SIM1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SIM1
Entrez Gene ID	6492
Full Name	single-minded family bHLH transcription factor 1
Synonyms	bHLHe14
General protein information	Preferred Names single-minded family bHLH transcription factor 1 Names single-minded homolog 1 class E basic helix-loop-helix protein 14
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q16.3
Summary	SIM1 and SIM2 genes are Drosophila single-minded (sim) gene homologs. SIM1 transcript was detected only in fetal kidney out of various adult and fetal tissues tested. Since the sim gene plays an important role in Drosophila development and has peak levels of expression during the period of neurogenesis,it was proposed that the human SIM gene is a candidate for involvement in certain dysmorphic features (particularly the facial and skull characteristics), abnormalities of brain development, and/or cognitive disability of Down syndrome. [provided by RefSeq, Jul 2008].
Disorder MIM:	603128
Disorder Html:	Obesity, severe, 601665 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001374769.1	NP_001361698.1	single-minded homolog 1
XM_017011197.1	XP_016866686.1	single-minded homolog 1 isoform X1
XM_011536072.2	XP_011534374.1	single-minded homolog 1 isoform X2
NM_005068.2	NP_005059.2	single-minded homolog 1
XM_005267100.2	XP_005267157.1	single-minded homolog 1 isoform X1
NM_005068.3	NP_005059.2	single-minded homolog 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SIM1	NP_001074957.1
Danio rerio (zebrafish)	sim1a	NP_835740.1
Xenopus tropicalis (tropical clawed frog)	sim1	XP_004914602.1
Homo sapiens (human)	SIM1	NP_005059.2
Canis lupus familiaris (dog)	SIM1	XP_539058.2
Mus musculus (house mouse)	Sim1	NP_035506.2
Gallus gallus (chicken)	SIM1	XP_004940413.1
	SIM1	XP_001086054.1
Bos taurus (cattle)	SIM1	NP_001179901.1
Rattus norvegicus (Norway rat)	Sim1	NP_001101111.1

Gene Ontology
Bibliography

Related articles in PubMed

Aberrant single-minded homolog 1 methylation as a potential biomarker for cervical cancer.
Kim HJ, Kim CY, Jin J, Bae MK, Kim YH, Ju W, Kim YH, Kim SC
Diagnostic cytopathology46(1)15-21(2018 Jan)

Copy number variations in "classical" obesity candidate genes are not frequently associated with severe early-onset obesity in children.
Windholz J, Kovacs P, Schlicke M, Franke C, Mahajan A, Morris AP, Lemke JR, Klammt J, Kiess W, Sch?neberg T, Pf?ffle R, K?rner A
Journal of pediatric endocrinology & metabolism : JPEM30(5)507-515(2017 May)

Genetic analysis of single-minded 1 gene in early-onset severely obese children and adolescents.
Stanikova D, Buzga M, Krumpolec P, Skopkova M, Surova M, Ukropcova B, Ticha L, Petrasova M, Gabcova D, Huckova M, Piskorova L, Bozensky J, Mokan M, Ukropec J, Zavacka I, Klimes I, Stanik J, Gasperikova D
PloS one12(5)e0177222(2017)

Copy number variation (CNV) analysis and mutation analysis of the 6q14.1-6q16.3 genes SIM1 and MRAP2 in Prader Willi like patients.
Geets E, Zegers D, Beckers S, Verrijken A, Massa G, Van Hoorenbeeck K, Verhulst S, Van Gaal L, Van Hul W
Molecular genetics and metabolism117(3)383-8(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

identified a novel SIM1 variant, p.D134N, in 4 obese individuals from a single pedigree which is also associated with lower preference for certain foods
Title: Genetic analysis of single-minded 1 gene in early-onset severely obese children and adolescents.

no gene harboring deletions were identified in the SIM1 and MRAP2 regions in the Prader Willi like (PWL) cohort; further functional analysis of p.P352S found in SIM1 and p.A40S found in MRAP2 is useful; this would provide further support for possible role of SIM1 and MRAP2 in the pathogenesis of the PWL phenotype in a limited number of patients
Title: Copy number variation (CNV) analysis and mutation analysis of the 6q14.1-6q16.3 genes SIM1 and MRAP2 in Prader Willi like patients.

Genotype-phenotype correlations confirmed the major role for SIM1 haploinsufficiency in obesity and the Prader-Willi-like phenotype
Title: Incomplete penetrance and phenotypic variability of 6q16 deletions including SIM1.

Aberrant DNA methylation of the DLX4 and SIM1 genes may be a novel progression marker for uterine cervical low-grade squamous intraepithelial lesions.
Title: Aberrant DNA methylation of DLX4 and SIM1 is a predictive marker for disease progression of uterine cervical low-grade squamous intraepithelial lesion.

Severe loss-of-function SIM1 mutations can be associated with a spectrum of developmental delay phenotypes and obesity.
Title: Identification of two novel loss-of-function SIM1 mutations in two overweight children with developmental delay.

The following SIM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu60520	XM_011536072.2 Latest version!	Homo sapiens SIM bHLH transcription factor 1 (SIM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu18964	XM_017011197.1 Latest version!	Homo sapiens SIM bHLH transcription factor 1 (SIM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu18964	XM_005267100.2 Latest version!	Homo sapiens SIM bHLH transcription factor 1 (SIM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu18964	NM_001374769.1 Latest version!	Homo sapiens SIM bHLH transcription factor 1 (SIM1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu18964	NM_005068.3 Latest version!	Homo sapiens SIM bHLH transcription factor 1 (SIM1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu18964	NM_005068.2	Homo sapiens SIM bHLH transcription factor 1 (SIM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SIM1 ( NM_005068.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005059.2 Homo sapiens SIM bHLH transcription factor 1 (SIM1), mRNA.

Price & Availability↑

CloneID	OHu18964
Clone ID Related Accession (Same CDS sequence)	NM_001374769.1 , XM_017011197.1 , NM_005068.2 , XM_005267100.2 , NM_005068.3
Accession Version	NM_005068.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Homo sapiens(human)
Product	single-minded homolog 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U70212.1. This sequence is a reference standard in the RefSeqGene project. On Jun 26, 2002 this sequence version replaced NM_005068.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U70212.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END##

ATGAAAGAAA AGTCCAAAAA TGCTGCGCGG ACTAGGAGGG AGAAGGAAAA CAGTGAATTT 
TATGAACTGG CTAAATTACT GCCTTTGCCC TCGGCTATCA CCTCGCAGCT GGACAAAGCA 
TCCATAATCA GACTCACGAC CAGCTATCTC AAAATGAGAG TGGTGTTCCC AGAAGGGCTC 
GGCGAGGCGT GGGGCCACTC AAGTCGGACC AGCCCCCTGG ACAACGTTGG CCGAGAACTG 
GGCTCCCATC TGCTCCAGAC CCTGGATGGC TTCATCTTCG TGGTAGCCCC AGATGGGAAG 
ATCATGTACA TCTCAGAGAC AGCCTCAGTC CACTTGGGTC TTTCTCAGGT AGAGCTGACC 
GGAAACAGCA TTTATGAATA CATTCACCCG GCAGACCACG ACGAGATGAC GGCGGTGCTC 
ACCGCCCATC AACCCTACCA CTCTCACTTC GTGCAGGAGT ATGAGATCGA GCGCTCCTTC 
TTCCTGAGGA TGAAGTGCGT CTTGGCCAAG CGTAACGCCG GCCTCACCTG TGGCGGCTAC 
AAGGTCATCC ACTGCAGCGG CTACTTGAAG ATCCGCCAGT ACAGCCTGGA CATGTCCCCC 
TTCGACGGCT GCTACCAAAA CGTGGGCCTG GTGGCCGTGG GCCACTCGCT GCCTCCCAGC 
GCCGTCACGG AGATCAAGCT ACACAGCAAT ATGTTTATGT TCCGCGCCAG CCTGGACATG 
AAGCTCATCT TTCTGGACTC CAGGGTGGCG GAGCTGACGG GGTACGAACC TCAGGACCTG 
ATTGAGAAGA CTCTGTACCA CCATGTGCAC GGCTGCGACA CCTTCCACCT GCGCTGCGCG 
CACCATTTGC TGCTGGTGAA GGGACAGGTG ACCACCAAGT ACTACAGGTT CCTGGCGAAA 
CACGGCGGCT GGGTATGGGT GCAGAGCTAC GCGACCATCG TGCACAACAG TCGCTCCTCC 
AGGCCACACT GTATCGTCAG CGTCAACTAT GTCCTCACAG ACACAGAATA CAAAGGGCTG 
CAGCTCTCCC TGGATCAGAT CTCAGCCTCC AAACCAGCCT TCTCCTATAC CAGCAGCTCC 
ACCCCCACCA TGACTGACAA CAGAAAGGGG GCCAAATCCC GGCTCTCCAG CTCAAAGTCA 
AAATCCAGGA CTTCCCCATA CCCTCAGTAT TCGGGATTTC ACACAGAAAG ATCGGAATCT 
GATCATGACA GCCAGTGGGG CGGAAGTCCC TTGACCGACA CGGCCTCTCC GCAGCTTCTG 
GACCCCGCCG ATAGGCCTGG CTCCCAGCAC GACGCATCGT GCGCCTACAG ACAGTTTTCG 
GACCGCAGCT CTCTCTGCTA TGGCTTTGCG CTTGACCACT CGAGGCTGGT GGAAGAGAGG 
CATTTCCATA CCCAGGCCTG TGAAGGAGGC CGATGTGAGG CAGGCAGGTA CTTCCTGGGA 
ACGCCGCAGG CCGGGAGGGA GCCCTGGTGG GGCTCTCGCG CAGCCTTGCC CCTGACAAAG 
GCCTCCCCAG AAAGCAGAGA AGCCTATGAA AACAGCATGC CTCACATCGC TTCAGTCCAC 
AGGATCCATG GGCGAGGTCA TTGGGATGAA GATAGTGTGG TCAGTTCTCC AGACCCTGGG 
TCGGCCAGTG AATCAGGTGA CCGATATCGT ACTGAGCAGT ATCAAAGTAG CCCACATGAA 
CCCAGCAAAA TTGAAACTCT TATAAGAGCC ACTCAGCAAA TGATTAAAGA AGAAGAGAAC 
AGATTACAGC TAAGGAAAGC CCCCTCAGAC CAACTGGCTT CCATTAATGG GGCTGGGAAA 
AAACACTCCC TGTGTTTTGC AAACTACCAA CAGCCCCCAC CAACAGGTGA AGTCTGCCAT 
GGCTCTGCTC TTGCCAACAC TTCACCATGT GACCATATCC AGCAGAGAGA GGGAAAAATG 
TTGAGCCCCC ATGAAAATGA CTATGACAAC AGTCCCACCG CACTATCTCG GATAAGTAGT 
CCCAATTCGG ATCGCATTTC AAAATCCAGT TTGATCCTAG CTAAAGACTA TCTGCATTCG 
GATATATCTC CTCATCAGAC AGCAGGAGAC CACCCTACTG TCTCTCCAAA CTGCTTTGGC 
TCTCACCGGC AGTATTTTGA CAAGCATGCT TACACATTAA CTGGATATGC CCTGGAGCAC 
TTATATGACA GCGAAACCAT TAGAAACTAT TCCTTGGGCT GTAATGGCTC ACACTTTGAT 
GTAACTTCCC ATCTGAGGAT GCAACCAGAC CCAGCACAAG GACACAAGGG AACATCTGTT 
ATAATAACCA ACGGAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005059.2
CDS	208..2508
Translation	MKEKSKNAARTRREKENSEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRVVFPEGLGEAWGHSSRTSPLDNVGRELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVLTAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCAHHLLLVKGQVTTKYYRFLAKHGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQISASKPAFSYTSSSTPTMTDNRKGAKSRLSSSKSKSRTSPYPQYSGFHTERSESDHDSQWGGSPLTDTASPQLLDPADRPGSQHDASCAYRQFSDRSSLCYGFALDHSRLVEERHFHTQACEGGRCEAGRYFLGTPQAGREPWWGSRAALPLTKASPESREAYENSMPHIASVHRIHGRGHWDEDSVVSSPDPGSASESGDRYRTEQYQSSPHEPSKIETLIRATQQMIKEEENRLQLRKAPSDQLASINGAGKKHSLCFANYQQPPPTGEVCHGSALANTSPCDHIQQREGKMLSPHENDYDNSPTALSRISSPNSDRISKSSLILAKDYLHSDISPHQTAGDHPTVSPNCFGSHRQYFDKHAYTLTGYALEHLYDSETIRNYSLGCNGSHFDVTSHLRMQPDPAQGHKGTSVIITNGS

