Dhx15 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dhx15
Entrez Gene ID	13204
Full Name	DEAH (Asp-Glu-Ala-His) box polypeptide 15
Synonyms	DBP1,DEAH9,Ddx15,HRH2,mDEAH9
General protein information	Preferred Names DEAH (Asp-Glu-Ala-His) box polypeptide 15 Names pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 15 (RNA helicase A) DEAH box protein 15 putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_007839.3	NP_031865.2	pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 isoform 2
NM_001042620.2	NP_001036085.1	pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Dhx15	NP_031865.2
Saccharomyces cerevisiae (baker's yeast)	PRP43	NP_011395.1
Schizosaccharomyces pombe (fission yeast)	prp43	NP_595937.1
Oryza sativa (rice)	Os03g0314100	NP_001049931.1
Rattus norvegicus (Norway rat)	Dhx15	NP_001178526.1
Gallus gallus (chicken)	DHX15	NP_001026330.1
Danio rerio (zebrafish)	dhx15	NP_001108613.1
Kluyveromyces lactis	KLLA0B00561g	XP_451555.1
Magnaporthe oryzae (rice blast fungus)	MGG_03893	XP_003719983.1
Xenopus tropicalis (tropical clawed frog)	dhx15	NP_001135571.1
	DHX15	XP_001106067.1
Bos taurus (cattle)	DHX15	NP_001091487.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011149	XP_309498.4
Caenorhabditis elegans (roundworm)	F56D2.6	NP_741147.1
Homo sapiens (human)	DHX15	NP_001349.2
Drosophila melanogaster (fruit fly)	CG11107	NP_610269.1
Arabidopsis thaliana (thale cress)	AT3G62310	NP_191790.1
Pan troglodytes (chimpanzee)	DHX15	XP_001164599.1
Canis lupus familiaris (dog)	DHX15	XP_851535.1
Eremothecium gossypii	AGOS_AAR180C	NP_982723.1
Neurospora crassa	NCU01612	XP_956523.2
Arabidopsis thaliana (thale cress)	AT2G47250	NP_182247.1

Gene Ontology
Bibliography

Related articles in PubMed

Conserved expression of ultra-conserved noncoding RNA in mammalian nervous system.
Zhou J, Wang R, Zhang J, Zhu L, Liu W, Lu S, Chen P, Li H, Yin B, Yuan J, Qiang B, Shu P, Peng X
Biochimica et biophysica acta1860(12)1159-1168(2017 Dec)

Nlrp6 regulates intestinal antiviral innate immunity.
Wang P, Zhu S, Yang L, Cui S, Pan W, Jackson R, Zheng Y, Rongvaux A, Sun Q, Yang G, Gao S, Lin R, You F, Flavell R, Fikrig E
Science (New York, N.Y.)350(6262)826-30(2015 Nov)

The Regulatory Factor ZFHX3 Modifies Circadian Function in SCN via an AT Motif-Driven Axis.
Parsons MJ, Brancaccio M, Sethi S, Maywood ES, Satija R, Edwards JK, Jagannath A, Couch Y, Finelli MJ, Smyllie NJ, Esapa C, Butler R, Barnard AR, Chesham JE, Saito S, Joynson G, Wells S, Foster RG, Oliver PL, Simon MM, Mallon AM, Hastings MH, Nolan PM
Cell162(3)607-21(2015 Jul)

DHX15 senses double-stranded RNA in myeloid dendritic cells.
Lu H, Lu N, Weng L, Yuan B, Liu YJ, Zhang Z
Journal of immunology (Baltimore, Md. : 1950)193(3)1364-72(2014 Aug)

Raftlin is involved in the nucleocapture complex to induce poly(I:C)-mediated TLR3 activation.
Watanabe A, Tatematsu M, Saeki K, Shibata S, Shime H, Yoshimura A, Obuse C, Seya T, Matsumoto M
The Journal of biological chemistry286(12)10702-11(2011 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data demonstrate that Nlrp6 functions with Dhx15 as a viral RNA sensor to induce IFN-stimulated genes, and this effect is especially important in the intestinal tract.
Title: Nlrp6 regulates intestinal antiviral innate immunity.

