Gene Symbol | PRP43 |
Entrez Gene ID | 852757 |
Full Name | DEAH-box ATP-dependent RNA helicase PRP43 |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
ORF » Species Summary » Saccharomyces cerevisiae S288C » PRP43 cDNA ORF clone
Gene Symbol | PRP43 |
Entrez Gene ID | 852757 |
Full Name | DEAH-box ATP-dependent RNA helicase PRP43 |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
mRNA | Protein | Name |
---|---|---|
NM_001180985.1 | NP_011395.1 | DEAH-box ATP-dependent RNA helicase PRP43 |
Mus musculus (house mouse) | Dhx15 | NP_031865.2 |
Saccharomyces cerevisiae (baker's yeast) | PRP43 | NP_011395.1 |
Schizosaccharomyces pombe (fission yeast) | prp43 | NP_595937.1 |
Oryza sativa (rice) | Os03g0314100 | NP_001049931.1 |
Rattus norvegicus (Norway rat) | Dhx15 | NP_001178526.1 |
Gallus gallus (chicken) | DHX15 | NP_001026330.1 |
Danio rerio (zebrafish) | dhx15 | NP_001108613.1 |
Kluyveromyces lactis | KLLA0B00561g | XP_451555.1 |
Magnaporthe oryzae (rice blast fungus) | MGG_03893 | XP_003719983.1 |
Xenopus tropicalis (tropical clawed frog) | dhx15 | NP_001135571.1 |
DHX15 | XP_001106067.1 | |
Bos taurus (cattle) | DHX15 | NP_001091487.1 |
Anopheles gambiae (African malaria mosquito) | AgaP_AGAP011149 | XP_309498.4 |
Caenorhabditis elegans (roundworm) | F56D2.6 | NP_741147.1 |
Homo sapiens (human) | DHX15 | NP_001349.2 |
Drosophila melanogaster (fruit fly) | CG11107 | NP_610269.1 |
Arabidopsis thaliana (thale cress) | AT3G62310 | NP_191790.1 |
Pan troglodytes (chimpanzee) | DHX15 | XP_001164599.1 |
Canis lupus familiaris (dog) | DHX15 | XP_851535.1 |
Eremothecium gossypii | AGOS_AAR180C | NP_982723.1 |
Neurospora crassa | NCU01612 | XP_956523.2 |
Arabidopsis thaliana (thale cress) | AT2G47250 | NP_182247.1 |
The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)
Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Study shows the crystal structure of Prp43p bound to single-stranded poly-uracil RNA and reveals differences with viral ATPases in the RecA1 domain but with striking similarities with viral ATPases in the RecA2 domain of the catalytic core, including both a prominent beta-hairpin that bookends the 5' end of the RNA stack and a dynamic motif Va that is implicated in mediating translocation.
Title: Structure of the DEAH/RHA ATPase Prp43p bound to RNA implicates a pair of hairpins and motif Va in translocation along RNA.
Ntr1 and Ntr2 may act as 'doorkeepers' and both cofactors may inspect the RNP structure of spliceosomal complexes thereby targeting suboptimal spliceosomes for Prp43-mediated disassembly.
Title: Regulation of Prp43-mediated disassembly of spliceosomes by its cofactors Ntr1 and Ntr2.
Using Prp43 F357A mutants or pyrimidine nucleotides, we show that the lack of stacking of the nucleotide base to the F-motif decouples the NTPase and helicase activities of Prp43. In contrast the R159A mutant (R-motif) showed reduced ATPase and helicase activities.
Title: Functional link between DEAH/RHA helicase Prp43 activation and ATP base binding.
These data indicate that the U2 snRNP-intron interaction is Prp43's major target.
Title: The target of the DEAH-box NTP triphosphatase Prp43 in Saccharomyces cerevisiae spliceosomes is the U2 snRNP-intron interaction.
Results show that Prp43 expression is regulated by Cmg1 which stimulates its RNA helicase activity.
Title: Protein cofactor competition regulates the action of a multifunctional RNA helicase in different pathways.
The following PRP43 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRP43 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OSi02128 | |
Clone ID Related Accession (Same CDS sequence) | NM_001180985.1 | |
Accession Version | NM_001180985.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-10-31 | |
Organism | Saccharomyces cerevisiae S288C | |
Product | DEAH-box ATP-dependent RNA helicase PRP43 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. |
1 | ATGGGTTCCA AAAGAAGATT CTCGTCCGAA CACCCGGATC CAGTTGAGAC CTCTATTCCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_011395.1 |
CDS | 1..2304 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001180985.1 |
1 | ATGGGTTCCA AAAGAAGATT CTCGTCCGAA CACCCGGATC CAGTTGAGAC CTCTATTCCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The nucleotide sequence of Saccharomyces cerevisiae chromosome VII. |
Life with 6000 genes. |