Target ORF information:

RefSeq Version	NM_005068.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SIM bHLH transcription factor 1 (SIM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005068.2

ORF Insert Sequence:

ATGAAAGAAA AGTCCAAAAA TGCTGCGCGG ACTAGGAGGG AGAAGGAAAA CAGTGAATTT 
TATGAACTGG CTAAATTACT GCCTTTGCCC TCGGCTATCA CCTCGCAGCT GGACAAAGCA 
TCCATAATCA GACTCACGAC CAGCTATCTC AAAATGAGAG TGGTGTTCCC AGAAGGGCTC 
GGCGAGGCGT GGGGCCACTC AAGTCGGACC AGCCCCCTGG ACAACGTTGG CCGAGAACTG 
GGCTCCCATC TGCTCCAGAC CCTGGATGGC TTCATCTTCG TGGTAGCCCC AGATGGGAAG 
ATCATGTACA TCTCAGAGAC AGCCTCAGTC CACTTGGGTC TTTCTCAGGT AGAGCTGACC 
GGAAACAGCA TTTATGAATA CATTCACCCG GCAGACCACG ACGAGATGAC GGCGGTGCTC 
ACCGCCCATC AACCCTACCA CTCTCACTTC GTGCAGGAGT ATGAGATCGA GCGCTCCTTC 
TTCCTGAGGA TGAAGTGCGT CTTGGCCAAG CGTAACGCCG GCCTCACCTG TGGCGGCTAC 
AAGGTCATCC ACTGCAGCGG CTACTTGAAG ATCCGCCAGT ACAGCCTGGA CATGTCCCCC 
TTCGACGGCT GCTACCAAAA CGTGGGCCTG GTGGCCGTGG GCCACTCGCT GCCTCCCAGC 
GCCGTCACGG AGATCAAGCT ACACAGCAAT ATGTTTATGT TCCGCGCCAG CCTGGACATG 
AAGCTCATCT TTCTGGACTC CAGGGTGGCG GAGCTGACGG GGTACGAACC TCAGGACCTG 
ATTGAGAAGA CTCTGTACCA CCATGTGCAC GGCTGCGACA CCTTCCACCT GCGCTGCGCG 
CACCATTTGC TGCTGGTGAA GGGACAGGTG ACCACCAAGT ACTACAGGTT CCTGGCGAAA 
CACGGCGGCT GGGTATGGGT GCAGAGCTAC GCGACCATCG TGCACAACAG TCGCTCCTCC 
AGGCCACACT GTATCGTCAG CGTCAACTAT GTCCTCACAG ACACAGAATA CAAAGGGCTG 
CAGCTCTCCC TGGATCAGAT CTCAGCCTCC AAACCAGCCT TCTCCTATAC CAGCAGCTCC 
ACCCCCACCA TGACTGACAA CAGAAAGGGG GCCAAATCCC GGCTCTCCAG CTCAAAGTCA 
AAATCCAGGA CTTCCCCATA CCCTCAGTAT TCGGGATTTC ACACAGAAAG ATCGGAATCT 
GATCATGACA GCCAGTGGGG CGGAAGTCCC TTGACCGACA CGGCCTCTCC GCAGCTTCTG 
GACCCCGCCG ATAGGCCTGG CTCCCAGCAC GACGCATCGT GCGCCTACAG ACAGTTTTCG 
GACCGCAGCT CTCTCTGCTA TGGCTTTGCG CTTGACCACT CGAGGCTGGT GGAAGAGAGG 
CATTTCCATA CCCAGGCCTG TGAAGGAGGC CGATGTGAGG CAGGCAGGTA CTTCCTGGGA 
ACGCCGCAGG CCGGGAGGGA GCCCTGGTGG GGCTCTCGCG CAGCCTTGCC CCTGACAAAG 
GCCTCCCCAG AAAGCAGAGA AGCCTATGAA AACAGCATGC CTCACATCGC TTCAGTCCAC 
AGGATCCATG GGCGAGGTCA TTGGGATGAA GATAGTGTGG TCAGTTCTCC AGACCCTGGG 
TCGGCCAGTG AATCAGGTGA CCGATATCGT ACTGAGCAGT ATCAAAGTAG CCCACATGAA 
CCCAGCAAAA TTGAAACTCT TATAAGAGCC ACTCAGCAAA TGATTAAAGA AGAAGAGAAC 
AGATTACAGC TAAGGAAAGC CCCCTCAGAC CAACTGGCTT CCATTAATGG GGCTGGGAAA 
AAACACTCCC TGTGTTTTGC AAACTACCAA CAGCCCCCAC CAACAGGTGA AGTCTGCCAT 
GGCTCTGCTC TTGCCAACAC TTCACCATGT GACCATATCC AGCAGAGAGA GGGAAAAATG 
TTGAGCCCCC ATGAAAATGA CTATGACAAC AGTCCCACCG CACTATCTCG GATAAGTAGT 
CCCAATTCGG ATCGCATTTC AAAATCCAGT TTGATCCTAG CTAAAGACTA TCTGCATTCG 
GATATATCTC CTCATCAGAC AGCAGGAGAC CACCCTACTG TCTCTCCAAA CTGCTTTGGC 
TCTCACCGGC AGTATTTTGA CAAGCATGCT TACACATTAA CTGGATATGC CCTGGAGCAC 
TTATATGACA GCGAAACCAT TAGAAACTAT TCCTTGGGCT GTAATGGCTC ACACTTTGAT 
GTAACTTCCC ATCTGAGGAT GCAACCAGAC CCAGCACAAG GACACAAGGG AACATCTGTT 
ATAATAACCA ACGGAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIM1 ( XM_011536072.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534374.1 Homo sapiens SIM bHLH transcription factor 1 (SIM1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu60520
Clone ID Related Accession (Same CDS sequence)	XM_011536072.2
Accession Version	XM_011536072.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	single-minded homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011536072.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAA AGTCCAAAAA TGCTGCGCGG ACTAGGAGGG AGAAGGAAAA CAGTGAATTT 
TATGAACTGG CTAAATTACT GCCTTTGCCC TCGGCTATCA CCTCGCAGCT GGACAAAGCA 
TCCATAATCA GACTCACGAC CAGCTATCTC AAAATGAGAG TGGTGTTCCC AGAAGGGCTC 
GGCGAGGCGT GGGGCCACTC AAGTCGGACC AGCCCCCTGG ACAACGTTGG CCGAGAACTG 
GGCTCCCATC TGCTCCAGAC CCTGGATGGC TTCATCTTCG TGGTAGCCCC AGATGGGAAG 
ATCATGTACA TCTCAGAGAC AGCCTCAGTC CACTTGGGTC TTTCTCAGGT AGAGCTGACC 
GGAAACAGCA TTTATGAATA CATTCACCCG GCAGACCACG ACGAGATGAC GGCGGTGCTC 
ACCGCCCATC AACCCTACCA CTCTCACTTC GTGCAGGAGT ATGAGATCGA GCGCTCCTTC 
TTCCTGAGGA TGAAGTGCGT CTTGGCCAAG CGTAACGCCG GCCTCACCTG TGGCGGCTAC 
AAGGTCATCC ACTGCAGCGG CTACTTGAAG ATCCGCCAGT ACAGCCTGGA CATGTCCCCC 
TTCGACGGCT GCTACCAAAA CGTGGGCCTG GTGGCCGTGG GCCACTCGCT GCCTCCCAGC 
GCCGTCACGG AGATCAAGCT ACACAGCAAT ATGTTTATGT TCCGCGCCAG CCTGGACATG 
AAGCTCATCT TTCTGGACTC CAGGGTGGCG GAGCTGACGG GGTACGAACC TCAGGACCTG 
ATTGAGAAGA CTCTGTACCA CCATGTGCAC GGCTGCGACA CCTTCCACCT GCGCTGCGCG 
CACCATTTGC TGCTGGTGAA GGGACAGGTG ACCACCAAGT ACTACAGGTT CCTGGCGAAA 
CACGGCGGCT GGGTATGGGT GCAGAGCTAC GCGACCATCG TGCACAACAG TCGCTCCTCC 
AGGCCACACT GTATCGTCAG CGTCAACTAT GTCCTCACAG ACACAGAATA CAAAGGGCTG 
CAGCTCTCCC TGGATCAGAT CTCAGCCTCC AAACCAGCCT TCTCCTATAC CAGCAGCTCC 
ACCCCCACCA TGACTGACAA CAGAAAGGGG GCCAAATCCC GGCTCTCCAG CTCAAAGTCA 
AAATCCAGGA CTTCCCCATA CCCTCAGTAT TCGGGATTTC ACACAGAAAG ATCGGAATCT 
GATCATGACA GCCAGTGGGG CGGAAGTCCC TTGACCGACA CGGCCTCTCC GCAGCTTCTG 
GACCCCGCCG ATAGGCCTGG CTCCCAGCAC GACGCATCGT GCGCCTACAG ACAGTTTTCG 
GACCGCAGCT CTCTCTGCTA TGGCTTTGCG CTTGACCACT CGAGGCTGGT GGAAGAGAGG 
CATTTCCATA CCCAGGCCTG TGAAGGAGGC CGATGTGAGG CAGGCAGGGC GAGGTCATTG 
GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534374.1
CDS	784..2229
Translation	MKEKSKNAARTRREKENSEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRVVFPEGLGEAWGHSSRTSPLDNVGRELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVLTAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCAHHLLLVKGQVTTKYYRFLAKHGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQISASKPAFSYTSSSTPTMTDNRKGAKSRLSSSKSKSRTSPYPQYSGFHTERSESDHDSQWGGSPLTDTASPQLLDPADRPGSQHDASCAYRQFSDRSSLCYGFALDHSRLVEERHFHTQACEGGRCEAGRARSLG

