LOC102076273 cDNA ORF clone, Oreochromis niloticus(Nile tilapia)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC102076273
Entrez Gene ID	102076273
Full Name	protein NLRC3
Gene Type	protein-coding
Organism	Oreochromis niloticus(Nile tilapia)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019347732.1	XP_019203277.1	protein NLRC3 isoform X1
XM_013267341.2	XP_013122795.2	protein NLRC3 isoform X2
XM_019347732.2	XP_019203277.1	NLR family CARD domain-containing protein 3 isoform X1
XM_019347731.1	XP_019203276.1	protein NLRC3 isoform X1
XM_019347733.2	XP_019203278.1	NLR family CARD domain-containing protein 3 isoform X1
XM_013267341.3	XP_013122795.2	NLR family CARD domain-containing protein 3 isoform X2
XM_019347733.1	XP_019203278.1	protein NLRC3 isoform X1
XM_019347731.2	XP_019203276.1	NLR family CARD domain-containing protein 3 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC102076273 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC102076273 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOr46341	XM_013267341.3 Latest version!	Oreochromis niloticus NLR family CARD domain-containing protein 3 (LOC102076273), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OOr46340	XM_019347733.2 Latest version!	Oreochromis niloticus NLR family CARD domain-containing protein 3 (LOC102076273), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OOr46340	XM_019347731.2 Latest version!	Oreochromis niloticus NLR family CARD domain-containing protein 3 (LOC102076273), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OOr46340	XM_019347732.2 Latest version!	Oreochromis niloticus NLR family CARD domain-containing protein 3 (LOC102076273), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OOr46340	XM_019347732.1	Oreochromis niloticus protein NLRC3 (LOC102076273), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OOr46341	XM_013267341.2	Oreochromis niloticus protein NLRC3 (LOC102076273), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OOr46340	XM_019347733.1	Oreochromis niloticus protein NLRC3 (LOC102076273), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OOr46340	XM_019347731.1	Oreochromis niloticus protein NLRC3 (LOC102076273), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC102076273 ( XM_013267341.2 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_013122795.2 Oreochromis niloticus protein NLRC3 (LOC102076273), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOr46341
Clone ID Related Accession (Same CDS sequence)	XM_013267341.2 , XM_013267341.3
Accession Version	XM_013267341.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	protein NLRC3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031982.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2016 this sequence version replaced XM_013267341.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATGC TGGAGGAAAA CATTATCACG TTTGTGAAGA ACGAGCTGAA GAAGATGCAG 
AATGATCTAA ATCCAGATTA TTCAGAGTAT CAGAGGGATG AAGAGGAGGT GTTGGACAGT 
GAGGAAGAGA AGCAGAGAAG GAGCAGCAGA GAGGCATTTC TGAAACTTGC AGTTAACTTC 
CTGAGAATAA TGAAACAGGA GCAGCTGGCT GACTGTCTGC AGAGCAAAAA TCCTGATCCA 
CTTTGTGAGA ACACATTTAA ATGTAACCTA AAGAAGAAGT TCCAGTGTGT CTCTGAGGGG 
ATCGCTAAAC CAGGAACCCG AACCACTCTA AATGAGATCT ACACAGAGCT CTGCATCACA 
GAGGGAGGGA CCGGAGAGGT CAACACTGAA CATGAGGTCA GACAGATTGA AATGTTTTCC 
TGGAAACCAA ACAGACCAGA AACATCAATC AGGTGTGAAG ACATCTTTAA AGCTTCGACT 
GAAGGAGACA AACCAATCAG AACAGTTCTG ACCAAAGGAG TGGCTGGCAT CGGGAAAACA 
CTCCTGACAC AAAAGTTCAC TTTGGACTGG GCTGAAGACA AGACCAACCA GGACATCCAG 
TTCATTTTTC CATTCACATT CAGAGAGCTG AATTTACTGA AGGATAAAAA GTTCAGCTTA 
GTGGGATTTA TTCATCACTT CTTCACTGAA ATCAAAGAAG CAGGAATCTG TAACTTTAAA 
AAGTTCAAAG TTGTGTTCAT CTTTGATGGT CTGGATGAGT GTCGACTTCC TCTGAACTTC 
CACATCACTG AGATCCTCAC TGATGTTACA GAGTCCACAT CAGTGGATGT GCTGCTGACA 
AACCTCATCA GGGGAAAGTT GCTTCCTTCT GCTCACATCT GGATAACCAC ACGACCTGCA 
GCAGCCAATC AGATCCCTGC TGACTATATA GACATGGTTA CAGAGGTCAG AGGGTTCACT 
GACCCAAAGA AGGAAGAGTA CTTTAAGAAG AAACTCAGAG ACAAGGAAGA AGCCAACACC 
ATAATCTCTC ACATCAAGAC ATCACGAAGC CTCCACATCA TGTGTCACAT CCCAATCTTC 
TGCTGGATCA CTGCTACAGT TTTAGAGAGT TTGTTAAAAA CCAAACAGGA TGGAGGAAAA 
CTGCCCAAGA CACTGACTGA GATGTACATT CACTTCCTGG TGGTTCAGAC CAAACTGAAG 
AACATCAAAT ATGATGGAGG AGCTGAGACA GATCCTCTCT GGAGTCCAGA GAACAGAAAG 
ATGATTGAAG CACTGGGGAA ACTGGCTTTT GAGCAGCTGC TGAAAGGAAA CCTGATCTTC 
TATGAAACAG ACCTGACAGA GTGTGGCATT AATGTCAGAG AGGCCTCAGT GTACTCAGGA 
GTGTTCACAC AGATTTTTAA AGAGGAGAGA GGACTGTACC AAGAGAAGGT GTTCTGTTTC 
ATCCATCTGA GCATTCAGGA GTTTTTAGCT GCTCTTTATG TCCATCTAAC ATTCACCAAC 
TCTGGTATCA ACCTGCTGAA AGAAGAAGAA ACAGCCTCAG GACAGACTGA CGAGTCTTCA 
GTAAGACAGT TCTACCAGAG TGCTGTGAAC GAGGCTTTGA AGAGTTCAAA TGGACACTTG 
GACTTGTTTC TTCGCTTCCT CCTTGGTCTT TCGATGCCAT CCAATCAGGA TCTCCTACAA 
GGCCTGCACA CACAAACAGG AAACAGGTCA CAGATAAATC AGGAAGCAGT CAAGTACATT 
AAAGAAACAA TGAATGACAC TCTCTCTTCA GAGAAAAGCA TCAATCTGCT CCACTGTCTG 
AATGAATTGA ATGATGACTC TATAGTGAAG GAGGTCCAGC ATCACCTGAG TTCAGGACAC 
CTGTCTAAAG TTAATCTCTC TCCTGCTCAG TGGTCAGCTC TGGTCTTCAT CTTACTGTCA 
TCAGAAACAG GTGTGGATGT GTTTGACTTG AGGAAATACT CAGCTTCAGA AGAGGCTCTT 
CTGCAGCTGC TGCCAGTGGT CAAAGTCTGT AAGAAAGCCC AGTTGAGTGG CTGTAACCTC 
ACAGACAGAA GCTGTGAAGC TCTTTCCTCA ATTCTAAGTT CTCAGTCCTC CAGACTGAGA 
GAGCTGGACA TGAGTAACAA CAACCTCCAG GACTCAGGAG TGAAGCTGCT TTGTGTTGGA 
CTTGGGAGTC CACACTGTAT GCTGGAAACT CTCAGGCTGT CAGGCTGTCA GATCACAGGA 
ACAGGCTTCA CCTCTCTGGC CACAGCTCTG TGCTCCAACC CCTCCTATTT GAAAGAGCTG 
GACTTGAGCT ATAATCACCC AGGAGACTCA GGAATGAAGC TTTTGTCTGC TCTAAAGGAG 
GATCCACATG TGAAACTCGA CATACTCTGT GTGGAACCTC AAGGAGTCCA GTGGATGAGA 
CCAGGTCTAA AGAAATATTT CTGTGATCTG ACGCTGGATC CAAACACAGC ACACAGAAAC 
CTCAAACTGT CTAAAAACAA CAAAGAGGTG ACACATGTGA AAGAAGACCA GCTGTATCCT 
GATCATGATC ATAGGTTTGA TCAGTGGCCT CAGCTGCTGT GTACCAACAG TCTGAGTGGT 
CGCTGTTACT GGGAGGTTGA GTGGAGTGGA GAGGTTCATG TTGCTGTAGT TTATGGAGGA 
ATCAGACGGA AAGGAGAAGA GGATGAAAGC AGGTTTGGAA GGACTGATCA ATCCTGGAGT 
CTCAACTGCA GTGATAATAA GGGTTACTAT GCAGGTCACA AATTTGATTT AAGAGCCATC 
CCTCTGCTCT CCTCAGCTCT TTCTCACAAA GTAGCAGTGT ATGTGGACTG TTCTGCAGGA 
ACTGTGTCTT TTTACAGTGT CTCCTGTGAT AAACTGATCC ACCTGCACAC CTTCAACACC 
ACCTTCACTG AACCTGTATA TGCTGGGTTT AGAGTGAAGC CTGACTCTAG TATCCACCTC 
TGTGCTGACT CAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013122795.2
CDS	13..2970
Translation	MNMLEENIITFVKNELKKMQNDLNPDYSEYQRDEEEVLDSEEEKQRRSSREAFLKLAVNFLRIMKQEQLADCLQSKNPDPLCENTFKCNLKKKFQCVSEGIAKPGTRTTLNEIYTELCITEGGTGEVNTEHEVRQIEMFSWKPNRPETSIRCEDIFKASTEGDKPIRTVLTKGVAGIGKTLLTQKFTLDWAEDKTNQDIQFIFPFTFRELNLLKDKKFSLVGFIHHFFTEIKEAGICNFKKFKVVFIFDGLDECRLPLNFHITEILTDVTESTSVDVLLTNLIRGKLLPSAHIWITTRPAAANQIPADYIDMVTEVRGFTDPKKEEYFKKKLRDKEEANTIISHIKTSRSLHIMCHIPIFCWITATVLESLLKTKQDGGKLPKTLTEMYIHFLVVQTKLKNIKYDGGAETDPLWSPENRKMIEALGKLAFEQLLKGNLIFYETDLTECGINVREASVYSGVFTQIFKEERGLYQEKVFCFIHLSIQEFLAALYVHLTFTNSGINLLKEEETASGQTDESSVRQFYQSAVNEALKSSNGHLDLFLRFLLGLSMPSNQDLLQGLHTQTGNRSQINQEAVKYIKETMNDTLSSEKSINLLHCLNELNDDSIVKEVQHHLSSGHLSKVNLSPAQWSALVFILLSSETGVDVFDLRKYSASEEALLQLLPVVKVCKKAQLSGCNLTDRSCEALSSILSSQSSRLRELDMSNNNLQDSGVKLLCVGLGSPHCMLETLRLSGCQITGTGFTSLATALCSNPSYLKELDLSYNHPGDSGMKLLSALKEDPHVKLDILCVEPQGVQWMRPGLKKYFCDLTLDPNTAHRNLKLSKNNKEVTHVKEDQLYPDHDHRFDQWPQLLCTNSLSGRCYWEVEWSGEVHVAVVYGGIRRKGEEDESRFGRTDQSWSLNCSDNKGYYAGHKFDLRAIPLLSSALSHKVAVYVDCSAGTVSFYSVSCDKLIHLHTFNTTFTEPVYAGFRVKPDSSIHLCADSE

Target ORF information:

RefSeq Version	XM_013267341.2
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus protein NLRC3 (LOC102076273), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013267341.2

ORF Insert Sequence:

ATGAACATGC TGGAGGAAAA CATTATCACG TTTGTGAAGA ACGAGCTGAA GAAGATGCAG 
AATGATCTAA ATCCAGATTA TTCAGAGTAT CAGAGGGATG AAGAGGAGGT GTTGGACAGT 
GAGGAAGAGA AGCAGAGAAG GAGCAGCAGA GAGGCATTTC TGAAACTTGC AGTTAACTTC 
CTGAGAATAA TGAAACAGGA GCAGCTGGCT GACTGTCTGC AGAGCAAAAA TCCTGATCCA 
CTTTGTGAGA ACACATTTAA ATGTAACCTA AAGAAGAAGT TCCAGTGTGT CTCTGAGGGG 
ATCGCTAAAC CAGGAACCCG AACCACTCTA AATGAGATCT ACACAGAGCT CTGCATCACA 
GAGGGAGGGA CCGGAGAGGT CAACACTGAA CATGAGGTCA GACAGATTGA AATGTTTTCC 
TGGAAACCAA ACAGACCAGA AACATCAATC AGGTGTGAAG ACATCTTTAA AGCTTCGACT 
GAAGGAGACA AACCAATCAG AACAGTTCTG ACCAAAGGAG TGGCTGGCAT CGGGAAAACA 
CTCCTGACAC AAAAGTTCAC TTTGGACTGG GCTGAAGACA AGACCAACCA GGACATCCAG 
TTCATTTTTC CATTCACATT CAGAGAGCTG AATTTACTGA AGGATAAAAA GTTCAGCTTA 
GTGGGATTTA TTCATCACTT CTTCACTGAA ATCAAAGAAG CAGGAATCTG TAACTTTAAA 
AAGTTCAAAG TTGTGTTCAT CTTTGATGGT CTGGATGAGT GTCGACTTCC TCTGAACTTC 
CACATCACTG AGATCCTCAC TGATGTTACA GAGTCCACAT CAGTGGATGT GCTGCTGACA 
AACCTCATCA GGGGAAAGTT GCTTCCTTCT GCTCACATCT GGATAACCAC ACGACCTGCA 
GCAGCCAATC AGATCCCTGC TGACTATATA GACATGGTTA CAGAGGTCAG AGGGTTCACT 
GACCCAAAGA AGGAAGAGTA CTTTAAGAAG AAACTCAGAG ACAAGGAAGA AGCCAACACC 
ATAATCTCTC ACATCAAGAC ATCACGAAGC CTCCACATCA TGTGTCACAT CCCAATCTTC 
TGCTGGATCA CTGCTACAGT TTTAGAGAGT TTGTTAAAAA CCAAACAGGA TGGAGGAAAA 
CTGCCCAAGA CACTGACTGA GATGTACATT CACTTCCTGG TGGTTCAGAC CAAACTGAAG 
AACATCAAAT ATGATGGAGG AGCTGAGACA GATCCTCTCT GGAGTCCAGA GAACAGAAAG 
ATGATTGAAG CACTGGGGAA ACTGGCTTTT GAGCAGCTGC TGAAAGGAAA CCTGATCTTC 
TATGAAACAG ACCTGACAGA GTGTGGCATT AATGTCAGAG AGGCCTCAGT GTACTCAGGA 
GTGTTCACAC AGATTTTTAA AGAGGAGAGA GGACTGTACC AAGAGAAGGT GTTCTGTTTC 
ATCCATCTGA GCATTCAGGA GTTTTTAGCT GCTCTTTATG TCCATCTAAC ATTCACCAAC 
TCTGGTATCA ACCTGCTGAA AGAAGAAGAA ACAGCCTCAG GACAGACTGA CGAGTCTTCA 
GTAAGACAGT TCTACCAGAG TGCTGTGAAC GAGGCTTTGA AGAGTTCAAA TGGACACTTG 
GACTTGTTTC TTCGCTTCCT CCTTGGTCTT TCGATGCCAT CCAATCAGGA TCTCCTACAA 
GGCCTGCACA CACAAACAGG AAACAGGTCA CAGATAAATC AGGAAGCAGT CAAGTACATT 
AAAGAAACAA TGAATGACAC TCTCTCTTCA GAGAAAAGCA TCAATCTGCT CCACTGTCTG 
AATGAATTGA ATGATGACTC TATAGTGAAG GAGGTCCAGC ATCACCTGAG TTCAGGACAC 
CTGTCTAAAG TTAATCTCTC TCCTGCTCAG TGGTCAGCTC TGGTCTTCAT CTTACTGTCA 
TCAGAAACAG GTGTGGATGT GTTTGACTTG AGGAAATACT CAGCTTCAGA AGAGGCTCTT 
CTGCAGCTGC TGCCAGTGGT CAAAGTCTGT AAGAAAGCCC AGTTGAGTGG CTGTAACCTC 
ACAGACAGAA GCTGTGAAGC TCTTTCCTCA ATTCTAAGTT CTCAGTCCTC CAGACTGAGA 
GAGCTGGACA TGAGTAACAA CAACCTCCAG GACTCAGGAG TGAAGCTGCT TTGTGTTGGA 
CTTGGGAGTC CACACTGTAT GCTGGAAACT CTCAGGCTGT CAGGCTGTCA GATCACAGGA 
ACAGGCTTCA CCTCTCTGGC CACAGCTCTG TGCTCCAACC CCTCCTATTT GAAAGAGCTG 
GACTTGAGCT ATAATCACCC AGGAGACTCA GGAATGAAGC TTTTGTCTGC TCTAAAGGAG 
GATCCACATG TGAAACTCGA CATACTCTGT GTGGAACCTC AAGGAGTCCA GTGGATGAGA 
CCAGGTCTAA AGAAATATTT CTGTGATCTG ACGCTGGATC CAAACACAGC ACACAGAAAC 
CTCAAACTGT CTAAAAACAA CAAAGAGGTG ACACATGTGA AAGAAGACCA GCTGTATCCT 
GATCATGATC ATAGGTTTGA TCAGTGGCCT CAGCTGCTGT GTACCAACAG TCTGAGTGGT 
CGCTGTTACT GGGAGGTTGA GTGGAGTGGA GAGGTTCATG TTGCTGTAGT TTATGGAGGA 
ATCAGACGGA AAGGAGAAGA GGATGAAAGC AGGTTTGGAA GGACTGATCA ATCCTGGAGT 
CTCAACTGCA GTGATAATAA GGGTTACTAT GCAGGTCACA AATTTGATTT AAGAGCCATC 
CCTCTGCTCT CCTCAGCTCT TTCTCACAAA GTAGCAGTGT ATGTGGACTG TTCTGCAGGA 
ACTGTGTCTT TTTACAGTGT CTCCTGTGAT AAACTGATCC ACCTGCACAC CTTCAACACC 
ACCTTCACTG AACCTGTATA TGCTGGGTTT AGAGTGAAGC CTGACTCTAG TATCCACCTC 
TGTGCTGACT CAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102076273 ( XM_013267341.3 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_013122795.2 Oreochromis niloticus NLR family CARD domain-containing protein 3 (LOC102076273), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOr46341
Clone ID Related Accession (Same CDS sequence)	XM_013267341.2 , XM_013267341.3
Accession Version	XM_013267341.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	NLR family CARD domain-containing protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031982.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_013267341.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATGC TGGAGGAAAA CATTATCACG TTTGTGAAGA ACGAGCTGAA GAAGATGCAG 
AATGATCTAA ATCCAGATTA TTCAGAGTAT CAGAGGGATG AAGAGGAGGT GTTGGACAGT 
GAGGAAGAGA AGCAGAGAAG GAGCAGCAGA GAGGCATTTC TGAAACTTGC AGTTAACTTC 
CTGAGAATAA TGAAACAGGA GCAGCTGGCT GACTGTCTGC AGAGCAAAAA TCCTGATCCA 
CTTTGTGAGA ACACATTTAA ATGTAACCTA AAGAAGAAGT TCCAGTGTGT CTCTGAGGGG 
ATCGCTAAAC CAGGAACCCG AACCACTCTA AATGAGATCT ACACAGAGCT CTGCATCACA 
GAGGGAGGGA CCGGAGAGGT CAACACTGAA CATGAGGTCA GACAGATTGA AATGTTTTCC 
TGGAAACCAA ACAGACCAGA AACATCAATC AGGTGTGAAG ACATCTTTAA AGCTTCGACT 
GAAGGAGACA AACCAATCAG AACAGTTCTG ACCAAAGGAG TGGCTGGCAT CGGGAAAACA 
CTCCTGACAC AAAAGTTCAC TTTGGACTGG GCTGAAGACA AGACCAACCA GGACATCCAG 
TTCATTTTTC CATTCACATT CAGAGAGCTG AATTTACTGA AGGATAAAAA GTTCAGCTTA 
GTGGGATTTA TTCATCACTT CTTCACTGAA ATCAAAGAAG CAGGAATCTG TAACTTTAAA 
AAGTTCAAAG TTGTGTTCAT CTTTGATGGT CTGGATGAGT GTCGACTTCC TCTGAACTTC 
CACATCACTG AGATCCTCAC TGATGTTACA GAGTCCACAT CAGTGGATGT GCTGCTGACA 
AACCTCATCA GGGGAAAGTT GCTTCCTTCT GCTCACATCT GGATAACCAC ACGACCTGCA 
GCAGCCAATC AGATCCCTGC TGACTATATA GACATGGTTA CAGAGGTCAG AGGGTTCACT 
GACCCAAAGA AGGAAGAGTA CTTTAAGAAG AAACTCAGAG ACAAGGAAGA AGCCAACACC 
ATAATCTCTC ACATCAAGAC ATCACGAAGC CTCCACATCA TGTGTCACAT CCCAATCTTC 
TGCTGGATCA CTGCTACAGT TTTAGAGAGT TTGTTAAAAA CCAAACAGGA TGGAGGAAAA 
CTGCCCAAGA CACTGACTGA GATGTACATT CACTTCCTGG TGGTTCAGAC CAAACTGAAG 
AACATCAAAT ATGATGGAGG AGCTGAGACA GATCCTCTCT GGAGTCCAGA GAACAGAAAG 
ATGATTGAAG CACTGGGGAA ACTGGCTTTT GAGCAGCTGC TGAAAGGAAA CCTGATCTTC 
TATGAAACAG ACCTGACAGA GTGTGGCATT AATGTCAGAG AGGCCTCAGT GTACTCAGGA 
GTGTTCACAC AGATTTTTAA AGAGGAGAGA GGACTGTACC AAGAGAAGGT GTTCTGTTTC 
ATCCATCTGA GCATTCAGGA GTTTTTAGCT GCTCTTTATG TCCATCTAAC ATTCACCAAC 
TCTGGTATCA ACCTGCTGAA AGAAGAAGAA ACAGCCTCAG GACAGACTGA CGAGTCTTCA 
GTAAGACAGT TCTACCAGAG TGCTGTGAAC GAGGCTTTGA AGAGTTCAAA TGGACACTTG 
GACTTGTTTC TTCGCTTCCT CCTTGGTCTT TCGATGCCAT CCAATCAGGA TCTCCTACAA 
GGCCTGCACA CACAAACAGG AAACAGGTCA CAGATAAATC AGGAAGCAGT CAAGTACATT 
AAAGAAACAA TGAATGACAC TCTCTCTTCA GAGAAAAGCA TCAATCTGCT CCACTGTCTG 
AATGAATTGA ATGATGACTC TATAGTGAAG GAGGTCCAGC ATCACCTGAG TTCAGGACAC 
CTGTCTAAAG TTAATCTCTC TCCTGCTCAG TGGTCAGCTC TGGTCTTCAT CTTACTGTCA 
TCAGAAACAG GTGTGGATGT GTTTGACTTG AGGAAATACT CAGCTTCAGA AGAGGCTCTT 
CTGCAGCTGC TGCCAGTGGT CAAAGTCTGT AAGAAAGCCC AGTTGAGTGG CTGTAACCTC 
ACAGACAGAA GCTGTGAAGC TCTTTCCTCA ATTCTAAGTT CTCAGTCCTC CAGACTGAGA 
GAGCTGGACA TGAGTAACAA CAACCTCCAG GACTCAGGAG TGAAGCTGCT TTGTGTTGGA 
CTTGGGAGTC CACACTGTAT GCTGGAAACT CTCAGGCTGT CAGGCTGTCA GATCACAGGA 
ACAGGCTTCA CCTCTCTGGC CACAGCTCTG TGCTCCAACC CCTCCTATTT GAAAGAGCTG 
GACTTGAGCT ATAATCACCC AGGAGACTCA GGAATGAAGC TTTTGTCTGC TCTAAAGGAG 
GATCCACATG TGAAACTCGA CATACTCTGT GTGGAACCTC AAGGAGTCCA GTGGATGAGA 
CCAGGTCTAA AGAAATATTT CTGTGATCTG ACGCTGGATC CAAACACAGC ACACAGAAAC 
CTCAAACTGT CTAAAAACAA CAAAGAGGTG ACACATGTGA AAGAAGACCA GCTGTATCCT 
GATCATGATC ATAGGTTTGA TCAGTGGCCT CAGCTGCTGT GTACCAACAG TCTGAGTGGT 
CGCTGTTACT GGGAGGTTGA GTGGAGTGGA GAGGTTCATG TTGCTGTAGT TTATGGAGGA 
ATCAGACGGA AAGGAGAAGA GGATGAAAGC AGGTTTGGAA GGACTGATCA ATCCTGGAGT 
CTCAACTGCA GTGATAATAA GGGTTACTAT GCAGGTCACA AATTTGATTT AAGAGCCATC 
CCTCTGCTCT CCTCAGCTCT TTCTCACAAA GTAGCAGTGT ATGTGGACTG TTCTGCAGGA 
ACTGTGTCTT TTTACAGTGT CTCCTGTGAT AAACTGATCC ACCTGCACAC CTTCAACACC 
ACCTTCACTG AACCTGTATA TGCTGGGTTT AGAGTGAAGC CTGACTCTAG TATCCACCTC 
TGTGCTGACT CAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013122795.2
CDS	13..2970
Translation	MNMLEENIITFVKNELKKMQNDLNPDYSEYQRDEEEVLDSEEEKQRRSSREAFLKLAVNFLRIMKQEQLADCLQSKNPDPLCENTFKCNLKKKFQCVSEGIAKPGTRTTLNEIYTELCITEGGTGEVNTEHEVRQIEMFSWKPNRPETSIRCEDIFKASTEGDKPIRTVLTKGVAGIGKTLLTQKFTLDWAEDKTNQDIQFIFPFTFRELNLLKDKKFSLVGFIHHFFTEIKEAGICNFKKFKVVFIFDGLDECRLPLNFHITEILTDVTESTSVDVLLTNLIRGKLLPSAHIWITTRPAAANQIPADYIDMVTEVRGFTDPKKEEYFKKKLRDKEEANTIISHIKTSRSLHIMCHIPIFCWITATVLESLLKTKQDGGKLPKTLTEMYIHFLVVQTKLKNIKYDGGAETDPLWSPENRKMIEALGKLAFEQLLKGNLIFYETDLTECGINVREASVYSGVFTQIFKEERGLYQEKVFCFIHLSIQEFLAALYVHLTFTNSGINLLKEEETASGQTDESSVRQFYQSAVNEALKSSNGHLDLFLRFLLGLSMPSNQDLLQGLHTQTGNRSQINQEAVKYIKETMNDTLSSEKSINLLHCLNELNDDSIVKEVQHHLSSGHLSKVNLSPAQWSALVFILLSSETGVDVFDLRKYSASEEALLQLLPVVKVCKKAQLSGCNLTDRSCEALSSILSSQSSRLRELDMSNNNLQDSGVKLLCVGLGSPHCMLETLRLSGCQITGTGFTSLATALCSNPSYLKELDLSYNHPGDSGMKLLSALKEDPHVKLDILCVEPQGVQWMRPGLKKYFCDLTLDPNTAHRNLKLSKNNKEVTHVKEDQLYPDHDHRFDQWPQLLCTNSLSGRCYWEVEWSGEVHVAVVYGGIRRKGEEDESRFGRTDQSWSLNCSDNKGYYAGHKFDLRAIPLLSSALSHKVAVYVDCSAGTVSFYSVSCDKLIHLHTFNTTFTEPVYAGFRVKPDSSIHLCADSE

