AATTCCAACG GATCAACAAT TTCATGGAAA GGAAACTTTG AGCAAGTCTA CACAGAGCTG
    CATGAGATTG GAAGGGGCAG GTTCTCCATA GTGAAGAAAT GTGCCAAGAA ACAGAGCAGG
    AAGGACGTGG CGGCCAAATT CATCAGCAAG AAGCTAAAGA AGCGAGAGCA GGCGGCGCAC
    GAGGCGACAC TTCTCCAGCA CCTACAGCAC CCACAGTACA TTACCATCCA TGACACCTTT
    GAGTCGGACG GTGCCTACAT CCTCATCCTG GACCTCATGG AAGATGGCCG CTTGCTGGAT
    TACCTCATGA AACACGACGA GCTGATGGAA GAGAAAGTGG CGTTCTACAT GCGAGACATC
    ATGGAGGCGC TGCAGTACCT GCACAGCTGC CGCGTGGCGC ATTTAGACAT CAAGCCGGAG
    AATCTCCTCA TAGACCTGCG CATCCCGGTG CCCAGAGTGA AGATCATCGA CCTGGAGGAC
    GCCGTGCAGA TAACGGGGCA CCATTATATC CATCAGCTGC TGGGGAACCC CGAGTTTGCT
    GCCCCCGAGC TTATCCAAGG GAGCCCCGTC TCTCTGGGCA CAGACATCTG GAGCTTGGGG
    GTGCTGACCT ACGTCATGCT GAGCGGGGTC TCCCCGTTCC TGGACGAGAG CAGCGAGGAG
    ACCTGCGCCA ACATCTGCCG GGTGGACTTC AGTTTCCCCC AGGAGTATTT CTCGGGGGTC
    AGTCAGGCTG CCCAGGACTT CATCAAGGCC ACGTTGCAAG GGGACCCTCG CTGGAGACCC
    ACCATCGCCA CCTGTCTGCA GCACCCATGG CTGCAGCCTC ATAACAACAC TTACTCCAAA
    TTACCCCTGG ACACCTCCCG CCTGGCTGGC TTCATTGAGA GGCGGAGCCA TCAAAACGAC
    GCCCAGCCAT TGGTCAAAAC CAAGAGTCTC ATCATTAGCA GGAACGTGGT GGGAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748503.1
    CDS218..4237
    Translation

    Target ORF information:

    RefSeq Version XM_031892643.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kalirin RhoGEF kinase (kalrn), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892643.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGAAAGT GGCTGAAGAC AGATAAGTGG ATAAAATCTT TATCTGGGGG CTGTGAACTG 
    ACGACGGTTC TGCAGGATTT CATCGCTCTC CACAACAATG AGCTGAGCAT TCAGGTGGGG
    CAAACCGTGG AGCTGCTGGA GAGGCCGAGC GACCGGCCTG GATGGTGCTT AGTCCGGACC
    ACCGACCGGA GCCCCCCGCA GGAAGGGCTG GTACCCAGCA GCGCCCTGTG CATCTCCCAC
    TCGCGGAGCA GCGTCGAAAT GGAGTGTTTC TTCCCTTCAG GCAAAGATTC ATTTTCTGTC
    TCCGCGGCGG AAAATGGGGG GAAGTCCGAG TCGGTGGCCA ATCTGCAGCC CCAGGCGTCC
    CTGAATTCTA TCCACAGCTC CCCGGGCCCC AAACGCTCCA CCAACACCCT AAAAAAGTGG
    CTGACGAGCC CTGTGAGGCG ACTGAACAGC GGGAAGGGCG AGAGCAACAT CAAGAAGCAA
    AAGAAAAGAG ACGGGCGCAA CCTCGGCTCC CCCAGAGCTG GGGACGAGAC CACCCCACAG
    GGAGACAGCG CCGATGAGAA GGGCAAGAAG GGGTGGGACG AAGAGGCTGA CGACGAATCC
    CACACTCCGC TTCCTCCGCC AATGAAGATC TTTGATAATG ACCCCACTCC GGACGACAAG
    TCCTCCCTGC TTGTGGTCCG GCAGAGCTCA GCGGAGGTGC CTAGTGCGGC TGAACTGGTC
    ACTGCCATAG AGAATCTGGT CAGGAACAAG ATGACCATTG ACGGAGGTTC TTATCGGGGC
    GCACAGAACA TAAATGAAGG ATTTGTGAAT GAGGGTTTGG CCCCCTCGCC AGCTCCCAGG
    AACCACGAAG AGGAGCAAAA AGCCAAAGCA CTCAGGGGCA GGATGTTTGT ACTGAATGAG
    CTGGTACAGA CGGAGAAGGA CTATGTCCGG GATCTTGGGT TTATGGTAGA GACGTACATC
    CCCAAGATGG AGGAGAGGGG GACCCCGGAA GATATGAACG GAAAAGACAA GATCGTATTC
    GGCAACATCC ACCAGATCTA CGATTGGCAC AAAGACTTTT ACTTGGCAGA ACTAGAGAAA
    TGCCTATTGG AGCCGGAGAG GTTGGCGCAG TTATTTATAA AACATGAGAG GCGGCTGCAC
    ATGTATGTGG TTTATTGCCA AAACAAACCG CGCTCGGAGT TTGTCGTTGC CGAATATGAC
    GCCTACTTTG AGGATCTCAT GCAGGAGGTG AATCCCAGAT TTACTGTCAG TGATTTTCTC
    ATCAAACCGA TCCAAAGGAT AACTAAGTAC CAACTGCTGC TAAAGGACTT CCTTAAGTAC
    AGTCAGAAGG CGGGTTTGGA GTGTTCAGAA ATCGAGAAAG CCGTGGAACT AATGTGCCTT
    GTCCCGAAAC GCTGCAACGA CATGATGAAC CTGGGGCGCC TGCAGGGATT CGAGGGGAAG
    CTGACAGCCC AGGGGAAGCT GTTGCAGCAG GACACCTTCT ATGTGATCGA GCAGGAATCG
    GGGGTCCAGT CTCGGACGAA GGAAAGGCGG GTTTTCCTGT TCGAGCAGAT TGTTATTTTC
    AGCGAGCTCC TGCGGAAAGG TTCCTCCACG CCGGGGTACA TGTTCAAAAG GGGCATAAAG
    ATGAATTATC TCATCTTAGA AGAGAACATT GACAACGACC CGTGCAAATT CGCCATTCGG
    AGCCGCGAGA CGTCGGAGCG GGTGATCCTG CAAGCGGCCA ACGCCGACAT TAAACAGGCC
    TGGGTGCAGG ACATCGGGCA AGTGCTGGAG ACCCAGAGGG ACTTCCTAAA CGCCCTGCAA
    TCGCCAATAG AATACCAGCG GAAGGAGAGC AACGCAGCCA TGAACAAGCC TCAGATTGGC
    CGCGGGCCGT CCCCCACCGT TAGGCCTCTC TCCTCTACGC CCGTAGGGTG TGAAAAAGAT
    GTCACCATCC CAGTGCGGAA CCAGTCGCTA CCAGCGCGGA AAATACCTAC CTCTAACGGC
    AGCGCCGGGT ATGAAATGTA CCCACGGGCG GAGAAATGTG ACCCAAACCA GAGCGAGCTG
    AGCGGCTGCA ATGGGACCAA CTCCATGGTG GTTGTACAGG ATTACTATGC ACTAAAGGAG
    AATGAGATCT GCGTGTACCA CGGGGAGTTG GTACAGATTC TGGCCATCAA TCAGCAGAAC
    ATGTTCCTGG TATTCCGGCC GTCCAATGAC CATTCCCCGG CAGCGGAAGG CTGGATCCCG
    GGATCAGTTT TGGGCCACAT TTCTAAACCT TCCGCAGAAA ATAGCGACAG ATCCATCAAG
    ACGTCATGCT CTTGGCACAC GCTCCGCATG AGAAAACGGG ACGGGACGGA GTCCAACGGC
    CCATGTAAGA GGCAAGAGGG CCTGGGCAGC ATGGCGAAAG ACCCCAATAA CAGTTCAGAG
    GAGTCTGAAT GCGATGACCT TGACCCCAGT GCTAGTATGG AGATAATGAA CCCTAATTTC
    ATCCAGGAAG TGGCCCCTGA GTTCCTGGTG TCCCTGGTGG ATGTCATGTG TGTTCTTGGG
    GACACAGTCA CCCTACAGTG CAAAGTGTGC GGCCGGCCGA AGCCCAGTGT CACCTGGAAG
    GGACCCGACC AGAGCATCTT GGATGGGAAC AGCAACACCT ACTCCATGTC AACCAGTGAG
    TCTGGAGACA TCAAGCTGAA GATCTGCCAC GTGATGCCCC AGGATAGTGG CATCTACACG
    TGTGTGGCCA GTAATGAGCT GGGGGCAACC TCGACATCTG CCACCATCAA AGTGCAAGGG
    GTCCCTGGGG CCCCCAGCCA GCCGCTGGCT CAGGAGAGAA GCAGCACCTC CGTCATCCTC
    CGCTGGCTGC CACCTACCAG CACCGGCAAT TGCACTATAT CCGGCTACAC TGTGGAATAC
    AAGGAGGAGG GTGCCCAACT ATGGCAGCAG TCAGTCGCCT CTACCCTGGA TACTTACCTC
    GTCCTAGAAG ACCTGTGCCC TGGGGCCCGG TACCAGTTCC GCGTGAGCGC CAGTAACCCC
    TGGGGTATAA GTCTGCCGAG TGAGCCTTCC GAGCCAGTCA GGCTTCCAGA AACTGATACC
    AATTCCAACG GATCAACAAT TTCATGGAAA GGAAACTTTG AGCAAGTCTA CACAGAGCTG
    CATGAGATTG GAAGGGGCAG GTTCTCCATA GTGAAGAAAT GTGCCAAGAA ACAGAGCAGG
    AAGGACGTGG CGGCCAAATT CATCAGCAAG AAGCTAAAGA AGCGAGAGCA GGCGGCGCAC
    GAGGCGACAC TTCTCCAGCA CCTACAGCAC CCACAGTACA TTACCATCCA TGACACCTTT
    GAGTCGGACG GTGCCTACAT CCTCATCCTG GACCTCATGG AAGATGGCCG CTTGCTGGAT
    TACCTCATGA AACACGACGA GCTGATGGAA GAGAAAGTGG CGTTCTACAT GCGAGACATC
    ATGGAGGCGC TGCAGTACCT GCACAGCTGC CGCGTGGCGC ATTTAGACAT CAAGCCGGAG
    AATCTCCTCA TAGACCTGCG CATCCCGGTG CCCAGAGTGA AGATCATCGA CCTGGAGGAC
    GCCGTGCAGA TAACGGGGCA CCATTATATC CATCAGCTGC TGGGGAACCC CGAGTTTGCT
    GCCCCCGAGC TTATCCAAGG GAGCCCCGTC TCTCTGGGCA CAGACATCTG GAGCTTGGGG
    GTGCTGACCT ACGTCATGCT GAGCGGGGTC TCCCCGTTCC TGGACGAGAG CAGCGAGGAG
    ACCTGCGCCA ACATCTGCCG GGTGGACTTC AGTTTCCCCC AGGAGTATTT CTCGGGGGTC
    AGTCAGGCTG CCCAGGACTT CATCAAGGCC ACGTTGCAAG GGGACCCTCG CTGGAGACCC
    ACCATCGCCA CCTGTCTGCA GCACCCATGG CTGCAGCCTC ATAACAACAC TTACTCCAAA
    TTACCCCTGG ACACCTCCCG CCTGGCTGGC TTCATTGAGA GGCGGAGCCA TCAAAACGAC
    GCCCAGCCAT TGGTCAAAAC CAAGAGTCTC ATCATTAGCA GGAACGTGGT GGGAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa66025
    Clone ID Related Accession (Same CDS sequence) XM_031892644.1
    Accession Version XM_031892644.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kalirin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAGGGTG GCACTGCCAA GTACCCCTCG GGCTCCTGCC TGGGGTGGCT CTGTACCCGC 
    TGCTGCTGCT GCTTCTTTGC AACAGATTCA TTTTCTGTCT CCGCGGCGGA AAATGGGGGG
    AAGTCCGAGT CGGTGGCCAA TCTGCAGCCC CAGGCGTCCC TGAATTCTAT CCACAGCTCC
    CCGGGCCCCA AACGCTCCAC CAACACCCTA AAAAAGTGGC TGACGAGCCC TGTGAGGCGA
    CTGAACAGCG GGAAGGGCGA GAGCAACATC AAGAAGCAAA AGAAAAGAGA CGGGCGCAAC
    CTCGGCTCCC CCAGAGCTGG GGACGAGACC ACCCCACAGG GAGACAGCGC CGATGAGAAG
    GGCAAGAAGG GGTGGGACGA AGAGGCTGAC GACGAATCCC ACACTCCGCT TCCTCCGCCA
    ATGAAGATCT TTGATAATGA CCCCACTCCG GACGACAAGT CCTCCCTGCT TGTGGTCCGG
    CAGAGCTCAG CGGAGGTGCC TAGTGCGGCT GAACTGGTCA CTGCCATAGA GAATCTGGTC
    AGGAACAAGA TGACCATTGA CGGAGGTTCT TATCGGGGCG CACAGAACAT AAATGAAGGA
    TTTGTGAATG AGGGTTTGGC CCCCTCGCCA GCTCCCAGGA ACCACGAAGA GGAGCAAAAA
    GCCAAAGCAC TCAGGGGCAG GATGTTTGTA CTGAATGAGC TGGTACAGAC GGAGAAGGAC
    TATGTCCGGG ATCTTGGGTT TATGGTAGAG ACGTACATCC CCAAGATGGA GGAGAGGGGG
    ACCCCGGAAG ATATGAACGG AAAAGACAAG ATCGTATTCG GCAACATCCA CCAGATCTAC
    GATTGGCACA AAGACTTTTA CTTGGCAGAA CTAGAGAAAT GCCTATTGGA GCCGGAGAGG
    TTGGCGCAGT TATTTATAAA ACATGAGAGG CGGCTGCACA TGTATGTGGT TTATTGCCAA
    AACAAACCGC GCTCGGAGTT TGTCGTTGCC GAATATGACG CCTACTTTGA GGATCTCATG
    CAGGAGGTGA ATCCCAGATT TACTGTCAGT GATTTTCTCA TCAAACCGAT CCAAAGGATA
    ACTAAGTACC AACTGCTGCT AAAGGACTTC CTTAAGTACA GTCAGAAGGC GGGTTTGGAG
    TGTTCAGAAA TCGAGAAAGC CGTGGAACTA ATGTGCCTTG TCCCGAAACG CTGCAACGAC
    ATGATGAACC TGGGGCGCCT GCAGGGATTC GAGGGGAAGC TGACAGCCCA GGGGAAGCTG
    TTGCAGCAGG ACACCTTCTA TGTGATCGAG CAGGAATCGG GGGTCCAGTC TCGGACGAAG
    GAAAGGCGGG TTTTCCTGTT CGAGCAGATT GTTATTTTCA GCGAGCTCCT GCGGAAAGGT
    TCCTCCACGC CGGGGTACAT GTTCAAAAGG GGCATAAAGA TGAATTATCT CATCTTAGAA
    GAGAACATTG ACAACGACCC GTGCAAATTC GCCATTCGGA GCCGCGAGAC GTCGGAGCGG
    GTGATCCTGC AAGCGGCCAA CGCCGACATT AAACAGGCCT GGGTGCAGGA CATCGGGCAA
    GTGCTGGAGA CCCAGAGGGA CTTCCTAAAC GCCCTGCAAT CGCCAATAGA ATACCAGCGG
    AAGGAGAGCA ACGCAGCCAT GAACAAGCCT CAGATTGGCC GCGGGCCGTC CCCCACCGTT
    AGGCCTCTCT CCTCTACGCC CGTAGGGTGT GAAAAAGATG TCACCATCCC AGTGCGGAAC
    CAGTCGCTAC CAGCGCGGAA AATACCTACC TCTAACGGCA GCGCCGGGTA TGAAATGTAC
    CCACGGGCGG AGAAATGTGA CCCAAACCAG AGCGAGCTGA GCGGCTGCAA TGGGACCAAC
    TCCATGGTGG TTGTACAGGA TTACTATGCA CTAAAGGAGA ATGAGATCTG CGTGTACCAC
    GGGGAGTTGG TACAGATTCT GGCCATCAAT CAGCAGAACA TGTTCCTGGT ATTCCGGCCG
    TCCAATGACC ATTCCCCGGC AGCGGAAGGC TGGATCCCGG GATCAGTTTT GGGCCACATT
    TCTAAACCTT CCGCAGAAAA TAGCGACAGA TCCATCAAGA CGTCATGCTC TTGGCACACG
    CTCCGCATGA GAAAACGGGA CGGGACGGAG TCCAACGGCC CATGTAAGAG GCAAGAGGGC
    CTGGGCAGCA TGGCGAAAGA CCCCAATAAC AGTTCAGAGG AGTCTGAATG CGATGACCTT
    GACCCCAGTG CTAGTATGGA GATAATGAAC CCTAATTTCA TCCAGGAAGT GGCCCCTGAG
    TTCCTGGTGT CCCTGGTGGA TGTCATGTGT GTTCTTGGGG ACACAGTCAC CCTACAGTGC
    AAAGTGTGCG GCCGGCCGAA GCCCAGTGTC ACCTGGAAGG GACCCGACCA GAGCATCTTG
    GATGGGAACA GCAACACCTA CTCCATGTCA ACCAGTGAGT CTGGAGACAT CAAGCTGAAG
    ATCTGCCACG TGATGCCCCA GGATAGTGGC ATCTACACGT GTGTGGCCAG TAATGAGCTG
    GGGGCAACCT CGACATCTGC CACCATCAAA GTGCAAGGGG TCCCTGGGGC CCCCAGCCAG
    CCGCTGGCTC AGGAGAGAAG CAGCACCTCC GTCATCCTCC GCTGGCTGCC ACCTACCAGC
    ACCGGCAATT GCACTATATC CGGCTACACT GTGGAATACA AGGAGGAGGG TGCCCAACTA
    TGGCAGCAGT CAGTCGCCTC TACCCTGGAT ACTTACCTCG TCCTAGAAGA CCTGTGCCCT
    GGGGCCCGGT ACCAGTTCCG CGTGAGCGCC AGTAACCCCT GGGGTATAAG TCTGCCGAGT
    GAGCCTTCCG AGCCAGTCAG GCTTCCAGAA ACTGATACCA ATTCCAACGG ATCAACAATT
    TCATGGAAAG GAAACTTTGA GCAAGTCTAC ACAGAGCTGC ATGAGATTGG AAGGGGCAGG
    TTCTCCATAG TGAAGAAATG TGCCAAGAAA CAGAGCAGGA AGGACGTGGC GGCCAAATTC
    ATCAGCAAGA AGCTAAAGAA GCGAGAGCAG GCGGCGCACG AGGCGACACT TCTCCAGCAC
    CTACAGCACC CACAGTACAT TACCATCCAT GACACCTTTG AGTCGGACGG TGCCTACATC
    CTCATCCTGG ACCTCATGGA AGATGGCCGC TTGCTGGATT ACCTCATGAA ACACGACGAG
    CTGATGGAAG AGAAAGTGGC GTTCTACATG CGAGACATCA TGGAGGCGCT GCAGTACCTG
    CACAGCTGCC GCGTGGCGCA TTTAGACATC AAGCCGGAGA ATCTCCTCAT AGACCTGCGC
    ATCCCGGTGC CCAGAGTGAA GATCATCGAC CTGGAGGACG CCGTGCAGAT AACGGGGCAC
    CATTATATCC ATCAGCTGCT GGGGAACCCC GAGTTTGCTG CCCCCGAGCT TATCCAAGGG
    AGCCCCGTCT CTCTGGGCAC AGACATCTGG AGCTTGGGGG TGCTGACCTA CGTCATGCTG
    AGCGGGGTCT CCCCGTTCCT GGACGAGAGC AGCGAGGAGA CCTGCGCCAA CATCTGCCGG
    GTGGACTTCA GTTTCCCCCA GGAGTATTTC TCGGGGGTCA GTCAGGCTGC CCAGGACTTC
    ATCAAGGCCA CGTTGCAAGG GGACCCTCGC TGGAGACCCA CCATCGCCAC CTGTCTGCAG
    CACCCATGGC TGCAGCCTCA TAACAACACT TACTCCAAAT TACCCCTGGA CACCTCCCGC
    CTGGCTGGCT TCATTGAGAG GCGGAGCCAT CAAAACGACG CCCAGCCATT GGTCAAAACC
    AAGAGTCTCA TCATTAGCAG GAACGTGGTG GGAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748504.1
    CDS98..3916
    Translation

    Target ORF information:

