sim1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	sim1
Entrez Gene ID	100486719
Full Name	single-minded family bHLH transcription factor 1
Synonyms	Xsim,Xsim1
General protein information	Preferred Names single-minded family bHLH transcription factor 1 Names single-minded homolog 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002932141.4	XP_002932187.2	single-minded homolog 1 isoform X2
XM_004914546.4	XP_004914603.1	single-minded homolog 1 isoform X3
XM_002932141.5	XP_002932187.2	single-minded homolog 1 isoform X2
XM_004914545.3	XP_004914602.1	single-minded homolog 1 isoform X1
XM_004914546.3	XP_004914603.1	single-minded homolog 1 isoform X3
XM_012963547.2	XP_012819001.1	single-minded homolog 1 isoform X3
XM_018094184.1	XP_017949673.1	single-minded homolog 1 isoform X4
XM_004914545.4	XP_004914602.1	single-minded homolog 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SIM1	NP_001074957.1
Danio rerio (zebrafish)	sim1a	NP_835740.1
Xenopus tropicalis (tropical clawed frog)	sim1	XP_004914602.1
Homo sapiens (human)	SIM1	NP_005059.2
Canis lupus familiaris (dog)	SIM1	XP_539058.2
Mus musculus (house mouse)	Sim1	NP_035506.2
Gallus gallus (chicken)	SIM1	XP_004940413.1
	SIM1	XP_001086054.1
Bos taurus (cattle)	SIM1	NP_001179901.1
Rattus norvegicus (Norway rat)	Sim1	NP_001101111.1

Gene Ontology

The following sim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa10455	XM_012963547.2 Latest version!	Xenopus tropicalis single-minded family bHLH transcription factor 1 (sim1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa10455	XM_004914546.3 Latest version!	Xenopus tropicalis single-minded family bHLH transcription factor 1 (sim1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa10453	XM_004914545.3 Latest version!	Xenopus tropicalis single-minded family bHLH transcription factor 1 (sim1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa10454	XM_002932141.4 Latest version!	Xenopus tropicalis single-minded family bHLH transcription factor 1 (sim1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa44610	XM_018094184.1 Latest version!	Xenopus tropicalis single-minded family bHLH transcription factor 1 (sim1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OXa58956	XM_004914545.4 Latest version!	Xenopus tropicalis SIM bHLH transcription factor 1 (sim1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa58957	XM_002932141.5 Latest version!	Xenopus tropicalis SIM bHLH transcription factor 1 (sim1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa58958	XM_004914546.4 Latest version!	Xenopus tropicalis SIM bHLH transcription factor 1 (sim1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sim1 ( XM_004914546.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914603.1 Xenopus tropicalis single-minded family bHLH transcription factor 1 (sim1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa10455
Clone ID Related Accession (Same CDS sequence)	XM_004914546.3 , XM_012963547.2
Accession Version	XM_004914546.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	single-minded homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914546.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACATCT CAGAAACAGC TTCTGTCCAT CTGGGCTTAT CACAGGTAGA GCTGACTGGG 
AACAGCATTT ATGAATATAT CCACCCTGCT GATCATGATG AGATGACAGC AGTTCTGACA 
GCTCACCAAC CCTACCACTC ACACTTTGTA CAGGAATATG AGATTGAACG CTCCTTCTTC 
CTACGGATGA AATGTGTTTT GGCAAAGAGG AATGCGGGGT TGACGTGTGG GGGGTATAAG 
GTGATACATT GCAGTGGGTA CCTGAAGATT CGCCAGTACA GTCTCGACAT GTCGCCATTT 
GATGGTTGCT ATCAAAATGT GGGCCTGGTG GCTGTTGGCC ATTCCCTCCC TCCCAGTGCA 
GTCACAGAGA TTAAACTTCA CAGCAATATG TTCATGTTCA GAGCCAGCTT AGATATGAAG 
CTCATATTTT TAGATTCCAG GGTAGCTGAA CTAACTGGAT ATGAGCCTCA AGATCTGATT 
GAAAAGACTT TATATCATCA TGTCCATGGC TGTGACACAT TTCATCTTCG CTGTGCACAT 
CACTTATTGC TTGTAAAAGG ACAGGTCACC ACCAAGTACT ACAGGTTCCT GGCTAAGCAT 
GGTGGCTGGG TGTGGGTACA GAGTTATGCC ACCATTGTAC ACAACAGCAG GTCCTCCAGA 
CCCCACTGTA TTGTCAGTGT GAATTATGTT CTAACGGATA CAGAATACAA AGGACTACAG 
CTATCTTTGG ATCAAGTTAC AACAAAGCCA CCATTTTCTT ATACAAGCTC AAATCCAACC 
ATAAATGACA GCAGAAAAAG CAGTAAAGCT CGACTCTCCA ACACAAAATC TAAATCCAGA 
ACATCACCCT ACCCACAGTA TTCTGGATTC CATACTGAAC GATCTGAATC AGACCATGAA 
AGTCAATGGG GCGGTAGCCC TCTTACTGAC ACAGCTTCAC CACAACTTCT TGATCCCATA 
GAAAGACCAA GCTCCCACCA ACACGATGTA TCTTGTGCAT ATAGACAGTA TCCAGACCGA 
AGTGGCCTTT GCTTTGGATT TTCCATTGAC CATTCAAGGT TAAGTGATGA TAGACACCAC 
CATAGTCAGT CGTGTGAAGG AGGCAGATGT GAGGCTGGCA GATACTTTCT TGGAAGTCCT 
CAGACTGGAA GAGAGACCTG GTGGGGTTCT CGATCTGTGT TGCCTGTTCC AAAGACCTCT 
CCAGATGGAA AAGACATATA TGAGGCTACC ATGCCACACA TTGCATCAGG TCACAGAGCA 
CATGGAAGAA GTCATTGGGA AGAAGATAGT GTTGTCAGTT CTCCTGATCC AGGCTCTGCA 
AGTGAATCAG GGGATCGTTA CCGCACTGAT CAATATCAAA ATAGTCATCA TGAACCAAGC 
AAAATTGAAA CTCTTATACG TGCTACCCAG CAGATGATTA AAGAGGAAGA AAGCAGAATA 
CAGCTGCGCA AACCTAGTCC AGATCAGCTA ACTACCATTA ATGGAGCTGG TAAAAGAAAC 
TCTGTTTGTT TTGCAAATTT TCACCAGCCA CAGTTAACTG GAGTTGTGTG CCGTGGTCCA 
CCTCTATCTA ACTCGTCTCC TTGTGACCAT GTTCAGCAAC GGGATGGAAA AATCCTTAGT 
CCTCGTGACC ACGAATATGA AAACAGCCCA AATGCTTTGT CAAGAATGAG TAGTCCAAAT 
TCTGACAGAG TTTCAAAATC AAGTGTAACC ATAGCAAAAG ATTATTTACA TGCAAATTTG 
TCCCCATCAC CAGCAATGGA CCATTCTGCA TCCCCAAATT ACTACAGTTC TCACAGGCAA 
TATTTTGACA AACATACTTA CACTTTTACT GGGTATGCTC TTGAACATCT TTATGACAGT 
GAATCTATTA GGAACTATTC ATTGGGATGT AACGGAACAC ATTTTGATGT GACTTCTCAT 
TTAAGGATGC AACAGGATTC TGCTCAAGTG CACAAGGGTA CATCAGTAAT TATTAGTAAC 
GGGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914603.1
CDS	944..2932
Translation	MYISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVLTAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCAHHLLLVKGQVTTKYYRFLAKHGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQVTTKPPFSYTSSNPTINDSRKSSKARLSNTKSKSRTSPYPQYSGFHTERSESDHESQWGGSPLTDTASPQLLDPIERPSSHQHDVSCAYRQYPDRSGLCFGFSIDHSRLSDDRHHHSQSCEGGRCEAGRYFLGSPQTGRETWWGSRSVLPVPKTSPDGKDIYEATMPHIASGHRAHGRSHWEEDSVVSSPDPGSASESGDRYRTDQYQNSHHEPSKIETLIRATQQMIKEEESRIQLRKPSPDQLTTINGAGKRNSVCFANFHQPQLTGVVCRGPPLSNSSPCDHVQQRDGKILSPRDHEYENSPNALSRMSSPNSDRVSKSSVTIAKDYLHANLSPSPAMDHSASPNYYSSHRQYFDKHTYTFTGYALEHLYDSESIRNYSLGCNGTHFDVTSHLRMQQDSAQVHKGTSVIISNGS

Target ORF information:

RefSeq Version	XM_004914546.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis single-minded family bHLH transcription factor 1 (sim1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914546.3

ORF Insert Sequence:

