dhx15 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	dhx15
Entrez Gene ID	100216120
Full Name	DEAH-box helicase 15
General protein information	Preferred Names DEAH-box helicase 15 Names pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 DEAH (Asp-Glu-Ala-His) box helicase 15 DEAH (Asp-Glu-Ala-His) box polypeptide 15
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012957451.2	XP_012812905.1	pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 isoform X1
NM_001142099.1	NP_001135571.1	pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15
XM_018091366.1	XP_017946855.1	pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 isoform X2
XM_012957451.3	XP_012812905.1	pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Dhx15	NP_031865.2
Saccharomyces cerevisiae (baker's yeast)	PRP43	NP_011395.1
Schizosaccharomyces pombe (fission yeast)	prp43	NP_595937.1
Oryza sativa (rice)	Os03g0314100	NP_001049931.1
Rattus norvegicus (Norway rat)	Dhx15	NP_001178526.1
Gallus gallus (chicken)	DHX15	NP_001026330.1
Danio rerio (zebrafish)	dhx15	NP_001108613.1
Kluyveromyces lactis	KLLA0B00561g	XP_451555.1
Magnaporthe oryzae (rice blast fungus)	MGG_03893	XP_003719983.1
Xenopus tropicalis (tropical clawed frog)	dhx15	NP_001135571.1
	DHX15	XP_001106067.1
Bos taurus (cattle)	DHX15	NP_001091487.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011149	XP_309498.4
Caenorhabditis elegans (roundworm)	F56D2.6	NP_741147.1
Homo sapiens (human)	DHX15	NP_001349.2
Drosophila melanogaster (fruit fly)	CG11107	NP_610269.1
Arabidopsis thaliana (thale cress)	AT3G62310	NP_191790.1
Pan troglodytes (chimpanzee)	DHX15	XP_001164599.1
Canis lupus familiaris (dog)	DHX15	XP_851535.1
Eremothecium gossypii	AGOS_AAR180C	NP_982723.1
Neurospora crassa	NCU01612	XP_956523.2
Arabidopsis thaliana (thale cress)	AT2G47250	NP_182247.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dhx15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dhx15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa21234	NM_001142099.1 Latest version!	Xenopus tropicalis DEAH-box helicase 15 (dhx15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa32605	XM_012957451.3 Latest version!	Xenopus tropicalis DEAH-box helicase 15 (dhx15), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa41716	XM_018091366.1 Latest version!	Xenopus tropicalis DEAH-box helicase 15 (dhx15), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OXa32605	XM_012957451.2	Xenopus tropicalis DEAH-box helicase 15 (dhx15), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dhx15 ( NM_001142099.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001135571.1 Xenopus tropicalis DEAH-box helicase 15 (dhx15), mRNA.

Price & Availability↑

CloneID	OXa21234
Clone ID Related Accession (Same CDS sequence)	NM_001142099.1
Accession Version	NM_001142099.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC168075.1. ##Evidence-Data-START## CDS exon combination :: BC168075.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGTCGAAGC GGCACCGGCT GGACCTAGGG GACGACTATG TGGCGAACAA GAAAAGAAGC 
AGCGACGGAA AAGACAGAGA TCGAGAGAGA GATGACCGAT CTAAGGACCG GGACCGTGAC 
AGAGATAGGG ATCGGGAAAG GGACAGAGAC AAGGATAAAG ACTCACGTGG GTCATCCAAT 
TCTACGTATA TTACAGCTAG TGGAATGCCA CCTCTAAAAC CTGCAGCGGT TGCTCAAGGC 
ATTAATCCCT TTACCAACTT ACCCCACACT CCGCGCTACT ATGACATCTT AAAAAAGAGG 
CTACTGTTGC CAGTATGGGA ATACAAAGAA CGGTTTACTG AAATTCTTAT GAAGAACCAG 
AGCTTTGTAT TGGTTGGTGA GACTGGATCT GGGAAAACTA CTCAGATTCC ACAGTGGTGT 
GTCGACTACA TGAGGTTATT GCCTGGTCCC AAAAGAGGCG TTGCATGTAC CCAGCCAAGA 
AGAGTGGCTG CCATGAGTGT GGCTCAAAGA GTTGCAGATG AAATGGATGT CATGTTGGGT 
CAGGAAGTTG GTTATTCCAT TAGGTTTGAA GACTGCAGCA GTGCAAAAAC AATCTTGAAG 
TACATGACAG ACGGTATGTT ACTCCGGGAA GCTATGAACG ATCCATTGTT GGAACGCTAT 
GGGGTAGTGA TTCTTGACGA AGCTCATGAA AGGACCTTAG CAACAGATAT TCTAATGGGT 
GTTTTAAAGG AAGTTGTAAG ACAAAGGAAT GACTTAAAGG TTATAGTTAT GAGTGCCACT 
TTAGATGCTG GCAAATTCCA GGTATATTTT GATAGCTGCC CTCTTTTAAC AATCCCTGGA 
CGAACACACC CTGTAGAAAT CTTCTATACT CCAGAGCCTG AGCGGGATTA CCTAGAGGCA 
GCCATTCGAA CAGTGATTCA GATTCACATG TGTGAAGAGG AGGAAGGAGA TCTCCTGCTT 
TTTCTTACTG GACAAGAGGA AATTGACGAG GCCTGCAAGA GAATCAAACG GGAAATCGAT 
GACTTGGGCC CTGATGTTGG AGACATCAAA ATCATTCCTT TATATTCAAC CCTCCCACCT 
CAGCAACAGC AAAGAATATT TGAGCCTCCT CCACCCAAGA AACCTAGTGG AGCAATAGGA 
AGGAAAGTTG TAGTTTCCAC TAACATCGCT GAAACATCTC TGACTATAGA TGGTGTTGTG 
TTTGTGATCG ATCCTGGCTT TGCCAAGCAA AAGGTGTATA ACCCTCGAAT TCGAGTGGAA 
TCTCTCCTGG TGACTGCCAT CAGCAAAGCT TCAGCGCAGC AAAGAGCTGG TCGTGCAGGT 
CGTACCCGGC CTGGCAAGTG CTTCCGGCTT TATACAGAAA AGGCTTACAA AACAGAAATG 
CAGGACAATA CTTACCCTGA AATATTGAGG TCCAATTTGG GTTCCGTAGT ATTGCAGTTA 
AAGAAACTTG GCATTGATGA CTTGGTTCAT TTTGATTTCA TGGACCCTCC AGCGCCTGAA 
ACTTTAATGC GTGCCCTGGA GCTTTTGAAC TACCTGGCTG CCTTAAATGA CGACGGAGAC 
CTGACAGAAT TGGGATCTAT GATGGCAGAG TTCCCACTGG ACCCACAGCT GGCCAAGATG 
GTTATTGCCA GTTGTGACTA CAACTGCTCT AATGAGATCT TATCCATAAC AGCCATGTTG 
TCAGTCCCAC AGTGTTTCAT TCGACCCACG GAAGTCAAGA AAGCTGCCGA TGAGGCTAAG 
ATGAGGTTTG CCCACATCGA CGGTGATCAT TTGACGCTGC TGAATGTATA CCATGCTTTC 
AAGCAGAATC ACGAGTCGTC TCAGTGGTGT TATGACAACT TCATTAACTA CAGATCCCTG 
ATGTCTGCAG ACAATGTACG GCAGCAGCTG TCACGAATCA TGGACAGATT TAACTTGCCG 
CGAAGAAGCA CAGACTTTAC AAGCCGAGAT TATTACATTA ATATTAGGAA AGCTTTGGTT 
ACTGGTTATT TTATGCAGGT GGCACACTTG GAGCGAACTG GTCACTATCT AACAGTGAAA 
GACAACCAAG TGGTACAGTT GCACCCGTCA ACCGTTCTTG ACCACAAACC CGAGTGGGTC 
CTGTACAACG AGTTTGTCCT CACAACCAAG AACTACATCC GCACATGCAC AGATATAAAG 
CCTGAATGGT TGGTGAAAAT TGCAGCTCAA TATTACGACA TGAGCAACTT TCCTCAGTGT 
GAAGCAAAGA GGCAACTGGA GCGCATTGTG GCCAAACTTC AGACTAAGGA ATATTCACAG 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135571.1
CDS	65..2350
Translation	MSKRHRLDLGDDYVANKKRSSDGKDRDRERDDRSKDRDRDRDRDRERDRDKDKDSRGSSNSTYITASGMPPLKPAAVAQGINPFTNLPHTPRYYDILKKRLLLPVWEYKERFTEILMKNQSFVLVGETGSGKTTQIPQWCVDYMRLLPGPKRGVACTQPRRVAAMSVAQRVADEMDVMLGQEVGYSIRFEDCSSAKTILKYMTDGMLLREAMNDPLLERYGVVILDEAHERTLATDILMGVLKEVVRQRNDLKVIVMSATLDAGKFQVYFDSCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHMCEEEEGDLLLFLTGQEEIDEACKRIKREIDDLGPDVGDIKIIPLYSTLPPQQQQRIFEPPPPKKPSGAIGRKVVVSTNIAETSLTIDGVVFVIDPGFAKQKVYNPRIRVESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAYKTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALNDDGDLTELGSMMAEFPLDPQLAKMVIASCDYNCSNEILSITAMLSVPQCFIRPTEVKKAADEAKMRFAHIDGDHLTLLNVYHAFKQNHESSQWCYDNFINYRSLMSADNVRQQLSRIMDRFNLPRRSTDFTSRDYYINIRKALVTGYFMQVAHLERTGHYLTVKDNQVVQLHPSTVLDHKPEWVLYNEFVLTTKNYIRTCTDIKPEWLVKIAAQYYDMSNFPQCEAKRQLERIVAKLQTKEYSQY