    RefSeq Version XM_031892644.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kalirin RhoGEF kinase (kalrn), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892644.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAGGGTG GCACTGCCAA GTACCCCTCG GGCTCCTGCC TGGGGTGGCT CTGTACCCGC 
    TGCTGCTGCT GCTTCTTTGC AACAGATTCA TTTTCTGTCT CCGCGGCGGA AAATGGGGGG
    AAGTCCGAGT CGGTGGCCAA TCTGCAGCCC CAGGCGTCCC TGAATTCTAT CCACAGCTCC
    CCGGGCCCCA AACGCTCCAC CAACACCCTA AAAAAGTGGC TGACGAGCCC TGTGAGGCGA
    CTGAACAGCG GGAAGGGCGA GAGCAACATC AAGAAGCAAA AGAAAAGAGA CGGGCGCAAC
    CTCGGCTCCC CCAGAGCTGG GGACGAGACC ACCCCACAGG GAGACAGCGC CGATGAGAAG
    GGCAAGAAGG GGTGGGACGA AGAGGCTGAC GACGAATCCC ACACTCCGCT TCCTCCGCCA
    ATGAAGATCT TTGATAATGA CCCCACTCCG GACGACAAGT CCTCCCTGCT TGTGGTCCGG
    CAGAGCTCAG CGGAGGTGCC TAGTGCGGCT GAACTGGTCA CTGCCATAGA GAATCTGGTC
    AGGAACAAGA TGACCATTGA CGGAGGTTCT TATCGGGGCG CACAGAACAT AAATGAAGGA
    TTTGTGAATG AGGGTTTGGC CCCCTCGCCA GCTCCCAGGA ACCACGAAGA GGAGCAAAAA
    GCCAAAGCAC TCAGGGGCAG GATGTTTGTA CTGAATGAGC TGGTACAGAC GGAGAAGGAC
    TATGTCCGGG ATCTTGGGTT TATGGTAGAG ACGTACATCC CCAAGATGGA GGAGAGGGGG
    ACCCCGGAAG ATATGAACGG AAAAGACAAG ATCGTATTCG GCAACATCCA CCAGATCTAC
    GATTGGCACA AAGACTTTTA CTTGGCAGAA CTAGAGAAAT GCCTATTGGA GCCGGAGAGG
    TTGGCGCAGT TATTTATAAA ACATGAGAGG CGGCTGCACA TGTATGTGGT TTATTGCCAA
    AACAAACCGC GCTCGGAGTT TGTCGTTGCC GAATATGACG CCTACTTTGA GGATCTCATG
    CAGGAGGTGA ATCCCAGATT TACTGTCAGT GATTTTCTCA TCAAACCGAT CCAAAGGATA
    ACTAAGTACC AACTGCTGCT AAAGGACTTC CTTAAGTACA GTCAGAAGGC GGGTTTGGAG
    TGTTCAGAAA TCGAGAAAGC CGTGGAACTA ATGTGCCTTG TCCCGAAACG CTGCAACGAC
    ATGATGAACC TGGGGCGCCT GCAGGGATTC GAGGGGAAGC TGACAGCCCA GGGGAAGCTG
    TTGCAGCAGG ACACCTTCTA TGTGATCGAG CAGGAATCGG GGGTCCAGTC TCGGACGAAG
    GAAAGGCGGG TTTTCCTGTT CGAGCAGATT GTTATTTTCA GCGAGCTCCT GCGGAAAGGT
    TCCTCCACGC CGGGGTACAT GTTCAAAAGG GGCATAAAGA TGAATTATCT CATCTTAGAA
    GAGAACATTG ACAACGACCC GTGCAAATTC GCCATTCGGA GCCGCGAGAC GTCGGAGCGG
    GTGATCCTGC AAGCGGCCAA CGCCGACATT AAACAGGCCT GGGTGCAGGA CATCGGGCAA
    GTGCTGGAGA CCCAGAGGGA CTTCCTAAAC GCCCTGCAAT CGCCAATAGA ATACCAGCGG
    AAGGAGAGCA ACGCAGCCAT GAACAAGCCT CAGATTGGCC GCGGGCCGTC CCCCACCGTT
    AGGCCTCTCT CCTCTACGCC CGTAGGGTGT GAAAAAGATG TCACCATCCC AGTGCGGAAC
    CAGTCGCTAC CAGCGCGGAA AATACCTACC TCTAACGGCA GCGCCGGGTA TGAAATGTAC
    CCACGGGCGG AGAAATGTGA CCCAAACCAG AGCGAGCTGA GCGGCTGCAA TGGGACCAAC
    TCCATGGTGG TTGTACAGGA TTACTATGCA CTAAAGGAGA ATGAGATCTG CGTGTACCAC
    GGGGAGTTGG TACAGATTCT GGCCATCAAT CAGCAGAACA TGTTCCTGGT ATTCCGGCCG
    TCCAATGACC ATTCCCCGGC AGCGGAAGGC TGGATCCCGG GATCAGTTTT GGGCCACATT
    TCTAAACCTT CCGCAGAAAA TAGCGACAGA TCCATCAAGA CGTCATGCTC TTGGCACACG
    CTCCGCATGA GAAAACGGGA CGGGACGGAG TCCAACGGCC CATGTAAGAG GCAAGAGGGC
    CTGGGCAGCA TGGCGAAAGA CCCCAATAAC AGTTCAGAGG AGTCTGAATG CGATGACCTT
    GACCCCAGTG CTAGTATGGA GATAATGAAC CCTAATTTCA TCCAGGAAGT GGCCCCTGAG
    TTCCTGGTGT CCCTGGTGGA TGTCATGTGT GTTCTTGGGG ACACAGTCAC CCTACAGTGC
    AAAGTGTGCG GCCGGCCGAA GCCCAGTGTC ACCTGGAAGG GACCCGACCA GAGCATCTTG
    GATGGGAACA GCAACACCTA CTCCATGTCA ACCAGTGAGT CTGGAGACAT CAAGCTGAAG
    ATCTGCCACG TGATGCCCCA GGATAGTGGC ATCTACACGT GTGTGGCCAG TAATGAGCTG
    GGGGCAACCT CGACATCTGC CACCATCAAA GTGCAAGGGG TCCCTGGGGC CCCCAGCCAG
    CCGCTGGCTC AGGAGAGAAG CAGCACCTCC GTCATCCTCC GCTGGCTGCC ACCTACCAGC
    ACCGGCAATT GCACTATATC CGGCTACACT GTGGAATACA AGGAGGAGGG TGCCCAACTA
    TGGCAGCAGT CAGTCGCCTC TACCCTGGAT ACTTACCTCG TCCTAGAAGA CCTGTGCCCT
    GGGGCCCGGT ACCAGTTCCG CGTGAGCGCC AGTAACCCCT GGGGTATAAG TCTGCCGAGT
    GAGCCTTCCG AGCCAGTCAG GCTTCCAGAA ACTGATACCA ATTCCAACGG ATCAACAATT
    TCATGGAAAG GAAACTTTGA GCAAGTCTAC ACAGAGCTGC ATGAGATTGG AAGGGGCAGG
    TTCTCCATAG TGAAGAAATG TGCCAAGAAA CAGAGCAGGA AGGACGTGGC GGCCAAATTC
    ATCAGCAAGA AGCTAAAGAA GCGAGAGCAG GCGGCGCACG AGGCGACACT TCTCCAGCAC
    CTACAGCACC CACAGTACAT TACCATCCAT GACACCTTTG AGTCGGACGG TGCCTACATC
    CTCATCCTGG ACCTCATGGA AGATGGCCGC TTGCTGGATT ACCTCATGAA ACACGACGAG
    CTGATGGAAG AGAAAGTGGC GTTCTACATG CGAGACATCA TGGAGGCGCT GCAGTACCTG
    CACAGCTGCC GCGTGGCGCA TTTAGACATC AAGCCGGAGA ATCTCCTCAT AGACCTGCGC
    ATCCCGGTGC CCAGAGTGAA GATCATCGAC CTGGAGGACG CCGTGCAGAT AACGGGGCAC
    CATTATATCC ATCAGCTGCT GGGGAACCCC GAGTTTGCTG CCCCCGAGCT TATCCAAGGG
    AGCCCCGTCT CTCTGGGCAC AGACATCTGG AGCTTGGGGG TGCTGACCTA CGTCATGCTG
    AGCGGGGTCT CCCCGTTCCT GGACGAGAGC AGCGAGGAGA CCTGCGCCAA CATCTGCCGG
    GTGGACTTCA GTTTCCCCCA GGAGTATTTC TCGGGGGTCA GTCAGGCTGC CCAGGACTTC
    ATCAAGGCCA CGTTGCAAGG GGACCCTCGC TGGAGACCCA CCATCGCCAC CTGTCTGCAG
    CACCCATGGC TGCAGCCTCA TAACAACACT TACTCCAAAT TACCCCTGGA CACCTCCCGC
    CTGGCTGGCT TCATTGAGAG GCGGAGCCAT CAAAACGACG CCCAGCCATT GGTCAAAACC
    AAGAGTCTCA TCATTAGCAG GAACGTGGTG GGAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa66026
    Clone ID Related Accession (Same CDS sequence) XM_031892645.1
    Accession Version XM_031892645.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kalirin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAAGGAA CAAAGGATTC ATTTTCTGTC TCCGCGGCGG AAAATGGGGG GAAGTCCGAG 
    TCGGTGGCCA ATCTGCAGCC CCAGGCGTCC CTGAATTCTA TCCACAGCTC CCCGGGCCCC
    AAACGCTCCA CCAACACCCT AAAAAAGTGG CTGACGAGCC CTGTGAGGCG ACTGAACAGC
    GGGAAGGGCG AGAGCAACAT CAAGAAGCAA AAGAAAAGAG ACGGGCGCAA CCTCGGCTCC
    CCCAGAGCTG GGGACGAGAC CACCCCACAG GGAGACAGCG CCGATGAGAA GGGCAAGAAG
    GGGTGGGACG AAGAGGCTGA CGACGAATCC CACACTCCGC TTCCTCCGCC AATGAAGATC
    TTTGATAATG ACCCCACTCC GGACGACAAG TCCTCCCTGC TTGTGGTCCG GCAGAGCTCA
    GCGGAGGTGC CTAGTGCGGC TGAACTGGTC ACTGCCATAG AGAATCTGGT CAGGAACAAG
    ATGACCATTG ACGGAGGTTC TTATCGGGGC GCACAGAACA TAAATGAAGG ATTTGTGAAT
    GAGGGTTTGG CCCCCTCGCC AGCTCCCAGG AACCACGAAG AGGAGCAAAA AGCCAAAGCA
    CTCAGGGGCA GGATGTTTGT ACTGAATGAG CTGGTACAGA CGGAGAAGGA CTATGTCCGG
    GATCTTGGGT TTATGGTAGA GACGTACATC CCCAAGATGG AGGAGAGGGG GACCCCGGAA
    GATATGAACG GAAAAGACAA GATCGTATTC GGCAACATCC ACCAGATCTA CGATTGGCAC
    AAAGACTTTT ACTTGGCAGA ACTAGAGAAA TGCCTATTGG AGCCGGAGAG GTTGGCGCAG
    TTATTTATAA AACATGAGAG GCGGCTGCAC ATGTATGTGG TTTATTGCCA AAACAAACCG
    CGCTCGGAGT TTGTCGTTGC CGAATATGAC GCCTACTTTG AGGATCTCAT GCAGGAGGTG
    AATCCCAGAT TTACTGTCAG TGATTTTCTC ATCAAACCGA TCCAAAGGAT AACTAAGTAC
    CAACTGCTGC TAAAGGACTT CCTTAAGTAC AGTCAGAAGG CGGGTTTGGA GTGTTCAGAA
    ATCGAGAAAG CCGTGGAACT AATGTGCCTT GTCCCGAAAC GCTGCAACGA CATGATGAAC
    CTGGGGCGCC TGCAGGGATT CGAGGGGAAG CTGACAGCCC AGGGGAAGCT GTTGCAGCAG
    GACACCTTCT ATGTGATCGA GCAGGAATCG GGGGTCCAGT CTCGGACGAA GGAAAGGCGG
    GTTTTCCTGT TCGAGCAGAT TGTTATTTTC AGCGAGCTCC TGCGGAAAGG TTCCTCCACG
    CCGGGGTACA TGTTCAAAAG GGGCATAAAG ATGAATTATC TCATCTTAGA AGAGAACATT
    GACAACGACC CGTGCAAATT CGCCATTCGG AGCCGCGAGA CGTCGGAGCG GGTGATCCTG
    CAAGCGGCCA ACGCCGACAT TAAACAGGCC TGGGTGCAGG ACATCGGGCA AGTGCTGGAG
    ACCCAGAGGG ACTTCCTAAA CGCCCTGCAA TCGCCAATAG AATACCAGCG GAAGGAGAGC
    AACGCAGCCA TGAACAAGCC TCAGATTGGC CGCGGGCCGT CCCCCACCGT TAGGCCTCTC
    TCCTCTACGC CCGTAGGGTG TGAAAAAGAT GTCACCATCC CAGTGCGGAA CCAGTCGCTA
    CCAGCGCGGA AAATACCTAC CTCTAACGGC AGCGCCGGGT ATGAAATGTA CCCACGGGCG
    GAGAAATGTG ACCCAAACCA GAGCGAGCTG AGCGGCTGCA ATGGGACCAA CTCCATGGTG
    GTTGTACAGG ATTACTATGC ACTAAAGGAG AATGAGATCT GCGTGTACCA CGGGGAGTTG
    GTACAGATTC TGGCCATCAA TCAGCAGAAC ATGTTCCTGG TATTCCGGCC GTCCAATGAC
    CATTCCCCGG CAGCGGAAGG CTGGATCCCG GGATCAGTTT TGGGCCACAT TTCTAAACCT
    TCCGCAGAAA ATAGCGACAG ATCCATCAAG ACGTCATGCT CTTGGCACAC GCTCCGCATG
    AGAAAACGGG ACGGGACGGA GTCCAACGGC CCATGTAAGA GGCAAGAGGG CCTGGGCAGC
    ATGGCGAAAG ACCCCAATAA CAGTTCAGAG GAGTCTGAAT GCGATGACCT TGACCCCAGT
    GCTAGTATGG AGATAATGAA CCCTAATTTC ATCCAGGAAG TGGCCCCTGA GTTCCTGGTG
    TCCCTGGTGG ATGTCATGTG TGTTCTTGGG GACACAGTCA CCCTACAGTG CAAAGTGTGC
    GGCCGGCCGA AGCCCAGTGT CACCTGGAAG GGACCCGACC AGAGCATCTT GGATGGGAAC
    AGCAACACCT ACTCCATGTC AACCAGTGAG TCTGGAGACA TCAAGCTGAA GATCTGCCAC
    GTGATGCCCC AGGATAGTGG CATCTACACG TGTGTGGCCA GTAATGAGCT GGGGGCAACC
    TCGACATCTG CCACCATCAA AGTGCAAGGG GTCCCTGGGG CCCCCAGCCA GCCGCTGGCT
    CAGGAGAGAA GCAGCACCTC CGTCATCCTC CGCTGGCTGC CACCTACCAG CACCGGCAAT
    TGCACTATAT CCGGCTACAC TGTGGAATAC AAGGAGGAGG GTGCCCAACT ATGGCAGCAG
    TCAGTCGCCT CTACCCTGGA TACTTACCTC GTCCTAGAAG ACCTGTGCCC TGGGGCCCGG
    TACCAGTTCC GCGTGAGCGC CAGTAACCCC TGGGGTATAA GTCTGCCGAG TGAGCCTTCC
    GAGCCAGTCA GGCTTCCAGA AACTGATACC AATTCCAACG GATCAACAAT TTCATGGAAA
    GGAAACTTTG AGCAAGTCTA CACAGAGCTG CATGAGATTG GAAGGGGCAG GTTCTCCATA
    GTGAAGAAAT GTGCCAAGAA ACAGAGCAGG AAGGACGTGG CGGCCAAATT CATCAGCAAG
    AAGCTAAAGA AGCGAGAGCA GGCGGCGCAC GAGGCGACAC TTCTCCAGCA CCTACAGCAC
    CCACAGTACA TTACCATCCA TGACACCTTT GAGTCGGACG GTGCCTACAT CCTCATCCTG
    GACCTCATGG AAGATGGCCG CTTGCTGGAT TACCTCATGA AACACGACGA GCTGATGGAA
    GAGAAAGTGG CGTTCTACAT GCGAGACATC ATGGAGGCGC TGCAGTACCT GCACAGCTGC
    CGCGTGGCGC ATTTAGACAT CAAGCCGGAG AATCTCCTCA TAGACCTGCG CATCCCGGTG
    CCCAGAGTGA AGATCATCGA CCTGGAGGAC GCCGTGCAGA TAACGGGGCA CCATTATATC
    CATCAGCTGC TGGGGAACCC CGAGTTTGCT GCCCCCGAGC TTATCCAAGG GAGCCCCGTC
    TCTCTGGGCA CAGACATCTG GAGCTTGGGG GTGCTGACCT ACGTCATGCT GAGCGGGGTC
    TCCCCGTTCC TGGACGAGAG CAGCGAGGAG ACCTGCGCCA ACATCTGCCG GGTGGACTTC
    AGTTTCCCCC AGGAGTATTT CTCGGGGGTC AGTCAGGCTG CCCAGGACTT CATCAAGGCC
    ACGTTGCAAG GGGACCCTCG CTGGAGACCC ACCATCGCCA CCTGTCTGCA GCACCCATGG
    CTGCAGCCTC ATAACAACAC TTACTCCAAA TTACCCCTGG ACACCTCCCG CCTGGCTGGC
    TTCATTGAGA GGCGGAGCCA TCAAAACGAC GCCCAGCCAT TGGTCAAAAC CAAGAGTCTC
    ATCATTAGCA GGAACGTGGT GGGAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748505.1
    CDS134..3883
    Translation

    Target ORF information:

    RefSeq Version XM_031892645.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kalirin RhoGEF kinase (kalrn), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892645.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAAGGAA CAAAGGATTC ATTTTCTGTC TCCGCGGCGG AAAATGGGGG GAAGTCCGAG 
    TCGGTGGCCA ATCTGCAGCC CCAGGCGTCC CTGAATTCTA TCCACAGCTC CCCGGGCCCC
    AAACGCTCCA CCAACACCCT AAAAAAGTGG CTGACGAGCC CTGTGAGGCG ACTGAACAGC
    GGGAAGGGCG AGAGCAACAT CAAGAAGCAA AAGAAAAGAG ACGGGCGCAA CCTCGGCTCC
    CCCAGAGCTG GGGACGAGAC CACCCCACAG GGAGACAGCG CCGATGAGAA GGGCAAGAAG
    GGGTGGGACG AAGAGGCTGA CGACGAATCC CACACTCCGC TTCCTCCGCC AATGAAGATC
    TTTGATAATG ACCCCACTCC GGACGACAAG TCCTCCCTGC TTGTGGTCCG GCAGAGCTCA
    GCGGAGGTGC CTAGTGCGGC TGAACTGGTC ACTGCCATAG AGAATCTGGT CAGGAACAAG
    ATGACCATTG ACGGAGGTTC TTATCGGGGC GCACAGAACA TAAATGAAGG ATTTGTGAAT
    GAGGGTTTGG CCCCCTCGCC AGCTCCCAGG AACCACGAAG AGGAGCAAAA AGCCAAAGCA
    CTCAGGGGCA GGATGTTTGT ACTGAATGAG CTGGTACAGA CGGAGAAGGA CTATGTCCGG
    GATCTTGGGT TTATGGTAGA GACGTACATC CCCAAGATGG AGGAGAGGGG GACCCCGGAA
    GATATGAACG GAAAAGACAA GATCGTATTC GGCAACATCC ACCAGATCTA CGATTGGCAC
    AAAGACTTTT ACTTGGCAGA ACTAGAGAAA TGCCTATTGG AGCCGGAGAG GTTGGCGCAG
    TTATTTATAA AACATGAGAG GCGGCTGCAC ATGTATGTGG TTTATTGCCA AAACAAACCG
    CGCTCGGAGT TTGTCGTTGC CGAATATGAC GCCTACTTTG AGGATCTCAT GCAGGAGGTG
    AATCCCAGAT TTACTGTCAG TGATTTTCTC ATCAAACCGA TCCAAAGGAT AACTAAGTAC
    CAACTGCTGC TAAAGGACTT CCTTAAGTAC AGTCAGAAGG CGGGTTTGGA GTGTTCAGAA
    ATCGAGAAAG CCGTGGAACT AATGTGCCTT GTCCCGAAAC GCTGCAACGA CATGATGAAC
    CTGGGGCGCC TGCAGGGATT CGAGGGGAAG CTGACAGCCC AGGGGAAGCT GTTGCAGCAG
    GACACCTTCT ATGTGATCGA GCAGGAATCG GGGGTCCAGT CTCGGACGAA GGAAAGGCGG
    GTTTTCCTGT TCGAGCAGAT TGTTATTTTC AGCGAGCTCC TGCGGAAAGG TTCCTCCACG
    CCGGGGTACA TGTTCAAAAG GGGCATAAAG ATGAATTATC TCATCTTAGA AGAGAACATT
    GACAACGACC CGTGCAAATT CGCCATTCGG AGCCGCGAGA CGTCGGAGCG GGTGATCCTG
    CAAGCGGCCA ACGCCGACAT TAAACAGGCC TGGGTGCAGG ACATCGGGCA AGTGCTGGAG
    ACCCAGAGGG ACTTCCTAAA CGCCCTGCAA TCGCCAATAG AATACCAGCG GAAGGAGAGC
    AACGCAGCCA TGAACAAGCC TCAGATTGGC CGCGGGCCGT CCCCCACCGT TAGGCCTCTC
    TCCTCTACGC CCGTAGGGTG TGAAAAAGAT GTCACCATCC CAGTGCGGAA CCAGTCGCTA
    CCAGCGCGGA AAATACCTAC CTCTAACGGC AGCGCCGGGT ATGAAATGTA CCCACGGGCG
    GAGAAATGTG ACCCAAACCA GAGCGAGCTG AGCGGCTGCA ATGGGACCAA CTCCATGGTG
    GTTGTACAGG ATTACTATGC ACTAAAGGAG AATGAGATCT GCGTGTACCA CGGGGAGTTG
    GTACAGATTC TGGCCATCAA TCAGCAGAAC ATGTTCCTGG TATTCCGGCC GTCCAATGAC
    CATTCCCCGG CAGCGGAAGG CTGGATCCCG GGATCAGTTT TGGGCCACAT TTCTAAACCT
    TCCGCAGAAA ATAGCGACAG ATCCATCAAG ACGTCATGCT CTTGGCACAC GCTCCGCATG
    AGAAAACGGG ACGGGACGGA GTCCAACGGC CCATGTAAGA GGCAAGAGGG CCTGGGCAGC
    ATGGCGAAAG ACCCCAATAA CAGTTCAGAG GAGTCTGAAT GCGATGACCT TGACCCCAGT
    GCTAGTATGG AGATAATGAA CCCTAATTTC ATCCAGGAAG TGGCCCCTGA GTTCCTGGTG
    TCCCTGGTGG ATGTCATGTG TGTTCTTGGG GACACAGTCA CCCTACAGTG CAAAGTGTGC
    GGCCGGCCGA AGCCCAGTGT CACCTGGAAG GGACCCGACC AGAGCATCTT GGATGGGAAC
    AGCAACACCT ACTCCATGTC AACCAGTGAG TCTGGAGACA TCAAGCTGAA GATCTGCCAC
    GTGATGCCCC AGGATAGTGG CATCTACACG TGTGTGGCCA GTAATGAGCT GGGGGCAACC
    TCGACATCTG CCACCATCAA AGTGCAAGGG GTCCCTGGGG CCCCCAGCCA GCCGCTGGCT
    CAGGAGAGAA GCAGCACCTC CGTCATCCTC CGCTGGCTGC CACCTACCAG CACCGGCAAT
    TGCACTATAT CCGGCTACAC TGTGGAATAC AAGGAGGAGG GTGCCCAACT ATGGCAGCAG
    TCAGTCGCCT CTACCCTGGA TACTTACCTC GTCCTAGAAG ACCTGTGCCC TGGGGCCCGG
    TACCAGTTCC GCGTGAGCGC CAGTAACCCC TGGGGTATAA GTCTGCCGAG TGAGCCTTCC
    GAGCCAGTCA GGCTTCCAGA AACTGATACC AATTCCAACG GATCAACAAT TTCATGGAAA
    GGAAACTTTG AGCAAGTCTA CACAGAGCTG CATGAGATTG GAAGGGGCAG GTTCTCCATA
    GTGAAGAAAT GTGCCAAGAA ACAGAGCAGG AAGGACGTGG CGGCCAAATT CATCAGCAAG
    AAGCTAAAGA AGCGAGAGCA GGCGGCGCAC GAGGCGACAC TTCTCCAGCA CCTACAGCAC
    CCACAGTACA TTACCATCCA TGACACCTTT GAGTCGGACG GTGCCTACAT CCTCATCCTG
    GACCTCATGG AAGATGGCCG CTTGCTGGAT TACCTCATGA AACACGACGA GCTGATGGAA
    GAGAAAGTGG CGTTCTACAT GCGAGACATC ATGGAGGCGC TGCAGTACCT GCACAGCTGC
    CGCGTGGCGC ATTTAGACAT CAAGCCGGAG AATCTCCTCA TAGACCTGCG CATCCCGGTG
    CCCAGAGTGA AGATCATCGA CCTGGAGGAC GCCGTGCAGA TAACGGGGCA CCATTATATC
    CATCAGCTGC TGGGGAACCC CGAGTTTGCT GCCCCCGAGC TTATCCAAGG GAGCCCCGTC
    TCTCTGGGCA CAGACATCTG GAGCTTGGGG GTGCTGACCT ACGTCATGCT GAGCGGGGTC
    TCCCCGTTCC TGGACGAGAG CAGCGAGGAG ACCTGCGCCA ACATCTGCCG GGTGGACTTC
    AGTTTCCCCC AGGAGTATTT CTCGGGGGTC AGTCAGGCTG CCCAGGACTT CATCAAGGCC
    ACGTTGCAAG GGGACCCTCG CTGGAGACCC ACCATCGCCA CCTGTCTGCA GCACCCATGG
    CTGCAGCCTC ATAACAACAC TTACTCCAAA TTACCCCTGG ACACCTCCCG CCTGGCTGGC
    TTCATTGAGA GGCGGAGCCA TCAAAACGAC GCCCAGCCAT TGGTCAAAAC CAAGAGTCTC
    ATCATTAGCA GGAACGTGGT GGGAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa27528
    Clone ID Related Accession (Same CDS sequence) XM_012970399.2
    Accession Version XM_012970399.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kalirin, RhoGEF kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683527.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970399.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAAGGGTG GCACTGCCAA GTACCCCTCG GGCTCCTGCC TGGGGTGGCT CTGTACCCGC 
    TGCTGCTGCT GCTTCTTTGC AACAGATTCA TTTTCTGTCT CCGCGGCGGA AAATGGGGGG
    AAGTCCGAGT CGGTGGCCAA TCTGCAGCCC CAGGCGTCCC TGAATTCTAT CCACAGCTCC
    CCGGGCCCCA AACGCTCCAC CAACACCCTA AAAAAGTGGC TGACGAGCCC TGTGAGGCGA
    CTGAACAGCG GGAAGGGCGA GAGCAACATC AAGAAGCAAA AGAAAAGAGA CGGGCGCAAC
    CTCGGCTCCC CCAGAGCTGG GGACGAGACC ACCCCACAGG GAGACAGCGC CGATGAGAAG
    GGCAAGAAGG GGTGGGACGA AGAGGCTGAC GACGAATCCC ACACTCCGCT TCCTCCGCCA
    ATGAAGATCT TTGATAATGA CCCCACTCCG GACGACAAGT CCTCCCTGCT TGTGGTCCGG
    CAGAGCTCAG CGGAGGTGCC TAGTGCGGCT GAACTGGTCA CTGCCATAGA GAATCTGGTC
    AGGAACAAGA TGACCATTGA CGGAGGTTCT TATCGGGGCG CACAGAACAT AAATGAAGGA
    TTTGTGAATG AGGGTTTGGC CCCCTCGCCA GCTCCCAGGA ACCACGAAGA GGAGCAAAAA
    GCCAAAGCAC TCAGGGGCAG GATGTTTGTA CTGAATGAGC TGGTACAGAC GGAGAAGGAC
    TATGTCCGGG ATCTTGGGTT TATGGTAGAG ACGTACATCC CCAAGATGGA GGAGAGGGGG
    ACCCCGGAAG ATATGAACGG AAAAGACAAG ATCGTATTCG GCAACATCCA CCAGATCTAC
    GATTGGCACA AAGACTTTTA CTTGGCAGAA CTAGAGAAAT GCCTATTGGA GCCGGAGAGG
    TTGGCGCAGT TATTTATAAA ACATGAGAGG CGGCTGCACA TGTATGTGGT TTATTGCCAA
    AACAAACCGC GCTCGGAGTT TGTCGTTGCC GAATATGACG CCTACTTTGA GGATCTCATG
    CAGGAGGTGA ATCCCAGATT TACTGTCAGT GATTTTCTCA TCAAACCGAT CCAAAGGATA
    ACTAAGTACC AACTGCTGCT AAAGGACTTC CTTAAGTACA GTCAGAAGGC GGGTTTGGAG
    TGTTCAGAAA TCGAGAAAGC CGTGGAACTA ATGTGCCTTG TCCCGAAACG CTGCAACGAC
    ATGATGAACC TGGGGCGCCT GCAGGGATTC GAGGGGAAGC TGACAGCCCA GGGGAAGCTG
    TTGCAGCAGG ACACCTTCTA TGTGATCGAG CAGGAATCGG GGGTCCAGTC TCGGACGAAG
    GAAAGGCGGG TTTTCCTGTT CGAGCAGATT GTTATTTTCA GCGAGCTCCT GCGGAAAGGT
    TCCTCCACGC CGGGGTACAT GTTCAAAAGG GGCATAAAGA TGAATTATCT CATCTTAGAA
    GAGAACATTG ACAACGACCC GTGCAAATTC GCCATTCGGA GCCGCGAGAC GTCGGAGCGG
    GTGATCCTGC AAGCGGCCAA CGCCGACATT AAACAGGCCT GGGTGCAGGA CATCGGGCAA
    GTGCTGGAGA CCCAGAGGGA CTTCCTAAAC GCCCTGCAAT CGCCAATAGA ATACCAGCGG
    AAGGAGAGCA ACGCAGCCAT GAACAAGCCT CAGATTGGCC GCGGGCCGTC CCCCACCGTT
    AGGCCTCTCT CCTCTACGCC CGTAGGGTGT GAAAAAGATG TCACCATCCC AGTGCGGAAC
    CAGTCGCTAC CAGCGCGGAA AATACCTACC TCTAACGGCA GCGCCGGGTA TGAAATGTAC
    CCACGGGCGG AGAAATGTGA CCCAAACCAG AGCGAGCTGA GCGGCTGCAA TGGGACCAAC
    TCCATGGTGG TTGTACAGGA TTACTATGCA CTAAAGGAGA ATGAGATCTG CGTGTACCAC
    GGGGAGTTGG TACAGATTCT GGCCATCAAT CAGCAGAACA TGTTCCTGGT ATTCCGGCCG
    TCCAATGACC ATTCCCCGGC AGCGGAAGGC TGGATCCCGG GATCAGTTTT GGGCCACATT
    TCTAAACCTT CCGCAGAAAA TAGCGACAGA TCCATCAAGA CGTCATGCTC TTGGCACACG
    CTCCGCATGA GAAAACGGGA CGGGACGGAG TCCAACGGCC CATGTAAGAG GCAAGAGGGC
    CTGGGCAGCA TGGCGAAAGA CCCCAATAAC AGTTCAGAGG AGTCTGAATG CGATGACCTT
    GACCCCAGTG CTAGTATGGA GGTAAGGAAG CGTGGGGACA CAGTCATCCT ACAGTGCAAA
    GTGTCCGGCC GCCCGAAGCC CAGTGTCACC TGGAAGGGAC CCGACCAGAG CATCTTGGAT
    GGGAACAGCA ACACCTACTC CATGTCAACC AGTGAGTCTG GAGACATCAA GCTGAAGATC
    TGCCACGTGA TGCCCCAGGA TAGTGGCATC TACACGTGTG TGGCCAGTAA TGAGCTGGGG
    GCAACCTCGA CATCTGCCAC CATCAAAGTG CAAGGGGTCC CTGGGGCCCC CAGCCAGCCG
    CTGGCTCAGG AGAGAAGCAG CACCTCCGTC ATCCTCCGCT GGCTGCCACC TACCAGCACC
    GGCAATTGCA CTATATCCGG CTACACTGTG GAATACAAGG AGGAGGGTGC CCAACTATGG
    CAGCAGTCAG TCGCCTCTAC CCTGGATACT TACCTCGTCC TAGAAGACCT GTGCCCTGGG
    GCCCGGTACC AGTTCCGCGT GAGCGCCAGT AACCCCTGGG GTATAAGTCT GCCGAGTGAG
    CCTTCCGAGC CAGTCAGGCT TCCAGAAACT GATACCAATT CCAACGGATC AACGATTTCA
    TGGAAAGGAA ACTTTGAGCA AGTCTACACA GAGCTGCATG AGATTGGAAG GGGCAGGTTC
    TCCATAGTGA AGAAATGTGC CAAGAAACAG AGCAGGAAGG ACGTGGCGGC CAAATTCATC
    AGCAAGAAGC TAAAGAAGCG AGAGCAGGCG GCGCACGAGG CGACACTTCT CCAGCACCTA
    CAGCACCCAC AGTACATTAC CATCCATGAC ACCTTTGAGT CGGACGGTGC CTACATCCTC
    ATCCTGGACC TCATGGAAGA TGGCCGCTTG CTGGATTACC TCATGAAACA CGACGAGCTG
    ATGGAAGAGA AAGTGGCGTT CTACATGCGA GACATCATGG AGGCGCTGCA GTACCTGCAC
    AGCTGCCGCG TGGCGCATTT AGACATCAAG CCGGAGAATC TCCTCATAGA CCTGCGCATC
    CCGGTGCCCA GAGTGAAGAT CATCGACCTG GAGGACGCCG TGCAGATAAC GGGGCACCAT
    TATATCCATC AGCTGCTGGG GAACCCCGAG TTTGCTGCCC CCGAGCTTAT CCAAGGGAGC
    CCCGTCTCTC TGGGCACAGA CATCTGGAGC TTGGGGGTGC TGACCTACGT CATGCTGAGC
    GGGGTCTCCC CGTTCCTGGA CGAGAGCAGC GAGGAGACCT GCGCCAACAT CTGCCGGGTG
    GACTTCAGTT TCCCCCAGGA GTATTTCTCG GGGGTCAGTC AGGCTGCCCA GGACTTCATC
    AAGGCCACGT TGCAAGGGGA CCCTCGCTGG AGACCCACCA TCGCCACCTG TCTGCAGCAC
    CCATGGCTGC AGCCTCATAA CAACACTTAC TCCAAATTAC CCCTGGACAC CTCCCGCCTG
    GCTGGCTTCA TTGAGAGGCG GAGCCATCAA AACGACGCCC AGCCATTGGT CAAAACCAAG
    AGTCTCATCA TTAGCAGGAA CGTGGTGGGA ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825853.1
    CDS154..3909
    Translation