ATGTACATCT CAGAAACAGC TTCTGTCCAT CTGGGCTTAT CACAGGTAGA GCTGACTGGG 
AACAGCATTT ATGAATATAT CCACCCTGCT GATCATGATG AGATGACAGC AGTTCTGACA 
GCTCACCAAC CCTACCACTC ACACTTTGTA CAGGAATATG AGATTGAACG CTCCTTCTTC 
CTACGGATGA AATGTGTTTT GGCAAAGAGG AATGCGGGGT TGACGTGTGG GGGGTATAAG 
GTGATACATT GCAGTGGGTA CCTGAAGATT CGCCAGTACA GTCTCGACAT GTCGCCATTT 
GATGGTTGCT ATCAAAATGT GGGCCTGGTG GCTGTTGGCC ATTCCCTCCC TCCCAGTGCA 
GTCACAGAGA TTAAACTTCA CAGCAATATG TTCATGTTCA GAGCCAGCTT AGATATGAAG 
CTCATATTTT TAGATTCCAG GGTAGCTGAA CTAACTGGAT ATGAGCCTCA AGATCTGATT 
GAAAAGACTT TATATCATCA TGTCCATGGC TGTGACACAT TTCATCTTCG CTGTGCACAT 
CACTTATTGC TTGTAAAAGG ACAGGTCACC ACCAAGTACT ACAGGTTCCT GGCTAAGCAT 
GGTGGCTGGG TGTGGGTACA GAGTTATGCC ACCATTGTAC ACAACAGCAG GTCCTCCAGA 
CCCCACTGTA TTGTCAGTGT GAATTATGTT CTAACGGATA CAGAATACAA AGGACTACAG 
CTATCTTTGG ATCAAGTTAC AACAAAGCCA CCATTTTCTT ATACAAGCTC AAATCCAACC 
ATAAATGACA GCAGAAAAAG CAGTAAAGCT CGACTCTCCA ACACAAAATC TAAATCCAGA 
ACATCACCCT ACCCACAGTA TTCTGGATTC CATACTGAAC GATCTGAATC AGACCATGAA 
AGTCAATGGG GCGGTAGCCC TCTTACTGAC ACAGCTTCAC CACAACTTCT TGATCCCATA 
GAAAGACCAA GCTCCCACCA ACACGATGTA TCTTGTGCAT ATAGACAGTA TCCAGACCGA 
AGTGGCCTTT GCTTTGGATT TTCCATTGAC CATTCAAGGT TAAGTGATGA TAGACACCAC 
CATAGTCAGT CGTGTGAAGG AGGCAGATGT GAGGCTGGCA GATACTTTCT TGGAAGTCCT 
CAGACTGGAA GAGAGACCTG GTGGGGTTCT CGATCTGTGT TGCCTGTTCC AAAGACCTCT 
CCAGATGGAA AAGACATATA TGAGGCTACC ATGCCACACA TTGCATCAGG TCACAGAGCA 
CATGGAAGAA GTCATTGGGA AGAAGATAGT GTTGTCAGTT CTCCTGATCC AGGCTCTGCA 
AGTGAATCAG GGGATCGTTA CCGCACTGAT CAATATCAAA ATAGTCATCA TGAACCAAGC 
AAAATTGAAA CTCTTATACG TGCTACCCAG CAGATGATTA AAGAGGAAGA AAGCAGAATA 
CAGCTGCGCA AACCTAGTCC AGATCAGCTA ACTACCATTA ATGGAGCTGG TAAAAGAAAC 
TCTGTTTGTT TTGCAAATTT TCACCAGCCA CAGTTAACTG GAGTTGTGTG CCGTGGTCCA 
CCTCTATCTA ACTCGTCTCC TTGTGACCAT GTTCAGCAAC GGGATGGAAA AATCCTTAGT 
CCTCGTGACC ACGAATATGA AAACAGCCCA AATGCTTTGT CAAGAATGAG TAGTCCAAAT 
TCTGACAGAG TTTCAAAATC AAGTGTAACC ATAGCAAAAG ATTATTTACA TGCAAATTTG 
TCCCCATCAC CAGCAATGGA CCATTCTGCA TCCCCAAATT ACTACAGTTC TCACAGGCAA 
TATTTTGACA AACATACTTA CACTTTTACT GGGTATGCTC TTGAACATCT TTATGACAGT 
GAATCTATTA GGAACTATTC ATTGGGATGT AACGGAACAC ATTTTGATGT GACTTCTCAT 
TTAAGGATGC AACAGGATTC TGCTCAAGTG CACAAGGGTA CATCAGTAAT TATTAGTAAC 
GGGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sim1 ( XM_012963547.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819001.1 Xenopus tropicalis single-minded family bHLH transcription factor 1 (sim1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa10455
Clone ID Related Accession (Same CDS sequence)	XM_004914546.3 , XM_012963547.2
Accession Version	XM_012963547.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	single-minded homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963547.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACATCT CAGAAACAGC TTCTGTCCAT CTGGGCTTAT CACAGGTAGA GCTGACTGGG 
AACAGCATTT ATGAATATAT CCACCCTGCT GATCATGATG AGATGACAGC AGTTCTGACA 
GCTCACCAAC CCTACCACTC ACACTTTGTA CAGGAATATG AGATTGAACG CTCCTTCTTC 
CTACGGATGA AATGTGTTTT GGCAAAGAGG AATGCGGGGT TGACGTGTGG GGGGTATAAG 
GTGATACATT GCAGTGGGTA CCTGAAGATT CGCCAGTACA GTCTCGACAT GTCGCCATTT 
GATGGTTGCT ATCAAAATGT GGGCCTGGTG GCTGTTGGCC ATTCCCTCCC TCCCAGTGCA 
GTCACAGAGA TTAAACTTCA CAGCAATATG TTCATGTTCA GAGCCAGCTT AGATATGAAG 
CTCATATTTT TAGATTCCAG GGTAGCTGAA CTAACTGGAT ATGAGCCTCA AGATCTGATT 
GAAAAGACTT TATATCATCA TGTCCATGGC TGTGACACAT TTCATCTTCG CTGTGCACAT 
CACTTATTGC TTGTAAAAGG ACAGGTCACC ACCAAGTACT ACAGGTTCCT GGCTAAGCAT 
GGTGGCTGGG TGTGGGTACA GAGTTATGCC ACCATTGTAC ACAACAGCAG GTCCTCCAGA 
CCCCACTGTA TTGTCAGTGT GAATTATGTT CTAACGGATA CAGAATACAA AGGACTACAG 
CTATCTTTGG ATCAAGTTAC AACAAAGCCA CCATTTTCTT ATACAAGCTC AAATCCAACC 
ATAAATGACA GCAGAAAAAG CAGTAAAGCT CGACTCTCCA ACACAAAATC TAAATCCAGA 
ACATCACCCT ACCCACAGTA TTCTGGATTC CATACTGAAC GATCTGAATC AGACCATGAA 
AGTCAATGGG GCGGTAGCCC TCTTACTGAC ACAGCTTCAC CACAACTTCT TGATCCCATA 
GAAAGACCAA GCTCCCACCA ACACGATGTA TCTTGTGCAT ATAGACAGTA TCCAGACCGA 
AGTGGCCTTT GCTTTGGATT TTCCATTGAC CATTCAAGGT TAAGTGATGA TAGACACCAC 
CATAGTCAGT CGTGTGAAGG AGGCAGATGT GAGGCTGGCA GATACTTTCT TGGAAGTCCT 
CAGACTGGAA GAGAGACCTG GTGGGGTTCT CGATCTGTGT TGCCTGTTCC AAAGACCTCT 
CCAGATGGAA AAGACATATA TGAGGCTACC ATGCCACACA TTGCATCAGG TCACAGAGCA 
CATGGAAGAA GTCATTGGGA AGAAGATAGT GTTGTCAGTT CTCCTGATCC AGGCTCTGCA 
AGTGAATCAG GGGATCGTTA CCGCACTGAT CAATATCAAA ATAGTCATCA TGAACCAAGC 
AAAATTGAAA CTCTTATACG TGCTACCCAG CAGATGATTA AAGAGGAAGA AAGCAGAATA 
CAGCTGCGCA AACCTAGTCC AGATCAGCTA ACTACCATTA ATGGAGCTGG TAAAAGAAAC 
TCTGTTTGTT TTGCAAATTT TCACCAGCCA CAGTTAACTG GAGTTGTGTG CCGTGGTCCA 
CCTCTATCTA ACTCGTCTCC TTGTGACCAT GTTCAGCAAC GGGATGGAAA AATCCTTAGT 
CCTCGTGACC ACGAATATGA AAACAGCCCA AATGCTTTGT CAAGAATGAG TAGTCCAAAT 
TCTGACAGAG TTTCAAAATC AAGTGTAACC ATAGCAAAAG ATTATTTACA TGCAAATTTG 
TCCCCATCAC CAGCAATGGA CCATTCTGCA TCCCCAAATT ACTACAGTTC TCACAGGCAA 
TATTTTGACA AACATACTTA CACTTTTACT GGGTATGCTC TTGAACATCT TTATGACAGT 
GAATCTATTA GGAACTATTC ATTGGGATGT AACGGAACAC ATTTTGATGT GACTTCTCAT 
TTAAGGATGC AACAGGATTC TGCTCAAGTG CACAAGGGTA CATCAGTAAT TATTAGTAAC 
GGGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819001.1
CDS	832..2820
Translation	MYISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVLTAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCAHHLLLVKGQVTTKYYRFLAKHGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQVTTKPPFSYTSSNPTINDSRKSSKARLSNTKSKSRTSPYPQYSGFHTERSESDHESQWGGSPLTDTASPQLLDPIERPSSHQHDVSCAYRQYPDRSGLCFGFSIDHSRLSDDRHHHSQSCEGGRCEAGRYFLGSPQTGRETWWGSRSVLPVPKTSPDGKDIYEATMPHIASGHRAHGRSHWEEDSVVSSPDPGSASESGDRYRTDQYQNSHHEPSKIETLIRATQQMIKEEESRIQLRKPSPDQLTTINGAGKRNSVCFANFHQPQLTGVVCRGPPLSNSSPCDHVQQRDGKILSPRDHEYENSPNALSRMSSPNSDRVSKSSVTIAKDYLHANLSPSPAMDHSASPNYYSSHRQYFDKHTYTFTGYALEHLYDSESIRNYSLGCNGTHFDVTSHLRMQQDSAQVHKGTSVIISNGS

Target ORF information:

RefSeq Version	XM_012963547.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis single-minded family bHLH transcription factor 1 (sim1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963547.2

ORF Insert Sequence:

ATGTACATCT CAGAAACAGC TTCTGTCCAT CTGGGCTTAT CACAGGTAGA GCTGACTGGG 
AACAGCATTT ATGAATATAT CCACCCTGCT GATCATGATG AGATGACAGC AGTTCTGACA 
GCTCACCAAC CCTACCACTC ACACTTTGTA CAGGAATATG AGATTGAACG CTCCTTCTTC 
CTACGGATGA AATGTGTTTT GGCAAAGAGG AATGCGGGGT TGACGTGTGG GGGGTATAAG 
GTGATACATT GCAGTGGGTA CCTGAAGATT CGCCAGTACA GTCTCGACAT GTCGCCATTT 
GATGGTTGCT ATCAAAATGT GGGCCTGGTG GCTGTTGGCC ATTCCCTCCC TCCCAGTGCA 
GTCACAGAGA TTAAACTTCA CAGCAATATG TTCATGTTCA GAGCCAGCTT AGATATGAAG 
CTCATATTTT TAGATTCCAG GGTAGCTGAA CTAACTGGAT ATGAGCCTCA AGATCTGATT 
GAAAAGACTT TATATCATCA TGTCCATGGC TGTGACACAT TTCATCTTCG CTGTGCACAT 
CACTTATTGC TTGTAAAAGG ACAGGTCACC ACCAAGTACT ACAGGTTCCT GGCTAAGCAT 
GGTGGCTGGG TGTGGGTACA GAGTTATGCC ACCATTGTAC ACAACAGCAG GTCCTCCAGA 
CCCCACTGTA TTGTCAGTGT GAATTATGTT CTAACGGATA CAGAATACAA AGGACTACAG 
CTATCTTTGG ATCAAGTTAC AACAAAGCCA CCATTTTCTT ATACAAGCTC AAATCCAACC 
ATAAATGACA GCAGAAAAAG CAGTAAAGCT CGACTCTCCA ACACAAAATC TAAATCCAGA 
ACATCACCCT ACCCACAGTA TTCTGGATTC CATACTGAAC GATCTGAATC AGACCATGAA 
AGTCAATGGG GCGGTAGCCC TCTTACTGAC ACAGCTTCAC CACAACTTCT TGATCCCATA 
GAAAGACCAA GCTCCCACCA ACACGATGTA TCTTGTGCAT ATAGACAGTA TCCAGACCGA 
AGTGGCCTTT GCTTTGGATT TTCCATTGAC CATTCAAGGT TAAGTGATGA TAGACACCAC 
CATAGTCAGT CGTGTGAAGG AGGCAGATGT GAGGCTGGCA GATACTTTCT TGGAAGTCCT 
CAGACTGGAA GAGAGACCTG GTGGGGTTCT CGATCTGTGT TGCCTGTTCC AAAGACCTCT 
CCAGATGGAA AAGACATATA TGAGGCTACC ATGCCACACA TTGCATCAGG TCACAGAGCA 
CATGGAAGAA GTCATTGGGA AGAAGATAGT GTTGTCAGTT CTCCTGATCC AGGCTCTGCA 
AGTGAATCAG GGGATCGTTA CCGCACTGAT CAATATCAAA ATAGTCATCA TGAACCAAGC 
AAAATTGAAA CTCTTATACG TGCTACCCAG CAGATGATTA AAGAGGAAGA AAGCAGAATA 
CAGCTGCGCA AACCTAGTCC AGATCAGCTA ACTACCATTA ATGGAGCTGG TAAAAGAAAC 
TCTGTTTGTT TTGCAAATTT TCACCAGCCA CAGTTAACTG GAGTTGTGTG CCGTGGTCCA 
CCTCTATCTA ACTCGTCTCC TTGTGACCAT GTTCAGCAAC GGGATGGAAA AATCCTTAGT 
CCTCGTGACC ACGAATATGA AAACAGCCCA AATGCTTTGT CAAGAATGAG TAGTCCAAAT 
TCTGACAGAG TTTCAAAATC AAGTGTAACC ATAGCAAAAG ATTATTTACA TGCAAATTTG 
TCCCCATCAC CAGCAATGGA CCATTCTGCA TCCCCAAATT ACTACAGTTC TCACAGGCAA 
TATTTTGACA AACATACTTA CACTTTTACT GGGTATGCTC TTGAACATCT TTATGACAGT 
GAATCTATTA GGAACTATTC ATTGGGATGT AACGGAACAC ATTTTGATGT GACTTCTCAT 
TTAAGGATGC AACAGGATTC TGCTCAAGTG CACAAGGGTA CATCAGTAAT TATTAGTAAC 
GGGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sim1 ( XM_004914545.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914602.1 Xenopus tropicalis single-minded family bHLH transcription factor 1 (sim1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa10453
Clone ID Related Accession (Same CDS sequence)	XM_004914545.3
Accession Version	XM_004914545.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	single-minded homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914545.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAA AATCTAAAAA TGCAGCCCGG ACTAGACGGG AGAAGGAGAA CAGCGAATTT 
TATGAACTGG CTAAATTATT ACCCTTGCCC TCTGCTATCA CCTCCCAGCT GGACAAAGCC 
TCCATCATAC GCCTGACTAC CAGCTATTTA AAGATGAGAG TGGTCTTTCC TGAAGGACTT 
GGTGAAGCCT GGGGGCACAC TAGCAGGCCT AGCACGTTGG ATAATGTTGG CCGGGAACTG 
GGATCCCACC TTTTACAGAC CCTAGATGGA TTCATCTTTG TGGTGGCCCC CGATGGGAAA 
ATCATGTACA TCTCAGAAAC AGCTTCTGTC CATCTGGGCT TATCACAGGT AGAGCTGACT 
GGGAACAGCA TTTATGAATA TATCCACCCT GCTGATCATG ATGAGATGAC AGCAGTTCTG 
ACAGCTCACC AACCCTACCA CTCACACTTT GTACAGGAAT ATGAGATTGA ACGCTCCTTC 
TTCCTACGGA TGAAATGTGT TTTGGCAAAG AGGAATGCGG GGTTGACGTG TGGGGGGTAT 
AAGGTGATAC ATTGCAGTGG GTACCTGAAG ATTCGCCAGT ACAGTCTCGA CATGTCGCCA 
TTTGATGGTT GCTATCAAAA TGTGGGCCTG GTGGCTGTTG GCCATTCCCT CCCTCCCAGT 
GCAGTCACAG AGATTAAACT TCACAGCAAT ATGTTCATGT TCAGAGCCAG CTTAGATATG 
AAGCTCATAT TTTTAGATTC CAGGGTAGCT GAACTAACTG GATATGAGCC TCAAGATCTG 
ATTGAAAAGA CTTTATATCA TCATGTCCAT GGCTGTGACA CATTTCATCT TCGCTGTGCA 
CATCACTTAT TGCTTGTAAA AGGACAGGTC ACCACCAAGT ACTACAGGTT CCTGGCTAAG 
CATGGTGGCT GGGTGTGGGT ACAGAGTTAT GCCACCATTG TACACAACAG CAGGTCCTCC 
AGACCCCACT GTATTGTCAG TGTGAATTAT GTTCTAACGG ATACAGAATA CAAAGGACTA 
CAGCTATCTT TGGATCAAGT TACAACAAAG CCACCATTTT CTTATACAAG CTCAAATCCA 
ACCATAAATG ACAGCAGAAA AAGCAGTAAA GCTCGACTCT CCAACACAAA ATCTAAATCC 
AGAACATCAC CCTACCCACA GTATTCTGGA TTCCATACTG AACGATCTGA ATCAGACCAT 
GAAAGTCAAT GGGGCGGTAG CCCTCTTACT GACACAGCTT CACCACAACT TCTTGATCCC 
ATAGAAAGAC CAAGCTCCCA CCAACACGAT GTATCTTGTG CATATAGACA GTATCCAGAC 
CGAAGTGGCC TTTGCTTTGG ATTTTCCATT GACCATTCAA GGTTAAGTGA TGATAGACAC 
CACCATAGTC AGTCGTGTGA AGGAGGCAGA TGTGAGGCTG GCAGATACTT TCTTGGAAGT 
CCTCAGACTG GAAGAGAGAC CTGGTGGGGT TCTCGATCTG TGTTGCCTGT TCCAAAGACC 
TCTCCAGATG GAAAAGACAT ATATGAGGCT ACCATGCCAC ACATTGCATC AGGTCACAGA 
GCACATGGAA GAAGTCATTG GGAAGAAGAT AGTGTTGTCA GTTCTCCTGA TCCAGGCTCT 
GCAAGTGAAT CAGGGGATCG TTACCGCACT GATCAATATC AAAATAGTCA TCATGAACCA 
AGCAAAATTG AAACTCTTAT ACGTGCTACC CAGCAGATGA TTAAAGAGGA AGAAAGCAGA 
ATACAGCTGC GCAAACCTAG TCCAGATCAG CTAACTACCA TTAATGGAGC TGGTAAAAGA 
AACTCTGTTT GTTTTGCAAA TTTTCACCAG CCACAGTTAA CTGGAGTTGT GTGCCGTGGT 
CCACCTCTAT CTAACTCGTC TCCTTGTGAC CATGTTCAGC AACGGGATGG AAAAATCCTT 
AGTCCTCGTG ACCACGAATA TGAAAACAGC CCAAATGCTT TGTCAAGAAT GAGTAGTCCA 
AATTCTGACA GAGTTTCAAA ATCAAGTGTA ACCATAGCAA AAGATTATTT ACATGCAAAT 
TTGTCCCCAT CACCAGCAAT GGACCATTCT GCATCCCCAA ATTACTACAG TTCTCACAGG 
CAATATTTTG ACAAACATAC TTACACTTTT ACTGGGTATG CTCTTGAACA TCTTTATGAC 
AGTGAATCTA TTAGGAACTA TTCATTGGGA TGTAACGGAA CACATTTTGA TGTGACTTCT 
CATTTAAGGA TGCAACAGGA TTCTGCTCAA GTGCACAAGG GTACATCAGT AATTATTAGT 
AACGGGAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914602.1
CDS	459..2750
Translation	MKEKSKNAARTRREKENSEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRVVFPEGLGEAWGHTSRPSTLDNVGRELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVLTAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCAHHLLLVKGQVTTKYYRFLAKHGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQVTTKPPFSYTSSNPTINDSRKSSKARLSNTKSKSRTSPYPQYSGFHTERSESDHESQWGGSPLTDTASPQLLDPIERPSSHQHDVSCAYRQYPDRSGLCFGFSIDHSRLSDDRHHHSQSCEGGRCEAGRYFLGSPQTGRETWWGSRSVLPVPKTSPDGKDIYEATMPHIASGHRAHGRSHWEEDSVVSSPDPGSASESGDRYRTDQYQNSHHEPSKIETLIRATQQMIKEEESRIQLRKPSPDQLTTINGAGKRNSVCFANFHQPQLTGVVCRGPPLSNSSPCDHVQQRDGKILSPRDHEYENSPNALSRMSSPNSDRVSKSSVTIAKDYLHANLSPSPAMDHSASPNYYSSHRQYFDKHTYTFTGYALEHLYDSESIRNYSLGCNGTHFDVTSHLRMQQDSAQVHKGTSVIISNGS

Target ORF information:

RefSeq Version	XM_004914545.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis single-minded family bHLH transcription factor 1 (sim1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914545.3

ORF Insert Sequence:

ATGAAAGAAA AATCTAAAAA TGCAGCCCGG ACTAGACGGG AGAAGGAGAA CAGCGAATTT 
TATGAACTGG CTAAATTATT ACCCTTGCCC TCTGCTATCA CCTCCCAGCT GGACAAAGCC 
TCCATCATAC GCCTGACTAC CAGCTATTTA AAGATGAGAG TGGTCTTTCC TGAAGGACTT 
GGTGAAGCCT GGGGGCACAC TAGCAGGCCT AGCACGTTGG ATAATGTTGG CCGGGAACTG 
GGATCCCACC TTTTACAGAC CCTAGATGGA TTCATCTTTG TGGTGGCCCC CGATGGGAAA 
ATCATGTACA TCTCAGAAAC AGCTTCTGTC CATCTGGGCT TATCACAGGT AGAGCTGACT 
GGGAACAGCA TTTATGAATA TATCCACCCT GCTGATCATG ATGAGATGAC AGCAGTTCTG 
ACAGCTCACC AACCCTACCA CTCACACTTT GTACAGGAAT ATGAGATTGA ACGCTCCTTC 
TTCCTACGGA TGAAATGTGT TTTGGCAAAG AGGAATGCGG GGTTGACGTG TGGGGGGTAT 
AAGGTGATAC ATTGCAGTGG GTACCTGAAG ATTCGCCAGT ACAGTCTCGA CATGTCGCCA 
TTTGATGGTT GCTATCAAAA TGTGGGCCTG GTGGCTGTTG GCCATTCCCT CCCTCCCAGT 
GCAGTCACAG AGATTAAACT TCACAGCAAT ATGTTCATGT TCAGAGCCAG CTTAGATATG 
AAGCTCATAT TTTTAGATTC CAGGGTAGCT GAACTAACTG GATATGAGCC TCAAGATCTG 
ATTGAAAAGA CTTTATATCA TCATGTCCAT GGCTGTGACA CATTTCATCT TCGCTGTGCA 
CATCACTTAT TGCTTGTAAA AGGACAGGTC ACCACCAAGT ACTACAGGTT CCTGGCTAAG 
CATGGTGGCT GGGTGTGGGT ACAGAGTTAT GCCACCATTG TACACAACAG CAGGTCCTCC 
AGACCCCACT GTATTGTCAG TGTGAATTAT GTTCTAACGG ATACAGAATA CAAAGGACTA 
CAGCTATCTT TGGATCAAGT TACAACAAAG CCACCATTTT CTTATACAAG CTCAAATCCA 
ACCATAAATG ACAGCAGAAA AAGCAGTAAA GCTCGACTCT CCAACACAAA ATCTAAATCC 
AGAACATCAC CCTACCCACA GTATTCTGGA TTCCATACTG AACGATCTGA ATCAGACCAT 
GAAAGTCAAT GGGGCGGTAG CCCTCTTACT GACACAGCTT CACCACAACT TCTTGATCCC 
ATAGAAAGAC CAAGCTCCCA CCAACACGAT GTATCTTGTG CATATAGACA GTATCCAGAC 
CGAAGTGGCC TTTGCTTTGG ATTTTCCATT GACCATTCAA GGTTAAGTGA TGATAGACAC 
CACCATAGTC AGTCGTGTGA AGGAGGCAGA TGTGAGGCTG GCAGATACTT TCTTGGAAGT 
CCTCAGACTG GAAGAGAGAC CTGGTGGGGT TCTCGATCTG TGTTGCCTGT TCCAAAGACC 
TCTCCAGATG GAAAAGACAT ATATGAGGCT ACCATGCCAC ACATTGCATC AGGTCACAGA 
GCACATGGAA GAAGTCATTG GGAAGAAGAT AGTGTTGTCA GTTCTCCTGA TCCAGGCTCT 
GCAAGTGAAT CAGGGGATCG TTACCGCACT GATCAATATC AAAATAGTCA TCATGAACCA 
AGCAAAATTG AAACTCTTAT ACGTGCTACC CAGCAGATGA TTAAAGAGGA AGAAAGCAGA 
ATACAGCTGC GCAAACCTAG TCCAGATCAG CTAACTACCA TTAATGGAGC TGGTAAAAGA 
AACTCTGTTT GTTTTGCAAA TTTTCACCAG CCACAGTTAA CTGGAGTTGT GTGCCGTGGT 
CCACCTCTAT CTAACTCGTC TCCTTGTGAC CATGTTCAGC AACGGGATGG AAAAATCCTT 
AGTCCTCGTG ACCACGAATA TGAAAACAGC CCAAATGCTT TGTCAAGAAT GAGTAGTCCA 
AATTCTGACA GAGTTTCAAA ATCAAGTGTA ACCATAGCAA AAGATTATTT ACATGCAAAT 
TTGTCCCCAT CACCAGCAAT GGACCATTCT GCATCCCCAA ATTACTACAG TTCTCACAGG 
CAATATTTTG ACAAACATAC TTACACTTTT ACTGGGTATG CTCTTGAACA TCTTTATGAC 
AGTGAATCTA TTAGGAACTA TTCATTGGGA TGTAACGGAA CACATTTTGA TGTGACTTCT 
CATTTAAGGA TGCAACAGGA TTCTGCTCAA GTGCACAAGG GTACATCAGT AATTATTAGT 
AACGGGAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sim1 ( XM_002932141.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932187.2 Xenopus tropicalis single-minded family bHLH transcription factor 1 (sim1), transcript variant X2, mRNA.

