lrrk1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following lrrk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lrrk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa55223 XM_012959579.3
Latest version!
Xenopus tropicalis leucine-rich repeat kinase 1 (lrrk1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa30976 XM_012959579.2
Latest version!
Xenopus tropicalis leucine-rich repeat kinase 1 (lrrk1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa55222 XM_012959577.3
Latest version!
Xenopus tropicalis leucine-rich repeat kinase 1 (lrrk1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa55222 XM_012959578.3
Latest version!
Xenopus tropicalis leucine-rich repeat kinase 1 (lrrk1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa30975 XM_012959577.2
Latest version!
Xenopus tropicalis leucine-rich repeat kinase 1 (lrrk1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OXa30975 XM_012959578.2
Latest version!
Xenopus tropicalis leucine-rich repeat kinase 1 (lrrk1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa55223
    Clone ID Related Accession (Same CDS sequence) XM_012959579.3
    Accession Version XM_012959579.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5931bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product leucine-rich repeat serine/threonine-protein kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959579.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGGGAACA AACAAACCCT GCCAGGGGAG TTGCCAGTGA ATCAGAGCCC AGTTATAGCA 
    GACATACAGG CAGCATATTG CGAAGGGGAC AAAGAGAGAG CACAGAATCT AGTGAAAAGT
    GCCTGTGAGG AGAATGTCCA GAAGCATGAG AAGTACCAGT TTCTGAGACT AACAGCAGCC
    TATGGAGATC TTCCCTCTGT ACGTTATCTG CTTAAGGAAG CACAGGTAGT TTTACCAACT
    GAACCAAATG ATGACAATCC AGCAGTAATG GCTTCGCATT TTGGCCATGT TGACACAGTA
    ACATTTTTAC TGGATTCTCT GCCAGATCCC TGCAGTCCCA AGCCACTGCT GAACTGGATG
    CTGGTAACTG CCTGTGAGCA GGGTCACTTG GCTCTGGTTA AACTTTTGGT ACTAAGATAC
    AATGCTGACC CAGACTGTTA TGCTACAAGA AACAATGAGT TCCCTGTCCT CGGGAAATTA
    CCCCTTTATG CCACCATTAT GGCAGGACAT GAAGAAGTTG CTGCCTTTCT GCTTCAAAAT
    GGAGCTGGCT TTTGTTCGTA CATACTGATG GACAACCCAG AGCTGAGCAA AGTTTTACTG
    AGAAGAAGTC TTACCACTGA GCCACTAGAA CACAGTCAGC AGCAAGTAGC ACAGGAGATG
    GCTGTTTGTG TGAAGTGGTC TAGCCTTCGG CTGCCCTGGG TAGACATTGA CTGGTTTATT
    GATATTTCCA ACAAGATAAG AGAGCTGGAT ATCTCTTCCA ACTGCCTTGC CTCCCTTCCA
    TCTGTGGTTC CATGGGGCCT TCTGAACCTG CAGAAACTGA ACATAAGTGA CAATCAGCTT
    TGTGACCTGC CCAGTGTACA GAGCTCTGAG GAAATCATCT GCTCAAAGTT ACTTGAGGTT
    GATGTCTCCA ATAATAAGCT GACATCACTT CCTTCTGCAT TCCTCCACCT GAGCCGGCTC
    CAGAGACTGT GTGCATCAAA AAACTATATT GTTGATCTCT TCAAAGAAGA TGGTACCAAC
    TGGATTGGTT TACGGAGGTT ACAGGATCTA GACTTGCCTG ATAATAGGAT CACAGAACTG
    CCAGCCTCCA TCCTGCACTG CTTCAAATCA CTCAGGAATC TAAATGTAGC TGGAAACAAG
    CTGAAGGTGT TACCTGAACC CTGGTCATGT CCACTTAAAT ATTGCAAGGC TTCAAAAAAT
    GCCATTGAGG GTCTCCCAGA GAACCTGGCT CTTTTCTGGA GGAATCATTT GGTTGAAGTT
    GATTTTTCTG AAAATGCCAT AAAGGAAGTT CCAAAGTCTC TCTTCCAGTT GGAGTGCTTG
    CAGTCTCTGA AGCTCTCCGG GAATCAACTG ACCACACTGC CTTCTTCTGA CACCTGGAAC
    TGCACAAATC TCAAGTGTCT AGATCTGTCC AAGAACCAGC TGGGAAGAAA TGAGGAAGGC
    ACTAGGACCA GACGTATGCC ATTTTTTTAC ACTAAGAGCA GAACAAAGAC AGGGACAGAT
    CCAGGTTCAG CAGTGGAATT TCCATTGTTT CTGAAGGAGT CACTGGAAGT TCTCTACCTA
    AATGACAACC AGTTGGAGTC TGTACCTCCA TCAGTCTCTC AACTAAAGGG CCTTACAGAG
    CTCTACTTGG GAAACAATCC AGGTATACGA GAGCTTCCTG CAGATTTGGG GCAGTTGTCT
    AGTCTGTGGC AACTGGACCT TGAGGATCTG AACATCACAA ACCTGCCCGG GGAGATCAGA
    AAGGAGGGGC CAGTTACTAT CCTGGCTTAC CTGCGTGCTC AGCTGAGAAG GGCAGAACGA
    TGCCATCTGG TTAAAATGAT AATTGTGGGA CCCCCTCGGC AGGGCAAATC TACCCTTTTT
    GAGGTTCTGC AGAGTGGGAA AGTGTCTCAG CAAATGCAAG GGGACAACTC CATAAGAACC
    ACCTGCTGGG AGCTACAGAA GCCTGTGGGG ACCACTGCAA AGGTTGATTC TGTGCAGTTC
    CATGTGTGGG ACGTTGGAGG TTCGAATGCA ATGTCCGCTG TGAACCAGTG CTTCTTCACT
    GACAAAGCCT TGTATGTGGT AGTCTGGAAC CTTGCTCTTG GCGAGGAGGC TGTTGCCAAT
    CTTCAGTTCT GGCTACTGAA CATAGAGGCA AAAGCTCCCA ATGCCGTTGT TCTGGTTGTA
    GGGACGCACC TTGACTTCAT AGAAACCAAA TTCCGGTTGG AGAGAATTGC CACTCTTCGA
    GCTTATGTTC TGGCATTGTG TCGCTCCCCA TCTGGCTCCA GAGCAACTGG GTTCCCTGAT
    ATCACTTTCA AGCACTTACA TGAACTCTCT TGCAAAACAT TGGAGGGACT CGATGGTCTG
    CGCCAGCTGA TATTCCATGC TGCCTGCAAT ATGAGGGATG TAGGCAGTAC CATTGGATCC
    CAAAAACTAG TGGGAAGGCT GATCCCGAGA AGCTACCTGA ACCTTCAGGA GGCTGTGGTA
    GCTGAGCAGC AGAGACGAGC TCAAGGGGAT GAGGTACAAT ACCTGACTGA TGAGCAGATA
    GAACAGATTA TTGAACAGAG CCCTGGAAAC GATATACGCG ATTATGAAGA CATGCAGGCA
    GCGATCGGTT TCTTGATAGA AACTGGGACT TTGCTTCACT TTCCTGACAC TAGCCATGGC
    CTAAGAAACC TTTACTTCTT GGACCCTGTT TGGTTGTCAG AATGTTTGGA GAGGATTATT
    AACATTAAGA GCTCCAAGTC TGTGGCCCGG AATGGGGTGA TTAAGGCAGA AGATCTCAGA
    ATGCTGCTGG TTGGAACTGG ATTCACCAAA CAGACTGAAG AGCAATATTT TCAGTTCCTG
    GCAAAGTTTG AGATTGCTCT TCCTGTTGCC AATAACAGTT ACCTTTTGCC ACATTTGCTG
    CCGACAAAGC CCGGCCTGGA CATCCACAGC TTGCGGCAGC TTACTGGAAG CACAGTTCAG
    AGGTTGTTTA AGATGAGCTT TGTTCCTGTT GGGTTCTGGC AGCGGTTCAT AGCTCGGATG
    TTGATCAGTC TGGCAGAAAT GGATCTCCAG CTGTTTGAAA GCAAGAAAAA CACCAAGACA
    AAAAACAGGA AATCTGCAAT TTACAGCTTC ACTGGGTATC AGAGGAACCG ATGTAGCACC
    TTCCGAGTGA AGAGGAATCA GACCATTTAC TGGCAGGAAG GCCTAATGGT CACCTTTGAA
    GGGGGTTACC TCAGTGTTGA GTCATCAGAC ATTAACTGGA AGAAGAAGAA GAATGGTGGG
    ATTAAAATAA TTTGCCAGTC TGAGCTGAGA GATTTCTCTG CTATGGCATT TATCACAGAC
    CATGTGAACT CTCTCATAGA TCAGTGGTTC CCAGCCCTTA CAGCCACAGA GAGTGATGGC
    ACTTTACTGA TGGAGCAATG TGTGCCCTGC CACTGCTGTA CCACAGTCGC TAGTGATCAA
    GAGAAGAAAA ATAATGAGAA TACCCCACAT TACTTTAATA TGGAAGACTG CGTCCTAGCA
    GCTGTTGAGA AGGATTATTT GGAATGCCCG AACCACCCAG AACATGCAGT GCCACTGCAG
    GAACTGGTCC CAGAAATTTT CATGACTGAC TTTCCTTCAA GGCTTTTCCT TGACATCAAT
    GACTTAGAAT ACTCACAGGA GAGCTCCAAC ATTCTGGGTC AGGGAGGAAG TGGCACAGTG
    ATATACCGAG CTGAATATAA GCACAAGCCT GTGGCTGTGA AGATGTTTCA GGTTTCTAAG
    TGTCGGGCAT CTCCAGAGGT CATGCAAGAC ACCATGTTCA AGCACTTGCA GGCAATGGAC
    GCCATGAGAA ACTTCTCGGA ATTCCGTCAG GAGGCCAGCA TGCTCCATAC TCTGCAGCAC
    CCCTGCATTG TCTCCCTGAT TGGCATCAGC ATCCATCCAC TGTGCTTTGC CCTGGAGCTG
    GCACCTTTGG GGAGCCTCAA CACAGTGCTG GCAGGAACAG CCCAAGATGC AAGTTCATTC
    ATGCCTCTTG GTCACATGCT CACTCAGCGT ATTTCCTATC AAATTGCATC TGGGCTGTCC
    TACCTGCATA AAAAGAATAT AATATTTTGT GACCTGAAAT CAGATAACAT CCTGGTTTGG
    TCCCTGGATG TCAAGGAAGC TGTGATAATC AAACTCTCAG ACTATGGCAT TTCGCGGCTG
    TCATTCCATG AGGGAGCCCT GGGTGTGGAA GGCACCCCAG GATATCAAGC ACCCGAAATC
    CGTCCTCGGA TTGTGTATGA TGAAAAGGTG GATATGTTTT CATATGGTAT GGTCCTGTAT
    GAGCTATTGT CAGGCCAGAG ACCATCTTTG GGCCAGCACC AGCTGCAGAT TTCCAAGAAA
    CTGTCCAAGG GGATTCGTCC AGTTCTTGGA CGCCCAGAGG AAGTTCACTT CCATCATCTG
    CAGGACCTGA TGATGGAATG TTGGGACACG AAGCCTGAAA AGCGGCCTTT AGCTTCATCA
    GTTGTCCATC GTATGAATGA CCCCAGCTTC ACAGTTTTTA AGTACCAGTT GTCTTGTGGG
    ATACAGGAAT CATTCTGCTC ATCCAGAGAA CCAGAGAGCA CAGTTGTATT CTGGGTAGGA
    ACAGGGACAT CAAGGAATTA TACCGTGGTT GATGTTGAGA AAGGGCAGGT GGAAGTGCAG
    ACTCTGAGCT GCCCCGGGAT GAGAATGTCT TGCCAGCAAA GGGTACAGAA TACACTTTGG
    ATTGCAACAG AGGATCAGAA GATATCTATC TTTAGTCTGC AGGGCATGTG CCCCCTAAGT
    GTCGCACTAA AGGTGATAGA CACCCCTGCC ATTGTGACCT GCTTCCTTGT AGCGAATATG
    GAAAAGGGGA ACTGCCTGGT ATTTGCTGGC CTTTCAGACA GCCTGATTTC AGTGTTCCCA
    GTAAGTGGTG GCATCCCAGG CAGTGATTGC TCCTACATCT GTTCCCGCAC AGCTAATCGC
    TCCATGTTCA AGATAACAGA TGATGACCCA CGTCAGAAAC CTCACCCAGT GTTGGCCATG
    GAGATCTGCA ATAAATGTTC TGAGGTCTGG TATGGCAATG GACCTGGTGT CCTTGTAGTG
    GACTGTACCT CTCTTGAGAT TTCAAAAAGA CTGGAACCCT TTAGTGCACC ATCATCCATC
    ATCTCCATGG TTACAAGCAC AGACACTAAC AGAGAGGAGA CTGTTTGGTT ATTGGATGAT
    ATGACCAATT TTCTGGTCAT GTATAATGCT GCAGCCTACC AACTCTGTGC CCGATACTTC
    TGTGGTGATA GCAGTCCTCT AAGGGATATG TTTACAGTAA AGCCTTGTTC AGATGTGGTT
    CCTGTTGCAT TGCCAGTTAC AAACTGTAAT AGCCCCCGCT CAGCAAACGT CAGCATCATC
    TACAGCCTAG AACGTGGTAT GCAAGTTCTC AGCCACCAAG ACTCTCTTAC CGATTATTGC
    TCTGTGTCAT CCTTCTATTC TTCCATTCCT CAGGGGACTG CTAAGTCCTC CTCCAGTTTG
    CCAAGCACTC CGGTCAGCTA CAGTAGTGCC CCATTTTCCA CAGATAGTGA GGATCCAGAC
    CCAGCCCAAG AACTTATACA CAAAATTTCT AGTGAGAGCT GGGGGGGCAG AACACCTCTT
    TCAGAGGAGG AAAAACTCAA TGTGCAGGCT GTTGGGATTG TAGTCGTCAA AGATTTGCTC
    TGGGTTCCGA GGCGCGGAGG GGACATTATT ATCATTGGAC TGGAGAAGGA GTGTGGTGGA
    CAAAGAGGTC GTGTCATTGC TGTGTTACAA ACTGGCCTAG TAGAAAATCA AGGGGTCCAT
    CTCCACGCTG CTGCTCTTTG CAAGGATTCG GTTGTGTGCT GCTTGCAGAG TGAGACACTA
    GGGTGCTGCT TGTCCGTCTG GAGGAATTGG GGATCAGAGG AGTTTAACAT TTTTTATCAT
    GCCTCTGATG AACTTAGAAG GCTGGAAAAC TGCATGAGGA AAAGGAGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815033.2
    CDS156..6086
    Translation

    Target ORF information:

    RefSeq Version XM_012959579.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis leucine-rich repeat kinase 1 (lrrk1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959579.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGGGAACA AACAAACCCT GCCAGGGGAG TTGCCAGTGA ATCAGAGCCC AGTTATAGCA 
    GACATACAGG CAGCATATTG CGAAGGGGAC AAAGAGAGAG CACAGAATCT AGTGAAAAGT
    GCCTGTGAGG AGAATGTCCA GAAGCATGAG AAGTACCAGT TTCTGAGACT AACAGCAGCC
    TATGGAGATC TTCCCTCTGT ACGTTATCTG CTTAAGGAAG CACAGGTAGT TTTACCAACT
    GAACCAAATG ATGACAATCC AGCAGTAATG GCTTCGCATT TTGGCCATGT TGACACAGTA
    ACATTTTTAC TGGATTCTCT GCCAGATCCC TGCAGTCCCA AGCCACTGCT GAACTGGATG
    CTGGTAACTG CCTGTGAGCA GGGTCACTTG GCTCTGGTTA AACTTTTGGT ACTAAGATAC
    AATGCTGACC CAGACTGTTA TGCTACAAGA AACAATGAGT TCCCTGTCCT CGGGAAATTA
    CCCCTTTATG CCACCATTAT GGCAGGACAT GAAGAAGTTG CTGCCTTTCT GCTTCAAAAT
    GGAGCTGGCT TTTGTTCGTA CATACTGATG GACAACCCAG AGCTGAGCAA AGTTTTACTG
    AGAAGAAGTC TTACCACTGA GCCACTAGAA CACAGTCAGC AGCAAGTAGC ACAGGAGATG
    GCTGTTTGTG TGAAGTGGTC TAGCCTTCGG CTGCCCTGGG TAGACATTGA CTGGTTTATT
    GATATTTCCA ACAAGATAAG AGAGCTGGAT ATCTCTTCCA ACTGCCTTGC CTCCCTTCCA
    TCTGTGGTTC CATGGGGCCT TCTGAACCTG CAGAAACTGA ACATAAGTGA CAATCAGCTT
    TGTGACCTGC CCAGTGTACA GAGCTCTGAG GAAATCATCT GCTCAAAGTT ACTTGAGGTT
    GATGTCTCCA ATAATAAGCT GACATCACTT CCTTCTGCAT TCCTCCACCT GAGCCGGCTC
    CAGAGACTGT GTGCATCAAA AAACTATATT GTTGATCTCT TCAAAGAAGA TGGTACCAAC
    TGGATTGGTT TACGGAGGTT ACAGGATCTA GACTTGCCTG ATAATAGGAT CACAGAACTG
    CCAGCCTCCA TCCTGCACTG CTTCAAATCA CTCAGGAATC TAAATGTAGC TGGAAACAAG
    CTGAAGGTGT TACCTGAACC CTGGTCATGT CCACTTAAAT ATTGCAAGGC TTCAAAAAAT
    GCCATTGAGG GTCTCCCAGA GAACCTGGCT CTTTTCTGGA GGAATCATTT GGTTGAAGTT
    GATTTTTCTG AAAATGCCAT AAAGGAAGTT CCAAAGTCTC TCTTCCAGTT GGAGTGCTTG
    CAGTCTCTGA AGCTCTCCGG GAATCAACTG ACCACACTGC CTTCTTCTGA CACCTGGAAC
    TGCACAAATC TCAAGTGTCT AGATCTGTCC AAGAACCAGC TGGGAAGAAA TGAGGAAGGC
    ACTAGGACCA GACGTATGCC ATTTTTTTAC ACTAAGAGCA GAACAAAGAC AGGGACAGAT
    CCAGGTTCAG CAGTGGAATT TCCATTGTTT CTGAAGGAGT CACTGGAAGT TCTCTACCTA
    AATGACAACC AGTTGGAGTC TGTACCTCCA TCAGTCTCTC AACTAAAGGG CCTTACAGAG
    CTCTACTTGG GAAACAATCC AGGTATACGA GAGCTTCCTG CAGATTTGGG GCAGTTGTCT
    AGTCTGTGGC AACTGGACCT TGAGGATCTG AACATCACAA ACCTGCCCGG GGAGATCAGA
    AAGGAGGGGC CAGTTACTAT CCTGGCTTAC CTGCGTGCTC AGCTGAGAAG GGCAGAACGA
    TGCCATCTGG TTAAAATGAT AATTGTGGGA CCCCCTCGGC AGGGCAAATC TACCCTTTTT
    GAGGTTCTGC AGAGTGGGAA AGTGTCTCAG CAAATGCAAG GGGACAACTC CATAAGAACC
    ACCTGCTGGG AGCTACAGAA GCCTGTGGGG ACCACTGCAA AGGTTGATTC TGTGCAGTTC
    CATGTGTGGG ACGTTGGAGG TTCGAATGCA ATGTCCGCTG TGAACCAGTG CTTCTTCACT
    GACAAAGCCT TGTATGTGGT AGTCTGGAAC CTTGCTCTTG GCGAGGAGGC TGTTGCCAAT
