thra.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	thra.L
Entrez Gene ID	397942
Full Name	thyroid hormone receptor, alpha L homeolog
Synonyms	TRalpha,c-erba,erba,nr1a1-A,thra,thra-A,thra1
General protein information	Preferred Names thyroid hormone receptor, alpha L homeolog Names thyroid hormone receptor alpha-A TRalphaA nuclear receptor subfamily 1 group A member 1-A thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog) xenTR-alpha1
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018234144.1	XP_018089633.1	thyroid hormone receptor alpha-A isoform X1
XM_018234145.1	XP_018089634.1	thyroid hormone receptor alpha-A isoform X2
NM_001088126.1	NP_001081595.1	thyroid hormone receptor alpha-A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Regulation of growth rate and developmental timing by Xenopus thyroid hormone receptor ?.
Wen L, Shi YB
Development, growth & differentiation58(1)106-15(2016 Jan)

Deciphering the regulatory logic of an ancient, ultraconserved nuclear receptor enhancer module.
Bagamasbad PD, Bonett RM, Sachs L, Buisine N, Raj S, Knoedler JR, Kyono Y, Ruan Y, Ruan X, Denver RJ
Molecular endocrinology (Baltimore, Md.)29(6)856-72(2015 Jun)

Regulation of thyroid hormone sensitivity by differential expression of the thyroid hormone receptor during Xenopus metamorphosis.
Nakajima K, Fujimoto K, Yaoita Y
Genes to cells : devoted to molecular & cellular mechanisms17(8)645-59(2012 Aug)

Thyroid hormone receptor subtype specificity for hormone-dependent neurogenesis in Xenopus laevis.
Denver RJ, Hu F, Scanlan TS, Furlow JD
Developmental biology326(1)155-68(2009 Feb)

Novel functions of protein arginine methyltransferase 1 in thyroid hormone receptor-mediated transcription and in the regulation of metamorphic rate in Xenopus laevis.
Matsuda H, Paul BD, Choi CY, Hasebe T, Shi YB
Molecular and cellular biology29(3)745-57(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Furthermore, molecular and transgenic studies have shown that unliganded TRalpha accomplishes these via the recruitment of histone deacetylase (HDAC)-containing corepressor complexes to repress the expression of TH-inducible genes
Title: Regulation of growth rate and developmental timing by Xenopus thyroid hormone receptor ?.

regions of the Xenopus and mouse Klf9 genes 5-6 kb upstream of the transcription start sites that supported synergistic transactivation by TH plus GC.
Title: Deciphering the regulatory logic of an ancient, ultraconserved nuclear receptor enhancer module.

Type 2 iodothyronine deiodinase activity is required for the death of thyroid hormone receptor (TRalpha)-transfected tail muscle cells induced by a low level of thyroid hormone.
Title: Regulation of thyroid hormone sensitivity by differential expression of the thyroid hormone receptor during Xenopus metamorphosis.

T(3) acts to induce cell proliferation in the tadpole brain predominantly, if not exclusively, via TRalpha.
Title: Thyroid hormone receptor subtype specificity for hormone-dependent neurogenesis in Xenopus laevis.

PRMT1 functions transiently as a coactivator in thyroid hormone (T3) receptor (TR)-mediated transcription by enhancing TR-T3 response element binding and further suggest that PRMT1 has tissue-specific roles in regulating the rate of metamorphosis.
Title: Novel functions of protein arginine methyltransferase 1 in thyroid hormone receptor-mediated transcription and in the regulation of metamorphic rate in Xenopus laevis.

The following thra.L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the thra.L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAf00909	NM_001088126.1 Latest version!	Xenopus laevis thyroid hormone receptor alpha L homeolog (thra.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OAf45032	XM_018234144.1 Latest version!	Xenopus laevis thyroid hormone receptor, alpha L homeolog (thra.L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OAf45033	XM_018234145.1 Latest version!	Xenopus laevis thyroid hormone receptor, alpha L homeolog (thra.L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

thra.L ( NM_001088126.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001081595.1 Xenopus laevis thyroid hormone receptor alpha L homeolog (thra.L), mRNA.