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CloneID	OXa10454
Clone ID Related Accession (Same CDS sequence)	XM_002932141.4
Accession Version	XM_002932141.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	single-minded homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002932141.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGGCA GGGGCAAGCA GAGAGGTGCC GGACTTGGTG AAGCCTGGGG GCACACTAGC 
AGGCCTAGCA CGTTGGATAA TGTTGGCCGG GAACTGGGAT CCCACCTTTT ACAGACCCTA 
GATGGATTCA TCTTTGTGGT GGCCCCCGAT GGGAAAATCA TGTACATCTC AGAAACAGCT 
TCTGTCCATC TGGGCTTATC ACAGGTAGAG CTGACTGGGA ACAGCATTTA TGAATATATC 
CACCCTGCTG ATCATGATGA GATGACAGCA GTTCTGACAG CTCACCAACC CTACCACTCA 
CACTTTGTAC AGGAATATGA GATTGAACGC TCCTTCTTCC TACGGATGAA ATGTGTTTTG 
GCAAAGAGGA ATGCGGGGTT GACGTGTGGG GGGTATAAGG TGATACATTG CAGTGGGTAC 
CTGAAGATTC GCCAGTACAG TCTCGACATG TCGCCATTTG ATGGTTGCTA TCAAAATGTG 
GGCCTGGTGG CTGTTGGCCA TTCCCTCCCT CCCAGTGCAG TCACAGAGAT TAAACTTCAC 
AGCAATATGT TCATGTTCAG AGCCAGCTTA GATATGAAGC TCATATTTTT AGATTCCAGG 
GTAGCTGAAC TAACTGGATA TGAGCCTCAA GATCTGATTG AAAAGACTTT ATATCATCAT 
GTCCATGGCT GTGACACATT TCATCTTCGC TGTGCACATC ACTTATTGCT TGTAAAAGGA 
CAGGTCACCA CCAAGTACTA CAGGTTCCTG GCTAAGCATG GTGGCTGGGT GTGGGTACAG 
AGTTATGCCA CCATTGTACA CAACAGCAGG TCCTCCAGAC CCCACTGTAT TGTCAGTGTG 
AATTATGTTC TAACGGATAC AGAATACAAA GGACTACAGC TATCTTTGGA TCAAGTTACA 
ACAAAGCCAC CATTTTCTTA TACAAGCTCA AATCCAACCA TAAATGACAG CAGAAAAAGC 
AGTAAAGCTC GACTCTCCAA CACAAAATCT AAATCCAGAA CATCACCCTA CCCACAGTAT 
TCTGGATTCC ATACTGAACG ATCTGAATCA GACCATGAAA GTCAATGGGG CGGTAGCCCT 
CTTACTGACA CAGCTTCACC ACAACTTCTT GATCCCATAG AAAGACCAAG CTCCCACCAA 
CACGATGTAT CTTGTGCATA TAGACAGTAT CCAGACCGAA GTGGCCTTTG CTTTGGATTT 
TCCATTGACC ATTCAAGGTT AAGTGATGAT AGACACCACC ATAGTCAGTC GTGTGAAGGA 
GGCAGATGTG AGGCTGGCAG ATACTTTCTT GGAAGTCCTC AGACTGGAAG AGAGACCTGG 
TGGGGTTCTC GATCTGTGTT GCCTGTTCCA AAGACCTCTC CAGATGGAAA AGACATATAT 
GAGGCTACCA TGCCACACAT TGCATCAGGT CACAGAGCAC ATGGAAGAAG TCATTGGGAA 
GAAGATAGTG TTGTCAGTTC TCCTGATCCA GGCTCTGCAA GTGAATCAGG GGATCGTTAC 
CGCACTGATC AATATCAAAA TAGTCATCAT GAACCAAGCA AAATTGAAAC TCTTATACGT 
GCTACCCAGC AGATGATTAA AGAGGAAGAA AGCAGAATAC AGCTGCGCAA ACCTAGTCCA 
GATCAGCTAA CTACCATTAA TGGAGCTGGT AAAAGAAACT CTGTTTGTTT TGCAAATTTT 
CACCAGCCAC AGTTAACTGG AGTTGTGTGC CGTGGTCCAC CTCTATCTAA CTCGTCTCCT 
TGTGACCATG TTCAGCAACG GGATGGAAAA ATCCTTAGTC CTCGTGACCA CGAATATGAA 
AACAGCCCAA ATGCTTTGTC AAGAATGAGT AGTCCAAATT CTGACAGAGT TTCAAAATCA 
AGTGTAACCA TAGCAAAAGA TTATTTACAT GCAAATTTGT CCCCATCACC AGCAATGGAC 
CATTCTGCAT CCCCAAATTA CTACAGTTCT CACAGGCAAT ATTTTGACAA ACATACTTAC 
ACTTTTACTG GGTATGCTCT TGAACATCTT TATGACAGTG AATCTATTAG GAACTATTCA 
TTGGGATGTA ACGGAACACA TTTTGATGTG ACTTCTCATT TAAGGATGCA ACAGGATTCT 
GCTCAAGTGC ACAAGGGTAC ATCAGTAATT ATTAGTAACG GGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932187.2
CDS	55..2202
Translation	MRGRGKQRGAGLGEAWGHTSRPSTLDNVGRELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVLTAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCAHHLLLVKGQVTTKYYRFLAKHGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQVTTKPPFSYTSSNPTINDSRKSSKARLSNTKSKSRTSPYPQYSGFHTERSESDHESQWGGSPLTDTASPQLLDPIERPSSHQHDVSCAYRQYPDRSGLCFGFSIDHSRLSDDRHHHSQSCEGGRCEAGRYFLGSPQTGRETWWGSRSVLPVPKTSPDGKDIYEATMPHIASGHRAHGRSHWEEDSVVSSPDPGSASESGDRYRTDQYQNSHHEPSKIETLIRATQQMIKEEESRIQLRKPSPDQLTTINGAGKRNSVCFANFHQPQLTGVVCRGPPLSNSSPCDHVQQRDGKILSPRDHEYENSPNALSRMSSPNSDRVSKSSVTIAKDYLHANLSPSPAMDHSASPNYYSSHRQYFDKHTYTFTGYALEHLYDSESIRNYSLGCNGTHFDVTSHLRMQQDSAQVHKGTSVIISNGS

Target ORF information:

RefSeq Version	XM_002932141.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis single-minded family bHLH transcription factor 1 (sim1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932141.4

ORF Insert Sequence:

ATGAGGGGCA GGGGCAAGCA GAGAGGTGCC GGACTTGGTG AAGCCTGGGG GCACACTAGC 
AGGCCTAGCA CGTTGGATAA TGTTGGCCGG GAACTGGGAT CCCACCTTTT ACAGACCCTA 
GATGGATTCA TCTTTGTGGT GGCCCCCGAT GGGAAAATCA TGTACATCTC AGAAACAGCT 
TCTGTCCATC TGGGCTTATC ACAGGTAGAG CTGACTGGGA ACAGCATTTA TGAATATATC 
CACCCTGCTG ATCATGATGA GATGACAGCA GTTCTGACAG CTCACCAACC CTACCACTCA 
CACTTTGTAC AGGAATATGA GATTGAACGC TCCTTCTTCC TACGGATGAA ATGTGTTTTG 
GCAAAGAGGA ATGCGGGGTT GACGTGTGGG GGGTATAAGG TGATACATTG CAGTGGGTAC 
CTGAAGATTC GCCAGTACAG TCTCGACATG TCGCCATTTG ATGGTTGCTA TCAAAATGTG 
GGCCTGGTGG CTGTTGGCCA TTCCCTCCCT CCCAGTGCAG TCACAGAGAT TAAACTTCAC 
AGCAATATGT TCATGTTCAG AGCCAGCTTA GATATGAAGC TCATATTTTT AGATTCCAGG 
GTAGCTGAAC TAACTGGATA TGAGCCTCAA GATCTGATTG AAAAGACTTT ATATCATCAT 
GTCCATGGCT GTGACACATT TCATCTTCGC TGTGCACATC ACTTATTGCT TGTAAAAGGA 
CAGGTCACCA CCAAGTACTA CAGGTTCCTG GCTAAGCATG GTGGCTGGGT GTGGGTACAG 
AGTTATGCCA CCATTGTACA CAACAGCAGG TCCTCCAGAC CCCACTGTAT TGTCAGTGTG 
AATTATGTTC TAACGGATAC AGAATACAAA GGACTACAGC TATCTTTGGA TCAAGTTACA 
ACAAAGCCAC CATTTTCTTA TACAAGCTCA AATCCAACCA TAAATGACAG CAGAAAAAGC 
AGTAAAGCTC GACTCTCCAA CACAAAATCT AAATCCAGAA CATCACCCTA CCCACAGTAT 
TCTGGATTCC ATACTGAACG ATCTGAATCA GACCATGAAA GTCAATGGGG CGGTAGCCCT 
CTTACTGACA CAGCTTCACC ACAACTTCTT GATCCCATAG AAAGACCAAG CTCCCACCAA 
CACGATGTAT CTTGTGCATA TAGACAGTAT CCAGACCGAA GTGGCCTTTG CTTTGGATTT 
TCCATTGACC ATTCAAGGTT AAGTGATGAT AGACACCACC ATAGTCAGTC GTGTGAAGGA 
GGCAGATGTG AGGCTGGCAG ATACTTTCTT GGAAGTCCTC AGACTGGAAG AGAGACCTGG 
TGGGGTTCTC GATCTGTGTT GCCTGTTCCA AAGACCTCTC CAGATGGAAA AGACATATAT 
GAGGCTACCA TGCCACACAT TGCATCAGGT CACAGAGCAC ATGGAAGAAG TCATTGGGAA 
GAAGATAGTG TTGTCAGTTC TCCTGATCCA GGCTCTGCAA GTGAATCAGG GGATCGTTAC 
CGCACTGATC AATATCAAAA TAGTCATCAT GAACCAAGCA AAATTGAAAC TCTTATACGT 
GCTACCCAGC AGATGATTAA AGAGGAAGAA AGCAGAATAC AGCTGCGCAA ACCTAGTCCA 
GATCAGCTAA CTACCATTAA TGGAGCTGGT AAAAGAAACT CTGTTTGTTT TGCAAATTTT 
CACCAGCCAC AGTTAACTGG AGTTGTGTGC CGTGGTCCAC CTCTATCTAA CTCGTCTCCT 
TGTGACCATG TTCAGCAACG GGATGGAAAA ATCCTTAGTC CTCGTGACCA CGAATATGAA 
AACAGCCCAA ATGCTTTGTC AAGAATGAGT AGTCCAAATT CTGACAGAGT TTCAAAATCA 
AGTGTAACCA TAGCAAAAGA TTATTTACAT GCAAATTTGT CCCCATCACC AGCAATGGAC 
CATTCTGCAT CCCCAAATTA CTACAGTTCT CACAGGCAAT ATTTTGACAA ACATACTTAC 
ACTTTTACTG GGTATGCTCT TGAACATCTT TATGACAGTG AATCTATTAG GAACTATTCA 
TTGGGATGTA ACGGAACACA TTTTGATGTG ACTTCTCATT TAAGGATGCA ACAGGATTCT 
GCTCAAGTGC ACAAGGGTAC ATCAGTAATT ATTAGTAACG GGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sim1 ( XM_018094184.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949673.1 Xenopus tropicalis single-minded family bHLH transcription factor 1 (sim1), transcript variant X5, mRNA.