Target ORF information:

RefSeq Version	XM_013267341.3
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus NLR family CARD domain-containing protein 3 (LOC102076273), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013267341.3

ORF Insert Sequence:

ATGAACATGC TGGAGGAAAA CATTATCACG TTTGTGAAGA ACGAGCTGAA GAAGATGCAG 
AATGATCTAA ATCCAGATTA TTCAGAGTAT CAGAGGGATG AAGAGGAGGT GTTGGACAGT 
GAGGAAGAGA AGCAGAGAAG GAGCAGCAGA GAGGCATTTC TGAAACTTGC AGTTAACTTC 
CTGAGAATAA TGAAACAGGA GCAGCTGGCT GACTGTCTGC AGAGCAAAAA TCCTGATCCA 
CTTTGTGAGA ACACATTTAA ATGTAACCTA AAGAAGAAGT TCCAGTGTGT CTCTGAGGGG 
ATCGCTAAAC CAGGAACCCG AACCACTCTA AATGAGATCT ACACAGAGCT CTGCATCACA 
GAGGGAGGGA CCGGAGAGGT CAACACTGAA CATGAGGTCA GACAGATTGA AATGTTTTCC 
TGGAAACCAA ACAGACCAGA AACATCAATC AGGTGTGAAG ACATCTTTAA AGCTTCGACT 
GAAGGAGACA AACCAATCAG AACAGTTCTG ACCAAAGGAG TGGCTGGCAT CGGGAAAACA 
CTCCTGACAC AAAAGTTCAC TTTGGACTGG GCTGAAGACA AGACCAACCA GGACATCCAG 
TTCATTTTTC CATTCACATT CAGAGAGCTG AATTTACTGA AGGATAAAAA GTTCAGCTTA 
GTGGGATTTA TTCATCACTT CTTCACTGAA ATCAAAGAAG CAGGAATCTG TAACTTTAAA 
AAGTTCAAAG TTGTGTTCAT CTTTGATGGT CTGGATGAGT GTCGACTTCC TCTGAACTTC 
CACATCACTG AGATCCTCAC TGATGTTACA GAGTCCACAT CAGTGGATGT GCTGCTGACA 
AACCTCATCA GGGGAAAGTT GCTTCCTTCT GCTCACATCT GGATAACCAC ACGACCTGCA 
GCAGCCAATC AGATCCCTGC TGACTATATA GACATGGTTA CAGAGGTCAG AGGGTTCACT 
GACCCAAAGA AGGAAGAGTA CTTTAAGAAG AAACTCAGAG ACAAGGAAGA AGCCAACACC 
ATAATCTCTC ACATCAAGAC ATCACGAAGC CTCCACATCA TGTGTCACAT CCCAATCTTC 
TGCTGGATCA CTGCTACAGT TTTAGAGAGT TTGTTAAAAA CCAAACAGGA TGGAGGAAAA 
CTGCCCAAGA CACTGACTGA GATGTACATT CACTTCCTGG TGGTTCAGAC CAAACTGAAG 
AACATCAAAT ATGATGGAGG AGCTGAGACA GATCCTCTCT GGAGTCCAGA GAACAGAAAG 
ATGATTGAAG CACTGGGGAA ACTGGCTTTT GAGCAGCTGC TGAAAGGAAA CCTGATCTTC 
TATGAAACAG ACCTGACAGA GTGTGGCATT AATGTCAGAG AGGCCTCAGT GTACTCAGGA 
GTGTTCACAC AGATTTTTAA AGAGGAGAGA GGACTGTACC AAGAGAAGGT GTTCTGTTTC 
ATCCATCTGA GCATTCAGGA GTTTTTAGCT GCTCTTTATG TCCATCTAAC ATTCACCAAC 
TCTGGTATCA ACCTGCTGAA AGAAGAAGAA ACAGCCTCAG GACAGACTGA CGAGTCTTCA 
GTAAGACAGT TCTACCAGAG TGCTGTGAAC GAGGCTTTGA AGAGTTCAAA TGGACACTTG 
GACTTGTTTC TTCGCTTCCT CCTTGGTCTT TCGATGCCAT CCAATCAGGA TCTCCTACAA 
GGCCTGCACA CACAAACAGG AAACAGGTCA CAGATAAATC AGGAAGCAGT CAAGTACATT 
AAAGAAACAA TGAATGACAC TCTCTCTTCA GAGAAAAGCA TCAATCTGCT CCACTGTCTG 
AATGAATTGA ATGATGACTC TATAGTGAAG GAGGTCCAGC ATCACCTGAG TTCAGGACAC 
CTGTCTAAAG TTAATCTCTC TCCTGCTCAG TGGTCAGCTC TGGTCTTCAT CTTACTGTCA 
TCAGAAACAG GTGTGGATGT GTTTGACTTG AGGAAATACT CAGCTTCAGA AGAGGCTCTT 
CTGCAGCTGC TGCCAGTGGT CAAAGTCTGT AAGAAAGCCC AGTTGAGTGG CTGTAACCTC 
ACAGACAGAA GCTGTGAAGC TCTTTCCTCA ATTCTAAGTT CTCAGTCCTC CAGACTGAGA 
GAGCTGGACA TGAGTAACAA CAACCTCCAG GACTCAGGAG TGAAGCTGCT TTGTGTTGGA 
CTTGGGAGTC CACACTGTAT GCTGGAAACT CTCAGGCTGT CAGGCTGTCA GATCACAGGA 
ACAGGCTTCA CCTCTCTGGC CACAGCTCTG TGCTCCAACC CCTCCTATTT GAAAGAGCTG 
GACTTGAGCT ATAATCACCC AGGAGACTCA GGAATGAAGC TTTTGTCTGC TCTAAAGGAG 
GATCCACATG TGAAACTCGA CATACTCTGT GTGGAACCTC AAGGAGTCCA GTGGATGAGA 
CCAGGTCTAA AGAAATATTT CTGTGATCTG ACGCTGGATC CAAACACAGC ACACAGAAAC 
CTCAAACTGT CTAAAAACAA CAAAGAGGTG ACACATGTGA AAGAAGACCA GCTGTATCCT 
GATCATGATC ATAGGTTTGA TCAGTGGCCT CAGCTGCTGT GTACCAACAG TCTGAGTGGT 
CGCTGTTACT GGGAGGTTGA GTGGAGTGGA GAGGTTCATG TTGCTGTAGT TTATGGAGGA 
ATCAGACGGA AAGGAGAAGA GGATGAAAGC AGGTTTGGAA GGACTGATCA ATCCTGGAGT 
CTCAACTGCA GTGATAATAA GGGTTACTAT GCAGGTCACA AATTTGATTT AAGAGCCATC 
CCTCTGCTCT CCTCAGCTCT TTCTCACAAA GTAGCAGTGT ATGTGGACTG TTCTGCAGGA 
ACTGTGTCTT TTTACAGTGT CTCCTGTGAT AAACTGATCC ACCTGCACAC CTTCAACACC 
ACCTTCACTG AACCTGTATA TGCTGGGTTT AGAGTGAAGC CTGACTCTAG TATCCACCTC 
TGTGCTGACT CAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102076273 ( XM_019347732.1 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_019203277.1 Oreochromis niloticus protein NLRC3 (LOC102076273), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOr46340
Clone ID Related Accession (Same CDS sequence)	XM_019347732.1 , XM_019347732.2 , XM_019347731.1 , XM_019347733.2 , XM_019347733.1 , XM_019347731.2
Accession Version	XM_019347732.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	protein NLRC3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031982.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAC CAGACTCTCC TGAGCCTGAG ACCGGACGCA GATCTCCACA GAAAGACCGT 
CCTGACTTTA AAATCAGGAA ACTGACTCCT GATGAGAGGA TCACTGAGAC ACCAGATGCC 
CTTGAATCTG AACCTGGACC CAGCTGTGTG TCTCTGAAGA GTGATCAGTC AAAGGATGGC 
ATGATCCTCT TTAAAGGAAT CAAATCTGCT TCTGCTGAGA AAGCTCATCA GGAGAGTCTG 
CAGCATCAAA CTGATCTGGA CTCAGTATTT AAGATGCTGG AGGAAAACAT TATCACGTTT 
GTGAAGAACG AGCTGAAGAA GATGCAGAAT GATCTAAATC CAGATTATTC AGAGTATCAG 
AGGGATGAAG AGGAGGTGTT GGACAGTGAG GAAGAGAAGC AGAGAAGGAG CAGCAGAGAG 
GCATTTCTGA AACTTGCAGT TAACTTCCTG AGAATAATGA AACAGGAGCA GCTGGCTGAC 
TGTCTGCAGA GCAAAAATCC TGATCCACTT TGTGAGAACA CATTTAAATG TAACCTAAAG 
AAGAAGTTCC AGTGTGTCTC TGAGGGGATC GCTAAACCAG GAACCCGAAC CACTCTAAAT 
GAGATCTACA CAGAGCTCTG CATCACAGAG GGAGGGACCG GAGAGGTCAA CACTGAACAT 
GAGGTCAGAC AGATTGAAAT GTTTTCCTGG AAACCAAACA GACCAGAAAC ATCAATCAGG 
TGTGAAGACA TCTTTAAAGC TTCGACTGAA GGAGACAAAC CAATCAGAAC AGTTCTGACC 
AAAGGAGTGG CTGGCATCGG GAAAACACTC CTGACACAAA AGTTCACTTT GGACTGGGCT 
GAAGACAAGA CCAACCAGGA CATCCAGTTC ATTTTTCCAT TCACATTCAG AGAGCTGAAT 
TTACTGAAGG ATAAAAAGTT CAGCTTAGTG GGATTTATTC ATCACTTCTT CACTGAAATC 
AAAGAAGCAG GAATCTGTAA CTTTAAAAAG TTCAAAGTTG TGTTCATCTT TGATGGTCTG 
GATGAGTGTC GACTTCCTCT GAACTTCCAC ATCACTGAGA TCCTCACTGA TGTTACAGAG 
TCCACATCAG TGGATGTGCT GCTGACAAAC CTCATCAGGG GAAAGTTGCT TCCTTCTGCT 
CACATCTGGA TAACCACACG ACCTGCAGCA GCCAATCAGA TCCCTGCTGA CTATATAGAC 
ATGGTTACAG AGGTCAGAGG GTTCACTGAC CCAAAGAAGG AAGAGTACTT TAAGAAGAAA 
CTCAGAGACA AGGAAGAAGC CAACACCATA ATCTCTCACA TCAAGACATC ACGAAGCCTC 
CACATCATGT GTCACATCCC AATCTTCTGC TGGATCACTG CTACAGTTTT AGAGAGTTTG 
TTAAAAACCA AACAGGATGG AGGAAAACTG CCCAAGACAC TGACTGAGAT GTACATTCAC 
TTCCTGGTGG TTCAGACCAA ACTGAAGAAC ATCAAATATG ATGGAGGAGC TGAGACAGAT 
CCTCTCTGGA GTCCAGAGAA CAGAAAGATG ATTGAAGCAC TGGGGAAACT GGCTTTTGAG 
CAGCTGCTGA AAGGAAACCT GATCTTCTAT GAAACAGACC TGACAGAGTG TGGCATTAAT 
GTCAGAGAGG CCTCAGTGTA CTCAGGAGTG TTCACACAGA TTTTTAAAGA GGAGAGAGGA 
CTGTACCAAG AGAAGGTGTT CTGTTTCATC CATCTGAGCA TTCAGGAGTT TTTAGCTGCT 
CTTTATGTCC ATCTAACATT CACCAACTCT GGTATCAACC TGCTGAAAGA AGAAGAAACA 
GCCTCAGGAC AGACTGACGA GTCTTCAGTA AGACAGTTCT ACCAGAGTGC TGTGAACGAG 
GCTTTGAAGA GTTCAAATGG ACACTTGGAC TTGTTTCTTC GCTTCCTCCT TGGTCTTTCG 
ATGCCATCCA ATCAGGATCT CCTACAAGGC CTGCACACAC AAACAGGAAA CAGGTCACAG 
ATAAATCAGG AAGCAGTCAA GTACATTAAA GAAACAATGA ATGACACTCT CTCTTCAGAG 
AAAAGCATCA ATCTGCTCCA CTGTCTGAAT GAATTGAATG ATGACTCTAT AGTGAAGGAG 
GTCCAGCATC ACCTGAGTTC AGGACACCTG TCTAAAGTTA ATCTCTCTCC TGCTCAGTGG 
TCAGCTCTGG TCTTCATCTT ACTGTCATCA GAAACAGGTG TGGATGTGTT TGACTTGAGG 
AAATACTCAG CTTCAGAAGA GGCTCTTCTG CAGCTGCTGC CAGTGGTCAA AGTCTGTAAG 
AAAGCCCAGT TGAGTGGCTG TAACCTCACA GACAGAAGCT GTGAAGCTCT TTCCTCAATT 
CTAAGTTCTC AGTCCTCCAG ACTGAGAGAG CTGGACATGA GTAACAACAA CCTCCAGGAC 
TCAGGAGTGA AGCTGCTTTG TGTTGGACTT GGGAGTCCAC ACTGTATGCT GGAAACTCTC 
AGGCTGTCAG GCTGTCAGAT CACAGGAACA GGCTTCACCT CTCTGGCCAC AGCTCTGTGC 
TCCAACCCCT CCTATTTGAA AGAGCTGGAC TTGAGCTATA ATCACCCAGG AGACTCAGGA 
ATGAAGCTTT TGTCTGCTCT AAAGGAGGAT CCACATGTGA AACTCGACAT ACTCTGTGTG 
GAACCTCAAG GAGTCCAGTG GATGAGACCA GGTCTAAAGA AATATTTCTG TGATCTGACG 
CTGGATCCAA ACACAGCACA CAGAAACCTC AAACTGTCTA AAAACAACAA AGAGGTGACA 
CATGTGAAAG AAGACCAGCT GTATCCTGAT CATGATCATA GGTTTGATCA GTGGCCTCAG 
CTGCTGTGTA CCAACAGTCT GAGTGGTCGC TGTTACTGGG AGGTTGAGTG GAGTGGAGAG 
GTTCATGTTG CTGTAGTTTA TGGAGGAATC AGACGGAAAG GAGAAGAGGA TGAAAGCAGG 
TTTGGAAGGA CTGATCAATC CTGGAGTCTC AACTGCAGTG ATAATAAGGG TTACTATGCA 
GGTCACAAAT TTGATTTAAG AGCCATCCCT CTGCTCTCCT CAGCTCTTTC TCACAAAGTA 
GCAGTGTATG TGGACTGTTC TGCAGGAACT GTGTCTTTTT ACAGTGTCTC CTGTGATAAA 
CTGATCCACC TGCACACCTT CAACACCACC TTCACTGAAC CTGTATATGC TGGGTTTAGA 
GTGAAGCCTG ACTCTAGTAT CCACCTCTGT GCTGACTCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019203277.1
CDS	220..3444
Translation	MERPDSPEPETGRRSPQKDRPDFKIRKLTPDERITETPDALESEPGPSCVSLKSDQSKDGMILFKGIKSASAEKAHQESLQHQTDLDSVFKMLEENIITFVKNELKKMQNDLNPDYSEYQRDEEEVLDSEEEKQRRSSREAFLKLAVNFLRIMKQEQLADCLQSKNPDPLCENTFKCNLKKKFQCVSEGIAKPGTRTTLNEIYTELCITEGGTGEVNTEHEVRQIEMFSWKPNRPETSIRCEDIFKASTEGDKPIRTVLTKGVAGIGKTLLTQKFTLDWAEDKTNQDIQFIFPFTFRELNLLKDKKFSLVGFIHHFFTEIKEAGICNFKKFKVVFIFDGLDECRLPLNFHITEILTDVTESTSVDVLLTNLIRGKLLPSAHIWITTRPAAANQIPADYIDMVTEVRGFTDPKKEEYFKKKLRDKEEANTIISHIKTSRSLHIMCHIPIFCWITATVLESLLKTKQDGGKLPKTLTEMYIHFLVVQTKLKNIKYDGGAETDPLWSPENRKMIEALGKLAFEQLLKGNLIFYETDLTECGINVREASVYSGVFTQIFKEERGLYQEKVFCFIHLSIQEFLAALYVHLTFTNSGINLLKEEETASGQTDESSVRQFYQSAVNEALKSSNGHLDLFLRFLLGLSMPSNQDLLQGLHTQTGNRSQINQEAVKYIKETMNDTLSSEKSINLLHCLNELNDDSIVKEVQHHLSSGHLSKVNLSPAQWSALVFILLSSETGVDVFDLRKYSASEEALLQLLPVVKVCKKAQLSGCNLTDRSCEALSSILSSQSSRLRELDMSNNNLQDSGVKLLCVGLGSPHCMLETLRLSGCQITGTGFTSLATALCSNPSYLKELDLSYNHPGDSGMKLLSALKEDPHVKLDILCVEPQGVQWMRPGLKKYFCDLTLDPNTAHRNLKLSKNNKEVTHVKEDQLYPDHDHRFDQWPQLLCTNSLSGRCYWEVEWSGEVHVAVVYGGIRRKGEEDESRFGRTDQSWSLNCSDNKGYYAGHKFDLRAIPLLSSALSHKVAVYVDCSAGTVSFYSVSCDKLIHLHTFNTTFTEPVYAGFRVKPDSSIHLCADSE