Price & Availability↑

CloneID	OAf00909
Clone ID Related Accession (Same CDS sequence)	NM_001088126.1
Accession Version	NM_001088126.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Xenopus laevis(African clawed frog)
Product	thyroid hormone receptor alpha-A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X17385.1. On Jul 25, 2007 this sequence version replaced NM_001088127.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X17385.1 [ECO:0000332] ##Evidence-Data-END##

ATGGACCAGA ATCTCAGCGG GCTGGACTGC TTGTCAGAGC CAGATGAAAA AAGGTGGCCG 
GATGGGAAGC GAAAAAGAAA GAACAGCCAA TGTATGGGAA AAAGCGGCAT GTCCGGTGAC 
AGCTTGGTGT CTCTGCCCTC TGCAGGGTAC ATCCCCAGCC ATCTGGACAA AGATGAGCCA 
TGCGTGGTGT GCAGTGATAA GGCCACGGGG TACCACTACC GCTGTATCAC TTGCGAGGGG 
TGTAAGGGTT TCTTTCGCCG CACCATCCAG AAGAACCTGC ACCCCTCCTA CTCGTGCAAG 
TACGATGGCT GCTGCATTAT CGACAAGATC ACCCGAAATC AGTGCCAGCT CTGCCGCTTC 
AAGAAATGCA TTGCCGTTGG CATGGCAATG GATCTTGTCC TGGATGATAG CAAGCGGGTA 
GCCAAGCGAA AACTGATTGA AGAGAATCGA CAGCGGCGGC GGAAGGAGGA GATGATCAAG 
ACTCTGCAAC AGCGTCCCGA GCCAAGCAGC GAGGAGTGGG AGTTGATTCG CATTGTAACA 
GAAGCTCACA GGAGTACCAA TGCTCAGGGC AGCCACTGGA AACAGCGTAG GAAGTTTCTG 
CCGGAAGATA TCGGGCAGTC TCCCATGGCT TCCATGCCGG ATGGGGATAA AGTTGACCTG 
GAAGCTTTCA GTGAGTTCAC CAAGATAATC ACCCCGGCAA TTACCAGAGT GGTGGACTTT 
GCCAAGAAGC TGCCCATGTT CTCTGAGCTG ACTTGTGAAG ACCAGATCAT CCTGTTGAAA 
GGATGTTGTA TGGAGATCAT GTCTCTCCGT GCTGCTGTAC GCTACGATCC AGACAGCGAG 
ACCCTAACGC TGAGCGGAGA GATGGCTGTG AAACGGGAGC AGCTTAAGAA CGGAGGTCTG 
GGTGTTGTCT CTGATGCCAT CTTTGACCTC GGGAGGTCGC TTGCTGCGTT TAACCTTGAC 
GATACGGAAG TGGCACTGCT GCAGGCTGTT TTGCTAATGT CATCAGACCG AACTGGTTTA 
ATCTGCACGG ACAAGATAGA GAAATGTCAA GAGACCTACC TTCTCGCCTT TGAACACTAC 
ATCAACCATC GCAAACACAA CATTCCCCAC TTCTGGCCCA AACTCCTAAT GAAGGTGACG 
GACCTGCGCA TGATAGGGGC ATGCCATGCC AGCCGCTTTC TGCACATGAA GGTCGAGTGC 
CCCACCGAGC TCTTTCCACC GCTCTTCCTT GAGGTCTTTG AGGACCAGGA AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001081595.1
CDS	460..1716
Translation	MDQNLSGLDCLSEPDEKRWPDGKRKRKNSQCMGKSGMSGDSLVSLPSAGYIPSHLDKDEPCVVCSDKATGYHYRCITCEGCKGFFRRTIQKNLHPSYSCKYDGCCIIDKITRNQCQLCRFKKCIAVGMAMDLVLDDSKRVAKRKLIEENRQRRRKEEMIKTLQQRPEPSSEEWELIRIVTEAHRSTNAQGSHWKQRRKFLPEDIGQSPMASMPDGDKVDLEAFSEFTKIITPAITRVVDFAKKLPMFSELTCEDQIILLKGCCMEIMSLRAAVRYDPDSETLTLSGEMAVKREQLKNGGLGVVSDAIFDLGRSLAAFNLDDTEVALLQAVLLMSSDRTGLICTDKIEKCQETYLLAFEHYINHRKHNIPHFWPKLLMKVTDLRMIGACHASRFLHMKVECPTELFPPLFLEVFEDQEV

