gRNAs
The following SIM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OBa01724 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001192972.3 , NM_001192972.1 | |
| Accession Version | NM_001192972.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-29 | |
| Organism | Bos taurus(cattle) | |
| Product | single-minded homolog 1 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000009.1. On May 30, 2018 this sequence version replaced NM_001192972.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145420, SAMN04019645 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGAAAGAGA AGTCCAAAAA TGCTGCCCGG ACTAGGAGGG AGAAGGAAAA CAGTGAATTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001179901.3 |
| CDS | 1..2301 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001192972.3 |
1 | ATGAAAGAGA AGTCCAAAAA TGCTGCCCGG ACTAGGAGGG AGAAGGAAAA CAGTGAATTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa01724 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001192972.3 , NM_001192972.1 | |
| Accession Version | NM_001192972.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2011-10-24 | |
| Organism | Bos taurus(Cattle) | |
| Product | single-minded homolog 1 | |
| Comment | PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03114033.1, AAFC03088356.1, AAFC03088357.1 and AAFC03114031.1. On Jul 17, 2010 this sequence version replaced XM_864611.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. | |
1 | ATGAAAGAGA AGTCCAAAAA TGCTGCCCGG ACTAGGAGGG AGAAGGAAAA CAGTGAATTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001179901.1 |
| CDS | 1..2301 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001192972.1 |
1 | ATGAAAGAGA AGTCCAAAAA TGCTGCCCGG ACTAGGAGGG AGAAGGAAAA CAGTGAATTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa91469 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001192972.2 , XM_015472829.1 | |
| Accession Version | NM_001192972.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-10-22 | |
| Organism | Bos taurus(cattle) | |
| Product | single-minded homolog 1 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02026200.1 and DAAA02026201.1. On Jun 30, 2017 this sequence version replaced NM_001192972.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822095, SAMN04019645 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGAAAGAGA AGTCCAAAAA TGCTGCCCGG ACTAGGAGGG AGAAGGAAAA CAGTGAATTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001179901.2 |
| CDS | 1..2301 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001192972.2 |
1 | ATGAAAGAGA AGTCCAAAAA TGCTGCCCGG ACTAGGAGGG AGAAGGAAAA CAGTGAATTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa91469 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001192972.2 , XM_015472829.1 | |
| Accession Version | XM_015472829.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-25 | |
| Organism | Bos taurus(cattle) | |
| Product | single-minded homolog 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104201.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAAAGAGA AGTCCAAAAA TGCTGCCCGG ACTAGGAGGG AGAAGGAAAA CAGTGAATTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015328315.1 |
| CDS | 752..3052 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015472829.1 |
1 | ATGAAAGAGA AGTCCAAAAA TGCTGCCCGG ACTAGGAGGG AGAAGGAAAA CAGTGAATTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
A whole-genome assembly of the domestic cow, Bos taurus. |