DHX15, a DEXDc helicase family member, plays an important role in sensing RNA and RNA viruses in bone marrow dendritic cells.
Title: DHX15 senses double-stranded RNA in myeloid dendritic cells.

The following Dhx15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dhx15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18279	NM_001042620.2 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 15 (Dhx15), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu18452	NM_007839.3 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 15 (Dhx15), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$468.30 $669.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dhx15 ( NM_001042620.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001036085.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 15 (Dhx15), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18279
Clone ID Related Accession (Same CDS sequence)	NM_001042620.2
Accession Version	NM_001042620.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY285872.1, AK149869.1 and AK043111.1. On Oct 1, 2013 this sequence version replaced NM_001042620.1. Transcript Variant: This variant (1) contains an alternate 3' terminal exon, resulting in a different 3' UTR and 3' coding region, compared to variant 2. It encodes isoform 1, which has a shorter and distinct C-terminus, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC100343.1, SRR1660813.65361.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGTCCAAGA GGCATCGGTT GGACCTGGGG GAGGATTACC CCTCCGGCAA GAAGCGTGCG 
GGGACCGATG GGAAAGACCG GGAACGAGAT CGGGATAGAG AGGACCGATC TAAAGATCGT 
GACCGAGAAC GTGATAGAGG AGACAGAGAA CGGGAGAGGG AAAAAGAAAA GGAAAAAGAA 
TTGAGAGCTT CCACCAATGC TATGCTTATC AGTGCTGGAT TGCCACCTTT AAAAGCTTCT 
CATTCAGCTC ACTCAACCCA CTCGGCTCAT TCAACACATT CTACACATTC TGCCCACTCA 
ACACACACTG GACACACAGG ACACACATCA CTTCCACAGT GCATTAATCC ATTCACCAAC 
CTGCCCCATA CTCCTCGATA CTATGATATT CTAAAGAAAC GACTTCAGCT CCCTGTATGG 
GAATACAAGG ATAGATTTAC AGATATTCTT GTTAGACATC AGTCATTTGT ACTTGTTGGT 
GAGACTGGGT CTGGTAAAAC AACACAGATA CCACAGTGGT GTGTGGAGTA TATGCGATCC 
TTGCCAGGAC CCAAAAGAGG AGTCGCCTGT ACACAGCCCA GGAGAGTGGC TGCAATGAGT 
GTGGCTCAGA GAGTTGCTGA TGAGATGGAC GTGATGTTAG GCCAGGAAGT TGGATACTCC 
ATTCGATTTG AAGACTGCAG TAGTGCAAAA ACCATTCTTA AGTATATGAC TGACGGGATG 
CTACTTCGTG AAGCCATGAA CGATCCCCTT CTGGAGCGTT ATGGTGTGAT AATTCTTGAT 