Target ORF information:

RefSeq Version	NM_001088126.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis thyroid hormone receptor alpha L homeolog (thra.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001088126.1

ORF Insert Sequence:

ATGGACCAGA ATCTCAGCGG GCTGGACTGC TTGTCAGAGC CAGATGAAAA AAGGTGGCCG 
GATGGGAAGC GAAAAAGAAA GAACAGCCAA TGTATGGGAA AAAGCGGCAT GTCCGGTGAC 
AGCTTGGTGT CTCTGCCCTC TGCAGGGTAC ATCCCCAGCC ATCTGGACAA AGATGAGCCA 
TGCGTGGTGT GCAGTGATAA GGCCACGGGG TACCACTACC GCTGTATCAC TTGCGAGGGG 
TGTAAGGGTT TCTTTCGCCG CACCATCCAG AAGAACCTGC ACCCCTCCTA CTCGTGCAAG 
TACGATGGCT GCTGCATTAT CGACAAGATC ACCCGAAATC AGTGCCAGCT CTGCCGCTTC 
AAGAAATGCA TTGCCGTTGG CATGGCAATG GATCTTGTCC TGGATGATAG CAAGCGGGTA 
GCCAAGCGAA AACTGATTGA AGAGAATCGA CAGCGGCGGC GGAAGGAGGA GATGATCAAG 
ACTCTGCAAC AGCGTCCCGA GCCAAGCAGC GAGGAGTGGG AGTTGATTCG CATTGTAACA 
GAAGCTCACA GGAGTACCAA TGCTCAGGGC AGCCACTGGA AACAGCGTAG GAAGTTTCTG 
CCGGAAGATA TCGGGCAGTC TCCCATGGCT TCCATGCCGG ATGGGGATAA AGTTGACCTG 
GAAGCTTTCA GTGAGTTCAC CAAGATAATC ACCCCGGCAA TTACCAGAGT GGTGGACTTT 
GCCAAGAAGC TGCCCATGTT CTCTGAGCTG ACTTGTGAAG ACCAGATCAT CCTGTTGAAA 
GGATGTTGTA TGGAGATCAT GTCTCTCCGT GCTGCTGTAC GCTACGATCC AGACAGCGAG 
ACCCTAACGC TGAGCGGAGA GATGGCTGTG AAACGGGAGC AGCTTAAGAA CGGAGGTCTG 
GGTGTTGTCT CTGATGCCAT CTTTGACCTC GGGAGGTCGC TTGCTGCGTT TAACCTTGAC 
GATACGGAAG TGGCACTGCT GCAGGCTGTT TTGCTAATGT CATCAGACCG AACTGGTTTA 
ATCTGCACGG ACAAGATAGA GAAATGTCAA GAGACCTACC TTCTCGCCTT TGAACACTAC 
ATCAACCATC GCAAACACAA CATTCCCCAC TTCTGGCCCA AACTCCTAAT GAAGGTGACG 
GACCTGCGCA TGATAGGGGC ATGCCATGCC AGCCGCTTTC TGCACATGAA GGTCGAGTGC 
CCCACCGAGC TCTTTCCACC GCTCTTCCTT GAGGTCTTTG AGGACCAGGA AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