Target ORF information:

RefSeq Version	XM_019347732.1
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus protein NLRC3 (LOC102076273), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019347732.1

ORF Insert Sequence:

ATGGAAAGAC CAGACTCTCC TGAGCCTGAG ACCGGACGCA GATCTCCACA GAAAGACCGT 
CCTGACTTTA AAATCAGGAA ACTGACTCCT GATGAGAGGA TCACTGAGAC ACCAGATGCC 
CTTGAATCTG AACCTGGACC CAGCTGTGTG TCTCTGAAGA GTGATCAGTC AAAGGATGGC 
ATGATCCTCT TTAAAGGAAT CAAATCTGCT TCTGCTGAGA AAGCTCATCA GGAGAGTCTG 
CAGCATCAAA CTGATCTGGA CTCAGTATTT AAGATGCTGG AGGAAAACAT TATCACGTTT 
GTGAAGAACG AGCTGAAGAA GATGCAGAAT GATCTAAATC CAGATTATTC AGAGTATCAG 
AGGGATGAAG AGGAGGTGTT GGACAGTGAG GAAGAGAAGC AGAGAAGGAG CAGCAGAGAG 
GCATTTCTGA AACTTGCAGT TAACTTCCTG AGAATAATGA AACAGGAGCA GCTGGCTGAC 
TGTCTGCAGA GCAAAAATCC TGATCCACTT TGTGAGAACA CATTTAAATG TAACCTAAAG 
AAGAAGTTCC AGTGTGTCTC TGAGGGGATC GCTAAACCAG GAACCCGAAC CACTCTAAAT 
GAGATCTACA CAGAGCTCTG CATCACAGAG GGAGGGACCG GAGAGGTCAA CACTGAACAT 
GAGGTCAGAC AGATTGAAAT GTTTTCCTGG AAACCAAACA GACCAGAAAC ATCAATCAGG 
TGTGAAGACA TCTTTAAAGC TTCGACTGAA GGAGACAAAC CAATCAGAAC AGTTCTGACC 
AAAGGAGTGG CTGGCATCGG GAAAACACTC CTGACACAAA AGTTCACTTT GGACTGGGCT 
GAAGACAAGA CCAACCAGGA CATCCAGTTC ATTTTTCCAT TCACATTCAG AGAGCTGAAT 
TTACTGAAGG ATAAAAAGTT CAGCTTAGTG GGATTTATTC ATCACTTCTT CACTGAAATC 
AAAGAAGCAG GAATCTGTAA CTTTAAAAAG TTCAAAGTTG TGTTCATCTT TGATGGTCTG 
GATGAGTGTC GACTTCCTCT GAACTTCCAC ATCACTGAGA TCCTCACTGA TGTTACAGAG 
TCCACATCAG TGGATGTGCT GCTGACAAAC CTCATCAGGG GAAAGTTGCT TCCTTCTGCT 
CACATCTGGA TAACCACACG ACCTGCAGCA GCCAATCAGA TCCCTGCTGA CTATATAGAC 
ATGGTTACAG AGGTCAGAGG GTTCACTGAC CCAAAGAAGG AAGAGTACTT TAAGAAGAAA 
CTCAGAGACA AGGAAGAAGC CAACACCATA ATCTCTCACA TCAAGACATC ACGAAGCCTC 
CACATCATGT GTCACATCCC AATCTTCTGC TGGATCACTG CTACAGTTTT AGAGAGTTTG 
TTAAAAACCA AACAGGATGG AGGAAAACTG CCCAAGACAC TGACTGAGAT GTACATTCAC 
TTCCTGGTGG TTCAGACCAA ACTGAAGAAC ATCAAATATG ATGGAGGAGC TGAGACAGAT 
CCTCTCTGGA GTCCAGAGAA CAGAAAGATG ATTGAAGCAC TGGGGAAACT GGCTTTTGAG 
CAGCTGCTGA AAGGAAACCT GATCTTCTAT GAAACAGACC TGACAGAGTG TGGCATTAAT 
GTCAGAGAGG CCTCAGTGTA CTCAGGAGTG TTCACACAGA TTTTTAAAGA GGAGAGAGGA 
CTGTACCAAG AGAAGGTGTT CTGTTTCATC CATCTGAGCA TTCAGGAGTT TTTAGCTGCT 
CTTTATGTCC ATCTAACATT CACCAACTCT GGTATCAACC TGCTGAAAGA AGAAGAAACA 
GCCTCAGGAC AGACTGACGA GTCTTCAGTA AGACAGTTCT ACCAGAGTGC TGTGAACGAG 
GCTTTGAAGA GTTCAAATGG ACACTTGGAC TTGTTTCTTC GCTTCCTCCT TGGTCTTTCG 
ATGCCATCCA ATCAGGATCT CCTACAAGGC CTGCACACAC AAACAGGAAA CAGGTCACAG 
ATAAATCAGG AAGCAGTCAA GTACATTAAA GAAACAATGA ATGACACTCT CTCTTCAGAG 
AAAAGCATCA ATCTGCTCCA CTGTCTGAAT GAATTGAATG ATGACTCTAT AGTGAAGGAG 
GTCCAGCATC ACCTGAGTTC AGGACACCTG TCTAAAGTTA ATCTCTCTCC TGCTCAGTGG 
TCAGCTCTGG TCTTCATCTT ACTGTCATCA GAAACAGGTG TGGATGTGTT TGACTTGAGG 
AAATACTCAG CTTCAGAAGA GGCTCTTCTG CAGCTGCTGC CAGTGGTCAA AGTCTGTAAG 
AAAGCCCAGT TGAGTGGCTG TAACCTCACA GACAGAAGCT GTGAAGCTCT TTCCTCAATT 
CTAAGTTCTC AGTCCTCCAG ACTGAGAGAG CTGGACATGA GTAACAACAA CCTCCAGGAC 
TCAGGAGTGA AGCTGCTTTG TGTTGGACTT GGGAGTCCAC ACTGTATGCT GGAAACTCTC 
AGGCTGTCAG GCTGTCAGAT CACAGGAACA GGCTTCACCT CTCTGGCCAC AGCTCTGTGC 
TCCAACCCCT CCTATTTGAA AGAGCTGGAC TTGAGCTATA ATCACCCAGG AGACTCAGGA 
ATGAAGCTTT TGTCTGCTCT AAAGGAGGAT CCACATGTGA AACTCGACAT ACTCTGTGTG 
GAACCTCAAG GAGTCCAGTG GATGAGACCA GGTCTAAAGA AATATTTCTG TGATCTGACG 
CTGGATCCAA ACACAGCACA CAGAAACCTC AAACTGTCTA AAAACAACAA AGAGGTGACA 
CATGTGAAAG AAGACCAGCT GTATCCTGAT CATGATCATA GGTTTGATCA GTGGCCTCAG 
CTGCTGTGTA CCAACAGTCT GAGTGGTCGC TGTTACTGGG AGGTTGAGTG GAGTGGAGAG 
GTTCATGTTG CTGTAGTTTA TGGAGGAATC AGACGGAAAG GAGAAGAGGA TGAAAGCAGG 
TTTGGAAGGA CTGATCAATC CTGGAGTCTC AACTGCAGTG ATAATAAGGG TTACTATGCA 
GGTCACAAAT TTGATTTAAG AGCCATCCCT CTGCTCTCCT CAGCTCTTTC TCACAAAGTA 
GCAGTGTATG TGGACTGTTC TGCAGGAACT GTGTCTTTTT ACAGTGTCTC CTGTGATAAA 
CTGATCCACC TGCACACCTT CAACACCACC TTCACTGAAC CTGTATATGC TGGGTTTAGA 
GTGAAGCCTG ACTCTAGTAT CCACCTCTGT GCTGACTCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102076273 ( XM_019347732.2 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_019203277.1 Oreochromis niloticus NLR family CARD domain-containing protein 3 (LOC102076273), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOr46340
Clone ID Related Accession (Same CDS sequence)	XM_019347732.1 , XM_019347732.2 , XM_019347731.1 , XM_019347733.2 , XM_019347733.1 , XM_019347731.2
Accession Version	XM_019347732.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	NLR family CARD domain-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031982.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_019347732.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAC CAGACTCTCC TGAGCCTGAG ACCGGACGCA GATCTCCACA GAAAGACCGT 
CCTGACTTTA AAATCAGGAA ACTGACTCCT GATGAGAGGA TCACTGAGAC ACCAGATGCC 
CTTGAATCTG AACCTGGACC CAGCTGTGTG TCTCTGAAGA GTGATCAGTC AAAGGATGGC 
ATGATCCTCT TTAAAGGAAT CAAATCTGCT TCTGCTGAGA AAGCTCATCA GGAGAGTCTG 
CAGCATCAAA CTGATCTGGA CTCAGTATTT AAGATGCTGG AGGAAAACAT TATCACGTTT 
GTGAAGAACG AGCTGAAGAA GATGCAGAAT GATCTAAATC CAGATTATTC AGAGTATCAG 
AGGGATGAAG AGGAGGTGTT GGACAGTGAG GAAGAGAAGC AGAGAAGGAG CAGCAGAGAG 
GCATTTCTGA AACTTGCAGT TAACTTCCTG AGAATAATGA AACAGGAGCA GCTGGCTGAC 
TGTCTGCAGA GCAAAAATCC TGATCCACTT TGTGAGAACA CATTTAAATG TAACCTAAAG 
AAGAAGTTCC AGTGTGTCTC TGAGGGGATC GCTAAACCAG GAACCCGAAC CACTCTAAAT 
GAGATCTACA CAGAGCTCTG CATCACAGAG GGAGGGACCG GAGAGGTCAA CACTGAACAT 
GAGGTCAGAC AGATTGAAAT GTTTTCCTGG AAACCAAACA GACCAGAAAC ATCAATCAGG 
TGTGAAGACA TCTTTAAAGC TTCGACTGAA GGAGACAAAC CAATCAGAAC AGTTCTGACC 
AAAGGAGTGG CTGGCATCGG GAAAACACTC CTGACACAAA AGTTCACTTT GGACTGGGCT 
GAAGACAAGA CCAACCAGGA CATCCAGTTC ATTTTTCCAT TCACATTCAG AGAGCTGAAT 
TTACTGAAGG ATAAAAAGTT CAGCTTAGTG GGATTTATTC ATCACTTCTT CACTGAAATC 
AAAGAAGCAG GAATCTGTAA CTTTAAAAAG TTCAAAGTTG TGTTCATCTT TGATGGTCTG 
GATGAGTGTC GACTTCCTCT GAACTTCCAC ATCACTGAGA TCCTCACTGA TGTTACAGAG 
TCCACATCAG TGGATGTGCT GCTGACAAAC CTCATCAGGG GAAAGTTGCT TCCTTCTGCT 
CACATCTGGA TAACCACACG ACCTGCAGCA GCCAATCAGA TCCCTGCTGA CTATATAGAC 
ATGGTTACAG AGGTCAGAGG GTTCACTGAC CCAAAGAAGG AAGAGTACTT TAAGAAGAAA 
CTCAGAGACA AGGAAGAAGC CAACACCATA ATCTCTCACA TCAAGACATC ACGAAGCCTC 
CACATCATGT GTCACATCCC AATCTTCTGC TGGATCACTG CTACAGTTTT AGAGAGTTTG 
TTAAAAACCA AACAGGATGG AGGAAAACTG CCCAAGACAC TGACTGAGAT GTACATTCAC 
TTCCTGGTGG TTCAGACCAA ACTGAAGAAC ATCAAATATG ATGGAGGAGC TGAGACAGAT 
CCTCTCTGGA GTCCAGAGAA CAGAAAGATG ATTGAAGCAC TGGGGAAACT GGCTTTTGAG 
CAGCTGCTGA AAGGAAACCT GATCTTCTAT GAAACAGACC TGACAGAGTG TGGCATTAAT 
GTCAGAGAGG CCTCAGTGTA CTCAGGAGTG TTCACACAGA TTTTTAAAGA GGAGAGAGGA 
CTGTACCAAG AGAAGGTGTT CTGTTTCATC CATCTGAGCA TTCAGGAGTT TTTAGCTGCT 
CTTTATGTCC ATCTAACATT CACCAACTCT GGTATCAACC TGCTGAAAGA AGAAGAAACA 
GCCTCAGGAC AGACTGACGA GTCTTCAGTA AGACAGTTCT ACCAGAGTGC TGTGAACGAG 
GCTTTGAAGA GTTCAAATGG ACACTTGGAC TTGTTTCTTC GCTTCCTCCT TGGTCTTTCG 
ATGCCATCCA ATCAGGATCT CCTACAAGGC CTGCACACAC AAACAGGAAA CAGGTCACAG 
ATAAATCAGG AAGCAGTCAA GTACATTAAA GAAACAATGA ATGACACTCT CTCTTCAGAG 
AAAAGCATCA ATCTGCTCCA CTGTCTGAAT GAATTGAATG ATGACTCTAT AGTGAAGGAG 
GTCCAGCATC ACCTGAGTTC AGGACACCTG TCTAAAGTTA ATCTCTCTCC TGCTCAGTGG 
TCAGCTCTGG TCTTCATCTT ACTGTCATCA GAAACAGGTG TGGATGTGTT TGACTTGAGG 
AAATACTCAG CTTCAGAAGA GGCTCTTCTG CAGCTGCTGC CAGTGGTCAA AGTCTGTAAG 
AAAGCCCAGT TGAGTGGCTG TAACCTCACA GACAGAAGCT GTGAAGCTCT TTCCTCAATT 
CTAAGTTCTC AGTCCTCCAG ACTGAGAGAG CTGGACATGA GTAACAACAA CCTCCAGGAC 
TCAGGAGTGA AGCTGCTTTG TGTTGGACTT GGGAGTCCAC ACTGTATGCT GGAAACTCTC 
AGGCTGTCAG GCTGTCAGAT CACAGGAACA GGCTTCACCT CTCTGGCCAC AGCTCTGTGC 
TCCAACCCCT CCTATTTGAA AGAGCTGGAC TTGAGCTATA ATCACCCAGG AGACTCAGGA 
ATGAAGCTTT TGTCTGCTCT AAAGGAGGAT CCACATGTGA AACTCGACAT ACTCTGTGTG 
GAACCTCAAG GAGTCCAGTG GATGAGACCA GGTCTAAAGA AATATTTCTG TGATCTGACG 
CTGGATCCAA ACACAGCACA CAGAAACCTC AAACTGTCTA AAAACAACAA AGAGGTGACA 
CATGTGAAAG AAGACCAGCT GTATCCTGAT CATGATCATA GGTTTGATCA GTGGCCTCAG 
CTGCTGTGTA CCAACAGTCT GAGTGGTCGC TGTTACTGGG AGGTTGAGTG GAGTGGAGAG 
GTTCATGTTG CTGTAGTTTA TGGAGGAATC AGACGGAAAG GAGAAGAGGA TGAAAGCAGG 
TTTGGAAGGA CTGATCAATC CTGGAGTCTC AACTGCAGTG ATAATAAGGG TTACTATGCA 
GGTCACAAAT TTGATTTAAG AGCCATCCCT CTGCTCTCCT CAGCTCTTTC TCACAAAGTA 
GCAGTGTATG TGGACTGTTC TGCAGGAACT GTGTCTTTTT ACAGTGTCTC CTGTGATAAA 
CTGATCCACC TGCACACCTT CAACACCACC TTCACTGAAC CTGTATATGC TGGGTTTAGA 
GTGAAGCCTG ACTCTAGTAT CCACCTCTGT GCTGACTCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019203277.1
CDS	238..3462
Translation	MERPDSPEPETGRRSPQKDRPDFKIRKLTPDERITETPDALESEPGPSCVSLKSDQSKDGMILFKGIKSASAEKAHQESLQHQTDLDSVFKMLEENIITFVKNELKKMQNDLNPDYSEYQRDEEEVLDSEEEKQRRSSREAFLKLAVNFLRIMKQEQLADCLQSKNPDPLCENTFKCNLKKKFQCVSEGIAKPGTRTTLNEIYTELCITEGGTGEVNTEHEVRQIEMFSWKPNRPETSIRCEDIFKASTEGDKPIRTVLTKGVAGIGKTLLTQKFTLDWAEDKTNQDIQFIFPFTFRELNLLKDKKFSLVGFIHHFFTEIKEAGICNFKKFKVVFIFDGLDECRLPLNFHITEILTDVTESTSVDVLLTNLIRGKLLPSAHIWITTRPAAANQIPADYIDMVTEVRGFTDPKKEEYFKKKLRDKEEANTIISHIKTSRSLHIMCHIPIFCWITATVLESLLKTKQDGGKLPKTLTEMYIHFLVVQTKLKNIKYDGGAETDPLWSPENRKMIEALGKLAFEQLLKGNLIFYETDLTECGINVREASVYSGVFTQIFKEERGLYQEKVFCFIHLSIQEFLAALYVHLTFTNSGINLLKEEETASGQTDESSVRQFYQSAVNEALKSSNGHLDLFLRFLLGLSMPSNQDLLQGLHTQTGNRSQINQEAVKYIKETMNDTLSSEKSINLLHCLNELNDDSIVKEVQHHLSSGHLSKVNLSPAQWSALVFILLSSETGVDVFDLRKYSASEEALLQLLPVVKVCKKAQLSGCNLTDRSCEALSSILSSQSSRLRELDMSNNNLQDSGVKLLCVGLGSPHCMLETLRLSGCQITGTGFTSLATALCSNPSYLKELDLSYNHPGDSGMKLLSALKEDPHVKLDILCVEPQGVQWMRPGLKKYFCDLTLDPNTAHRNLKLSKNNKEVTHVKEDQLYPDHDHRFDQWPQLLCTNSLSGRCYWEVEWSGEVHVAVVYGGIRRKGEEDESRFGRTDQSWSLNCSDNKGYYAGHKFDLRAIPLLSSALSHKVAVYVDCSAGTVSFYSVSCDKLIHLHTFNTTFTEPVYAGFRVKPDSSIHLCADSE