Target ORF information:

RefSeq Version	XM_011536072.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SIM bHLH transcription factor 1 (SIM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536072.2

ORF Insert Sequence:

ATGAAAGAAA AGTCCAAAAA TGCTGCGCGG ACTAGGAGGG AGAAGGAAAA CAGTGAATTT 
TATGAACTGG CTAAATTACT GCCTTTGCCC TCGGCTATCA CCTCGCAGCT GGACAAAGCA 
TCCATAATCA GACTCACGAC CAGCTATCTC AAAATGAGAG TGGTGTTCCC AGAAGGGCTC 
GGCGAGGCGT GGGGCCACTC AAGTCGGACC AGCCCCCTGG ACAACGTTGG CCGAGAACTG 
GGCTCCCATC TGCTCCAGAC CCTGGATGGC TTCATCTTCG TGGTAGCCCC AGATGGGAAG 
ATCATGTACA TCTCAGAGAC AGCCTCAGTC CACTTGGGTC TTTCTCAGGT AGAGCTGACC 
GGAAACAGCA TTTATGAATA CATTCACCCG GCAGACCACG ACGAGATGAC GGCGGTGCTC 
ACCGCCCATC AACCCTACCA CTCTCACTTC GTGCAGGAGT ATGAGATCGA GCGCTCCTTC 
TTCCTGAGGA TGAAGTGCGT CTTGGCCAAG CGTAACGCCG GCCTCACCTG TGGCGGCTAC 
AAGGTCATCC ACTGCAGCGG CTACTTGAAG ATCCGCCAGT ACAGCCTGGA CATGTCCCCC 
TTCGACGGCT GCTACCAAAA CGTGGGCCTG GTGGCCGTGG GCCACTCGCT GCCTCCCAGC 
GCCGTCACGG AGATCAAGCT ACACAGCAAT ATGTTTATGT TCCGCGCCAG CCTGGACATG 
AAGCTCATCT TTCTGGACTC CAGGGTGGCG GAGCTGACGG GGTACGAACC TCAGGACCTG 
ATTGAGAAGA CTCTGTACCA CCATGTGCAC GGCTGCGACA CCTTCCACCT GCGCTGCGCG 
CACCATTTGC TGCTGGTGAA GGGACAGGTG ACCACCAAGT ACTACAGGTT CCTGGCGAAA 
CACGGCGGCT GGGTATGGGT GCAGAGCTAC GCGACCATCG TGCACAACAG TCGCTCCTCC 
AGGCCACACT GTATCGTCAG CGTCAACTAT GTCCTCACAG ACACAGAATA CAAAGGGCTG 
CAGCTCTCCC TGGATCAGAT CTCAGCCTCC AAACCAGCCT TCTCCTATAC CAGCAGCTCC 
ACCCCCACCA TGACTGACAA CAGAAAGGGG GCCAAATCCC GGCTCTCCAG CTCAAAGTCA 
AAATCCAGGA CTTCCCCATA CCCTCAGTAT TCGGGATTTC ACACAGAAAG ATCGGAATCT 
GATCATGACA GCCAGTGGGG CGGAAGTCCC TTGACCGACA CGGCCTCTCC GCAGCTTCTG 
GACCCCGCCG ATAGGCCTGG CTCCCAGCAC GACGCATCGT GCGCCTACAG ACAGTTTTCG 
GACCGCAGCT CTCTCTGCTA TGGCTTTGCG CTTGACCACT CGAGGCTGGT GGAAGAGAGG 
CATTTCCATA CCCAGGCCTG TGAAGGAGGC CGATGTGAGG CAGGCAGGGC GAGGTCATTG 
GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIM1 ( XM_017011197.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866686.1 Homo sapiens SIM bHLH transcription factor 1 (SIM1), transcript variant X2, mRNA.