thra.L ( XM_018234144.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018089633.1 Xenopus laevis thyroid hormone receptor, alpha L homeolog (thra.L), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAf45032
Clone ID Related Accession (Same CDS sequence)	XM_018234144.1
Accession Version	XM_018234144.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-19
Organism	Xenopus laevis(African clawed frog)
Product	thyroid hormone receptor alpha-A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030740.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCAGA ATCTCAGCGG GCTGGACTGC TTGTCAGAGC CAGATGAAAA AAGGTGGCCG 
GATGGGAAGC GAAAAAGAAA GAACAGCCAA TGTATGGGAA AAAGCGGCAT GTCCGGTGAC 
AGCTTGGTGT CTCTGCCCTC TGCAGGGTAC ATCCCCAGCT ATCTGGACAA AGATGAGCCA 
TGCGTGGTGT GCAGTGATAA GGCCACGGGG TACCACTACC GCTGTATCAC TTGCGAGGGG 
TGTAAGGGTT TCTTTCGCCG CACCATCCAG AAGAACCTGC ACCCCTCCTA CTCGTGCAAG 
TACGATGGCT GCTGCATTAT CGACAAGATC ACCCGAAATC AGTGCCAGCT CTGCCGCTTC 
AAGAAATGCA TTGCCGTTGG CATGGCAATG GATCTTGTCC TGGATGATAG CAAGCGGGTA 
GCCAAGCGAA AACTGATTGA AGAGAATCGA CAGCGGCGGC GGAAGGAGGA GATGATCAAG 
ACTCTGCAAC AGCGTCCCGA GCCAAGCAGC GAGGAGTGGG AGTTGATTCG CATTGTAACA 
GAAGCTCACA GGAGTACCAA TGCTCAGGGC AGCCACTGGA AACAGCGTAG GAAGTTTCTG 
CCGGAAGATA TCGGGCAGTC TCCCATGGCT TCCATGCCGG ATGGGGATAA AGTTGACCTG 
GAAGCTTTCA GTGAGTTCAC CAAGATAATC ACCCCGGCAA TTACCAGAGT GGTGGACTTT 
GCCAAGAAGC TGCCCATGTT CTCTGAGCTG ACTTGTGAAG ACCAGATCAT CCTGTTGAAA 
GGATGTTGTA TGGAGATCAT GTCTCTCCGT GCTGCTGTAC GCTACGATCC AGACAGCGAG 
ACCCTAACGC TGAGCGGAGA GATGGCTGTG AAACGGGAGC AGCTTAAGAA CGGAGGTCTG 
GGTGTTGTCT CTGATGCCAT CTTTGACCTC GGGAGGTCGC TTGCTGCGTT TAACCTTGAC 
GATACGGAAG TGGCACTGCT GCAGGCTGTT TTGCTAATGT CATCAGACCG AACTGGTTTA 
ATCTGCACGG ACAAGATAGA GAAATGTCAA GAGACCTACC TTCTCGCCTT TGAACACTAC 
ATCAACCATC GCAAACACAA CATTCCCCAC TTCTGGCCCA AACTCCTAAT GAAGGTGACG 
GACCTGCGCA TGATAGGGGC ATGCCATGCC AGCCGCTTTC TGCACATGAA GGTCGAGTGC 
CCCACCGAGC TCTTTCCACC GCTCTTCCTT GAGGTCTTTG AGGACCAGGA AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018089633.1
CDS	602..1858
Translation	MDQNLSGLDCLSEPDEKRWPDGKRKRKNSQCMGKSGMSGDSLVSLPSAGYIPSYLDKDEPCVVCSDKATGYHYRCITCEGCKGFFRRTIQKNLHPSYSCKYDGCCIIDKITRNQCQLCRFKKCIAVGMAMDLVLDDSKRVAKRKLIEENRQRRRKEEMIKTLQQRPEPSSEEWELIRIVTEAHRSTNAQGSHWKQRRKFLPEDIGQSPMASMPDGDKVDLEAFSEFTKIITPAITRVVDFAKKLPMFSELTCEDQIILLKGCCMEIMSLRAAVRYDPDSETLTLSGEMAVKREQLKNGGLGVVSDAIFDLGRSLAAFNLDDTEVALLQAVLLMSSDRTGLICTDKIEKCQETYLLAFEHYINHRKHNIPHFWPKLLMKVTDLRMIGACHASRFLHMKVECPTELFPPLFLEVFEDQEV