Target ORF information:

RefSeq Version	XM_019347732.2
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus NLR family CARD domain-containing protein 3 (LOC102076273), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019347732.2

ORF Insert Sequence:

ATGGAAAGAC CAGACTCTCC TGAGCCTGAG ACCGGACGCA GATCTCCACA GAAAGACCGT 
CCTGACTTTA AAATCAGGAA ACTGACTCCT GATGAGAGGA TCACTGAGAC ACCAGATGCC 
CTTGAATCTG AACCTGGACC CAGCTGTGTG TCTCTGAAGA GTGATCAGTC AAAGGATGGC 
ATGATCCTCT TTAAAGGAAT CAAATCTGCT TCTGCTGAGA AAGCTCATCA GGAGAGTCTG 
CAGCATCAAA CTGATCTGGA CTCAGTATTT AAGATGCTGG AGGAAAACAT TATCACGTTT 
GTGAAGAACG AGCTGAAGAA GATGCAGAAT GATCTAAATC CAGATTATTC AGAGTATCAG 
AGGGATGAAG AGGAGGTGTT GGACAGTGAG GAAGAGAAGC AGAGAAGGAG CAGCAGAGAG 
GCATTTCTGA AACTTGCAGT TAACTTCCTG AGAATAATGA AACAGGAGCA GCTGGCTGAC 
TGTCTGCAGA GCAAAAATCC TGATCCACTT TGTGAGAACA CATTTAAATG TAACCTAAAG 
AAGAAGTTCC AGTGTGTCTC TGAGGGGATC GCTAAACCAG GAACCCGAAC CACTCTAAAT 
GAGATCTACA CAGAGCTCTG CATCACAGAG GGAGGGACCG GAGAGGTCAA CACTGAACAT 
GAGGTCAGAC AGATTGAAAT GTTTTCCTGG AAACCAAACA GACCAGAAAC ATCAATCAGG 
TGTGAAGACA TCTTTAAAGC TTCGACTGAA GGAGACAAAC CAATCAGAAC AGTTCTGACC 
AAAGGAGTGG CTGGCATCGG GAAAACACTC CTGACACAAA AGTTCACTTT GGACTGGGCT 
GAAGACAAGA CCAACCAGGA CATCCAGTTC ATTTTTCCAT TCACATTCAG AGAGCTGAAT 
TTACTGAAGG ATAAAAAGTT CAGCTTAGTG GGATTTATTC ATCACTTCTT CACTGAAATC 
AAAGAAGCAG GAATCTGTAA CTTTAAAAAG TTCAAAGTTG TGTTCATCTT TGATGGTCTG 
GATGAGTGTC GACTTCCTCT GAACTTCCAC ATCACTGAGA TCCTCACTGA TGTTACAGAG 
TCCACATCAG TGGATGTGCT GCTGACAAAC CTCATCAGGG GAAAGTTGCT TCCTTCTGCT 
CACATCTGGA TAACCACACG ACCTGCAGCA GCCAATCAGA TCCCTGCTGA CTATATAGAC 
ATGGTTACAG AGGTCAGAGG GTTCACTGAC CCAAAGAAGG AAGAGTACTT TAAGAAGAAA 
CTCAGAGACA AGGAAGAAGC CAACACCATA ATCTCTCACA TCAAGACATC ACGAAGCCTC 
CACATCATGT GTCACATCCC AATCTTCTGC TGGATCACTG CTACAGTTTT AGAGAGTTTG 
TTAAAAACCA AACAGGATGG AGGAAAACTG CCCAAGACAC TGACTGAGAT GTACATTCAC 
TTCCTGGTGG TTCAGACCAA ACTGAAGAAC ATCAAATATG ATGGAGGAGC TGAGACAGAT 
CCTCTCTGGA GTCCAGAGAA CAGAAAGATG ATTGAAGCAC TGGGGAAACT GGCTTTTGAG 
CAGCTGCTGA AAGGAAACCT GATCTTCTAT GAAACAGACC TGACAGAGTG TGGCATTAAT 
GTCAGAGAGG CCTCAGTGTA CTCAGGAGTG TTCACACAGA TTTTTAAAGA GGAGAGAGGA 
CTGTACCAAG AGAAGGTGTT CTGTTTCATC CATCTGAGCA TTCAGGAGTT TTTAGCTGCT 
CTTTATGTCC ATCTAACATT CACCAACTCT GGTATCAACC TGCTGAAAGA AGAAGAAACA 
GCCTCAGGAC AGACTGACGA GTCTTCAGTA AGACAGTTCT ACCAGAGTGC TGTGAACGAG 
GCTTTGAAGA GTTCAAATGG ACACTTGGAC TTGTTTCTTC GCTTCCTCCT TGGTCTTTCG 
ATGCCATCCA ATCAGGATCT CCTACAAGGC CTGCACACAC AAACAGGAAA CAGGTCACAG 
ATAAATCAGG AAGCAGTCAA GTACATTAAA GAAACAATGA ATGACACTCT CTCTTCAGAG 
AAAAGCATCA ATCTGCTCCA CTGTCTGAAT GAATTGAATG ATGACTCTAT AGTGAAGGAG 
GTCCAGCATC ACCTGAGTTC AGGACACCTG TCTAAAGTTA ATCTCTCTCC TGCTCAGTGG 
TCAGCTCTGG TCTTCATCTT ACTGTCATCA GAAACAGGTG TGGATGTGTT TGACTTGAGG 
AAATACTCAG CTTCAGAAGA GGCTCTTCTG CAGCTGCTGC CAGTGGTCAA AGTCTGTAAG 
AAAGCCCAGT TGAGTGGCTG TAACCTCACA GACAGAAGCT GTGAAGCTCT TTCCTCAATT 
CTAAGTTCTC AGTCCTCCAG ACTGAGAGAG CTGGACATGA GTAACAACAA CCTCCAGGAC 
TCAGGAGTGA AGCTGCTTTG TGTTGGACTT GGGAGTCCAC ACTGTATGCT GGAAACTCTC 
AGGCTGTCAG GCTGTCAGAT CACAGGAACA GGCTTCACCT CTCTGGCCAC AGCTCTGTGC 
TCCAACCCCT CCTATTTGAA AGAGCTGGAC TTGAGCTATA ATCACCCAGG AGACTCAGGA 
ATGAAGCTTT TGTCTGCTCT AAAGGAGGAT CCACATGTGA AACTCGACAT ACTCTGTGTG 
GAACCTCAAG GAGTCCAGTG GATGAGACCA GGTCTAAAGA AATATTTCTG TGATCTGACG 
CTGGATCCAA ACACAGCACA CAGAAACCTC AAACTGTCTA AAAACAACAA AGAGGTGACA 
CATGTGAAAG AAGACCAGCT GTATCCTGAT CATGATCATA GGTTTGATCA GTGGCCTCAG 
CTGCTGTGTA CCAACAGTCT GAGTGGTCGC TGTTACTGGG AGGTTGAGTG GAGTGGAGAG 
GTTCATGTTG CTGTAGTTTA TGGAGGAATC AGACGGAAAG GAGAAGAGGA TGAAAGCAGG 
TTTGGAAGGA CTGATCAATC CTGGAGTCTC AACTGCAGTG ATAATAAGGG TTACTATGCA 
GGTCACAAAT TTGATTTAAG AGCCATCCCT CTGCTCTCCT CAGCTCTTTC TCACAAAGTA 
GCAGTGTATG TGGACTGTTC TGCAGGAACT GTGTCTTTTT ACAGTGTCTC CTGTGATAAA 
CTGATCCACC TGCACACCTT CAACACCACC TTCACTGAAC CTGTATATGC TGGGTTTAGA 
GTGAAGCCTG ACTCTAGTAT CCACCTCTGT GCTGACTCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102076273 ( XM_019347731.1 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_019203276.1 Oreochromis niloticus protein NLRC3 (LOC102076273), transcript variant X1, mRNA.