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CloneID	OHu18964
Clone ID Related Accession (Same CDS sequence)	NM_001374769.1 , XM_017011197.1 , NM_005068.2 , XM_005267100.2 , NM_005068.3
Accession Version	XM_017011197.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	single-minded homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGAAAGAAA AGTCCAAAAA TGCTGCGCGG ACTAGGAGGG AGAAGGAAAA CAGTGAATTT 
TATGAACTGG CTAAATTACT GCCTTTGCCC TCGGCTATCA CCTCGCAGCT GGACAAAGCA 
TCCATAATCA GACTCACGAC CAGCTATCTC AAAATGAGAG TGGTGTTCCC AGAAGGGCTC 
GGCGAGGCGT GGGGCCACTC AAGTCGGACC AGCCCCCTGG ACAACGTTGG CCGAGAACTG 
GGCTCCCATC TGCTCCAGAC CCTGGATGGC TTCATCTTCG TGGTAGCCCC AGATGGGAAG 
ATCATGTACA TCTCAGAGAC AGCCTCAGTC CACTTGGGTC TTTCTCAGGT AGAGCTGACC 
GGAAACAGCA TTTATGAATA CATTCACCCG GCAGACCACG ACGAGATGAC GGCGGTGCTC 
ACCGCCCATC AACCCTACCA CTCTCACTTC GTGCAGGAGT ATGAGATCGA GCGCTCCTTC 
TTCCTGAGGA TGAAGTGCGT CTTGGCCAAG CGTAACGCCG GCCTCACCTG TGGCGGCTAC 
AAGGTCATCC ACTGCAGCGG CTACTTGAAG ATCCGCCAGT ACAGCCTGGA CATGTCCCCC 
TTCGACGGCT GCTACCAAAA CGTGGGCCTG GTGGCCGTGG GCCACTCGCT GCCTCCCAGC 
GCCGTCACGG AGATCAAGCT ACACAGCAAT ATGTTTATGT TCCGCGCCAG CCTGGACATG 
AAGCTCATCT TTCTGGACTC CAGGGTGGCG GAGCTGACGG GGTACGAACC TCAGGACCTG 
ATTGAGAAGA CTCTGTACCA CCATGTGCAC GGCTGCGACA CCTTCCACCT GCGCTGCGCG 
CACCATTTGC TGCTGGTGAA GGGACAGGTG ACCACCAAGT ACTACAGGTT CCTGGCGAAA 
CACGGCGGCT GGGTATGGGT GCAGAGCTAC GCGACCATCG TGCACAACAG TCGCTCCTCC 
AGGCCACACT GTATCGTCAG CGTCAACTAT GTCCTCACAG ACACAGAATA CAAAGGGCTG 
CAGCTCTCCC TGGATCAGAT CTCAGCCTCC AAACCAGCCT TCTCCTATAC CAGCAGCTCC 
ACCCCCACCA TGACTGACAA CAGAAAGGGG GCCAAATCCC GGCTCTCCAG CTCAAAGTCA 
AAATCCAGGA CTTCCCCATA CCCTCAGTAT TCGGGATTTC ACACAGAAAG ATCGGAATCT 
GATCATGACA GCCAGTGGGG CGGAAGTCCC TTGACCGACA CGGCCTCTCC GCAGCTTCTG 
GACCCCGCCG ATAGGCCTGG CTCCCAGCAC GACGCATCGT GCGCCTACAG ACAGTTTTCG 
GACCGCAGCT CTCTCTGCTA TGGCTTTGCG CTTGACCACT CGAGGCTGGT GGAAGAGAGG 
CATTTCCATA CCCAGGCCTG TGAAGGAGGC CGATGTGAGG CAGGCAGGTA CTTCCTGGGA 
ACGCCGCAGG CCGGGAGGGA GCCCTGGTGG GGCTCTCGCG CAGCCTTGCC CCTGACAAAG 
GCCTCCCCAG AAAGCAGAGA AGCCTATGAA AACAGCATGC CTCACATCGC TTCAGTCCAC 
AGGATCCATG GGCGAGGTCA TTGGGATGAA GATAGTGTGG TCAGTTCTCC AGACCCTGGG 
TCGGCCAGTG AATCAGGTGA CCGATATCGT ACTGAGCAGT ATCAAAGTAG CCCACATGAA 
CCCAGCAAAA TTGAAACTCT TATAAGAGCC ACTCAGCAAA TGATTAAAGA AGAAGAGAAC 
AGATTACAGC TAAGGAAAGC CCCCTCAGAC CAACTGGCTT CCATTAATGG GGCTGGGAAA 
AAACACTCCC TGTGTTTTGC AAACTACCAA CAGCCCCCAC CAACAGGTGA AGTCTGCCAT 
GGCTCTGCTC TTGCCAACAC TTCACCATGT GACCATATCC AGCAGAGAGA GGGAAAAATG 
TTGAGCCCCC ATGAAAATGA CTATGACAAC AGTCCCACCG CACTATCTCG GATAAGTAGT 
CCCAATTCGG ATCGCATTTC AAAATCCAGT TTGATCCTAG CTAAAGACTA TCTGCATTCG 
GATATATCTC CTCATCAGAC AGCAGGAGAC CACCCTACTG TCTCTCCAAA CTGCTTTGGC 
TCTCACCGGC AGTATTTTGA CAAGCATGCT TACACATTAA CTGGATATGC CCTGGAGCAC 
TTATATGACA GCGAAACCAT TAGAAACTAT TCCTTGGGCT GTAATGGCTC ACACTTTGAT 
GTAACTTCCC ATCTGAGGAT GCAACCAGAC CCAGCACAAG GACACAAGGG AACATCTGTT 
ATAATAACCA ACGGAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866686.1
CDS	516..2816
Translation	MKEKSKNAARTRREKENSEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRVVFPEGLGEAWGHSSRTSPLDNVGRELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVLTAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCAHHLLLVKGQVTTKYYRFLAKHGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQISASKPAFSYTSSSTPTMTDNRKGAKSRLSSSKSKSRTSPYPQYSGFHTERSESDHDSQWGGSPLTDTASPQLLDPADRPGSQHDASCAYRQFSDRSSLCYGFALDHSRLVEERHFHTQACEGGRCEAGRYFLGTPQAGREPWWGSRAALPLTKASPESREAYENSMPHIASVHRIHGRGHWDEDSVVSSPDPGSASESGDRYRTEQYQSSPHEPSKIETLIRATQQMIKEEENRLQLRKAPSDQLASINGAGKKHSLCFANYQQPPPTGEVCHGSALANTSPCDHIQQREGKMLSPHENDYDNSPTALSRISSPNSDRISKSSLILAKDYLHSDISPHQTAGDHPTVSPNCFGSHRQYFDKHAYTLTGYALEHLYDSETIRNYSLGCNGSHFDVTSHLRMQPDPAQGHKGTSVIITNGS

Target ORF information:

RefSeq Version	XM_017011197.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SIM bHLH transcription factor 1 (SIM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011197.1

ORF Insert Sequence:

ATGAAAGAAA AGTCCAAAAA TGCTGCGCGG ACTAGGAGGG AGAAGGAAAA CAGTGAATTT 
TATGAACTGG CTAAATTACT GCCTTTGCCC TCGGCTATCA CCTCGCAGCT GGACAAAGCA 
TCCATAATCA GACTCACGAC CAGCTATCTC AAAATGAGAG TGGTGTTCCC AGAAGGGCTC 
GGCGAGGCGT GGGGCCACTC AAGTCGGACC AGCCCCCTGG ACAACGTTGG CCGAGAACTG 
GGCTCCCATC TGCTCCAGAC CCTGGATGGC TTCATCTTCG TGGTAGCCCC AGATGGGAAG 
ATCATGTACA TCTCAGAGAC AGCCTCAGTC CACTTGGGTC TTTCTCAGGT AGAGCTGACC 
GGAAACAGCA TTTATGAATA CATTCACCCG GCAGACCACG ACGAGATGAC GGCGGTGCTC 
ACCGCCCATC AACCCTACCA CTCTCACTTC GTGCAGGAGT ATGAGATCGA GCGCTCCTTC 
TTCCTGAGGA TGAAGTGCGT CTTGGCCAAG CGTAACGCCG GCCTCACCTG TGGCGGCTAC 
AAGGTCATCC ACTGCAGCGG CTACTTGAAG ATCCGCCAGT ACAGCCTGGA CATGTCCCCC 
TTCGACGGCT GCTACCAAAA CGTGGGCCTG GTGGCCGTGG GCCACTCGCT GCCTCCCAGC 
GCCGTCACGG AGATCAAGCT ACACAGCAAT ATGTTTATGT TCCGCGCCAG CCTGGACATG 
AAGCTCATCT TTCTGGACTC CAGGGTGGCG GAGCTGACGG GGTACGAACC TCAGGACCTG 
ATTGAGAAGA CTCTGTACCA CCATGTGCAC GGCTGCGACA CCTTCCACCT GCGCTGCGCG 
CACCATTTGC TGCTGGTGAA GGGACAGGTG ACCACCAAGT ACTACAGGTT CCTGGCGAAA 
CACGGCGGCT GGGTATGGGT GCAGAGCTAC GCGACCATCG TGCACAACAG TCGCTCCTCC 
AGGCCACACT GTATCGTCAG CGTCAACTAT GTCCTCACAG ACACAGAATA CAAAGGGCTG 
CAGCTCTCCC TGGATCAGAT CTCAGCCTCC AAACCAGCCT TCTCCTATAC CAGCAGCTCC 
ACCCCCACCA TGACTGACAA CAGAAAGGGG GCCAAATCCC GGCTCTCCAG CTCAAAGTCA 
AAATCCAGGA CTTCCCCATA CCCTCAGTAT TCGGGATTTC ACACAGAAAG ATCGGAATCT 
GATCATGACA GCCAGTGGGG CGGAAGTCCC TTGACCGACA CGGCCTCTCC GCAGCTTCTG 
GACCCCGCCG ATAGGCCTGG CTCCCAGCAC GACGCATCGT GCGCCTACAG ACAGTTTTCG 
GACCGCAGCT CTCTCTGCTA TGGCTTTGCG CTTGACCACT CGAGGCTGGT GGAAGAGAGG 
CATTTCCATA CCCAGGCCTG TGAAGGAGGC CGATGTGAGG CAGGCAGGTA CTTCCTGGGA 
ACGCCGCAGG CCGGGAGGGA GCCCTGGTGG GGCTCTCGCG CAGCCTTGCC CCTGACAAAG 
GCCTCCCCAG AAAGCAGAGA AGCCTATGAA AACAGCATGC CTCACATCGC TTCAGTCCAC 
AGGATCCATG GGCGAGGTCA TTGGGATGAA GATAGTGTGG TCAGTTCTCC AGACCCTGGG 
TCGGCCAGTG AATCAGGTGA CCGATATCGT ACTGAGCAGT ATCAAAGTAG CCCACATGAA 
CCCAGCAAAA TTGAAACTCT TATAAGAGCC ACTCAGCAAA TGATTAAAGA AGAAGAGAAC 
AGATTACAGC TAAGGAAAGC CCCCTCAGAC CAACTGGCTT CCATTAATGG GGCTGGGAAA 
AAACACTCCC TGTGTTTTGC AAACTACCAA CAGCCCCCAC CAACAGGTGA AGTCTGCCAT 
GGCTCTGCTC TTGCCAACAC TTCACCATGT GACCATATCC AGCAGAGAGA GGGAAAAATG 
TTGAGCCCCC ATGAAAATGA CTATGACAAC AGTCCCACCG CACTATCTCG GATAAGTAGT 
CCCAATTCGG ATCGCATTTC AAAATCCAGT TTGATCCTAG CTAAAGACTA TCTGCATTCG 
GATATATCTC CTCATCAGAC AGCAGGAGAC CACCCTACTG TCTCTCCAAA CTGCTTTGGC 
TCTCACCGGC AGTATTTTGA CAAGCATGCT TACACATTAA CTGGATATGC CCTGGAGCAC 
TTATATGACA GCGAAACCAT TAGAAACTAT TCCTTGGGCT GTAATGGCTC ACACTTTGAT 
GTAACTTCCC ATCTGAGGAT GCAACCAGAC CCAGCACAAG GACACAAGGG AACATCTGTT 
ATAATAACCA ACGGAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIM1 ( XM_005267100.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005267157.1 Homo sapiens SIM bHLH transcription factor 1 (SIM1), transcript variant X1, mRNA.