Target ORF information:

RefSeq Version	NM_001142099.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAH-box helicase 15 (dhx15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142099.1

ORF Insert Sequence:

ATGTCGAAGC GGCACCGGCT GGACCTAGGG GACGACTATG TGGCGAACAA GAAAAGAAGC 
AGCGACGGAA AAGACAGAGA TCGAGAGAGA GATGACCGAT CTAAGGACCG GGACCGTGAC 
AGAGATAGGG ATCGGGAAAG GGACAGAGAC AAGGATAAAG ACTCACGTGG GTCATCCAAT 
TCTACGTATA TTACAGCTAG TGGAATGCCA CCTCTAAAAC CTGCAGCGGT TGCTCAAGGC 
ATTAATCCCT TTACCAACTT ACCCCACACT CCGCGCTACT ATGACATCTT AAAAAAGAGG 
CTACTGTTGC CAGTATGGGA ATACAAAGAA CGGTTTACTG AAATTCTTAT GAAGAACCAG 
AGCTTTGTAT TGGTTGGTGA GACTGGATCT GGGAAAACTA CTCAGATTCC ACAGTGGTGT 
GTCGACTACA TGAGGTTATT GCCTGGTCCC AAAAGAGGCG TTGCATGTAC CCAGCCAAGA 
AGAGTGGCTG CCATGAGTGT GGCTCAAAGA GTTGCAGATG AAATGGATGT CATGTTGGGT 
CAGGAAGTTG GTTATTCCAT TAGGTTTGAA GACTGCAGCA GTGCAAAAAC AATCTTGAAG 
TACATGACAG ACGGTATGTT ACTCCGGGAA GCTATGAACG ATCCATTGTT GGAACGCTAT 
GGGGTAGTGA TTCTTGACGA AGCTCATGAA AGGACCTTAG CAACAGATAT TCTAATGGGT 
GTTTTAAAGG AAGTTGTAAG ACAAAGGAAT GACTTAAAGG TTATAGTTAT GAGTGCCACT 
TTAGATGCTG GCAAATTCCA GGTATATTTT GATAGCTGCC CTCTTTTAAC AATCCCTGGA 
CGAACACACC CTGTAGAAAT CTTCTATACT CCAGAGCCTG AGCGGGATTA CCTAGAGGCA 
GCCATTCGAA CAGTGATTCA GATTCACATG TGTGAAGAGG AGGAAGGAGA TCTCCTGCTT 
TTTCTTACTG GACAAGAGGA AATTGACGAG GCCTGCAAGA GAATCAAACG GGAAATCGAT 
GACTTGGGCC CTGATGTTGG AGACATCAAA ATCATTCCTT TATATTCAAC CCTCCCACCT 
CAGCAACAGC AAAGAATATT TGAGCCTCCT CCACCCAAGA AACCTAGTGG AGCAATAGGA 
AGGAAAGTTG TAGTTTCCAC TAACATCGCT GAAACATCTC TGACTATAGA TGGTGTTGTG 
TTTGTGATCG ATCCTGGCTT TGCCAAGCAA AAGGTGTATA ACCCTCGAAT TCGAGTGGAA 
TCTCTCCTGG TGACTGCCAT CAGCAAAGCT TCAGCGCAGC AAAGAGCTGG TCGTGCAGGT 
CGTACCCGGC CTGGCAAGTG CTTCCGGCTT TATACAGAAA AGGCTTACAA AACAGAAATG 
CAGGACAATA CTTACCCTGA AATATTGAGG TCCAATTTGG GTTCCGTAGT ATTGCAGTTA 
AAGAAACTTG GCATTGATGA CTTGGTTCAT TTTGATTTCA TGGACCCTCC AGCGCCTGAA 
ACTTTAATGC GTGCCCTGGA GCTTTTGAAC TACCTGGCTG CCTTAAATGA CGACGGAGAC 
CTGACAGAAT TGGGATCTAT GATGGCAGAG TTCCCACTGG ACCCACAGCT GGCCAAGATG 
GTTATTGCCA GTTGTGACTA CAACTGCTCT AATGAGATCT TATCCATAAC AGCCATGTTG 
TCAGTCCCAC AGTGTTTCAT TCGACCCACG GAAGTCAAGA AAGCTGCCGA TGAGGCTAAG 
ATGAGGTTTG CCCACATCGA CGGTGATCAT TTGACGCTGC TGAATGTATA CCATGCTTTC 
AAGCAGAATC ACGAGTCGTC TCAGTGGTGT TATGACAACT TCATTAACTA CAGATCCCTG 
ATGTCTGCAG ACAATGTACG GCAGCAGCTG TCACGAATCA TGGACAGATT TAACTTGCCG 
CGAAGAAGCA CAGACTTTAC AAGCCGAGAT TATTACATTA ATATTAGGAA AGCTTTGGTT 
ACTGGTTATT TTATGCAGGT GGCACACTTG GAGCGAACTG GTCACTATCT AACAGTGAAA 
GACAACCAAG TGGTACAGTT GCACCCGTCA ACCGTTCTTG ACCACAAACC CGAGTGGGTC 
CTGTACAACG AGTTTGTCCT CACAACCAAG AACTACATCC GCACATGCAC AGATATAAAG 
CCTGAATGGT TGGTGAAAAT TGCAGCTCAA TATTACGACA TGAGCAACTT TCCTCAGTGT 
GAAGCAAAGA GGCAACTGGA GCGCATTGTG GCCAAACTTC AGACTAAGGA ATATTCACAG 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dhx15 ( XM_012957451.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812905.1 Xenopus tropicalis DEAH-box helicase 15 (dhx15), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa32605
Clone ID Related Accession (Same CDS sequence)	XM_012957451.2 , XM_012957451.3
Accession Version	XM_012957451.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957451.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCCC CTCTCCAAAG AAAAGACAGA GATCGAGAGA GAGATGACCG ATCTAAGGAC 