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CloneID	OXa44610
Clone ID Related Accession (Same CDS sequence)	XM_018094184.1
Accession Version	XM_018094184.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	single-minded homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCAG TTCTGACAGC TCACCAACCC TACCACTCAC ACTTTGTACA GGAATATGAG 
ATTGAACGCT CCTTCTTCCT ACGGATGAAA TGTGTTTTGG CAAAGAGGAA TGCGGGGTTG 
ACGTGTGGGG GGTATAAGGT GATACATTGC AGTGGGTACC TGAAGATTCG CCAGTACAGT 
CTCGACATGT CGCCATTTGA TGGTTGCTAT CAAAATGTGG GCCTGGTGGC TGTTGGCCAT 
TCCCTCCCTC CCAGTGCAGT CACAGAGATT AAACTTCACA GCAATATGTT CATGTTCAGA 
GCCAGCTTAG ATATGAAGCT CATATTTTTA GATTCCAGGG TAGCTGAACT AACTGGATAT 
GAGCCTCAAG ATCTGATTGA AAAGACTTTA TATCATCATG TCCATGGCTG TGACACATTT 
CATCTTCGCT GTGCACATCA CTTATTGCTT GTAAAAGGAC AGGTCACCAC CAAGTACTAC 
AGGTTCCTGG CTAAGCATGG TGGCTGGGTG TGGGTACAGA GTTATGCCAC CATTGTACAC 
AACAGCAGGT CCTCCAGACC CCACTGTATT GTCAGTGTGA ATTATGTTCT AACGGATACA 
GAATACAAAG GACTACAGCT ATCTTTGGAT CAAGTTACAA CAAAGCCACC ATTTTCTTAT 
ACAAGCTCAA ATCCAACCAT AAATGACAGC AGAAAAAGCA GTAAAGCTCG ACTCTCCAAC 
ACAAAATCTA AATCCAGAAC ATCACCCTAC CCACAGTATT CTGGATTCCA TACTGAACGA 
TCTGAATCAG ACCATGAAAG TCAATGGGGC GGTAGCCCTC TTACTGACAC AGCTTCACCA 
CAACTTCTTG ATCCCATAGA AAGACCAAGC TCCCACCAAC ACGATGTATC TTGTGCATAT 
AGACAGTATC CAGACCGAAG TGGCCTTTGC TTTGGATTTT CCATTGACCA TTCAAGGTTA 
AGTGATGATA GACACCACCA TAGTCAGTCG TGTGAAGGAG GCAGATGTGA GGCTGGCAGA 
TACTTTCTTG GAAGTCCTCA GACTGGAAGA GAGACCTGGT GGGGTTCTCG ATCTGTGTTG 
CCTGTTCCAA AGACCTCTCC AGATGGAAAA GACATATATG AGGCTACCAT GCCACACATT 
GCATCAGGTC ACAGAGCACA TGGAAGAAGT CATTGGGAAG AAGATAGTGT TGTCAGTTCT 
CCTGATCCAG GCTCTGCAAG TGAATCAGGG GATCGTTACC GCACTGATCA ATATCAAAAT 
AGTCATCATG AACCAAGCAA AATTGAAACT CTTATACGTG CTACCCAGCA GATGATTAAA 
GAGGAAGAAA GCAGAATACA GCTGCGCAAA CCTAGTCCAG ATCAGCTAAC TACCATTAAT 
GGAGCTGGTA AAAGAAACTC TGTTTGTTTT GCAAATTTTC ACCAGCCACA GTTAACTGGA 
GTTGTGTGCC GTGGTCCACC TCTATCTAAC TCGTCTCCTT GTGACCATGT TCAGCAACGG 
GATGGAAAAA TCCTTAGTCC TCGTGACCAC GAATATGAAA ACAGCCCAAA TGCTTTGTCA 
AGAATGAGTA GTCCAAATTC TGACAGAGTT TCAAAATCAA GTGTAACCAT AGCAAAAGAT 
TATTTACATG CAAATTTGTC CCCATCACCA GCAATGGACC ATTCTGCATC CCCAAATTAC 
TACAGTTCTC ACAGGCAATA TTTTGACAAA CATACTTACA CTTTTACTGG GTATGCTCTT 
GAACATCTTT ATGACAGTGA ATCTATTAGG AACTATTCAT TGGGATGTAA CGGAACACAT 
TTTGATGTGA CTTCTCATTT AAGGATGCAA CAGGATTCTG CTCAAGTGCA CAAGGGTACA 
TCAGTAATTA TTAGTAACGG GAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949673.1
CDS	930..2816
Translation	MTAVLTAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCAHHLLLVKGQVTTKYYRFLAKHGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQVTTKPPFSYTSSNPTINDSRKSSKARLSNTKSKSRTSPYPQYSGFHTERSESDHESQWGGSPLTDTASPQLLDPIERPSSHQHDVSCAYRQYPDRSGLCFGFSIDHSRLSDDRHHHSQSCEGGRCEAGRYFLGSPQTGRETWWGSRSVLPVPKTSPDGKDIYEATMPHIASGHRAHGRSHWEEDSVVSSPDPGSASESGDRYRTDQYQNSHHEPSKIETLIRATQQMIKEEESRIQLRKPSPDQLTTINGAGKRNSVCFANFHQPQLTGVVCRGPPLSNSSPCDHVQQRDGKILSPRDHEYENSPNALSRMSSPNSDRVSKSSVTIAKDYLHANLSPSPAMDHSASPNYYSSHRQYFDKHTYTFTGYALEHLYDSESIRNYSLGCNGTHFDVTSHLRMQQDSAQVHKGTSVIISNGS

Target ORF information:

RefSeq Version	XM_018094184.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis single-minded family bHLH transcription factor 1 (sim1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094184.1

ORF Insert Sequence:

ATGACAGCAG TTCTGACAGC TCACCAACCC TACCACTCAC ACTTTGTACA GGAATATGAG 
ATTGAACGCT CCTTCTTCCT ACGGATGAAA TGTGTTTTGG CAAAGAGGAA TGCGGGGTTG 
ACGTGTGGGG GGTATAAGGT GATACATTGC AGTGGGTACC TGAAGATTCG CCAGTACAGT 
CTCGACATGT CGCCATTTGA TGGTTGCTAT CAAAATGTGG GCCTGGTGGC TGTTGGCCAT 
TCCCTCCCTC CCAGTGCAGT CACAGAGATT AAACTTCACA GCAATATGTT CATGTTCAGA 
GCCAGCTTAG ATATGAAGCT CATATTTTTA GATTCCAGGG TAGCTGAACT AACTGGATAT 
GAGCCTCAAG ATCTGATTGA AAAGACTTTA TATCATCATG TCCATGGCTG TGACACATTT 
CATCTTCGCT GTGCACATCA CTTATTGCTT GTAAAAGGAC AGGTCACCAC CAAGTACTAC 
AGGTTCCTGG CTAAGCATGG TGGCTGGGTG TGGGTACAGA GTTATGCCAC CATTGTACAC 
AACAGCAGGT CCTCCAGACC CCACTGTATT GTCAGTGTGA ATTATGTTCT AACGGATACA 
GAATACAAAG GACTACAGCT ATCTTTGGAT CAAGTTACAA CAAAGCCACC ATTTTCTTAT 
ACAAGCTCAA ATCCAACCAT AAATGACAGC AGAAAAAGCA GTAAAGCTCG ACTCTCCAAC 
ACAAAATCTA AATCCAGAAC ATCACCCTAC CCACAGTATT CTGGATTCCA TACTGAACGA 
TCTGAATCAG ACCATGAAAG TCAATGGGGC GGTAGCCCTC TTACTGACAC AGCTTCACCA 
CAACTTCTTG ATCCCATAGA AAGACCAAGC TCCCACCAAC ACGATGTATC TTGTGCATAT 
AGACAGTATC CAGACCGAAG TGGCCTTTGC TTTGGATTTT CCATTGACCA TTCAAGGTTA 
AGTGATGATA GACACCACCA TAGTCAGTCG TGTGAAGGAG GCAGATGTGA GGCTGGCAGA 
TACTTTCTTG GAAGTCCTCA GACTGGAAGA GAGACCTGGT GGGGTTCTCG ATCTGTGTTG 
CCTGTTCCAA AGACCTCTCC AGATGGAAAA GACATATATG AGGCTACCAT GCCACACATT 
GCATCAGGTC ACAGAGCACA TGGAAGAAGT CATTGGGAAG AAGATAGTGT TGTCAGTTCT 
CCTGATCCAG GCTCTGCAAG TGAATCAGGG GATCGTTACC GCACTGATCA ATATCAAAAT 
AGTCATCATG AACCAAGCAA AATTGAAACT CTTATACGTG CTACCCAGCA GATGATTAAA 
GAGGAAGAAA GCAGAATACA GCTGCGCAAA CCTAGTCCAG ATCAGCTAAC TACCATTAAT 
GGAGCTGGTA AAAGAAACTC TGTTTGTTTT GCAAATTTTC ACCAGCCACA GTTAACTGGA 
GTTGTGTGCC GTGGTCCACC TCTATCTAAC TCGTCTCCTT GTGACCATGT TCAGCAACGG 
GATGGAAAAA TCCTTAGTCC TCGTGACCAC GAATATGAAA ACAGCCCAAA TGCTTTGTCA 
AGAATGAGTA GTCCAAATTC TGACAGAGTT TCAAAATCAA GTGTAACCAT AGCAAAAGAT 
TATTTACATG CAAATTTGTC CCCATCACCA GCAATGGACC ATTCTGCATC CCCAAATTAC 
TACAGTTCTC ACAGGCAATA TTTTGACAAA CATACTTACA CTTTTACTGG GTATGCTCTT 
GAACATCTTT ATGACAGTGA ATCTATTAGG AACTATTCAT TGGGATGTAA CGGAACACAT 
TTTGATGTGA CTTCTCATTT AAGGATGCAA CAGGATTCTG CTCAAGTGCA CAAGGGTACA 
TCAGTAATTA TTAGTAACGG GAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sim1 ( XM_004914545.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914602.1 Xenopus tropicalis SIM bHLH transcription factor 1 (sim1), transcript variant X1, mRNA.