    Target ORF information:

    RefSeq Version XM_012970399.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kalirin, RhoGEF kinase (kalrn), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970399.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAAGGGTG GCACTGCCAA GTACCCCTCG GGCTCCTGCC TGGGGTGGCT CTGTACCCGC 
    TGCTGCTGCT GCTTCTTTGC AACAGATTCA TTTTCTGTCT CCGCGGCGGA AAATGGGGGG
    AAGTCCGAGT CGGTGGCCAA TCTGCAGCCC CAGGCGTCCC TGAATTCTAT CCACAGCTCC
    CCGGGCCCCA AACGCTCCAC CAACACCCTA AAAAAGTGGC TGACGAGCCC TGTGAGGCGA
    CTGAACAGCG GGAAGGGCGA GAGCAACATC AAGAAGCAAA AGAAAAGAGA CGGGCGCAAC
    CTCGGCTCCC CCAGAGCTGG GGACGAGACC ACCCCACAGG GAGACAGCGC CGATGAGAAG
    GGCAAGAAGG GGTGGGACGA AGAGGCTGAC GACGAATCCC ACACTCCGCT TCCTCCGCCA
    ATGAAGATCT TTGATAATGA CCCCACTCCG GACGACAAGT CCTCCCTGCT TGTGGTCCGG
    CAGAGCTCAG CGGAGGTGCC TAGTGCGGCT GAACTGGTCA CTGCCATAGA GAATCTGGTC
    AGGAACAAGA TGACCATTGA CGGAGGTTCT TATCGGGGCG CACAGAACAT AAATGAAGGA
    TTTGTGAATG AGGGTTTGGC CCCCTCGCCA GCTCCCAGGA ACCACGAAGA GGAGCAAAAA
    GCCAAAGCAC TCAGGGGCAG GATGTTTGTA CTGAATGAGC TGGTACAGAC GGAGAAGGAC
    TATGTCCGGG ATCTTGGGTT TATGGTAGAG ACGTACATCC CCAAGATGGA GGAGAGGGGG
    ACCCCGGAAG ATATGAACGG AAAAGACAAG ATCGTATTCG GCAACATCCA CCAGATCTAC
    GATTGGCACA AAGACTTTTA CTTGGCAGAA CTAGAGAAAT GCCTATTGGA GCCGGAGAGG
    TTGGCGCAGT TATTTATAAA ACATGAGAGG CGGCTGCACA TGTATGTGGT TTATTGCCAA
    AACAAACCGC GCTCGGAGTT TGTCGTTGCC GAATATGACG CCTACTTTGA GGATCTCATG
    CAGGAGGTGA ATCCCAGATT TACTGTCAGT GATTTTCTCA TCAAACCGAT CCAAAGGATA
    ACTAAGTACC AACTGCTGCT AAAGGACTTC CTTAAGTACA GTCAGAAGGC GGGTTTGGAG
    TGTTCAGAAA TCGAGAAAGC CGTGGAACTA ATGTGCCTTG TCCCGAAACG CTGCAACGAC
    ATGATGAACC TGGGGCGCCT GCAGGGATTC GAGGGGAAGC TGACAGCCCA GGGGAAGCTG
    TTGCAGCAGG ACACCTTCTA TGTGATCGAG CAGGAATCGG GGGTCCAGTC TCGGACGAAG
    GAAAGGCGGG TTTTCCTGTT CGAGCAGATT GTTATTTTCA GCGAGCTCCT GCGGAAAGGT
    TCCTCCACGC CGGGGTACAT GTTCAAAAGG GGCATAAAGA TGAATTATCT CATCTTAGAA
    GAGAACATTG ACAACGACCC GTGCAAATTC GCCATTCGGA GCCGCGAGAC GTCGGAGCGG
    GTGATCCTGC AAGCGGCCAA CGCCGACATT AAACAGGCCT GGGTGCAGGA CATCGGGCAA
    GTGCTGGAGA CCCAGAGGGA CTTCCTAAAC GCCCTGCAAT CGCCAATAGA ATACCAGCGG
    AAGGAGAGCA ACGCAGCCAT GAACAAGCCT CAGATTGGCC GCGGGCCGTC CCCCACCGTT
    AGGCCTCTCT CCTCTACGCC CGTAGGGTGT GAAAAAGATG TCACCATCCC AGTGCGGAAC
    CAGTCGCTAC CAGCGCGGAA AATACCTACC TCTAACGGCA GCGCCGGGTA TGAAATGTAC
    CCACGGGCGG AGAAATGTGA CCCAAACCAG AGCGAGCTGA GCGGCTGCAA TGGGACCAAC
    TCCATGGTGG TTGTACAGGA TTACTATGCA CTAAAGGAGA ATGAGATCTG CGTGTACCAC
    GGGGAGTTGG TACAGATTCT GGCCATCAAT CAGCAGAACA TGTTCCTGGT ATTCCGGCCG
    TCCAATGACC ATTCCCCGGC AGCGGAAGGC TGGATCCCGG GATCAGTTTT GGGCCACATT
    TCTAAACCTT CCGCAGAAAA TAGCGACAGA TCCATCAAGA CGTCATGCTC TTGGCACACG
    CTCCGCATGA GAAAACGGGA CGGGACGGAG TCCAACGGCC CATGTAAGAG GCAAGAGGGC
    CTGGGCAGCA TGGCGAAAGA CCCCAATAAC AGTTCAGAGG AGTCTGAATG CGATGACCTT
    GACCCCAGTG CTAGTATGGA GGTAAGGAAG CGTGGGGACA CAGTCATCCT ACAGTGCAAA
    GTGTCCGGCC GCCCGAAGCC CAGTGTCACC TGGAAGGGAC CCGACCAGAG CATCTTGGAT
    GGGAACAGCA ACACCTACTC CATGTCAACC AGTGAGTCTG GAGACATCAA GCTGAAGATC
    TGCCACGTGA TGCCCCAGGA TAGTGGCATC TACACGTGTG TGGCCAGTAA TGAGCTGGGG
    GCAACCTCGA CATCTGCCAC CATCAAAGTG CAAGGGGTCC CTGGGGCCCC CAGCCAGCCG
    CTGGCTCAGG AGAGAAGCAG CACCTCCGTC ATCCTCCGCT GGCTGCCACC TACCAGCACC
    GGCAATTGCA CTATATCCGG CTACACTGTG GAATACAAGG AGGAGGGTGC CCAACTATGG
    CAGCAGTCAG TCGCCTCTAC CCTGGATACT TACCTCGTCC TAGAAGACCT GTGCCCTGGG
    GCCCGGTACC AGTTCCGCGT GAGCGCCAGT AACCCCTGGG GTATAAGTCT GCCGAGTGAG
    CCTTCCGAGC CAGTCAGGCT TCCAGAAACT GATACCAATT CCAACGGATC AACGATTTCA
    TGGAAAGGAA ACTTTGAGCA AGTCTACACA GAGCTGCATG AGATTGGAAG GGGCAGGTTC
    TCCATAGTGA AGAAATGTGC CAAGAAACAG AGCAGGAAGG ACGTGGCGGC CAAATTCATC
    AGCAAGAAGC TAAAGAAGCG AGAGCAGGCG GCGCACGAGG CGACACTTCT CCAGCACCTA
    CAGCACCCAC AGTACATTAC CATCCATGAC ACCTTTGAGT CGGACGGTGC CTACATCCTC
    ATCCTGGACC TCATGGAAGA TGGCCGCTTG CTGGATTACC TCATGAAACA CGACGAGCTG
    ATGGAAGAGA AAGTGGCGTT CTACATGCGA GACATCATGG AGGCGCTGCA GTACCTGCAC
    AGCTGCCGCG TGGCGCATTT AGACATCAAG CCGGAGAATC TCCTCATAGA CCTGCGCATC
    CCGGTGCCCA GAGTGAAGAT CATCGACCTG GAGGACGCCG TGCAGATAAC GGGGCACCAT
    TATATCCATC AGCTGCTGGG GAACCCCGAG TTTGCTGCCC CCGAGCTTAT CCAAGGGAGC
    CCCGTCTCTC TGGGCACAGA CATCTGGAGC TTGGGGGTGC TGACCTACGT CATGCTGAGC
    GGGGTCTCCC CGTTCCTGGA CGAGAGCAGC GAGGAGACCT GCGCCAACAT CTGCCGGGTG
    GACTTCAGTT TCCCCCAGGA GTATTTCTCG GGGGTCAGTC AGGCTGCCCA GGACTTCATC
    AAGGCCACGT TGCAAGGGGA CCCTCGCTGG AGACCCACCA TCGCCACCTG TCTGCAGCAC
    CCATGGCTGC AGCCTCATAA CAACACTTAC TCCAAATTAC CCCTGGACAC CTCCCGCCTG
    GCTGGCTTCA TTGAGAGGCG GAGCCATCAA AACGACGCCC AGCCATTGGT CAAAACCAAG
    AGTCTCATCA TTAGCAGGAA CGTGGTGGGA ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa66017
    Clone ID Related Accession (Same CDS sequence) XM_031892635.1
    Accession Version XM_031892635.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6189bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kalirin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGGCGAGT CGATGCTGAA TGCCAGTCTG GTTAATGCCA GTTCACTGTC GGAAGCTGAG 
    CAGTTGCAGC GAGAACACGA GCAGTTTCAG ATGGCTATTG AGTCCCTTTT CCACGCCAGC
    TCTCTGCAGA AGACTCACCA AAGTGCCCTG CAGGTCCAGC AGAAAGCAGA GGCGCTTCTA
    CAGGCCGGGC ACTACGACGC CGACGCCATC CGGGAATGCG CCGAGAAGGT GGCGATGCAC
    TGGCAGCAAC TCATGCTCAA GATGGAGGAC AGACTCAAGC TAGTGAATGC CTCTGTGGCC
    TTCTATAAAA CCTCCGAGCA GGTGTGCAGC GTTCTGGAGA GCCTGGAGCA GGAGTACCGC
    AGGGATGAGG ACTGGTGTCG GGGCCACGAT AAGCTGGGAC CCTCCACTGA GTCGGACCAC
    GTGATGCCGC TGATCAGTAA ACACCTGGAG CAGAAGGAGG CTTTCCTTAA GGCTTGTACG
    TTAGCGCGGA GGAACGCGGA AGTCTTTCTG AAGTACATTC ACCGTAACAA CGTGAGCATG
    CCCGGCGTGG CCCCCCACAC GCGCGTCCCC GAGCAGCAAG TTAAAGCCAT ACTGAGCGAG
    CTCCTGCAAA GGGAGAACCG GGTGCTGCAC TTTTGGACCC TGAAGAAGCG GCGGTTGGAC
    CAGTGCCAGC AGTACATTGT GTTTGAGAGG AGCGCCAAGC AGGCTCTGGA CTGGATTCAG
    GAAGCCGGGG AGCATTATCT CTCCACTCGC ATCTCCCCTG GGGAATGTAT TGAGGAGACT
    CAGGAGCTGC TGAAGGAATA CGAGGAATTC CGGATACCTG CCAAGCAAAC GAAGGAGAAG
    GTGCGGCTGC TGATCCAGCT GGCAGACAGC TTTGTGGAGA AGGGCCACGT TCACGCCACC
    GAGCTGAAGA AATGGGTCAC TACTGTCAAC AAACACTACC GGGAATTCGC TCTGCGTATG
    GGCAAATACC ATTGCGGCCT GCAGCGAGCC CTGGGCATCT GCACTGAGGA CACAAAGGAC
    CTGGAGTTGG ACATTGTCCC TGCGATTCTG ACTGACCCGG AGGTGAAGCT GCGCGACGCC
    GGCCATGAAC TCAACGAGGA GAAGAGAAAA TCCGCCAGGA AAAAAGAATT CATCATGGCG
    GAACTTCTAC AGACAGAGAA AGCGTATGTG CGGGACTTGC ACGAGTGTAT GGAGACCTAC
    CTGTGGGAAA TGACCAGCGG AGTGGAGGAA ATTCCGCCCG GAATAGTGAA TAAGGAGCAC
    ATCATCTTTG GGAACATTCA GGAAATATAC GACTTTCACA ATAACATCTT CCTGAAAGAG
    CTGGAGAAGT ACGAGCAGCT GCCGGAGGAC GTGGGACATT GTTTTGTCAC TTGGGCCGAC
    AAGTTCCACA TGTACGTGAC CTACTGTAAG AACAAACCCG ACTCCAGTCA GCTGATCCTG
    GAGCACGCCG GCTCCTTCTT TGACGAGATC CAGCAGCGAC ACGGCCTGGC CAACTCCATC
    TCCTCCTACC TCATCAAACC CGTCCAGCGG ATCACTAAGT ACCAGCTGCT TCTCAAGGAG
    CTTCTGACCT GCTGTGAGGA AGGGAAAGGG GAGATAAAGG ACGGGCTGGA GGTGATGCTG
    AGTGTGCCCA AGCGGGCCAA TGATGCCATG CACGTCAGCA TGCTGGAAGG ATTTGACGAA
    AACCTGGACG TTCAAGGGGA ACTTATCCTG CAAGATTCCT TCCAAGTCTG GGACCCCAAA
    TCCCTCATCC GCAAGGGGCG CGACCGGCAC CTCTTCCTCT TCGAAATCTC CCTCGTCTTC
    AGCAAAGAGA TCAAAGACTC CACCGGCCAC ACCAAATACG TCTTTAAGAA CAAACTGCTG
    ACCTCGGAAC TGGGGGTGAC GGAGCACGTA GAGGGGGACC CCTGTAAGTT TGCCCTATGG
    TCGGGGCGCA CCCCATCATC CGATAACAAG ACAGTGCTAA AGGCATCTAG TATCGAGGTG
    AAGCAGGAAT GGATCAAGAA CATCCGGGAA GTCATTCAGG AAAGGATGAT CCATTTGAAA
    GGAGCCTTGA AAGAACCCAT CCAGCCCCCC AAAACTCCAG CAAAGCAGCG CAACAACAGC
    AAAAGGGACG TACTGGAGGA CGCCGATAGC CAGGGGGACG GCAGCAGCCA ACCGGACACC
    ATCTCCATCG CCTCCAGAAC CTCCCAGAAC ACCATGGACA GCGACAAGTT ATCTGGGGGC
    TGTGAACTGA CGACGGTTCT GCAGGATTTC ATCGCTCTCC ACAACAATGA GCTGAGCATT
    CAGGTGGGGC AAACCGTGGA GCTGCTGGAG AGGCCGAGCG ACCGGCCTGG ATGGTGCTTA
    GTCCGGACCA CCGACCGGAG CCCCCCGCAG GAAGGGCTGG TACCCAGCAG CGCCCTGTGC
    ATCTCCCACT CGCGGAGCAG CGTCGAAATG GAGTGTTTCT TCCCTTCAGG CAAAGATTCA
    TTTTCTGTCT CCGCGGCGGA AAATGGGGGG AAGTCCGAGT CGGTGGCCAA TCTGCAGCCC
    CAGGCGTCCC TGAATTCTAT CCACAGCTCC CCGGGCCCCA AACGCTCCAC CAACACCCTA
    AAAAAGTGGC TGACGAGCCC TGTGAGGCGA CTGAACAGCG GGAAGGGCGA GAGCAACATC
    AAGAAGCAAA AGAAAAGAGA CGGGCGCAAC CTCGGCTCCC CCAGAGCTGG GGACGAGACC
    ACCCCACAGG GAGACAGCGC CGATGAGAAG GGCAAGAAGG GGTGGGACGA AGAGGCTGAC
    GACGAATCCC ACACTCCGCT TCCTCCGCCA ATGAAGATCT TTGATAATGA CCCCACTCCG
    GACGACAAGT CCTCCCTGCT TGTGGTCCGG CAGAGCTCAG CGGAGGTGCC TAGTGCGGCT
    GAACTGGTCA CTGCCATAGA GAATCTGGTC AGGAACAAGA TGACCATTGA CGGAGGTTCT
    TATCGGGGCG CACAGAACAT AAATGAAGGA TTTGTGAATG AGGGTTTGGC CCCCTCGCCA
    GCTCCCAGGA ACCACGAAGA GGAGCAAAAA GCCAAAGCAC TCAGGGGCAG GATGTTTGTA
    CTGAATGAGC TGGTACAGAC GGAGAAGGAC TATGTCCGGG ATCTTGGGTT TATGGTAGAG
    ACGTACATCC CCAAGATGGA GGAGAGGGGG ACCCCGGAAG ATATGAACGG AAAAGACAAG
    ATCGTATTCG GCAACATCCA CCAGATCTAC GATTGGCACA AAGACTTTTA CTTGGCAGAA
    CTAGAGAAAT GCCTATTGGA GCCGGAGAGG TTGGCGCAGT TATTTATAAA ACATGAGAGG
    CGGCTGCACA TGTATGTGGT TTATTGCCAA AACAAACCGC GCTCGGAGTT TGTCGTTGCC
    GAATATGACG CCTACTTTGA GGATCTCATG CAGGAGGTGA ATCCCAGATT TACTGTCAGT
    GATTTTCTCA TCAAACCGAT CCAAAGGATA ACTAAGTACC AACTGCTGCT AAAGGACTTC
    CTTAAGTACA GTCAGAAGGC GGGTTTGGAG TGTTCAGAAA TCGAGAAAGC CGTGGAACTA
    ATGTGCCTTG TCCCGAAACG CTGCAACGAC ATGATGAACC TGGGGCGCCT GCAGGGATTC
    GAGGGGAAGC TGACAGCCCA GGGGAAGCTG TTGCAGCAGG ACACCTTCTA TGTGATCGAG
    CAGGAATCGG GGGTCCAGTC TCGGACGAAG GAAAGGCGGG TTTTCCTGTT CGAGCAGATT
    GTTATTTTCA GCGAGCTCCT GCGGAAAGGT TCCTCCACGC CGGGGTACAT GTTCAAAAGG
    GGCATAAAGA TGAATTATCT CATCTTAGAA GAGAACATTG ACAACGACCC GTGCAAATTC
    GCCATTCGGA GCCGCGAGAC GTCGGAGCGG GTGATCCTGC AAGCGGCCAA CGCCGACATT
    AAACAGGCCT GGGTGCAGGA CATCGGGCAA GTGCTGGAGA CCCAGAGGGA CTTCCTAAAC
    GCCCTGCAAT CGCCAATAGA ATACCAGCGG AAGGAGAGCA ACGCAGCCAT GAACAAGCCT
    CAGATTGGCC GCGGGCCGTC CCCCACCGTT AGGCCTCTCT CCTCTACGCC CGTAGGGTGT
    GAAAAAGATG TCACCATCCC AGTGCGGAAC CAGTCGCTAC CAGCGCGGAA AATACCTACC
    TCTAACGGCA GCGCCGGGTA TGAAATGTAC CCACGGGCGG AGAAATGTGA CCCAAACCAG
    AGCGAGCTGA GCGGCTGCAA TGGGACCAAC TCCATGGTGG TTGTACAGGA TTACTATGCA
    CTAAAGGAGA ATGAGATCTG CGTGTACCAC GGGGAGTTGG TACAGATTCT GGCCATCAAT
    CAGCAGAACA TGTTCCTGGT ATTCCGGCCG TCCAATGACC ATTCCCCGGC AGCGGAAGGC
    TGGATCCCGG GATCAGTTTT GGGCCACATT TCTAAACCTT CCGCAGAAAA TAGCGACAGA
    TCCATCAAGA CGTCATGCTC TTGGCACACG CTCCGCATGA GAAAACGGGA CGGGACGGAG
    TCCAACGGCC CATGTAAGAG GCAAGAGGGC CTGGGCAGCA TGGCGAAAGA CCCCAATAAC
    AGTTCAGAGG AGTCTGAATG CGATGACCTT GACCCCAGTG CTAGTATGGA GATAATGAAC
    CCTAATTTCA TCCAGGAAGT GGCCCCTGAG TTCCTGGTGT CCCTGGTGGA TGTCATGTGT
    GTTCTTGGGG ACACAGTCAC CCTACAGTGC AAAGTGTGCG GCCGGCCGAA GCCCAGTGTC
    ACCTGGAAGG GACCCGACCA GAGCATCTTG GATGGGAACA GCAACACCTA CTCCATGTCA
    ACCAGTGAGT CTGGAGACAT CAAGCTGAAG ATCTGCCACG TGATGCCCCA GGATAGTGGC
    ATCTACACGT GTGTGGCCAG TAATGAGCTG GGGGCAACCT CGACATCTGC CACCATCAAA
    GTGCAAGGGG TCCCTGGGGC CCCCAGCCAG CCGCTGGCTC AGGAGAGAAG CAGCACCTCC
    GTCATCCTCC GCTGGCTGCC ACCTACCAGC ACCGGCAATT GCACTATATC CGGCTACACT
    GTGGAATACA AGGAGGAGGG TGCCCAACTA TGGCAGCAGT CAGTCGCCTC TACCCTGGAT
    ACTTACCTCG TCCTAGAAGA CCTGTGCCCT GGGGCCCGGT ACCAGTTCCG CGTGAGCGCC
    AGTAACCCCT GGGGTATAAG TCTGCCGAGT GAGCCTTCCG AGCCAGTCAG GCTTCCAGAA
    ACTGATACCA ATTCCAACGG ATCAACAATT TCATGGAAAG GAAACTTTGA GCAAGTCTAC
    ACAGAGCTGC ATGAGATTGG AAGGGGCAGG TTCTCCATAG TGAAGAAATG TGCCAAGAAA
    CAGAGCAGGA AGGACGTGGC GGCCAAATTC ATCAGCAAGA AGCTAAAGAA GCGAGAGCAG
    GCGGCGCACG AGGCGACACT TCTCCAGCAC CTACAGCACC CACAGTACAT TACCATCCAT
    GACACCTTTG AGTCGGACGG TGCCTACATC CTCATCCTGG ACCTCATGGA AGATGGCCGC
    TTGCTGGATT ACCTCATGAA ACACGACGAG CTGATGGAAG AGAAAGTGGC GTTCTACATG
    CGAGACATCA TGGAGGCGCT GCAGTACCTG CACAGCTGCC GCGTGGCGCA TTTAGACATC
    AAGCCGGAGA ATCTCCTCAT AGACCTGCGC ATCCCGGTGC CCAGAGTGAA GATCATCGAC
    CTGGAGGACG CCGTGCAGAT AACGGGGCAC CATTATATCC ATCAGCTGCT GGGGAACCCC
    GAGTTTGCTG CCCCCGAGCT TATCCAAGGG AGCCCCGTCT CTCTGGGCAC AGACATCTGG
    AGCTTGGGGG TGCTGACCTA CGTCATGCTG AGCGGGGTCT CCCCGTTCCT GGACGAGAGC
    AGCGAGGAGA CCTGCGCCAA CATCTGCCGG GTGGACTTCA GTTTCCCCCA GGAGTATTTC
    TCGGGGGTCA GTCAGGCTGC CCAGGACTTC ATCAAGGCCA CGTTGCAAGG GGACCCTCGC
    TGGAGACCCA CCATCGCCAC CTGTCTGCAG CACCCATGGC TGCAGCCTCA TAACAACACT
    TACTCCAAAT TACCCCTGGA CACCTCCCGC CTGGCTGGCT TCATTGAGAG GCGGAGCCAT
    CAAAACGACG CCCAGCCATT GGTCAAAACC AAGAGTCTCA TCATTAGCAG GAACGTGGTG
    GGAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748495.1
    CDS18..6206
    Translation