CGGGACCGTG ACAGAGATAG GGATCGGGAA AGGGACAGAG ACAAGGATAA AGACTCACGT 
GGGTCATCCA ATTCTACGTA TATTACAGCT AGTGGAATGC CACCTCTAAA ACCTGCAGCG 
GTTGCTCAAG GCATTAATCC CTTTACCAAC TTACCCCACA CTCCGCGCTA CTATGACATC 
TTAAAAAAGA GGCTACTGTT GCCAGTATGG GAATACAAAG AACGGTTTAC TGAAATTCTT 
ATGAAGAACC AGAGCTTTGT ATTGGTTGGT GAGACTGGAT CTGGGAAAAC TACTCAGATT 
CCACAGTGGT GTGTCGACTA CATGAGGTCA TTGCCTGGTC CCAAAAGAGG CGTTGCATGT 
ACCCAGCCAA GAAGAGTGGC TGCCATGAGT GTGGCTCAAA GAGTTGCAGA TGAAATGGAT 
GTCATGTTGG GTCAGGAAGT TGGTTATTCC ATTAGGTTTG AAGACTGCAG CAGTGCAAAA 
ACAATCTTGA AGTACATGAC AGACGGTATG TTACTCCGGG AAGCTATGAA CGATCCATTG 
TTGGAACGCT ATGGGGTAGT GATTCTTGAC GAAGCTCATG AAAGGACCTT AGCAACAGAT 
ATTCTAATGG GTGTTTTAAA GGAAGTTGTA AGACAAAGGA ATGACTTAAA GGTTATAGTT 
ATGAGTGCCA CTTTAGATGC TGGCAAATTC CAGGTATATT TTGATAGCTG CCCTCTTTTA 
ACAATCCCTG GACGAACACA CCCTGTAGAA ATCTTCTATA CTCCAGAGCC TGAGCGGGAT 
TACCTAGAGG CAGCCATTCG AACAGTGATT CAGATTCACA TGTGTGAAGA GGAGGAAGGA 
GATCTCCTGC TTTTTCTTAC TGGACAAGAG GAAATTGACG AGGCCTGCAA GAGAATCAAA 
CGGGAAATCG ATGACTTGGG CCCTGATGTA GGAGACATCA AAATCATTCC TTTATATTCA 
ACCCTCCCAC CTCAGCAACA GCAAAGAATA TTTGAGCCTC CTCCACCCAA GAAACCTAGT 
GGAGCAATAG GAAGGAAAGT TGTAGTTTCC ACTAACATCG CTGAAACATC TCTGACTATA 
GATGGTGTTG TGTTTGTGAT CGATCCTGGC TTTGCCAAGC AAAAGGTGTA TAACCCTCGA 
ATTCGAGTGG AATCTCTCCT GGTGACTGCC ATCAGCAAAG CTTCAGCGCA GCAAAGAGCT 
GGTCGTGCAG GTCGTACCCG GCCTGGCAAG TGCTTCCGGC TTTATACAGA AAAGGCTTAC 
AAAACAGAAA TGCAGGACAA TACTTACCCT GAAATATTGA GGTCCAATTT GGGTTCCGTA 
GTATTGCAGT TAAAGAAACT TGGCATTGAT GACTTGGTTC ATTTTGATTT CATGGACCCT 
CCAGCGCCTG AAACTTTAAT GCGTGCCCTG GAGCTTTTGA ACTACCTGGC TGCCTTAAAT 
GACGACGGAG ACCTGACAGA ATTGGGATCT ATGATGGCAG AGTTCCCACT GGACCCACAG 
CTGGCCAAGA TGGTTATTGC CAGTTGTGAC TACAACTGCT CTAATGAGAT CTTATCCATA 
ACAGCCATGT TGTCAGTCCC ACAGTGTTTC ATTCGACCCA CGGAAGTCAA GAAAGCTGCC 
GATGAGGCTA AGATGAGGTT TGCCCACATC GACGGTGATC ATTTGACGCT GCTGAATGTA 
TACCATGCTT TCAAGCAGAA TCACGAGTCG TCTCAGTGGT GTTATGACAA CTTCATTAAC 
TACAGATCCC TGATGTCTGC AGACAATGTA CGGCAGCAGC TGTCACGAAT CATGGACAGA 
TTTAACTTGC CGCGAAGAAG CACAGACTTT ACAAGCCGAG ATTATTACAT TAATATTAGG 
AAAGCTTTGG TTACTGGTTA TTTTATGCAG GTGGCACACT TGGAGCGAAC TGGTCACTAT 
CTAACAGTGA AAGACAACCA AGTGGTACAG TTGCACCCGT CAACCGTTCT TGACCACAAA 
CCCGAGTGGG TCCTGTACAA CGAGTTTGTC CTCACAACCA AGAACTACAT CCGCACATGC 
ACAGATATAA AGCCTGAATG GTTGGTGAAA ATTGCAGCTC AATATTACGA CATGAGCAAC 
TTTCCTCAGT GTGAAGCAAA GAGGCAACTG GAGCGCATTG TGGCCAAACT TCAGACTAAG 
GAATATTCAC AGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812905.1
CDS	83..2320
Translation	MGPPLQRKDRDRERDDRSKDRDRDRDRDRERDRDKDKDSRGSSNSTYITASGMPPLKPAAVAQGINPFTNLPHTPRYYDILKKRLLLPVWEYKERFTEILMKNQSFVLVGETGSGKTTQIPQWCVDYMRSLPGPKRGVACTQPRRVAAMSVAQRVADEMDVMLGQEVGYSIRFEDCSSAKTILKYMTDGMLLREAMNDPLLERYGVVILDEAHERTLATDILMGVLKEVVRQRNDLKVIVMSATLDAGKFQVYFDSCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHMCEEEEGDLLLFLTGQEEIDEACKRIKREIDDLGPDVGDIKIIPLYSTLPPQQQQRIFEPPPPKKPSGAIGRKVVVSTNIAETSLTIDGVVFVIDPGFAKQKVYNPRIRVESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAYKTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALNDDGDLTELGSMMAEFPLDPQLAKMVIASCDYNCSNEILSITAMLSVPQCFIRPTEVKKAADEAKMRFAHIDGDHLTLLNVYHAFKQNHESSQWCYDNFINYRSLMSADNVRQQLSRIMDRFNLPRRSTDFTSRDYYINIRKALVTGYFMQVAHLERTGHYLTVKDNQVVQLHPSTVLDHKPEWVLYNEFVLTTKNYIRTCTDIKPEWLVKIAAQYYDMSNFPQCEAKRQLERIVAKLQTKEYSQY