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CloneID	OOr46340
Clone ID Related Accession (Same CDS sequence)	XM_019347732.1 , XM_019347732.2 , XM_019347731.1 , XM_019347733.2 , XM_019347733.1 , XM_019347731.2
Accession Version	XM_019347731.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	protein NLRC3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031982.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAC CAGACTCTCC TGAGCCTGAG ACCGGACGCA GATCTCCACA GAAAGACCGT 
CCTGACTTTA AAATCAGGAA ACTGACTCCT GATGAGAGGA TCACTGAGAC ACCAGATGCC 
CTTGAATCTG AACCTGGACC CAGCTGTGTG TCTCTGAAGA GTGATCAGTC AAAGGATGGC 
ATGATCCTCT TTAAAGGAAT CAAATCTGCT TCTGCTGAGA AAGCTCATCA GGAGAGTCTG 
CAGCATCAAA CTGATCTGGA CTCAGTATTT AAGATGCTGG AGGAAAACAT TATCACGTTT 
GTGAAGAACG AGCTGAAGAA GATGCAGAAT GATCTAAATC CAGATTATTC AGAGTATCAG 
AGGGATGAAG AGGAGGTGTT GGACAGTGAG GAAGAGAAGC AGAGAAGGAG CAGCAGAGAG 
GCATTTCTGA AACTTGCAGT TAACTTCCTG AGAATAATGA AACAGGAGCA GCTGGCTGAC 
TGTCTGCAGA GCAAAAATCC TGATCCACTT TGTGAGAACA CATTTAAATG TAACCTAAAG 
AAGAAGTTCC AGTGTGTCTC TGAGGGGATC GCTAAACCAG GAACCCGAAC CACTCTAAAT 
GAGATCTACA CAGAGCTCTG CATCACAGAG GGAGGGACCG GAGAGGTCAA CACTGAACAT 
GAGGTCAGAC AGATTGAAAT GTTTTCCTGG AAACCAAACA GACCAGAAAC ATCAATCAGG 
TGTGAAGACA TCTTTAAAGC TTCGACTGAA GGAGACAAAC CAATCAGAAC AGTTCTGACC 
AAAGGAGTGG CTGGCATCGG GAAAACACTC CTGACACAAA AGTTCACTTT GGACTGGGCT 
GAAGACAAGA CCAACCAGGA CATCCAGTTC ATTTTTCCAT TCACATTCAG AGAGCTGAAT 
TTACTGAAGG ATAAAAAGTT CAGCTTAGTG GGATTTATTC ATCACTTCTT CACTGAAATC 
AAAGAAGCAG GAATCTGTAA CTTTAAAAAG TTCAAAGTTG TGTTCATCTT TGATGGTCTG 
GATGAGTGTC GACTTCCTCT GAACTTCCAC ATCACTGAGA TCCTCACTGA TGTTACAGAG 
TCCACATCAG TGGATGTGCT GCTGACAAAC CTCATCAGGG GAAAGTTGCT TCCTTCTGCT 
CACATCTGGA TAACCACACG ACCTGCAGCA GCCAATCAGA TCCCTGCTGA CTATATAGAC 
ATGGTTACAG AGGTCAGAGG GTTCACTGAC CCAAAGAAGG AAGAGTACTT TAAGAAGAAA 
CTCAGAGACA AGGAAGAAGC CAACACCATA ATCTCTCACA TCAAGACATC ACGAAGCCTC 
CACATCATGT GTCACATCCC AATCTTCTGC TGGATCACTG CTACAGTTTT AGAGAGTTTG 
TTAAAAACCA AACAGGATGG AGGAAAACTG CCCAAGACAC TGACTGAGAT GTACATTCAC 
TTCCTGGTGG TTCAGACCAA ACTGAAGAAC ATCAAATATG ATGGAGGAGC TGAGACAGAT 
CCTCTCTGGA GTCCAGAGAA CAGAAAGATG ATTGAAGCAC TGGGGAAACT GGCTTTTGAG 
CAGCTGCTGA AAGGAAACCT GATCTTCTAT GAAACAGACC TGACAGAGTG TGGCATTAAT 
GTCAGAGAGG CCTCAGTGTA CTCAGGAGTG TTCACACAGA TTTTTAAAGA GGAGAGAGGA 
CTGTACCAAG AGAAGGTGTT CTGTTTCATC CATCTGAGCA TTCAGGAGTT TTTAGCTGCT 
CTTTATGTCC ATCTAACATT CACCAACTCT GGTATCAACC TGCTGAAAGA AGAAGAAACA 
GCCTCAGGAC AGACTGACGA GTCTTCAGTA AGACAGTTCT ACCAGAGTGC TGTGAACGAG 
GCTTTGAAGA GTTCAAATGG ACACTTGGAC TTGTTTCTTC GCTTCCTCCT TGGTCTTTCG 
ATGCCATCCA ATCAGGATCT CCTACAAGGC CTGCACACAC AAACAGGAAA CAGGTCACAG 
ATAAATCAGG AAGCAGTCAA GTACATTAAA GAAACAATGA ATGACACTCT CTCTTCAGAG 
AAAAGCATCA ATCTGCTCCA CTGTCTGAAT GAATTGAATG ATGACTCTAT AGTGAAGGAG 
GTCCAGCATC ACCTGAGTTC AGGACACCTG TCTAAAGTTA ATCTCTCTCC TGCTCAGTGG 
TCAGCTCTGG TCTTCATCTT ACTGTCATCA GAAACAGGTG TGGATGTGTT TGACTTGAGG 
AAATACTCAG CTTCAGAAGA GGCTCTTCTG CAGCTGCTGC CAGTGGTCAA AGTCTGTAAG 
AAAGCCCAGT TGAGTGGCTG TAACCTCACA GACAGAAGCT GTGAAGCTCT TTCCTCAATT 
CTAAGTTCTC AGTCCTCCAG ACTGAGAGAG CTGGACATGA GTAACAACAA CCTCCAGGAC 
TCAGGAGTGA AGCTGCTTTG TGTTGGACTT GGGAGTCCAC ACTGTATGCT GGAAACTCTC 
AGGCTGTCAG GCTGTCAGAT CACAGGAACA GGCTTCACCT CTCTGGCCAC AGCTCTGTGC 
TCCAACCCCT CCTATTTGAA AGAGCTGGAC TTGAGCTATA ATCACCCAGG AGACTCAGGA 
ATGAAGCTTT TGTCTGCTCT AAAGGAGGAT CCACATGTGA AACTCGACAT ACTCTGTGTG 
GAACCTCAAG GAGTCCAGTG GATGAGACCA GGTCTAAAGA AATATTTCTG TGATCTGACG 
CTGGATCCAA ACACAGCACA CAGAAACCTC AAACTGTCTA AAAACAACAA AGAGGTGACA 
CATGTGAAAG AAGACCAGCT GTATCCTGAT CATGATCATA GGTTTGATCA GTGGCCTCAG 
CTGCTGTGTA CCAACAGTCT GAGTGGTCGC TGTTACTGGG AGGTTGAGTG GAGTGGAGAG 
GTTCATGTTG CTGTAGTTTA TGGAGGAATC AGACGGAAAG GAGAAGAGGA TGAAAGCAGG 
TTTGGAAGGA CTGATCAATC CTGGAGTCTC AACTGCAGTG ATAATAAGGG TTACTATGCA 
GGTCACAAAT TTGATTTAAG AGCCATCCCT CTGCTCTCCT CAGCTCTTTC TCACAAAGTA 
GCAGTGTATG TGGACTGTTC TGCAGGAACT GTGTCTTTTT ACAGTGTCTC CTGTGATAAA 
CTGATCCACC TGCACACCTT CAACACCACC TTCACTGAAC CTGTATATGC TGGGTTTAGA 
GTGAAGCCTG ACTCTAGTAT CCACCTCTGT GCTGACTCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019203276.1
CDS	217..3441