    CTTCAGTTCT GGCTACTGAA CATAGAGGCA AAAGCTCCCA ATGCCGTTGT TCTGGTTGTA
    GGGACGCACC TTGACTTCAT AGAAACCAAA TTCCGGTTGG AGAGAATTGC CACTCTTCGA
    GCTTATGTTC TGGCATTGTG TCGCTCCCCA TCTGGCTCCA GAGCAACTGG GTTCCCTGAT
    ATCACTTTCA AGCACTTACA TGAACTCTCT TGCAAAACAT TGGAGGGACT CGATGGTCTG
    CGCCAGCTGA TATTCCATGC TGCCTGCAAT ATGAGGGATG TAGGCAGTAC CATTGGATCC
    CAAAAACTAG TGGGAAGGCT GATCCCGAGA AGCTACCTGA ACCTTCAGGA GGCTGTGGTA
    GCTGAGCAGC AGAGACGAGC TCAAGGGGAT GAGGTACAAT ACCTGACTGA TGAGCAGATA
    GAACAGATTA TTGAACAGAG CCCTGGAAAC GATATACGCG ATTATGAAGA CATGCAGGCA
    GCGATCGGTT TCTTGATAGA AACTGGGACT TTGCTTCACT TTCCTGACAC TAGCCATGGC
    CTAAGAAACC TTTACTTCTT GGACCCTGTT TGGTTGTCAG AATGTTTGGA GAGGATTATT
    AACATTAAGA GCTCCAAGTC TGTGGCCCGG AATGGGGTGA TTAAGGCAGA AGATCTCAGA
    ATGCTGCTGG TTGGAACTGG ATTCACCAAA CAGACTGAAG AGCAATATTT TCAGTTCCTG
    GCAAAGTTTG AGATTGCTCT TCCTGTTGCC AATAACAGTT ACCTTTTGCC ACATTTGCTG
    CCGACAAAGC CCGGCCTGGA CATCCACAGC TTGCGGCAGC TTACTGGAAG CACAGTTCAG
    AGGTTGTTTA AGATGAGCTT TGTTCCTGTT GGGTTCTGGC AGCGGTTCAT AGCTCGGATG
    TTGATCAGTC TGGCAGAAAT GGATCTCCAG CTGTTTGAAA GCAAGAAAAA CACCAAGACA
    AAAAACAGGA AATCTGCAAT TTACAGCTTC ACTGGGTATC AGAGGAACCG ATGTAGCACC
    TTCCGAGTGA AGAGGAATCA GACCATTTAC TGGCAGGAAG GCCTAATGGT CACCTTTGAA
    GGGGGTTACC TCAGTGTTGA GTCATCAGAC ATTAACTGGA AGAAGAAGAA GAATGGTGGG
    ATTAAAATAA TTTGCCAGTC TGAGCTGAGA GATTTCTCTG CTATGGCATT TATCACAGAC
    CATGTGAACT CTCTCATAGA TCAGTGGTTC CCAGCCCTTA CAGCCACAGA GAGTGATGGC
    ACTTTACTGA TGGAGCAATG TGTGCCCTGC CACTGCTGTA CCACAGTCGC TAGTGATCAA
    GAGAAGAAAA ATAATGAGAA TACCCCACAT TACTTTAATA TGGAAGACTG CGTCCTAGCA
    GCTGTTGAGA AGGATTATTT GGAATGCCCG AACCACCCAG AACATGCAGT GCCACTGCAG
    GAACTGGTCC CAGAAATTTT CATGACTGAC TTTCCTTCAA GGCTTTTCCT TGACATCAAT
    GACTTAGAAT ACTCACAGGA GAGCTCCAAC ATTCTGGGTC AGGGAGGAAG TGGCACAGTG
    ATATACCGAG CTGAATATAA GCACAAGCCT GTGGCTGTGA AGATGTTTCA GGTTTCTAAG
    TGTCGGGCAT CTCCAGAGGT CATGCAAGAC ACCATGTTCA AGCACTTGCA GGCAATGGAC
    GCCATGAGAA ACTTCTCGGA ATTCCGTCAG GAGGCCAGCA TGCTCCATAC TCTGCAGCAC
    CCCTGCATTG TCTCCCTGAT TGGCATCAGC ATCCATCCAC TGTGCTTTGC CCTGGAGCTG
    GCACCTTTGG GGAGCCTCAA CACAGTGCTG GCAGGAACAG CCCAAGATGC AAGTTCATTC
    ATGCCTCTTG GTCACATGCT CACTCAGCGT ATTTCCTATC AAATTGCATC TGGGCTGTCC
    TACCTGCATA AAAAGAATAT AATATTTTGT GACCTGAAAT CAGATAACAT CCTGGTTTGG
    TCCCTGGATG TCAAGGAAGC TGTGATAATC AAACTCTCAG ACTATGGCAT TTCGCGGCTG
    TCATTCCATG AGGGAGCCCT GGGTGTGGAA GGCACCCCAG GATATCAAGC ACCCGAAATC
    CGTCCTCGGA TTGTGTATGA TGAAAAGGTG GATATGTTTT CATATGGTAT GGTCCTGTAT
    GAGCTATTGT CAGGCCAGAG ACCATCTTTG GGCCAGCACC AGCTGCAGAT TTCCAAGAAA
    CTGTCCAAGG GGATTCGTCC AGTTCTTGGA CGCCCAGAGG AAGTTCACTT CCATCATCTG
    CAGGACCTGA TGATGGAATG TTGGGACACG AAGCCTGAAA AGCGGCCTTT AGCTTCATCA
    GTTGTCCATC GTATGAATGA CCCCAGCTTC ACAGTTTTTA AGTACCAGTT GTCTTGTGGG
    ATACAGGAAT CATTCTGCTC ATCCAGAGAA CCAGAGAGCA CAGTTGTATT CTGGGTAGGA
    ACAGGGACAT CAAGGAATTA TACCGTGGTT GATGTTGAGA AAGGGCAGGT GGAAGTGCAG
    ACTCTGAGCT GCCCCGGGAT GAGAATGTCT TGCCAGCAAA GGGTACAGAA TACACTTTGG
    ATTGCAACAG AGGATCAGAA GATATCTATC TTTAGTCTGC AGGGCATGTG CCCCCTAAGT
    GTCGCACTAA AGGTGATAGA CACCCCTGCC ATTGTGACCT GCTTCCTTGT AGCGAATATG
    GAAAAGGGGA ACTGCCTGGT ATTTGCTGGC CTTTCAGACA GCCTGATTTC AGTGTTCCCA
    GTAAGTGGTG GCATCCCAGG CAGTGATTGC TCCTACATCT GTTCCCGCAC AGCTAATCGC
    TCCATGTTCA AGATAACAGA TGATGACCCA CGTCAGAAAC CTCACCCAGT GTTGGCCATG
    GAGATCTGCA ATAAATGTTC TGAGGTCTGG TATGGCAATG GACCTGGTGT CCTTGTAGTG
    GACTGTACCT CTCTTGAGAT TTCAAAAAGA CTGGAACCCT TTAGTGCACC ATCATCCATC
    ATCTCCATGG TTACAAGCAC AGACACTAAC AGAGAGGAGA CTGTTTGGTT ATTGGATGAT
    ATGACCAATT TTCTGGTCAT GTATAATGCT GCAGCCTACC AACTCTGTGC CCGATACTTC
    TGTGGTGATA GCAGTCCTCT AAGGGATATG TTTACAGTAA AGCCTTGTTC AGATGTGGTT
    CCTGTTGCAT TGCCAGTTAC AAACTGTAAT AGCCCCCGCT CAGCAAACGT CAGCATCATC
    TACAGCCTAG AACGTGGTAT GCAAGTTCTC AGCCACCAAG ACTCTCTTAC CGATTATTGC
    TCTGTGTCAT CCTTCTATTC TTCCATTCCT CAGGGGACTG CTAAGTCCTC CTCCAGTTTG
    CCAAGCACTC CGGTCAGCTA CAGTAGTGCC CCATTTTCCA CAGATAGTGA GGATCCAGAC
    CCAGCCCAAG AACTTATACA CAAAATTTCT AGTGAGAGCT GGGGGGGCAG AACACCTCTT
    TCAGAGGAGG AAAAACTCAA TGTGCAGGCT GTTGGGATTG TAGTCGTCAA AGATTTGCTC
    TGGGTTCCGA GGCGCGGAGG GGACATTATT ATCATTGGAC TGGAGAAGGA GTGTGGTGGA
    CAAAGAGGTC GTGTCATTGC TGTGTTACAA ACTGGCCTAG TAGAAAATCA AGGGGTCCAT
    CTCCACGCTG CTGCTCTTTG CAAGGATTCG GTTGTGTGCT GCTTGCAGAG TGAGACACTA
    GGGTGCTGCT TGTCCGTCTG GAGGAATTGG GGATCAGAGG AGTTTAACAT TTTTTATCAT
    GCCTCTGATG AACTTAGAAG GCTGGAAAAC TGCATGAGGA AAAGGAGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30976
    Clone ID Related Accession (Same CDS sequence) XM_012959579.2
    Accession Version XM_012959579.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5931bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product leucine-rich repeat serine/threonine-protein kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959579.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGGGAACA AACAAACCCT GCCAGGGGAG TTGCCAGTGA ATCAGAGCCC AGTTATAGCA 
    GACATACAGG CAGCATATTG CGAAGGGGAC AAAGAGAGAG CACAGAATCT AGTGAAAAGT
    GCCTGTGAGG AGAATGTCCA GAAGCATGAG AAGTACCAGT TTCTGAGACT AACAGCAGCC
    TATGGAGATC TTCCCTCTGT ACGTTATCTG CTTAAGGAAG CACAGGTAGT TTTACCAACT
    GAACCAAATG ATGACAATCC AGCAGTAATG GCTTCGCATT TTGGCCATGT TGACACAGTA
    ACATTTTTAC TGGATTCTCT GCCAGATCCC TGCAGTCCCA AGCCACTGCT GAACTGGATG
    CTGGTAACTG CCTGTGAGCA GGGTCACTTG GCTCTGGTTA AACTTTTGGT ACTAAGATAC
    AATGCTGACC CAGACTGTTA TGCTACAAGA AACAATGAGT TCCCTGTCCT CGGGAAATTA
    CCCCTTTATG CCACCATTAT GGCAGGACAT GAAGAAGTTG CTGCCTTTCT GCTTCAAAAT
    GGAGCTGGCT TCTGTTCGTA CATACTGATG GACAACCCAG AGCTGAGCAA AGTTTTACTG
    AGAAGAAGTC TTACCACTGA GCCACTAGAA CACAGTCAGC AGCAAGTAGC ACAGGAGATG
    GCTGTTTGTG TGAAGTGGTC TAGCCTTCGG CTGCCCTGGG TAGACATTGA CTGGTTTATT
    GATATTTCCA ACAAGATAAG AGAGCTGGAT ATCTCTTCCA ACTGCCTTGC CTCCCTTCCA
    TCTGTGGTTC CATGGGGCCT TCTGAACCTG CAGAAACTGA ACATAAGTGA CAATCAGCTT
    TGTGACCTGC CCAGTGTACA GAGCTCTGAG GAAATCATCT GCTCAAAGTT ACTTGAGGTT
    GATGTCTCCA ATAATAAGCT GACATCACTT CCTTCTGCAT TCCTCCACCT GAGCCGGCTC
    CAGAGACTGT GTGCATCAAA AAACTATATT GTTGATCTCT TCAAAGAAGA TGGTACCAAC
    TGGATTGGTT TACGGAGGTT ACAGGATCTA GACTTGTCTG ATAATAGGAT CACAGAACTG
    CCAGCCTCCA TCCTGCACTG CTTCAAATCA CTCAGGAATC TAAATGTAGC TGGAAACAAG
    CTGAAGGTGT TACCTGAACC CTGGTCATGT CCACTTAAAT ATTGCAAGGC TTCAAAAAAT
    GCCATTGAGG GTCTCCCAGA GAACCTGGCT CTTTTCTGGA GGAATCATTT GGTTGAAGTT
    GATTTTTCTG AAAATGCCAT AAAGGAAGTT CCAAAGTCTC TCTTCCAGTT GGAGTGCTTG
    CAGTCTCTGA AGCTCTCCGG GAATCAACTG ACCACACTGC CTTCTTCTGA CACCTGGAAC
    TGCACAAATC TCAAGTGTCT AGATCTGTCC AAGAACCAGC TGGGAAGAAA TGAGGAAGGC
    ACTAGGACCA GACGTATGCC ATTTTTTTAC ACTAAGAGCA GAACAAAGAC AGGGACAGAT
    CCAGGTTCAG CAGTGGAATT TCCATTGTTT CTGAAGGAGT CACTGGAAGT TCTCTACCTA
    AATGACAACC AGTTGGAGTC TGTACCTCCA TCAATCTCTC AACTAAAGGG CCTTACAGAG
    CTCTACTTGG GAAACAATCC AGGTATACGA GAGCTTCCTG CAGATTTGGG GCAGTTGTCT
    AGTCTGTGGC AACTGGACCT TGAGGATCTG AACATCACAA ACCTGCCCGG GGAGATCAGA
    AAGGAGGGGC CAGTTACTAT CCTGGCTTAC CTGCGTGCTC AGCTGAGAAG GGCAGAACGA
    TGCCATCTGG TTAAAATGAT AATTGTGGGA CCCCCTCGGC AGGGCAAATC TACCCTTTTT
    GAGGTTCTGC AGAGTGGGAA AGTGTCTCAG CAAATGCAAG GGGACAACTC CATAAGAACC
    ACCTGCTGGG AGCTACAGAA GCCTGTGGGG ACCACTGCAA AGGTTGATTC TGTGCAGTTC
    CATGTGTGGG ACGTTGGAGG TTCGAATGCA ATGTCCGCTG TGAACCAGTG CTTCTTCACT
    GACAAAGCCT TGTATGTGGT AGTCTGGAAC CTTGCTCTTG GCGAGGAGGC TGTTGCCAAT
    CTTCAGTTCT GGCTACTGAA CATAGAGGCA AAAGCTCCCA ATGCCGTTGT TCTGGTTGTA
    GGGACGCACC TTGACTTCAT AGAAACCAAA TTCCGGTTGG AGAGAATTGC CACTCTTCGA
    GCTTATGTTC TGGCATTGTG TCGCTCCCCA TCTGGCTCCA GAGCAACTGG GTTCCCTGAT
    ATCACTTTCA AGCACTTACA TGAACTCTCT TGCAAAACAT TGGAGGGACT CGATGGTCTG
    CGCCAGCTGA TATTCCATGC TGCCTGCAAT ATGAGGGATG TAGGCAGTAC CATTGGATCC
    CAAAAACTAG TGGGAAGGCT GATCCCGAGA AGCTACCTGA ACCTTCAGGA GGCTGTGGTA
    GCTGAGCAGC AGAGACGAGC TCAAGGGGAT GAGGTACAAT ACCTGACTGA TGAGCAGATA
    GAACAGATTA TTGAACAGAG CCCTGGAAAC GATATACGCG ATTATGAAGA CATGCAGGCA
    GCGATCGGTT TCTTGATAGA AACTGGGACT TTGCTTCACT TTCCTGACAC TAGCCATGGC
    CTAAGAAACC TTTACTTCTT GGACCCTGTT TGGTTGTCAG AATGTTTGGA GAGGATTATT
    AACATTAAGA GCTCCAAGTC TGTGGCCCGG AATGGGGTGA TTAAGGCAGA AGATCTCAGA
    ATGCTGCTGG TTGGAACTGG ATTCACCAAA CAGACTGAAG AGCAATATTT TCAGTTCCTG
    GCAAAGTTTG AGATTGCTCT TCCTGTTGCC AATAACAGTT ACCTTTTGCC ACATTTGCTG
    CCGACAAAGC CCGGCCTGGA CATCCACAGC TTGCGGCAGC TTACTGGAAG CACAGTTCAG
    AGGTTGTTTA AGATGAGCTT TGTTCCTGTT GGGTTCTGGC AGCGGTTCAT AGCTCGGATG
    TTGATCAGTC TGGCAGAAAT GGATCTCCAG CTGTTTGAAA GCAAGAAAAA CACCAAGACA
    AAAAACAGGA AATCTGCAAT TTACAGCTTC ACTGGGTATC AGAGGAACCG ATGTAGCACC
    TTCCGAGTGA AGAGGAATCA GACCATTTAC TGGCAGGAAG GCCTAATGGT CACCTTTGAA
    GGGGGTTACC TCAGTGTTGA GTCATCAGAC ATTAACTGGA AGAAGAAGAA GAATGGTGGG
    ATTAAAATAA TTTGCCAGTC TGAGCTGAGA GATTTCTCTG CTATGGCATT TATCACAGAC
    CATGTGAACT CTCTCATAGA TCAGTGGTTC CCAGCCCTTA CAGCCACAGA GAGTGATGGC
    ACTTTACTGA TGGAGCAATG TGTGCCCTGC CACTGCTGTA CCACAGTCGC TAGTGATCAA
    GAGAAGAAAA ATAATGAGAA TACCCCACAT TACTTTAATA TGGAAGACTG CGTCCTAGCA
    GCTGTTGAGA AGGATTATTT GGAATGCCCG AACCACCCAG AACATGCAGT GCCACTGCAG
    GAACTGGTCC CAGAAATTTT CATGACTGAC TTTCCTTCAA GGCTTTTCCT TGACATCAAT
    GACTTAGAAT ACTCACAGGA GAGCTCCAAC ATTCTGGGTC AGGGAGGAAG TGGCACAGTG
    ATATACCGAG CTGAATATAA GCACAAGCCT GTGGCTGTGA AGATGTTTCA GGTTTCTAAG
    TGTCGGGCAT CTCCAGAGGT CATGCAAGAC ACCATGTTCA AGCACTTGCA GGCAATGGAC
    GCCATGAGAA ACTTCTCGGA ATTCCGTCAG GAGGCCAGCA TGCTCCATAC TCTGCAGCAC
    CCCTGCATTG TCTCCCTGAT TGGCATCAGC ATCCATCCAC TGTGCTTTGC CCTGGAGCTG
    GCACCTTTGG GGAGCCTCAA CACAGTGCTG GCAGGAACAG CCCAAGATGC AAGTTCATTC
    ATGCCTCTTG GTCACATGCT CACTCAGCGT ATTTCCTATC AAATTGCATC TGGGCTGTCC
    TACCTGCATA AAAAGAATAT AATATTTTGT GACCTGAAAT CAGATAACAT CCTGGTTTGG
    TCCCTGGATG TCAAGGAAGC TGTGATAATC AAACTCTCAG ACTATGGCAT TTCGCGGCTG
    TCATTCCATG AGGGAGCCCT GGGTGTGGAA GGCACCCCAG GATATCAAGC ACCCGAAATC
    CGTCCTCGGA TTGTGTATGA TGAAAAGGTG GATATGTTTT CATATGGTAT GGTCCTGTAT
    GAGCTATTGT CAGGCCAGAG ACCATCTTTG GGTCAGCACC AGCTGCAGAT TTCCAAGAAA
    CTGTCCAAGG GGATTCGTCC AGTTCTTGGA CGCCCAGAGG AAGTTCACTT CCATCATCTG
    CAGGACCTGA TGATGGAATG TTGGGACACG AAGCCTGAAA AGCGGCCTTT AGCTTCATCA
    GTTGTCCATC GTATGAATGA CCCCAGCTTC ACAGTTTTTA AGTACCAGTT GTCTTGTGGG
    ATACAGGAAT CATTCTGCTC ATCCAGAGAA CCAGAGAGCA CAGTTGTATT CTGGGTAGGA
    ACAGGGACAT CAAGGAATTA TACCGTGGTT GATGTTGAGA AAGGGCAGGT GGAAGTGCAG
    ACTCTGAGCT GCCCCGGGAT GAGAATGTCT TGCCAGCAAA GGGTACAGAA TACACTTTGG
    ATTGCAACAG AGGATCAGAA GATATCTATC TTTAGTCTGC AGGGCATGTG CCCCCTAAGT
    GTCGCACTAA AGGTGATAGA CACCCCTGCC ATTGTGACCT GCTTCCTTGT AGCGAATATG
    GAAAAGGGGA ACTGCCTGGT ATTTGCTGGC CTTTCAGACA GCCTGATTTC AGTGTTCCCA
    GTAAGTGGTG GCATCCCAGG CAGTGATTGC TCCTACATCT GTTCCCGCAC AGCTAATCGC
    TCCATGTTCA AGATAACAGA TGATGACCCA CGTCAGAAAC CTCACCCAGT GTTGGCCATG
    GAGATCTGCA ATAAATGTTC TGAGGTCTGG TATGGCAATG GACCTGGTGT CCTTGTAGTG
    GACTGTACCT CTCTTGAGAT TTCAAAAAGA CTGGAACCCT TTAGTGCACC ATCATCCATC
    ATCTCCATGG TTACAAGCAC AGACACTAAC AGAGAGGAGA CTGTTTGGTT ATTGGATGAT
    ATGACCAATT TTCTGGTCAT GTATAATGCT GCAGCCTACC AACTCTGTGC CCGATACTTC
    TGTGGTGATA GCAGTCCTCT AAGGGATATG TTTACAGTAA AGCCTTGTTC AGATGTGGTT
    CCTGTTGCAT TGCCAGTTAC AAACTGTAAT AGCCCCCGCT CAGCAAACGT CAGCATCATC