Target ORF information:

RefSeq Version	XM_012957451.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAH-box helicase 15 (dhx15), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957451.2

ORF Insert Sequence:

ATGGGGCCCC CTCTCCAAAG AAAAGACAGA GATCGAGAGA GAGATGACCG ATCTAAGGAC 
CGGGACCGTG ACAGAGATAG GGATCGGGAA AGGGACAGAG ACAAGGATAA AGACTCACGT 
GGGTCATCCA ATTCTACGTA TATTACAGCT AGTGGAATGC CACCTCTAAA ACCTGCAGCG 
GTTGCTCAAG GCATTAATCC CTTTACCAAC TTACCCCACA CTCCGCGCTA CTATGACATC 
TTAAAAAAGA GGCTACTGTT GCCAGTATGG GAATACAAAG AACGGTTTAC TGAAATTCTT 
ATGAAGAACC AGAGCTTTGT ATTGGTTGGT GAGACTGGAT CTGGGAAAAC TACTCAGATT 
CCACAGTGGT GTGTCGACTA CATGAGGTCA TTGCCTGGTC CCAAAAGAGG CGTTGCATGT 
ACCCAGCCAA GAAGAGTGGC TGCCATGAGT GTGGCTCAAA GAGTTGCAGA TGAAATGGAT 
GTCATGTTGG GTCAGGAAGT TGGTTATTCC ATTAGGTTTG AAGACTGCAG CAGTGCAAAA 
ACAATCTTGA AGTACATGAC AGACGGTATG TTACTCCGGG AAGCTATGAA CGATCCATTG 
TTGGAACGCT ATGGGGTAGT GATTCTTGAC GAAGCTCATG AAAGGACCTT AGCAACAGAT 
ATTCTAATGG GTGTTTTAAA GGAAGTTGTA AGACAAAGGA ATGACTTAAA GGTTATAGTT 
ATGAGTGCCA CTTTAGATGC TGGCAAATTC CAGGTATATT TTGATAGCTG CCCTCTTTTA 
ACAATCCCTG GACGAACACA CCCTGTAGAA ATCTTCTATA CTCCAGAGCC TGAGCGGGAT 
TACCTAGAGG CAGCCATTCG AACAGTGATT CAGATTCACA TGTGTGAAGA GGAGGAAGGA 
GATCTCCTGC TTTTTCTTAC TGGACAAGAG GAAATTGACG AGGCCTGCAA GAGAATCAAA 
CGGGAAATCG ATGACTTGGG CCCTGATGTA GGAGACATCA AAATCATTCC TTTATATTCA 
ACCCTCCCAC CTCAGCAACA GCAAAGAATA TTTGAGCCTC CTCCACCCAA GAAACCTAGT 
GGAGCAATAG GAAGGAAAGT TGTAGTTTCC ACTAACATCG CTGAAACATC TCTGACTATA 
GATGGTGTTG TGTTTGTGAT CGATCCTGGC TTTGCCAAGC AAAAGGTGTA TAACCCTCGA 
ATTCGAGTGG AATCTCTCCT GGTGACTGCC ATCAGCAAAG CTTCAGCGCA GCAAAGAGCT 
GGTCGTGCAG GTCGTACCCG GCCTGGCAAG TGCTTCCGGC TTTATACAGA AAAGGCTTAC 
AAAACAGAAA TGCAGGACAA TACTTACCCT GAAATATTGA GGTCCAATTT GGGTTCCGTA 
GTATTGCAGT TAAAGAAACT TGGCATTGAT GACTTGGTTC ATTTTGATTT CATGGACCCT 
CCAGCGCCTG AAACTTTAAT GCGTGCCCTG GAGCTTTTGA ACTACCTGGC TGCCTTAAAT 
GACGACGGAG ACCTGACAGA ATTGGGATCT ATGATGGCAG AGTTCCCACT GGACCCACAG 
CTGGCCAAGA TGGTTATTGC CAGTTGTGAC TACAACTGCT CTAATGAGAT CTTATCCATA 
ACAGCCATGT TGTCAGTCCC ACAGTGTTTC ATTCGACCCA CGGAAGTCAA GAAAGCTGCC 
GATGAGGCTA AGATGAGGTT TGCCCACATC GACGGTGATC ATTTGACGCT GCTGAATGTA 
TACCATGCTT TCAAGCAGAA TCACGAGTCG TCTCAGTGGT GTTATGACAA CTTCATTAAC 
TACAGATCCC TGATGTCTGC AGACAATGTA CGGCAGCAGC TGTCACGAAT CATGGACAGA 
TTTAACTTGC CGCGAAGAAG CACAGACTTT ACAAGCCGAG ATTATTACAT TAATATTAGG 
AAAGCTTTGG TTACTGGTTA TTTTATGCAG GTGGCACACT TGGAGCGAAC TGGTCACTAT 
CTAACAGTGA AAGACAACCA AGTGGTACAG TTGCACCCGT CAACCGTTCT TGACCACAAA 
CCCGAGTGGG TCCTGTACAA CGAGTTTGTC CTCACAACCA AGAACTACAT CCGCACATGC 
ACAGATATAA AGCCTGAATG GTTGGTGAAA ATTGCAGCTC AATATTACGA CATGAGCAAC 
TTTCCTCAGT GTGAAGCAAA GAGGCAACTG GAGCGCATTG TGGCCAAACT TCAGACTAAG 
GAATATTCAC AGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dhx15 ( XM_012957451.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812905.1 Xenopus tropicalis DEAH-box helicase 15 (dhx15), transcript variant X1, mRNA.