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CloneID	OXa58956
Clone ID Related Accession (Same CDS sequence)	XM_004914545.4
Accession Version	XM_004914545.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	single-minded homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914545.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAA AATCTAAAAA TGCAGCCCGG ACTAGACGGG AGAAGGAGAA CAGCGAATTT 
TATGAACTGG CTAAATTATT ACCCTTGCCC TCTGCTATCA CCTCCCAGCT GGACAAAGCC 
TCCATCATAC GCCTGACTAC CAGCTATTTA AAGATGAGAG TGGTCTTTCC TGAAGGACTT 
GGTGAAGCCT GGGGGCACAC TAGCAGGCCT AGCACGTTGG ATAATGTTGG CCGGGAACTG 
GGATCCCACC TTTTACAGAC CCTAGATGGA TTCATCTTTG TGGTGGCCCC CGATGGGAAA 
ATCATGTACA TCTCAGAAAC AGCTTCTGTC CATCTGGGCT TATCACAGGT AGAGCTGACT 
GGGAACAGCA TTTATGAATA TATCCACCCT GCTGATCATG ATGAGATGAC AGCAGTTCTG 
ACAGCTCACC AACCCTACCA CTCACACTTT GTACAGGAAT ATGAGATCGA ACGCTCCTTC 
TTCCTACGGA TGAAATGTGT TTTGGCAAAG AGGAATGCGG GGTTGACGTG TGGGGGGTAT 
AAGGTGATAC ATTGCAGTGG GTACCTGAAG ATTCGCCAGT ACAGTCTCGA CATGTCGCCA 
TTTGATGGTT GCTATCAAAA TGTGGGCCTG GTGGCTGTTG GCCATTCCCT CCCTCCCAGT 
GCAGTCACAG AGATTAAACT TCACAGCAAT ATGTTCATGT TCAGAGCCAG CTTAGATATG 
AAGCTCATAT TTTTAGATTC CAGGGTAGCT GAACTAACTG GATATGAGCC TCAAGATCTG 
ATTGAAAAGA CTTTATATCA TCATGTCCAT GGCTGTGACA CATTTCATCT TCGCTGTGCA 
CATCACTTAT TGCTTGTAAA AGGACAGGTC ACCACCAAGT ACTACAGGTT CCTGGCTAAG 
CATGGTGGCT GGGTGTGGGT ACAGAGTTAT GCCACCATTG TACACAACAG CAGGTCCTCC 
AGACCCCACT GTATTGTCAG TGTGAATTAT GTTCTAACGG ATACAGAATA CAAAGGACTA 
CAGCTATCTT TGGATCAAGT TACAACAAAG CCACCATTTT CTTATACAAG CTCAAATCCA 
ACCATAAATG ACAGCAGAAA AAGCAGTAAA GCTCGACTCT CCAACACAAA ATCTAAATCC 
AGAACATCAC CCTACCCACA GTATTCTGGA TTCCATACTG AACGATCTGA ATCAGACCAT 
GAAAGTCAAT GGGGCGGTAG CCCTCTTACT GACACAGCTT CACCACAACT TCTTGATCCC 
ATAGAAAGAC CAAGCTCCCA CCAACACGAT GTATCTTGTG CATATAGACA GTATCCAGAC 
CGAAGTGGCC TTTGCTTTGG ATTTTCCATT GACCATTCAA GGTTAAGTGA TGATAGACAC 
CACCATAGTC AGTCGTGTGA AGGAGGCAGA TGTGAGGCTG GCAGATACTT TCTTGGAAGT 
CCTCAGACTG GAAGAGAGAC CTGGTGGGGT TCTCGATCTG TGTTGCCTGT TCCAAAGACC 
TCTCCAGATG GAAAAGACAT ATATGAGGCT ACCATGCCAC ACATTGCATC AGGTCACAGA 
GCACATGGAA GAAGTCATTG GGAAGAAGAT AGTGTTGTCA GTTCTCCTGA TCCAGGCTCT 
GCAAGTGAAT CAGGGGATCG TTACCGCACT GATCAATATC AAAATAGTCA TCATGAACCA 
AGCAAAATTG AAACTCTTAT ACGTGCTACC CAGCAGATGA TTAAAGAGGA AGAAAGCAGA 
ATACAGCTGC GCAAACCTAG TCCAGATCAG CTAACTACCA TTAATGGAGC TGGTAAAAGA 
AACTCTGTTT GTTTTGCAAA TTTTCACCAG CCACAGTTAA CTGGAGTTGT GTGCCGTGGT 
CCACCTCTAT CTAACTCGTC TCCTTGTGAC CATGTTCAGC AACGGGATGG AAAAATCCTT 
AGTCCTCGTG ACCACGAATA TGAAAACAGC CCAAATGCTT TGTCAAGAAT GAGTAGTCCA 
AATTCTGACA GAGTTTCAAA ATCAAGTGTA ACCATAGCAA AAGATTATTT ACATGCAAAT 
TTGTCCCCAT CACCAGCAAT GGACCATTCT GCATCCCCAA ATTACTACAG TTCTCACAGG 
CAATATTTTG ACAAACATAC TTACACTTTT ACTGGGTATG CTCTTGAACA TCTTTATGAC 
AGTGAATCTA TTAGGAACTA TTCATTGGGA TGTAACGGAA CACATTTTGA TGTGACTTCT 
CATTTAAGGA TGCAACAGGA TTCTGCTCAA GTGCACAAGG GTACATCAGT AATTATTAGT 
AACGGGAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914602.1
CDS	449..2740
Translation	MKEKSKNAARTRREKENSEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRVVFPEGLGEAWGHTSRPSTLDNVGRELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVLTAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCAHHLLLVKGQVTTKYYRFLAKHGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQVTTKPPFSYTSSNPTINDSRKSSKARLSNTKSKSRTSPYPQYSGFHTERSESDHESQWGGSPLTDTASPQLLDPIERPSSHQHDVSCAYRQYPDRSGLCFGFSIDHSRLSDDRHHHSQSCEGGRCEAGRYFLGSPQTGRETWWGSRSVLPVPKTSPDGKDIYEATMPHIASGHRAHGRSHWEEDSVVSSPDPGSASESGDRYRTDQYQNSHHEPSKIETLIRATQQMIKEEESRIQLRKPSPDQLTTINGAGKRNSVCFANFHQPQLTGVVCRGPPLSNSSPCDHVQQRDGKILSPRDHEYENSPNALSRMSSPNSDRVSKSSVTIAKDYLHANLSPSPAMDHSASPNYYSSHRQYFDKHTYTFTGYALEHLYDSESIRNYSLGCNGTHFDVTSHLRMQQDSAQVHKGTSVIISNGS

Target ORF information:

RefSeq Version	XM_004914545.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SIM bHLH transcription factor 1 (sim1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914545.4

ORF Insert Sequence:

ATGAAAGAAA AATCTAAAAA TGCAGCCCGG ACTAGACGGG AGAAGGAGAA CAGCGAATTT 
TATGAACTGG CTAAATTATT ACCCTTGCCC TCTGCTATCA CCTCCCAGCT GGACAAAGCC 
TCCATCATAC GCCTGACTAC CAGCTATTTA AAGATGAGAG TGGTCTTTCC TGAAGGACTT 
GGTGAAGCCT GGGGGCACAC TAGCAGGCCT AGCACGTTGG ATAATGTTGG CCGGGAACTG 
GGATCCCACC TTTTACAGAC CCTAGATGGA TTCATCTTTG TGGTGGCCCC CGATGGGAAA 
ATCATGTACA TCTCAGAAAC AGCTTCTGTC CATCTGGGCT TATCACAGGT AGAGCTGACT 
GGGAACAGCA TTTATGAATA TATCCACCCT GCTGATCATG ATGAGATGAC AGCAGTTCTG 
ACAGCTCACC AACCCTACCA CTCACACTTT GTACAGGAAT ATGAGATCGA ACGCTCCTTC 
TTCCTACGGA TGAAATGTGT TTTGGCAAAG AGGAATGCGG GGTTGACGTG TGGGGGGTAT 
AAGGTGATAC ATTGCAGTGG GTACCTGAAG ATTCGCCAGT ACAGTCTCGA CATGTCGCCA 
TTTGATGGTT GCTATCAAAA TGTGGGCCTG GTGGCTGTTG GCCATTCCCT CCCTCCCAGT 
GCAGTCACAG AGATTAAACT TCACAGCAAT ATGTTCATGT TCAGAGCCAG CTTAGATATG 
AAGCTCATAT TTTTAGATTC CAGGGTAGCT GAACTAACTG GATATGAGCC TCAAGATCTG 
ATTGAAAAGA CTTTATATCA TCATGTCCAT GGCTGTGACA CATTTCATCT TCGCTGTGCA 
CATCACTTAT TGCTTGTAAA AGGACAGGTC ACCACCAAGT ACTACAGGTT CCTGGCTAAG 
CATGGTGGCT GGGTGTGGGT ACAGAGTTAT GCCACCATTG TACACAACAG CAGGTCCTCC 
AGACCCCACT GTATTGTCAG TGTGAATTAT GTTCTAACGG ATACAGAATA CAAAGGACTA 
CAGCTATCTT TGGATCAAGT TACAACAAAG CCACCATTTT CTTATACAAG CTCAAATCCA 
ACCATAAATG ACAGCAGAAA AAGCAGTAAA GCTCGACTCT CCAACACAAA ATCTAAATCC 
AGAACATCAC CCTACCCACA GTATTCTGGA TTCCATACTG AACGATCTGA ATCAGACCAT 
GAAAGTCAAT GGGGCGGTAG CCCTCTTACT GACACAGCTT CACCACAACT TCTTGATCCC 
ATAGAAAGAC CAAGCTCCCA CCAACACGAT GTATCTTGTG CATATAGACA GTATCCAGAC 
CGAAGTGGCC TTTGCTTTGG ATTTTCCATT GACCATTCAA GGTTAAGTGA TGATAGACAC 
CACCATAGTC AGTCGTGTGA AGGAGGCAGA TGTGAGGCTG GCAGATACTT TCTTGGAAGT 
CCTCAGACTG GAAGAGAGAC CTGGTGGGGT TCTCGATCTG TGTTGCCTGT TCCAAAGACC 
TCTCCAGATG GAAAAGACAT ATATGAGGCT ACCATGCCAC ACATTGCATC AGGTCACAGA 
GCACATGGAA GAAGTCATTG GGAAGAAGAT AGTGTTGTCA GTTCTCCTGA TCCAGGCTCT 
GCAAGTGAAT CAGGGGATCG TTACCGCACT GATCAATATC AAAATAGTCA TCATGAACCA 
AGCAAAATTG AAACTCTTAT ACGTGCTACC CAGCAGATGA TTAAAGAGGA AGAAAGCAGA 
ATACAGCTGC GCAAACCTAG TCCAGATCAG CTAACTACCA TTAATGGAGC TGGTAAAAGA 
AACTCTGTTT GTTTTGCAAA TTTTCACCAG CCACAGTTAA CTGGAGTTGT GTGCCGTGGT 
CCACCTCTAT CTAACTCGTC TCCTTGTGAC CATGTTCAGC AACGGGATGG AAAAATCCTT 
AGTCCTCGTG ACCACGAATA TGAAAACAGC CCAAATGCTT TGTCAAGAAT GAGTAGTCCA 
AATTCTGACA GAGTTTCAAA ATCAAGTGTA ACCATAGCAA AAGATTATTT ACATGCAAAT 
TTGTCCCCAT CACCAGCAAT GGACCATTCT GCATCCCCAA ATTACTACAG TTCTCACAGG 
CAATATTTTG ACAAACATAC TTACACTTTT ACTGGGTATG CTCTTGAACA TCTTTATGAC 
AGTGAATCTA TTAGGAACTA TTCATTGGGA TGTAACGGAA CACATTTTGA TGTGACTTCT 
CATTTAAGGA TGCAACAGGA TTCTGCTCAA GTGCACAAGG GTACATCAGT AATTATTAGT 
AACGGGAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sim1 ( XM_002932141.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932187.2 Xenopus tropicalis SIM bHLH transcription factor 1 (sim1), transcript variant X2, mRNA.