    TACAGCCTAG AACGTGGTAT GCAAGTTCTC AGCCACCAAG ACTCTCTTAC CGATTATTGC
    TCTGTGTCAT CCTTCTATTC TTCCATTCCT CAGGGGACTG CTAAGTCCTC CTCCAGTTTG
    CCAAGCACTC CGGTCAGCTA CAGTAGTGCC CCATTTTCCA CAGATAGTGA GGATCCAGAC
    CCAGCCCAAG AACTTATACA CAAAATTTCT AGTGAGAGCT GGGGGGGCAG AACACCTCTT
    TCAGAGGAGG AAAAACTCAA TGTGCAGGCT GTTGGGATTG TAGTCGTCAA AGATTTGCTC
    TGGGTTCCGA GGCGCGGAGG GGACATTATT ATCATTGGAC TGGAGAAGGA GTGTGGTGGA
    CAAAGAGGTC GTGTCATTGC TGTGTTACAA ACTGGCCTAG TAGAAAATCA AGGGGTCCAT
    CTCCACGCTG CTGCTCTTTG CAAGGATTCG GTTGTGTGCT GCTTGCAGAG TGAGACACTA
    GGGTGCTGCT TGTCCGTCTG GAGGAATTGG GGATCAGAGG AGTTTAACAT TTTTTATCAT
    GCCTCTGATG AACTTAGAAG GCTGGAAAAC TGCATGAGGA AAAGGAGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815033.1
    CDS197..6127
    Translation

    Target ORF information:

    RefSeq Version XM_012959579.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis leucine-rich repeat kinase 1 (lrrk1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959579.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGGGAACA AACAAACCCT GCCAGGGGAG TTGCCAGTGA ATCAGAGCCC AGTTATAGCA 
    GACATACAGG CAGCATATTG CGAAGGGGAC AAAGAGAGAG CACAGAATCT AGTGAAAAGT
    GCCTGTGAGG AGAATGTCCA GAAGCATGAG AAGTACCAGT TTCTGAGACT AACAGCAGCC
    TATGGAGATC TTCCCTCTGT ACGTTATCTG CTTAAGGAAG CACAGGTAGT TTTACCAACT
    GAACCAAATG ATGACAATCC AGCAGTAATG GCTTCGCATT TTGGCCATGT TGACACAGTA
    ACATTTTTAC TGGATTCTCT GCCAGATCCC TGCAGTCCCA AGCCACTGCT GAACTGGATG
    CTGGTAACTG CCTGTGAGCA GGGTCACTTG GCTCTGGTTA AACTTTTGGT ACTAAGATAC
    AATGCTGACC CAGACTGTTA TGCTACAAGA AACAATGAGT TCCCTGTCCT CGGGAAATTA
    CCCCTTTATG CCACCATTAT GGCAGGACAT GAAGAAGTTG CTGCCTTTCT GCTTCAAAAT
    GGAGCTGGCT TCTGTTCGTA CATACTGATG GACAACCCAG AGCTGAGCAA AGTTTTACTG
    AGAAGAAGTC TTACCACTGA GCCACTAGAA CACAGTCAGC AGCAAGTAGC ACAGGAGATG
    GCTGTTTGTG TGAAGTGGTC TAGCCTTCGG CTGCCCTGGG TAGACATTGA CTGGTTTATT
    GATATTTCCA ACAAGATAAG AGAGCTGGAT ATCTCTTCCA ACTGCCTTGC CTCCCTTCCA
    TCTGTGGTTC CATGGGGCCT TCTGAACCTG CAGAAACTGA ACATAAGTGA CAATCAGCTT
    TGTGACCTGC CCAGTGTACA GAGCTCTGAG GAAATCATCT GCTCAAAGTT ACTTGAGGTT
    GATGTCTCCA ATAATAAGCT GACATCACTT CCTTCTGCAT TCCTCCACCT GAGCCGGCTC
    CAGAGACTGT GTGCATCAAA AAACTATATT GTTGATCTCT TCAAAGAAGA TGGTACCAAC
    TGGATTGGTT TACGGAGGTT ACAGGATCTA GACTTGTCTG ATAATAGGAT CACAGAACTG
    CCAGCCTCCA TCCTGCACTG CTTCAAATCA CTCAGGAATC TAAATGTAGC TGGAAACAAG
    CTGAAGGTGT TACCTGAACC CTGGTCATGT CCACTTAAAT ATTGCAAGGC TTCAAAAAAT
    GCCATTGAGG GTCTCCCAGA GAACCTGGCT CTTTTCTGGA GGAATCATTT GGTTGAAGTT
    GATTTTTCTG AAAATGCCAT AAAGGAAGTT CCAAAGTCTC TCTTCCAGTT GGAGTGCTTG
    CAGTCTCTGA AGCTCTCCGG GAATCAACTG ACCACACTGC CTTCTTCTGA CACCTGGAAC
    TGCACAAATC TCAAGTGTCT AGATCTGTCC AAGAACCAGC TGGGAAGAAA TGAGGAAGGC
    ACTAGGACCA GACGTATGCC ATTTTTTTAC ACTAAGAGCA GAACAAAGAC AGGGACAGAT
    CCAGGTTCAG CAGTGGAATT TCCATTGTTT CTGAAGGAGT CACTGGAAGT TCTCTACCTA
    AATGACAACC AGTTGGAGTC TGTACCTCCA TCAATCTCTC AACTAAAGGG CCTTACAGAG
    CTCTACTTGG GAAACAATCC AGGTATACGA GAGCTTCCTG CAGATTTGGG GCAGTTGTCT
    AGTCTGTGGC AACTGGACCT TGAGGATCTG AACATCACAA ACCTGCCCGG GGAGATCAGA
    AAGGAGGGGC CAGTTACTAT CCTGGCTTAC CTGCGTGCTC AGCTGAGAAG GGCAGAACGA
    TGCCATCTGG TTAAAATGAT AATTGTGGGA CCCCCTCGGC AGGGCAAATC TACCCTTTTT
    GAGGTTCTGC AGAGTGGGAA AGTGTCTCAG CAAATGCAAG GGGACAACTC CATAAGAACC
    ACCTGCTGGG AGCTACAGAA GCCTGTGGGG ACCACTGCAA AGGTTGATTC TGTGCAGTTC
    CATGTGTGGG ACGTTGGAGG TTCGAATGCA ATGTCCGCTG TGAACCAGTG CTTCTTCACT
    GACAAAGCCT TGTATGTGGT AGTCTGGAAC CTTGCTCTTG GCGAGGAGGC TGTTGCCAAT
    CTTCAGTTCT GGCTACTGAA CATAGAGGCA AAAGCTCCCA ATGCCGTTGT TCTGGTTGTA
    GGGACGCACC TTGACTTCAT AGAAACCAAA TTCCGGTTGG AGAGAATTGC CACTCTTCGA
    GCTTATGTTC TGGCATTGTG TCGCTCCCCA TCTGGCTCCA GAGCAACTGG GTTCCCTGAT
    ATCACTTTCA AGCACTTACA TGAACTCTCT TGCAAAACAT TGGAGGGACT CGATGGTCTG
    CGCCAGCTGA TATTCCATGC TGCCTGCAAT ATGAGGGATG TAGGCAGTAC CATTGGATCC
    CAAAAACTAG TGGGAAGGCT GATCCCGAGA AGCTACCTGA ACCTTCAGGA GGCTGTGGTA
    GCTGAGCAGC AGAGACGAGC TCAAGGGGAT GAGGTACAAT ACCTGACTGA TGAGCAGATA
    GAACAGATTA TTGAACAGAG CCCTGGAAAC GATATACGCG ATTATGAAGA CATGCAGGCA
    GCGATCGGTT TCTTGATAGA AACTGGGACT TTGCTTCACT TTCCTGACAC TAGCCATGGC
    CTAAGAAACC TTTACTTCTT GGACCCTGTT TGGTTGTCAG AATGTTTGGA GAGGATTATT
    AACATTAAGA GCTCCAAGTC TGTGGCCCGG AATGGGGTGA TTAAGGCAGA AGATCTCAGA
    ATGCTGCTGG TTGGAACTGG ATTCACCAAA CAGACTGAAG AGCAATATTT TCAGTTCCTG
    GCAAAGTTTG AGATTGCTCT TCCTGTTGCC AATAACAGTT ACCTTTTGCC ACATTTGCTG
    CCGACAAAGC CCGGCCTGGA CATCCACAGC TTGCGGCAGC TTACTGGAAG CACAGTTCAG
    AGGTTGTTTA AGATGAGCTT TGTTCCTGTT GGGTTCTGGC AGCGGTTCAT AGCTCGGATG
    TTGATCAGTC TGGCAGAAAT GGATCTCCAG CTGTTTGAAA GCAAGAAAAA CACCAAGACA
    AAAAACAGGA AATCTGCAAT TTACAGCTTC ACTGGGTATC AGAGGAACCG ATGTAGCACC
    TTCCGAGTGA AGAGGAATCA GACCATTTAC TGGCAGGAAG GCCTAATGGT CACCTTTGAA
    GGGGGTTACC TCAGTGTTGA GTCATCAGAC ATTAACTGGA AGAAGAAGAA GAATGGTGGG
    ATTAAAATAA TTTGCCAGTC TGAGCTGAGA GATTTCTCTG CTATGGCATT TATCACAGAC
    CATGTGAACT CTCTCATAGA TCAGTGGTTC CCAGCCCTTA CAGCCACAGA GAGTGATGGC
    ACTTTACTGA TGGAGCAATG TGTGCCCTGC CACTGCTGTA CCACAGTCGC TAGTGATCAA
    GAGAAGAAAA ATAATGAGAA TACCCCACAT TACTTTAATA TGGAAGACTG CGTCCTAGCA
    GCTGTTGAGA AGGATTATTT GGAATGCCCG AACCACCCAG AACATGCAGT GCCACTGCAG
    GAACTGGTCC CAGAAATTTT CATGACTGAC TTTCCTTCAA GGCTTTTCCT TGACATCAAT
    GACTTAGAAT ACTCACAGGA GAGCTCCAAC ATTCTGGGTC AGGGAGGAAG TGGCACAGTG
    ATATACCGAG CTGAATATAA GCACAAGCCT GTGGCTGTGA AGATGTTTCA GGTTTCTAAG
    TGTCGGGCAT CTCCAGAGGT CATGCAAGAC ACCATGTTCA AGCACTTGCA GGCAATGGAC
    GCCATGAGAA ACTTCTCGGA ATTCCGTCAG GAGGCCAGCA TGCTCCATAC TCTGCAGCAC
    CCCTGCATTG TCTCCCTGAT TGGCATCAGC ATCCATCCAC TGTGCTTTGC CCTGGAGCTG
    GCACCTTTGG GGAGCCTCAA CACAGTGCTG GCAGGAACAG CCCAAGATGC AAGTTCATTC
    ATGCCTCTTG GTCACATGCT CACTCAGCGT ATTTCCTATC AAATTGCATC TGGGCTGTCC
    TACCTGCATA AAAAGAATAT AATATTTTGT GACCTGAAAT CAGATAACAT CCTGGTTTGG
    TCCCTGGATG TCAAGGAAGC TGTGATAATC AAACTCTCAG ACTATGGCAT TTCGCGGCTG
    TCATTCCATG AGGGAGCCCT GGGTGTGGAA GGCACCCCAG GATATCAAGC ACCCGAAATC
    CGTCCTCGGA TTGTGTATGA TGAAAAGGTG GATATGTTTT CATATGGTAT GGTCCTGTAT
    GAGCTATTGT CAGGCCAGAG ACCATCTTTG GGTCAGCACC AGCTGCAGAT TTCCAAGAAA
    CTGTCCAAGG GGATTCGTCC AGTTCTTGGA CGCCCAGAGG AAGTTCACTT CCATCATCTG
    CAGGACCTGA TGATGGAATG TTGGGACACG AAGCCTGAAA AGCGGCCTTT AGCTTCATCA
    GTTGTCCATC GTATGAATGA CCCCAGCTTC ACAGTTTTTA AGTACCAGTT GTCTTGTGGG
    ATACAGGAAT CATTCTGCTC ATCCAGAGAA CCAGAGAGCA CAGTTGTATT CTGGGTAGGA
    ACAGGGACAT CAAGGAATTA TACCGTGGTT GATGTTGAGA AAGGGCAGGT GGAAGTGCAG
    ACTCTGAGCT GCCCCGGGAT GAGAATGTCT TGCCAGCAAA GGGTACAGAA TACACTTTGG
    ATTGCAACAG AGGATCAGAA GATATCTATC TTTAGTCTGC AGGGCATGTG CCCCCTAAGT
    GTCGCACTAA AGGTGATAGA CACCCCTGCC ATTGTGACCT GCTTCCTTGT AGCGAATATG
    GAAAAGGGGA ACTGCCTGGT ATTTGCTGGC CTTTCAGACA GCCTGATTTC AGTGTTCCCA
    GTAAGTGGTG GCATCCCAGG CAGTGATTGC TCCTACATCT GTTCCCGCAC AGCTAATCGC
    TCCATGTTCA AGATAACAGA TGATGACCCA CGTCAGAAAC CTCACCCAGT GTTGGCCATG
    GAGATCTGCA ATAAATGTTC TGAGGTCTGG TATGGCAATG GACCTGGTGT CCTTGTAGTG
    GACTGTACCT CTCTTGAGAT TTCAAAAAGA CTGGAACCCT TTAGTGCACC ATCATCCATC
    ATCTCCATGG TTACAAGCAC AGACACTAAC AGAGAGGAGA CTGTTTGGTT ATTGGATGAT
    ATGACCAATT TTCTGGTCAT GTATAATGCT GCAGCCTACC AACTCTGTGC CCGATACTTC
    TGTGGTGATA GCAGTCCTCT AAGGGATATG TTTACAGTAA AGCCTTGTTC AGATGTGGTT
    CCTGTTGCAT TGCCAGTTAC AAACTGTAAT AGCCCCCGCT CAGCAAACGT CAGCATCATC
    TACAGCCTAG AACGTGGTAT GCAAGTTCTC AGCCACCAAG ACTCTCTTAC CGATTATTGC
    TCTGTGTCAT CCTTCTATTC TTCCATTCCT CAGGGGACTG CTAAGTCCTC CTCCAGTTTG
    CCAAGCACTC CGGTCAGCTA CAGTAGTGCC CCATTTTCCA CAGATAGTGA GGATCCAGAC
    CCAGCCCAAG AACTTATACA CAAAATTTCT AGTGAGAGCT GGGGGGGCAG AACACCTCTT
    TCAGAGGAGG AAAAACTCAA TGTGCAGGCT GTTGGGATTG TAGTCGTCAA AGATTTGCTC
    TGGGTTCCGA GGCGCGGAGG GGACATTATT ATCATTGGAC TGGAGAAGGA GTGTGGTGGA
    CAAAGAGGTC GTGTCATTGC TGTGTTACAA ACTGGCCTAG TAGAAAATCA AGGGGTCCAT
    CTCCACGCTG CTGCTCTTTG CAAGGATTCG GTTGTGTGCT GCTTGCAGAG TGAGACACTA
    GGGTGCTGCT TGTCCGTCTG GAGGAATTGG GGATCAGAGG AGTTTAACAT TTTTTATCAT
    GCCTCTGATG AACTTAGAAG GCTGGAAAAC TGCATGAGGA AAAGGAGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55222
    Clone ID Related Accession (Same CDS sequence) XM_012959577.3 , XM_012959578.3
    Accession Version XM_012959577.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6012bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959577.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGATGTACT GGTGTGTTGG AGTTGAAGCA TGTGCTGGAA GCAGAGGTGG AAGCATGGAT 
    TCATACAATG GCTCTGGAGG TATGGGGAAC AAACAAACCC TGCCAGGGGA GTTGCCAGTG
    AATCAGAGCC CAGTTATAGC AGACATACAG GCAGCATATT GCGAAGGGGA CAAAGAGAGA
    GCACAGAATC TAGTGAAAAG TGCCTGTGAG GAGAATGTCC AGAAGCATGA GAAGTACCAG
    TTTCTGAGAC TAACAGCAGC CTATGGAGAT CTTCCCTCTG TACGTTATCT GCTTAAGGAA
    GCACAGGTAG TTTTACCAAC TGAACCAAAT GATGACAATC CAGCAGTAAT GGCTTCGCAT
    TTTGGCCATG TTGACACAGT AACATTTTTA CTGGATTCTC TGCCAGATCC CTGCAGTCCC
    AAGCCACTGC TGAACTGGAT GCTGGTAACT GCCTGTGAGC AGGGTCACTT GGCTCTGGTT
    AAACTTTTGG TACTAAGATA CAATGCTGAC CCAGACTGTT ATGCTACAAG AAACAATGAG
    TTCCCTGTCC TCGGGAAATT ACCCCTTTAT GCCACCATTA TGGCAGGACA TGAAGAAGTT
    GCTGCCTTTC TGCTTCAAAA TGGAGCTGGC TTTTGTTCGT ACATACTGAT GGACAACCCA
    GAGCTGAGCA AAGTTTTACT GAGAAGAAGT CTTACCACTG AGCCACTAGA ACACAGTCAG
    CAGCAAGTAG CACAGGAGAT GGCTGTTTGT GTGAAGTGGT CTAGCCTTCG GCTGCCCTGG
    GTAGACATTG ACTGGTTTAT TGATATTTCC AACAAGATAA GAGAGCTGGA TATCTCTTCC
    AACTGCCTTG CCTCCCTTCC ATCTGTGGTT CCATGGGGCC TTCTGAACCT GCAGAAACTG
    AACATAAGTG ACAATCAGCT TTGTGACCTG CCCAGTGTAC AGAGCTCTGA GGAAATCATC
    TGCTCAAAGT TACTTGAGGT TGATGTCTCC AATAATAAGC TGACATCACT TCCTTCTGCA
    TTCCTCCACC TGAGCCGGCT CCAGAGACTG TGTGCATCAA AAAACTATAT TGTTGATCTC
    TTCAAAGAAG ATGGTACCAA CTGGATTGGT TTACGGAGGT TACAGGATCT AGACTTGCCT
    GATAATAGGA TCACAGAACT GCCAGCCTCC ATCCTGCACT GCTTCAAATC ACTCAGGAAT
    CTAAATGTAG CTGGAAACAA GCTGAAGGTG TTACCTGAAC CCTGGTCATG TCCACTTAAA
    TATTGCAAGG CTTCAAAAAA TGCCATTGAG GGTCTCCCAG AGAACCTGGC TCTTTTCTGG
    AGGAATCATT TGGTTGAAGT TGATTTTTCT GAAAATGCCA TAAAGGAAGT TCCAAAGTCT
    CTCTTCCAGT TGGAGTGCTT GCAGTCTCTG AAGCTCTCCG GGAATCAACT GACCACACTG
    CCTTCTTCTG ACACCTGGAA CTGCACAAAT CTCAAGTGTC TAGATCTGTC CAAGAACCAG
    CTGGGAAGAA ATGAGGAAGG CACTAGGACC AGACGTATGC CATTTTTTTA CACTAAGAGC
    AGAACAAAGA CAGGGACAGA TCCAGGTTCA GCAGTGGAAT TTCCATTGTT TCTGAAGGAG
    TCACTGGAAG TTCTCTACCT AAATGACAAC CAGTTGGAGT CTGTACCTCC ATCAGTCTCT
    CAACTAAAGG GCCTTACAGA GCTCTACTTG GGAAACAATC CAGGTATACG AGAGCTTCCT
    GCAGATTTGG GGCAGTTGTC TAGTCTGTGG CAACTGGACC TTGAGGATCT GAACATCACA
    AACCTGCCCG GGGAGATCAG AAAGGAGGGG CCAGTTACTA TCCTGGCTTA CCTGCGTGCT
    CAGCTGAGAA GGGCAGAACG ATGCCATCTG GTTAAAATGA TAATTGTGGG ACCCCCTCGG
    CAGGGCAAAT CTACCCTTTT TGAGGTTCTG CAGAGTGGGA AAGTGTCTCA GCAAATGCAA
    GGGGACAACT CCATAAGAAC CACCTGCTGG GAGCTACAGA AGCCTGTGGG GACCACTGCA
    AAGGTTGATT CTGTGCAGTT CCATGTGTGG GACGTTGGAG GTTCGAATGC AATGTCCGCT
    GTGAACCAGT GCTTCTTCAC TGACAAAGCC TTGTATGTGG TAGTCTGGAA CCTTGCTCTT
    GGCGAGGAGG CTGTTGCCAA TCTTCAGTTC TGGCTACTGA ACATAGAGGC AAAAGCTCCC
    AATGCCGTTG TTCTGGTTGT AGGGACGCAC CTTGACTTCA TAGAAACCAA ATTCCGGTTG
    GAGAGAATTG CCACTCTTCG AGCTTATGTT CTGGCATTGT GTCGCTCCCC ATCTGGCTCC
    AGAGCAACTG GGTTCCCTGA TATCACTTTC AAGCACTTAC ATGAACTCTC TTGCAAAACA
    TTGGAGGGAC TCGATGGTCT GCGCCAGCTG ATATTCCATG CTGCCTGCAA TATGAGGGAT