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CloneID	OXa32605
Clone ID Related Accession (Same CDS sequence)	XM_012957451.2 , XM_012957451.3
Accession Version	XM_012957451.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957451.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCCC CTCTCCAAAG AAAAGACAGA GATCGAGAGA GAGATGACCG ATCTAAGGAC 
CGGGACCGTG ACAGAGATAG GGATCGGGAA AGGGACAGAG ACAAGGATAA AGACTCACGT 
GGGTCATCCA ATTCTACGTA TATTACAGCT AGTGGAATGC CACCTCTAAA ACCTGCAGCG 
GTTGCTCAAG GCATTAATCC CTTTACCAAC TTACCCCACA CTCCGCGCTA CTATGACATC 
TTAAAAAAGA GGCTACTGTT GCCAGTATGG GAATACAAAG AACGGTTTAC TGAAATTCTT 
ATGAAGAACC AGAGCTTTGT ATTGGTTGGT GAGACTGGAT CTGGGAAAAC TACTCAGATT 
CCACAGTGGT GTGTCGACTA CATGAGGTCA TTGCCTGGTC CCAAAAGAGG CGTTGCATGT 
ACCCAGCCAA GAAGAGTGGC TGCCATGAGT GTGGCTCAAA GAGTTGCAGA TGAAATGGAT 
GTCATGTTGG GTCAGGAAGT TGGTTATTCC ATTAGGTTTG AAGACTGCAG CAGTGCAAAA 
ACAATCTTGA AGTACATGAC AGACGGTATG TTACTCCGGG AAGCTATGAA CGATCCATTG 
TTGGAACGCT ATGGGGTAGT GATTCTTGAC GAAGCTCATG AAAGGACCTT AGCAACAGAT 
ATTCTAATGG GTGTTTTAAA GGAAGTTGTA AGACAAAGGA ATGACTTAAA GGTTATAGTT 
ATGAGTGCCA CTTTAGATGC TGGCAAATTC CAGGTATATT TTGATAGCTG CCCTCTTTTA 
ACAATCCCTG GACGAACACA CCCTGTAGAA ATCTTCTATA CTCCAGAGCC TGAGCGGGAT 
TACCTAGAGG CAGCCATTCG AACAGTGATT CAGATTCACA TGTGTGAAGA GGAGGAAGGA 
GATCTCCTGC TTTTTCTTAC TGGACAAGAG GAAATTGACG AGGCCTGCAA GAGAATCAAA 
CGGGAAATCG ATGACTTGGG CCCTGATGTA GGAGACATCA AAATCATTCC TTTATATTCA 
ACCCTCCCAC CTCAGCAACA GCAAAGAATA TTTGAGCCTC CTCCACCCAA GAAACCTAGT 
GGAGCAATAG GAAGGAAAGT TGTAGTTTCC ACTAACATCG CTGAAACATC TCTGACTATA 
GATGGTGTTG TGTTTGTGAT CGATCCTGGC TTTGCCAAGC AAAAGGTGTA TAACCCTCGA 
ATTCGAGTGG AATCTCTCCT GGTGACTGCC ATCAGCAAAG CTTCAGCGCA GCAAAGAGCT 
GGTCGTGCAG GTCGTACCCG GCCTGGCAAG TGCTTCCGGC TTTATACAGA AAAGGCTTAC 
AAAACAGAAA TGCAGGACAA TACTTACCCT GAAATATTGA GGTCCAATTT GGGTTCCGTA 
GTATTGCAGT TAAAGAAACT TGGCATTGAT GACTTGGTTC ATTTTGATTT CATGGACCCT 
CCAGCGCCTG AAACTTTAAT GCGTGCCCTG GAGCTTTTGA ACTACCTGGC TGCCTTAAAT 
GACGACGGAG ACCTGACAGA ATTGGGATCT ATGATGGCAG AGTTCCCACT GGACCCACAG 
CTGGCCAAGA TGGTTATTGC CAGTTGTGAC TACAACTGCT CTAATGAGAT CTTATCCATA 
ACAGCCATGT TGTCAGTCCC ACAGTGTTTC ATTCGACCCA CGGAAGTCAA GAAAGCTGCC 
GATGAGGCTA AGATGAGGTT TGCCCACATC GACGGTGATC ATTTGACGCT GCTGAATGTA 
TACCATGCTT TCAAGCAGAA TCACGAGTCG TCTCAGTGGT GTTATGACAA CTTCATTAAC 
TACAGATCCC TGATGTCTGC AGACAATGTA CGGCAGCAGC TGTCACGAAT CATGGACAGA 
TTTAACTTGC CGCGAAGAAG CACAGACTTT ACAAGCCGAG ATTATTACAT TAATATTAGG 
AAAGCTTTGG TTACTGGTTA TTTTATGCAG GTGGCACACT TGGAGCGAAC TGGTCACTAT 
CTAACAGTGA AAGACAACCA AGTGGTACAG TTGCACCCGT CAACCGTTCT TGACCACAAA 
CCCGAGTGGG TCCTGTACAA CGAGTTTGTC CTCACAACCA AGAACTACAT CCGCACATGC 
ACAGATATAA AGCCTGAATG GTTGGTGAAA ATTGCAGCTC AATATTACGA CATGAGCAAC 
TTTCCTCAGT GTGAAGCAAA GAGGCAACTG GAGCGCATTG TGGCCAAACT TCAGACTAAG 
GAATATTCAC AGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812905.1
CDS	72..2309
Translation	MGPPLQRKDRDRERDDRSKDRDRDRDRDRERDRDKDKDSRGSSNSTYITASGMPPLKPAAVAQGINPFTNLPHTPRYYDILKKRLLLPVWEYKERFTEILMKNQSFVLVGETGSGKTTQIPQWCVDYMRSLPGPKRGVACTQPRRVAAMSVAQRVADEMDVMLGQEVGYSIRFEDCSSAKTILKYMTDGMLLREAMNDPLLERYGVVILDEAHERTLATDILMGVLKEVVRQRNDLKVIVMSATLDAGKFQVYFDSCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHMCEEEEGDLLLFLTGQEEIDEACKRIKREIDDLGPDVGDIKIIPLYSTLPPQQQQRIFEPPPPKKPSGAIGRKVVVSTNIAETSLTIDGVVFVIDPGFAKQKVYNPRIRVESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAYKTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALNDDGDLTELGSMMAEFPLDPQLAKMVIASCDYNCSNEILSITAMLSVPQCFIRPTEVKKAADEAKMRFAHIDGDHLTLLNVYHAFKQNHESSQWCYDNFINYRSLMSADNVRQQLSRIMDRFNLPRRSTDFTSRDYYINIRKALVTGYFMQVAHLERTGHYLTVKDNQVVQLHPSTVLDHKPEWVLYNEFVLTTKNYIRTCTDIKPEWLVKIAAQYYDMSNFPQCEAKRQLERIVAKLQTKEYSQY