Translation	MERPDSPEPETGRRSPQKDRPDFKIRKLTPDERITETPDALESEPGPSCVSLKSDQSKDGMILFKGIKSASAEKAHQESLQHQTDLDSVFKMLEENIITFVKNELKKMQNDLNPDYSEYQRDEEEVLDSEEEKQRRSSREAFLKLAVNFLRIMKQEQLADCLQSKNPDPLCENTFKCNLKKKFQCVSEGIAKPGTRTTLNEIYTELCITEGGTGEVNTEHEVRQIEMFSWKPNRPETSIRCEDIFKASTEGDKPIRTVLTKGVAGIGKTLLTQKFTLDWAEDKTNQDIQFIFPFTFRELNLLKDKKFSLVGFIHHFFTEIKEAGICNFKKFKVVFIFDGLDECRLPLNFHITEILTDVTESTSVDVLLTNLIRGKLLPSAHIWITTRPAAANQIPADYIDMVTEVRGFTDPKKEEYFKKKLRDKEEANTIISHIKTSRSLHIMCHIPIFCWITATVLESLLKTKQDGGKLPKTLTEMYIHFLVVQTKLKNIKYDGGAETDPLWSPENRKMIEALGKLAFEQLLKGNLIFYETDLTECGINVREASVYSGVFTQIFKEERGLYQEKVFCFIHLSIQEFLAALYVHLTFTNSGINLLKEEETASGQTDESSVRQFYQSAVNEALKSSNGHLDLFLRFLLGLSMPSNQDLLQGLHTQTGNRSQINQEAVKYIKETMNDTLSSEKSINLLHCLNELNDDSIVKEVQHHLSSGHLSKVNLSPAQWSALVFILLSSETGVDVFDLRKYSASEEALLQLLPVVKVCKKAQLSGCNLTDRSCEALSSILSSQSSRLRELDMSNNNLQDSGVKLLCVGLGSPHCMLETLRLSGCQITGTGFTSLATALCSNPSYLKELDLSYNHPGDSGMKLLSALKEDPHVKLDILCVEPQGVQWMRPGLKKYFCDLTLDPNTAHRNLKLSKNNKEVTHVKEDQLYPDHDHRFDQWPQLLCTNSLSGRCYWEVEWSGEVHVAVVYGGIRRKGEEDESRFGRTDQSWSLNCSDNKGYYAGHKFDLRAIPLLSSALSHKVAVYVDCSAGTVSFYSVSCDKLIHLHTFNTTFTEPVYAGFRVKPDSSIHLCADSE

Target ORF information:

RefSeq Version	XM_019347731.1
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus protein NLRC3 (LOC102076273), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019347731.1

ORF Insert Sequence:

ATGGAAAGAC CAGACTCTCC TGAGCCTGAG ACCGGACGCA GATCTCCACA GAAAGACCGT 
CCTGACTTTA AAATCAGGAA ACTGACTCCT GATGAGAGGA TCACTGAGAC ACCAGATGCC 
CTTGAATCTG AACCTGGACC CAGCTGTGTG TCTCTGAAGA GTGATCAGTC AAAGGATGGC 
ATGATCCTCT TTAAAGGAAT CAAATCTGCT TCTGCTGAGA AAGCTCATCA GGAGAGTCTG 
CAGCATCAAA CTGATCTGGA CTCAGTATTT AAGATGCTGG AGGAAAACAT TATCACGTTT 
GTGAAGAACG AGCTGAAGAA GATGCAGAAT GATCTAAATC CAGATTATTC AGAGTATCAG 
AGGGATGAAG AGGAGGTGTT GGACAGTGAG GAAGAGAAGC AGAGAAGGAG CAGCAGAGAG 
GCATTTCTGA AACTTGCAGT TAACTTCCTG AGAATAATGA AACAGGAGCA GCTGGCTGAC 
TGTCTGCAGA GCAAAAATCC TGATCCACTT TGTGAGAACA CATTTAAATG TAACCTAAAG 
AAGAAGTTCC AGTGTGTCTC TGAGGGGATC GCTAAACCAG GAACCCGAAC CACTCTAAAT 
GAGATCTACA CAGAGCTCTG CATCACAGAG GGAGGGACCG GAGAGGTCAA CACTGAACAT 
GAGGTCAGAC AGATTGAAAT GTTTTCCTGG AAACCAAACA GACCAGAAAC ATCAATCAGG 
TGTGAAGACA TCTTTAAAGC TTCGACTGAA GGAGACAAAC CAATCAGAAC AGTTCTGACC 
AAAGGAGTGG CTGGCATCGG GAAAACACTC CTGACACAAA AGTTCACTTT GGACTGGGCT 
GAAGACAAGA CCAACCAGGA CATCCAGTTC ATTTTTCCAT TCACATTCAG AGAGCTGAAT 
TTACTGAAGG ATAAAAAGTT CAGCTTAGTG GGATTTATTC ATCACTTCTT CACTGAAATC 
AAAGAAGCAG GAATCTGTAA CTTTAAAAAG TTCAAAGTTG TGTTCATCTT TGATGGTCTG 
GATGAGTGTC GACTTCCTCT GAACTTCCAC ATCACTGAGA TCCTCACTGA TGTTACAGAG 
TCCACATCAG TGGATGTGCT GCTGACAAAC CTCATCAGGG GAAAGTTGCT TCCTTCTGCT 
CACATCTGGA TAACCACACG ACCTGCAGCA GCCAATCAGA TCCCTGCTGA CTATATAGAC 
ATGGTTACAG AGGTCAGAGG GTTCACTGAC CCAAAGAAGG AAGAGTACTT TAAGAAGAAA 
CTCAGAGACA AGGAAGAAGC CAACACCATA ATCTCTCACA TCAAGACATC ACGAAGCCTC 
CACATCATGT GTCACATCCC AATCTTCTGC TGGATCACTG CTACAGTTTT AGAGAGTTTG 
TTAAAAACCA AACAGGATGG AGGAAAACTG CCCAAGACAC TGACTGAGAT GTACATTCAC 
TTCCTGGTGG TTCAGACCAA ACTGAAGAAC ATCAAATATG ATGGAGGAGC TGAGACAGAT 
CCTCTCTGGA GTCCAGAGAA CAGAAAGATG ATTGAAGCAC TGGGGAAACT GGCTTTTGAG 
CAGCTGCTGA AAGGAAACCT GATCTTCTAT GAAACAGACC TGACAGAGTG TGGCATTAAT 
GTCAGAGAGG CCTCAGTGTA CTCAGGAGTG TTCACACAGA TTTTTAAAGA GGAGAGAGGA 
CTGTACCAAG AGAAGGTGTT CTGTTTCATC CATCTGAGCA TTCAGGAGTT TTTAGCTGCT 
CTTTATGTCC ATCTAACATT CACCAACTCT GGTATCAACC TGCTGAAAGA AGAAGAAACA 
GCCTCAGGAC AGACTGACGA GTCTTCAGTA AGACAGTTCT ACCAGAGTGC TGTGAACGAG 
GCTTTGAAGA GTTCAAATGG ACACTTGGAC TTGTTTCTTC GCTTCCTCCT TGGTCTTTCG 
ATGCCATCCA ATCAGGATCT CCTACAAGGC CTGCACACAC AAACAGGAAA CAGGTCACAG 
ATAAATCAGG AAGCAGTCAA GTACATTAAA GAAACAATGA ATGACACTCT CTCTTCAGAG 
AAAAGCATCA ATCTGCTCCA CTGTCTGAAT GAATTGAATG ATGACTCTAT AGTGAAGGAG 
GTCCAGCATC ACCTGAGTTC AGGACACCTG TCTAAAGTTA ATCTCTCTCC TGCTCAGTGG 
TCAGCTCTGG TCTTCATCTT ACTGTCATCA GAAACAGGTG TGGATGTGTT TGACTTGAGG 
AAATACTCAG CTTCAGAAGA GGCTCTTCTG CAGCTGCTGC CAGTGGTCAA AGTCTGTAAG 
AAAGCCCAGT TGAGTGGCTG TAACCTCACA GACAGAAGCT GTGAAGCTCT TTCCTCAATT 
CTAAGTTCTC AGTCCTCCAG ACTGAGAGAG CTGGACATGA GTAACAACAA CCTCCAGGAC 
TCAGGAGTGA AGCTGCTTTG TGTTGGACTT GGGAGTCCAC ACTGTATGCT GGAAACTCTC 
AGGCTGTCAG GCTGTCAGAT CACAGGAACA GGCTTCACCT CTCTGGCCAC AGCTCTGTGC 
TCCAACCCCT CCTATTTGAA AGAGCTGGAC TTGAGCTATA ATCACCCAGG AGACTCAGGA 
ATGAAGCTTT TGTCTGCTCT AAAGGAGGAT CCACATGTGA AACTCGACAT ACTCTGTGTG 
GAACCTCAAG GAGTCCAGTG GATGAGACCA GGTCTAAAGA AATATTTCTG TGATCTGACG 
CTGGATCCAA ACACAGCACA CAGAAACCTC AAACTGTCTA AAAACAACAA AGAGGTGACA 
CATGTGAAAG AAGACCAGCT GTATCCTGAT CATGATCATA GGTTTGATCA GTGGCCTCAG 
CTGCTGTGTA CCAACAGTCT GAGTGGTCGC TGTTACTGGG AGGTTGAGTG GAGTGGAGAG 
GTTCATGTTG CTGTAGTTTA TGGAGGAATC AGACGGAAAG GAGAAGAGGA TGAAAGCAGG 
TTTGGAAGGA CTGATCAATC CTGGAGTCTC AACTGCAGTG ATAATAAGGG TTACTATGCA 
GGTCACAAAT TTGATTTAAG AGCCATCCCT CTGCTCTCCT CAGCTCTTTC TCACAAAGTA 
GCAGTGTATG TGGACTGTTC TGCAGGAACT GTGTCTTTTT ACAGTGTCTC CTGTGATAAA 
CTGATCCACC TGCACACCTT CAACACCACC TTCACTGAAC CTGTATATGC TGGGTTTAGA 
GTGAAGCCTG ACTCTAGTAT CCACCTCTGT GCTGACTCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102076273 ( XM_019347733.2 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_019203278.1 Oreochromis niloticus NLR family CARD domain-containing protein 3 (LOC102076273), transcript variant X3, mRNA.