    GTAGGCAGTA CCATTGGATC CCAAAAACTA GTGGGAAGGC TGATCCCGAG AAGCTACCTG
    AACCTTCAGG AGGCTGTGGT AGCTGAGCAG CAGAGACGAG CTCAAGGGGA TGAGGTACAA
    TACCTGACTG ATGAGCAGAT AGAACAGATT ATTGAACAGA GCCCTGGAAA CGATATACGC
    GATTATGAAG ACATGCAGGC AGCGATCGGT TTCTTGATAG AAACTGGGAC TTTGCTTCAC
    TTTCCTGACA CTAGCCATGG CCTAAGAAAC CTTTACTTCT TGGACCCTGT TTGGTTGTCA
    GAATGTTTGG AGAGGATTAT TAACATTAAG AGCTCCAAGT CTGTGGCCCG GAATGGGGTG
    ATTAAGGCAG AAGATCTCAG AATGCTGCTG GTTGGAACTG GATTCACCAA ACAGACTGAA
    GAGCAATATT TTCAGTTCCT GGCAAAGTTT GAGATTGCTC TTCCTGTTGC CAATAACAGT
    TACCTTTTGC CACATTTGCT GCCGACAAAG CCCGGCCTGG ACATCCACAG CTTGCGGCAG
    CTTACTGGAA GCACAGTTCA GAGGTTGTTT AAGATGAGCT TTGTTCCTGT TGGGTTCTGG
    CAGCGGTTCA TAGCTCGGAT GTTGATCAGT CTGGCAGAAA TGGATCTCCA GCTGTTTGAA
    AGCAAGAAAA ACACCAAGAC AAAAAACAGG AAATCTGCAA TTTACAGCTT CACTGGGTAT
    CAGAGGAACC GATGTAGCAC CTTCCGAGTG AAGAGGAATC AGACCATTTA CTGGCAGGAA
    GGCCTAATGG TCACCTTTGA AGGGGGTTAC CTCAGTGTTG AGTCATCAGA CATTAACTGG
    AAGAAGAAGA AGAATGGTGG GATTAAAATA ATTTGCCAGT CTGAGCTGAG AGATTTCTCT
    GCTATGGCAT TTATCACAGA CCATGTGAAC TCTCTCATAG ATCAGTGGTT CCCAGCCCTT
    ACAGCCACAG AGAGTGATGG CACTTTACTG ATGGAGCAAT GTGTGCCCTG CCACTGCTGT
    ACCACAGTCG CTAGTGATCA AGAGAAGAAA AATAATGAGA ATACCCCACA TTACTTTAAT
    ATGGAAGACT GCGTCCTAGC AGCTGTTGAG AAGGATTATT TGGAATGCCC GAACCACCCA
    GAACATGCAG TGCCACTGCA GGAACTGGTC CCAGAAATTT TCATGACTGA CTTTCCTTCA
    AGGCTTTTCC TTGACATCAA TGACTTAGAA TACTCACAGG AGAGCTCCAA CATTCTGGGT
    CAGGGAGGAA GTGGCACAGT GATATACCGA GCTGAATATA AGCACAAGCC TGTGGCTGTG
    AAGATGTTTC AGGTTTCTAA GTGTCGGGCA TCTCCAGAGG TCATGCAAGA CACCATGTTC
    AAGCACTTGC AGGCAATGGA CGCCATGAGA AACTTCTCGG AATTCCGTCA GGAGGCCAGC
    ATGCTCCATA CTCTGCAGCA CCCCTGCATT GTCTCCCTGA TTGGCATCAG CATCCATCCA
    CTGTGCTTTG CCCTGGAGCT GGCACCTTTG GGGAGCCTCA ACACAGTGCT GGCAGGAACA
    GCCCAAGATG CAAGTTCATT CATGCCTCTT GGTCACATGC TCACTCAGCG TATTTCCTAT
    CAAATTGCAT CTGGGCTGTC CTACCTGCAT AAAAAGAATA TAATATTTTG TGACCTGAAA
    TCAGATAACA TCCTGGTTTG GTCCCTGGAT GTCAAGGAAG CTGTGATAAT CAAACTCTCA
    GACTATGGCA TTTCGCGGCT GTCATTCCAT GAGGGAGCCC TGGGTGTGGA AGGCACCCCA
    GGATATCAAG CACCCGAAAT CCGTCCTCGG ATTGTGTATG ATGAAAAGGT GGATATGTTT
    TCATATGGTA TGGTCCTGTA TGAGCTATTG TCAGGCCAGA GACCATCTTT GGGCCAGCAC
    CAGCTGCAGA TTTCCAAGAA ACTGTCCAAG GGGATTCGTC CAGTTCTTGG ACGCCCAGAG
    GAAGTTCACT TCCATCATCT GCAGGACCTG ATGATGGAAT GTTGGGACAC GAAGCCTGAA
    AAGCGGCCTT TAGCTTCATC AGTTGTCCAT CGTATGAATG ACCCCAGCTT CACAGTTTTT
    AAGTACCAGT TGTCTTGTGG GATACAGGAA TCATTCTGCT CATCCAGAGA ACCAGAGAGC
    ACAGTTGTAT TCTGGGTAGG AACAGGGACA TCAAGGAATT ATACCGTGGT TGATGTTGAG
    AAAGGGCAGG TGGAAGTGCA GACTCTGAGC TGCCCCGGGA TGAGAATGTC TTGCCAGCAA
    AGGGTACAGA ATACACTTTG GATTGCAACA GAGGATCAGA AGATATCTAT CTTTAGTCTG
    CAGGGCATGT GCCCCCTAAG TGTCGCACTA AAGGTGATAG ACACCCCTGC CATTGTGACC
    TGCTTCCTTG TAGCGAATAT GGAAAAGGGG AACTGCCTGG TATTTGCTGG CCTTTCAGAC
    AGCCTGATTT CAGTGTTCCC AGTAAGTGGT GGCATCCCAG GCAGTGATTG CTCCTACATC
    TGTTCCCGCA CAGCTAATCG CTCCATGTTC AAGATAACAG ATGATGACCC ACGTCAGAAA
    CCTCACCCAG TGTTGGCCAT GGAGATCTGC AATAAATGTT CTGAGGTCTG GTATGGCAAT
    GGACCTGGTG TCCTTGTAGT GGACTGTACC TCTCTTGAGA TTTCAAAAAG ACTGGAACCC
    TTTAGTGCAC CATCATCCAT CATCTCCATG GTTACAAGCA CAGACACTAA CAGAGAGGAG
    ACTGTTTGGT TATTGGATGA TATGACCAAT TTTCTGGTCA TGTATAATGC TGCAGCCTAC
    CAACTCTGTG CCCGATACTT CTGTGGTGAT AGCAGTCCTC TAAGGGATAT GTTTACAGTA
    AAGCCTTGTT CAGATGTGGT TCCTGTTGCA TTGCCAGTTA CAAACTGTAA TAGCCCCCGC
    TCAGCAAACG TCAGCATCAT CTACAGCCTA GAACGTGGTA TGCAAGTTCT CAGCCACCAA
    GACTCTCTTA CCGATTATTG CTCTGTGTCA TCCTTCTATT CTTCCATTCC TCAGGGGACT
    GCTAAGTCCT CCTCCAGTTT GCCAAGCACT CCGGTCAGCT ACAGTAGTGC CCCATTTTCC
    ACAGATAGTG AGGATCCAGA CCCAGCCCAA GAACTTATAC ACAAAATTTC TAGTGAGAGC
    TGGGGGGGCA GAACACCTCT TTCAGAGGAG GAAAAACTCA ATGTGCAGGC TGTTGGGATT
    GTAGTCGTCA AAGATTTGCT CTGGGTTCCG AGGCGCGGAG GGGACATTAT TATCATTGGA
    CTGGAGAAGG AGTGTGGTGG ACAAAGAGGT CGTGTCATTG CTGTGTTACA AACTGGCCTA
    GTAGAAAATC AAGGGGTCCA TCTCCACGCT GCTGCTCTTT GCAAGGATTC GGTTGTGTGC
    TGCTTGCAGA GTGAGACACT AGGGTGCTGC TTGTCCGTCT GGAGGAATTG GGGATCAGAG
    GAGTTTAACA TTTTTTATCA TGCCTCTGAT GAACTTAGAA GGCTGGAAAA CTGCATGAGG
    AAAAGGAGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815031.2
    CDS411..6422
    Translation

    Target ORF information:

    RefSeq Version XM_012959577.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis leucine-rich repeat kinase 1 (lrrk1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959577.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGATGTACT GGTGTGTTGG AGTTGAAGCA TGTGCTGGAA GCAGAGGTGG AAGCATGGAT 
    TCATACAATG GCTCTGGAGG TATGGGGAAC AAACAAACCC TGCCAGGGGA GTTGCCAGTG
    AATCAGAGCC CAGTTATAGC AGACATACAG GCAGCATATT GCGAAGGGGA CAAAGAGAGA
    GCACAGAATC TAGTGAAAAG TGCCTGTGAG GAGAATGTCC AGAAGCATGA GAAGTACCAG
    TTTCTGAGAC TAACAGCAGC CTATGGAGAT CTTCCCTCTG TACGTTATCT GCTTAAGGAA
    GCACAGGTAG TTTTACCAAC TGAACCAAAT GATGACAATC CAGCAGTAAT GGCTTCGCAT
    TTTGGCCATG TTGACACAGT AACATTTTTA CTGGATTCTC TGCCAGATCC CTGCAGTCCC
    AAGCCACTGC TGAACTGGAT GCTGGTAACT GCCTGTGAGC AGGGTCACTT GGCTCTGGTT
    AAACTTTTGG TACTAAGATA CAATGCTGAC CCAGACTGTT ATGCTACAAG AAACAATGAG
    TTCCCTGTCC TCGGGAAATT ACCCCTTTAT GCCACCATTA TGGCAGGACA TGAAGAAGTT
    GCTGCCTTTC TGCTTCAAAA TGGAGCTGGC TTTTGTTCGT ACATACTGAT GGACAACCCA
    GAGCTGAGCA AAGTTTTACT GAGAAGAAGT CTTACCACTG AGCCACTAGA ACACAGTCAG
    CAGCAAGTAG CACAGGAGAT GGCTGTTTGT GTGAAGTGGT CTAGCCTTCG GCTGCCCTGG
    GTAGACATTG ACTGGTTTAT TGATATTTCC AACAAGATAA GAGAGCTGGA TATCTCTTCC
    AACTGCCTTG CCTCCCTTCC ATCTGTGGTT CCATGGGGCC TTCTGAACCT GCAGAAACTG
    AACATAAGTG ACAATCAGCT TTGTGACCTG CCCAGTGTAC AGAGCTCTGA GGAAATCATC
    TGCTCAAAGT TACTTGAGGT TGATGTCTCC AATAATAAGC TGACATCACT TCCTTCTGCA
    TTCCTCCACC TGAGCCGGCT CCAGAGACTG TGTGCATCAA AAAACTATAT TGTTGATCTC
    TTCAAAGAAG ATGGTACCAA CTGGATTGGT TTACGGAGGT TACAGGATCT AGACTTGCCT
    GATAATAGGA TCACAGAACT GCCAGCCTCC ATCCTGCACT GCTTCAAATC ACTCAGGAAT
    CTAAATGTAG CTGGAAACAA GCTGAAGGTG TTACCTGAAC CCTGGTCATG TCCACTTAAA
    TATTGCAAGG CTTCAAAAAA TGCCATTGAG GGTCTCCCAG AGAACCTGGC TCTTTTCTGG
    AGGAATCATT TGGTTGAAGT TGATTTTTCT GAAAATGCCA TAAAGGAAGT TCCAAAGTCT
    CTCTTCCAGT TGGAGTGCTT GCAGTCTCTG AAGCTCTCCG GGAATCAACT GACCACACTG
    CCTTCTTCTG ACACCTGGAA CTGCACAAAT CTCAAGTGTC TAGATCTGTC CAAGAACCAG
    CTGGGAAGAA ATGAGGAAGG CACTAGGACC AGACGTATGC CATTTTTTTA CACTAAGAGC
    AGAACAAAGA CAGGGACAGA TCCAGGTTCA GCAGTGGAAT TTCCATTGTT TCTGAAGGAG
    TCACTGGAAG TTCTCTACCT AAATGACAAC CAGTTGGAGT CTGTACCTCC ATCAGTCTCT
    CAACTAAAGG GCCTTACAGA GCTCTACTTG GGAAACAATC CAGGTATACG AGAGCTTCCT
    GCAGATTTGG GGCAGTTGTC TAGTCTGTGG CAACTGGACC TTGAGGATCT GAACATCACA
    AACCTGCCCG GGGAGATCAG AAAGGAGGGG CCAGTTACTA TCCTGGCTTA CCTGCGTGCT
    CAGCTGAGAA GGGCAGAACG ATGCCATCTG GTTAAAATGA TAATTGTGGG ACCCCCTCGG
    CAGGGCAAAT CTACCCTTTT TGAGGTTCTG CAGAGTGGGA AAGTGTCTCA GCAAATGCAA
    GGGGACAACT CCATAAGAAC CACCTGCTGG GAGCTACAGA AGCCTGTGGG GACCACTGCA
    AAGGTTGATT CTGTGCAGTT CCATGTGTGG GACGTTGGAG GTTCGAATGC AATGTCCGCT
    GTGAACCAGT GCTTCTTCAC TGACAAAGCC TTGTATGTGG TAGTCTGGAA CCTTGCTCTT
    GGCGAGGAGG CTGTTGCCAA TCTTCAGTTC TGGCTACTGA ACATAGAGGC AAAAGCTCCC
    AATGCCGTTG TTCTGGTTGT AGGGACGCAC CTTGACTTCA TAGAAACCAA ATTCCGGTTG
    GAGAGAATTG CCACTCTTCG AGCTTATGTT CTGGCATTGT GTCGCTCCCC ATCTGGCTCC
    AGAGCAACTG GGTTCCCTGA TATCACTTTC AAGCACTTAC ATGAACTCTC TTGCAAAACA
    TTGGAGGGAC TCGATGGTCT GCGCCAGCTG ATATTCCATG CTGCCTGCAA TATGAGGGAT
    GTAGGCAGTA CCATTGGATC CCAAAAACTA GTGGGAAGGC TGATCCCGAG AAGCTACCTG
    AACCTTCAGG AGGCTGTGGT AGCTGAGCAG CAGAGACGAG CTCAAGGGGA TGAGGTACAA
    TACCTGACTG ATGAGCAGAT AGAACAGATT ATTGAACAGA GCCCTGGAAA CGATATACGC
    GATTATGAAG ACATGCAGGC AGCGATCGGT TTCTTGATAG AAACTGGGAC TTTGCTTCAC
    TTTCCTGACA CTAGCCATGG CCTAAGAAAC CTTTACTTCT TGGACCCTGT TTGGTTGTCA
    GAATGTTTGG AGAGGATTAT TAACATTAAG AGCTCCAAGT CTGTGGCCCG GAATGGGGTG
    ATTAAGGCAG AAGATCTCAG AATGCTGCTG GTTGGAACTG GATTCACCAA ACAGACTGAA
    GAGCAATATT TTCAGTTCCT GGCAAAGTTT GAGATTGCTC TTCCTGTTGC CAATAACAGT
    TACCTTTTGC CACATTTGCT GCCGACAAAG CCCGGCCTGG ACATCCACAG CTTGCGGCAG
    CTTACTGGAA GCACAGTTCA GAGGTTGTTT AAGATGAGCT TTGTTCCTGT TGGGTTCTGG
    CAGCGGTTCA TAGCTCGGAT GTTGATCAGT CTGGCAGAAA TGGATCTCCA GCTGTTTGAA
    AGCAAGAAAA ACACCAAGAC AAAAAACAGG AAATCTGCAA TTTACAGCTT CACTGGGTAT
    CAGAGGAACC GATGTAGCAC CTTCCGAGTG AAGAGGAATC AGACCATTTA CTGGCAGGAA
    GGCCTAATGG TCACCTTTGA AGGGGGTTAC CTCAGTGTTG AGTCATCAGA CATTAACTGG
    AAGAAGAAGA AGAATGGTGG GATTAAAATA ATTTGCCAGT CTGAGCTGAG AGATTTCTCT
    GCTATGGCAT TTATCACAGA CCATGTGAAC TCTCTCATAG ATCAGTGGTT CCCAGCCCTT
    ACAGCCACAG AGAGTGATGG CACTTTACTG ATGGAGCAAT GTGTGCCCTG CCACTGCTGT
    ACCACAGTCG CTAGTGATCA AGAGAAGAAA AATAATGAGA ATACCCCACA TTACTTTAAT
    ATGGAAGACT GCGTCCTAGC AGCTGTTGAG AAGGATTATT TGGAATGCCC GAACCACCCA
    GAACATGCAG TGCCACTGCA GGAACTGGTC CCAGAAATTT TCATGACTGA CTTTCCTTCA
    AGGCTTTTCC TTGACATCAA TGACTTAGAA TACTCACAGG AGAGCTCCAA CATTCTGGGT
    CAGGGAGGAA GTGGCACAGT GATATACCGA GCTGAATATA AGCACAAGCC TGTGGCTGTG
    AAGATGTTTC AGGTTTCTAA GTGTCGGGCA TCTCCAGAGG TCATGCAAGA CACCATGTTC
    AAGCACTTGC AGGCAATGGA CGCCATGAGA AACTTCTCGG AATTCCGTCA GGAGGCCAGC
    ATGCTCCATA CTCTGCAGCA CCCCTGCATT GTCTCCCTGA TTGGCATCAG CATCCATCCA
    CTGTGCTTTG CCCTGGAGCT GGCACCTTTG GGGAGCCTCA ACACAGTGCT GGCAGGAACA
    GCCCAAGATG CAAGTTCATT CATGCCTCTT GGTCACATGC TCACTCAGCG TATTTCCTAT
    CAAATTGCAT CTGGGCTGTC CTACCTGCAT AAAAAGAATA TAATATTTTG TGACCTGAAA
    TCAGATAACA TCCTGGTTTG GTCCCTGGAT GTCAAGGAAG CTGTGATAAT CAAACTCTCA
    GACTATGGCA TTTCGCGGCT GTCATTCCAT GAGGGAGCCC TGGGTGTGGA AGGCACCCCA
    GGATATCAAG CACCCGAAAT CCGTCCTCGG ATTGTGTATG ATGAAAAGGT GGATATGTTT
    TCATATGGTA TGGTCCTGTA TGAGCTATTG TCAGGCCAGA GACCATCTTT GGGCCAGCAC
    CAGCTGCAGA TTTCCAAGAA ACTGTCCAAG GGGATTCGTC CAGTTCTTGG ACGCCCAGAG
    GAAGTTCACT TCCATCATCT GCAGGACCTG ATGATGGAAT GTTGGGACAC GAAGCCTGAA
    AAGCGGCCTT TAGCTTCATC AGTTGTCCAT CGTATGAATG ACCCCAGCTT CACAGTTTTT
    AAGTACCAGT TGTCTTGTGG GATACAGGAA TCATTCTGCT CATCCAGAGA ACCAGAGAGC
    ACAGTTGTAT TCTGGGTAGG AACAGGGACA TCAAGGAATT ATACCGTGGT TGATGTTGAG
    AAAGGGCAGG TGGAAGTGCA GACTCTGAGC TGCCCCGGGA TGAGAATGTC TTGCCAGCAA
    AGGGTACAGA ATACACTTTG GATTGCAACA GAGGATCAGA AGATATCTAT CTTTAGTCTG
    CAGGGCATGT GCCCCCTAAG TGTCGCACTA AAGGTGATAG ACACCCCTGC CATTGTGACC
    TGCTTCCTTG TAGCGAATAT GGAAAAGGGG AACTGCCTGG TATTTGCTGG CCTTTCAGAC
    AGCCTGATTT CAGTGTTCCC AGTAAGTGGT GGCATCCCAG GCAGTGATTG CTCCTACATC
    TGTTCCCGCA CAGCTAATCG CTCCATGTTC AAGATAACAG ATGATGACCC ACGTCAGAAA
    CCTCACCCAG TGTTGGCCAT GGAGATCTGC AATAAATGTT CTGAGGTCTG GTATGGCAAT
    GGACCTGGTG TCCTTGTAGT GGACTGTACC TCTCTTGAGA TTTCAAAAAG ACTGGAACCC
    TTTAGTGCAC CATCATCCAT CATCTCCATG GTTACAAGCA CAGACACTAA CAGAGAGGAG
    ACTGTTTGGT TATTGGATGA TATGACCAAT TTTCTGGTCA TGTATAATGC TGCAGCCTAC
    CAACTCTGTG CCCGATACTT CTGTGGTGAT AGCAGTCCTC TAAGGGATAT GTTTACAGTA
    AAGCCTTGTT CAGATGTGGT TCCTGTTGCA TTGCCAGTTA CAAACTGTAA TAGCCCCCGC
    TCAGCAAACG TCAGCATCAT CTACAGCCTA GAACGTGGTA TGCAAGTTCT CAGCCACCAA
    GACTCTCTTA CCGATTATTG CTCTGTGTCA TCCTTCTATT CTTCCATTCC TCAGGGGACT
    GCTAAGTCCT CCTCCAGTTT GCCAAGCACT CCGGTCAGCT ACAGTAGTGC CCCATTTTCC
    ACAGATAGTG AGGATCCAGA CCCAGCCCAA GAACTTATAC ACAAAATTTC TAGTGAGAGC
    TGGGGGGGCA GAACACCTCT TTCAGAGGAG GAAAAACTCA ATGTGCAGGC TGTTGGGATT