Target ORF information:

RefSeq Version	XM_012957451.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAH-box helicase 15 (dhx15), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957451.3

ORF Insert Sequence:

ATGGGGCCCC CTCTCCAAAG AAAAGACAGA GATCGAGAGA GAGATGACCG ATCTAAGGAC 
CGGGACCGTG ACAGAGATAG GGATCGGGAA AGGGACAGAG ACAAGGATAA AGACTCACGT 
GGGTCATCCA ATTCTACGTA TATTACAGCT AGTGGAATGC CACCTCTAAA ACCTGCAGCG 
GTTGCTCAAG GCATTAATCC CTTTACCAAC TTACCCCACA CTCCGCGCTA CTATGACATC 
TTAAAAAAGA GGCTACTGTT GCCAGTATGG GAATACAAAG AACGGTTTAC TGAAATTCTT 
ATGAAGAACC AGAGCTTTGT ATTGGTTGGT GAGACTGGAT CTGGGAAAAC TACTCAGATT 
CCACAGTGGT GTGTCGACTA CATGAGGTCA TTGCCTGGTC CCAAAAGAGG CGTTGCATGT 
ACCCAGCCAA GAAGAGTGGC TGCCATGAGT GTGGCTCAAA GAGTTGCAGA TGAAATGGAT 
GTCATGTTGG GTCAGGAAGT TGGTTATTCC ATTAGGTTTG AAGACTGCAG CAGTGCAAAA 
ACAATCTTGA AGTACATGAC AGACGGTATG TTACTCCGGG AAGCTATGAA CGATCCATTG 
TTGGAACGCT ATGGGGTAGT GATTCTTGAC GAAGCTCATG AAAGGACCTT AGCAACAGAT 
ATTCTAATGG GTGTTTTAAA GGAAGTTGTA AGACAAAGGA ATGACTTAAA GGTTATAGTT 
ATGAGTGCCA CTTTAGATGC TGGCAAATTC CAGGTATATT TTGATAGCTG CCCTCTTTTA 
ACAATCCCTG GACGAACACA CCCTGTAGAA ATCTTCTATA CTCCAGAGCC TGAGCGGGAT 
TACCTAGAGG CAGCCATTCG AACAGTGATT CAGATTCACA TGTGTGAAGA GGAGGAAGGA 
GATCTCCTGC TTTTTCTTAC TGGACAAGAG GAAATTGACG AGGCCTGCAA GAGAATCAAA 
CGGGAAATCG ATGACTTGGG CCCTGATGTA GGAGACATCA AAATCATTCC TTTATATTCA 
ACCCTCCCAC CTCAGCAACA GCAAAGAATA TTTGAGCCTC CTCCACCCAA GAAACCTAGT 
GGAGCAATAG GAAGGAAAGT TGTAGTTTCC ACTAACATCG CTGAAACATC TCTGACTATA 
GATGGTGTTG TGTTTGTGAT CGATCCTGGC TTTGCCAAGC AAAAGGTGTA TAACCCTCGA 
ATTCGAGTGG AATCTCTCCT GGTGACTGCC ATCAGCAAAG CTTCAGCGCA GCAAAGAGCT 
GGTCGTGCAG GTCGTACCCG GCCTGGCAAG TGCTTCCGGC TTTATACAGA AAAGGCTTAC 
AAAACAGAAA TGCAGGACAA TACTTACCCT GAAATATTGA GGTCCAATTT GGGTTCCGTA 
GTATTGCAGT TAAAGAAACT TGGCATTGAT GACTTGGTTC ATTTTGATTT CATGGACCCT 
CCAGCGCCTG AAACTTTAAT GCGTGCCCTG GAGCTTTTGA ACTACCTGGC TGCCTTAAAT 
GACGACGGAG ACCTGACAGA ATTGGGATCT ATGATGGCAG AGTTCCCACT GGACCCACAG 
CTGGCCAAGA TGGTTATTGC CAGTTGTGAC TACAACTGCT CTAATGAGAT CTTATCCATA 
ACAGCCATGT TGTCAGTCCC ACAGTGTTTC ATTCGACCCA CGGAAGTCAA GAAAGCTGCC 
GATGAGGCTA AGATGAGGTT TGCCCACATC GACGGTGATC ATTTGACGCT GCTGAATGTA 
TACCATGCTT TCAAGCAGAA TCACGAGTCG TCTCAGTGGT GTTATGACAA CTTCATTAAC 
TACAGATCCC TGATGTCTGC AGACAATGTA CGGCAGCAGC TGTCACGAAT CATGGACAGA 
TTTAACTTGC CGCGAAGAAG CACAGACTTT ACAAGCCGAG ATTATTACAT TAATATTAGG 
AAAGCTTTGG TTACTGGTTA TTTTATGCAG GTGGCACACT TGGAGCGAAC TGGTCACTAT 
CTAACAGTGA AAGACAACCA AGTGGTACAG TTGCACCCGT CAACCGTTCT TGACCACAAA 
CCCGAGTGGG TCCTGTACAA CGAGTTTGTC CTCACAACCA AGAACTACAT CCGCACATGC 
ACAGATATAA AGCCTGAATG GTTGGTGAAA ATTGCAGCTC AATATTACGA CATGAGCAAC 
TTTCCTCAGT GTGAAGCAAA GAGGCAACTG GAGCGCATTG TGGCCAAACT TCAGACTAAG 
GAATATTCAC AGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dhx15 ( XM_018091366.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946855.1 Xenopus tropicalis DEAH-box helicase 15 (dhx15), transcript variant X2, mRNA.