GAGGCCCATG AAAGAACATT GGCTACAGAT ATTCTCATGG GTGTTCTAAA GGAAGTTGTA 
AGACAGAGAT CAGATTTAAA GGTTATAGTT ATGAGTGCTA CCCTAGATGC TGGGAAATTC 
CAGATTTACT TTGATAACTG TCCTCTCTTA ACTATTCCTG GCCGAACACA TCCTGTTGAG 
ATTTTTTATA CTCCAGAACC AGAGAGAGAT TATCTTGAAG CAGCAATTCG GACAGTGATC 
CAAATACATA TGTGTGAAGA GGAGGAAGGA GACCTTCTTC TTTTCTTGAC TGGTCAAGAG 
GAAATTGATG AGGCCTGTAA GAGAATAAAG CGTGAAGTTG ATGATTTGGG CCCTGAAGTT 
GGTGATATTA AAATCATTCC ATTATATTCT ACACTTCCAC CCCAGCAGCA ACAACGCATT 
TTTGAGCCAC CACCTCCAAA AAAACAGAAT GGAGCAATTG GAAGAAAGGT GGTGGTGTCA 
ACTAATATTG CAGAGACCTC TTTGACAATA GACGGTGTGG TGTTTGTAAT TGACCCTGGA 
TTTGCAAAAC AAAAGGTATA TAATCCTAGA ATCAGAGTCG AGTCTCTTCT GGTGACTGCC 
ATTAGTAAAG CTTCAGCTCA GCAAAGGGCG GGTCGAGCTG GACGGACCAG ACCTGGGAAA 
TGCTTCAGGC TTTATACAGA GAAAGCTTAT AAAACAGAAA TGCAGGACAA CACCTATCCT 
GAGATCCTGC GCTCTAACCT AGGATCGGTT GTGTTGCAGC TGAAGAAGCT TGGTATTGAT 
GACTTGGTAC ACTTTGATTT TATGGACCCA CCAGCTCCTG AAACTCTGAT GAGAGCCCTA 
GAACTTTTGA ATTATTTGGC TGCTTTAAAT GATGATGGAG ATCTGACTGA ATTGGGATCC 
ATGATGGCAG AGTTCCCTTT AGATCCACAG CTCGCTAAAA TGGTTATTGC AAGTTGTGAC 
TACAACTGTT CTAATGAGGT CCTATCTATT ACTGCTATGT TGTCAGTCCC ACAGTGTTTT 
GTTCGCCCCA CGGAGGCCAA GAAAGCTGCA GATGAGGCCA AGATGAGATT TGCCCACATA 
GATGGAGATC ATCTGACACT GCTGAATGTC TACCACGCTT TTAAACAAAA TCATGAATCT 
GTTCAGTGGT GTTATGACAA CTTCATTAAC TACCGGTCCC TGATGTCTGC AGACAATGTA 
CGCCAGCAGC TATCAAGAAT TATGGACAGA TTTAATTTGC CTCGTCGAAG TACTGATTTC 
ACAAGCAGGG ACTATTATAT TAATATAAGA AAAGCTTTGG TTACTGGGTA TTTTATGCAA 
GTAAGTAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036085.1
CDS	197..2308
Translation	MSKRHRLDLGEDYPSGKKRAGTDGKDRERDRDREDRSKDRDRERDRGDREREREKEKEKELRASTNAMLISAGLPPLKASHSAHSTHSAHSTHSTHSAHSTHTGHTGHTSLPQCINPFTNLPHTPRYYDILKKRLQLPVWEYKDRFTDILVRHQSFVLVGETGSGKTTQIPQWCVEYMRSLPGPKRGVACTQPRRVAAMSVAQRVADEMDVMLGQEVGYSIRFEDCSSAKTILKYMTDGMLLREAMNDPLLERYGVIILDEAHERTLATDILMGVLKEVVRQRSDLKVIVMSATLDAGKFQIYFDNCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHMCEEEEGDLLLFLTGQEEIDEACKRIKREVDDLGPEVGDIKIIPLYSTLPPQQQQRIFEPPPPKKQNGAIGRKVVVSTNIAETSLTIDGVVFVIDPGFAKQKVYNPRIRVESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAYKTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALNDDGDLTELGSMMAEFPLDPQLAKMVIASCDYNCSNEVLSITAMLSVPQCFVRPTEAKKAADEAKMRFAHIDGDHLTLLNVYHAFKQNHESVQWCYDNFINYRSLMSADNVRQQLSRIMDRFNLPRRSTDFTSRDYYINIRKALVTGYFMQVSS