    GTAGTCGTCA AAGATTTGCT CTGGGTTCCG AGGCGCGGAG GGGACATTAT TATCATTGGA
    CTGGAGAAGG AGTGTGGTGG ACAAAGAGGT CGTGTCATTG CTGTGTTACA AACTGGCCTA
    GTAGAAAATC AAGGGGTCCA TCTCCACGCT GCTGCTCTTT GCAAGGATTC GGTTGTGTGC
    TGCTTGCAGA GTGAGACACT AGGGTGCTGC TTGTCCGTCT GGAGGAATTG GGGATCAGAG
    GAGTTTAACA TTTTTTATCA TGCCTCTGAT GAACTTAGAA GGCTGGAAAA CTGCATGAGG
    AAAAGGAGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55222
    Clone ID Related Accession (Same CDS sequence) XM_012959577.3 , XM_012959578.3
    Accession Version XM_012959578.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6012bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959578.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGATGTACT GGTGTGTTGG AGTTGAAGCA TGTGCTGGAA GCAGAGGTGG AAGCATGGAT 
    TCATACAATG GCTCTGGAGG TATGGGGAAC AAACAAACCC TGCCAGGGGA GTTGCCAGTG
    AATCAGAGCC CAGTTATAGC AGACATACAG GCAGCATATT GCGAAGGGGA CAAAGAGAGA
    GCACAGAATC TAGTGAAAAG TGCCTGTGAG GAGAATGTCC AGAAGCATGA GAAGTACCAG
    TTTCTGAGAC TAACAGCAGC CTATGGAGAT CTTCCCTCTG TACGTTATCT GCTTAAGGAA
    GCACAGGTAG TTTTACCAAC TGAACCAAAT GATGACAATC CAGCAGTAAT GGCTTCGCAT
    TTTGGCCATG TTGACACAGT AACATTTTTA CTGGATTCTC TGCCAGATCC CTGCAGTCCC
    AAGCCACTGC TGAACTGGAT GCTGGTAACT GCCTGTGAGC AGGGTCACTT GGCTCTGGTT
    AAACTTTTGG TACTAAGATA CAATGCTGAC CCAGACTGTT ATGCTACAAG AAACAATGAG
    TTCCCTGTCC TCGGGAAATT ACCCCTTTAT GCCACCATTA TGGCAGGACA TGAAGAAGTT
    GCTGCCTTTC TGCTTCAAAA TGGAGCTGGC TTTTGTTCGT ACATACTGAT GGACAACCCA
    GAGCTGAGCA AAGTTTTACT GAGAAGAAGT CTTACCACTG AGCCACTAGA ACACAGTCAG
    CAGCAAGTAG CACAGGAGAT GGCTGTTTGT GTGAAGTGGT CTAGCCTTCG GCTGCCCTGG
    GTAGACATTG ACTGGTTTAT TGATATTTCC AACAAGATAA GAGAGCTGGA TATCTCTTCC
    AACTGCCTTG CCTCCCTTCC ATCTGTGGTT CCATGGGGCC TTCTGAACCT GCAGAAACTG
    AACATAAGTG ACAATCAGCT TTGTGACCTG CCCAGTGTAC AGAGCTCTGA GGAAATCATC
    TGCTCAAAGT TACTTGAGGT TGATGTCTCC AATAATAAGC TGACATCACT TCCTTCTGCA
    TTCCTCCACC TGAGCCGGCT CCAGAGACTG TGTGCATCAA AAAACTATAT TGTTGATCTC
    TTCAAAGAAG ATGGTACCAA CTGGATTGGT TTACGGAGGT TACAGGATCT AGACTTGCCT
    GATAATAGGA TCACAGAACT GCCAGCCTCC ATCCTGCACT GCTTCAAATC ACTCAGGAAT
    CTAAATGTAG CTGGAAACAA GCTGAAGGTG TTACCTGAAC CCTGGTCATG TCCACTTAAA
    TATTGCAAGG CTTCAAAAAA TGCCATTGAG GGTCTCCCAG AGAACCTGGC TCTTTTCTGG
    AGGAATCATT TGGTTGAAGT TGATTTTTCT GAAAATGCCA TAAAGGAAGT TCCAAAGTCT
    CTCTTCCAGT TGGAGTGCTT GCAGTCTCTG AAGCTCTCCG GGAATCAACT GACCACACTG
    CCTTCTTCTG ACACCTGGAA CTGCACAAAT CTCAAGTGTC TAGATCTGTC CAAGAACCAG
    CTGGGAAGAA ATGAGGAAGG CACTAGGACC AGACGTATGC CATTTTTTTA CACTAAGAGC
    AGAACAAAGA CAGGGACAGA TCCAGGTTCA GCAGTGGAAT TTCCATTGTT TCTGAAGGAG
    TCACTGGAAG TTCTCTACCT AAATGACAAC CAGTTGGAGT CTGTACCTCC ATCAGTCTCT
    CAACTAAAGG GCCTTACAGA GCTCTACTTG GGAAACAATC CAGGTATACG AGAGCTTCCT
    GCAGATTTGG GGCAGTTGTC TAGTCTGTGG CAACTGGACC TTGAGGATCT GAACATCACA
    AACCTGCCCG GGGAGATCAG AAAGGAGGGG CCAGTTACTA TCCTGGCTTA CCTGCGTGCT
    CAGCTGAGAA GGGCAGAACG ATGCCATCTG GTTAAAATGA TAATTGTGGG ACCCCCTCGG
    CAGGGCAAAT CTACCCTTTT TGAGGTTCTG CAGAGTGGGA AAGTGTCTCA GCAAATGCAA
    GGGGACAACT CCATAAGAAC CACCTGCTGG GAGCTACAGA AGCCTGTGGG GACCACTGCA
    AAGGTTGATT CTGTGCAGTT CCATGTGTGG GACGTTGGAG GTTCGAATGC AATGTCCGCT
    GTGAACCAGT GCTTCTTCAC TGACAAAGCC TTGTATGTGG TAGTCTGGAA CCTTGCTCTT
    GGCGAGGAGG CTGTTGCCAA TCTTCAGTTC TGGCTACTGA ACATAGAGGC AAAAGCTCCC
    AATGCCGTTG TTCTGGTTGT AGGGACGCAC CTTGACTTCA TAGAAACCAA ATTCCGGTTG
    GAGAGAATTG CCACTCTTCG AGCTTATGTT CTGGCATTGT GTCGCTCCCC ATCTGGCTCC
    AGAGCAACTG GGTTCCCTGA TATCACTTTC AAGCACTTAC ATGAACTCTC TTGCAAAACA
    TTGGAGGGAC TCGATGGTCT GCGCCAGCTG ATATTCCATG CTGCCTGCAA TATGAGGGAT
    GTAGGCAGTA CCATTGGATC CCAAAAACTA GTGGGAAGGC TGATCCCGAG AAGCTACCTG
    AACCTTCAGG AGGCTGTGGT AGCTGAGCAG CAGAGACGAG CTCAAGGGGA TGAGGTACAA
    TACCTGACTG ATGAGCAGAT AGAACAGATT ATTGAACAGA GCCCTGGAAA CGATATACGC
    GATTATGAAG ACATGCAGGC AGCGATCGGT TTCTTGATAG AAACTGGGAC TTTGCTTCAC
    TTTCCTGACA CTAGCCATGG CCTAAGAAAC CTTTACTTCT TGGACCCTGT TTGGTTGTCA
    GAATGTTTGG AGAGGATTAT TAACATTAAG AGCTCCAAGT CTGTGGCCCG GAATGGGGTG
    ATTAAGGCAG AAGATCTCAG AATGCTGCTG GTTGGAACTG GATTCACCAA ACAGACTGAA
    GAGCAATATT TTCAGTTCCT GGCAAAGTTT GAGATTGCTC TTCCTGTTGC CAATAACAGT
    TACCTTTTGC CACATTTGCT GCCGACAAAG CCCGGCCTGG ACATCCACAG CTTGCGGCAG
    CTTACTGGAA GCACAGTTCA GAGGTTGTTT AAGATGAGCT TTGTTCCTGT TGGGTTCTGG
    CAGCGGTTCA TAGCTCGGAT GTTGATCAGT CTGGCAGAAA TGGATCTCCA GCTGTTTGAA
    AGCAAGAAAA ACACCAAGAC AAAAAACAGG AAATCTGCAA TTTACAGCTT CACTGGGTAT
    CAGAGGAACC GATGTAGCAC CTTCCGAGTG AAGAGGAATC AGACCATTTA CTGGCAGGAA
    GGCCTAATGG TCACCTTTGA AGGGGGTTAC CTCAGTGTTG AGTCATCAGA CATTAACTGG
    AAGAAGAAGA AGAATGGTGG GATTAAAATA ATTTGCCAGT CTGAGCTGAG AGATTTCTCT
    GCTATGGCAT TTATCACAGA CCATGTGAAC TCTCTCATAG ATCAGTGGTT CCCAGCCCTT
    ACAGCCACAG AGAGTGATGG CACTTTACTG ATGGAGCAAT GTGTGCCCTG CCACTGCTGT
    ACCACAGTCG CTAGTGATCA AGAGAAGAAA AATAATGAGA ATACCCCACA TTACTTTAAT
    ATGGAAGACT GCGTCCTAGC AGCTGTTGAG AAGGATTATT TGGAATGCCC GAACCACCCA
    GAACATGCAG TGCCACTGCA GGAACTGGTC CCAGAAATTT TCATGACTGA CTTTCCTTCA
    AGGCTTTTCC TTGACATCAA TGACTTAGAA TACTCACAGG AGAGCTCCAA CATTCTGGGT
    CAGGGAGGAA GTGGCACAGT GATATACCGA GCTGAATATA AGCACAAGCC TGTGGCTGTG
    AAGATGTTTC AGGTTTCTAA GTGTCGGGCA TCTCCAGAGG TCATGCAAGA CACCATGTTC
    AAGCACTTGC AGGCAATGGA CGCCATGAGA AACTTCTCGG AATTCCGTCA GGAGGCCAGC
    ATGCTCCATA CTCTGCAGCA CCCCTGCATT GTCTCCCTGA TTGGCATCAG CATCCATCCA
    CTGTGCTTTG CCCTGGAGCT GGCACCTTTG GGGAGCCTCA ACACAGTGCT GGCAGGAACA
    GCCCAAGATG CAAGTTCATT CATGCCTCTT GGTCACATGC TCACTCAGCG TATTTCCTAT
    CAAATTGCAT CTGGGCTGTC CTACCTGCAT AAAAAGAATA TAATATTTTG TGACCTGAAA
    TCAGATAACA TCCTGGTTTG GTCCCTGGAT GTCAAGGAAG CTGTGATAAT CAAACTCTCA
    GACTATGGCA TTTCGCGGCT GTCATTCCAT GAGGGAGCCC TGGGTGTGGA AGGCACCCCA
    GGATATCAAG CACCCGAAAT CCGTCCTCGG ATTGTGTATG ATGAAAAGGT GGATATGTTT
    TCATATGGTA TGGTCCTGTA TGAGCTATTG TCAGGCCAGA GACCATCTTT GGGCCAGCAC
    CAGCTGCAGA TTTCCAAGAA ACTGTCCAAG GGGATTCGTC CAGTTCTTGG ACGCCCAGAG
    GAAGTTCACT TCCATCATCT GCAGGACCTG ATGATGGAAT GTTGGGACAC GAAGCCTGAA
    AAGCGGCCTT TAGCTTCATC AGTTGTCCAT CGTATGAATG ACCCCAGCTT CACAGTTTTT
    AAGTACCAGT TGTCTTGTGG GATACAGGAA TCATTCTGCT CATCCAGAGA ACCAGAGAGC
    ACAGTTGTAT TCTGGGTAGG AACAGGGACA TCAAGGAATT ATACCGTGGT TGATGTTGAG
    AAAGGGCAGG TGGAAGTGCA GACTCTGAGC TGCCCCGGGA TGAGAATGTC TTGCCAGCAA
    AGGGTACAGA ATACACTTTG GATTGCAACA GAGGATCAGA AGATATCTAT CTTTAGTCTG
    CAGGGCATGT GCCCCCTAAG TGTCGCACTA AAGGTGATAG ACACCCCTGC CATTGTGACC
    TGCTTCCTTG TAGCGAATAT GGAAAAGGGG AACTGCCTGG TATTTGCTGG CCTTTCAGAC
    AGCCTGATTT CAGTGTTCCC AGTAAGTGGT GGCATCCCAG GCAGTGATTG CTCCTACATC
    TGTTCCCGCA CAGCTAATCG CTCCATGTTC AAGATAACAG ATGATGACCC ACGTCAGAAA
    CCTCACCCAG TGTTGGCCAT GGAGATCTGC AATAAATGTT CTGAGGTCTG GTATGGCAAT
    GGACCTGGTG TCCTTGTAGT GGACTGTACC TCTCTTGAGA TTTCAAAAAG ACTGGAACCC
    TTTAGTGCAC CATCATCCAT CATCTCCATG GTTACAAGCA CAGACACTAA CAGAGAGGAG
    ACTGTTTGGT TATTGGATGA TATGACCAAT TTTCTGGTCA TGTATAATGC TGCAGCCTAC
    CAACTCTGTG CCCGATACTT CTGTGGTGAT AGCAGTCCTC TAAGGGATAT GTTTACAGTA
    AAGCCTTGTT CAGATGTGGT TCCTGTTGCA TTGCCAGTTA CAAACTGTAA TAGCCCCCGC
    TCAGCAAACG TCAGCATCAT CTACAGCCTA GAACGTGGTA TGCAAGTTCT CAGCCACCAA
    GACTCTCTTA CCGATTATTG CTCTGTGTCA TCCTTCTATT CTTCCATTCC TCAGGGGACT
    GCTAAGTCCT CCTCCAGTTT GCCAAGCACT CCGGTCAGCT ACAGTAGTGC CCCATTTTCC
    ACAGATAGTG AGGATCCAGA CCCAGCCCAA GAACTTATAC ACAAAATTTC TAGTGAGAGC
    TGGGGGGGCA GAACACCTCT TTCAGAGGAG GAAAAACTCA ATGTGCAGGC TGTTGGGATT
    GTAGTCGTCA AAGATTTGCT CTGGGTTCCG AGGCGCGGAG GGGACATTAT TATCATTGGA
    CTGGAGAAGG AGTGTGGTGG ACAAAGAGGT CGTGTCATTG CTGTGTTACA AACTGGCCTA
    GTAGAAAATC AAGGGGTCCA TCTCCACGCT GCTGCTCTTT GCAAGGATTC GGTTGTGTGC
    TGCTTGCAGA GTGAGACACT AGGGTGCTGC TTGTCCGTCT GGAGGAATTG GGGATCAGAG
    GAGTTTAACA TTTTTTATCA TGCCTCTGAT GAACTTAGAA GGCTGGAAAA CTGCATGAGG
    AAAAGGAGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815032.2
    CDS369..6380
    Translation

    Target ORF information:

    RefSeq Version XM_012959578.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis leucine-rich repeat kinase 1 (lrrk1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959578.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGATGTACT GGTGTGTTGG AGTTGAAGCA TGTGCTGGAA GCAGAGGTGG AAGCATGGAT 
    TCATACAATG GCTCTGGAGG TATGGGGAAC AAACAAACCC TGCCAGGGGA GTTGCCAGTG
    AATCAGAGCC CAGTTATAGC AGACATACAG GCAGCATATT GCGAAGGGGA CAAAGAGAGA
    GCACAGAATC TAGTGAAAAG TGCCTGTGAG GAGAATGTCC AGAAGCATGA GAAGTACCAG
    TTTCTGAGAC TAACAGCAGC CTATGGAGAT CTTCCCTCTG TACGTTATCT GCTTAAGGAA
    GCACAGGTAG TTTTACCAAC TGAACCAAAT GATGACAATC CAGCAGTAAT GGCTTCGCAT
    TTTGGCCATG TTGACACAGT AACATTTTTA CTGGATTCTC TGCCAGATCC CTGCAGTCCC
    AAGCCACTGC TGAACTGGAT GCTGGTAACT GCCTGTGAGC AGGGTCACTT GGCTCTGGTT
    AAACTTTTGG TACTAAGATA CAATGCTGAC CCAGACTGTT ATGCTACAAG AAACAATGAG
    TTCCCTGTCC TCGGGAAATT ACCCCTTTAT GCCACCATTA TGGCAGGACA TGAAGAAGTT
    GCTGCCTTTC TGCTTCAAAA TGGAGCTGGC TTTTGTTCGT ACATACTGAT GGACAACCCA
    GAGCTGAGCA AAGTTTTACT GAGAAGAAGT CTTACCACTG AGCCACTAGA ACACAGTCAG
    CAGCAAGTAG CACAGGAGAT GGCTGTTTGT GTGAAGTGGT CTAGCCTTCG GCTGCCCTGG
    GTAGACATTG ACTGGTTTAT TGATATTTCC AACAAGATAA GAGAGCTGGA TATCTCTTCC
    AACTGCCTTG CCTCCCTTCC ATCTGTGGTT CCATGGGGCC TTCTGAACCT GCAGAAACTG
    AACATAAGTG ACAATCAGCT TTGTGACCTG CCCAGTGTAC AGAGCTCTGA GGAAATCATC
    TGCTCAAAGT TACTTGAGGT TGATGTCTCC AATAATAAGC TGACATCACT TCCTTCTGCA
    TTCCTCCACC TGAGCCGGCT CCAGAGACTG TGTGCATCAA AAAACTATAT TGTTGATCTC
    TTCAAAGAAG ATGGTACCAA CTGGATTGGT TTACGGAGGT TACAGGATCT AGACTTGCCT
    GATAATAGGA TCACAGAACT GCCAGCCTCC ATCCTGCACT GCTTCAAATC ACTCAGGAAT
    CTAAATGTAG CTGGAAACAA GCTGAAGGTG TTACCTGAAC CCTGGTCATG TCCACTTAAA
    TATTGCAAGG CTTCAAAAAA TGCCATTGAG GGTCTCCCAG AGAACCTGGC TCTTTTCTGG
    AGGAATCATT TGGTTGAAGT TGATTTTTCT GAAAATGCCA TAAAGGAAGT TCCAAAGTCT
    CTCTTCCAGT TGGAGTGCTT GCAGTCTCTG AAGCTCTCCG GGAATCAACT GACCACACTG
    CCTTCTTCTG ACACCTGGAA CTGCACAAAT CTCAAGTGTC TAGATCTGTC CAAGAACCAG
    CTGGGAAGAA ATGAGGAAGG CACTAGGACC AGACGTATGC CATTTTTTTA CACTAAGAGC
    AGAACAAAGA CAGGGACAGA TCCAGGTTCA GCAGTGGAAT TTCCATTGTT TCTGAAGGAG
    TCACTGGAAG TTCTCTACCT AAATGACAAC CAGTTGGAGT CTGTACCTCC ATCAGTCTCT
    CAACTAAAGG GCCTTACAGA GCTCTACTTG GGAAACAATC CAGGTATACG AGAGCTTCCT
    GCAGATTTGG GGCAGTTGTC TAGTCTGTGG CAACTGGACC TTGAGGATCT GAACATCACA
    AACCTGCCCG GGGAGATCAG AAAGGAGGGG CCAGTTACTA TCCTGGCTTA CCTGCGTGCT
    CAGCTGAGAA GGGCAGAACG ATGCCATCTG GTTAAAATGA TAATTGTGGG ACCCCCTCGG
    CAGGGCAAAT CTACCCTTTT TGAGGTTCTG CAGAGTGGGA AAGTGTCTCA GCAAATGCAA
    GGGGACAACT CCATAAGAAC CACCTGCTGG GAGCTACAGA AGCCTGTGGG GACCACTGCA
    AAGGTTGATT CTGTGCAGTT CCATGTGTGG GACGTTGGAG GTTCGAATGC AATGTCCGCT
    GTGAACCAGT GCTTCTTCAC TGACAAAGCC TTGTATGTGG TAGTCTGGAA CCTTGCTCTT
    GGCGAGGAGG CTGTTGCCAA TCTTCAGTTC TGGCTACTGA ACATAGAGGC AAAAGCTCCC
    AATGCCGTTG TTCTGGTTGT AGGGACGCAC CTTGACTTCA TAGAAACCAA ATTCCGGTTG
    GAGAGAATTG CCACTCTTCG AGCTTATGTT CTGGCATTGT GTCGCTCCCC ATCTGGCTCC
    AGAGCAACTG GGTTCCCTGA TATCACTTTC AAGCACTTAC ATGAACTCTC TTGCAAAACA
    TTGGAGGGAC TCGATGGTCT GCGCCAGCTG ATATTCCATG CTGCCTGCAA TATGAGGGAT
    GTAGGCAGTA CCATTGGATC CCAAAAACTA GTGGGAAGGC TGATCCCGAG AAGCTACCTG
    AACCTTCAGG AGGCTGTGGT AGCTGAGCAG CAGAGACGAG CTCAAGGGGA TGAGGTACAA
    TACCTGACTG ATGAGCAGAT AGAACAGATT ATTGAACAGA GCCCTGGAAA CGATATACGC