Target ORF information:

RefSeq Version	XM_018234144.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis thyroid hormone receptor, alpha L homeolog (thra.L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018234144.1

ORF Insert Sequence:

ATGGACCAGA ATCTCAGCGG GCTGGACTGC TTGTCAGAGC CAGATGAAAA AAGGTGGCCG 
GATGGGAAGC GAAAAAGAAA GAACAGCCAA TGTATGGGAA AAAGCGGCAT GTCCGGTGAC 
AGCTTGGTGT CTCTGCCCTC TGCAGGGTAC ATCCCCAGCT ATCTGGACAA AGATGAGCCA 
TGCGTGGTGT GCAGTGATAA GGCCACGGGG TACCACTACC GCTGTATCAC TTGCGAGGGG 
TGTAAGGGTT TCTTTCGCCG CACCATCCAG AAGAACCTGC ACCCCTCCTA CTCGTGCAAG 
TACGATGGCT GCTGCATTAT CGACAAGATC ACCCGAAATC AGTGCCAGCT CTGCCGCTTC 
AAGAAATGCA TTGCCGTTGG CATGGCAATG GATCTTGTCC TGGATGATAG CAAGCGGGTA 
GCCAAGCGAA AACTGATTGA AGAGAATCGA CAGCGGCGGC GGAAGGAGGA GATGATCAAG 
ACTCTGCAAC AGCGTCCCGA GCCAAGCAGC GAGGAGTGGG AGTTGATTCG CATTGTAACA 
GAAGCTCACA GGAGTACCAA TGCTCAGGGC AGCCACTGGA AACAGCGTAG GAAGTTTCTG 
CCGGAAGATA TCGGGCAGTC TCCCATGGCT TCCATGCCGG ATGGGGATAA AGTTGACCTG 
GAAGCTTTCA GTGAGTTCAC CAAGATAATC ACCCCGGCAA TTACCAGAGT GGTGGACTTT 
GCCAAGAAGC TGCCCATGTT CTCTGAGCTG ACTTGTGAAG ACCAGATCAT CCTGTTGAAA 
GGATGTTGTA TGGAGATCAT GTCTCTCCGT GCTGCTGTAC GCTACGATCC AGACAGCGAG 
ACCCTAACGC TGAGCGGAGA GATGGCTGTG AAACGGGAGC AGCTTAAGAA CGGAGGTCTG 
GGTGTTGTCT CTGATGCCAT CTTTGACCTC GGGAGGTCGC TTGCTGCGTT TAACCTTGAC 
GATACGGAAG TGGCACTGCT GCAGGCTGTT TTGCTAATGT CATCAGACCG AACTGGTTTA 
ATCTGCACGG ACAAGATAGA GAAATGTCAA GAGACCTACC TTCTCGCCTT TGAACACTAC 
ATCAACCATC GCAAACACAA CATTCCCCAC TTCTGGCCCA AACTCCTAAT GAAGGTGACG 
GACCTGCGCA TGATAGGGGC ATGCCATGCC AGCCGCTTTC TGCACATGAA GGTCGAGTGC 
CCCACCGAGC TCTTTCCACC GCTCTTCCTT GAGGTCTTTG AGGACCAGGA AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