Target ORF information:

RefSeq Version	NM_001042620.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 15 (Dhx15), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042620.2

ORF Insert Sequence:

ATGTCCAAGA GGCATCGGTT GGACCTGGGG GAGGATTACC CCTCCGGCAA GAAGCGTGCG 
GGGACCGATG GGAAAGACCG GGAACGAGAT CGGGATAGAG AGGACCGATC TAAAGATCGT 
GACCGAGAAC GTGATAGAGG AGACAGAGAA CGGGAGAGGG AAAAAGAAAA GGAAAAAGAA 
TTGAGAGCTT CCACCAATGC TATGCTTATC AGTGCTGGAT TGCCACCTTT AAAAGCTTCT 
CATTCAGCTC ACTCAACCCA CTCGGCTCAT TCAACACATT CTACACATTC TGCCCACTCA 
ACACACACTG GACACACAGG ACACACATCA CTTCCACAGT GCATTAATCC ATTCACCAAC 
CTGCCCCATA CTCCTCGATA CTATGATATT CTAAAGAAAC GACTTCAGCT CCCTGTATGG 
GAATACAAGG ATAGATTTAC AGATATTCTT GTTAGACATC AGTCATTTGT ACTTGTTGGT 
GAGACTGGGT CTGGTAAAAC AACACAGATA CCACAGTGGT GTGTGGAGTA TATGCGATCC 
TTGCCAGGAC CCAAAAGAGG AGTCGCCTGT ACACAGCCCA GGAGAGTGGC TGCAATGAGT 
GTGGCTCAGA GAGTTGCTGA TGAGATGGAC GTGATGTTAG GCCAGGAAGT TGGATACTCC 
ATTCGATTTG AAGACTGCAG TAGTGCAAAA ACCATTCTTA AGTATATGAC TGACGGGATG 
CTACTTCGTG AAGCCATGAA CGATCCCCTT CTGGAGCGTT ATGGTGTGAT AATTCTTGAT 
GAGGCCCATG AAAGAACATT GGCTACAGAT ATTCTCATGG GTGTTCTAAA GGAAGTTGTA 
AGACAGAGAT CAGATTTAAA GGTTATAGTT ATGAGTGCTA CCCTAGATGC TGGGAAATTC 
CAGATTTACT TTGATAACTG TCCTCTCTTA ACTATTCCTG GCCGAACACA TCCTGTTGAG 
ATTTTTTATA CTCCAGAACC AGAGAGAGAT TATCTTGAAG CAGCAATTCG GACAGTGATC 
CAAATACATA TGTGTGAAGA GGAGGAAGGA GACCTTCTTC TTTTCTTGAC TGGTCAAGAG 
GAAATTGATG AGGCCTGTAA GAGAATAAAG CGTGAAGTTG ATGATTTGGG CCCTGAAGTT 
GGTGATATTA AAATCATTCC ATTATATTCT ACACTTCCAC CCCAGCAGCA ACAACGCATT 
TTTGAGCCAC CACCTCCAAA AAAACAGAAT GGAGCAATTG GAAGAAAGGT GGTGGTGTCA 
ACTAATATTG CAGAGACCTC TTTGACAATA GACGGTGTGG TGTTTGTAAT TGACCCTGGA 
TTTGCAAAAC AAAAGGTATA TAATCCTAGA ATCAGAGTCG AGTCTCTTCT GGTGACTGCC 
ATTAGTAAAG CTTCAGCTCA GCAAAGGGCG GGTCGAGCTG GACGGACCAG ACCTGGGAAA 
TGCTTCAGGC TTTATACAGA GAAAGCTTAT AAAACAGAAA TGCAGGACAA CACCTATCCT 
GAGATCCTGC GCTCTAACCT AGGATCGGTT GTGTTGCAGC TGAAGAAGCT TGGTATTGAT 
GACTTGGTAC ACTTTGATTT TATGGACCCA CCAGCTCCTG AAACTCTGAT GAGAGCCCTA 
GAACTTTTGA ATTATTTGGC TGCTTTAAAT GATGATGGAG ATCTGACTGA ATTGGGATCC 
ATGATGGCAG AGTTCCCTTT AGATCCACAG CTCGCTAAAA TGGTTATTGC AAGTTGTGAC 
TACAACTGTT CTAATGAGGT CCTATCTATT ACTGCTATGT TGTCAGTCCC ACAGTGTTTT 
GTTCGCCCCA CGGAGGCCAA GAAAGCTGCA GATGAGGCCA AGATGAGATT TGCCCACATA 