    GATTATGAAG ACATGCAGGC AGCGATCGGT TTCTTGATAG AAACTGGGAC TTTGCTTCAC
    TTTCCTGACA CTAGCCATGG CCTAAGAAAC CTTTACTTCT TGGACCCTGT TTGGTTGTCA
    GAATGTTTGG AGAGGATTAT TAACATTAAG AGCTCCAAGT CTGTGGCCCG GAATGGGGTG
    ATTAAGGCAG AAGATCTCAG AATGCTGCTG GTTGGAACTG GATTCACCAA ACAGACTGAA
    GAGCAATATT TTCAGTTCCT GGCAAAGTTT GAGATTGCTC TTCCTGTTGC CAATAACAGT
    TACCTTTTGC CACATTTGCT GCCGACAAAG CCCGGCCTGG ACATCCACAG CTTGCGGCAG
    CTTACTGGAA GCACAGTTCA GAGGTTGTTT AAGATGAGCT TTGTTCCTGT TGGGTTCTGG
    CAGCGGTTCA TAGCTCGGAT GTTGATCAGT CTGGCAGAAA TGGATCTCCA GCTGTTTGAA
    AGCAAGAAAA ACACCAAGAC AAAAAACAGG AAATCTGCAA TTTACAGCTT CACTGGGTAT
    CAGAGGAACC GATGTAGCAC CTTCCGAGTG AAGAGGAATC AGACCATTTA CTGGCAGGAA
    GGCCTAATGG TCACCTTTGA AGGGGGTTAC CTCAGTGTTG AGTCATCAGA CATTAACTGG
    AAGAAGAAGA AGAATGGTGG GATTAAAATA ATTTGCCAGT CTGAGCTGAG AGATTTCTCT
    GCTATGGCAT TTATCACAGA CCATGTGAAC TCTCTCATAG ATCAGTGGTT CCCAGCCCTT
    ACAGCCACAG AGAGTGATGG CACTTTACTG ATGGAGCAAT GTGTGCCCTG CCACTGCTGT
    ACCACAGTCG CTAGTGATCA AGAGAAGAAA AATAATGAGA ATACCCCACA TTACTTTAAT
    ATGGAAGACT GCGTCCTAGC AGCTGTTGAG AAGGATTATT TGGAATGCCC GAACCACCCA
    GAACATGCAG TGCCACTGCA GGAACTGGTC CCAGAAATTT TCATGACTGA CTTTCCTTCA
    AGGCTTTTCC TTGACATCAA TGACTTAGAA TACTCACAGG AGAGCTCCAA CATTCTGGGT
    CAGGGAGGAA GTGGCACAGT GATATACCGA GCTGAATATA AGCACAAGCC TGTGGCTGTG
    AAGATGTTTC AGGTTTCTAA GTGTCGGGCA TCTCCAGAGG TCATGCAAGA CACCATGTTC
    AAGCACTTGC AGGCAATGGA CGCCATGAGA AACTTCTCGG AATTCCGTCA GGAGGCCAGC
    ATGCTCCATA CTCTGCAGCA CCCCTGCATT GTCTCCCTGA TTGGCATCAG CATCCATCCA
    CTGTGCTTTG CCCTGGAGCT GGCACCTTTG GGGAGCCTCA ACACAGTGCT GGCAGGAACA
    GCCCAAGATG CAAGTTCATT CATGCCTCTT GGTCACATGC TCACTCAGCG TATTTCCTAT
    CAAATTGCAT CTGGGCTGTC CTACCTGCAT AAAAAGAATA TAATATTTTG TGACCTGAAA
    TCAGATAACA TCCTGGTTTG GTCCCTGGAT GTCAAGGAAG CTGTGATAAT CAAACTCTCA
    GACTATGGCA TTTCGCGGCT GTCATTCCAT GAGGGAGCCC TGGGTGTGGA AGGCACCCCA
    GGATATCAAG CACCCGAAAT CCGTCCTCGG ATTGTGTATG ATGAAAAGGT GGATATGTTT
    TCATATGGTA TGGTCCTGTA TGAGCTATTG TCAGGCCAGA GACCATCTTT GGGCCAGCAC
    CAGCTGCAGA TTTCCAAGAA ACTGTCCAAG GGGATTCGTC CAGTTCTTGG ACGCCCAGAG
    GAAGTTCACT TCCATCATCT GCAGGACCTG ATGATGGAAT GTTGGGACAC GAAGCCTGAA
    AAGCGGCCTT TAGCTTCATC AGTTGTCCAT CGTATGAATG ACCCCAGCTT CACAGTTTTT
    AAGTACCAGT TGTCTTGTGG GATACAGGAA TCATTCTGCT CATCCAGAGA ACCAGAGAGC
    ACAGTTGTAT TCTGGGTAGG AACAGGGACA TCAAGGAATT ATACCGTGGT TGATGTTGAG
    AAAGGGCAGG TGGAAGTGCA GACTCTGAGC TGCCCCGGGA TGAGAATGTC TTGCCAGCAA
    AGGGTACAGA ATACACTTTG GATTGCAACA GAGGATCAGA AGATATCTAT CTTTAGTCTG
    CAGGGCATGT GCCCCCTAAG TGTCGCACTA AAGGTGATAG ACACCCCTGC CATTGTGACC
    TGCTTCCTTG TAGCGAATAT GGAAAAGGGG AACTGCCTGG TATTTGCTGG CCTTTCAGAC
    AGCCTGATTT CAGTGTTCCC AGTAAGTGGT GGCATCCCAG GCAGTGATTG CTCCTACATC
    TGTTCCCGCA CAGCTAATCG CTCCATGTTC AAGATAACAG ATGATGACCC ACGTCAGAAA
    CCTCACCCAG TGTTGGCCAT GGAGATCTGC AATAAATGTT CTGAGGTCTG GTATGGCAAT
    GGACCTGGTG TCCTTGTAGT GGACTGTACC TCTCTTGAGA TTTCAAAAAG ACTGGAACCC
    TTTAGTGCAC CATCATCCAT CATCTCCATG GTTACAAGCA CAGACACTAA CAGAGAGGAG
    ACTGTTTGGT TATTGGATGA TATGACCAAT TTTCTGGTCA TGTATAATGC TGCAGCCTAC
    CAACTCTGTG CCCGATACTT CTGTGGTGAT AGCAGTCCTC TAAGGGATAT GTTTACAGTA
    AAGCCTTGTT CAGATGTGGT TCCTGTTGCA TTGCCAGTTA CAAACTGTAA TAGCCCCCGC
    TCAGCAAACG TCAGCATCAT CTACAGCCTA GAACGTGGTA TGCAAGTTCT CAGCCACCAA
    GACTCTCTTA CCGATTATTG CTCTGTGTCA TCCTTCTATT CTTCCATTCC TCAGGGGACT
    GCTAAGTCCT CCTCCAGTTT GCCAAGCACT CCGGTCAGCT ACAGTAGTGC CCCATTTTCC
    ACAGATAGTG AGGATCCAGA CCCAGCCCAA GAACTTATAC ACAAAATTTC TAGTGAGAGC
    TGGGGGGGCA GAACACCTCT TTCAGAGGAG GAAAAACTCA ATGTGCAGGC TGTTGGGATT
    GTAGTCGTCA AAGATTTGCT CTGGGTTCCG AGGCGCGGAG GGGACATTAT TATCATTGGA
    CTGGAGAAGG AGTGTGGTGG ACAAAGAGGT CGTGTCATTG CTGTGTTACA AACTGGCCTA
    GTAGAAAATC AAGGGGTCCA TCTCCACGCT GCTGCTCTTT GCAAGGATTC GGTTGTGTGC
    TGCTTGCAGA GTGAGACACT AGGGTGCTGC TTGTCCGTCT GGAGGAATTG GGGATCAGAG
    GAGTTTAACA TTTTTTATCA TGCCTCTGAT GAACTTAGAA GGCTGGAAAA CTGCATGAGG
    AAAAGGAGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30975
    Clone ID Related Accession (Same CDS sequence) XM_012959577.2 , XM_012959578.2
    Accession Version XM_012959577.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6012bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959577.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGATGTACT GGTGTGTTGG AGTTGAAGCA TGTGCTGGAA GCAGAGGTGG AAGCATGGAT 
    TCATACAATG GCTCTGGAGG TATGGGGAAC AAACAAACCC TGCCAGGGGA GTTGCCAGTG
    AATCAGAGCC CAGTTATAGC AGACATACAG GCAGCATATT GCGAAGGGGA CAAAGAGAGA
    GCACAGAATC TAGTGAAAAG TGCCTGTGAG GAGAATGTCC AGAAGCATGA GAAGTACCAG
    TTTCTGAGAC TAACAGCAGC CTATGGAGAT CTTCCCTCTG TACGTTATCT GCTTAAGGAA
    GCACAGGTAG TTTTACCAAC TGAACCAAAT GATGACAATC CAGCAGTAAT GGCTTCGCAT
    TTTGGCCATG TTGACACAGT AACATTTTTA CTGGATTCTC TGCCAGATCC CTGCAGTCCC
    AAGCCACTGC TGAACTGGAT GCTGGTAACT GCCTGTGAGC AGGGTCACTT GGCTCTGGTT
    AAACTTTTGG TACTAAGATA CAATGCTGAC CCAGACTGTT ATGCTACAAG AAACAATGAG
    TTCCCTGTCC TCGGGAAATT ACCCCTTTAT GCCACCATTA TGGCAGGACA TGAAGAAGTT
    GCTGCCTTTC TGCTTCAAAA TGGAGCTGGC TTCTGTTCGT ACATACTGAT GGACAACCCA
    GAGCTGAGCA AAGTTTTACT GAGAAGAAGT CTTACCACTG AGCCACTAGA ACACAGTCAG
    CAGCAAGTAG CACAGGAGAT GGCTGTTTGT GTGAAGTGGT CTAGCCTTCG GCTGCCCTGG
    GTAGACATTG ACTGGTTTAT TGATATTTCC AACAAGATAA GAGAGCTGGA TATCTCTTCC
    AACTGCCTTG CCTCCCTTCC ATCTGTGGTT CCATGGGGCC TTCTGAACCT GCAGAAACTG
    AACATAAGTG ACAATCAGCT TTGTGACCTG CCCAGTGTAC AGAGCTCTGA GGAAATCATC
    TGCTCAAAGT TACTTGAGGT TGATGTCTCC AATAATAAGC TGACATCACT TCCTTCTGCA
    TTCCTCCACC TGAGCCGGCT CCAGAGACTG TGTGCATCAA AAAACTATAT TGTTGATCTC
    TTCAAAGAAG ATGGTACCAA CTGGATTGGT TTACGGAGGT TACAGGATCT AGACTTGTCT
    GATAATAGGA TCACAGAACT GCCAGCCTCC ATCCTGCACT GCTTCAAATC ACTCAGGAAT
    CTAAATGTAG CTGGAAACAA GCTGAAGGTG TTACCTGAAC CCTGGTCATG TCCACTTAAA
    TATTGCAAGG CTTCAAAAAA TGCCATTGAG GGTCTCCCAG AGAACCTGGC TCTTTTCTGG
    AGGAATCATT TGGTTGAAGT TGATTTTTCT GAAAATGCCA TAAAGGAAGT TCCAAAGTCT
    CTCTTCCAGT TGGAGTGCTT GCAGTCTCTG AAGCTCTCCG GGAATCAACT GACCACACTG
    CCTTCTTCTG ACACCTGGAA CTGCACAAAT CTCAAGTGTC TAGATCTGTC CAAGAACCAG
    CTGGGAAGAA ATGAGGAAGG CACTAGGACC AGACGTATGC CATTTTTTTA CACTAAGAGC
    AGAACAAAGA CAGGGACAGA TCCAGGTTCA GCAGTGGAAT TTCCATTGTT TCTGAAGGAG
    TCACTGGAAG TTCTCTACCT AAATGACAAC CAGTTGGAGT CTGTACCTCC ATCAATCTCT
    CAACTAAAGG GCCTTACAGA GCTCTACTTG GGAAACAATC CAGGTATACG AGAGCTTCCT
    GCAGATTTGG GGCAGTTGTC TAGTCTGTGG CAACTGGACC TTGAGGATCT GAACATCACA
    AACCTGCCCG GGGAGATCAG AAAGGAGGGG CCAGTTACTA TCCTGGCTTA CCTGCGTGCT
    CAGCTGAGAA GGGCAGAACG ATGCCATCTG GTTAAAATGA TAATTGTGGG ACCCCCTCGG
    CAGGGCAAAT CTACCCTTTT TGAGGTTCTG CAGAGTGGGA AAGTGTCTCA GCAAATGCAA
    GGGGACAACT CCATAAGAAC CACCTGCTGG GAGCTACAGA AGCCTGTGGG GACCACTGCA
    AAGGTTGATT CTGTGCAGTT CCATGTGTGG GACGTTGGAG GTTCGAATGC AATGTCCGCT
    GTGAACCAGT GCTTCTTCAC TGACAAAGCC TTGTATGTGG TAGTCTGGAA CCTTGCTCTT
    GGCGAGGAGG CTGTTGCCAA TCTTCAGTTC TGGCTACTGA ACATAGAGGC AAAAGCTCCC
    AATGCCGTTG TTCTGGTTGT AGGGACGCAC CTTGACTTCA TAGAAACCAA ATTCCGGTTG
    GAGAGAATTG CCACTCTTCG AGCTTATGTT CTGGCATTGT GTCGCTCCCC ATCTGGCTCC
    AGAGCAACTG GGTTCCCTGA TATCACTTTC AAGCACTTAC ATGAACTCTC TTGCAAAACA
    TTGGAGGGAC TCGATGGTCT GCGCCAGCTG ATATTCCATG CTGCCTGCAA TATGAGGGAT
    GTAGGCAGTA CCATTGGATC CCAAAAACTA GTGGGAAGGC TGATCCCGAG AAGCTACCTG
    AACCTTCAGG AGGCTGTGGT AGCTGAGCAG CAGAGACGAG CTCAAGGGGA TGAGGTACAA
    TACCTGACTG ATGAGCAGAT AGAACAGATT ATTGAACAGA GCCCTGGAAA CGATATACGC
    GATTATGAAG ACATGCAGGC AGCGATCGGT TTCTTGATAG AAACTGGGAC TTTGCTTCAC
    TTTCCTGACA CTAGCCATGG CCTAAGAAAC CTTTACTTCT TGGACCCTGT TTGGTTGTCA
    GAATGTTTGG AGAGGATTAT TAACATTAAG AGCTCCAAGT CTGTGGCCCG GAATGGGGTG
    ATTAAGGCAG AAGATCTCAG AATGCTGCTG GTTGGAACTG GATTCACCAA ACAGACTGAA
    GAGCAATATT TTCAGTTCCT GGCAAAGTTT GAGATTGCTC TTCCTGTTGC CAATAACAGT
    TACCTTTTGC CACATTTGCT GCCGACAAAG CCCGGCCTGG ACATCCACAG CTTGCGGCAG
    CTTACTGGAA GCACAGTTCA GAGGTTGTTT AAGATGAGCT TTGTTCCTGT TGGGTTCTGG
    CAGCGGTTCA TAGCTCGGAT GTTGATCAGT CTGGCAGAAA TGGATCTCCA GCTGTTTGAA
    AGCAAGAAAA ACACCAAGAC AAAAAACAGG AAATCTGCAA TTTACAGCTT CACTGGGTAT
    CAGAGGAACC GATGTAGCAC CTTCCGAGTG AAGAGGAATC AGACCATTTA CTGGCAGGAA
    GGCCTAATGG TCACCTTTGA AGGGGGTTAC CTCAGTGTTG AGTCATCAGA CATTAACTGG
    AAGAAGAAGA AGAATGGTGG GATTAAAATA ATTTGCCAGT CTGAGCTGAG AGATTTCTCT
    GCTATGGCAT TTATCACAGA CCATGTGAAC TCTCTCATAG ATCAGTGGTT CCCAGCCCTT
    ACAGCCACAG AGAGTGATGG CACTTTACTG ATGGAGCAAT GTGTGCCCTG CCACTGCTGT
    ACCACAGTCG CTAGTGATCA AGAGAAGAAA AATAATGAGA ATACCCCACA TTACTTTAAT
    ATGGAAGACT GCGTCCTAGC AGCTGTTGAG AAGGATTATT TGGAATGCCC GAACCACCCA
    GAACATGCAG TGCCACTGCA GGAACTGGTC CCAGAAATTT TCATGACTGA CTTTCCTTCA
    AGGCTTTTCC TTGACATCAA TGACTTAGAA TACTCACAGG AGAGCTCCAA CATTCTGGGT
    CAGGGAGGAA GTGGCACAGT GATATACCGA GCTGAATATA AGCACAAGCC TGTGGCTGTG
    AAGATGTTTC AGGTTTCTAA GTGTCGGGCA TCTCCAGAGG TCATGCAAGA CACCATGTTC
    AAGCACTTGC AGGCAATGGA CGCCATGAGA AACTTCTCGG AATTCCGTCA GGAGGCCAGC
    ATGCTCCATA CTCTGCAGCA CCCCTGCATT GTCTCCCTGA TTGGCATCAG CATCCATCCA
    CTGTGCTTTG CCCTGGAGCT GGCACCTTTG GGGAGCCTCA ACACAGTGCT GGCAGGAACA
    GCCCAAGATG CAAGTTCATT CATGCCTCTT GGTCACATGC TCACTCAGCG TATTTCCTAT
    CAAATTGCAT CTGGGCTGTC CTACCTGCAT AAAAAGAATA TAATATTTTG TGACCTGAAA
    TCAGATAACA TCCTGGTTTG GTCCCTGGAT GTCAAGGAAG CTGTGATAAT CAAACTCTCA
    GACTATGGCA TTTCGCGGCT GTCATTCCAT GAGGGAGCCC TGGGTGTGGA AGGCACCCCA
    GGATATCAAG CACCCGAAAT CCGTCCTCGG ATTGTGTATG ATGAAAAGGT GGATATGTTT
    TCATATGGTA TGGTCCTGTA TGAGCTATTG TCAGGCCAGA GACCATCTTT GGGTCAGCAC
    CAGCTGCAGA TTTCCAAGAA ACTGTCCAAG GGGATTCGTC CAGTTCTTGG ACGCCCAGAG
    GAAGTTCACT TCCATCATCT GCAGGACCTG ATGATGGAAT GTTGGGACAC GAAGCCTGAA
    AAGCGGCCTT TAGCTTCATC AGTTGTCCAT CGTATGAATG ACCCCAGCTT CACAGTTTTT
    AAGTACCAGT TGTCTTGTGG GATACAGGAA TCATTCTGCT CATCCAGAGA ACCAGAGAGC
    ACAGTTGTAT TCTGGGTAGG AACAGGGACA TCAAGGAATT ATACCGTGGT TGATGTTGAG
    AAAGGGCAGG TGGAAGTGCA GACTCTGAGC TGCCCCGGGA TGAGAATGTC TTGCCAGCAA
    AGGGTACAGA ATACACTTTG GATTGCAACA GAGGATCAGA AGATATCTAT CTTTAGTCTG
    CAGGGCATGT GCCCCCTAAG TGTCGCACTA AAGGTGATAG ACACCCCTGC CATTGTGACC
    TGCTTCCTTG TAGCGAATAT GGAAAAGGGG AACTGCCTGG TATTTGCTGG CCTTTCAGAC
    AGCCTGATTT CAGTGTTCCC AGTAAGTGGT GGCATCCCAG GCAGTGATTG CTCCTACATC
    TGTTCCCGCA CAGCTAATCG CTCCATGTTC AAGATAACAG ATGATGACCC ACGTCAGAAA
    CCTCACCCAG TGTTGGCCAT GGAGATCTGC AATAAATGTT CTGAGGTCTG GTATGGCAAT
    GGACCTGGTG TCCTTGTAGT GGACTGTACC TCTCTTGAGA TTTCAAAAAG ACTGGAACCC
    TTTAGTGCAC CATCATCCAT CATCTCCATG GTTACAAGCA CAGACACTAA CAGAGAGGAG
    ACTGTTTGGT TATTGGATGA TATGACCAAT TTTCTGGTCA TGTATAATGC TGCAGCCTAC
    CAACTCTGTG CCCGATACTT CTGTGGTGAT AGCAGTCCTC TAAGGGATAT GTTTACAGTA
    AAGCCTTGTT CAGATGTGGT TCCTGTTGCA TTGCCAGTTA CAAACTGTAA TAGCCCCCGC
    TCAGCAAACG TCAGCATCAT CTACAGCCTA GAACGTGGTA TGCAAGTTCT CAGCCACCAA
    GACTCTCTTA CCGATTATTG CTCTGTGTCA TCCTTCTATT CTTCCATTCC TCAGGGGACT
    GCTAAGTCCT CCTCCAGTTT GCCAAGCACT CCGGTCAGCT ACAGTAGTGC CCCATTTTCC
    ACAGATAGTG AGGATCCAGA CCCAGCCCAA GAACTTATAC ACAAAATTTC TAGTGAGAGC
    TGGGGGGGCA GAACACCTCT TTCAGAGGAG GAAAAACTCA ATGTGCAGGC TGTTGGGATT
    GTAGTCGTCA AAGATTTGCT CTGGGTTCCG AGGCGCGGAG GGGACATTAT TATCATTGGA
    CTGGAGAAGG AGTGTGGTGG ACAAAGAGGT CGTGTCATTG CTGTGTTACA AACTGGCCTA
    GTAGAAAATC AAGGGGTCCA TCTCCACGCT GCTGCTCTTT GCAAGGATTC GGTTGTGTGC