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CloneID	OXa58957
Clone ID Related Accession (Same CDS sequence)	XM_002932141.5
Accession Version	XM_002932141.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	single-minded homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002932141.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGGCA GGGGCAAGCA GAGAGGTGCC GGACTTGGTG AAGCCTGGGG GCACACTAGC 
AGGCCTAGCA CGTTGGATAA TGTTGGCCGG GAACTGGGAT CCCACCTTTT ACAGACCCTA 
GATGGATTCA TCTTTGTGGT GGCCCCCGAT GGGAAAATCA TGTACATCTC AGAAACAGCT 
TCTGTCCATC TGGGCTTATC ACAGGTAGAG CTGACTGGGA ACAGCATTTA TGAATATATC 
CACCCTGCTG ATCATGATGA GATGACAGCA GTTCTGACAG CTCACCAACC CTACCACTCA 
CACTTTGTAC AGGAATATGA GATCGAACGC TCCTTCTTCC TACGGATGAA ATGTGTTTTG 
GCAAAGAGGA ATGCGGGGTT GACGTGTGGG GGGTATAAGG TGATACATTG CAGTGGGTAC 
CTGAAGATTC GCCAGTACAG TCTCGACATG TCGCCATTTG ATGGTTGCTA TCAAAATGTG 
GGCCTGGTGG CTGTTGGCCA TTCCCTCCCT CCCAGTGCAG TCACAGAGAT TAAACTTCAC 
AGCAATATGT TCATGTTCAG AGCCAGCTTA GATATGAAGC TCATATTTTT AGATTCCAGG 
GTAGCTGAAC TAACTGGATA TGAGCCTCAA GATCTGATTG AAAAGACTTT ATATCATCAT 
GTCCATGGCT GTGACACATT TCATCTTCGC TGTGCACATC ACTTATTGCT TGTAAAAGGA 
CAGGTCACCA CCAAGTACTA CAGGTTCCTG GCTAAGCATG GTGGCTGGGT GTGGGTACAG 
AGTTATGCCA CCATTGTACA CAACAGCAGG TCCTCCAGAC CCCACTGTAT TGTCAGTGTG 
AATTATGTTC TAACGGATAC AGAATACAAA GGACTACAGC TATCTTTGGA TCAAGTTACA 
ACAAAGCCAC CATTTTCTTA TACAAGCTCA AATCCAACCA TAAATGACAG CAGAAAAAGC 
AGTAAAGCTC GACTCTCCAA CACAAAATCT AAATCCAGAA CATCACCCTA CCCACAGTAT 
TCTGGATTCC ATACTGAACG ATCTGAATCA GACCATGAAA GTCAATGGGG CGGTAGCCCT 
CTTACTGACA CAGCTTCACC ACAACTTCTT GATCCCATAG AAAGACCAAG CTCCCACCAA 
CACGATGTAT CTTGTGCATA TAGACAGTAT CCAGACCGAA GTGGCCTTTG CTTTGGATTT 
TCCATTGACC ATTCAAGGTT AAGTGATGAT AGACACCACC ATAGTCAGTC GTGTGAAGGA 
GGCAGATGTG AGGCTGGCAG ATACTTTCTT GGAAGTCCTC AGACTGGAAG AGAGACCTGG 
TGGGGTTCTC GATCTGTGTT GCCTGTTCCA AAGACCTCTC CAGATGGAAA AGACATATAT 
GAGGCTACCA TGCCACACAT TGCATCAGGT CACAGAGCAC ATGGAAGAAG TCATTGGGAA 
GAAGATAGTG TTGTCAGTTC TCCTGATCCA GGCTCTGCAA GTGAATCAGG GGATCGTTAC 
CGCACTGATC AATATCAAAA TAGTCATCAT GAACCAAGCA AAATTGAAAC TCTTATACGT 
GCTACCCAGC AGATGATTAA AGAGGAAGAA AGCAGAATAC AGCTGCGCAA ACCTAGTCCA 
GATCAGCTAA CTACCATTAA TGGAGCTGGT AAAAGAAACT CTGTTTGTTT TGCAAATTTT 
CACCAGCCAC AGTTAACTGG AGTTGTGTGC CGTGGTCCAC CTCTATCTAA CTCGTCTCCT 
TGTGACCATG TTCAGCAACG GGATGGAAAA ATCCTTAGTC CTCGTGACCA CGAATATGAA 
AACAGCCCAA ATGCTTTGTC AAGAATGAGT AGTCCAAATT CTGACAGAGT TTCAAAATCA 
AGTGTAACCA TAGCAAAAGA TTATTTACAT GCAAATTTGT CCCCATCACC AGCAATGGAC 
CATTCTGCAT CCCCAAATTA CTACAGTTCT CACAGGCAAT ATTTTGACAA ACATACTTAC 
ACTTTTACTG GGTATGCTCT TGAACATCTT TATGACAGTG AATCTATTAG GAACTATTCA 
TTGGGATGTA ACGGAACACA TTTTGATGTG ACTTCTCATT TAAGGATGCA ACAGGATTCT 
GCTCAAGTGC ACAAGGGTAC ATCAGTAATT ATTAGTAACG GGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932187.2
CDS	64..2211
Translation	MRGRGKQRGAGLGEAWGHTSRPSTLDNVGRELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVLTAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCAHHLLLVKGQVTTKYYRFLAKHGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQVTTKPPFSYTSSNPTINDSRKSSKARLSNTKSKSRTSPYPQYSGFHTERSESDHESQWGGSPLTDTASPQLLDPIERPSSHQHDVSCAYRQYPDRSGLCFGFSIDHSRLSDDRHHHSQSCEGGRCEAGRYFLGSPQTGRETWWGSRSVLPVPKTSPDGKDIYEATMPHIASGHRAHGRSHWEEDSVVSSPDPGSASESGDRYRTDQYQNSHHEPSKIETLIRATQQMIKEEESRIQLRKPSPDQLTTINGAGKRNSVCFANFHQPQLTGVVCRGPPLSNSSPCDHVQQRDGKILSPRDHEYENSPNALSRMSSPNSDRVSKSSVTIAKDYLHANLSPSPAMDHSASPNYYSSHRQYFDKHTYTFTGYALEHLYDSESIRNYSLGCNGTHFDVTSHLRMQQDSAQVHKGTSVIISNGS

Target ORF information:

RefSeq Version	XM_002932141.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SIM bHLH transcription factor 1 (sim1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932141.5

ORF Insert Sequence:

ATGAGGGGCA GGGGCAAGCA GAGAGGTGCC GGACTTGGTG AAGCCTGGGG GCACACTAGC 
AGGCCTAGCA CGTTGGATAA TGTTGGCCGG GAACTGGGAT CCCACCTTTT ACAGACCCTA 
GATGGATTCA TCTTTGTGGT GGCCCCCGAT GGGAAAATCA TGTACATCTC AGAAACAGCT 
TCTGTCCATC TGGGCTTATC ACAGGTAGAG CTGACTGGGA ACAGCATTTA TGAATATATC 
CACCCTGCTG ATCATGATGA GATGACAGCA GTTCTGACAG CTCACCAACC CTACCACTCA 
CACTTTGTAC AGGAATATGA GATCGAACGC TCCTTCTTCC TACGGATGAA ATGTGTTTTG 
GCAAAGAGGA ATGCGGGGTT GACGTGTGGG GGGTATAAGG TGATACATTG CAGTGGGTAC 
CTGAAGATTC GCCAGTACAG TCTCGACATG TCGCCATTTG ATGGTTGCTA TCAAAATGTG 
GGCCTGGTGG CTGTTGGCCA TTCCCTCCCT CCCAGTGCAG TCACAGAGAT TAAACTTCAC 
AGCAATATGT TCATGTTCAG AGCCAGCTTA GATATGAAGC TCATATTTTT AGATTCCAGG 
GTAGCTGAAC TAACTGGATA TGAGCCTCAA GATCTGATTG AAAAGACTTT ATATCATCAT 
GTCCATGGCT GTGACACATT TCATCTTCGC TGTGCACATC ACTTATTGCT TGTAAAAGGA 
CAGGTCACCA CCAAGTACTA CAGGTTCCTG GCTAAGCATG GTGGCTGGGT GTGGGTACAG 
AGTTATGCCA CCATTGTACA CAACAGCAGG TCCTCCAGAC CCCACTGTAT TGTCAGTGTG 
AATTATGTTC TAACGGATAC AGAATACAAA GGACTACAGC TATCTTTGGA TCAAGTTACA 
ACAAAGCCAC CATTTTCTTA TACAAGCTCA AATCCAACCA TAAATGACAG CAGAAAAAGC 
AGTAAAGCTC GACTCTCCAA CACAAAATCT AAATCCAGAA CATCACCCTA CCCACAGTAT 
TCTGGATTCC ATACTGAACG ATCTGAATCA GACCATGAAA GTCAATGGGG CGGTAGCCCT 
CTTACTGACA CAGCTTCACC ACAACTTCTT GATCCCATAG AAAGACCAAG CTCCCACCAA 
CACGATGTAT CTTGTGCATA TAGACAGTAT CCAGACCGAA GTGGCCTTTG CTTTGGATTT 
TCCATTGACC ATTCAAGGTT AAGTGATGAT AGACACCACC ATAGTCAGTC GTGTGAAGGA 
GGCAGATGTG AGGCTGGCAG ATACTTTCTT GGAAGTCCTC AGACTGGAAG AGAGACCTGG 
TGGGGTTCTC GATCTGTGTT GCCTGTTCCA AAGACCTCTC CAGATGGAAA AGACATATAT 
GAGGCTACCA TGCCACACAT TGCATCAGGT CACAGAGCAC ATGGAAGAAG TCATTGGGAA 
GAAGATAGTG TTGTCAGTTC TCCTGATCCA GGCTCTGCAA GTGAATCAGG GGATCGTTAC 
CGCACTGATC AATATCAAAA TAGTCATCAT GAACCAAGCA AAATTGAAAC TCTTATACGT 
GCTACCCAGC AGATGATTAA AGAGGAAGAA AGCAGAATAC AGCTGCGCAA ACCTAGTCCA 
GATCAGCTAA CTACCATTAA TGGAGCTGGT AAAAGAAACT CTGTTTGTTT TGCAAATTTT 
CACCAGCCAC AGTTAACTGG AGTTGTGTGC CGTGGTCCAC CTCTATCTAA CTCGTCTCCT 
TGTGACCATG TTCAGCAACG GGATGGAAAA ATCCTTAGTC CTCGTGACCA CGAATATGAA 
AACAGCCCAA ATGCTTTGTC AAGAATGAGT AGTCCAAATT CTGACAGAGT TTCAAAATCA 
AGTGTAACCA TAGCAAAAGA TTATTTACAT GCAAATTTGT CCCCATCACC AGCAATGGAC 
CATTCTGCAT CCCCAAATTA CTACAGTTCT CACAGGCAAT ATTTTGACAA ACATACTTAC 
ACTTTTACTG GGTATGCTCT TGAACATCTT TATGACAGTG AATCTATTAG GAACTATTCA 
TTGGGATGTA ACGGAACACA TTTTGATGTG ACTTCTCATT TAAGGATGCA ACAGGATTCT 
GCTCAAGTGC ACAAGGGTAC ATCAGTAATT ATTAGTAACG GGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sim1 ( XM_004914546.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914603.1 Xenopus tropicalis SIM bHLH transcription factor 1 (sim1), transcript variant X3, mRNA.