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CloneID	OHu18964
Clone ID Related Accession (Same CDS sequence)	NM_001374769.1 , XM_017011197.1 , NM_005068.2 , XM_005267100.2 , NM_005068.3
Accession Version	XM_005267100.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	single-minded homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005267100.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGAAAGAAA AGTCCAAAAA TGCTGCGCGG ACTAGGAGGG AGAAGGAAAA CAGTGAATTT 
TATGAACTGG CTAAATTACT GCCTTTGCCC TCGGCTATCA CCTCGCAGCT GGACAAAGCA 
TCCATAATCA GACTCACGAC CAGCTATCTC AAAATGAGAG TGGTGTTCCC AGAAGGGCTC 
GGCGAGGCGT GGGGCCACTC AAGTCGGACC AGCCCCCTGG ACAACGTTGG CCGAGAACTG 
GGCTCCCATC TGCTCCAGAC CCTGGATGGC TTCATCTTCG TGGTAGCCCC AGATGGGAAG 
ATCATGTACA TCTCAGAGAC AGCCTCAGTC CACTTGGGTC TTTCTCAGGT AGAGCTGACC 
GGAAACAGCA TTTATGAATA CATTCACCCG GCAGACCACG ACGAGATGAC GGCGGTGCTC 
ACCGCCCATC AACCCTACCA CTCTCACTTC GTGCAGGAGT ATGAGATCGA GCGCTCCTTC 
TTCCTGAGGA TGAAGTGCGT CTTGGCCAAG CGTAACGCCG GCCTCACCTG TGGCGGCTAC 
AAGGTCATCC ACTGCAGCGG CTACTTGAAG ATCCGCCAGT ACAGCCTGGA CATGTCCCCC 
TTCGACGGCT GCTACCAAAA CGTGGGCCTG GTGGCCGTGG GCCACTCGCT GCCTCCCAGC 
GCCGTCACGG AGATCAAGCT ACACAGCAAT ATGTTTATGT TCCGCGCCAG CCTGGACATG 
AAGCTCATCT TTCTGGACTC CAGGGTGGCG GAGCTGACGG GGTACGAACC TCAGGACCTG 
ATTGAGAAGA CTCTGTACCA CCATGTGCAC GGCTGCGACA CCTTCCACCT GCGCTGCGCG 
CACCATTTGC TGCTGGTGAA GGGACAGGTG ACCACCAAGT ACTACAGGTT CCTGGCGAAA 
CACGGCGGCT GGGTATGGGT GCAGAGCTAC GCGACCATCG TGCACAACAG TCGCTCCTCC 
AGGCCACACT GTATCGTCAG CGTCAACTAT GTCCTCACAG ACACAGAATA CAAAGGGCTG 
CAGCTCTCCC TGGATCAGAT CTCAGCCTCC AAACCAGCCT TCTCCTATAC CAGCAGCTCC 
ACCCCCACCA TGACTGACAA CAGAAAGGGG GCCAAATCCC GGCTCTCCAG CTCAAAGTCA 
AAATCCAGGA CTTCCCCATA CCCTCAGTAT TCGGGATTTC ACACAGAAAG ATCGGAATCT 
GATCATGACA GCCAGTGGGG CGGAAGTCCC TTGACCGACA CGGCCTCTCC GCAGCTTCTG 
GACCCCGCCG ATAGGCCTGG CTCCCAGCAC GACGCATCGT GCGCCTACAG ACAGTTTTCG 
GACCGCAGCT CTCTCTGCTA TGGCTTTGCG CTTGACCACT CGAGGCTGGT GGAAGAGAGG 
CATTTCCATA CCCAGGCCTG TGAAGGAGGC CGATGTGAGG CAGGCAGGTA CTTCCTGGGA 
ACGCCGCAGG CCGGGAGGGA GCCCTGGTGG GGCTCTCGCG CAGCCTTGCC CCTGACAAAG 
GCCTCCCCAG AAAGCAGAGA AGCCTATGAA AACAGCATGC CTCACATCGC TTCAGTCCAC 
AGGATCCATG GGCGAGGTCA TTGGGATGAA GATAGTGTGG TCAGTTCTCC AGACCCTGGG 
TCGGCCAGTG AATCAGGTGA CCGATATCGT ACTGAGCAGT ATCAAAGTAG CCCACATGAA 
CCCAGCAAAA TTGAAACTCT TATAAGAGCC ACTCAGCAAA TGATTAAAGA AGAAGAGAAC 
AGATTACAGC TAAGGAAAGC CCCCTCAGAC CAACTGGCTT CCATTAATGG GGCTGGGAAA 
AAACACTCCC TGTGTTTTGC AAACTACCAA CAGCCCCCAC CAACAGGTGA AGTCTGCCAT 
GGCTCTGCTC TTGCCAACAC TTCACCATGT GACCATATCC AGCAGAGAGA GGGAAAAATG 
TTGAGCCCCC ATGAAAATGA CTATGACAAC AGTCCCACCG CACTATCTCG GATAAGTAGT 
CCCAATTCGG ATCGCATTTC AAAATCCAGT TTGATCCTAG CTAAAGACTA TCTGCATTCG 
GATATATCTC CTCATCAGAC AGCAGGAGAC CACCCTACTG TCTCTCCAAA CTGCTTTGGC 
TCTCACCGGC AGTATTTTGA CAAGCATGCT TACACATTAA CTGGATATGC CCTGGAGCAC 
TTATATGACA GCGAAACCAT TAGAAACTAT TCCTTGGGCT GTAATGGCTC ACACTTTGAT 
GTAACTTCCC ATCTGAGGAT GCAACCAGAC CCAGCACAAG GACACAAGGG AACATCTGTT 
ATAATAACCA ACGGAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005267157.1
CDS	784..3084
Translation	MKEKSKNAARTRREKENSEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRVVFPEGLGEAWGHSSRTSPLDNVGRELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVLTAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCAHHLLLVKGQVTTKYYRFLAKHGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQISASKPAFSYTSSSTPTMTDNRKGAKSRLSSSKSKSRTSPYPQYSGFHTERSESDHDSQWGGSPLTDTASPQLLDPADRPGSQHDASCAYRQFSDRSSLCYGFALDHSRLVEERHFHTQACEGGRCEAGRYFLGTPQAGREPWWGSRAALPLTKASPESREAYENSMPHIASVHRIHGRGHWDEDSVVSSPDPGSASESGDRYRTEQYQSSPHEPSKIETLIRATQQMIKEEENRLQLRKAPSDQLASINGAGKKHSLCFANYQQPPPTGEVCHGSALANTSPCDHIQQREGKMLSPHENDYDNSPTALSRISSPNSDRISKSSLILAKDYLHSDISPHQTAGDHPTVSPNCFGSHRQYFDKHAYTLTGYALEHLYDSETIRNYSLGCNGSHFDVTSHLRMQPDPAQGHKGTSVIITNGS

Target ORF information:

RefSeq Version	XM_005267100.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SIM bHLH transcription factor 1 (SIM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005267100.2

ORF Insert Sequence:

ATGAAAGAAA AGTCCAAAAA TGCTGCGCGG ACTAGGAGGG AGAAGGAAAA CAGTGAATTT 
TATGAACTGG CTAAATTACT GCCTTTGCCC TCGGCTATCA CCTCGCAGCT GGACAAAGCA 
TCCATAATCA GACTCACGAC CAGCTATCTC AAAATGAGAG TGGTGTTCCC AGAAGGGCTC 
GGCGAGGCGT GGGGCCACTC AAGTCGGACC AGCCCCCTGG ACAACGTTGG CCGAGAACTG 
GGCTCCCATC TGCTCCAGAC CCTGGATGGC TTCATCTTCG TGGTAGCCCC AGATGGGAAG 
ATCATGTACA TCTCAGAGAC AGCCTCAGTC CACTTGGGTC TTTCTCAGGT AGAGCTGACC 
GGAAACAGCA TTTATGAATA CATTCACCCG GCAGACCACG ACGAGATGAC GGCGGTGCTC 
ACCGCCCATC AACCCTACCA CTCTCACTTC GTGCAGGAGT ATGAGATCGA GCGCTCCTTC 
TTCCTGAGGA TGAAGTGCGT CTTGGCCAAG CGTAACGCCG GCCTCACCTG TGGCGGCTAC 
AAGGTCATCC ACTGCAGCGG CTACTTGAAG ATCCGCCAGT ACAGCCTGGA CATGTCCCCC 
TTCGACGGCT GCTACCAAAA CGTGGGCCTG GTGGCCGTGG GCCACTCGCT GCCTCCCAGC 
GCCGTCACGG AGATCAAGCT ACACAGCAAT ATGTTTATGT TCCGCGCCAG CCTGGACATG 
AAGCTCATCT TTCTGGACTC CAGGGTGGCG GAGCTGACGG GGTACGAACC TCAGGACCTG 
ATTGAGAAGA CTCTGTACCA CCATGTGCAC GGCTGCGACA CCTTCCACCT GCGCTGCGCG 
CACCATTTGC TGCTGGTGAA GGGACAGGTG ACCACCAAGT ACTACAGGTT CCTGGCGAAA 
CACGGCGGCT GGGTATGGGT GCAGAGCTAC GCGACCATCG TGCACAACAG TCGCTCCTCC 
AGGCCACACT GTATCGTCAG CGTCAACTAT GTCCTCACAG ACACAGAATA CAAAGGGCTG 
CAGCTCTCCC TGGATCAGAT CTCAGCCTCC AAACCAGCCT TCTCCTATAC CAGCAGCTCC 
ACCCCCACCA TGACTGACAA CAGAAAGGGG GCCAAATCCC GGCTCTCCAG CTCAAAGTCA 
AAATCCAGGA CTTCCCCATA CCCTCAGTAT TCGGGATTTC ACACAGAAAG ATCGGAATCT 
GATCATGACA GCCAGTGGGG CGGAAGTCCC TTGACCGACA CGGCCTCTCC GCAGCTTCTG 
GACCCCGCCG ATAGGCCTGG CTCCCAGCAC GACGCATCGT GCGCCTACAG ACAGTTTTCG 
GACCGCAGCT CTCTCTGCTA TGGCTTTGCG CTTGACCACT CGAGGCTGGT GGAAGAGAGG 
CATTTCCATA CCCAGGCCTG TGAAGGAGGC CGATGTGAGG CAGGCAGGTA CTTCCTGGGA 
ACGCCGCAGG CCGGGAGGGA GCCCTGGTGG GGCTCTCGCG CAGCCTTGCC CCTGACAAAG 
GCCTCCCCAG AAAGCAGAGA AGCCTATGAA AACAGCATGC CTCACATCGC TTCAGTCCAC 
AGGATCCATG GGCGAGGTCA TTGGGATGAA GATAGTGTGG TCAGTTCTCC AGACCCTGGG 
TCGGCCAGTG AATCAGGTGA CCGATATCGT ACTGAGCAGT ATCAAAGTAG CCCACATGAA 
CCCAGCAAAA TTGAAACTCT TATAAGAGCC ACTCAGCAAA TGATTAAAGA AGAAGAGAAC 
AGATTACAGC TAAGGAAAGC CCCCTCAGAC CAACTGGCTT CCATTAATGG GGCTGGGAAA 
AAACACTCCC TGTGTTTTGC AAACTACCAA CAGCCCCCAC CAACAGGTGA AGTCTGCCAT 
GGCTCTGCTC TTGCCAACAC TTCACCATGT GACCATATCC AGCAGAGAGA GGGAAAAATG 
TTGAGCCCCC ATGAAAATGA CTATGACAAC AGTCCCACCG CACTATCTCG GATAAGTAGT 
CCCAATTCGG ATCGCATTTC AAAATCCAGT TTGATCCTAG CTAAAGACTA TCTGCATTCG 
GATATATCTC CTCATCAGAC AGCAGGAGAC CACCCTACTG TCTCTCCAAA CTGCTTTGGC 
TCTCACCGGC AGTATTTTGA CAAGCATGCT TACACATTAA CTGGATATGC CCTGGAGCAC 
TTATATGACA GCGAAACCAT TAGAAACTAT TCCTTGGGCT GTAATGGCTC ACACTTTGAT 
GTAACTTCCC ATCTGAGGAT GCAACCAGAC CCAGCACAAG GACACAAGGG AACATCTGTT 
ATAATAACCA ACGGAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIM1 ( NM_001374769.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001361698.1 Homo sapiens SIM bHLH transcription factor 1 (SIM1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu18964
Clone ID Related Accession (Same CDS sequence)	NM_001374769.1 , XM_017011197.1 , NM_005068.2 , XM_005267100.2 , NM_005068.3
Accession Version	NM_001374769.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	single-minded homolog 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Z86062.1 and AL121948.8. On Oct 11, 2019 this sequence version replaced XM_017011197.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U70212.1 [ECO:0000331] ##Evidence-Data-END##