    TGCTTGCAGA GTGAGACACT AGGGTGCTGC TTGTCCGTCT GGAGGAATTG GGGATCAGAG
    GAGTTTAACA TTTTTTATCA TGCCTCTGAT GAACTTAGAA GGCTGGAAAA CTGCATGAGG
    AAAAGGAGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815031.1
    CDS445..6456
    Translation

    Target ORF information:

    RefSeq Version XM_012959577.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis leucine-rich repeat kinase 1 (lrrk1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959577.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGATGTACT GGTGTGTTGG AGTTGAAGCA TGTGCTGGAA GCAGAGGTGG AAGCATGGAT 
    TCATACAATG GCTCTGGAGG TATGGGGAAC AAACAAACCC TGCCAGGGGA GTTGCCAGTG
    AATCAGAGCC CAGTTATAGC AGACATACAG GCAGCATATT GCGAAGGGGA CAAAGAGAGA
    GCACAGAATC TAGTGAAAAG TGCCTGTGAG GAGAATGTCC AGAAGCATGA GAAGTACCAG
    TTTCTGAGAC TAACAGCAGC CTATGGAGAT CTTCCCTCTG TACGTTATCT GCTTAAGGAA
    GCACAGGTAG TTTTACCAAC TGAACCAAAT GATGACAATC CAGCAGTAAT GGCTTCGCAT
    TTTGGCCATG TTGACACAGT AACATTTTTA CTGGATTCTC TGCCAGATCC CTGCAGTCCC
    AAGCCACTGC TGAACTGGAT GCTGGTAACT GCCTGTGAGC AGGGTCACTT GGCTCTGGTT
    AAACTTTTGG TACTAAGATA CAATGCTGAC CCAGACTGTT ATGCTACAAG AAACAATGAG
    TTCCCTGTCC TCGGGAAATT ACCCCTTTAT GCCACCATTA TGGCAGGACA TGAAGAAGTT
    GCTGCCTTTC TGCTTCAAAA TGGAGCTGGC TTCTGTTCGT ACATACTGAT GGACAACCCA
    GAGCTGAGCA AAGTTTTACT GAGAAGAAGT CTTACCACTG AGCCACTAGA ACACAGTCAG
    CAGCAAGTAG CACAGGAGAT GGCTGTTTGT GTGAAGTGGT CTAGCCTTCG GCTGCCCTGG
    GTAGACATTG ACTGGTTTAT TGATATTTCC AACAAGATAA GAGAGCTGGA TATCTCTTCC
    AACTGCCTTG CCTCCCTTCC ATCTGTGGTT CCATGGGGCC TTCTGAACCT GCAGAAACTG
    AACATAAGTG ACAATCAGCT TTGTGACCTG CCCAGTGTAC AGAGCTCTGA GGAAATCATC
    TGCTCAAAGT TACTTGAGGT TGATGTCTCC AATAATAAGC TGACATCACT TCCTTCTGCA
    TTCCTCCACC TGAGCCGGCT CCAGAGACTG TGTGCATCAA AAAACTATAT TGTTGATCTC
    TTCAAAGAAG ATGGTACCAA CTGGATTGGT TTACGGAGGT TACAGGATCT AGACTTGTCT
    GATAATAGGA TCACAGAACT GCCAGCCTCC ATCCTGCACT GCTTCAAATC ACTCAGGAAT
    CTAAATGTAG CTGGAAACAA GCTGAAGGTG TTACCTGAAC CCTGGTCATG TCCACTTAAA
    TATTGCAAGG CTTCAAAAAA TGCCATTGAG GGTCTCCCAG AGAACCTGGC TCTTTTCTGG
    AGGAATCATT TGGTTGAAGT TGATTTTTCT GAAAATGCCA TAAAGGAAGT TCCAAAGTCT
    CTCTTCCAGT TGGAGTGCTT GCAGTCTCTG AAGCTCTCCG GGAATCAACT GACCACACTG
    CCTTCTTCTG ACACCTGGAA CTGCACAAAT CTCAAGTGTC TAGATCTGTC CAAGAACCAG
    CTGGGAAGAA ATGAGGAAGG CACTAGGACC AGACGTATGC CATTTTTTTA CACTAAGAGC
    AGAACAAAGA CAGGGACAGA TCCAGGTTCA GCAGTGGAAT TTCCATTGTT TCTGAAGGAG
    TCACTGGAAG TTCTCTACCT AAATGACAAC CAGTTGGAGT CTGTACCTCC ATCAATCTCT
    CAACTAAAGG GCCTTACAGA GCTCTACTTG GGAAACAATC CAGGTATACG AGAGCTTCCT
    GCAGATTTGG GGCAGTTGTC TAGTCTGTGG CAACTGGACC TTGAGGATCT GAACATCACA
    AACCTGCCCG GGGAGATCAG AAAGGAGGGG CCAGTTACTA TCCTGGCTTA CCTGCGTGCT
    CAGCTGAGAA GGGCAGAACG ATGCCATCTG GTTAAAATGA TAATTGTGGG ACCCCCTCGG
    CAGGGCAAAT CTACCCTTTT TGAGGTTCTG CAGAGTGGGA AAGTGTCTCA GCAAATGCAA
    GGGGACAACT CCATAAGAAC CACCTGCTGG GAGCTACAGA AGCCTGTGGG GACCACTGCA
    AAGGTTGATT CTGTGCAGTT CCATGTGTGG GACGTTGGAG GTTCGAATGC AATGTCCGCT
    GTGAACCAGT GCTTCTTCAC TGACAAAGCC TTGTATGTGG TAGTCTGGAA CCTTGCTCTT
    GGCGAGGAGG CTGTTGCCAA TCTTCAGTTC TGGCTACTGA ACATAGAGGC AAAAGCTCCC
    AATGCCGTTG TTCTGGTTGT AGGGACGCAC CTTGACTTCA TAGAAACCAA ATTCCGGTTG
    GAGAGAATTG CCACTCTTCG AGCTTATGTT CTGGCATTGT GTCGCTCCCC ATCTGGCTCC
    AGAGCAACTG GGTTCCCTGA TATCACTTTC AAGCACTTAC ATGAACTCTC TTGCAAAACA
    TTGGAGGGAC TCGATGGTCT GCGCCAGCTG ATATTCCATG CTGCCTGCAA TATGAGGGAT
    GTAGGCAGTA CCATTGGATC CCAAAAACTA GTGGGAAGGC TGATCCCGAG AAGCTACCTG
    AACCTTCAGG AGGCTGTGGT AGCTGAGCAG CAGAGACGAG CTCAAGGGGA TGAGGTACAA
    TACCTGACTG ATGAGCAGAT AGAACAGATT ATTGAACAGA GCCCTGGAAA CGATATACGC
    GATTATGAAG ACATGCAGGC AGCGATCGGT TTCTTGATAG AAACTGGGAC TTTGCTTCAC
    TTTCCTGACA CTAGCCATGG CCTAAGAAAC CTTTACTTCT TGGACCCTGT TTGGTTGTCA
    GAATGTTTGG AGAGGATTAT TAACATTAAG AGCTCCAAGT CTGTGGCCCG GAATGGGGTG
    ATTAAGGCAG AAGATCTCAG AATGCTGCTG GTTGGAACTG GATTCACCAA ACAGACTGAA
    GAGCAATATT TTCAGTTCCT GGCAAAGTTT GAGATTGCTC TTCCTGTTGC CAATAACAGT
    TACCTTTTGC CACATTTGCT GCCGACAAAG CCCGGCCTGG ACATCCACAG CTTGCGGCAG
    CTTACTGGAA GCACAGTTCA GAGGTTGTTT AAGATGAGCT TTGTTCCTGT TGGGTTCTGG
    CAGCGGTTCA TAGCTCGGAT GTTGATCAGT CTGGCAGAAA TGGATCTCCA GCTGTTTGAA
    AGCAAGAAAA ACACCAAGAC AAAAAACAGG AAATCTGCAA TTTACAGCTT CACTGGGTAT
    CAGAGGAACC GATGTAGCAC CTTCCGAGTG AAGAGGAATC AGACCATTTA CTGGCAGGAA
    GGCCTAATGG TCACCTTTGA AGGGGGTTAC CTCAGTGTTG AGTCATCAGA CATTAACTGG
    AAGAAGAAGA AGAATGGTGG GATTAAAATA ATTTGCCAGT CTGAGCTGAG AGATTTCTCT
    GCTATGGCAT TTATCACAGA CCATGTGAAC TCTCTCATAG ATCAGTGGTT CCCAGCCCTT
    ACAGCCACAG AGAGTGATGG CACTTTACTG ATGGAGCAAT GTGTGCCCTG CCACTGCTGT
    ACCACAGTCG CTAGTGATCA AGAGAAGAAA AATAATGAGA ATACCCCACA TTACTTTAAT
    ATGGAAGACT GCGTCCTAGC AGCTGTTGAG AAGGATTATT TGGAATGCCC GAACCACCCA
    GAACATGCAG TGCCACTGCA GGAACTGGTC CCAGAAATTT TCATGACTGA CTTTCCTTCA
    AGGCTTTTCC TTGACATCAA TGACTTAGAA TACTCACAGG AGAGCTCCAA CATTCTGGGT
    CAGGGAGGAA GTGGCACAGT GATATACCGA GCTGAATATA AGCACAAGCC TGTGGCTGTG
    AAGATGTTTC AGGTTTCTAA GTGTCGGGCA TCTCCAGAGG TCATGCAAGA CACCATGTTC
    AAGCACTTGC AGGCAATGGA CGCCATGAGA AACTTCTCGG AATTCCGTCA GGAGGCCAGC
    ATGCTCCATA CTCTGCAGCA CCCCTGCATT GTCTCCCTGA TTGGCATCAG CATCCATCCA
    CTGTGCTTTG CCCTGGAGCT GGCACCTTTG GGGAGCCTCA ACACAGTGCT GGCAGGAACA
    GCCCAAGATG CAAGTTCATT CATGCCTCTT GGTCACATGC TCACTCAGCG TATTTCCTAT
    CAAATTGCAT CTGGGCTGTC CTACCTGCAT AAAAAGAATA TAATATTTTG TGACCTGAAA
    TCAGATAACA TCCTGGTTTG GTCCCTGGAT GTCAAGGAAG CTGTGATAAT CAAACTCTCA
    GACTATGGCA TTTCGCGGCT GTCATTCCAT GAGGGAGCCC TGGGTGTGGA AGGCACCCCA
    GGATATCAAG CACCCGAAAT CCGTCCTCGG ATTGTGTATG ATGAAAAGGT GGATATGTTT
    TCATATGGTA TGGTCCTGTA TGAGCTATTG TCAGGCCAGA GACCATCTTT GGGTCAGCAC
    CAGCTGCAGA TTTCCAAGAA ACTGTCCAAG GGGATTCGTC CAGTTCTTGG ACGCCCAGAG
    GAAGTTCACT TCCATCATCT GCAGGACCTG ATGATGGAAT GTTGGGACAC GAAGCCTGAA
    AAGCGGCCTT TAGCTTCATC AGTTGTCCAT CGTATGAATG ACCCCAGCTT CACAGTTTTT
    AAGTACCAGT TGTCTTGTGG GATACAGGAA TCATTCTGCT CATCCAGAGA ACCAGAGAGC
    ACAGTTGTAT TCTGGGTAGG AACAGGGACA TCAAGGAATT ATACCGTGGT TGATGTTGAG
    AAAGGGCAGG TGGAAGTGCA GACTCTGAGC TGCCCCGGGA TGAGAATGTC TTGCCAGCAA
    AGGGTACAGA ATACACTTTG GATTGCAACA GAGGATCAGA AGATATCTAT CTTTAGTCTG
    CAGGGCATGT GCCCCCTAAG TGTCGCACTA AAGGTGATAG ACACCCCTGC CATTGTGACC
    TGCTTCCTTG TAGCGAATAT GGAAAAGGGG AACTGCCTGG TATTTGCTGG CCTTTCAGAC
    AGCCTGATTT CAGTGTTCCC AGTAAGTGGT GGCATCCCAG GCAGTGATTG CTCCTACATC
    TGTTCCCGCA CAGCTAATCG CTCCATGTTC AAGATAACAG ATGATGACCC ACGTCAGAAA
    CCTCACCCAG TGTTGGCCAT GGAGATCTGC AATAAATGTT CTGAGGTCTG GTATGGCAAT
    GGACCTGGTG TCCTTGTAGT GGACTGTACC TCTCTTGAGA TTTCAAAAAG ACTGGAACCC
    TTTAGTGCAC CATCATCCAT CATCTCCATG GTTACAAGCA CAGACACTAA CAGAGAGGAG
    ACTGTTTGGT TATTGGATGA TATGACCAAT TTTCTGGTCA TGTATAATGC TGCAGCCTAC
    CAACTCTGTG CCCGATACTT CTGTGGTGAT AGCAGTCCTC TAAGGGATAT GTTTACAGTA
    AAGCCTTGTT CAGATGTGGT TCCTGTTGCA TTGCCAGTTA CAAACTGTAA TAGCCCCCGC
    TCAGCAAACG TCAGCATCAT CTACAGCCTA GAACGTGGTA TGCAAGTTCT CAGCCACCAA
    GACTCTCTTA CCGATTATTG CTCTGTGTCA TCCTTCTATT CTTCCATTCC TCAGGGGACT
    GCTAAGTCCT CCTCCAGTTT GCCAAGCACT CCGGTCAGCT ACAGTAGTGC CCCATTTTCC
    ACAGATAGTG AGGATCCAGA CCCAGCCCAA GAACTTATAC ACAAAATTTC TAGTGAGAGC
    TGGGGGGGCA GAACACCTCT TTCAGAGGAG GAAAAACTCA ATGTGCAGGC TGTTGGGATT
    GTAGTCGTCA AAGATTTGCT CTGGGTTCCG AGGCGCGGAG GGGACATTAT TATCATTGGA
    CTGGAGAAGG AGTGTGGTGG ACAAAGAGGT CGTGTCATTG CTGTGTTACA AACTGGCCTA
    GTAGAAAATC AAGGGGTCCA TCTCCACGCT GCTGCTCTTT GCAAGGATTC GGTTGTGTGC
    TGCTTGCAGA GTGAGACACT AGGGTGCTGC TTGTCCGTCT GGAGGAATTG GGGATCAGAG
    GAGTTTAACA TTTTTTATCA TGCCTCTGAT GAACTTAGAA GGCTGGAAAA CTGCATGAGG
    AAAAGGAGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30975
    Clone ID Related Accession (Same CDS sequence) XM_012959577.2 , XM_012959578.2
    Accession Version XM_012959578.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6012bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959578.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGATGTACT GGTGTGTTGG AGTTGAAGCA TGTGCTGGAA GCAGAGGTGG AAGCATGGAT 
    TCATACAATG GCTCTGGAGG TATGGGGAAC AAACAAACCC TGCCAGGGGA GTTGCCAGTG
    AATCAGAGCC CAGTTATAGC AGACATACAG GCAGCATATT GCGAAGGGGA CAAAGAGAGA
    GCACAGAATC TAGTGAAAAG TGCCTGTGAG GAGAATGTCC AGAAGCATGA GAAGTACCAG
    TTTCTGAGAC TAACAGCAGC CTATGGAGAT CTTCCCTCTG TACGTTATCT GCTTAAGGAA
    GCACAGGTAG TTTTACCAAC TGAACCAAAT GATGACAATC CAGCAGTAAT GGCTTCGCAT
    TTTGGCCATG TTGACACAGT AACATTTTTA CTGGATTCTC TGCCAGATCC CTGCAGTCCC
    AAGCCACTGC TGAACTGGAT GCTGGTAACT GCCTGTGAGC AGGGTCACTT GGCTCTGGTT
    AAACTTTTGG TACTAAGATA CAATGCTGAC CCAGACTGTT ATGCTACAAG AAACAATGAG
    TTCCCTGTCC TCGGGAAATT ACCCCTTTAT GCCACCATTA TGGCAGGACA TGAAGAAGTT
    GCTGCCTTTC TGCTTCAAAA TGGAGCTGGC TTCTGTTCGT ACATACTGAT GGACAACCCA
    GAGCTGAGCA AAGTTTTACT GAGAAGAAGT CTTACCACTG AGCCACTAGA ACACAGTCAG
    CAGCAAGTAG CACAGGAGAT GGCTGTTTGT GTGAAGTGGT CTAGCCTTCG GCTGCCCTGG
    GTAGACATTG ACTGGTTTAT TGATATTTCC AACAAGATAA GAGAGCTGGA TATCTCTTCC
    AACTGCCTTG CCTCCCTTCC ATCTGTGGTT CCATGGGGCC TTCTGAACCT GCAGAAACTG
    AACATAAGTG ACAATCAGCT TTGTGACCTG CCCAGTGTAC AGAGCTCTGA GGAAATCATC
    TGCTCAAAGT TACTTGAGGT TGATGTCTCC AATAATAAGC TGACATCACT TCCTTCTGCA
    TTCCTCCACC TGAGCCGGCT CCAGAGACTG TGTGCATCAA AAAACTATAT TGTTGATCTC
    TTCAAAGAAG ATGGTACCAA CTGGATTGGT TTACGGAGGT TACAGGATCT AGACTTGTCT
    GATAATAGGA TCACAGAACT GCCAGCCTCC ATCCTGCACT GCTTCAAATC ACTCAGGAAT
    CTAAATGTAG CTGGAAACAA GCTGAAGGTG TTACCTGAAC CCTGGTCATG TCCACTTAAA
    TATTGCAAGG CTTCAAAAAA TGCCATTGAG GGTCTCCCAG AGAACCTGGC TCTTTTCTGG
    AGGAATCATT TGGTTGAAGT TGATTTTTCT GAAAATGCCA TAAAGGAAGT TCCAAAGTCT
    CTCTTCCAGT TGGAGTGCTT GCAGTCTCTG AAGCTCTCCG GGAATCAACT GACCACACTG
    CCTTCTTCTG ACACCTGGAA CTGCACAAAT CTCAAGTGTC TAGATCTGTC CAAGAACCAG
    CTGGGAAGAA ATGAGGAAGG CACTAGGACC AGACGTATGC CATTTTTTTA CACTAAGAGC
    AGAACAAAGA CAGGGACAGA TCCAGGTTCA GCAGTGGAAT TTCCATTGTT TCTGAAGGAG
    TCACTGGAAG TTCTCTACCT AAATGACAAC CAGTTGGAGT CTGTACCTCC ATCAATCTCT
    CAACTAAAGG GCCTTACAGA GCTCTACTTG GGAAACAATC CAGGTATACG AGAGCTTCCT
    GCAGATTTGG GGCAGTTGTC TAGTCTGTGG CAACTGGACC TTGAGGATCT GAACATCACA
    AACCTGCCCG GGGAGATCAG AAAGGAGGGG CCAGTTACTA TCCTGGCTTA CCTGCGTGCT
    CAGCTGAGAA GGGCAGAACG ATGCCATCTG GTTAAAATGA TAATTGTGGG ACCCCCTCGG
    CAGGGCAAAT CTACCCTTTT TGAGGTTCTG CAGAGTGGGA AAGTGTCTCA GCAAATGCAA
    GGGGACAACT CCATAAGAAC CACCTGCTGG GAGCTACAGA AGCCTGTGGG GACCACTGCA
    AAGGTTGATT CTGTGCAGTT CCATGTGTGG GACGTTGGAG GTTCGAATGC AATGTCCGCT
    GTGAACCAGT GCTTCTTCAC TGACAAAGCC TTGTATGTGG TAGTCTGGAA CCTTGCTCTT
    GGCGAGGAGG CTGTTGCCAA TCTTCAGTTC TGGCTACTGA ACATAGAGGC AAAAGCTCCC
    AATGCCGTTG TTCTGGTTGT AGGGACGCAC CTTGACTTCA TAGAAACCAA ATTCCGGTTG
    GAGAGAATTG CCACTCTTCG AGCTTATGTT CTGGCATTGT GTCGCTCCCC ATCTGGCTCC
    AGAGCAACTG GGTTCCCTGA TATCACTTTC AAGCACTTAC ATGAACTCTC TTGCAAAACA
    TTGGAGGGAC TCGATGGTCT GCGCCAGCTG ATATTCCATG CTGCCTGCAA TATGAGGGAT
    GTAGGCAGTA CCATTGGATC CCAAAAACTA GTGGGAAGGC TGATCCCGAG AAGCTACCTG
    AACCTTCAGG AGGCTGTGGT AGCTGAGCAG CAGAGACGAG CTCAAGGGGA TGAGGTACAA
    TACCTGACTG ATGAGCAGAT AGAACAGATT ATTGAACAGA GCCCTGGAAA CGATATACGC
    GATTATGAAG ACATGCAGGC AGCGATCGGT TTCTTGATAG AAACTGGGAC TTTGCTTCAC
    TTTCCTGACA CTAGCCATGG CCTAAGAAAC CTTTACTTCT TGGACCCTGT TTGGTTGTCA
    GAATGTTTGG AGAGGATTAT TAACATTAAG AGCTCCAAGT CTGTGGCCCG GAATGGGGTG