thra.L ( XM_018234145.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018089634.1 Xenopus laevis thyroid hormone receptor, alpha L homeolog (thra.L), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAf45033
Clone ID Related Accession (Same CDS sequence)	XM_018234145.1
Accession Version	XM_018234145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-19
Organism	Xenopus laevis(African clawed frog)
Product	thyroid hormone receptor alpha-A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030740.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCAGA ATCTCAGCGG GCTGGACTGC TTGTCAGAGC CAGATGAAAA AAGGTGGCCG 
GATGGGAAGC GAAAAAGAAA GAACAGCCAA TGTATGGGAA AAAGCGGCAT GTCCGGGTAC 
ATCCCCAGCT ATCTGGACAA AGATGAGCCA TGCGTGGTGT GCAGTGATAA GGCCACGGGG 
TACCACTACC GCTGTATCAC TTGCGAGGGG TGTAAGGGTT TCTTTCGCCG CACCATCCAG 
AAGAACCTGC ACCCCTCCTA CTCGTGCAAG TACGATGGCT GCTGCATTAT CGACAAGATC 
ACCCGAAATC AGTGCCAGCT CTGCCGCTTC AAGAAATGCA TTGCCGTTGG CATGGCAATG 
GATCTTGTCC TGGATGATAG CAAGCGGGTA GCCAAGCGAA AACTGATTGA AGAGAATCGA 
CAGCGGCGGC GGAAGGAGGA GATGATCAAG ACTCTGCAAC AGCGTCCCGA GCCAAGCAGC 
GAGGAGTGGG AGTTGATTCG CATTGTAACA GAAGCTCACA GGAGTACCAA TGCTCAGGGC 
AGCCACTGGA AACAGCGTAG GAAGTTTCTG CCGGAAGATA TCGGGCAGTC TCCCATGGCT 
TCCATGCCGG ATGGGGATAA AGTTGACCTG GAAGCTTTCA GTGAGTTCAC CAAGATAATC 
ACCCCGGCAA TTACCAGAGT GGTGGACTTT GCCAAGAAGC TGCCCATGTT CTCTGAGCTG 
ACTTGTGAAG ACCAGATCAT CCTGTTGAAA GGATGTTGTA TGGAGATCAT GTCTCTCCGT 
GCTGCTGTAC GCTACGATCC AGACAGCGAG ACCCTAACGC TGAGCGGAGA GATGGCTGTG 
AAACGGGAGC AGCTTAAGAA CGGAGGTCTG GGTGTTGTCT CTGATGCCAT CTTTGACCTC 
GGGAGGTCGC TTGCTGCGTT TAACCTTGAC GATACGGAAG TGGCACTGCT GCAGGCTGTT 
TTGCTAATGT CATCAGACCG AACTGGTTTA ATCTGCACGG ACAAGATAGA GAAATGTCAA 
GAGACCTACC TTCTCGCCTT TGAACACTAC ATCAACCATC GCAAACACAA CATTCCCCAC 
TTCTGGCCCA AACTCCTAAT GAAGGTGACG GACCTGCGCA TGATAGGGGC ATGCCATGCC 
AGCCGCTTTC TGCACATGAA GGTCGAGTGC CCCACCGAGC TCTTTCCACC GCTCTTCCTT 
GAGGTCTTTG AGGACCAGGA AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018089634.1
CDS	603..1829
Translation	MDQNLSGLDCLSEPDEKRWPDGKRKRKNSQCMGKSGMSGYIPSYLDKDEPCVVCSDKATGYHYRCITCEGCKGFFRRTIQKNLHPSYSCKYDGCCIIDKITRNQCQLCRFKKCIAVGMAMDLVLDDSKRVAKRKLIEENRQRRRKEEMIKTLQQRPEPSSEEWELIRIVTEAHRSTNAQGSHWKQRRKFLPEDIGQSPMASMPDGDKVDLEAFSEFTKIITPAITRVVDFAKKLPMFSELTCEDQIILLKGCCMEIMSLRAAVRYDPDSETLTLSGEMAVKREQLKNGGLGVVSDAIFDLGRSLAAFNLDDTEVALLQAVLLMSSDRTGLICTDKIEKCQETYLLAFEHYINHRKHNIPHFWPKLLMKVTDLRMIGACHASRFLHMKVECPTELFPPLFLEVFEDQEV