GATGGAGATC ATCTGACACT GCTGAATGTC TACCACGCTT TTAAACAAAA TCATGAATCT 
GTTCAGTGGT GTTATGACAA CTTCATTAAC TACCGGTCCC TGATGTCTGC AGACAATGTA 
CGCCAGCAGC TATCAAGAAT TATGGACAGA TTTAATTTGC CTCGTCGAAG TACTGATTTC 
ACAAGCAGGG ACTATTATAT TAATATAAGA AAAGCTTTGG TTACTGGGTA TTTTATGCAA 
GTAAGTAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx15 ( NM_007839.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031865.2 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 15 (Dhx15), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu18452
Clone ID Related Accession (Same CDS sequence)	NM_007839.3
Accession Version	NM_007839.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Mus musculus(house mouse)
Product	pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY285872.1, AK149869.1, AK145983.1 and BP767627.1. On Oct 1, 2013 this sequence version replaced NM_007839.2. Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC003745.1, AK168835.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAAGA GGCATCGGTT GGACCTGGGG GAGGATTACC CCTCCGGCAA GAAGCGTGCG 
GGGACCGATG GGAAAGACCG GGAACGAGAT CGGGATAGAG AGGACCGATC TAAAGATCGT 
GACCGAGAAC GTGATAGAGG AGACAGAGAA CGGGAGAGGG AAAAAGAAAA GGAAAAAGAA 
TTGAGAGCTT CCACCAATGC TATGCTTATC AGTGCTGGAT TGCCACCTTT AAAAGCTTCT 
CATTCAGCTC ACTCAACCCA CTCGGCTCAT TCAACACATT CTACACATTC TGCCCACTCA 
ACACACACTG GACACACAGG ACACACATCA CTTCCACAGT GCATTAATCC ATTCACCAAC 
CTGCCCCATA CTCCTCGATA CTATGATATT CTAAAGAAAC GACTTCAGCT CCCTGTATGG 
GAATACAAGG ATAGATTTAC AGATATTCTT GTTAGACATC AGTCATTTGT ACTTGTTGGT 
GAGACTGGGT CTGGTAAAAC AACACAGATA CCACAGTGGT GTGTGGAGTA TATGCGATCC 
TTGCCAGGAC CCAAAAGAGG AGTCGCCTGT ACACAGCCCA GGAGAGTGGC TGCAATGAGT 
GTGGCTCAGA GAGTTGCTGA TGAGATGGAC GTGATGTTAG GCCAGGAAGT TGGATACTCC 
ATTCGATTTG AAGACTGCAG TAGTGCAAAA ACCATTCTTA AGTATATGAC TGACGGGATG 
CTACTTCGTG AAGCCATGAA CGATCCCCTT CTGGAGCGTT ATGGTGTGAT AATTCTTGAT 
GAGGCCCATG AAAGAACATT GGCTACAGAT ATTCTCATGG GTGTTCTAAA GGAAGTTGTA 
AGACAGAGAT CAGATTTAAA GGTTATAGTT ATGAGTGCTA CCCTAGATGC TGGGAAATTC 
CAGATTTACT TTGATAACTG TCCTCTCTTA ACTATTCCTG GCCGAACACA TCCTGTTGAG 
ATTTTTTATA CTCCAGAACC AGAGAGAGAT TATCTTGAAG CAGCAATTCG GACAGTGATC 
CAAATACATA TGTGTGAAGA GGAGGAAGGA GACCTTCTTC TTTTCTTGAC TGGTCAAGAG 
GAAATTGATG AGGCCTGTAA GAGAATAAAG CGTGAAGTTG ATGATTTGGG CCCTGAAGTT 