    Target ORF information:

    RefSeq Version XM_031892635.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kalirin RhoGEF kinase (kalrn), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892635.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGGCGAGT CGATGCTGAA TGCCAGTCTG GTTAATGCCA GTTCACTGTC GGAAGCTGAG 
    CAGTTGCAGC GAGAACACGA GCAGTTTCAG ATGGCTATTG AGTCCCTTTT CCACGCCAGC
    TCTCTGCAGA AGACTCACCA AAGTGCCCTG CAGGTCCAGC AGAAAGCAGA GGCGCTTCTA
    CAGGCCGGGC ACTACGACGC CGACGCCATC CGGGAATGCG CCGAGAAGGT GGCGATGCAC
    TGGCAGCAAC TCATGCTCAA GATGGAGGAC AGACTCAAGC TAGTGAATGC CTCTGTGGCC
    TTCTATAAAA CCTCCGAGCA GGTGTGCAGC GTTCTGGAGA GCCTGGAGCA GGAGTACCGC
    AGGGATGAGG ACTGGTGTCG GGGCCACGAT AAGCTGGGAC CCTCCACTGA GTCGGACCAC
    GTGATGCCGC TGATCAGTAA ACACCTGGAG CAGAAGGAGG CTTTCCTTAA GGCTTGTACG
    TTAGCGCGGA GGAACGCGGA AGTCTTTCTG AAGTACATTC ACCGTAACAA CGTGAGCATG
    CCCGGCGTGG CCCCCCACAC GCGCGTCCCC GAGCAGCAAG TTAAAGCCAT ACTGAGCGAG
    CTCCTGCAAA GGGAGAACCG GGTGCTGCAC TTTTGGACCC TGAAGAAGCG GCGGTTGGAC
    CAGTGCCAGC AGTACATTGT GTTTGAGAGG AGCGCCAAGC AGGCTCTGGA CTGGATTCAG
    GAAGCCGGGG AGCATTATCT CTCCACTCGC ATCTCCCCTG GGGAATGTAT TGAGGAGACT
    CAGGAGCTGC TGAAGGAATA CGAGGAATTC CGGATACCTG CCAAGCAAAC GAAGGAGAAG
    GTGCGGCTGC TGATCCAGCT GGCAGACAGC TTTGTGGAGA AGGGCCACGT TCACGCCACC
    GAGCTGAAGA AATGGGTCAC TACTGTCAAC AAACACTACC GGGAATTCGC TCTGCGTATG
    GGCAAATACC ATTGCGGCCT GCAGCGAGCC CTGGGCATCT GCACTGAGGA CACAAAGGAC
    CTGGAGTTGG ACATTGTCCC TGCGATTCTG ACTGACCCGG AGGTGAAGCT GCGCGACGCC
    GGCCATGAAC TCAACGAGGA GAAGAGAAAA TCCGCCAGGA AAAAAGAATT CATCATGGCG
    GAACTTCTAC AGACAGAGAA AGCGTATGTG CGGGACTTGC ACGAGTGTAT GGAGACCTAC
    CTGTGGGAAA TGACCAGCGG AGTGGAGGAA ATTCCGCCCG GAATAGTGAA TAAGGAGCAC
    ATCATCTTTG GGAACATTCA GGAAATATAC GACTTTCACA ATAACATCTT CCTGAAAGAG
    CTGGAGAAGT ACGAGCAGCT GCCGGAGGAC GTGGGACATT GTTTTGTCAC TTGGGCCGAC
    AAGTTCCACA TGTACGTGAC CTACTGTAAG AACAAACCCG ACTCCAGTCA GCTGATCCTG
    GAGCACGCCG GCTCCTTCTT TGACGAGATC CAGCAGCGAC ACGGCCTGGC CAACTCCATC
    TCCTCCTACC TCATCAAACC CGTCCAGCGG ATCACTAAGT ACCAGCTGCT TCTCAAGGAG
    CTTCTGACCT GCTGTGAGGA AGGGAAAGGG GAGATAAAGG ACGGGCTGGA GGTGATGCTG
    AGTGTGCCCA AGCGGGCCAA TGATGCCATG CACGTCAGCA TGCTGGAAGG ATTTGACGAA
    AACCTGGACG TTCAAGGGGA ACTTATCCTG CAAGATTCCT TCCAAGTCTG GGACCCCAAA
    TCCCTCATCC GCAAGGGGCG CGACCGGCAC CTCTTCCTCT TCGAAATCTC CCTCGTCTTC
    AGCAAAGAGA TCAAAGACTC CACCGGCCAC ACCAAATACG TCTTTAAGAA CAAACTGCTG
    ACCTCGGAAC TGGGGGTGAC GGAGCACGTA GAGGGGGACC CCTGTAAGTT TGCCCTATGG
    TCGGGGCGCA CCCCATCATC CGATAACAAG ACAGTGCTAA AGGCATCTAG TATCGAGGTG
    AAGCAGGAAT GGATCAAGAA CATCCGGGAA GTCATTCAGG AAAGGATGAT CCATTTGAAA
    GGAGCCTTGA AAGAACCCAT CCAGCCCCCC AAAACTCCAG CAAAGCAGCG CAACAACAGC
    AAAAGGGACG TACTGGAGGA CGCCGATAGC CAGGGGGACG GCAGCAGCCA ACCGGACACC
    ATCTCCATCG CCTCCAGAAC CTCCCAGAAC ACCATGGACA GCGACAAGTT ATCTGGGGGC
    TGTGAACTGA CGACGGTTCT GCAGGATTTC ATCGCTCTCC ACAACAATGA GCTGAGCATT
    CAGGTGGGGC AAACCGTGGA GCTGCTGGAG AGGCCGAGCG ACCGGCCTGG ATGGTGCTTA
    GTCCGGACCA CCGACCGGAG CCCCCCGCAG GAAGGGCTGG TACCCAGCAG CGCCCTGTGC
    ATCTCCCACT CGCGGAGCAG CGTCGAAATG GAGTGTTTCT TCCCTTCAGG CAAAGATTCA
    TTTTCTGTCT CCGCGGCGGA AAATGGGGGG AAGTCCGAGT CGGTGGCCAA TCTGCAGCCC
    CAGGCGTCCC TGAATTCTAT CCACAGCTCC CCGGGCCCCA AACGCTCCAC CAACACCCTA
    AAAAAGTGGC TGACGAGCCC TGTGAGGCGA CTGAACAGCG GGAAGGGCGA GAGCAACATC
    AAGAAGCAAA AGAAAAGAGA CGGGCGCAAC CTCGGCTCCC CCAGAGCTGG GGACGAGACC
    ACCCCACAGG GAGACAGCGC CGATGAGAAG GGCAAGAAGG GGTGGGACGA AGAGGCTGAC
    GACGAATCCC ACACTCCGCT TCCTCCGCCA ATGAAGATCT TTGATAATGA CCCCACTCCG
    GACGACAAGT CCTCCCTGCT TGTGGTCCGG CAGAGCTCAG CGGAGGTGCC TAGTGCGGCT
    GAACTGGTCA CTGCCATAGA GAATCTGGTC AGGAACAAGA TGACCATTGA CGGAGGTTCT
    TATCGGGGCG CACAGAACAT AAATGAAGGA TTTGTGAATG AGGGTTTGGC CCCCTCGCCA
    GCTCCCAGGA ACCACGAAGA GGAGCAAAAA GCCAAAGCAC TCAGGGGCAG GATGTTTGTA
    CTGAATGAGC TGGTACAGAC GGAGAAGGAC TATGTCCGGG ATCTTGGGTT TATGGTAGAG
    ACGTACATCC CCAAGATGGA GGAGAGGGGG ACCCCGGAAG ATATGAACGG AAAAGACAAG
    ATCGTATTCG GCAACATCCA CCAGATCTAC GATTGGCACA AAGACTTTTA CTTGGCAGAA
    CTAGAGAAAT GCCTATTGGA GCCGGAGAGG TTGGCGCAGT TATTTATAAA ACATGAGAGG
    CGGCTGCACA TGTATGTGGT TTATTGCCAA AACAAACCGC GCTCGGAGTT TGTCGTTGCC
    GAATATGACG CCTACTTTGA GGATCTCATG CAGGAGGTGA ATCCCAGATT TACTGTCAGT
    GATTTTCTCA TCAAACCGAT CCAAAGGATA ACTAAGTACC AACTGCTGCT AAAGGACTTC
    CTTAAGTACA GTCAGAAGGC GGGTTTGGAG TGTTCAGAAA TCGAGAAAGC CGTGGAACTA
    ATGTGCCTTG TCCCGAAACG CTGCAACGAC ATGATGAACC TGGGGCGCCT GCAGGGATTC
    GAGGGGAAGC TGACAGCCCA GGGGAAGCTG TTGCAGCAGG ACACCTTCTA TGTGATCGAG
    CAGGAATCGG GGGTCCAGTC TCGGACGAAG GAAAGGCGGG TTTTCCTGTT CGAGCAGATT
    GTTATTTTCA GCGAGCTCCT GCGGAAAGGT TCCTCCACGC CGGGGTACAT GTTCAAAAGG
    GGCATAAAGA TGAATTATCT CATCTTAGAA GAGAACATTG ACAACGACCC GTGCAAATTC
    GCCATTCGGA GCCGCGAGAC GTCGGAGCGG GTGATCCTGC AAGCGGCCAA CGCCGACATT
    AAACAGGCCT GGGTGCAGGA CATCGGGCAA GTGCTGGAGA CCCAGAGGGA CTTCCTAAAC
    GCCCTGCAAT CGCCAATAGA ATACCAGCGG AAGGAGAGCA ACGCAGCCAT GAACAAGCCT
    CAGATTGGCC GCGGGCCGTC CCCCACCGTT AGGCCTCTCT CCTCTACGCC CGTAGGGTGT
    GAAAAAGATG TCACCATCCC AGTGCGGAAC CAGTCGCTAC CAGCGCGGAA AATACCTACC
    TCTAACGGCA GCGCCGGGTA TGAAATGTAC CCACGGGCGG AGAAATGTGA CCCAAACCAG
    AGCGAGCTGA GCGGCTGCAA TGGGACCAAC TCCATGGTGG TTGTACAGGA TTACTATGCA
    CTAAAGGAGA ATGAGATCTG CGTGTACCAC GGGGAGTTGG TACAGATTCT GGCCATCAAT
    CAGCAGAACA TGTTCCTGGT ATTCCGGCCG TCCAATGACC ATTCCCCGGC AGCGGAAGGC
    TGGATCCCGG GATCAGTTTT GGGCCACATT TCTAAACCTT CCGCAGAAAA TAGCGACAGA
    TCCATCAAGA CGTCATGCTC TTGGCACACG CTCCGCATGA GAAAACGGGA CGGGACGGAG
    TCCAACGGCC CATGTAAGAG GCAAGAGGGC CTGGGCAGCA TGGCGAAAGA CCCCAATAAC
    AGTTCAGAGG AGTCTGAATG CGATGACCTT GACCCCAGTG CTAGTATGGA GATAATGAAC
    CCTAATTTCA TCCAGGAAGT GGCCCCTGAG TTCCTGGTGT CCCTGGTGGA TGTCATGTGT
    GTTCTTGGGG ACACAGTCAC CCTACAGTGC AAAGTGTGCG GCCGGCCGAA GCCCAGTGTC
    ACCTGGAAGG GACCCGACCA GAGCATCTTG GATGGGAACA GCAACACCTA CTCCATGTCA
    ACCAGTGAGT CTGGAGACAT CAAGCTGAAG ATCTGCCACG TGATGCCCCA GGATAGTGGC
    ATCTACACGT GTGTGGCCAG TAATGAGCTG GGGGCAACCT CGACATCTGC CACCATCAAA
    GTGCAAGGGG TCCCTGGGGC CCCCAGCCAG CCGCTGGCTC AGGAGAGAAG CAGCACCTCC
    GTCATCCTCC GCTGGCTGCC ACCTACCAGC ACCGGCAATT GCACTATATC CGGCTACACT
    GTGGAATACA AGGAGGAGGG TGCCCAACTA TGGCAGCAGT CAGTCGCCTC TACCCTGGAT
    ACTTACCTCG TCCTAGAAGA CCTGTGCCCT GGGGCCCGGT ACCAGTTCCG CGTGAGCGCC
    AGTAACCCCT GGGGTATAAG TCTGCCGAGT GAGCCTTCCG AGCCAGTCAG GCTTCCAGAA
    ACTGATACCA ATTCCAACGG ATCAACAATT TCATGGAAAG GAAACTTTGA GCAAGTCTAC
    ACAGAGCTGC ATGAGATTGG AAGGGGCAGG TTCTCCATAG TGAAGAAATG TGCCAAGAAA
    CAGAGCAGGA AGGACGTGGC GGCCAAATTC ATCAGCAAGA AGCTAAAGAA GCGAGAGCAG
    GCGGCGCACG AGGCGACACT TCTCCAGCAC CTACAGCACC CACAGTACAT TACCATCCAT
    GACACCTTTG AGTCGGACGG TGCCTACATC CTCATCCTGG ACCTCATGGA AGATGGCCGC
    TTGCTGGATT ACCTCATGAA ACACGACGAG CTGATGGAAG AGAAAGTGGC GTTCTACATG
    CGAGACATCA TGGAGGCGCT GCAGTACCTG CACAGCTGCC GCGTGGCGCA TTTAGACATC
    AAGCCGGAGA ATCTCCTCAT AGACCTGCGC ATCCCGGTGC CCAGAGTGAA GATCATCGAC
    CTGGAGGACG CCGTGCAGAT AACGGGGCAC CATTATATCC ATCAGCTGCT GGGGAACCCC
    GAGTTTGCTG CCCCCGAGCT TATCCAAGGG AGCCCCGTCT CTCTGGGCAC AGACATCTGG
    AGCTTGGGGG TGCTGACCTA CGTCATGCTG AGCGGGGTCT CCCCGTTCCT GGACGAGAGC
    AGCGAGGAGA CCTGCGCCAA CATCTGCCGG GTGGACTTCA GTTTCCCCCA GGAGTATTTC
    TCGGGGGTCA GTCAGGCTGC CCAGGACTTC ATCAAGGCCA CGTTGCAAGG GGACCCTCGC
    TGGAGACCCA CCATCGCCAC CTGTCTGCAG CACCCATGGC TGCAGCCTCA TAACAACACT
    TACTCCAAAT TACCCCTGGA CACCTCCCGC CTGGCTGGCT TCATTGAGAG GCGGAGCCAT
    CAAAACGACG CCCAGCCATT GGTCAAAACC AAGAGTCTCA TCATTAGCAG GAACGTGGTG
    GGAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa66018
    Clone ID Related Accession (Same CDS sequence) XM_031892636.1
    Accession Version XM_031892636.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6186bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kalirin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGGCGAGT CGATGCTGAA TGCCAGTCTG GTTAATGCCA GTTCACTGTC GGAAGCTGAG 
    CAGTTGCAGC GAGAACACGA GCAGTTTCAG ATGGCTATTG AGTCCCTTTT CCACGCCAGC
    TCTCTGCAGA AGACTCACCA AAGTGCCCTG CAGGTCCAGC AGAAAGCAGA GGCGCTTCTA
    CAGGCCGGGC ACTACGACGC CGACGCCATC CGGGAATGCG CCGAGAAGGT GGCGATGCAC
    TGGCAGCAAC TCATGCTCAA GATGGAGGAC AGACTCAAGC TAGTGAATGC CTCTGTGGCC
    TTCTATAAAA CCTCCGAGCA GGTGTGCAGC GTTCTGGAGA GCCTGGAGCA GGAGTACCGC
    AGGGATGAGG ACTGGTGTCG GGGCCACGAT AAGCTGGGAC CCTCCACTGA GTCGGACCAC
    GTGATGCCGC TGATCAGTAA ACACCTGGAG CAGAAGGAGG CTTTCCTTAA GGCTTGTACG
    TTAGCGCGGA GGAACGCGGA AGTCTTTCTG AAGTACATTC ACCGTAACAA CGTGAGCATG
    CCCGGCGTGG CCCCCCACAC GCGCGTCCCC GAGCAGCAAG TTAAAGCCAT ACTGAGCGAG
    CTCCTGCAAA GGGAGAACCG GGTGCTGCAC TTTTGGACCC TGAAGAAGCG GCGGTTGGAC
    CAGTGCCAGC AGTACATTGT GTTTGAGAGG AGCGCCAAGC AGGCTCTGGA CTGGATTCAG
    GAAGCCGGGG AGCATTATCT CTCCACTCGC ATCTCCCCTG GGGAATGTAT TGAGGAGACT
    CAGGAGCTGC TGAAGGAATA CGAGGAATTC CGGATACCTG CCAAGCAAAC GAAGGAGAAG
    GTGCGGCTGC TGATCCAGCT GGCAGACAGC TTTGTGGAGA AGGGCCACGT TCACGCCACC
    GAGCTGAAGA AATGGGTCAC TACTGTCAAC AAACACTACC GGGAATTCGC TCTGCGTATG
    GGCAAATACC ATTGCGGCCT GCAGCGAGCC CTGGGCATCT GCACTGAGGA CACAAAGGAC
    CTGGAGTTGG ACATTGTCCC TGCGATTCTG ACTGACCCGG AGGTGAAGCT GCGCGACGCC
    GGCCATGAAC TCAACGAGGA GAAGAGAAAA TCCGCCAGGA AAAAAGAATT CATCATGGCG
    GAACTTCTAC AGACAGAGAA AGCGTATGTG CGGGACTTGC ACGAGTGTAT GGAGACCTAC
    CTGTGGGAAA TGACCAGCGG AGTGGAGGAA ATTCCGCCCG GAATAGTGAA TAAGGAGCAC
    ATCATCTTTG GGAACATTCA GGAAATATAC GACTTTCACA ATAACATCTT CCTGAAAGAG
    CTGGAGAAGT ACGAGCAGCT GCCGGAGGAC GTGGGACATT GTTTTGTCAC TTGGGCCGAC
    AAGTTCCACA TGTACGTGAC CTACTGTAAG AACAAACCCG ACTCCAGTCA GCTGATCCTG
    GAGCACGCCG GCTCCTTCTT TGACGAGATC CAGCAGCGAC ACGGCCTGGC CAACTCCATC
    TCCTCCTACC TCATCAAACC CGTCCAGCGG ATCACTAAGT ACCAGCTGCT TCTCAAGGAG
    CTTCTGACCT GCTGTGAGGA AGGGAAAGGG GAGATAAAGG ACGGGCTGGA GGTGATGCTG
    AGTGTGCCCA AGCGGGCCAA TGATGCCATG CACGTCAGCA TGCTGGAAGG ATTTGACGAA
    AACCTGGACG TTCAAGGGGA ACTTATCCTG CAAGATTCCT TCCAAGTCTG GGACCCCAAA
    TCCCTCATCC GCAAGGGGCG CGACCGGCAC CTCTTCCTCT TCGAAATCTC CCTCGTCTTC
    AGCAAAGAGA TCAAAGACTC CACCGGCCAC ACCAAATACG TCTTTAAGAA CAAACTGCTG
    ACCTCGGAAC TGGGGGTGAC GGAGCACGTA GAGGGGGACC CCTGTAAGTT TGCCCTATGG
    TCGGGGCGCA CCCCATCATC CGATAACAAG ACAGTGCTAA AGGCATCTAG TATCGAGGTG
    AAGCAGGAAT GGATCAAGAA CATCCGGGAA GTCATTCAGG AAAGGATGAT CCATTTGAAA
    GGAGCCTTGA AAGAACCCAT CCAGCCCCCC AAAACTCCAG CAAAGCAGCG CAACAACAGC
    AAAAGGGACG TACTGGAGGA CGCCGATAGC CAGGGGGACG GCAGCAGCCA ACCGGACACC
    ATCTCCATCG CCTCCAGAAC CTCCCAGAAC ACCATGGACA GCGACAAGTT ATCTGGGGGC
    TGTGAACTGA CGACGGTTCT GCAGGATTTC ATCGCTCTCC ACAACAATGA GCTGAGCATT
    CAGGTGGGGC AAACCGTGGA GCTGCTGGAG AGGCCGAGCG ACCGGCCTGG ATGGTGCTTA
    GTCCGGACCA CCGACCGGAG CCCCCCGCAG GAAGGGCTGG TACCCAGCAG CGCCCTGTGC
    ATCTCCCACT CGCGGAGCAG CGTCGAAATG GAGTGTTTCT TCCCTTCAGG CAAAGATTCA
    TTTTCTGTCT CCGCGGCGGA AAATGGGGGG AAGTCCGAGT CGGTGGCCAA TCTGCAGCCC
    CAGGCGTCCC TGAATTCTAT CCACAGCTCC CCGGGCCCCA AACGCTCCAC CAACACCCTA
    AAAAAGTGGC TGACGAGCCC TGTGAGGCGA CTGAACAGCG GGAAGGGCGA GAGCAACATC
    AAGAAGCAAA AGAAAAGAGA CGGGCGCAAC CTCGGCTCCC CCAGAGCTGG GGACGAGACC
    ACCCCACAGG GAGACAGCGC CGATGAGGGC AAGAAGGGGT GGGACGAAGA GGCTGACGAC
    GAATCCCACA CTCCGCTTCC TCCGCCAATG AAGATCTTTG ATAATGACCC CACTCCGGAC
    GACAAGTCCT CCCTGCTTGT GGTCCGGCAG AGCTCAGCGG AGGTGCCTAG TGCGGCTGAA
    CTGGTCACTG CCATAGAGAA TCTGGTCAGG AACAAGATGA CCATTGACGG AGGTTCTTAT
    CGGGGCGCAC AGAACATAAA TGAAGGATTT GTGAATGAGG GTTTGGCCCC CTCGCCAGCT
    CCCAGGAACC ACGAAGAGGA GCAAAAAGCC AAAGCACTCA GGGGCAGGAT GTTTGTACTG
    AATGAGCTGG TACAGACGGA GAAGGACTAT GTCCGGGATC TTGGGTTTAT GGTAGAGACG
    TACATCCCCA AGATGGAGGA GAGGGGGACC CCGGAAGATA TGAACGGAAA AGACAAGATC
    GTATTCGGCA ACATCCACCA GATCTACGAT TGGCACAAAG ACTTTTACTT GGCAGAACTA
    GAGAAATGCC TATTGGAGCC GGAGAGGTTG GCGCAGTTAT TTATAAAACA TGAGAGGCGG
    CTGCACATGT ATGTGGTTTA TTGCCAAAAC AAACCGCGCT CGGAGTTTGT CGTTGCCGAA
    TATGACGCCT ACTTTGAGGA TCTCATGCAG GAGGTGAATC CCAGATTTAC TGTCAGTGAT
    TTTCTCATCA AACCGATCCA AAGGATAACT AAGTACCAAC TGCTGCTAAA GGACTTCCTT
    AAGTACAGTC AGAAGGCGGG TTTGGAGTGT TCAGAAATCG AGAAAGCCGT GGAACTAATG
    TGCCTTGTCC CGAAACGCTG CAACGACATG ATGAACCTGG GGCGCCTGCA GGGATTCGAG
    GGGAAGCTGA CAGCCCAGGG GAAGCTGTTG CAGCAGGACA CCTTCTATGT GATCGAGCAG
    GAATCGGGGG TCCAGTCTCG GACGAAGGAA AGGCGGGTTT TCCTGTTCGA GCAGATTGTT
    ATTTTCAGCG AGCTCCTGCG GAAAGGTTCC TCCACGCCGG GGTACATGTT CAAAAGGGGC
    ATAAAGATGA ATTATCTCAT CTTAGAAGAG AACATTGACA ACGACCCGTG CAAATTCGCC
    ATTCGGAGCC GCGAGACGTC GGAGCGGGTG ATCCTGCAAG CGGCCAACGC CGACATTAAA
    CAGGCCTGGG TGCAGGACAT CGGGCAAGTG CTGGAGACCC AGAGGGACTT CCTAAACGCC
    CTGCAATCGC CAATAGAATA CCAGCGGAAG GAGAGCAACG CAGCCATGAA CAAGCCTCAG
    ATTGGCCGCG GGCCGTCCCC CACCGTTAGG CCTCTCTCCT CTACGCCCGT AGGGTGTGAA
    AAAGATGTCA CCATCCCAGT GCGGAACCAG TCGCTACCAG CGCGGAAAAT ACCTACCTCT
    AACGGCAGCG CCGGGTATGA AATGTACCCA CGGGCGGAGA AATGTGACCC AAACCAGAGC
    GAGCTGAGCG GCTGCAATGG GACCAACTCC ATGGTGGTTG TACAGGATTA CTATGCACTA
    AAGGAGAATG AGATCTGCGT GTACCACGGG GAGTTGGTAC AGATTCTGGC CATCAATCAG
    CAGAACATGT TCCTGGTATT CCGGCCGTCC AATGACCATT CCCCGGCAGC GGAAGGCTGG
    ATCCCGGGAT CAGTTTTGGG CCACATTTCT AAACCTTCCG CAGAAAATAG CGACAGATCC
    ATCAAGACGT CATGCTCTTG GCACACGCTC CGCATGAGAA AACGGGACGG GACGGAGTCC
    AACGGCCCAT GTAAGAGGCA AGAGGGCCTG GGCAGCATGG CGAAAGACCC CAATAACAGT
    TCAGAGGAGT CTGAATGCGA TGACCTTGAC CCCAGTGCTA GTATGGAGAT AATGAACCCT
    AATTTCATCC AGGAAGTGGC CCCTGAGTTC CTGGTGTCCC TGGTGGATGT CATGTGTGTT
    CTTGGGGACA CAGTCACCCT ACAGTGCAAA GTGTGCGGCC GGCCGAAGCC CAGTGTCACC
    TGGAAGGGAC CCGACCAGAG CATCTTGGAT GGGAACAGCA ACACCTACTC CATGTCAACC
    AGTGAGTCTG GAGACATCAA GCTGAAGATC TGCCACGTGA TGCCCCAGGA TAGTGGCATC
    TACACGTGTG TGGCCAGTAA TGAGCTGGGG GCAACCTCGA CATCTGCCAC CATCAAAGTG
    CAAGGGGTCC CTGGGGCCCC CAGCCAGCCG CTGGCTCAGG AGAGAAGCAG CACCTCCGTC
    ATCCTCCGCT GGCTGCCACC TACCAGCACC GGCAATTGCA CTATATCCGG CTACACTGTG
    GAATACAAGG AGGAGGGTGC CCAACTATGG CAGCAGTCAG TCGCCTCTAC CCTGGATACT
    TACCTCGTCC TAGAAGACCT GTGCCCTGGG GCCCGGTACC AGTTCCGCGT GAGCGCCAGT
    AACCCCTGGG GTATAAGTCT GCCGAGTGAG CCTTCCGAGC CAGTCAGGCT TCCAGAAACT
    GATACCAATT CCAACGGATC AACAATTTCA TGGAAAGGAA ACTTTGAGCA AGTCTACACA
    GAGCTGCATG AGATTGGAAG GGGCAGGTTC TCCATAGTGA AGAAATGTGC CAAGAAACAG
    AGCAGGAAGG ACGTGGCGGC CAAATTCATC AGCAAGAAGC TAAAGAAGCG AGAGCAGGCG
    GCGCACGAGG CGACACTTCT CCAGCACCTA CAGCACCCAC AGTACATTAC CATCCATGAC
    ACCTTTGAGT CGGACGGTGC CTACATCCTC ATCCTGGACC TCATGGAAGA TGGCCGCTTG
    CTGGATTACC TCATGAAACA CGACGAGCTG ATGGAAGAGA AAGTGGCGTT CTACATGCGA
    GACATCATGG AGGCGCTGCA GTACCTGCAC AGCTGCCGCG TGGCGCATTT AGACATCAAG
    CCGGAGAATC TCCTCATAGA CCTGCGCATC CCGGTGCCCA GAGTGAAGAT CATCGACCTG
    GAGGACGCCG TGCAGATAAC GGGGCACCAT TATATCCATC AGCTGCTGGG GAACCCCGAG
    TTTGCTGCCC CCGAGCTTAT CCAAGGGAGC CCCGTCTCTC TGGGCACAGA CATCTGGAGC
    TTGGGGGTGC TGACCTACGT CATGCTGAGC GGGGTCTCCC CGTTCCTGGA CGAGAGCAGC
    GAGGAGACCT GCGCCAACAT CTGCCGGGTG GACTTCAGTT TCCCCCAGGA GTATTTCTCG
    GGGGTCAGTC AGGCTGCCCA GGACTTCATC AAGGCCACGT TGCAAGGGGA CCCTCGCTGG
    AGACCCACCA TCGCCACCTG TCTGCAGCAC CCATGGCTGC AGCCTCATAA CAACACTTAC
    TCCAAATTAC CCCTGGACAC CTCCCGCCTG GCTGGCTTCA TTGAGAGGCG GAGCCATCAA
    AACGACGCCC AGCCATTGGT CAAAACCAAG AGTCTCATCA TTAGCAGGAA CGTGGTGGGA
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748496.1
    CDS19..6204
    Translation