Target ORF information:

RefSeq Version	XM_018234145.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis thyroid hormone receptor, alpha L homeolog (thra.L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018234145.1

ORF Insert Sequence:

ATGGACCAGA ATCTCAGCGG GCTGGACTGC TTGTCAGAGC CAGATGAAAA AAGGTGGCCG 
GATGGGAAGC GAAAAAGAAA GAACAGCCAA TGTATGGGAA AAAGCGGCAT GTCCGGGTAC 
ATCCCCAGCT ATCTGGACAA AGATGAGCCA TGCGTGGTGT GCAGTGATAA GGCCACGGGG 
TACCACTACC GCTGTATCAC TTGCGAGGGG TGTAAGGGTT TCTTTCGCCG CACCATCCAG 
AAGAACCTGC ACCCCTCCTA CTCGTGCAAG TACGATGGCT GCTGCATTAT CGACAAGATC 
ACCCGAAATC AGTGCCAGCT CTGCCGCTTC AAGAAATGCA TTGCCGTTGG CATGGCAATG 
GATCTTGTCC TGGATGATAG CAAGCGGGTA GCCAAGCGAA AACTGATTGA AGAGAATCGA 
CAGCGGCGGC GGAAGGAGGA GATGATCAAG ACTCTGCAAC AGCGTCCCGA GCCAAGCAGC 
GAGGAGTGGG AGTTGATTCG CATTGTAACA GAAGCTCACA GGAGTACCAA TGCTCAGGGC 
AGCCACTGGA AACAGCGTAG GAAGTTTCTG CCGGAAGATA TCGGGCAGTC TCCCATGGCT 
TCCATGCCGG ATGGGGATAA AGTTGACCTG GAAGCTTTCA GTGAGTTCAC CAAGATAATC 
ACCCCGGCAA TTACCAGAGT GGTGGACTTT GCCAAGAAGC TGCCCATGTT CTCTGAGCTG 
ACTTGTGAAG ACCAGATCAT CCTGTTGAAA GGATGTTGTA TGGAGATCAT GTCTCTCCGT 
GCTGCTGTAC GCTACGATCC AGACAGCGAG ACCCTAACGC TGAGCGGAGA GATGGCTGTG 
AAACGGGAGC AGCTTAAGAA CGGAGGTCTG GGTGTTGTCT CTGATGCCAT CTTTGACCTC 
GGGAGGTCGC TTGCTGCGTT TAACCTTGAC GATACGGAAG TGGCACTGCT GCAGGCTGTT 
TTGCTAATGT CATCAGACCG AACTGGTTTA ATCTGCACGG ACAAGATAGA GAAATGTCAA 
GAGACCTACC TTCTCGCCTT TGAACACTAC ATCAACCATC GCAAACACAA CATTCCCCAC 
TTCTGGCCCA AACTCCTAAT GAAGGTGACG GACCTGCGCA TGATAGGGGC ATGCCATGCC 
AGCCGCTTTC TGCACATGAA GGTCGAGTGC CCCACCGAGC TCTTTCCACC GCTCTTCCTT 
GAGGTCTTTG AGGACCAGGA AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of growth rate and developmental timing by Xenopus thyroid hormone receptor ?.
Development, growth & differentiation58(1)106-15(2016 Jan)
Wen L,Shi YB

Deciphering the regulatory logic of an ancient, ultraconserved nuclear receptor enhancer module.
Molecular endocrinology (Baltimore, Md.)29(6)856-72(2015 Jun)
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