GGTGATATTA AAATCATTCC ATTATATTCT ACACTTCCAC CCCAGCAGCA ACAACGCATT 
TTTGAGCCAC CACCTCCAAA AAAACAGAAT GGAGCAATTG GAAGAAAGGT GGTGGTGTCA 
ACTAATATTG CAGAGACCTC TTTGACAATA GACGGTGTGG TGTTTGTAAT TGACCCTGGA 
TTTGCAAAAC AAAAGGTATA TAATCCTAGA ATCAGAGTCG AGTCTCTTCT GGTGACTGCC 
ATTAGTAAAG CTTCAGCTCA GCAAAGGGCG GGTCGAGCTG GACGGACCAG ACCTGGGAAA 
TGCTTCAGGC TTTATACAGA GAAAGCTTAT AAAACAGAAA TGCAGGACAA CACCTATCCT 
GAGATCCTGC GCTCTAACCT AGGATCGGTT GTGTTGCAGC TGAAGAAGCT TGGTATTGAT 
GACTTGGTAC ACTTTGATTT TATGGACCCA CCAGCTCCTG AAACTCTGAT GAGAGCCCTA 
GAACTTTTGA ATTATTTGGC TGCTTTAAAT GATGATGGAG ATCTGACTGA ATTGGGATCC 
ATGATGGCAG AGTTCCCTTT AGATCCACAG CTCGCTAAAA TGGTTATTGC AAGTTGTGAC 
TACAACTGTT CTAATGAGGT CCTATCTATT ACTGCTATGT TGTCAGTCCC ACAGTGTTTT 
GTTCGCCCCA CGGAGGCCAA GAAAGCTGCA GATGAGGCCA AGATGAGATT TGCCCACATA 
GATGGAGATC ATCTGACACT GCTGAATGTC TACCACGCTT TTAAACAAAA TCATGAATCT 
GTTCAGTGGT GTTATGACAA CTTCATTAAC TACCGGTCCC TGATGTCTGC AGACAATGTA 
CGCCAGCAGC TATCAAGAAT TATGGACAGA TTTAATTTGC CTCGTCGAAG TACTGATTTC 
ACAAGCAGGG ACTATTATAT TAATATAAGA AAAGCTTTGG TTACTGGGTA TTTTATGCAA 
GTGGCACATT TAGAACGAAC GGGGCATTAT TTAACTGTGA AAGATAACCA GGTGGTCCAG 
TTGCATCCCT CTACTGTGCT TGATCACAAG CCTGAATGGG TGCTTTATAA TGAGTTTGTG 
CTGACGACAA AGAATTATAT CCGGACATGT ACAGATATCA AGCCAGAATG GTTGGTGAAA 
ATTGCCCCTC AATATTATGA CATGAGCAAT TTCCCACAGT GTGAAGCAAA GAGACAGTTG 
GACCGCATTA TTGCCAAACT TCAATCCAAG GAATATTCAC AGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031865.2
CDS	197..2584
Translation	MSKRHRLDLGEDYPSGKKRAGTDGKDRERDRDREDRSKDRDRERDRGDREREREKEKEKELRASTNAMLISAGLPPLKASHSAHSTHSAHSTHSTHSAHSTHTGHTGHTSLPQCINPFTNLPHTPRYYDILKKRLQLPVWEYKDRFTDILVRHQSFVLVGETGSGKTTQIPQWCVEYMRSLPGPKRGVACTQPRRVAAMSVAQRVADEMDVMLGQEVGYSIRFEDCSSAKTILKYMTDGMLLREAMNDPLLERYGVIILDEAHERTLATDILMGVLKEVVRQRSDLKVIVMSATLDAGKFQIYFDNCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHMCEEEEGDLLLFLTGQEEIDEACKRIKREVDDLGPEVGDIKIIPLYSTLPPQQQQRIFEPPPPKKQNGAIGRKVVVSTNIAETSLTIDGVVFVIDPGFAKQKVYNPRIRVESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAYKTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALNDDGDLTELGSMMAEFPLDPQLAKMVIASCDYNCSNEVLSITAMLSVPQCFVRPTEAKKAADEAKMRFAHIDGDHLTLLNVYHAFKQNHESVQWCYDNFINYRSLMSADNVRQQLSRIMDRFNLPRRSTDFTSRDYYINIRKALVTGYFMQVAHLERTGHYLTVKDNQVVQLHPSTVLDHKPEWVLYNEFVLTTKNYIRTCTDIKPEWLVKIAPQYYDMSNFPQCEAKRQLDRIIAKLQSKEYSQY