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CloneID	OXa41716
Clone ID Related Accession (Same CDS sequence)	XM_018091366.1
Accession Version	XM_018091366.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCTC TAAAACCTGC AGCGGTTGCT CAAGGCATTA ATCCCTTTAC CAACTTACCC 
CACACTCCGC GCTACTATGA CATCTTAAAA AAGAGGCTAC TGTTGCCAGT ATGGGAATAC 
AAAGAACGGT TTACTGAAAT TCTTATGAAG AACCAGAGCT TTGTATTGGT TGGTGAGACT 
GGATCTGGGA AAACTACTCA GATTCCACAG TGGTGTGTCG ACTACATGAG GTCATTGCCT 
GGTCCCAAAA GAGGCGTTGC ATGTACCCAG CCAAGAAGAG TGGCTGCCAT GAGTGTGGCT 
CAAAGAGTTG CAGATGAAAT GGATGTCATG TTGGGTCAGG AAGTTGGTTA TTCCATTAGG 
TTTGAAGACT GCAGCAGTGC AAAAACAATC TTGAAGTACA TGACAGACGG TATGTTACTC 
CGGGAAGCTA TGAACGATCC ATTGTTGGAA CGCTATGGGG TAGTGATTCT TGACGAAGCT 
CATGAAAGGA CCTTAGCAAC AGATATTCTA ATGGGTGTTT TAAAGGAAGT TGTAAGACAA 
AGGAATGACT TAAAGGTTAT AGTTATGAGT GCCACTTTAG ATGCTGGCAA ATTCCAGGTA 
TATTTTGATA GCTGCCCTCT TTTAACAATC CCTGGACGAA CACACCCTGT AGAAATCTTC 
TATACTCCAG AGCCTGAGCG GGATTACCTA GAGGCAGCCA TTCGAACAGT GATTCAGATT 
CACATGTGTG AAGAGGAGGA AGGAGATCTC CTGCTTTTTC TTACTGGACA AGAGGAAATT 
GACGAGGCCT GCAAGAGAAT CAAACGGGAA ATCGATGACT TGGGCCCTGA TGTAGGAGAC 
ATCAAAATCA TTCCTTTATA TTCAACCCTC CCACCTCAGC AACAGCAAAG AATATTTGAG 
CCTCCTCCAC CCAAGAAACC TAGTGGAGCA ATAGGAAGGA AAGTTGTAGT TTCCACTAAC 
ATCGCTGAAA CATCTCTGAC TATAGATGGT GTTGTGTTTG TGATCGATCC TGGCTTTGCC 
AAGCAAAAGG TGTATAACCC TCGAATTCGA GTGGAATCTC TCCTGGTGAC TGCCATCAGC 
AAAGCTTCAG CGCAGCAAAG AGCTGGTCGT GCAGGTCGTA CCCGGCCTGG CAAGTGCTTC 
CGGCTTTATA CAGAAAAGGC TTACAAAACA GAAATGCAGG ACAATACTTA CCCTGAAATA 
TTGAGGTCCA ATTTGGGTTC CGTAGTATTG CAGTTAAAGA AACTTGGCAT TGATGACTTG 
GTTCATTTTG ATTTCATGGA CCCTCCAGCG CCTGAAACTT TAATGCGTGC CCTGGAGCTT 
TTGAACTACC TGGCTGCCTT AAATGACGAC GGAGACCTGA CAGAATTGGG ATCTATGATG 
GCAGAGTTCC CACTGGACCC ACAGCTGGCC AAGATGGTTA TTGCCAGTTG TGACTACAAC 
TGCTCTAATG AGATCTTATC CATAACAGCC ATGTTGTCAG TCCCACAGTG TTTCATTCGA 
CCCACGGAAG TCAAGAAAGC TGCCGATGAG GCTAAGATGA GGTTTGCCCA CATCGACGGT 
GATCATTTGA CGCTGCTGAA TGTATACCAT GCTTTCAAGC AGAATCACGA GTCGTCTCAG 
TGGTGTTATG ACAACTTCAT TAACTACAGA TCCCTGATGT CTGCAGACAA TGTACGGCAG 
CAGCTGTCAC GAATCATGGA CAGATTTAAC TTGCCGCGAA GAAGCACAGA CTTTACAAGC 
CGAGATTATT ACATTAATAT TAGGAAAGCT TTGGTTACTG GTTATTTTAT GCAGGTGGCA 
CACTTGGAGC GAACTGGTCA CTATCTAACA GTGAAAGACA ACCAAGTGGT ACAGTTGCAC 
CCGTCAACCG TTCTTGACCA CAAACCCGAG TGGGTCCTGT ACAACGAGTT TGTCCTCACA 
ACCAAGAACT ACATCCGCAC ATGCACAGAT ATAAAGCCTG AATGGTTGGT GAAAATTGCA 
GCTCAATATT ACGACATGAG CAACTTTCCT CAGTGTGAAG CAAAGAGGCA ACTGGAGCGC 
ATTGTGGCCA AACTTCAGAC TAAGGAATAT TCACAGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946855.1
CDS	392..2473
Translation	MPPLKPAAVAQGINPFTNLPHTPRYYDILKKRLLLPVWEYKERFTEILMKNQSFVLVGETGSGKTTQIPQWCVDYMRSLPGPKRGVACTQPRRVAAMSVAQRVADEMDVMLGQEVGYSIRFEDCSSAKTILKYMTDGMLLREAMNDPLLERYGVVILDEAHERTLATDILMGVLKEVVRQRNDLKVIVMSATLDAGKFQVYFDSCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHMCEEEEGDLLLFLTGQEEIDEACKRIKREIDDLGPDVGDIKIIPLYSTLPPQQQQRIFEPPPPKKPSGAIGRKVVVSTNIAETSLTIDGVVFVIDPGFAKQKVYNPRIRVESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAYKTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALNDDGDLTELGSMMAEFPLDPQLAKMVIASCDYNCSNEILSITAMLSVPQCFIRPTEVKKAADEAKMRFAHIDGDHLTLLNVYHAFKQNHESSQWCYDNFINYRSLMSADNVRQQLSRIMDRFNLPRRSTDFTSRDYYINIRKALVTGYFMQVAHLERTGHYLTVKDNQVVQLHPSTVLDHKPEWVLYNEFVLTTKNYIRTCTDIKPEWLVKIAAQYYDMSNFPQCEAKRQLERIVAKLQTKEYSQY