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CloneID	OXa58958
Clone ID Related Accession (Same CDS sequence)	XM_004914546.4
Accession Version	XM_004914546.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	single-minded homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914546.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACATCT CAGAAACAGC TTCTGTCCAT CTGGGCTTAT CACAGGTAGA GCTGACTGGG 
AACAGCATTT ATGAATATAT CCACCCTGCT GATCATGATG AGATGACAGC AGTTCTGACA 
GCTCACCAAC CCTACCACTC ACACTTTGTA CAGGAATATG AGATCGAACG CTCCTTCTTC 
CTACGGATGA AATGTGTTTT GGCAAAGAGG AATGCGGGGT TGACGTGTGG GGGGTATAAG 
GTGATACATT GCAGTGGGTA CCTGAAGATT CGCCAGTACA GTCTCGACAT GTCGCCATTT 
GATGGTTGCT ATCAAAATGT GGGCCTGGTG GCTGTTGGCC ATTCCCTCCC TCCCAGTGCA 
GTCACAGAGA TTAAACTTCA CAGCAATATG TTCATGTTCA GAGCCAGCTT AGATATGAAG 
CTCATATTTT TAGATTCCAG GGTAGCTGAA CTAACTGGAT ATGAGCCTCA AGATCTGATT 
GAAAAGACTT TATATCATCA TGTCCATGGC TGTGACACAT TTCATCTTCG CTGTGCACAT 
CACTTATTGC TTGTAAAAGG ACAGGTCACC ACCAAGTACT ACAGGTTCCT GGCTAAGCAT 
GGTGGCTGGG TGTGGGTACA GAGTTATGCC ACCATTGTAC ACAACAGCAG GTCCTCCAGA 
CCCCACTGTA TTGTCAGTGT GAATTATGTT CTAACGGATA CAGAATACAA AGGACTACAG 
CTATCTTTGG ATCAAGTTAC AACAAAGCCA CCATTTTCTT ATACAAGCTC AAATCCAACC 
ATAAATGACA GCAGAAAAAG CAGTAAAGCT CGACTCTCCA ACACAAAATC TAAATCCAGA 
ACATCACCCT ACCCACAGTA TTCTGGATTC CATACTGAAC GATCTGAATC AGACCATGAA 
AGTCAATGGG GCGGTAGCCC TCTTACTGAC ACAGCTTCAC CACAACTTCT TGATCCCATA 
GAAAGACCAA GCTCCCACCA ACACGATGTA TCTTGTGCAT ATAGACAGTA TCCAGACCGA 
AGTGGCCTTT GCTTTGGATT TTCCATTGAC CATTCAAGGT TAAGTGATGA TAGACACCAC 
CATAGTCAGT CGTGTGAAGG AGGCAGATGT GAGGCTGGCA GATACTTTCT TGGAAGTCCT 
CAGACTGGAA GAGAGACCTG GTGGGGTTCT CGATCTGTGT TGCCTGTTCC AAAGACCTCT 
CCAGATGGAA AAGACATATA TGAGGCTACC ATGCCACACA TTGCATCAGG TCACAGAGCA 
CATGGAAGAA GTCATTGGGA AGAAGATAGT GTTGTCAGTT CTCCTGATCC AGGCTCTGCA 
AGTGAATCAG GGGATCGTTA CCGCACTGAT CAATATCAAA ATAGTCATCA TGAACCAAGC 
AAAATTGAAA CTCTTATACG TGCTACCCAG CAGATGATTA AAGAGGAAGA AAGCAGAATA 
CAGCTGCGCA AACCTAGTCC AGATCAGCTA ACTACCATTA ATGGAGCTGG TAAAAGAAAC 
TCTGTTTGTT TTGCAAATTT TCACCAGCCA CAGTTAACTG GAGTTGTGTG CCGTGGTCCA 
CCTCTATCTA ACTCGTCTCC TTGTGACCAT GTTCAGCAAC GGGATGGAAA AATCCTTAGT 
CCTCGTGACC ACGAATATGA AAACAGCCCA AATGCTTTGT CAAGAATGAG TAGTCCAAAT 
TCTGACAGAG TTTCAAAATC AAGTGTAACC ATAGCAAAAG ATTATTTACA TGCAAATTTG 
TCCCCATCAC CAGCAATGGA CCATTCTGCA TCCCCAAATT ACTACAGTTC TCACAGGCAA 
TATTTTGACA AACATACTTA CACTTTTACT GGGTATGCTC TTGAACATCT TTATGACAGT 
GAATCTATTA GGAACTATTC ATTGGGATGT AACGGAACAC ATTTTGATGT GACTTCTCAT 
TTAAGGATGC AACAGGATTC TGCTCAAGTG CACAAGGGTA CATCAGTAAT TATTAGTAAC 
GGGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914603.1
CDS	346..2334
Translation	MYISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVLTAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCAHHLLLVKGQVTTKYYRFLAKHGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQVTTKPPFSYTSSNPTINDSRKSSKARLSNTKSKSRTSPYPQYSGFHTERSESDHESQWGGSPLTDTASPQLLDPIERPSSHQHDVSCAYRQYPDRSGLCFGFSIDHSRLSDDRHHHSQSCEGGRCEAGRYFLGSPQTGRETWWGSRSVLPVPKTSPDGKDIYEATMPHIASGHRAHGRSHWEEDSVVSSPDPGSASESGDRYRTDQYQNSHHEPSKIETLIRATQQMIKEEESRIQLRKPSPDQLTTINGAGKRNSVCFANFHQPQLTGVVCRGPPLSNSSPCDHVQQRDGKILSPRDHEYENSPNALSRMSSPNSDRVSKSSVTIAKDYLHANLSPSPAMDHSASPNYYSSHRQYFDKHTYTFTGYALEHLYDSESIRNYSLGCNGTHFDVTSHLRMQQDSAQVHKGTSVIISNGS

Target ORF information:

RefSeq Version	XM_004914546.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SIM bHLH transcription factor 1 (sim1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914546.4

ORF Insert Sequence:

ATGTACATCT CAGAAACAGC TTCTGTCCAT CTGGGCTTAT CACAGGTAGA GCTGACTGGG 
AACAGCATTT ATGAATATAT CCACCCTGCT GATCATGATG AGATGACAGC AGTTCTGACA 
GCTCACCAAC CCTACCACTC ACACTTTGTA CAGGAATATG AGATCGAACG CTCCTTCTTC 
CTACGGATGA AATGTGTTTT GGCAAAGAGG AATGCGGGGT TGACGTGTGG GGGGTATAAG 
GTGATACATT GCAGTGGGTA CCTGAAGATT CGCCAGTACA GTCTCGACAT GTCGCCATTT 
GATGGTTGCT ATCAAAATGT GGGCCTGGTG GCTGTTGGCC ATTCCCTCCC TCCCAGTGCA 
GTCACAGAGA TTAAACTTCA CAGCAATATG TTCATGTTCA GAGCCAGCTT AGATATGAAG 
CTCATATTTT TAGATTCCAG GGTAGCTGAA CTAACTGGAT ATGAGCCTCA AGATCTGATT 
GAAAAGACTT TATATCATCA TGTCCATGGC TGTGACACAT TTCATCTTCG CTGTGCACAT 
CACTTATTGC TTGTAAAAGG ACAGGTCACC ACCAAGTACT ACAGGTTCCT GGCTAAGCAT 
GGTGGCTGGG TGTGGGTACA GAGTTATGCC ACCATTGTAC ACAACAGCAG GTCCTCCAGA 
CCCCACTGTA TTGTCAGTGT GAATTATGTT CTAACGGATA CAGAATACAA AGGACTACAG 
CTATCTTTGG ATCAAGTTAC AACAAAGCCA CCATTTTCTT ATACAAGCTC AAATCCAACC 
ATAAATGACA GCAGAAAAAG CAGTAAAGCT CGACTCTCCA ACACAAAATC TAAATCCAGA 
ACATCACCCT ACCCACAGTA TTCTGGATTC CATACTGAAC GATCTGAATC AGACCATGAA 
AGTCAATGGG GCGGTAGCCC TCTTACTGAC ACAGCTTCAC CACAACTTCT TGATCCCATA 
GAAAGACCAA GCTCCCACCA ACACGATGTA TCTTGTGCAT ATAGACAGTA TCCAGACCGA 
AGTGGCCTTT GCTTTGGATT TTCCATTGAC CATTCAAGGT TAAGTGATGA TAGACACCAC 
CATAGTCAGT CGTGTGAAGG AGGCAGATGT GAGGCTGGCA GATACTTTCT TGGAAGTCCT 
CAGACTGGAA GAGAGACCTG GTGGGGTTCT CGATCTGTGT TGCCTGTTCC AAAGACCTCT 
CCAGATGGAA AAGACATATA TGAGGCTACC ATGCCACACA TTGCATCAGG TCACAGAGCA 
CATGGAAGAA GTCATTGGGA AGAAGATAGT GTTGTCAGTT CTCCTGATCC AGGCTCTGCA 
AGTGAATCAG GGGATCGTTA CCGCACTGAT CAATATCAAA ATAGTCATCA TGAACCAAGC 
AAAATTGAAA CTCTTATACG TGCTACCCAG CAGATGATTA AAGAGGAAGA AAGCAGAATA 
CAGCTGCGCA AACCTAGTCC AGATCAGCTA ACTACCATTA ATGGAGCTGG TAAAAGAAAC 
TCTGTTTGTT TTGCAAATTT TCACCAGCCA CAGTTAACTG GAGTTGTGTG CCGTGGTCCA 
CCTCTATCTA ACTCGTCTCC TTGTGACCAT GTTCAGCAAC GGGATGGAAA AATCCTTAGT 
CCTCGTGACC ACGAATATGA AAACAGCCCA AATGCTTTGT CAAGAATGAG TAGTCCAAAT 
TCTGACAGAG TTTCAAAATC AAGTGTAACC ATAGCAAAAG ATTATTTACA TGCAAATTTG 
TCCCCATCAC CAGCAATGGA CCATTCTGCA TCCCCAAATT ACTACAGTTC TCACAGGCAA 
TATTTTGACA AACATACTTA CACTTTTACT GGGTATGCTC TTGAACATCT TTATGACAGT 
GAATCTATTA GGAACTATTC ATTGGGATGT AACGGAACAC ATTTTGATGT GACTTCTCAT 
TTAAGGATGC AACAGGATTC TGCTCAAGTG CACAAGGGTA CATCAGTAAT TATTAGTAAC 
GGGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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sim1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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