Target ORF information:

RefSeq Version	NM_007839.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 15 (Dhx15), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007839.3

ORF Insert Sequence:

ATGTCCAAGA GGCATCGGTT GGACCTGGGG GAGGATTACC CCTCCGGCAA GAAGCGTGCG 
GGGACCGATG GGAAAGACCG GGAACGAGAT CGGGATAGAG AGGACCGATC TAAAGATCGT 
GACCGAGAAC GTGATAGAGG AGACAGAGAA CGGGAGAGGG AAAAAGAAAA GGAAAAAGAA 
TTGAGAGCTT CCACCAATGC TATGCTTATC AGTGCTGGAT TGCCACCTTT AAAAGCTTCT 
CATTCAGCTC ACTCAACCCA CTCGGCTCAT TCAACACATT CTACACATTC TGCCCACTCA 
ACACACACTG GACACACAGG ACACACATCA CTTCCACAGT GCATTAATCC ATTCACCAAC 
CTGCCCCATA CTCCTCGATA CTATGATATT CTAAAGAAAC GACTTCAGCT CCCTGTATGG 
GAATACAAGG ATAGATTTAC AGATATTCTT GTTAGACATC AGTCATTTGT ACTTGTTGGT 
GAGACTGGGT CTGGTAAAAC AACACAGATA CCACAGTGGT GTGTGGAGTA TATGCGATCC 
TTGCCAGGAC CCAAAAGAGG AGTCGCCTGT ACACAGCCCA GGAGAGTGGC TGCAATGAGT 
GTGGCTCAGA GAGTTGCTGA TGAGATGGAC GTGATGTTAG GCCAGGAAGT TGGATACTCC 
ATTCGATTTG AAGACTGCAG TAGTGCAAAA ACCATTCTTA AGTATATGAC TGACGGGATG 
CTACTTCGTG AAGCCATGAA CGATCCCCTT CTGGAGCGTT ATGGTGTGAT AATTCTTGAT 
GAGGCCCATG AAAGAACATT GGCTACAGAT ATTCTCATGG GTGTTCTAAA GGAAGTTGTA 
AGACAGAGAT CAGATTTAAA GGTTATAGTT ATGAGTGCTA CCCTAGATGC TGGGAAATTC 
CAGATTTACT TTGATAACTG TCCTCTCTTA ACTATTCCTG GCCGAACACA TCCTGTTGAG 
ATTTTTTATA CTCCAGAACC AGAGAGAGAT TATCTTGAAG CAGCAATTCG GACAGTGATC 
CAAATACATA TGTGTGAAGA GGAGGAAGGA GACCTTCTTC TTTTCTTGAC TGGTCAAGAG 
GAAATTGATG AGGCCTGTAA GAGAATAAAG CGTGAAGTTG ATGATTTGGG CCCTGAAGTT 
GGTGATATTA AAATCATTCC ATTATATTCT ACACTTCCAC CCCAGCAGCA ACAACGCATT 
TTTGAGCCAC CACCTCCAAA AAAACAGAAT GGAGCAATTG GAAGAAAGGT GGTGGTGTCA 
ACTAATATTG CAGAGACCTC TTTGACAATA GACGGTGTGG TGTTTGTAAT TGACCCTGGA 
TTTGCAAAAC AAAAGGTATA TAATCCTAGA ATCAGAGTCG AGTCTCTTCT GGTGACTGCC 
ATTAGTAAAG CTTCAGCTCA GCAAAGGGCG GGTCGAGCTG GACGGACCAG ACCTGGGAAA 
TGCTTCAGGC TTTATACAGA GAAAGCTTAT AAAACAGAAA TGCAGGACAA CACCTATCCT 
GAGATCCTGC GCTCTAACCT AGGATCGGTT GTGTTGCAGC TGAAGAAGCT TGGTATTGAT 
GACTTGGTAC ACTTTGATTT TATGGACCCA CCAGCTCCTG AAACTCTGAT GAGAGCCCTA 
GAACTTTTGA ATTATTTGGC TGCTTTAAAT GATGATGGAG ATCTGACTGA ATTGGGATCC 
ATGATGGCAG AGTTCCCTTT AGATCCACAG CTCGCTAAAA TGGTTATTGC AAGTTGTGAC 
TACAACTGTT CTAATGAGGT CCTATCTATT ACTGCTATGT TGTCAGTCCC ACAGTGTTTT 
GTTCGCCCCA CGGAGGCCAA GAAAGCTGCA GATGAGGCCA AGATGAGATT TGCCCACATA 
GATGGAGATC ATCTGACACT GCTGAATGTC TACCACGCTT TTAAACAAAA TCATGAATCT 
GTTCAGTGGT GTTATGACAA CTTCATTAAC TACCGGTCCC TGATGTCTGC AGACAATGTA 
CGCCAGCAGC TATCAAGAAT TATGGACAGA TTTAATTTGC CTCGTCGAAG TACTGATTTC 
ACAAGCAGGG ACTATTATAT TAATATAAGA AAAGCTTTGG TTACTGGGTA TTTTATGCAA 
GTGGCACATT TAGAACGAAC GGGGCATTAT TTAACTGTGA AAGATAACCA GGTGGTCCAG 
TTGCATCCCT CTACTGTGCT TGATCACAAG CCTGAATGGG TGCTTTATAA TGAGTTTGTG 
CTGACGACAA AGAATTATAT CCGGACATGT ACAGATATCA AGCCAGAATG GTTGGTGAAA 
ATTGCCCCTC AATATTATGA CATGAGCAAT TTCCCACAGT GTGAAGCAAA GAGACAGTTG 
GACCGCATTA TTGCCAAACT TCAATCCAAG GAATATTCAC AGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Conserved expression of ultra-conserved noncoding RNA in mammalian nervous system.
Biochimica et biophysica acta1860(12)1159-1168(2017 Dec)
Zhou J,Wang R,Zhang J,Zhu L,Liu W,Lu S,Chen P,Li H,Yin B,Yuan J,Qiang B,Shu P,Peng X