ATGAAAGAAA AGTCCAAAAA TGCTGCGCGG ACTAGGAGGG AGAAGGAAAA CAGTGAATTT 
TATGAACTGG CTAAATTACT GCCTTTGCCC TCGGCTATCA CCTCGCAGCT GGACAAAGCA 
TCCATAATCA GACTCACGAC CAGCTATCTC AAAATGAGAG TGGTGTTCCC AGAAGGGCTC 
GGCGAGGCGT GGGGCCACTC AAGTCGGACC AGCCCCCTGG ACAACGTTGG CCGAGAACTG 
GGCTCCCATC TGCTCCAGAC CCTGGATGGC TTCATCTTCG TGGTAGCCCC AGATGGGAAG 
ATCATGTACA TCTCAGAGAC AGCCTCAGTC CACTTGGGTC TTTCTCAGGT AGAGCTGACC 
GGAAACAGCA TTTATGAATA CATTCACCCG GCAGACCACG ACGAGATGAC GGCGGTGCTC 
ACCGCCCATC AACCCTACCA CTCTCACTTC GTGCAGGAGT ATGAGATCGA GCGCTCCTTC 
TTCCTGAGGA TGAAGTGCGT CTTGGCCAAG CGTAACGCCG GCCTCACCTG TGGCGGCTAC 
AAGGTCATCC ACTGCAGCGG CTACTTGAAG ATCCGCCAGT ACAGCCTGGA CATGTCCCCC 
TTCGACGGCT GCTACCAAAA CGTGGGCCTG GTGGCCGTGG GCCACTCGCT GCCTCCCAGC 
GCCGTCACGG AGATCAAGCT ACACAGCAAT ATGTTTATGT TCCGCGCCAG CCTGGACATG 
AAGCTCATCT TTCTGGACTC CAGGGTGGCG GAGCTGACGG GGTACGAACC TCAGGACCTG 
ATTGAGAAGA CTCTGTACCA CCATGTGCAC GGCTGCGACA CCTTCCACCT GCGCTGCGCG 
CACCATTTGC TGCTGGTGAA GGGACAGGTG ACCACCAAGT ACTACAGGTT CCTGGCGAAA 
CACGGCGGCT GGGTATGGGT GCAGAGCTAC GCGACCATCG TGCACAACAG TCGCTCCTCC 
AGGCCACACT GTATCGTCAG CGTCAACTAT GTCCTCACAG ACACAGAATA CAAAGGGCTG 
CAGCTCTCCC TGGATCAGAT CTCAGCCTCC AAACCAGCCT TCTCCTATAC CAGCAGCTCC 
ACCCCCACCA TGACTGACAA CAGAAAGGGG GCCAAATCCC GGCTCTCCAG CTCAAAGTCA 
AAATCCAGGA CTTCCCCATA CCCTCAGTAT TCGGGATTTC ACACAGAAAG ATCGGAATCT 
GATCATGACA GCCAGTGGGG CGGAAGTCCC TTGACCGACA CGGCCTCTCC GCAGCTTCTG 
GACCCCGCCG ATAGGCCTGG CTCCCAGCAC GACGCATCGT GCGCCTACAG ACAGTTTTCG 
GACCGCAGCT CTCTCTGCTA TGGCTTTGCG CTTGACCACT CGAGGCTGGT GGAAGAGAGG 
CATTTCCATA CCCAGGCCTG TGAAGGAGGC CGATGTGAGG CAGGCAGGTA CTTCCTGGGA 
ACGCCGCAGG CCGGGAGGGA GCCCTGGTGG GGCTCTCGCG CAGCCTTGCC CCTGACAAAG 
GCCTCCCCAG AAAGCAGAGA AGCCTATGAA AACAGCATGC CTCACATCGC TTCAGTCCAC 
AGGATCCATG GGCGAGGTCA TTGGGATGAA GATAGTGTGG TCAGTTCTCC AGACCCTGGG 
TCGGCCAGTG AATCAGGTGA CCGATATCGT ACTGAGCAGT ATCAAAGTAG CCCACATGAA 
CCCAGCAAAA TTGAAACTCT TATAAGAGCC ACTCAGCAAA TGATTAAAGA AGAAGAGAAC 
AGATTACAGC TAAGGAAAGC CCCCTCAGAC CAACTGGCTT CCATTAATGG GGCTGGGAAA 
AAACACTCCC TGTGTTTTGC AAACTACCAA CAGCCCCCAC CAACAGGTGA AGTCTGCCAT 
GGCTCTGCTC TTGCCAACAC TTCACCATGT GACCATATCC AGCAGAGAGA GGGAAAAATG 
TTGAGCCCCC ATGAAAATGA CTATGACAAC AGTCCCACCG CACTATCTCG GATAAGTAGT 
CCCAATTCGG ATCGCATTTC AAAATCCAGT TTGATCCTAG CTAAAGACTA TCTGCATTCG 
GATATATCTC CTCATCAGAC AGCAGGAGAC CACCCTACTG TCTCTCCAAA CTGCTTTGGC 
TCTCACCGGC AGTATTTTGA CAAGCATGCT TACACATTAA CTGGATATGC CCTGGAGCAC 
TTATATGACA GCGAAACCAT TAGAAACTAT TCCTTGGGCT GTAATGGCTC ACACTTTGAT 
GTAACTTCCC ATCTGAGGAT GCAACCAGAC CCAGCACAAG GACACAAGGG AACATCTGTT 
ATAATAACCA ACGGAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361698.1
CDS	529..2829
Translation	MKEKSKNAARTRREKENSEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRVVFPEGLGEAWGHSSRTSPLDNVGRELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVLTAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCAHHLLLVKGQVTTKYYRFLAKHGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQISASKPAFSYTSSSTPTMTDNRKGAKSRLSSSKSKSRTSPYPQYSGFHTERSESDHDSQWGGSPLTDTASPQLLDPADRPGSQHDASCAYRQFSDRSSLCYGFALDHSRLVEERHFHTQACEGGRCEAGRYFLGTPQAGREPWWGSRAALPLTKASPESREAYENSMPHIASVHRIHGRGHWDEDSVVSSPDPGSASESGDRYRTEQYQSSPHEPSKIETLIRATQQMIKEEENRLQLRKAPSDQLASINGAGKKHSLCFANYQQPPPTGEVCHGSALANTSPCDHIQQREGKMLSPHENDYDNSPTALSRISSPNSDRISKSSLILAKDYLHSDISPHQTAGDHPTVSPNCFGSHRQYFDKHAYTLTGYALEHLYDSETIRNYSLGCNGSHFDVTSHLRMQPDPAQGHKGTSVIITNGS

Target ORF information:

RefSeq Version	NM_001374769.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SIM bHLH transcription factor 1 (SIM1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374769.1

ORF Insert Sequence:

ATGAAAGAAA AGTCCAAAAA TGCTGCGCGG ACTAGGAGGG AGAAGGAAAA CAGTGAATTT 
TATGAACTGG CTAAATTACT GCCTTTGCCC TCGGCTATCA CCTCGCAGCT GGACAAAGCA 
TCCATAATCA GACTCACGAC CAGCTATCTC AAAATGAGAG TGGTGTTCCC AGAAGGGCTC 
GGCGAGGCGT GGGGCCACTC AAGTCGGACC AGCCCCCTGG ACAACGTTGG CCGAGAACTG 
GGCTCCCATC TGCTCCAGAC CCTGGATGGC TTCATCTTCG TGGTAGCCCC AGATGGGAAG 
ATCATGTACA TCTCAGAGAC AGCCTCAGTC CACTTGGGTC TTTCTCAGGT AGAGCTGACC 
GGAAACAGCA TTTATGAATA CATTCACCCG GCAGACCACG ACGAGATGAC GGCGGTGCTC 
ACCGCCCATC AACCCTACCA CTCTCACTTC GTGCAGGAGT ATGAGATCGA GCGCTCCTTC 
TTCCTGAGGA TGAAGTGCGT CTTGGCCAAG CGTAACGCCG GCCTCACCTG TGGCGGCTAC 
AAGGTCATCC ACTGCAGCGG CTACTTGAAG ATCCGCCAGT ACAGCCTGGA CATGTCCCCC 
TTCGACGGCT GCTACCAAAA CGTGGGCCTG GTGGCCGTGG GCCACTCGCT GCCTCCCAGC 
GCCGTCACGG AGATCAAGCT ACACAGCAAT ATGTTTATGT TCCGCGCCAG CCTGGACATG 
AAGCTCATCT TTCTGGACTC CAGGGTGGCG GAGCTGACGG GGTACGAACC TCAGGACCTG 
ATTGAGAAGA CTCTGTACCA CCATGTGCAC GGCTGCGACA CCTTCCACCT GCGCTGCGCG 
CACCATTTGC TGCTGGTGAA GGGACAGGTG ACCACCAAGT ACTACAGGTT CCTGGCGAAA 
CACGGCGGCT GGGTATGGGT GCAGAGCTAC GCGACCATCG TGCACAACAG TCGCTCCTCC 
AGGCCACACT GTATCGTCAG CGTCAACTAT GTCCTCACAG ACACAGAATA CAAAGGGCTG 
CAGCTCTCCC TGGATCAGAT CTCAGCCTCC AAACCAGCCT TCTCCTATAC CAGCAGCTCC 
ACCCCCACCA TGACTGACAA CAGAAAGGGG GCCAAATCCC GGCTCTCCAG CTCAAAGTCA 
AAATCCAGGA CTTCCCCATA CCCTCAGTAT TCGGGATTTC ACACAGAAAG ATCGGAATCT 
GATCATGACA GCCAGTGGGG CGGAAGTCCC TTGACCGACA CGGCCTCTCC GCAGCTTCTG 
GACCCCGCCG ATAGGCCTGG CTCCCAGCAC GACGCATCGT GCGCCTACAG ACAGTTTTCG 
GACCGCAGCT CTCTCTGCTA TGGCTTTGCG CTTGACCACT CGAGGCTGGT GGAAGAGAGG 
CATTTCCATA CCCAGGCCTG TGAAGGAGGC CGATGTGAGG CAGGCAGGTA CTTCCTGGGA 
ACGCCGCAGG CCGGGAGGGA GCCCTGGTGG GGCTCTCGCG CAGCCTTGCC CCTGACAAAG 
GCCTCCCCAG AAAGCAGAGA AGCCTATGAA AACAGCATGC CTCACATCGC TTCAGTCCAC 
AGGATCCATG GGCGAGGTCA TTGGGATGAA GATAGTGTGG TCAGTTCTCC AGACCCTGGG 
TCGGCCAGTG AATCAGGTGA CCGATATCGT ACTGAGCAGT ATCAAAGTAG CCCACATGAA 
CCCAGCAAAA TTGAAACTCT TATAAGAGCC ACTCAGCAAA TGATTAAAGA AGAAGAGAAC 
AGATTACAGC TAAGGAAAGC CCCCTCAGAC CAACTGGCTT CCATTAATGG GGCTGGGAAA 
AAACACTCCC TGTGTTTTGC AAACTACCAA CAGCCCCCAC CAACAGGTGA AGTCTGCCAT 
GGCTCTGCTC TTGCCAACAC TTCACCATGT GACCATATCC AGCAGAGAGA GGGAAAAATG 
TTGAGCCCCC ATGAAAATGA CTATGACAAC AGTCCCACCG CACTATCTCG GATAAGTAGT 
CCCAATTCGG ATCGCATTTC AAAATCCAGT TTGATCCTAG CTAAAGACTA TCTGCATTCG 
GATATATCTC CTCATCAGAC AGCAGGAGAC CACCCTACTG TCTCTCCAAA CTGCTTTGGC 
TCTCACCGGC AGTATTTTGA CAAGCATGCT TACACATTAA CTGGATATGC CCTGGAGCAC 
TTATATGACA GCGAAACCAT TAGAAACTAT TCCTTGGGCT GTAATGGCTC ACACTTTGAT 
GTAACTTCCC ATCTGAGGAT GCAACCAGAC CCAGCACAAG GACACAAGGG AACATCTGTT 
ATAATAACCA ACGGAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIM1 ( NM_005068.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005059.2 Homo sapiens SIM bHLH transcription factor 1 (SIM1), transcript variant 1, mRNA.

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CloneID	OHu18964
Clone ID Related Accession (Same CDS sequence)	NM_001374769.1 , XM_017011197.1 , NM_005068.2 , XM_005267100.2 , NM_005068.3
Accession Version	NM_005068.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Homo sapiens(human)
Product	single-minded homolog 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Z86062.1 and AL121948.8. On or before Oct 11, 2019 this sequence version replaced XM_005267100.2, NM_005068.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK308090.1, DRR138518.34632.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGAAAGAAA AGTCCAAAAA TGCTGCGCGG ACTAGGAGGG AGAAGGAAAA CAGTGAATTT 
TATGAACTGG CTAAATTACT GCCTTTGCCC TCGGCTATCA CCTCGCAGCT GGACAAAGCA 
TCCATAATCA GACTCACGAC CAGCTATCTC AAAATGAGAG TGGTGTTCCC AGAAGGGCTC 
GGCGAGGCGT GGGGCCACTC AAGTCGGACC AGCCCCCTGG ACAACGTTGG CCGAGAACTG 
GGCTCCCATC TGCTCCAGAC CCTGGATGGC TTCATCTTCG TGGTAGCCCC AGATGGGAAG 
ATCATGTACA TCTCAGAGAC AGCCTCAGTC CACTTGGGTC TTTCTCAGGT AGAGCTGACC 
GGAAACAGCA TTTATGAATA CATTCACCCG GCAGACCACG ACGAGATGAC GGCGGTGCTC 
ACCGCCCATC AACCCTACCA CTCTCACTTC GTGCAGGAGT ATGAGATCGA GCGCTCCTTC 
TTCCTGAGGA TGAAGTGCGT CTTGGCCAAG CGTAACGCCG GCCTCACCTG TGGCGGCTAC 
AAGGTCATCC ACTGCAGCGG CTACTTGAAG ATCCGCCAGT ACAGCCTGGA CATGTCCCCC 
TTCGACGGCT GCTACCAAAA CGTGGGCCTG GTGGCCGTGG GCCACTCGCT GCCTCCCAGC 
GCCGTCACGG AGATCAAGCT ACACAGCAAT ATGTTTATGT TCCGCGCCAG CCTGGACATG 
AAGCTCATCT TTCTGGACTC CAGGGTGGCG GAGCTGACGG GGTACGAACC TCAGGACCTG 
ATTGAGAAGA CTCTGTACCA CCATGTGCAC GGCTGCGACA CCTTCCACCT GCGCTGCGCG 
CACCATTTGC TGCTGGTGAA GGGACAGGTG ACCACCAAGT ACTACAGGTT CCTGGCGAAA 
CACGGCGGCT GGGTATGGGT GCAGAGCTAC GCGACCATCG TGCACAACAG TCGCTCCTCC 
AGGCCACACT GTATCGTCAG CGTCAACTAT GTCCTCACAG ACACAGAATA CAAAGGGCTG 
CAGCTCTCCC TGGATCAGAT CTCAGCCTCC AAACCAGCCT TCTCCTATAC CAGCAGCTCC 
ACCCCCACCA TGACTGACAA CAGAAAGGGG GCCAAATCCC GGCTCTCCAG CTCAAAGTCA 
AAATCCAGGA CTTCCCCATA CCCTCAGTAT TCGGGATTTC ACACAGAAAG ATCGGAATCT 
GATCATGACA GCCAGTGGGG CGGAAGTCCC TTGACCGACA CGGCCTCTCC GCAGCTTCTG 
GACCCCGCCG ATAGGCCTGG CTCCCAGCAC GACGCATCGT GCGCCTACAG ACAGTTTTCG 
GACCGCAGCT CTCTCTGCTA TGGCTTTGCG CTTGACCACT CGAGGCTGGT GGAAGAGAGG 
CATTTCCATA CCCAGGCCTG TGAAGGAGGC CGATGTGAGG CAGGCAGGTA CTTCCTGGGA 
ACGCCGCAGG CCGGGAGGGA GCCCTGGTGG GGCTCTCGCG CAGCCTTGCC CCTGACAAAG 
GCCTCCCCAG AAAGCAGAGA AGCCTATGAA AACAGCATGC CTCACATCGC TTCAGTCCAC 
AGGATCCATG GGCGAGGTCA TTGGGATGAA GATAGTGTGG TCAGTTCTCC AGACCCTGGG 
TCGGCCAGTG AATCAGGTGA CCGATATCGT ACTGAGCAGT ATCAAAGTAG CCCACATGAA 
CCCAGCAAAA TTGAAACTCT TATAAGAGCC ACTCAGCAAA TGATTAAAGA AGAAGAGAAC 
AGATTACAGC TAAGGAAAGC CCCCTCAGAC CAACTGGCTT CCATTAATGG GGCTGGGAAA 
AAACACTCCC TGTGTTTTGC AAACTACCAA CAGCCCCCAC CAACAGGTGA AGTCTGCCAT 
GGCTCTGCTC TTGCCAACAC TTCACCATGT GACCATATCC AGCAGAGAGA GGGAAAAATG 
TTGAGCCCCC ATGAAAATGA CTATGACAAC AGTCCCACCG CACTATCTCG GATAAGTAGT 
CCCAATTCGG ATCGCATTTC AAAATCCAGT TTGATCCTAG CTAAAGACTA TCTGCATTCG 
GATATATCTC CTCATCAGAC AGCAGGAGAC CACCCTACTG TCTCTCCAAA CTGCTTTGGC 
TCTCACCGGC AGTATTTTGA CAAGCATGCT TACACATTAA CTGGATATGC CCTGGAGCAC 
TTATATGACA GCGAAACCAT TAGAAACTAT TCCTTGGGCT GTAATGGCTC ACACTTTGAT 
GTAACTTCCC ATCTGAGGAT GCAACCAGAC CCAGCACAAG GACACAAGGG AACATCTGTT 
ATAATAACCA ACGGAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005059.2
CDS	776..3076
Translation	MKEKSKNAARTRREKENSEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRVVFPEGLGEAWGHSSRTSPLDNVGRELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVLTAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCAHHLLLVKGQVTTKYYRFLAKHGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQISASKPAFSYTSSSTPTMTDNRKGAKSRLSSSKSKSRTSPYPQYSGFHTERSESDHDSQWGGSPLTDTASPQLLDPADRPGSQHDASCAYRQFSDRSSLCYGFALDHSRLVEERHFHTQACEGGRCEAGRYFLGTPQAGREPWWGSRAALPLTKASPESREAYENSMPHIASVHRIHGRGHWDEDSVVSSPDPGSASESGDRYRTEQYQSSPHEPSKIETLIRATQQMIKEEENRLQLRKAPSDQLASINGAGKKHSLCFANYQQPPPTGEVCHGSALANTSPCDHIQQREGKMLSPHENDYDNSPTALSRISSPNSDRISKSSLILAKDYLHSDISPHQTAGDHPTVSPNCFGSHRQYFDKHAYTLTGYALEHLYDSETIRNYSLGCNGSHFDVTSHLRMQPDPAQGHKGTSVIITNGS