    Target ORF information:

    RefSeq Version XM_031892636.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kalirin RhoGEF kinase (kalrn), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892636.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGGCGAGT CGATGCTGAA TGCCAGTCTG GTTAATGCCA GTTCACTGTC GGAAGCTGAG 
    CAGTTGCAGC GAGAACACGA GCAGTTTCAG ATGGCTATTG AGTCCCTTTT CCACGCCAGC
    TCTCTGCAGA AGACTCACCA AAGTGCCCTG CAGGTCCAGC AGAAAGCAGA GGCGCTTCTA
    CAGGCCGGGC ACTACGACGC CGACGCCATC CGGGAATGCG CCGAGAAGGT GGCGATGCAC
    TGGCAGCAAC TCATGCTCAA GATGGAGGAC AGACTCAAGC TAGTGAATGC CTCTGTGGCC
    TTCTATAAAA CCTCCGAGCA GGTGTGCAGC GTTCTGGAGA GCCTGGAGCA GGAGTACCGC
    AGGGATGAGG ACTGGTGTCG GGGCCACGAT AAGCTGGGAC CCTCCACTGA GTCGGACCAC
    GTGATGCCGC TGATCAGTAA ACACCTGGAG CAGAAGGAGG CTTTCCTTAA GGCTTGTACG
    TTAGCGCGGA GGAACGCGGA AGTCTTTCTG AAGTACATTC ACCGTAACAA CGTGAGCATG
    CCCGGCGTGG CCCCCCACAC GCGCGTCCCC GAGCAGCAAG TTAAAGCCAT ACTGAGCGAG
    CTCCTGCAAA GGGAGAACCG GGTGCTGCAC TTTTGGACCC TGAAGAAGCG GCGGTTGGAC
    CAGTGCCAGC AGTACATTGT GTTTGAGAGG AGCGCCAAGC AGGCTCTGGA CTGGATTCAG
    GAAGCCGGGG AGCATTATCT CTCCACTCGC ATCTCCCCTG GGGAATGTAT TGAGGAGACT
    CAGGAGCTGC TGAAGGAATA CGAGGAATTC CGGATACCTG CCAAGCAAAC GAAGGAGAAG
    GTGCGGCTGC TGATCCAGCT GGCAGACAGC TTTGTGGAGA AGGGCCACGT TCACGCCACC
    GAGCTGAAGA AATGGGTCAC TACTGTCAAC AAACACTACC GGGAATTCGC TCTGCGTATG
    GGCAAATACC ATTGCGGCCT GCAGCGAGCC CTGGGCATCT GCACTGAGGA CACAAAGGAC
    CTGGAGTTGG ACATTGTCCC TGCGATTCTG ACTGACCCGG AGGTGAAGCT GCGCGACGCC
    GGCCATGAAC TCAACGAGGA GAAGAGAAAA TCCGCCAGGA AAAAAGAATT CATCATGGCG
    GAACTTCTAC AGACAGAGAA AGCGTATGTG CGGGACTTGC ACGAGTGTAT GGAGACCTAC
    CTGTGGGAAA TGACCAGCGG AGTGGAGGAA ATTCCGCCCG GAATAGTGAA TAAGGAGCAC
    ATCATCTTTG GGAACATTCA GGAAATATAC GACTTTCACA ATAACATCTT CCTGAAAGAG
    CTGGAGAAGT ACGAGCAGCT GCCGGAGGAC GTGGGACATT GTTTTGTCAC TTGGGCCGAC
    AAGTTCCACA TGTACGTGAC CTACTGTAAG AACAAACCCG ACTCCAGTCA GCTGATCCTG
    GAGCACGCCG GCTCCTTCTT TGACGAGATC CAGCAGCGAC ACGGCCTGGC CAACTCCATC
    TCCTCCTACC TCATCAAACC CGTCCAGCGG ATCACTAAGT ACCAGCTGCT TCTCAAGGAG
    CTTCTGACCT GCTGTGAGGA AGGGAAAGGG GAGATAAAGG ACGGGCTGGA GGTGATGCTG
    AGTGTGCCCA AGCGGGCCAA TGATGCCATG CACGTCAGCA TGCTGGAAGG ATTTGACGAA
    AACCTGGACG TTCAAGGGGA ACTTATCCTG CAAGATTCCT TCCAAGTCTG GGACCCCAAA
    TCCCTCATCC GCAAGGGGCG CGACCGGCAC CTCTTCCTCT TCGAAATCTC CCTCGTCTTC
    AGCAAAGAGA TCAAAGACTC CACCGGCCAC ACCAAATACG TCTTTAAGAA CAAACTGCTG
    ACCTCGGAAC TGGGGGTGAC GGAGCACGTA GAGGGGGACC CCTGTAAGTT TGCCCTATGG
    TCGGGGCGCA CCCCATCATC CGATAACAAG ACAGTGCTAA AGGCATCTAG TATCGAGGTG
    AAGCAGGAAT GGATCAAGAA CATCCGGGAA GTCATTCAGG AAAGGATGAT CCATTTGAAA
    GGAGCCTTGA AAGAACCCAT CCAGCCCCCC AAAACTCCAG CAAAGCAGCG CAACAACAGC
    AAAAGGGACG TACTGGAGGA CGCCGATAGC CAGGGGGACG GCAGCAGCCA ACCGGACACC
    ATCTCCATCG CCTCCAGAAC CTCCCAGAAC ACCATGGACA GCGACAAGTT ATCTGGGGGC
    TGTGAACTGA CGACGGTTCT GCAGGATTTC ATCGCTCTCC ACAACAATGA GCTGAGCATT
    CAGGTGGGGC AAACCGTGGA GCTGCTGGAG AGGCCGAGCG ACCGGCCTGG ATGGTGCTTA
    GTCCGGACCA CCGACCGGAG CCCCCCGCAG GAAGGGCTGG TACCCAGCAG CGCCCTGTGC
    ATCTCCCACT CGCGGAGCAG CGTCGAAATG GAGTGTTTCT TCCCTTCAGG CAAAGATTCA
    TTTTCTGTCT CCGCGGCGGA AAATGGGGGG AAGTCCGAGT CGGTGGCCAA TCTGCAGCCC
    CAGGCGTCCC TGAATTCTAT CCACAGCTCC CCGGGCCCCA AACGCTCCAC CAACACCCTA
    AAAAAGTGGC TGACGAGCCC TGTGAGGCGA CTGAACAGCG GGAAGGGCGA GAGCAACATC
    AAGAAGCAAA AGAAAAGAGA CGGGCGCAAC CTCGGCTCCC CCAGAGCTGG GGACGAGACC
    ACCCCACAGG GAGACAGCGC CGATGAGGGC AAGAAGGGGT GGGACGAAGA GGCTGACGAC
    GAATCCCACA CTCCGCTTCC TCCGCCAATG AAGATCTTTG ATAATGACCC CACTCCGGAC
    GACAAGTCCT CCCTGCTTGT GGTCCGGCAG AGCTCAGCGG AGGTGCCTAG TGCGGCTGAA
    CTGGTCACTG CCATAGAGAA TCTGGTCAGG AACAAGATGA CCATTGACGG AGGTTCTTAT
    CGGGGCGCAC AGAACATAAA TGAAGGATTT GTGAATGAGG GTTTGGCCCC CTCGCCAGCT
    CCCAGGAACC ACGAAGAGGA GCAAAAAGCC AAAGCACTCA GGGGCAGGAT GTTTGTACTG
    AATGAGCTGG TACAGACGGA GAAGGACTAT GTCCGGGATC TTGGGTTTAT GGTAGAGACG
    TACATCCCCA AGATGGAGGA GAGGGGGACC CCGGAAGATA TGAACGGAAA AGACAAGATC
    GTATTCGGCA ACATCCACCA GATCTACGAT TGGCACAAAG ACTTTTACTT GGCAGAACTA
    GAGAAATGCC TATTGGAGCC GGAGAGGTTG GCGCAGTTAT TTATAAAACA TGAGAGGCGG
    CTGCACATGT ATGTGGTTTA TTGCCAAAAC AAACCGCGCT CGGAGTTTGT CGTTGCCGAA
    TATGACGCCT ACTTTGAGGA TCTCATGCAG GAGGTGAATC CCAGATTTAC TGTCAGTGAT
    TTTCTCATCA AACCGATCCA AAGGATAACT AAGTACCAAC TGCTGCTAAA GGACTTCCTT
    AAGTACAGTC AGAAGGCGGG TTTGGAGTGT TCAGAAATCG AGAAAGCCGT GGAACTAATG
    TGCCTTGTCC CGAAACGCTG CAACGACATG ATGAACCTGG GGCGCCTGCA GGGATTCGAG
    GGGAAGCTGA CAGCCCAGGG GAAGCTGTTG CAGCAGGACA CCTTCTATGT GATCGAGCAG
    GAATCGGGGG TCCAGTCTCG GACGAAGGAA AGGCGGGTTT TCCTGTTCGA GCAGATTGTT
    ATTTTCAGCG AGCTCCTGCG GAAAGGTTCC TCCACGCCGG GGTACATGTT CAAAAGGGGC
    ATAAAGATGA ATTATCTCAT CTTAGAAGAG AACATTGACA ACGACCCGTG CAAATTCGCC
    ATTCGGAGCC GCGAGACGTC GGAGCGGGTG ATCCTGCAAG CGGCCAACGC CGACATTAAA
    CAGGCCTGGG TGCAGGACAT CGGGCAAGTG CTGGAGACCC AGAGGGACTT CCTAAACGCC
    CTGCAATCGC CAATAGAATA CCAGCGGAAG GAGAGCAACG CAGCCATGAA CAAGCCTCAG
    ATTGGCCGCG GGCCGTCCCC CACCGTTAGG CCTCTCTCCT CTACGCCCGT AGGGTGTGAA
    AAAGATGTCA CCATCCCAGT GCGGAACCAG TCGCTACCAG CGCGGAAAAT ACCTACCTCT
    AACGGCAGCG CCGGGTATGA AATGTACCCA CGGGCGGAGA AATGTGACCC AAACCAGAGC
    GAGCTGAGCG GCTGCAATGG GACCAACTCC ATGGTGGTTG TACAGGATTA CTATGCACTA
    AAGGAGAATG AGATCTGCGT GTACCACGGG GAGTTGGTAC AGATTCTGGC CATCAATCAG
    CAGAACATGT TCCTGGTATT CCGGCCGTCC AATGACCATT CCCCGGCAGC GGAAGGCTGG
    ATCCCGGGAT CAGTTTTGGG CCACATTTCT AAACCTTCCG CAGAAAATAG CGACAGATCC
    ATCAAGACGT CATGCTCTTG GCACACGCTC CGCATGAGAA AACGGGACGG GACGGAGTCC
    AACGGCCCAT GTAAGAGGCA AGAGGGCCTG GGCAGCATGG CGAAAGACCC CAATAACAGT
    TCAGAGGAGT CTGAATGCGA TGACCTTGAC CCCAGTGCTA GTATGGAGAT AATGAACCCT
    AATTTCATCC AGGAAGTGGC CCCTGAGTTC CTGGTGTCCC TGGTGGATGT CATGTGTGTT
    CTTGGGGACA CAGTCACCCT ACAGTGCAAA GTGTGCGGCC GGCCGAAGCC CAGTGTCACC
    TGGAAGGGAC CCGACCAGAG CATCTTGGAT GGGAACAGCA ACACCTACTC CATGTCAACC
    AGTGAGTCTG GAGACATCAA GCTGAAGATC TGCCACGTGA TGCCCCAGGA TAGTGGCATC
    TACACGTGTG TGGCCAGTAA TGAGCTGGGG GCAACCTCGA CATCTGCCAC CATCAAAGTG
    CAAGGGGTCC CTGGGGCCCC CAGCCAGCCG CTGGCTCAGG AGAGAAGCAG CACCTCCGTC
    ATCCTCCGCT GGCTGCCACC TACCAGCACC GGCAATTGCA CTATATCCGG CTACACTGTG
    GAATACAAGG AGGAGGGTGC CCAACTATGG CAGCAGTCAG TCGCCTCTAC CCTGGATACT
    TACCTCGTCC TAGAAGACCT GTGCCCTGGG GCCCGGTACC AGTTCCGCGT GAGCGCCAGT
    AACCCCTGGG GTATAAGTCT GCCGAGTGAG CCTTCCGAGC CAGTCAGGCT TCCAGAAACT
    GATACCAATT CCAACGGATC AACAATTTCA TGGAAAGGAA ACTTTGAGCA AGTCTACACA
    GAGCTGCATG AGATTGGAAG GGGCAGGTTC TCCATAGTGA AGAAATGTGC CAAGAAACAG
    AGCAGGAAGG ACGTGGCGGC CAAATTCATC AGCAAGAAGC TAAAGAAGCG AGAGCAGGCG
    GCGCACGAGG CGACACTTCT CCAGCACCTA CAGCACCCAC AGTACATTAC CATCCATGAC
    ACCTTTGAGT CGGACGGTGC CTACATCCTC ATCCTGGACC TCATGGAAGA TGGCCGCTTG
    CTGGATTACC TCATGAAACA CGACGAGCTG ATGGAAGAGA AAGTGGCGTT CTACATGCGA
    GACATCATGG AGGCGCTGCA GTACCTGCAC AGCTGCCGCG TGGCGCATTT AGACATCAAG
    CCGGAGAATC TCCTCATAGA CCTGCGCATC CCGGTGCCCA GAGTGAAGAT CATCGACCTG
    GAGGACGCCG TGCAGATAAC GGGGCACCAT TATATCCATC AGCTGCTGGG GAACCCCGAG
    TTTGCTGCCC CCGAGCTTAT CCAAGGGAGC CCCGTCTCTC TGGGCACAGA CATCTGGAGC
    TTGGGGGTGC TGACCTACGT CATGCTGAGC GGGGTCTCCC CGTTCCTGGA CGAGAGCAGC
    GAGGAGACCT GCGCCAACAT CTGCCGGGTG GACTTCAGTT TCCCCCAGGA GTATTTCTCG
    GGGGTCAGTC AGGCTGCCCA GGACTTCATC AAGGCCACGT TGCAAGGGGA CCCTCGCTGG
    AGACCCACCA TCGCCACCTG TCTGCAGCAC CCATGGCTGC AGCCTCATAA CAACACTTAC
    TCCAAATTAC CCCTGGACAC CTCCCGCCTG GCTGGCTTCA TTGAGAGGCG GAGCCATCAA
    AACGACGCCC AGCCATTGGT CAAAACCAAG AGTCTCATCA TTAGCAGGAA CGTGGTGGGA
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa66019
    Clone ID Related Accession (Same CDS sequence) XM_031892637.1
    Accession Version XM_031892637.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kalirin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGCGAGT CGATGCTGAA TGCCAGTCTG GTTAATGCCA GTTCACTGTC GGAAGCTGAG 
    CAGTTGCAGC GAGAACACGA GCAGTTTCAG ATGGCTATTG AGTCCCTTTT CCACGCCAGC
    TCTCTGCAGA AGACTCACCA AAGTGCCCTG CAGGTCCAGC AGAAAGCAGA GGCGCTTCTA
    CAGGCCGGGC ACTACGACGC CGACGCCATC CGGGAATGCG CCGAGAAGGT GGCGATGCAC
    TGGCAGCAAC TCATGCTCAA GATGGAGGAC AGACTCAAGC TAGTGAATGC CTCTGTGGCC
    TTCTATAAAA CCTCCGAGCA GGTGTGCAGC GTTCTGGAGA GCCTGGAGCA GGAGTACCGC
    AGGGATGAGG ACTGGTGTCG GGGCCACGAT AAGCTGGGAC CCTCCACTGA GTCGGACCAC
    GTGATGCCGC TGATCAGTAA ACACCTGGAG CAGAAGGAGG CTTTCCTTAA GGCTTGTACG
    TTAGCGCGGA GGAACGCGGA AGTCTTTCTG AAGTACATTC ACCGTAACAA CGTGAGCATG
    CCCGGCGTGG CCCCCCACAC GCGCGTCCCC GAGCAGCAAG TTAAAGCCAT ACTGAGCGAG
    CTCCTGCAAA GGGAGAACCG GGTGCTGCAC TTTTGGACCC TGAAGAAGCG GCGGTTGGAC
    CAGTGCCAGC AGTACATTGT GTTTGAGAGG AGCGCCAAGC AGGCTCTGGA CTGGATTCAG
    GAAGCCGGGG AGCATTATCT CTCCACTCGC ATCTCCCCTG GGGAATGTAT TGAGGAGACT
    CAGGAGCTGC TGAAGGAATA CGAGGAATTC CGGATACCTG CCAAGCAAAC GAAGGAGAAG
    GTGCGGCTGC TGATCCAGCT GGCAGACAGC TTTGTGGAGA AGGGCCACGT TCACGCCACC
    GAGCTGAAGA AATGGGTCAC TACTGTCAAC AAACACTACC GGGAATTCGC TCTGCGTATG
    GGCAAATACC ATTGCGGCCT GCAGCGAGCC CTGGGCATCT GCACTGAGGA CACAAAGGAC
    CTGGAGTTGG ACATTGTCCC TGCGATTCTG ACTGACCCGG AGGTGAAGCT GCGCGACGCC
    GGCCATGAAC TCAACGAGGA GAAGAGAAAA TCCGCCAGGA AAAAAGAATT CATCATGGCG
    GAACTTCTAC AGACAGAGAA AGCGTATGTG CGGGACTTGC ACGAGTGTAT GGAGACCTAC
    CTGTGGGAAA TGACCAGCGG AGTGGAGGAA ATTCCGCCCG GAATAGTGAA TAAGGAGCAC
    ATCATCTTTG GGAACATTCA GGAAATATAC GACTTTCACA ATAACATCTT CCTGAAAGAG
    CTGGAGAAGT ACGAGCAGCT GCCGGAGGAC GTGGGACATT GTTTTGTCAC TTGGGCCGAC
    AAGTTCCACA TGTACGTGAC CTACTGTAAG AACAAACCCG ACTCCAGTCA GCTGATCCTG
    GAGCACGCCG GCTCCTTCTT TGACGAGATC CAGCAGCGAC ACGGCCTGGC CAACTCCATC
    TCCTCCTACC TCATCAAACC CGTCCAGCGG ATCACTAAGT ACCAGCTGCT TCTCAAGGAG
    CTTCTGACCT GCTGTGAGGA AGGGAAAGGG GAGATAAAGG ACGGGCTGGA GGTGATGCTG
    AGTGTGCCCA AGCGGGCCAA TGATGCCATG CACGTCAGCA TGCTGGAAGG ATTTGACGAA
    AACCTGGACG TTCAAGGGGA ACTTATCCTG CAAGATTCCT TCCAAGTCTG GGACCCCAAA
    TCCCTCATCC GCAAGGGGCG CGACCGGCAC CTCTTCCTCT TCGAAATCTC CCTCGTCTTC
    AGCAAAGAGA TCAAAGACTC CACCGGCCAC ACCAAATACG TCTTTAAGAA CAAACTGCTG
    ACCTCGGAAC TGGGGGTGAC GGAGCACGTA GAGGGGGACC CCTGTAAGTT TGCCCTATGG
    TCGGGGCGCA CCCCATCATC CGATAACAAG ACAGTGCTAA AGGCATCTAG TATCGAGGTG
    AAGCAGGAAT GGATCAAGAA CATCCGGGAA GTCATTCAGG AAAGGATGAT CCATTTGAAA
    GGAGCCTTGA AAGAACCCAT CCAGCCCCCC AAAACTCCAG CAAAGCAGCG CAACAACAGC
    AAAAGGGACG TACTGGAGGA CGCCGATAGC CAGGGGGACG GCAGCAGCCA ACCGGACACC
    ATCTCCATCG CCTCCAGAAC CTCCCAGAAC ACCATGGACA GCGACAAGTT ATCTGGGGGC
    TGTGAACTGA CGACGGTTCT GCAGGATTTC ATCGCTCTCC ACAACAATGA GCTGAGCATT
    CAGGTGGGGC AAACCGTGGA GCTGCTGGAG AGGCCGAGCG ACCGGCCTGG ATGGTGCTTA
    GTCCGGACCA CCGACCGGAG CCCCCCGCAG GAAGGGCTGG TACCCAGCAG CGCCCTGTGC
    ATCTCCCACT CGCGGAGCAG CGTCGAAATG GAGTGTTTCT TCCCTTCAGG CAAAGATTCA
    TTTTCTGTCT CCGCGGCGGA AAATGGGGGG AAGTCCGAGT CGGTGGCCAA TCTGCAGCCC
    CAGGCGTCCC TGAATTCTAT CCACAGCTCC CCGGGCCCCA AACGCTCCAC CAACACCCTA
    AAAAAGTGGC TGACGAGCCC TGTGAGGCGA CTGAACAGCG GGAAGGGCGA GAGCAACATC
    AAGAAGCAAA AGAAAAGAGA CGGGCGCAAC CTCGGCTCCC CCAGAGCTGG GGACGAGACC
    ACCCCACAGG GAGACAGCGC CGATGAGAAG GGCAAGAAGG GGTGGGACGA AGAGGCTGAC
    GACGAATCCC ACACTCCGCT TCCTCCGCCA ATGAAGATCT TTGATAATGA CCCCACTCCG
    GACGACAAGT CCTCCCTGCT TGTGGTCCGG CAGAGCTCAG CGGAGGTGCC TAGTGCGGCT
    GAACTGGTCA CTGCCATAGA GAATCTGGTC AGGAACAAGA TGACCATTGA CGGAGGTTCT
    TATCGGGGCG CACAGAACAT AAATGAAGGA TTTGTGAATG AGGGTTTGGC CCCCTCGCCA
    GCTCCCAGGA ACCACGAAGA GGAGCAAAAA GCCAAAGCAC TCAGGGGCAG GATGTTTGTA
    CTGAATGAGC TGGTACAGAC GGAGAAGGAC TATGTCCGGG ATCTTGGGTT TATGGTAGAG
    ACGTACATCC CCAAGATGGA GGAGAGGGGG ACCCCGGAAG ATATGAACGG AAAAGACAAG
    ATCGTATTCG GCAACATCCA CCAGATCTAC GATTGGCACA AAGACTTTTA CTTGGCAGAA
    CTAGAGAAAT GCCTATTGGA GCCGGAGAGG TTGGCGCAGT TATTTATAAA ACATGAGAGG
    CGGCTGCACA TGTATGTGGT TTATTGCCAA AACAAACCGC GCTCGGAGTT TGTCGTTGCC
    GAATATGACG CCTACTTTGA GGATCTCATG CAGGAGGTGA ATCCCAGATT TACTGTCAGT
    GATTTTCTCA TCAAACCGAT CCAAAGGATA ACTAAGTACC AACTGCTGCT AAAGGACTTC
    CTTAAGTACA GTCAGAAGGC GGGTTTGGAG TGTTCAGAAA TCGAGAAAGC CGTGGAACTA
    ATGTGCCTTG TCCCGAAACG CTGCAACGAC ATGATGAACC TGGGGCGCCT GCAGGGATTC
    GAGGGGAAGC TGACAGCCCA GGGGAAGCTG TTGCAGCAGG ACACCTTCTA TGTGATCGAG
    CAGGAATCGG GGGTCCAGTC TCGGACGAAG GAAAGGCGGG TTTTCCTGTT CGAGCAGATT
    GTTATTTTCA GCGAGCTCCT GCGGAAAGGT TCCTCCACGC CGGGGTACAT GTTCAAAAGG
    GGCATAAAGA TGAATTATCT CATCTTAGAA GAGAACATTG ACAACGACCC GTGCAAATTC
    GCCATTCGGA GCCGCGAGAC GTCGGAGCGG GTGATCCTGC AAGCGGCCAA CGCCGACATT
    AAACAGGCCT GGGTGCAGGA CATCGGGCAA GTGCTGGAGA CCCAGAGGGA CTTCCTAAAC
    GAATACCAGC GGAAGGAGAG CAACGCAGCC ATGAACAAGC CTCAGATTGG CCGCGGGCCG
    TCCCCCACCG TTAGGCCTCT CTCCTCTACG CCCGTAGGGT GTGAAAAAGA TGTCACCATC
    CCAGTGCGGA ACCAGTCGCT ACCAGCGCGG AAAATACCTA CCTCTAACGG CAGCGCCGGG
    TATGAAATGT ACCCACGGGC GGAGAAATGT GACCCAAACC AGAGCGAGCT GAGCGGCTGC
    AATGGGACCA ACTCCATGGT GGTTGTACAG GATTACTATG CACTAAAGGA GAATGAGATC
    TGCGTGTACC ACGGGGAGTT GGTACAGATT CTGGCCATCA ATCAGCAGAA CATGTTCCTG
    GTATTCCGGC CGTCCAATGA CCATTCCCCG GCAGCGGAAG GCTGGATCCC GGGATCAGTT
    TTGGGCCACA TTTCTAAACC TTCCGCAGAA AATAGCGACA GATCCATCAA GACGTCATGC
    TCTTGGCACA CGCTCCGCAT GAGAAAACGG GACGGGACGG AGTCCAACGG CCCATGTAAG
    AGGCAAGAGG GCCTGGGCAG CATGGCGAAA GACCCCAATA ACAGTTCAGA GGAGTCTGAA
    TGCGATGACC TTGACCCCAG TGCTAGTATG GAGATAATGA ACCCTAATTT CATCCAGGAA
    GTGGCCCCTG AGTTCCTGGT GTCCCTGGTG GATGTCATGT GTGTTCTTGG GGACACAGTC
    ACCCTACAGT GCAAAGTGTG CGGCCGGCCG AAGCCCAGTG TCACCTGGAA GGGACCCGAC
    CAGAGCATCT TGGATGGGAA CAGCAACACC TACTCCATGT CAACCAGTGA GTCTGGAGAC
    ATCAAGCTGA AGATCTGCCA CGTGATGCCC CAGGATAGTG GCATCTACAC GTGTGTGGCC
    AGTAATGAGC TGGGGGCAAC CTCGACATCT GCCACCATCA AAGTGCAAGG GGTCCCTGGG
    GCCCCCAGCC AGCCGCTGGC TCAGGAGAGA AGCAGCACCT CCGTCATCCT CCGCTGGCTG
    CCACCTACCA GCACCGGCAA TTGCACTATA TCCGGCTACA CTGTGGAATA CAAGGAGGAG
    GGTGCCCAAC TATGGCAGCA GTCAGTCGCC TCTACCCTGG ATACTTACCT CGTCCTAGAA
    GACCTGTGCC CTGGGGCCCG GTACCAGTTC CGCGTGAGCG CCAGTAACCC CTGGGGTATA
    AGTCTGCCGA GTGAGCCTTC CGAGCCAGTC AGGCTTCCAG AAACTGATAC CAATTCCAAC
    GGATCAACAA TTTCATGGAA AGGAAACTTT GAGCAAGTCT ACACAGAGCT GCATGAGATT
    GGAAGGGGCA GGTTCTCCAT AGTGAAGAAA TGTGCCAAGA AACAGAGCAG GAAGGACGTG
    GCGGCCAAAT TCATCAGCAA GAAGCTAAAG AAGCGAGAGC AGGCGGCGCA CGAGGCGACA
    CTTCTCCAGC ACCTACAGCA CCCACAGTAC ATTACCATCC ATGACACCTT TGAGTCGGAC
    GGTGCCTACA TCCTCATCCT GGACCTCATG GAAGATGGCC GCTTGCTGGA TTACCTCATG
    AAACACGACG AGCTGATGGA AGAGAAAGTG GCGTTCTACA TGCGAGACAT CATGGAGGCG
    CTGCAGTACC TGCACAGCTG CCGCGTGGCG CATTTAGACA TCAAGCCGGA GAATCTCCTC
    ATAGACCTGC GCATCCCGGT GCCCAGAGTG AAGATCATCG ACCTGGAGGA CGCCGTGCAG
    ATAACGGGGC ACCATTATAT CCATCAGCTG CTGGGGAACC CCGAGTTTGC TGCCCCCGAG
    CTTATCCAAG GGAGCCCCGT CTCTCTGGGC ACAGACATCT GGAGCTTGGG GGTGCTGACC
    TACGTCATGC TGAGCGGGGT CTCCCCGTTC CTGGACGAGA GCAGCGAGGA GACCTGCGCC
    AACATCTGCC GGGTGGACTT CAGTTTCCCC CAGGAGTATT TCTCGGGGGT CAGTCAGGCT
    GCCCAGGACT TCATCAAGGC CACGTTGCAA GGGGACCCTC GCTGGAGACC CACCATCGCC
    ACCTGTCTGC AGCACCCATG GCTGCAGCCT CATAACAACA CTTACTCCAA ATTACCCCTG
    GACACCTCCC GCCTGGCTGG CTTCATTGAG AGGCGGAGCC ATCAAAACGA CGCCCAGCCA
    TTGGTCAAAA CCAAGAGTCT CATCATTAGC AGGAACGTGG TGGGAACATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748497.1
    CDS19..6189
    Translation