    ATTAAGGCAG AAGATCTCAG AATGCTGCTG GTTGGAACTG GATTCACCAA ACAGACTGAA
    GAGCAATATT TTCAGTTCCT GGCAAAGTTT GAGATTGCTC TTCCTGTTGC CAATAACAGT
    TACCTTTTGC CACATTTGCT GCCGACAAAG CCCGGCCTGG ACATCCACAG CTTGCGGCAG
    CTTACTGGAA GCACAGTTCA GAGGTTGTTT AAGATGAGCT TTGTTCCTGT TGGGTTCTGG
    CAGCGGTTCA TAGCTCGGAT GTTGATCAGT CTGGCAGAAA TGGATCTCCA GCTGTTTGAA
    AGCAAGAAAA ACACCAAGAC AAAAAACAGG AAATCTGCAA TTTACAGCTT CACTGGGTAT
    CAGAGGAACC GATGTAGCAC CTTCCGAGTG AAGAGGAATC AGACCATTTA CTGGCAGGAA
    GGCCTAATGG TCACCTTTGA AGGGGGTTAC CTCAGTGTTG AGTCATCAGA CATTAACTGG
    AAGAAGAAGA AGAATGGTGG GATTAAAATA ATTTGCCAGT CTGAGCTGAG AGATTTCTCT
    GCTATGGCAT TTATCACAGA CCATGTGAAC TCTCTCATAG ATCAGTGGTT CCCAGCCCTT
    ACAGCCACAG AGAGTGATGG CACTTTACTG ATGGAGCAAT GTGTGCCCTG CCACTGCTGT
    ACCACAGTCG CTAGTGATCA AGAGAAGAAA AATAATGAGA ATACCCCACA TTACTTTAAT
    ATGGAAGACT GCGTCCTAGC AGCTGTTGAG AAGGATTATT TGGAATGCCC GAACCACCCA
    GAACATGCAG TGCCACTGCA GGAACTGGTC CCAGAAATTT TCATGACTGA CTTTCCTTCA
    AGGCTTTTCC TTGACATCAA TGACTTAGAA TACTCACAGG AGAGCTCCAA CATTCTGGGT
    CAGGGAGGAA GTGGCACAGT GATATACCGA GCTGAATATA AGCACAAGCC TGTGGCTGTG
    AAGATGTTTC AGGTTTCTAA GTGTCGGGCA TCTCCAGAGG TCATGCAAGA CACCATGTTC
    AAGCACTTGC AGGCAATGGA CGCCATGAGA AACTTCTCGG AATTCCGTCA GGAGGCCAGC
    ATGCTCCATA CTCTGCAGCA CCCCTGCATT GTCTCCCTGA TTGGCATCAG CATCCATCCA
    CTGTGCTTTG CCCTGGAGCT GGCACCTTTG GGGAGCCTCA ACACAGTGCT GGCAGGAACA
    GCCCAAGATG CAAGTTCATT CATGCCTCTT GGTCACATGC TCACTCAGCG TATTTCCTAT
    CAAATTGCAT CTGGGCTGTC CTACCTGCAT AAAAAGAATA TAATATTTTG TGACCTGAAA
    TCAGATAACA TCCTGGTTTG GTCCCTGGAT GTCAAGGAAG CTGTGATAAT CAAACTCTCA
    GACTATGGCA TTTCGCGGCT GTCATTCCAT GAGGGAGCCC TGGGTGTGGA AGGCACCCCA
    GGATATCAAG CACCCGAAAT CCGTCCTCGG ATTGTGTATG ATGAAAAGGT GGATATGTTT
    TCATATGGTA TGGTCCTGTA TGAGCTATTG TCAGGCCAGA GACCATCTTT GGGTCAGCAC
    CAGCTGCAGA TTTCCAAGAA ACTGTCCAAG GGGATTCGTC CAGTTCTTGG ACGCCCAGAG
    GAAGTTCACT TCCATCATCT GCAGGACCTG ATGATGGAAT GTTGGGACAC GAAGCCTGAA
    AAGCGGCCTT TAGCTTCATC AGTTGTCCAT CGTATGAATG ACCCCAGCTT CACAGTTTTT
    AAGTACCAGT TGTCTTGTGG GATACAGGAA TCATTCTGCT CATCCAGAGA ACCAGAGAGC
    ACAGTTGTAT TCTGGGTAGG AACAGGGACA TCAAGGAATT ATACCGTGGT TGATGTTGAG
    AAAGGGCAGG TGGAAGTGCA GACTCTGAGC TGCCCCGGGA TGAGAATGTC TTGCCAGCAA
    AGGGTACAGA ATACACTTTG GATTGCAACA GAGGATCAGA AGATATCTAT CTTTAGTCTG
    CAGGGCATGT GCCCCCTAAG TGTCGCACTA AAGGTGATAG ACACCCCTGC CATTGTGACC
    TGCTTCCTTG TAGCGAATAT GGAAAAGGGG AACTGCCTGG TATTTGCTGG CCTTTCAGAC
    AGCCTGATTT CAGTGTTCCC AGTAAGTGGT GGCATCCCAG GCAGTGATTG CTCCTACATC
    TGTTCCCGCA CAGCTAATCG CTCCATGTTC AAGATAACAG ATGATGACCC ACGTCAGAAA
    CCTCACCCAG TGTTGGCCAT GGAGATCTGC AATAAATGTT CTGAGGTCTG GTATGGCAAT
    GGACCTGGTG TCCTTGTAGT GGACTGTACC TCTCTTGAGA TTTCAAAAAG ACTGGAACCC
    TTTAGTGCAC CATCATCCAT CATCTCCATG GTTACAAGCA CAGACACTAA CAGAGAGGAG
    ACTGTTTGGT TATTGGATGA TATGACCAAT TTTCTGGTCA TGTATAATGC TGCAGCCTAC
    CAACTCTGTG CCCGATACTT CTGTGGTGAT AGCAGTCCTC TAAGGGATAT GTTTACAGTA
    AAGCCTTGTT CAGATGTGGT TCCTGTTGCA TTGCCAGTTA CAAACTGTAA TAGCCCCCGC
    TCAGCAAACG TCAGCATCAT CTACAGCCTA GAACGTGGTA TGCAAGTTCT CAGCCACCAA
    GACTCTCTTA CCGATTATTG CTCTGTGTCA TCCTTCTATT CTTCCATTCC TCAGGGGACT
    GCTAAGTCCT CCTCCAGTTT GCCAAGCACT CCGGTCAGCT ACAGTAGTGC CCCATTTTCC
    ACAGATAGTG AGGATCCAGA CCCAGCCCAA GAACTTATAC ACAAAATTTC TAGTGAGAGC
    TGGGGGGGCA GAACACCTCT TTCAGAGGAG GAAAAACTCA ATGTGCAGGC TGTTGGGATT
    GTAGTCGTCA AAGATTTGCT CTGGGTTCCG AGGCGCGGAG GGGACATTAT TATCATTGGA
    CTGGAGAAGG AGTGTGGTGG ACAAAGAGGT CGTGTCATTG CTGTGTTACA AACTGGCCTA
    GTAGAAAATC AAGGGGTCCA TCTCCACGCT GCTGCTCTTT GCAAGGATTC GGTTGTGTGC
    TGCTTGCAGA GTGAGACACT AGGGTGCTGC TTGTCCGTCT GGAGGAATTG GGGATCAGAG
    GAGTTTAACA TTTTTTATCA TGCCTCTGAT GAACTTAGAA GGCTGGAAAA CTGCATGAGG
    AAAAGGAGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815032.1
    CDS403..6414
    Translation

    Target ORF information:

    RefSeq Version XM_012959578.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis leucine-rich repeat kinase 1 (lrrk1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959578.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGATGTACT GGTGTGTTGG AGTTGAAGCA TGTGCTGGAA GCAGAGGTGG AAGCATGGAT 
    TCATACAATG GCTCTGGAGG TATGGGGAAC AAACAAACCC TGCCAGGGGA GTTGCCAGTG
    AATCAGAGCC CAGTTATAGC AGACATACAG GCAGCATATT GCGAAGGGGA CAAAGAGAGA
    GCACAGAATC TAGTGAAAAG TGCCTGTGAG GAGAATGTCC AGAAGCATGA GAAGTACCAG
    TTTCTGAGAC TAACAGCAGC CTATGGAGAT CTTCCCTCTG TACGTTATCT GCTTAAGGAA
    GCACAGGTAG TTTTACCAAC TGAACCAAAT GATGACAATC CAGCAGTAAT GGCTTCGCAT
    TTTGGCCATG TTGACACAGT AACATTTTTA CTGGATTCTC TGCCAGATCC CTGCAGTCCC
    AAGCCACTGC TGAACTGGAT GCTGGTAACT GCCTGTGAGC AGGGTCACTT GGCTCTGGTT
    AAACTTTTGG TACTAAGATA CAATGCTGAC CCAGACTGTT ATGCTACAAG AAACAATGAG
    TTCCCTGTCC TCGGGAAATT ACCCCTTTAT GCCACCATTA TGGCAGGACA TGAAGAAGTT
    GCTGCCTTTC TGCTTCAAAA TGGAGCTGGC TTCTGTTCGT ACATACTGAT GGACAACCCA
    GAGCTGAGCA AAGTTTTACT GAGAAGAAGT CTTACCACTG AGCCACTAGA ACACAGTCAG
    CAGCAAGTAG CACAGGAGAT GGCTGTTTGT GTGAAGTGGT CTAGCCTTCG GCTGCCCTGG
    GTAGACATTG ACTGGTTTAT TGATATTTCC AACAAGATAA GAGAGCTGGA TATCTCTTCC
    AACTGCCTTG CCTCCCTTCC ATCTGTGGTT CCATGGGGCC TTCTGAACCT GCAGAAACTG
    AACATAAGTG ACAATCAGCT TTGTGACCTG CCCAGTGTAC AGAGCTCTGA GGAAATCATC
    TGCTCAAAGT TACTTGAGGT TGATGTCTCC AATAATAAGC TGACATCACT TCCTTCTGCA
    TTCCTCCACC TGAGCCGGCT CCAGAGACTG TGTGCATCAA AAAACTATAT TGTTGATCTC
    TTCAAAGAAG ATGGTACCAA CTGGATTGGT TTACGGAGGT TACAGGATCT AGACTTGTCT
    GATAATAGGA TCACAGAACT GCCAGCCTCC ATCCTGCACT GCTTCAAATC ACTCAGGAAT
    CTAAATGTAG CTGGAAACAA GCTGAAGGTG TTACCTGAAC CCTGGTCATG TCCACTTAAA
    TATTGCAAGG CTTCAAAAAA TGCCATTGAG GGTCTCCCAG AGAACCTGGC TCTTTTCTGG
    AGGAATCATT TGGTTGAAGT TGATTTTTCT GAAAATGCCA TAAAGGAAGT TCCAAAGTCT
    CTCTTCCAGT TGGAGTGCTT GCAGTCTCTG AAGCTCTCCG GGAATCAACT GACCACACTG
    CCTTCTTCTG ACACCTGGAA CTGCACAAAT CTCAAGTGTC TAGATCTGTC CAAGAACCAG
    CTGGGAAGAA ATGAGGAAGG CACTAGGACC AGACGTATGC CATTTTTTTA CACTAAGAGC
    AGAACAAAGA CAGGGACAGA TCCAGGTTCA GCAGTGGAAT TTCCATTGTT TCTGAAGGAG
    TCACTGGAAG TTCTCTACCT AAATGACAAC CAGTTGGAGT CTGTACCTCC ATCAATCTCT
    CAACTAAAGG GCCTTACAGA GCTCTACTTG GGAAACAATC CAGGTATACG AGAGCTTCCT
    GCAGATTTGG GGCAGTTGTC TAGTCTGTGG CAACTGGACC TTGAGGATCT GAACATCACA
    AACCTGCCCG GGGAGATCAG AAAGGAGGGG CCAGTTACTA TCCTGGCTTA CCTGCGTGCT
    CAGCTGAGAA GGGCAGAACG ATGCCATCTG GTTAAAATGA TAATTGTGGG ACCCCCTCGG
    CAGGGCAAAT CTACCCTTTT TGAGGTTCTG CAGAGTGGGA AAGTGTCTCA GCAAATGCAA
    GGGGACAACT CCATAAGAAC CACCTGCTGG GAGCTACAGA AGCCTGTGGG GACCACTGCA
    AAGGTTGATT CTGTGCAGTT CCATGTGTGG GACGTTGGAG GTTCGAATGC AATGTCCGCT
    GTGAACCAGT GCTTCTTCAC TGACAAAGCC TTGTATGTGG TAGTCTGGAA CCTTGCTCTT
    GGCGAGGAGG CTGTTGCCAA TCTTCAGTTC TGGCTACTGA ACATAGAGGC AAAAGCTCCC
    AATGCCGTTG TTCTGGTTGT AGGGACGCAC CTTGACTTCA TAGAAACCAA ATTCCGGTTG
    GAGAGAATTG CCACTCTTCG AGCTTATGTT CTGGCATTGT GTCGCTCCCC ATCTGGCTCC
    AGAGCAACTG GGTTCCCTGA TATCACTTTC AAGCACTTAC ATGAACTCTC TTGCAAAACA
    TTGGAGGGAC TCGATGGTCT GCGCCAGCTG ATATTCCATG CTGCCTGCAA TATGAGGGAT
    GTAGGCAGTA CCATTGGATC CCAAAAACTA GTGGGAAGGC TGATCCCGAG AAGCTACCTG
    AACCTTCAGG AGGCTGTGGT AGCTGAGCAG CAGAGACGAG CTCAAGGGGA TGAGGTACAA
    TACCTGACTG ATGAGCAGAT AGAACAGATT ATTGAACAGA GCCCTGGAAA CGATATACGC
    GATTATGAAG ACATGCAGGC AGCGATCGGT TTCTTGATAG AAACTGGGAC TTTGCTTCAC
    TTTCCTGACA CTAGCCATGG CCTAAGAAAC CTTTACTTCT TGGACCCTGT TTGGTTGTCA
    GAATGTTTGG AGAGGATTAT TAACATTAAG AGCTCCAAGT CTGTGGCCCG GAATGGGGTG
    ATTAAGGCAG AAGATCTCAG AATGCTGCTG GTTGGAACTG GATTCACCAA ACAGACTGAA
    GAGCAATATT TTCAGTTCCT GGCAAAGTTT GAGATTGCTC TTCCTGTTGC CAATAACAGT
    TACCTTTTGC CACATTTGCT GCCGACAAAG CCCGGCCTGG ACATCCACAG CTTGCGGCAG
    CTTACTGGAA GCACAGTTCA GAGGTTGTTT AAGATGAGCT TTGTTCCTGT TGGGTTCTGG
    CAGCGGTTCA TAGCTCGGAT GTTGATCAGT CTGGCAGAAA TGGATCTCCA GCTGTTTGAA
    AGCAAGAAAA ACACCAAGAC AAAAAACAGG AAATCTGCAA TTTACAGCTT CACTGGGTAT
    CAGAGGAACC GATGTAGCAC CTTCCGAGTG AAGAGGAATC AGACCATTTA CTGGCAGGAA
    GGCCTAATGG TCACCTTTGA AGGGGGTTAC CTCAGTGTTG AGTCATCAGA CATTAACTGG
    AAGAAGAAGA AGAATGGTGG GATTAAAATA ATTTGCCAGT CTGAGCTGAG AGATTTCTCT
    GCTATGGCAT TTATCACAGA CCATGTGAAC TCTCTCATAG ATCAGTGGTT CCCAGCCCTT
    ACAGCCACAG AGAGTGATGG CACTTTACTG ATGGAGCAAT GTGTGCCCTG CCACTGCTGT
    ACCACAGTCG CTAGTGATCA AGAGAAGAAA AATAATGAGA ATACCCCACA TTACTTTAAT
    ATGGAAGACT GCGTCCTAGC AGCTGTTGAG AAGGATTATT TGGAATGCCC GAACCACCCA
    GAACATGCAG TGCCACTGCA GGAACTGGTC CCAGAAATTT TCATGACTGA CTTTCCTTCA
    AGGCTTTTCC TTGACATCAA TGACTTAGAA TACTCACAGG AGAGCTCCAA CATTCTGGGT
    CAGGGAGGAA GTGGCACAGT GATATACCGA GCTGAATATA AGCACAAGCC TGTGGCTGTG
    AAGATGTTTC AGGTTTCTAA GTGTCGGGCA TCTCCAGAGG TCATGCAAGA CACCATGTTC
    AAGCACTTGC AGGCAATGGA CGCCATGAGA AACTTCTCGG AATTCCGTCA GGAGGCCAGC
    ATGCTCCATA CTCTGCAGCA CCCCTGCATT GTCTCCCTGA TTGGCATCAG CATCCATCCA
    CTGTGCTTTG CCCTGGAGCT GGCACCTTTG GGGAGCCTCA ACACAGTGCT GGCAGGAACA
    GCCCAAGATG CAAGTTCATT CATGCCTCTT GGTCACATGC TCACTCAGCG TATTTCCTAT
    CAAATTGCAT CTGGGCTGTC CTACCTGCAT AAAAAGAATA TAATATTTTG TGACCTGAAA
    TCAGATAACA TCCTGGTTTG GTCCCTGGAT GTCAAGGAAG CTGTGATAAT CAAACTCTCA
    GACTATGGCA TTTCGCGGCT GTCATTCCAT GAGGGAGCCC TGGGTGTGGA AGGCACCCCA
    GGATATCAAG CACCCGAAAT CCGTCCTCGG ATTGTGTATG ATGAAAAGGT GGATATGTTT
    TCATATGGTA TGGTCCTGTA TGAGCTATTG TCAGGCCAGA GACCATCTTT GGGTCAGCAC
    CAGCTGCAGA TTTCCAAGAA ACTGTCCAAG GGGATTCGTC CAGTTCTTGG ACGCCCAGAG
    GAAGTTCACT TCCATCATCT GCAGGACCTG ATGATGGAAT GTTGGGACAC GAAGCCTGAA
    AAGCGGCCTT TAGCTTCATC AGTTGTCCAT CGTATGAATG ACCCCAGCTT CACAGTTTTT
    AAGTACCAGT TGTCTTGTGG GATACAGGAA TCATTCTGCT CATCCAGAGA ACCAGAGAGC
    ACAGTTGTAT TCTGGGTAGG AACAGGGACA TCAAGGAATT ATACCGTGGT TGATGTTGAG
    AAAGGGCAGG TGGAAGTGCA GACTCTGAGC TGCCCCGGGA TGAGAATGTC TTGCCAGCAA
    AGGGTACAGA ATACACTTTG GATTGCAACA GAGGATCAGA AGATATCTAT CTTTAGTCTG
    CAGGGCATGT GCCCCCTAAG TGTCGCACTA AAGGTGATAG ACACCCCTGC CATTGTGACC
    TGCTTCCTTG TAGCGAATAT GGAAAAGGGG AACTGCCTGG TATTTGCTGG CCTTTCAGAC
    AGCCTGATTT CAGTGTTCCC AGTAAGTGGT GGCATCCCAG GCAGTGATTG CTCCTACATC
    TGTTCCCGCA CAGCTAATCG CTCCATGTTC AAGATAACAG ATGATGACCC ACGTCAGAAA
    CCTCACCCAG TGTTGGCCAT GGAGATCTGC AATAAATGTT CTGAGGTCTG GTATGGCAAT
    GGACCTGGTG TCCTTGTAGT GGACTGTACC TCTCTTGAGA TTTCAAAAAG ACTGGAACCC
    TTTAGTGCAC CATCATCCAT CATCTCCATG GTTACAAGCA CAGACACTAA CAGAGAGGAG
    ACTGTTTGGT TATTGGATGA TATGACCAAT TTTCTGGTCA TGTATAATGC TGCAGCCTAC
    CAACTCTGTG CCCGATACTT CTGTGGTGAT AGCAGTCCTC TAAGGGATAT GTTTACAGTA
    AAGCCTTGTT CAGATGTGGT TCCTGTTGCA TTGCCAGTTA CAAACTGTAA TAGCCCCCGC
    TCAGCAAACG TCAGCATCAT CTACAGCCTA GAACGTGGTA TGCAAGTTCT CAGCCACCAA
    GACTCTCTTA CCGATTATTG CTCTGTGTCA TCCTTCTATT CTTCCATTCC TCAGGGGACT
    GCTAAGTCCT CCTCCAGTTT GCCAAGCACT CCGGTCAGCT ACAGTAGTGC CCCATTTTCC
    ACAGATAGTG AGGATCCAGA CCCAGCCCAA GAACTTATAC ACAAAATTTC TAGTGAGAGC
    TGGGGGGGCA GAACACCTCT TTCAGAGGAG GAAAAACTCA ATGTGCAGGC TGTTGGGATT
    GTAGTCGTCA AAGATTTGCT CTGGGTTCCG AGGCGCGGAG GGGACATTAT TATCATTGGA
    CTGGAGAAGG AGTGTGGTGG ACAAAGAGGT CGTGTCATTG CTGTGTTACA AACTGGCCTA
    GTAGAAAATC AAGGGGTCCA TCTCCACGCT GCTGCTCTTT GCAAGGATTC GGTTGTGTGC
    TGCTTGCAGA GTGAGACACT AGGGTGCTGC TTGTCCGTCT GGAGGAATTG GGGATCAGAG
    GAGTTTAACA TTTTTTATCA TGCCTCTGAT GAACTTAGAA GGCTGGAAAA CTGCATGAGG
    AAAAGGAGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.