Target ORF information:

RefSeq Version	NM_005068.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SIM bHLH transcription factor 1 (SIM1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005068.3

ORF Insert Sequence:

ATGAAAGAAA AGTCCAAAAA TGCTGCGCGG ACTAGGAGGG AGAAGGAAAA CAGTGAATTT 
TATGAACTGG CTAAATTACT GCCTTTGCCC TCGGCTATCA CCTCGCAGCT GGACAAAGCA 
TCCATAATCA GACTCACGAC CAGCTATCTC AAAATGAGAG TGGTGTTCCC AGAAGGGCTC 
GGCGAGGCGT GGGGCCACTC AAGTCGGACC AGCCCCCTGG ACAACGTTGG CCGAGAACTG 
GGCTCCCATC TGCTCCAGAC CCTGGATGGC TTCATCTTCG TGGTAGCCCC AGATGGGAAG 
ATCATGTACA TCTCAGAGAC AGCCTCAGTC CACTTGGGTC TTTCTCAGGT AGAGCTGACC 
GGAAACAGCA TTTATGAATA CATTCACCCG GCAGACCACG ACGAGATGAC GGCGGTGCTC 
ACCGCCCATC AACCCTACCA CTCTCACTTC GTGCAGGAGT ATGAGATCGA GCGCTCCTTC 
TTCCTGAGGA TGAAGTGCGT CTTGGCCAAG CGTAACGCCG GCCTCACCTG TGGCGGCTAC 
AAGGTCATCC ACTGCAGCGG CTACTTGAAG ATCCGCCAGT ACAGCCTGGA CATGTCCCCC 
TTCGACGGCT GCTACCAAAA CGTGGGCCTG GTGGCCGTGG GCCACTCGCT GCCTCCCAGC 
GCCGTCACGG AGATCAAGCT ACACAGCAAT ATGTTTATGT TCCGCGCCAG CCTGGACATG 
AAGCTCATCT TTCTGGACTC CAGGGTGGCG GAGCTGACGG GGTACGAACC TCAGGACCTG 
ATTGAGAAGA CTCTGTACCA CCATGTGCAC GGCTGCGACA CCTTCCACCT GCGCTGCGCG 
CACCATTTGC TGCTGGTGAA GGGACAGGTG ACCACCAAGT ACTACAGGTT CCTGGCGAAA 
CACGGCGGCT GGGTATGGGT GCAGAGCTAC GCGACCATCG TGCACAACAG TCGCTCCTCC 
AGGCCACACT GTATCGTCAG CGTCAACTAT GTCCTCACAG ACACAGAATA CAAAGGGCTG 
CAGCTCTCCC TGGATCAGAT CTCAGCCTCC AAACCAGCCT TCTCCTATAC CAGCAGCTCC 
ACCCCCACCA TGACTGACAA CAGAAAGGGG GCCAAATCCC GGCTCTCCAG CTCAAAGTCA 
AAATCCAGGA CTTCCCCATA CCCTCAGTAT TCGGGATTTC ACACAGAAAG ATCGGAATCT 
GATCATGACA GCCAGTGGGG CGGAAGTCCC TTGACCGACA CGGCCTCTCC GCAGCTTCTG 
GACCCCGCCG ATAGGCCTGG CTCCCAGCAC GACGCATCGT GCGCCTACAG ACAGTTTTCG 
GACCGCAGCT CTCTCTGCTA TGGCTTTGCG CTTGACCACT CGAGGCTGGT GGAAGAGAGG 
CATTTCCATA CCCAGGCCTG TGAAGGAGGC CGATGTGAGG CAGGCAGGTA CTTCCTGGGA 
ACGCCGCAGG CCGGGAGGGA GCCCTGGTGG GGCTCTCGCG CAGCCTTGCC CCTGACAAAG 
GCCTCCCCAG AAAGCAGAGA AGCCTATGAA AACAGCATGC CTCACATCGC TTCAGTCCAC 
AGGATCCATG GGCGAGGTCA TTGGGATGAA GATAGTGTGG TCAGTTCTCC AGACCCTGGG 
TCGGCCAGTG AATCAGGTGA CCGATATCGT ACTGAGCAGT ATCAAAGTAG CCCACATGAA 
CCCAGCAAAA TTGAAACTCT TATAAGAGCC ACTCAGCAAA TGATTAAAGA AGAAGAGAAC 
AGATTACAGC TAAGGAAAGC CCCCTCAGAC CAACTGGCTT CCATTAATGG GGCTGGGAAA 
AAACACTCCC TGTGTTTTGC AAACTACCAA CAGCCCCCAC CAACAGGTGA AGTCTGCCAT 
GGCTCTGCTC TTGCCAACAC TTCACCATGT GACCATATCC AGCAGAGAGA GGGAAAAATG 
TTGAGCCCCC ATGAAAATGA CTATGACAAC AGTCCCACCG CACTATCTCG GATAAGTAGT 
CCCAATTCGG ATCGCATTTC AAAATCCAGT TTGATCCTAG CTAAAGACTA TCTGCATTCG 
GATATATCTC CTCATCAGAC AGCAGGAGAC CACCCTACTG TCTCTCCAAA CTGCTTTGGC 
TCTCACCGGC AGTATTTTGA CAAGCATGCT TACACATTAA CTGGATATGC CCTGGAGCAC 
TTATATGACA GCGAAACCAT TAGAAACTAT TCCTTGGGCT GTAATGGCTC ACACTTTGAT 
GTAACTTCCC ATCTGAGGAT GCAACCAGAC CCAGCACAAG GACACAAGGG AACATCTGTT 
ATAATAACCA ACGGAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Aberrant single-minded homolog 1 methylation as a potential biomarker for cervical cancer.
Diagnostic cytopathology46(1)15-21(2018 Jan)
Kim HJ,Kim CY,Jin J,Bae MK,Kim YH,Ju W,Kim YH,Kim SC

Copy number variations in "classical" obesity candidate genes are not frequently associated with severe early-onset obesity in children.
Journal of pediatric endocrinology & metabolism : JPEM30(5)507-515(2017 May)
Windholz J,Kovacs P,Schlicke M,Franke C,Mahajan A,Morris AP,Lemke JR,Klammt J,Kiess W,Sch?neberg T,Pf?ffle R,K?rner A

Genetic analysis of single-minded 1 gene in early-onset severely obese children and adolescents.
PloS one12(5)e0177222(2017)
Stanikova D,Buzga M,Krumpolec P,Skopkova M,Surova M,Ukropcova B,Ticha L,Petrasova M,Gabcova D,Huckova M,Piskorova L,Bozensky J,Mokan M,Ukropec J,Zavacka I,Klimes I,Stanik J,Gasperikova D

Copy number variation (CNV) analysis and mutation analysis of the 6q14.1-6q16.3 genes SIM1 and MRAP2 in Prader Willi like patients.
Molecular genetics and metabolism117(3)383-8(2016 Mar)
Geets E,Zegers D,Beckers S,Verrijken A,Massa G,Van Hoorenbeeck K,Verhulst S,Van Gaal L,Van Hul W

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SIM1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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