    Target ORF information:

    RefSeq Version XM_031892637.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kalirin RhoGEF kinase (kalrn), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892637.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGCGAGT CGATGCTGAA TGCCAGTCTG GTTAATGCCA GTTCACTGTC GGAAGCTGAG 
    CAGTTGCAGC GAGAACACGA GCAGTTTCAG ATGGCTATTG AGTCCCTTTT CCACGCCAGC
    TCTCTGCAGA AGACTCACCA AAGTGCCCTG CAGGTCCAGC AGAAAGCAGA GGCGCTTCTA
    CAGGCCGGGC ACTACGACGC CGACGCCATC CGGGAATGCG CCGAGAAGGT GGCGATGCAC
    TGGCAGCAAC TCATGCTCAA GATGGAGGAC AGACTCAAGC TAGTGAATGC CTCTGTGGCC
    TTCTATAAAA CCTCCGAGCA GGTGTGCAGC GTTCTGGAGA GCCTGGAGCA GGAGTACCGC
    AGGGATGAGG ACTGGTGTCG GGGCCACGAT AAGCTGGGAC CCTCCACTGA GTCGGACCAC
    GTGATGCCGC TGATCAGTAA ACACCTGGAG CAGAAGGAGG CTTTCCTTAA GGCTTGTACG
    TTAGCGCGGA GGAACGCGGA AGTCTTTCTG AAGTACATTC ACCGTAACAA CGTGAGCATG
    CCCGGCGTGG CCCCCCACAC GCGCGTCCCC GAGCAGCAAG TTAAAGCCAT ACTGAGCGAG
    CTCCTGCAAA GGGAGAACCG GGTGCTGCAC TTTTGGACCC TGAAGAAGCG GCGGTTGGAC
    CAGTGCCAGC AGTACATTGT GTTTGAGAGG AGCGCCAAGC AGGCTCTGGA CTGGATTCAG
    GAAGCCGGGG AGCATTATCT CTCCACTCGC ATCTCCCCTG GGGAATGTAT TGAGGAGACT
    CAGGAGCTGC TGAAGGAATA CGAGGAATTC CGGATACCTG CCAAGCAAAC GAAGGAGAAG
    GTGCGGCTGC TGATCCAGCT GGCAGACAGC TTTGTGGAGA AGGGCCACGT TCACGCCACC
    GAGCTGAAGA AATGGGTCAC TACTGTCAAC AAACACTACC GGGAATTCGC TCTGCGTATG
    GGCAAATACC ATTGCGGCCT GCAGCGAGCC CTGGGCATCT GCACTGAGGA CACAAAGGAC
    CTGGAGTTGG ACATTGTCCC TGCGATTCTG ACTGACCCGG AGGTGAAGCT GCGCGACGCC
    GGCCATGAAC TCAACGAGGA GAAGAGAAAA TCCGCCAGGA AAAAAGAATT CATCATGGCG
    GAACTTCTAC AGACAGAGAA AGCGTATGTG CGGGACTTGC ACGAGTGTAT GGAGACCTAC
    CTGTGGGAAA TGACCAGCGG AGTGGAGGAA ATTCCGCCCG GAATAGTGAA TAAGGAGCAC
    ATCATCTTTG GGAACATTCA GGAAATATAC GACTTTCACA ATAACATCTT CCTGAAAGAG
    CTGGAGAAGT ACGAGCAGCT GCCGGAGGAC GTGGGACATT GTTTTGTCAC TTGGGCCGAC
    AAGTTCCACA TGTACGTGAC CTACTGTAAG AACAAACCCG ACTCCAGTCA GCTGATCCTG
    GAGCACGCCG GCTCCTTCTT TGACGAGATC CAGCAGCGAC ACGGCCTGGC CAACTCCATC
    TCCTCCTACC TCATCAAACC CGTCCAGCGG ATCACTAAGT ACCAGCTGCT TCTCAAGGAG
    CTTCTGACCT GCTGTGAGGA AGGGAAAGGG GAGATAAAGG ACGGGCTGGA GGTGATGCTG
    AGTGTGCCCA AGCGGGCCAA TGATGCCATG CACGTCAGCA TGCTGGAAGG ATTTGACGAA
    AACCTGGACG TTCAAGGGGA ACTTATCCTG CAAGATTCCT TCCAAGTCTG GGACCCCAAA
    TCCCTCATCC GCAAGGGGCG CGACCGGCAC CTCTTCCTCT TCGAAATCTC CCTCGTCTTC
    AGCAAAGAGA TCAAAGACTC CACCGGCCAC ACCAAATACG TCTTTAAGAA CAAACTGCTG
    ACCTCGGAAC TGGGGGTGAC GGAGCACGTA GAGGGGGACC CCTGTAAGTT TGCCCTATGG
    TCGGGGCGCA CCCCATCATC CGATAACAAG ACAGTGCTAA AGGCATCTAG TATCGAGGTG
    AAGCAGGAAT GGATCAAGAA CATCCGGGAA GTCATTCAGG AAAGGATGAT CCATTTGAAA
    GGAGCCTTGA AAGAACCCAT CCAGCCCCCC AAAACTCCAG CAAAGCAGCG CAACAACAGC
    AAAAGGGACG TACTGGAGGA CGCCGATAGC CAGGGGGACG GCAGCAGCCA ACCGGACACC
    ATCTCCATCG CCTCCAGAAC CTCCCAGAAC ACCATGGACA GCGACAAGTT ATCTGGGGGC
    TGTGAACTGA CGACGGTTCT GCAGGATTTC ATCGCTCTCC ACAACAATGA GCTGAGCATT
    CAGGTGGGGC AAACCGTGGA GCTGCTGGAG AGGCCGAGCG ACCGGCCTGG ATGGTGCTTA
    GTCCGGACCA CCGACCGGAG CCCCCCGCAG GAAGGGCTGG TACCCAGCAG CGCCCTGTGC
    ATCTCCCACT CGCGGAGCAG CGTCGAAATG GAGTGTTTCT TCCCTTCAGG CAAAGATTCA
    TTTTCTGTCT CCGCGGCGGA AAATGGGGGG AAGTCCGAGT CGGTGGCCAA TCTGCAGCCC
    CAGGCGTCCC TGAATTCTAT CCACAGCTCC CCGGGCCCCA AACGCTCCAC CAACACCCTA
    AAAAAGTGGC TGACGAGCCC TGTGAGGCGA CTGAACAGCG GGAAGGGCGA GAGCAACATC
    AAGAAGCAAA AGAAAAGAGA CGGGCGCAAC CTCGGCTCCC CCAGAGCTGG GGACGAGACC
    ACCCCACAGG GAGACAGCGC CGATGAGAAG GGCAAGAAGG GGTGGGACGA AGAGGCTGAC
    GACGAATCCC ACACTCCGCT TCCTCCGCCA ATGAAGATCT TTGATAATGA CCCCACTCCG
    GACGACAAGT CCTCCCTGCT TGTGGTCCGG CAGAGCTCAG CGGAGGTGCC TAGTGCGGCT
    GAACTGGTCA CTGCCATAGA GAATCTGGTC AGGAACAAGA TGACCATTGA CGGAGGTTCT
    TATCGGGGCG CACAGAACAT AAATGAAGGA TTTGTGAATG AGGGTTTGGC CCCCTCGCCA
    GCTCCCAGGA ACCACGAAGA GGAGCAAAAA GCCAAAGCAC TCAGGGGCAG GATGTTTGTA
    CTGAATGAGC TGGTACAGAC GGAGAAGGAC TATGTCCGGG ATCTTGGGTT TATGGTAGAG
    ACGTACATCC CCAAGATGGA GGAGAGGGGG ACCCCGGAAG ATATGAACGG AAAAGACAAG
    ATCGTATTCG GCAACATCCA CCAGATCTAC GATTGGCACA AAGACTTTTA CTTGGCAGAA
    CTAGAGAAAT GCCTATTGGA GCCGGAGAGG TTGGCGCAGT TATTTATAAA ACATGAGAGG
    CGGCTGCACA TGTATGTGGT TTATTGCCAA AACAAACCGC GCTCGGAGTT TGTCGTTGCC
    GAATATGACG CCTACTTTGA GGATCTCATG CAGGAGGTGA ATCCCAGATT TACTGTCAGT
    GATTTTCTCA TCAAACCGAT CCAAAGGATA ACTAAGTACC AACTGCTGCT AAAGGACTTC
    CTTAAGTACA GTCAGAAGGC GGGTTTGGAG TGTTCAGAAA TCGAGAAAGC CGTGGAACTA
    ATGTGCCTTG TCCCGAAACG CTGCAACGAC ATGATGAACC TGGGGCGCCT GCAGGGATTC
    GAGGGGAAGC TGACAGCCCA GGGGAAGCTG TTGCAGCAGG ACACCTTCTA TGTGATCGAG
    CAGGAATCGG GGGTCCAGTC TCGGACGAAG GAAAGGCGGG TTTTCCTGTT CGAGCAGATT
    GTTATTTTCA GCGAGCTCCT GCGGAAAGGT TCCTCCACGC CGGGGTACAT GTTCAAAAGG
    GGCATAAAGA TGAATTATCT CATCTTAGAA GAGAACATTG ACAACGACCC GTGCAAATTC
    GCCATTCGGA GCCGCGAGAC GTCGGAGCGG GTGATCCTGC AAGCGGCCAA CGCCGACATT
    AAACAGGCCT GGGTGCAGGA CATCGGGCAA GTGCTGGAGA CCCAGAGGGA CTTCCTAAAC
    GAATACCAGC GGAAGGAGAG CAACGCAGCC ATGAACAAGC CTCAGATTGG CCGCGGGCCG
    TCCCCCACCG TTAGGCCTCT CTCCTCTACG CCCGTAGGGT GTGAAAAAGA TGTCACCATC
    CCAGTGCGGA ACCAGTCGCT ACCAGCGCGG AAAATACCTA CCTCTAACGG CAGCGCCGGG
    TATGAAATGT ACCCACGGGC GGAGAAATGT GACCCAAACC AGAGCGAGCT GAGCGGCTGC
    AATGGGACCA ACTCCATGGT GGTTGTACAG GATTACTATG CACTAAAGGA GAATGAGATC
    TGCGTGTACC ACGGGGAGTT GGTACAGATT CTGGCCATCA ATCAGCAGAA CATGTTCCTG
    GTATTCCGGC CGTCCAATGA CCATTCCCCG GCAGCGGAAG GCTGGATCCC GGGATCAGTT
    TTGGGCCACA TTTCTAAACC TTCCGCAGAA AATAGCGACA GATCCATCAA GACGTCATGC
    TCTTGGCACA CGCTCCGCAT GAGAAAACGG GACGGGACGG AGTCCAACGG CCCATGTAAG
    AGGCAAGAGG GCCTGGGCAG CATGGCGAAA GACCCCAATA ACAGTTCAGA GGAGTCTGAA
    TGCGATGACC TTGACCCCAG TGCTAGTATG GAGATAATGA ACCCTAATTT CATCCAGGAA
    GTGGCCCCTG AGTTCCTGGT GTCCCTGGTG GATGTCATGT GTGTTCTTGG GGACACAGTC
    ACCCTACAGT GCAAAGTGTG CGGCCGGCCG AAGCCCAGTG TCACCTGGAA GGGACCCGAC
    CAGAGCATCT TGGATGGGAA CAGCAACACC TACTCCATGT CAACCAGTGA GTCTGGAGAC
    ATCAAGCTGA AGATCTGCCA CGTGATGCCC CAGGATAGTG GCATCTACAC GTGTGTGGCC
    AGTAATGAGC TGGGGGCAAC CTCGACATCT GCCACCATCA AAGTGCAAGG GGTCCCTGGG
    GCCCCCAGCC AGCCGCTGGC TCAGGAGAGA AGCAGCACCT CCGTCATCCT CCGCTGGCTG
    CCACCTACCA GCACCGGCAA TTGCACTATA TCCGGCTACA CTGTGGAATA CAAGGAGGAG
    GGTGCCCAAC TATGGCAGCA GTCAGTCGCC TCTACCCTGG ATACTTACCT CGTCCTAGAA
    GACCTGTGCC CTGGGGCCCG GTACCAGTTC CGCGTGAGCG CCAGTAACCC CTGGGGTATA
    AGTCTGCCGA GTGAGCCTTC CGAGCCAGTC AGGCTTCCAG AAACTGATAC CAATTCCAAC
    GGATCAACAA TTTCATGGAA AGGAAACTTT GAGCAAGTCT ACACAGAGCT GCATGAGATT
    GGAAGGGGCA GGTTCTCCAT AGTGAAGAAA TGTGCCAAGA AACAGAGCAG GAAGGACGTG
    GCGGCCAAAT TCATCAGCAA GAAGCTAAAG AAGCGAGAGC AGGCGGCGCA CGAGGCGACA
    CTTCTCCAGC ACCTACAGCA CCCACAGTAC ATTACCATCC ATGACACCTT TGAGTCGGAC
    GGTGCCTACA TCCTCATCCT GGACCTCATG GAAGATGGCC GCTTGCTGGA TTACCTCATG
    AAACACGACG AGCTGATGGA AGAGAAAGTG GCGTTCTACA TGCGAGACAT CATGGAGGCG
    CTGCAGTACC TGCACAGCTG CCGCGTGGCG CATTTAGACA TCAAGCCGGA GAATCTCCTC
    ATAGACCTGC GCATCCCGGT GCCCAGAGTG AAGATCATCG ACCTGGAGGA CGCCGTGCAG
    ATAACGGGGC ACCATTATAT CCATCAGCTG CTGGGGAACC CCGAGTTTGC TGCCCCCGAG
    CTTATCCAAG GGAGCCCCGT CTCTCTGGGC ACAGACATCT GGAGCTTGGG GGTGCTGACC
    TACGTCATGC TGAGCGGGGT CTCCCCGTTC CTGGACGAGA GCAGCGAGGA GACCTGCGCC
    AACATCTGCC GGGTGGACTT CAGTTTCCCC CAGGAGTATT TCTCGGGGGT CAGTCAGGCT
    GCCCAGGACT TCATCAAGGC CACGTTGCAA GGGGACCCTC GCTGGAGACC CACCATCGCC
    ACCTGTCTGC AGCACCCATG GCTGCAGCCT CATAACAACA CTTACTCCAA ATTACCCCTG
    GACACCTCCC GCCTGGCTGG CTTCATTGAG AGGCGGAGCC ATCAAAACGA CGCCCAGCCA
    TTGGTCAAAA CCAAGAGTCT CATCATTAGC AGGAACGTGG TGGGAACATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa66020
    Clone ID Related Accession (Same CDS sequence) XM_031892639.1
    Accession Version XM_031892639.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6126bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kalirin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGCGAGT CGATGCTGAA TGCCAGTCTG GTTAATGCCA GTTCACTGTC GGAAGCTGAG 
    CAGTTGCAGC GAGAACACGA GCAGTTTCAG ATGGCTATTG AGTCCCTTTT CCACGCCAGC
    TCTCTGCAGA AGACTCACCA AAGTGCCCTG CAGGTCCAGC AGAAAGCAGA GGCGCTTCTA
    CAGGCCGGGC ACTACGACGC CGACGCCATC CGGGAATGCG CCGAGAAGGT GGCGATGCAC
    TGGCAGCAAC TCATGCTCAA GATGGAGGAC AGACTCAAGC TAGTGAATGC CTCTGTGGCC
    TTCTATAAAA CCTCCGAGCA GGTGTGCAGC GTTCTGGAGA GCCTGGAGCA GGAGTACCGC
    AGGGATGAGG ACTGGTGTCG GGGCCACGAT AAGCTGGGAC CCTCCACTGA GTCGGACCAC
    GTGATGCCGC TGATCAGTAA ACACCTGGAG CAGAAGGAGG CTTTCCTTAA GGCTTGTACG
    TTAGCGCGGA GGAACGCGGA AGTCTTTCTG AAGTACATTC ACCGTAACAA CGTGAGCATG
    CCCGGCGTGG CCCCCCACAC GCGCGTCCCC GAGCAGCAAG TTAAAGCCAT ACTGAGCGAG
    CTCCTGCAAA GGGAGAACCG GGTGCTGCAC TTTTGGACCC TGAAGAAGCG GCGGTTGGAC
    CAGTGCCAGC AGTACATTGT GTTTGAGAGG AGCGCCAAGC AGGCTCTGGA CTGGATTCAG
    GAAGCCGGGG AGCATTATCT CTCCACTCGC ATCTCCCCTG GGGAATGTAT TGAGGAGACT
    CAGGAGCTGC TGAAGGAATA CGAGGAATTC CGGATACCTG CCAAGCAAAC GAAGGAGAAG
    GTGCGGCTGC TGATCCAGCT GGCAGACAGC TTTGTGGAGA AGGGCCACGT TCACGCCACC
    GAGCTGAAGA AATGGGTCAC TACTGTCAAC AAACACTACC GGGAATTCGC TCTGCGTATG
    GGCAAATACC ATTGCGGCCT GCAGCGAGCC CTGGGCATCT GCACTGAGGA CACAAAGGAC
    CTGGAGTTGG ACATTGTCCC TGCGATTCTG ACTGACCCGG AGGTGAAGCT GCGCGACGCC
    GGCCATGAAC TCAACGAGGA GAAGAGAAAA TCCGCCAGGA AAAAAGAATT CATCATGGCG
    GAACTTCTAC AGACAGAGAA AGCGTATGTG CGGGACTTGC ACGAGTGTAT GGAGACCTAC
    CTGTGGGAAA TGACCAGCGG AGTGGAGGAA ATTCCGCCCG GAATAGTGAA TAAGGAGCAC
    ATCATCTTTG GGAACATTCA GGAAATATAC GACTTTCACA ATAACATCTT CCTGAAAGAG
    CTGGAGAAGT ACGAGCAGCT GCCGGAGGAC GTGGGACATT GTTTTGTCAC TTGGGCCGAC
    AAGTTCCACA TGTACGTGAC CTACTGTAAG AACAAACCCG ACTCCAGTCA GCTGATCCTG
    GAGCACGCCG GCTCCTTCTT TGACGAGATC CAGCAGCGAC ACGGCCTGGC CAACTCCATC
    TCCTCCTACC TCATCAAACC CGTCCAGCGG ATCACTAAGT ACCAGCTGCT TCTCAAGGAG
    CTTCTGACCT GCTGTGAGGA AGGGAAAGGG GAGATAAAGG ACGGGCTGGA GGTGATGCTG
    AGTGTGCCCA AGCGGGCCAA TGATGCCATG CACGTCAGCA TGCTGGAAGG ATTTGACGAA
    AACCTGGACG TTCAAGGGGA ACTTATCCTG CAAGATTCCT TCCAAGTCTG GGACCCCAAA
    TCCCTCATCC GCAAGGGGCG CGACCGGCAC CTCTTCCTCT TCGAAATCTC CCTCGTCTTC
    AGCAAAGAGA TCAAAGACTC CACCGGCCAC ACCAAATACG TCTTTAAGAA CAAACTGCTG
    ACCTCGGAAC TGGGGGTGAC GGAGCACGTA GAGGGGGACC CCTGTAAGTT TGCCCTATGG
    TCGGGGCGCA CCCCATCATC CGATAACAAG ACAGTGCTAA AGGCATCTAG TATCGAGGTG
    AAGCAGGAAT GGATCAAGAA CATCCGGGAA GTCATTCAGG AAAGGATGAT CCATTTGAAA
    GGAGCCTTGA AAGAACCCAT CCAGCCCCCC AAAACTCCAG CAAAGCAGCG CAACAACAGC
    AAAAGGGACG TACTGGAGGA CGCCGATAGC CAGGGGGACG GCAGCAGCCA ACCGGACACC
    ATCTCCATCG CCTCCAGAAC CTCCCAGAAC ACCATGGACA GCGACAAGTT ATCTGGGGGC
    TGTGAACTGA CGACGGTTCT GCAGGATTTC ATCGCTCTCC ACAACAATGA GCTGAGCATT
    CAGGTGGGGC AAACCGTGGA GCTGCTGGAG AGGCCGAGCG ACCGGCCTGG ATGGTGCTTA
    GTCCGGACCA CCGACCGGAG CCCCCCGCAG GAAGGGCTGG TACCCAGCAG CGCCCTGTGC
    ATCTCCCACT CGCGGAGCAG CGTCGAAATG GAGTGTTTCT TCCCTTCAGG CAAAGATTCA
    TTTTCTGTCT CCGCGGCGGA AAATGGGGGG AAGTCCGAGT CGGTGGCCAA TCTGCAGCCC
    CAGGCGTCCC TGAATTCTAT CCACAGCTCC CCGGGCCCCA AACGCTCCAC CAACACCCTA
    AAAAAGTGGC TGACGAGCCC TGTGAGGCGA CTGAACAGCG GGAAGGGCGA GAGCAACATC
    AAGAAGCAAA AGAAAAGAGA CGGGCGCAAC CTCGGCTCCC CCAGAGCTGG GGACGAGACC
    ACCCCACAGG GAGACAGCGC CGATGAGAAG GGCAAGAAGG GGTGGGACGA AGAGGCTGAC
    GACGAATCCC ACACTCCGCT TCCTCCGCCA ATGAAGATCT TTGATAATGA CCCCACTCCG
    GACGACAAGT CCTCCCTGCT TGTGGTCCGG CAGAGCTCAG CGGAGGTGCC TAGTGCGGCT
    GAACTGGTCA CTGCCATAGA GAATCTGGTC AGGAACAAGA TGACCATTGA CGGAGGTTCT
    TATCGGGGCG CACAGAACAT AAATGAAGGA TTTGTGAATG AGGGTTTGGC CCCCTCGCCA
    GCTCCCAGGA ACCACGAAGA GGAGCAAAAA GCCAAAGCAC TCAGGGGCAG GATGTTTGTA
    CTGAATGAGC TGGTACAGAC GGAGAAGGAC TATGTCCGGG ATCTTGGGTT TATGGTAGAG
    ACGTACATCC CCAAGATGGA GGAGAGGGGG ACCCCGGAAG ATATGAACGG AAAAGACAAG
    ATCGTATTCG GCAACATCCA CCAGATCTAC GATTGGCACA AAGACTTTTA CTTGGCAGAA
    CTAGAGAAAT GCCTATTGGA GCCGGAGAGG TTGGCGCAGT TATTTATAAA ACATGAGAGG
    CGGCTGCACA TGTATGTGGT TTATTGCCAA AACAAACCGC GCTCGGAGTT TGTCGTTGCC
    GAATATGACG CCTACTTTGA GGATCTCATG CAGGAGGTGA ATCCCAGATT TACTGTCAGT
    GATTTTCTCA TCAAACCGAT CCAAAGGATA ACTAAGTACC AACTGCTGCT AAAGGACTTC
    CTTAAGTACA GTCAGAAGGC GGGTTTGGAG TGTTCAGAAA TCGAGAAAGC CGTGGAACTA
    ATGTGCCTTG TCCCGAAACG CTGCAACGAC ATGATGAACC TGGGGCGCCT GCAGGGATTC
    GAGGGGAAGC TGACAGCCCA GGGGAAGCTG TTGCAGCAGG ACACCTTCTA TGTGATCGAG
    CAGGAATCGG GGGTCCAGTC TCGGACGAAG GAAAGGCGGG TTTTCCTGTT CGAGCAGATT
    GTTATTTTCA GCGAGCTCCT GCGGAAAGGT TCCTCCACGC CGGGGTACAT GTTCAAAAGG
    GGCATAAAGA TGAATTATCT CATCTTAGAA GAGAACATTG ACAACGACCC GTGCAAATTC
    GCCATTCGGA GCCGCGAGAC GTCGGAGCGG GTGATCCTGC AAGCGGCCAA CGCCGACATT
    AAACAGGCCT GGGTGCAGGA CATCGGGCAA GTGCTGGAGA CCCAGAGGGA CTTCCTAAAC
    GCCCTGCAAT CGCCAATAGA ATACCAGCGG AAGGAGAGCA ACGCAGCCAT GAACAAGCCT
    CAGATTGGCC GCGGGCCGTC CCCCACCGTT AGGCCTCTCT CCTCTACGCC CGTAGGGTGT
    GAAAAAGATG TCACCATCCC AGTGCGGAAC CAGTCGCTAC CAGCGCGGAA AATACCTACC
    TCTAACGGCA GCGCCGGGTA TGAAATGTAC CCACGGGCGG AGAAATGTGA CCCAAACCAG
    AGCGAGCTGA GCGGCTGCAA TGGGACCAAC TCCATGGTGG TTGTACAGGA TTACTATGCA
    CTAAAGGAGA ATGAGATCTG CGTGTACCAC GGGGAGTTGG TACAGATTCT GGCCATCAAT
    CAGCAGAACA TGTTCCTGGT ATTCCGGCCG TCCAATGACC ATTCCCCGGC AGCGGAAGGC
    TGGATCCCGG GATCAGTTTT GGGCCACATT TCTAAACCTT CCGCAGAAAA TAGCGACAGA
    TCCATCAAGA CGTCATGCTC TTGGCACACG CTCCGCATGA GAAAACGGGA CGGGACGGAG
    TCCAACGGCC CATGTAAGAG GCAAGAGGGC CTGGGCAGCA TGGCGAAAAT AATGAACCCT
    AATTTCATCC AGGAAGTGGC CCCTGAGTTC CTGGTGTCCC TGGTGGATGT CATGTGTGTT
    CTTGGGGACA CAGTCACCCT ACAGTGCAAA GTGTGCGGCC GGCCGAAGCC CAGTGTCACC
    TGGAAGGGAC CCGACCAGAG CATCTTGGAT GGGAACAGCA ACACCTACTC CATGTCAACC
    AGTGAGTCTG GAGACATCAA GCTGAAGATC TGCCACGTGA TGCCCCAGGA TAGTGGCATC
    TACACGTGTG TGGCCAGTAA TGAGCTGGGG GCAACCTCGA CATCTGCCAC CATCAAAGTG
    CAAGGGGTCC CTGGGGCCCC CAGCCAGCCG CTGGCTCAGG AGAGAAGCAG CACCTCCGTC
    ATCCTCCGCT GGCTGCCACC TACCAGCACC GGCAATTGCA CTATATCCGG CTACACTGTG
    GAATACAAGG AGGAGGGTGC CCAACTATGG CAGCAGTCAG TCGCCTCTAC CCTGGATACT
    TACCTCGTCC TAGAAGACCT GTGCCCTGGG GCCCGGTACC AGTTCCGCGT GAGCGCCAGT
    AACCCCTGGG GTATAAGTCT GCCGAGTGAG CCTTCCGAGC CAGTCAGGCT TCCAGAAACT
    GATACCAATT CCAACGGATC AACAATTTCA TGGAAAGGAA ACTTTGAGCA AGTCTACACA
    GAGCTGCATG AGATTGGAAG GGGCAGGTTC TCCATAGTGA AGAAATGTGC CAAGAAACAG
    AGCAGGAAGG ACGTGGCGGC CAAATTCATC AGCAAGAAGC TAAAGAAGCG AGAGCAGGCG
    GCGCACGAGG CGACACTTCT CCAGCACCTA CAGCACCCAC AGTACATTAC CATCCATGAC
    ACCTTTGAGT CGGACGGTGC CTACATCCTC ATCCTGGACC TCATGGAAGA TGGCCGCTTG
    CTGGATTACC TCATGAAACA CGACGAGCTG ATGGAAGAGA AAGTGGCGTT CTACATGCGA
    GACATCATGG AGGCGCTGCA GTACCTGCAC AGCTGCCGCG TGGCGCATTT AGACATCAAG
    CCGGAGAATC TCCTCATAGA CCTGCGCATC CCGGTGCCCA GAGTGAAGAT CATCGACCTG
    GAGGACGCCG TGCAGATAAC GGGGCACCAT TATATCCATC AGCTGCTGGG GAACCCCGAG
    TTTGCTGCCC CCGAGCTTAT CCAAGGGAGC CCCGTCTCTC TGGGCACAGA CATCTGGAGC
    TTGGGGGTGC TGACCTACGT CATGCTGAGC GGGGTCTCCC CGTTCCTGGA CGAGAGCAGC
    GAGGAGACCT GCGCCAACAT CTGCCGGGTG GACTTCAGTT TCCCCCAGGA GTATTTCTCG
    GGGGTCAGTC AGGCTGCCCA GGACTTCATC AAGGCCACGT TGCAAGGGGA CCCTCGCTGG
    AGACCCACCA TCGCCACCTG TCTGCAGCAC CCATGGCTGC AGCCTCATAA CAACACTTAC
    TCCAAATTAC CCCTGGACAC CTCCCGCCTG GCTGGCTTCA TTGAGAGGCG GAGCCATCAA
    AACGACGCCC AGCCATTGGT CAAAACCAAG AGTCTCATCA TTAGCAGGAA CGTGGTGGGA
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748499.1
    CDS18..6143
    Translation