Nlrp6 regulates intestinal antiviral innate immunity.
Science (New York, N.Y.)350(6262)826-30(2015 Nov)
Wang P,Zhu S,Yang L,Cui S,Pan W,Jackson R,Zheng Y,Rongvaux A,Sun Q,Yang G,Gao S,Lin R,You F,Flavell R,Fikrig E

The Regulatory Factor ZFHX3 Modifies Circadian Function in SCN via an AT Motif-Driven Axis.
Cell162(3)607-21(2015 Jul)
Parsons MJ,Brancaccio M,Sethi S,Maywood ES,Satija R,Edwards JK,Jagannath A,Couch Y,Finelli MJ,Smyllie NJ,Esapa C,Butler R,Barnard AR,Chesham JE,Saito S,Joynson G,Wells S,Foster RG,Oliver PL,Simon MM,Mallon AM,Hastings MH,Nolan PM

DHX15 senses double-stranded RNA in myeloid dendritic cells.
Journal of immunology (Baltimore, Md. : 1950)193(3)1364-72(2014 Aug)
Lu H,Lu N,Weng L,Yuan B,Liu YJ,Zhang Z

Raftlin is involved in the nucleocapture complex to induce poly(I:C)-mediated TLR3 activation.
The Journal of biological chemistry286(12)10702-11(2011 Mar)
Watanabe A,Tatematsu M,Saeki K,Shibata S,Shime H,Yoshimura A,Obuse C,Seya T,Matsumoto M

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Dhx15 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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