Target ORF information:

RefSeq Version	XM_018091366.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAH-box helicase 15 (dhx15), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091366.1

ORF Insert Sequence:

ATGCCACCTC TAAAACCTGC AGCGGTTGCT CAAGGCATTA ATCCCTTTAC CAACTTACCC 
CACACTCCGC GCTACTATGA CATCTTAAAA AAGAGGCTAC TGTTGCCAGT ATGGGAATAC 
AAAGAACGGT TTACTGAAAT TCTTATGAAG AACCAGAGCT TTGTATTGGT TGGTGAGACT 
GGATCTGGGA AAACTACTCA GATTCCACAG TGGTGTGTCG ACTACATGAG GTCATTGCCT 
GGTCCCAAAA GAGGCGTTGC ATGTACCCAG CCAAGAAGAG TGGCTGCCAT GAGTGTGGCT 
CAAAGAGTTG CAGATGAAAT GGATGTCATG TTGGGTCAGG AAGTTGGTTA TTCCATTAGG 
TTTGAAGACT GCAGCAGTGC AAAAACAATC TTGAAGTACA TGACAGACGG TATGTTACTC 
CGGGAAGCTA TGAACGATCC ATTGTTGGAA CGCTATGGGG TAGTGATTCT TGACGAAGCT 
CATGAAAGGA CCTTAGCAAC AGATATTCTA ATGGGTGTTT TAAAGGAAGT TGTAAGACAA 
AGGAATGACT TAAAGGTTAT AGTTATGAGT GCCACTTTAG ATGCTGGCAA ATTCCAGGTA 
TATTTTGATA GCTGCCCTCT TTTAACAATC CCTGGACGAA CACACCCTGT AGAAATCTTC 
TATACTCCAG AGCCTGAGCG GGATTACCTA GAGGCAGCCA TTCGAACAGT GATTCAGATT 
CACATGTGTG AAGAGGAGGA AGGAGATCTC CTGCTTTTTC TTACTGGACA AGAGGAAATT 
GACGAGGCCT GCAAGAGAAT CAAACGGGAA ATCGATGACT TGGGCCCTGA TGTAGGAGAC 
ATCAAAATCA TTCCTTTATA TTCAACCCTC CCACCTCAGC AACAGCAAAG AATATTTGAG 
CCTCCTCCAC CCAAGAAACC TAGTGGAGCA ATAGGAAGGA AAGTTGTAGT TTCCACTAAC 
ATCGCTGAAA CATCTCTGAC TATAGATGGT GTTGTGTTTG TGATCGATCC TGGCTTTGCC 
AAGCAAAAGG TGTATAACCC TCGAATTCGA GTGGAATCTC TCCTGGTGAC TGCCATCAGC 
AAAGCTTCAG CGCAGCAAAG AGCTGGTCGT GCAGGTCGTA CCCGGCCTGG CAAGTGCTTC 
CGGCTTTATA CAGAAAAGGC TTACAAAACA GAAATGCAGG ACAATACTTA CCCTGAAATA 
TTGAGGTCCA ATTTGGGTTC CGTAGTATTG CAGTTAAAGA AACTTGGCAT TGATGACTTG 
GTTCATTTTG ATTTCATGGA CCCTCCAGCG CCTGAAACTT TAATGCGTGC CCTGGAGCTT 
TTGAACTACC TGGCTGCCTT AAATGACGAC GGAGACCTGA CAGAATTGGG ATCTATGATG 
GCAGAGTTCC CACTGGACCC ACAGCTGGCC AAGATGGTTA TTGCCAGTTG TGACTACAAC 
TGCTCTAATG AGATCTTATC CATAACAGCC ATGTTGTCAG TCCCACAGTG TTTCATTCGA 
CCCACGGAAG TCAAGAAAGC TGCCGATGAG GCTAAGATGA GGTTTGCCCA CATCGACGGT 
GATCATTTGA CGCTGCTGAA TGTATACCAT GCTTTCAAGC AGAATCACGA GTCGTCTCAG 
TGGTGTTATG ACAACTTCAT TAACTACAGA TCCCTGATGT CTGCAGACAA TGTACGGCAG 
CAGCTGTCAC GAATCATGGA CAGATTTAAC TTGCCGCGAA GAAGCACAGA CTTTACAAGC 
CGAGATTATT ACATTAATAT TAGGAAAGCT TTGGTTACTG GTTATTTTAT GCAGGTGGCA 
CACTTGGAGC GAACTGGTCA CTATCTAACA GTGAAAGACA ACCAAGTGGT ACAGTTGCAC 
CCGTCAACCG TTCTTGACCA CAAACCCGAG TGGGTCCTGT ACAACGAGTT TGTCCTCACA 
ACCAAGAACT ACATCCGCAC ATGCACAGAT ATAAAGCCTG AATGGTTGGT GAAAATTGCA 
GCTCAATATT ACGACATGAG CAACTTTCCT CAGTGTGAAG CAAAGAGGCA ACTGGAGCGC 
ATTGTGGCCA AACTTCAGAC TAAGGAATAT TCACAGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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dhx15 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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