    Target ORF information:

    RefSeq Version XM_031892639.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kalirin RhoGEF kinase (kalrn), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892639.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGCGAGT CGATGCTGAA TGCCAGTCTG GTTAATGCCA GTTCACTGTC GGAAGCTGAG 
    CAGTTGCAGC GAGAACACGA GCAGTTTCAG ATGGCTATTG AGTCCCTTTT CCACGCCAGC
    TCTCTGCAGA AGACTCACCA AAGTGCCCTG CAGGTCCAGC AGAAAGCAGA GGCGCTTCTA
    CAGGCCGGGC ACTACGACGC CGACGCCATC CGGGAATGCG CCGAGAAGGT GGCGATGCAC
    TGGCAGCAAC TCATGCTCAA GATGGAGGAC AGACTCAAGC TAGTGAATGC CTCTGTGGCC
    TTCTATAAAA CCTCCGAGCA GGTGTGCAGC GTTCTGGAGA GCCTGGAGCA GGAGTACCGC
    AGGGATGAGG ACTGGTGTCG GGGCCACGAT AAGCTGGGAC CCTCCACTGA GTCGGACCAC
    GTGATGCCGC TGATCAGTAA ACACCTGGAG CAGAAGGAGG CTTTCCTTAA GGCTTGTACG
    TTAGCGCGGA GGAACGCGGA AGTCTTTCTG AAGTACATTC ACCGTAACAA CGTGAGCATG
    CCCGGCGTGG CCCCCCACAC GCGCGTCCCC GAGCAGCAAG TTAAAGCCAT ACTGAGCGAG
    CTCCTGCAAA GGGAGAACCG GGTGCTGCAC TTTTGGACCC TGAAGAAGCG GCGGTTGGAC
    CAGTGCCAGC AGTACATTGT GTTTGAGAGG AGCGCCAAGC AGGCTCTGGA CTGGATTCAG
    GAAGCCGGGG AGCATTATCT CTCCACTCGC ATCTCCCCTG GGGAATGTAT TGAGGAGACT
    CAGGAGCTGC TGAAGGAATA CGAGGAATTC CGGATACCTG CCAAGCAAAC GAAGGAGAAG
    GTGCGGCTGC TGATCCAGCT GGCAGACAGC TTTGTGGAGA AGGGCCACGT TCACGCCACC
    GAGCTGAAGA AATGGGTCAC TACTGTCAAC AAACACTACC GGGAATTCGC TCTGCGTATG
    GGCAAATACC ATTGCGGCCT GCAGCGAGCC CTGGGCATCT GCACTGAGGA CACAAAGGAC
    CTGGAGTTGG ACATTGTCCC TGCGATTCTG ACTGACCCGG AGGTGAAGCT GCGCGACGCC
    GGCCATGAAC TCAACGAGGA GAAGAGAAAA TCCGCCAGGA AAAAAGAATT CATCATGGCG
    GAACTTCTAC AGACAGAGAA AGCGTATGTG CGGGACTTGC ACGAGTGTAT GGAGACCTAC
    CTGTGGGAAA TGACCAGCGG AGTGGAGGAA ATTCCGCCCG GAATAGTGAA TAAGGAGCAC
    ATCATCTTTG GGAACATTCA GGAAATATAC GACTTTCACA ATAACATCTT CCTGAAAGAG
    CTGGAGAAGT ACGAGCAGCT GCCGGAGGAC GTGGGACATT GTTTTGTCAC TTGGGCCGAC
    AAGTTCCACA TGTACGTGAC CTACTGTAAG AACAAACCCG ACTCCAGTCA GCTGATCCTG
    GAGCACGCCG GCTCCTTCTT TGACGAGATC CAGCAGCGAC ACGGCCTGGC CAACTCCATC
    TCCTCCTACC TCATCAAACC CGTCCAGCGG ATCACTAAGT ACCAGCTGCT TCTCAAGGAG
    CTTCTGACCT GCTGTGAGGA AGGGAAAGGG GAGATAAAGG ACGGGCTGGA GGTGATGCTG
    AGTGTGCCCA AGCGGGCCAA TGATGCCATG CACGTCAGCA TGCTGGAAGG ATTTGACGAA
    AACCTGGACG TTCAAGGGGA ACTTATCCTG CAAGATTCCT TCCAAGTCTG GGACCCCAAA
    TCCCTCATCC GCAAGGGGCG CGACCGGCAC CTCTTCCTCT TCGAAATCTC CCTCGTCTTC
    AGCAAAGAGA TCAAAGACTC CACCGGCCAC ACCAAATACG TCTTTAAGAA CAAACTGCTG
    ACCTCGGAAC TGGGGGTGAC GGAGCACGTA GAGGGGGACC CCTGTAAGTT TGCCCTATGG
    TCGGGGCGCA CCCCATCATC CGATAACAAG ACAGTGCTAA AGGCATCTAG TATCGAGGTG
    AAGCAGGAAT GGATCAAGAA CATCCGGGAA GTCATTCAGG AAAGGATGAT CCATTTGAAA
    GGAGCCTTGA AAGAACCCAT CCAGCCCCCC AAAACTCCAG CAAAGCAGCG CAACAACAGC
    AAAAGGGACG TACTGGAGGA CGCCGATAGC CAGGGGGACG GCAGCAGCCA ACCGGACACC
    ATCTCCATCG CCTCCAGAAC CTCCCAGAAC ACCATGGACA GCGACAAGTT ATCTGGGGGC
    TGTGAACTGA CGACGGTTCT GCAGGATTTC ATCGCTCTCC ACAACAATGA GCTGAGCATT
    CAGGTGGGGC AAACCGTGGA GCTGCTGGAG AGGCCGAGCG ACCGGCCTGG ATGGTGCTTA
    GTCCGGACCA CCGACCGGAG CCCCCCGCAG GAAGGGCTGG TACCCAGCAG CGCCCTGTGC
    ATCTCCCACT CGCGGAGCAG CGTCGAAATG GAGTGTTTCT TCCCTTCAGG CAAAGATTCA
    TTTTCTGTCT CCGCGGCGGA AAATGGGGGG AAGTCCGAGT CGGTGGCCAA TCTGCAGCCC
    CAGGCGTCCC TGAATTCTAT CCACAGCTCC CCGGGCCCCA AACGCTCCAC CAACACCCTA
    AAAAAGTGGC TGACGAGCCC TGTGAGGCGA CTGAACAGCG GGAAGGGCGA GAGCAACATC
    AAGAAGCAAA AGAAAAGAGA CGGGCGCAAC CTCGGCTCCC CCAGAGCTGG GGACGAGACC
    ACCCCACAGG GAGACAGCGC CGATGAGAAG GGCAAGAAGG GGTGGGACGA AGAGGCTGAC
    GACGAATCCC ACACTCCGCT TCCTCCGCCA ATGAAGATCT TTGATAATGA CCCCACTCCG
    GACGACAAGT CCTCCCTGCT TGTGGTCCGG CAGAGCTCAG CGGAGGTGCC TAGTGCGGCT
    GAACTGGTCA CTGCCATAGA GAATCTGGTC AGGAACAAGA TGACCATTGA CGGAGGTTCT
    TATCGGGGCG CACAGAACAT AAATGAAGGA TTTGTGAATG AGGGTTTGGC CCCCTCGCCA
    GCTCCCAGGA ACCACGAAGA GGAGCAAAAA GCCAAAGCAC TCAGGGGCAG GATGTTTGTA
    CTGAATGAGC TGGTACAGAC GGAGAAGGAC TATGTCCGGG ATCTTGGGTT TATGGTAGAG
    ACGTACATCC CCAAGATGGA GGAGAGGGGG ACCCCGGAAG ATATGAACGG AAAAGACAAG
    ATCGTATTCG GCAACATCCA CCAGATCTAC GATTGGCACA AAGACTTTTA CTTGGCAGAA
    CTAGAGAAAT GCCTATTGGA GCCGGAGAGG TTGGCGCAGT TATTTATAAA ACATGAGAGG
    CGGCTGCACA TGTATGTGGT TTATTGCCAA AACAAACCGC GCTCGGAGTT TGTCGTTGCC
    GAATATGACG CCTACTTTGA GGATCTCATG CAGGAGGTGA ATCCCAGATT TACTGTCAGT
    GATTTTCTCA TCAAACCGAT CCAAAGGATA ACTAAGTACC AACTGCTGCT AAAGGACTTC
    CTTAAGTACA GTCAGAAGGC GGGTTTGGAG TGTTCAGAAA TCGAGAAAGC CGTGGAACTA
    ATGTGCCTTG TCCCGAAACG CTGCAACGAC ATGATGAACC TGGGGCGCCT GCAGGGATTC
    GAGGGGAAGC TGACAGCCCA GGGGAAGCTG TTGCAGCAGG ACACCTTCTA TGTGATCGAG
    CAGGAATCGG GGGTCCAGTC TCGGACGAAG GAAAGGCGGG TTTTCCTGTT CGAGCAGATT
    GTTATTTTCA GCGAGCTCCT GCGGAAAGGT TCCTCCACGC CGGGGTACAT GTTCAAAAGG
    GGCATAAAGA TGAATTATCT CATCTTAGAA GAGAACATTG ACAACGACCC GTGCAAATTC
    GCCATTCGGA GCCGCGAGAC GTCGGAGCGG GTGATCCTGC AAGCGGCCAA CGCCGACATT
    AAACAGGCCT GGGTGCAGGA CATCGGGCAA GTGCTGGAGA CCCAGAGGGA CTTCCTAAAC
    GCCCTGCAAT CGCCAATAGA ATACCAGCGG AAGGAGAGCA ACGCAGCCAT GAACAAGCCT
    CAGATTGGCC GCGGGCCGTC CCCCACCGTT AGGCCTCTCT CCTCTACGCC CGTAGGGTGT
    GAAAAAGATG TCACCATCCC AGTGCGGAAC CAGTCGCTAC CAGCGCGGAA AATACCTACC
    TCTAACGGCA GCGCCGGGTA TGAAATGTAC CCACGGGCGG AGAAATGTGA CCCAAACCAG
    AGCGAGCTGA GCGGCTGCAA TGGGACCAAC TCCATGGTGG TTGTACAGGA TTACTATGCA
    CTAAAGGAGA ATGAGATCTG CGTGTACCAC GGGGAGTTGG TACAGATTCT GGCCATCAAT
    CAGCAGAACA TGTTCCTGGT ATTCCGGCCG TCCAATGACC ATTCCCCGGC AGCGGAAGGC
    TGGATCCCGG GATCAGTTTT GGGCCACATT TCTAAACCTT CCGCAGAAAA TAGCGACAGA
    TCCATCAAGA CGTCATGCTC TTGGCACACG CTCCGCATGA GAAAACGGGA CGGGACGGAG
    TCCAACGGCC CATGTAAGAG GCAAGAGGGC CTGGGCAGCA TGGCGAAAAT AATGAACCCT
    AATTTCATCC AGGAAGTGGC CCCTGAGTTC CTGGTGTCCC TGGTGGATGT CATGTGTGTT
    CTTGGGGACA CAGTCACCCT ACAGTGCAAA GTGTGCGGCC GGCCGAAGCC CAGTGTCACC
    TGGAAGGGAC CCGACCAGAG CATCTTGGAT GGGAACAGCA ACACCTACTC CATGTCAACC
    AGTGAGTCTG GAGACATCAA GCTGAAGATC TGCCACGTGA TGCCCCAGGA TAGTGGCATC
    TACACGTGTG TGGCCAGTAA TGAGCTGGGG GCAACCTCGA CATCTGCCAC CATCAAAGTG
    CAAGGGGTCC CTGGGGCCCC CAGCCAGCCG CTGGCTCAGG AGAGAAGCAG CACCTCCGTC
    ATCCTCCGCT GGCTGCCACC TACCAGCACC GGCAATTGCA CTATATCCGG CTACACTGTG
    GAATACAAGG AGGAGGGTGC CCAACTATGG CAGCAGTCAG TCGCCTCTAC CCTGGATACT
    TACCTCGTCC TAGAAGACCT GTGCCCTGGG GCCCGGTACC AGTTCCGCGT GAGCGCCAGT
    AACCCCTGGG GTATAAGTCT GCCGAGTGAG CCTTCCGAGC CAGTCAGGCT TCCAGAAACT
    GATACCAATT CCAACGGATC AACAATTTCA TGGAAAGGAA ACTTTGAGCA AGTCTACACA
    GAGCTGCATG AGATTGGAAG GGGCAGGTTC TCCATAGTGA AGAAATGTGC CAAGAAACAG
    AGCAGGAAGG ACGTGGCGGC CAAATTCATC AGCAAGAAGC TAAAGAAGCG AGAGCAGGCG
    GCGCACGAGG CGACACTTCT CCAGCACCTA CAGCACCCAC AGTACATTAC CATCCATGAC
    ACCTTTGAGT CGGACGGTGC CTACATCCTC ATCCTGGACC TCATGGAAGA TGGCCGCTTG
    CTGGATTACC TCATGAAACA CGACGAGCTG ATGGAAGAGA AAGTGGCGTT CTACATGCGA
    GACATCATGG AGGCGCTGCA GTACCTGCAC AGCTGCCGCG TGGCGCATTT AGACATCAAG
    CCGGAGAATC TCCTCATAGA CCTGCGCATC CCGGTGCCCA GAGTGAAGAT CATCGACCTG
    GAGGACGCCG TGCAGATAAC GGGGCACCAT TATATCCATC AGCTGCTGGG GAACCCCGAG
    TTTGCTGCCC CCGAGCTTAT CCAAGGGAGC CCCGTCTCTC TGGGCACAGA CATCTGGAGC
    TTGGGGGTGC TGACCTACGT CATGCTGAGC GGGGTCTCCC CGTTCCTGGA CGAGAGCAGC
    GAGGAGACCT GCGCCAACAT CTGCCGGGTG GACTTCAGTT TCCCCCAGGA GTATTTCTCG
    GGGGTCAGTC AGGCTGCCCA GGACTTCATC AAGGCCACGT TGCAAGGGGA CCCTCGCTGG
    AGACCCACCA TCGCCACCTG TCTGCAGCAC CCATGGCTGC AGCCTCATAA CAACACTTAC
    TCCAAATTAC CCCTGGACAC CTCCCGCCTG GCTGGCTTCA TTGAGAGGCG GAGCCATCAA
    AACGACGCCC AGCCATTGGT CAAAACCAAG AGTCTCATCA TTAGCAGGAA CGTGGTGGGA
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa66021
    Clone ID Related Accession (Same CDS sequence) XM_031892640.1
    Accession Version XM_031892640.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kalirin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGGCGAGT CGATGCTGAA TGCCAGTCTG GTTAATGCCA GTTCACTGTC GGAAGCTGAG 
    CAGTTGCAGC GAGAACACGA GCAGTTTCAG ATGGCTATTG AGTCCCTTTT CCACGCCAGC
    TCTCTGCAGA AGACTCACCA AAGTGCCCTG CAGGTCCAGC AGAAAGCAGA GGCGCTTCTA
    CAGGCCGGGC ACTACGACGC CGACGCCATC CGGGAATGCG CCGAGAAGGT GGCGATGCAC
    TGGCAGCAAC TCATGCTCAA GATGGAGGAC AGACTCAAGC TAGTGAATGC CTCTGTGGCC
    TTCTATAAAA CCTCCGAGCA GGTGTGCAGC GTTCTGGAGA GCCTGGAGCA GGAGTACCGC
    AGGGATGAGG ACTGGTGTCG GGGCCACGAT AAGCTGGGAC CCTCCACTGA GTCGGACCAC
    GTGATGCCGC TGATCAGTAA ACACCTGGAG CAGAAGGAGG CTTTCCTTAA GGCTTGTACG
    TTAGCGCGGA GGAACGCGGA AGTCTTTCTG AAGTACATTC ACCGTAACAA CGTGAGCATG
    CCCGGCGTGG CCCCCCACAC GCGCGTCCCC GAGCAGCAAG TTAAAGCCAT ACTGAGCGAG
    CTCCTGCAAA GGGAGAACCG GGTGCTGCAC TTTTGGACCC TGAAGAAGCG GCGGTTGGAC
    CAGTGCCAGC AGTACATTGT GTTTGAGAGG AGCGCCAAGC AGGCTCTGGA CTGGATTCAG
    GAAGCCGGGG AGCATTATCT CTCCACTCGC ATCTCCCCTG GGGAATGTAT TGAGGAGACT
    CAGGAGCTGC TGAAGGAATA CGAGGAATTC CGGATACCTG CCAAGCAAAC GAAGGAGAAG
    GTGCGGCTGC TGATCCAGCT GGCAGACAGC TTTGTGGAGA AGGGCCACGT TCACGCCACC
    GAGCTGAAGA AATGGGTCAC TACTGTCAAC AAACACTACC GGGAATTCGC TCTGCGTATG
    GGCAAATACC ATTGCGGCCT GCAGCGAGCC CTGGGCATCT GCACTGAGGA CACAAAGGAC
    CTGGAGTTGG ACATTGTCCC TGCGATTCTG ACTGACCCGG AGGTGAAGCT GCGCGACGCC
    GGCCATGAAC TCAACGAGGA GAAGAGAAAA TCCGCCAGGA AAAAAGAATT CATCATGGCG
    GAACTTCTAC AGACAGAGAA AGCGTATGTG CGGGACTTGC ACGAGTGTAT GGAGACCTAC
    CTGTGGGAAA TGACCAGCGG AGTGGAGGAA ATTCCGCCCG GAATAGTGAA TAAGGAGCAC
    ATCATCTTTG GGAACATTCA GGAAATATAC GACTTTCACA ATAACATCTT CCTGAAAGAG
    CTGGAGAAGT ACGAGCAGCT GCCGGAGGAC GTGGGACATT GTTTTGTCAC TTGGGCCGAC
    AAGTTCCACA TGTACGTGAC CTACTGTAAG AACAAACCCG ACTCCAGTCA GCTGATCCTG
    GAGCACGCCG GCTCCTTCTT TGACGAGATC CAGCAGCGAC ACGGCCTGGC CAACTCCATC
    TCCTCCTACC TCATCAAACC CGTCCAGCGG ATCACTAAGT ACCAGCTGCT TCTCAAGGAG
    CTTCTGACCT GCTGTGAGGA AGGGAAAGGG GAGATAAAGG ACGGGCTGGA GGTGATGCTG
    AGTGTGCCCA AGCGGGCCAA TGATGCCATG CACGTCAGCA TGCTGGAAGG ATTTGACGAA
    AACCTGGACG TTCAAGGGGA ACTTATCCTG CAAGATTCCT TCCAAGTCTG GGACCCCAAA
    TCCCTCATCC GCAAGGGGCG CGACCGGCAC CTCTTCCTCT TCGAAATCTC CCTCGTCTTC
    AGCAAAGAGA TCAAAGACTC CACCGGCCAC ACCAAATACG TCTTTAAGAA CAAACTGCTG
    ACCTCGGAAC TGGGGGTGAC GGAGCACGTA GAGGGGGACC CCTGTAAGTT TGCCCTATGG
    TCGGGGCGCA CCCCATCATC CGATAACAAG ACAGTGCTAA AGGCATCTAG TATCGAGGTG
    AAGCAGGAAT GGATCAAGAA CATCCGGGAA GTCATTCAGG AAAGGATGAT CCATTTGAAA
    GGAGCCTTGA AAGAACCCAT CCAGCCCCCC AAAACTCCAG CAAAGCAGCG CAACAACAGC
    AAAAGGGACG TACTGGAGGA CGCCGATAGC CAGGGGGACG GCAGCAGCCA ACCGGACACC
    ATCTCCATCG CCTCCAGAAC CTCCCAGAAC ACCATGGACA GCGACAAGTT ATCTGGGGGC
    TGTGAACTGA CGACGGTTCT GCAGGATTTC ATCGCTCTCC ACAACAATGA GCTGAGCATT
    CAGGTGGGGC AAACCGTGGA GCTGCTGGAG AGGCCGAGCG ACCGGCCTGG ATGGTGCTTA
    GTCCGGACCA CCGACCGGAG CCCCCCGCAG GAAGGGCTGG TACCCAGCAG CGCCCTGTGC
    ATCTCCCACT CGCGGAGCAG CGTCGAAATG GAGTGTTTCT TCCCTTCAGG CAAAGATTCA
    TTTTCTGTCT CCGCGGCGGA AAATGGGGGG AAGTCCGAGT CGGTGGCCAA TCTGCAGCCC
    CAGGCGTCCC TGAATTCTAT CCACAGCTCC CCGGGCCCCA AACGCTCCAC CAACACCCTA
    AAAAAGTGGC TGACGAGCCC TGTGAGGCGA CTGAACAGCG GGAAGGGCGA GAGCAACATC
    AAGAAGCAAA AGAAAAGAGA CGGGCGCAAC CTCGGCTCCC CCAGAGCTGG GGACGAGACC
    ACCCCACAGG GAGACAGCGC CGATGAGAAG GGCAAGAAGG GGTGGGACGA AGAGGCTGAC
    GACGAATCCC ACACTCCGCT TCCTCCGCCA ATGAAGATCT TTGATAATGA CCCCACTCCG
    GACGACAAGA CCATTGACGG AGGTTCTTAT CGGGGCGCAC AGAACATAAA TGAAGGATTT
    GTGAATGAGG GTTTGGCCCC CTCGCCAGCT CCCAGGAACC ACGAAGAGGA GCAAAAAGCC
    AAAGCACTCA GGGGCAGGAT GTTTGTACTG AATGAGCTGG TACAGACGGA GAAGGACTAT
    GTCCGGGATC TTGGGTTTAT GGTAGAGACG TACATCCCCA AGATGGAGGA GAGGGGGACC
    CCGGAAGATA TGAACGGAAA AGACAAGATC GTATTCGGCA ACATCCACCA GATCTACGAT
    TGGCACAAAG ACTTTTACTT GGCAGAACTA GAGAAATGCC TATTGGAGCC GGAGAGGTTG
    GCGCAGTTAT TTATAAAACA TGAGAGGCGG CTGCACATGT ATGTGGTTTA TTGCCAAAAC
    AAACCGCGCT CGGAGTTTGT CGTTGCCGAA TATGACGCCT ACTTTGAGGA TCTCATGCAG
    GAGGTGAATC CCAGATTTAC TGTCAGTGAT TTTCTCATCA AACCGATCCA AAGGATAACT
    AAGTACCAAC TGCTGCTAAA GGACTTCCTT AAGTACAGTC AGAAGGCGGG TTTGGAGTGT
    TCAGAAATCG AGAAAGCCGT GGAACTAATG TGCCTTGTCC CGAAACGCTG CAACGACATG
    ATGAACCTGG GGCGCCTGCA GGGATTCGAG GGGAAGCTGA CAGCCCAGGG GAAGCTGTTG
    CAGCAGGACA CCTTCTATGT GATCGAGCAG GAATCGGGGG TCCAGTCTCG GACGAAGGAA
    AGGCGGGTTT TCCTGTTCGA GCAGATTGTT ATTTTCAGCG AGCTCCTGCG GAAAGGTTCC
    TCCACGCCGG GGTACATGTT CAAAAGGGGC ATAAAGATGA ATTATCTCAT CTTAGAAGAG
    AACATTGACA ACGACCCGTG CAAATTCGCC ATTCGGAGCC GCGAGACGTC GGAGCGGGTG
    ATCCTGCAAG CGGCCAACGC CGACATTAAA CAGGCCTGGG TGCAGGACAT CGGGCAAGTG
    CTGGAGACCC AGAGGGACTT CCTAAACGCC CTGCAATCGC CAATAGAATA CCAGCGGAAG
    GAGAGCAACG CAGCCATGAA CAAGCCTCAG ATTGGCCGCG GGCCGTCCCC CACCGTTAGG
    CCTCTCTCCT CTACGCCCGT AGGGTGTGAA AAAGATGTCA CCATCCCAGT GCGGAACCAG
    TCGCTACCAG CGCGGAAAAT ACCTACCTCT AACGGCAGCG CCGGGTATGA AATGTACCCA
    CGGGCGGAGA AATGTGACCC AAACCAGAGC GAGCTGAGCG GCTGCAATGG GACCAACTCC
    ATGGTGGTTG TACAGGATTA CTATGCACTA AAGGAGAATG AGATCTGCGT GTACCACGGG
    GAGTTGGTAC AGATTCTGGC CATCAATCAG CAGAACATGT TCCTGGTATT CCGGCCGTCC
    AATGACCATT CCCCGGCAGC GGAAGGCTGG ATCCCGGGAT CAGTTTTGGG CCACATTTCT
    AAACCTTCCG CAGAAAATAG CGACAGATCC ATCAAGACGT CATGCTCTTG GCACACGCTC
    CGCATGAGAA AACGGGACGG GACGGAGTCC AACGGCCCAT GTAAGAGGCA AGAGGGCCTG
    GGCAGCATGG CGAAAGACCC CAATAACAGT TCAGAGGAGT CTGAATGCGA TGACCTTGAC
    CCCAGTGCTA GTATGGAGAT AATGAACCCT AATTTCATCC AGGAAGTGGC CCCTGAGTTC
    CTGGTGTCCC TGGTGGATGT CATGTGTGTT CTTGGGGACA CAGTCACCCT ACAGTGCAAA
    GTGTGCGGCC GGCCGAAGCC CAGTGTCACC TGGAAGGGAC CCGACCAGAG CATCTTGGAT
    GGGAACAGCA ACACCTACTC CATGTCAACC AGTGAGTCTG GAGACATCAA GCTGAAGATC
    TGCCACGTGA TGCCCCAGGA TAGTGGCATC TACACGTGTG TGGCCAGTAA TGAGCTGGGG
    GCAACCTCGA CATCTGCCAC CATCAAAGTG CAAGGGGTCC CTGGGGCCCC CAGCCAGCCG
    CTGGCTCAGG AGAGAAGCAG CACCTCCGTC ATCCTCCGCT GGCTGCCACC TACCAGCACC
    GGCAATTGCA CTATATCCGG CTACACTGTG GAATACAAGG AGGAGGGTGC CCAACTATGG
    CAGCAGTCAG TCGCCTCTAC CCTGGATACT TACCTCGTCC TAGAAGACCT GTGCCCTGGG
    GCCCGGTACC AGTTCCGCGT GAGCGCCAGT AACCCCTGGG GTATAAGTCT GCCGAGTGAG
    CCTTCCGAGC CAGTCAGGCT TCCAGAAACT GATACCAATT CCAACGGATC AACAATTTCA
    TGGAAAGGAA ACTTTGAGCA AGTCTACACA GAGCTGCATG AGATTGGAAG GGGCAGGTTC
    TCCATAGTGA AGAAATGTGC CAAGAAACAG AGCAGGAAGG ACGTGGCGGC CAAATTCATC
    AGCAAGAAGC TAAAGAAGCG AGAGCAGGCG GCGCACGAGG CGACACTTCT CCAGCACCTA
    CAGCACCCAC AGTACATTAC CATCCATGAC ACCTTTGAGT CGGACGGTGC CTACATCCTC
    ATCCTGGACC TCATGGAAGA TGGCCGCTTG CTGGATTACC TCATGAAACA CGACGAGCTG
    ATGGAAGAGA AAGTGGCGTT CTACATGCGA GACATCATGG AGGCGCTGCA GTACCTGCAC
    AGCTGCCGCG TGGCGCATTT AGACATCAAG CCGGAGAATC TCCTCATAGA CCTGCGCATC
    CCGGTGCCCA GAGTGAAGAT CATCGACCTG GAGGACGCCG TGCAGATAAC GGGGCACCAT
    TATATCCATC AGCTGCTGGG GAACCCCGAG TTTGCTGCCC CCGAGCTTAT CCAAGGGAGC
    CCCGTCTCTC TGGGCACAGA CATCTGGAGC TTGGGGGTGC TGACCTACGT CATGCTGAGC
    GGGGTCTCCC CGTTCCTGGA CGAGAGCAGC GAGGAGACCT GCGCCAACAT CTGCCGGGTG
    GACTTCAGTT TCCCCCAGGA GTATTTCTCG GGGGTCAGTC AGGCTGCCCA GGACTTCATC
    AAGGCCACGT TGCAAGGGGA CCCTCGCTGG AGACCCACCA TCGCCACCTG TCTGCAGCAC
    CCATGGCTGC AGCCTCATAA CAACACTTAC TCCAAATTAC CCCTGGACAC CTCCCGCCTG
    GCTGGCTTCA TTGAGAGGCG GAGCCATCAA AACGACGCCC AGCCATTGGT CAAAACCAAG
    AGTCTCATCA TTAGCAGGAA CGTGGTGGGA ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748500.1
    CDS18..6113
    Translation

    Target ORF information:

    RefSeq Version XM_031892640.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kalirin RhoGEF kinase (kalrn), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892640.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGGCGAGT CGATGCTGAA TGCCAGTCTG GTTAATGCCA GTTCACTGTC GGAAGCTGAG 
    CAGTTGCAGC GAGAACACGA GCAGTTTCAG ATGGCTATTG AGTCCCTTTT CCACGCCAGC
    TCTCTGCAGA AGACTCACCA AAGTGCCCTG CAGGTCCAGC AGAAAGCAGA GGCGCTTCTA
    CAGGCCGGGC ACTACGACGC CGACGCCATC CGGGAATGCG CCGAGAAGGT GGCGATGCAC
    TGGCAGCAAC TCATGCTCAA GATGGAGGAC AGACTCAAGC TAGTGAATGC CTCTGTGGCC
    TTCTATAAAA CCTCCGAGCA GGTGTGCAGC GTTCTGGAGA GCCTGGAGCA GGAGTACCGC
    AGGGATGAGG ACTGGTGTCG GGGCCACGAT AAGCTGGGAC CCTCCACTGA GTCGGACCAC
    GTGATGCCGC TGATCAGTAA ACACCTGGAG CAGAAGGAGG CTTTCCTTAA GGCTTGTACG
    TTAGCGCGGA GGAACGCGGA AGTCTTTCTG AAGTACATTC ACCGTAACAA CGTGAGCATG
    CCCGGCGTGG CCCCCCACAC GCGCGTCCCC GAGCAGCAAG TTAAAGCCAT ACTGAGCGAG
    CTCCTGCAAA GGGAGAACCG GGTGCTGCAC TTTTGGACCC TGAAGAAGCG GCGGTTGGAC
    CAGTGCCAGC AGTACATTGT GTTTGAGAGG AGCGCCAAGC AGGCTCTGGA CTGGATTCAG
    GAAGCCGGGG AGCATTATCT CTCCACTCGC ATCTCCCCTG GGGAATGTAT TGAGGAGACT
    CAGGAGCTGC TGAAGGAATA CGAGGAATTC CGGATACCTG CCAAGCAAAC GAAGGAGAAG
    GTGCGGCTGC TGATCCAGCT GGCAGACAGC TTTGTGGAGA AGGGCCACGT TCACGCCACC
    GAGCTGAAGA AATGGGTCAC TACTGTCAAC AAACACTACC GGGAATTCGC TCTGCGTATG
    GGCAAATACC ATTGCGGCCT GCAGCGAGCC CTGGGCATCT GCACTGAGGA CACAAAGGAC
    CTGGAGTTGG ACATTGTCCC TGCGATTCTG ACTGACCCGG AGGTGAAGCT GCGCGACGCC
    GGCCATGAAC TCAACGAGGA GAAGAGAAAA TCCGCCAGGA AAAAAGAATT CATCATGGCG
    GAACTTCTAC AGACAGAGAA AGCGTATGTG CGGGACTTGC ACGAGTGTAT GGAGACCTAC
    CTGTGGGAAA TGACCAGCGG AGTGGAGGAA ATTCCGCCCG GAATAGTGAA TAAGGAGCAC
    ATCATCTTTG GGAACATTCA GGAAATATAC GACTTTCACA ATAACATCTT CCTGAAAGAG
    CTGGAGAAGT ACGAGCAGCT GCCGGAGGAC GTGGGACATT GTTTTGTCAC TTGGGCCGAC
    AAGTTCCACA TGTACGTGAC CTACTGTAAG AACAAACCCG ACTCCAGTCA GCTGATCCTG
    GAGCACGCCG GCTCCTTCTT TGACGAGATC CAGCAGCGAC ACGGCCTGGC CAACTCCATC
    TCCTCCTACC TCATCAAACC CGTCCAGCGG ATCACTAAGT ACCAGCTGCT TCTCAAGGAG
    CTTCTGACCT GCTGTGAGGA AGGGAAAGGG GAGATAAAGG ACGGGCTGGA GGTGATGCTG
    AGTGTGCCCA AGCGGGCCAA TGATGCCATG CACGTCAGCA TGCTGGAAGG ATTTGACGAA
    AACCTGGACG TTCAAGGGGA ACTTATCCTG CAAGATTCCT TCCAAGTCTG GGACCCCAAA
    TCCCTCATCC GCAAGGGGCG CGACCGGCAC CTCTTCCTCT TCGAAATCTC CCTCGTCTTC
    AGCAAAGAGA TCAAAGACTC CACCGGCCAC ACCAAATACG TCTTTAAGAA CAAACTGCTG
    ACCTCGGAAC TGGGGGTGAC GGAGCACGTA GAGGGGGACC CCTGTAAGTT TGCCCTATGG
    TCGGGGCGCA CCCCATCATC CGATAACAAG ACAGTGCTAA AGGCATCTAG TATCGAGGTG
    AAGCAGGAAT GGATCAAGAA CATCCGGGAA GTCATTCAGG AAAGGATGAT CCATTTGAAA
    GGAGCCTTGA AAGAACCCAT CCAGCCCCCC AAAACTCCAG CAAAGCAGCG CAACAACAGC
    AAAAGGGACG TACTGGAGGA CGCCGATAGC CAGGGGGACG GCAGCAGCCA ACCGGACACC
    ATCTCCATCG CCTCCAGAAC CTCCCAGAAC ACCATGGACA GCGACAAGTT ATCTGGGGGC
    TGTGAACTGA CGACGGTTCT GCAGGATTTC ATCGCTCTCC ACAACAATGA GCTGAGCATT
    CAGGTGGGGC AAACCGTGGA GCTGCTGGAG AGGCCGAGCG ACCGGCCTGG ATGGTGCTTA
    GTCCGGACCA CCGACCGGAG CCCCCCGCAG GAAGGGCTGG TACCCAGCAG CGCCCTGTGC
    ATCTCCCACT CGCGGAGCAG CGTCGAAATG GAGTGTTTCT TCCCTTCAGG CAAAGATTCA
    TTTTCTGTCT CCGCGGCGGA AAATGGGGGG AAGTCCGAGT CGGTGGCCAA TCTGCAGCCC
    CAGGCGTCCC TGAATTCTAT CCACAGCTCC CCGGGCCCCA AACGCTCCAC CAACACCCTA
    AAAAAGTGGC TGACGAGCCC TGTGAGGCGA CTGAACAGCG GGAAGGGCGA GAGCAACATC
    AAGAAGCAAA AGAAAAGAGA CGGGCGCAAC CTCGGCTCCC CCAGAGCTGG GGACGAGACC
    ACCCCACAGG GAGACAGCGC CGATGAGAAG GGCAAGAAGG GGTGGGACGA AGAGGCTGAC
    GACGAATCCC ACACTCCGCT TCCTCCGCCA ATGAAGATCT TTGATAATGA CCCCACTCCG
    GACGACAAGA CCATTGACGG AGGTTCTTAT CGGGGCGCAC AGAACATAAA TGAAGGATTT
    GTGAATGAGG GTTTGGCCCC CTCGCCAGCT CCCAGGAACC ACGAAGAGGA GCAAAAAGCC
    AAAGCACTCA GGGGCAGGAT GTTTGTACTG AATGAGCTGG TACAGACGGA GAAGGACTAT
    GTCCGGGATC TTGGGTTTAT GGTAGAGACG TACATCCCCA AGATGGAGGA GAGGGGGACC
    CCGGAAGATA TGAACGGAAA AGACAAGATC GTATTCGGCA ACATCCACCA GATCTACGAT
    TGGCACAAAG ACTTTTACTT GGCAGAACTA GAGAAATGCC TATTGGAGCC GGAGAGGTTG
    GCGCAGTTAT TTATAAAACA TGAGAGGCGG CTGCACATGT ATGTGGTTTA TTGCCAAAAC
    AAACCGCGCT CGGAGTTTGT CGTTGCCGAA TATGACGCCT ACTTTGAGGA TCTCATGCAG
    GAGGTGAATC CCAGATTTAC TGTCAGTGAT TTTCTCATCA AACCGATCCA AAGGATAACT
    AAGTACCAAC TGCTGCTAAA GGACTTCCTT AAGTACAGTC AGAAGGCGGG TTTGGAGTGT
    TCAGAAATCG AGAAAGCCGT GGAACTAATG TGCCTTGTCC CGAAACGCTG CAACGACATG
    ATGAACCTGG GGCGCCTGCA GGGATTCGAG GGGAAGCTGA CAGCCCAGGG GAAGCTGTTG
    CAGCAGGACA CCTTCTATGT GATCGAGCAG GAATCGGGGG TCCAGTCTCG GACGAAGGAA
    AGGCGGGTTT TCCTGTTCGA GCAGATTGTT ATTTTCAGCG AGCTCCTGCG GAAAGGTTCC
    TCCACGCCGG GGTACATGTT CAAAAGGGGC ATAAAGATGA ATTATCTCAT CTTAGAAGAG
    AACATTGACA ACGACCCGTG CAAATTCGCC ATTCGGAGCC GCGAGACGTC GGAGCGGGTG
    ATCCTGCAAG CGGCCAACGC CGACATTAAA CAGGCCTGGG TGCAGGACAT CGGGCAAGTG
    CTGGAGACCC AGAGGGACTT CCTAAACGCC CTGCAATCGC CAATAGAATA CCAGCGGAAG
    GAGAGCAACG CAGCCATGAA CAAGCCTCAG ATTGGCCGCG GGCCGTCCCC CACCGTTAGG
    CCTCTCTCCT CTACGCCCGT AGGGTGTGAA AAAGATGTCA CCATCCCAGT GCGGAACCAG
    TCGCTACCAG CGCGGAAAAT ACCTACCTCT AACGGCAGCG CCGGGTATGA AATGTACCCA
    CGGGCGGAGA AATGTGACCC AAACCAGAGC GAGCTGAGCG GCTGCAATGG GACCAACTCC
    ATGGTGGTTG TACAGGATTA CTATGCACTA AAGGAGAATG AGATCTGCGT GTACCACGGG
    GAGTTGGTAC AGATTCTGGC CATCAATCAG CAGAACATGT TCCTGGTATT CCGGCCGTCC
    AATGACCATT CCCCGGCAGC GGAAGGCTGG ATCCCGGGAT CAGTTTTGGG CCACATTTCT
    AAACCTTCCG CAGAAAATAG CGACAGATCC ATCAAGACGT CATGCTCTTG GCACACGCTC
    CGCATGAGAA AACGGGACGG GACGGAGTCC AACGGCCCAT GTAAGAGGCA AGAGGGCCTG
    GGCAGCATGG CGAAAGACCC CAATAACAGT TCAGAGGAGT CTGAATGCGA TGACCTTGAC
    CCCAGTGCTA GTATGGAGAT AATGAACCCT AATTTCATCC AGGAAGTGGC CCCTGAGTTC
    CTGGTGTCCC TGGTGGATGT CATGTGTGTT CTTGGGGACA CAGTCACCCT ACAGTGCAAA
    GTGTGCGGCC GGCCGAAGCC CAGTGTCACC TGGAAGGGAC CCGACCAGAG CATCTTGGAT
    GGGAACAGCA ACACCTACTC CATGTCAACC AGTGAGTCTG GAGACATCAA GCTGAAGATC
    TGCCACGTGA TGCCCCAGGA TAGTGGCATC TACACGTGTG TGGCCAGTAA TGAGCTGGGG
    GCAACCTCGA CATCTGCCAC CATCAAAGTG CAAGGGGTCC CTGGGGCCCC CAGCCAGCCG
    CTGGCTCAGG AGAGAAGCAG CACCTCCGTC ATCCTCCGCT GGCTGCCACC TACCAGCACC
    GGCAATTGCA CTATATCCGG CTACACTGTG GAATACAAGG AGGAGGGTGC CCAACTATGG
    CAGCAGTCAG TCGCCTCTAC CCTGGATACT TACCTCGTCC TAGAAGACCT GTGCCCTGGG
    GCCCGGTACC AGTTCCGCGT GAGCGCCAGT AACCCCTGGG GTATAAGTCT GCCGAGTGAG
    CCTTCCGAGC CAGTCAGGCT TCCAGAAACT GATACCAATT CCAACGGATC AACAATTTCA
    TGGAAAGGAA ACTTTGAGCA AGTCTACACA GAGCTGCATG AGATTGGAAG GGGCAGGTTC
    TCCATAGTGA AGAAATGTGC CAAGAAACAG AGCAGGAAGG ACGTGGCGGC CAAATTCATC
    AGCAAGAAGC TAAAGAAGCG AGAGCAGGCG GCGCACGAGG CGACACTTCT CCAGCACCTA
    CAGCACCCAC AGTACATTAC CATCCATGAC ACCTTTGAGT CGGACGGTGC CTACATCCTC
    ATCCTGGACC TCATGGAAGA TGGCCGCTTG CTGGATTACC TCATGAAACA CGACGAGCTG
    ATGGAAGAGA AAGTGGCGTT CTACATGCGA GACATCATGG AGGCGCTGCA GTACCTGCAC
    AGCTGCCGCG TGGCGCATTT AGACATCAAG CCGGAGAATC TCCTCATAGA CCTGCGCATC
    CCGGTGCCCA GAGTGAAGAT CATCGACCTG GAGGACGCCG TGCAGATAAC GGGGCACCAT
    TATATCCATC AGCTGCTGGG GAACCCCGAG TTTGCTGCCC CCGAGCTTAT CCAAGGGAGC
    CCCGTCTCTC TGGGCACAGA CATCTGGAGC TTGGGGGTGC TGACCTACGT CATGCTGAGC
    GGGGTCTCCC CGTTCCTGGA CGAGAGCAGC GAGGAGACCT GCGCCAACAT CTGCCGGGTG
    GACTTCAGTT TCCCCCAGGA GTATTTCTCG GGGGTCAGTC AGGCTGCCCA GGACTTCATC
    AAGGCCACGT TGCAAGGGGA CCCTCGCTGG AGACCCACCA TCGCCACCTG TCTGCAGCAC
    CCATGGCTGC AGCCTCATAA CAACACTTAC TCCAAATTAC CCCTGGACAC CTCCCGCCTG
    GCTGGCTTCA TTGAGAGGCG GAGCCATCAA AACGACGCCC AGCCATTGGT CAAAACCAAG
    AGTCTCATCA TTAGCAGGAA CGTGGTGGGA ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.