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CloneID	OOr46340
Clone ID Related Accession (Same CDS sequence)	XM_019347732.1 , XM_019347732.2 , XM_019347731.1 , XM_019347733.2 , XM_019347733.1 , XM_019347731.2
Accession Version	XM_019347733.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	NLR family CARD domain-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031982.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_019347733.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAC CAGACTCTCC TGAGCCTGAG ACCGGACGCA GATCTCCACA GAAAGACCGT 
CCTGACTTTA AAATCAGGAA ACTGACTCCT GATGAGAGGA TCACTGAGAC ACCAGATGCC 
CTTGAATCTG AACCTGGACC CAGCTGTGTG TCTCTGAAGA GTGATCAGTC AAAGGATGGC 
ATGATCCTCT TTAAAGGAAT CAAATCTGCT TCTGCTGAGA AAGCTCATCA GGAGAGTCTG 
CAGCATCAAA CTGATCTGGA CTCAGTATTT AAGATGCTGG AGGAAAACAT TATCACGTTT 
GTGAAGAACG AGCTGAAGAA GATGCAGAAT GATCTAAATC CAGATTATTC AGAGTATCAG 
AGGGATGAAG AGGAGGTGTT GGACAGTGAG GAAGAGAAGC AGAGAAGGAG CAGCAGAGAG 
GCATTTCTGA AACTTGCAGT TAACTTCCTG AGAATAATGA AACAGGAGCA GCTGGCTGAC 
TGTCTGCAGA GCAAAAATCC TGATCCACTT TGTGAGAACA CATTTAAATG TAACCTAAAG 
AAGAAGTTCC AGTGTGTCTC TGAGGGGATC GCTAAACCAG GAACCCGAAC CACTCTAAAT 
GAGATCTACA CAGAGCTCTG CATCACAGAG GGAGGGACCG GAGAGGTCAA CACTGAACAT 
GAGGTCAGAC AGATTGAAAT GTTTTCCTGG AAACCAAACA GACCAGAAAC ATCAATCAGG 
TGTGAAGACA TCTTTAAAGC TTCGACTGAA GGAGACAAAC CAATCAGAAC AGTTCTGACC 
AAAGGAGTGG CTGGCATCGG GAAAACACTC CTGACACAAA AGTTCACTTT GGACTGGGCT 
GAAGACAAGA CCAACCAGGA CATCCAGTTC ATTTTTCCAT TCACATTCAG AGAGCTGAAT 
TTACTGAAGG ATAAAAAGTT CAGCTTAGTG GGATTTATTC ATCACTTCTT CACTGAAATC 
AAAGAAGCAG GAATCTGTAA CTTTAAAAAG TTCAAAGTTG TGTTCATCTT TGATGGTCTG 
GATGAGTGTC GACTTCCTCT GAACTTCCAC ATCACTGAGA TCCTCACTGA TGTTACAGAG 
TCCACATCAG TGGATGTGCT GCTGACAAAC CTCATCAGGG GAAAGTTGCT TCCTTCTGCT 
CACATCTGGA TAACCACACG ACCTGCAGCA GCCAATCAGA TCCCTGCTGA CTATATAGAC 
ATGGTTACAG AGGTCAGAGG GTTCACTGAC CCAAAGAAGG AAGAGTACTT TAAGAAGAAA 
CTCAGAGACA AGGAAGAAGC CAACACCATA ATCTCTCACA TCAAGACATC ACGAAGCCTC 
CACATCATGT GTCACATCCC AATCTTCTGC TGGATCACTG CTACAGTTTT AGAGAGTTTG 
TTAAAAACCA AACAGGATGG AGGAAAACTG CCCAAGACAC TGACTGAGAT GTACATTCAC 
TTCCTGGTGG TTCAGACCAA ACTGAAGAAC ATCAAATATG ATGGAGGAGC TGAGACAGAT 
CCTCTCTGGA GTCCAGAGAA CAGAAAGATG ATTGAAGCAC TGGGGAAACT GGCTTTTGAG 
CAGCTGCTGA AAGGAAACCT GATCTTCTAT GAAACAGACC TGACAGAGTG TGGCATTAAT 
GTCAGAGAGG CCTCAGTGTA CTCAGGAGTG TTCACACAGA TTTTTAAAGA GGAGAGAGGA 
CTGTACCAAG AGAAGGTGTT CTGTTTCATC CATCTGAGCA TTCAGGAGTT TTTAGCTGCT 
CTTTATGTCC ATCTAACATT CACCAACTCT GGTATCAACC TGCTGAAAGA AGAAGAAACA 
GCCTCAGGAC AGACTGACGA GTCTTCAGTA AGACAGTTCT ACCAGAGTGC TGTGAACGAG 
GCTTTGAAGA GTTCAAATGG ACACTTGGAC TTGTTTCTTC GCTTCCTCCT TGGTCTTTCG 
ATGCCATCCA ATCAGGATCT CCTACAAGGC CTGCACACAC AAACAGGAAA CAGGTCACAG 
ATAAATCAGG AAGCAGTCAA GTACATTAAA GAAACAATGA ATGACACTCT CTCTTCAGAG 
AAAAGCATCA ATCTGCTCCA CTGTCTGAAT GAATTGAATG ATGACTCTAT AGTGAAGGAG 
GTCCAGCATC ACCTGAGTTC AGGACACCTG TCTAAAGTTA ATCTCTCTCC TGCTCAGTGG 
TCAGCTCTGG TCTTCATCTT ACTGTCATCA GAAACAGGTG TGGATGTGTT TGACTTGAGG 
AAATACTCAG CTTCAGAAGA GGCTCTTCTG CAGCTGCTGC CAGTGGTCAA AGTCTGTAAG 
AAAGCCCAGT TGAGTGGCTG TAACCTCACA GACAGAAGCT GTGAAGCTCT TTCCTCAATT 
CTAAGTTCTC AGTCCTCCAG ACTGAGAGAG CTGGACATGA GTAACAACAA CCTCCAGGAC 
TCAGGAGTGA AGCTGCTTTG TGTTGGACTT GGGAGTCCAC ACTGTATGCT GGAAACTCTC 
AGGCTGTCAG GCTGTCAGAT CACAGGAACA GGCTTCACCT CTCTGGCCAC AGCTCTGTGC 
TCCAACCCCT CCTATTTGAA AGAGCTGGAC TTGAGCTATA ATCACCCAGG AGACTCAGGA 
ATGAAGCTTT TGTCTGCTCT AAAGGAGGAT CCACATGTGA AACTCGACAT ACTCTGTGTG 
GAACCTCAAG GAGTCCAGTG GATGAGACCA GGTCTAAAGA AATATTTCTG TGATCTGACG 
CTGGATCCAA ACACAGCACA CAGAAACCTC AAACTGTCTA AAAACAACAA AGAGGTGACA 
CATGTGAAAG AAGACCAGCT GTATCCTGAT CATGATCATA GGTTTGATCA GTGGCCTCAG 
CTGCTGTGTA CCAACAGTCT GAGTGGTCGC TGTTACTGGG AGGTTGAGTG GAGTGGAGAG 
GTTCATGTTG CTGTAGTTTA TGGAGGAATC AGACGGAAAG GAGAAGAGGA TGAAAGCAGG 
TTTGGAAGGA CTGATCAATC CTGGAGTCTC AACTGCAGTG ATAATAAGGG TTACTATGCA 
GGTCACAAAT TTGATTTAAG AGCCATCCCT CTGCTCTCCT CAGCTCTTTC TCACAAAGTA 
GCAGTGTATG TGGACTGTTC TGCAGGAACT GTGTCTTTTT ACAGTGTCTC CTGTGATAAA 
CTGATCCACC TGCACACCTT CAACACCACC TTCACTGAAC CTGTATATGC TGGGTTTAGA 
GTGAAGCCTG ACTCTAGTAT CCACCTCTGT GCTGACTCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019203278.1
CDS	450..3674
Translation	MERPDSPEPETGRRSPQKDRPDFKIRKLTPDERITETPDALESEPGPSCVSLKSDQSKDGMILFKGIKSASAEKAHQESLQHQTDLDSVFKMLEENIITFVKNELKKMQNDLNPDYSEYQRDEEEVLDSEEEKQRRSSREAFLKLAVNFLRIMKQEQLADCLQSKNPDPLCENTFKCNLKKKFQCVSEGIAKPGTRTTLNEIYTELCITEGGTGEVNTEHEVRQIEMFSWKPNRPETSIRCEDIFKASTEGDKPIRTVLTKGVAGIGKTLLTQKFTLDWAEDKTNQDIQFIFPFTFRELNLLKDKKFSLVGFIHHFFTEIKEAGICNFKKFKVVFIFDGLDECRLPLNFHITEILTDVTESTSVDVLLTNLIRGKLLPSAHIWITTRPAAANQIPADYIDMVTEVRGFTDPKKEEYFKKKLRDKEEANTIISHIKTSRSLHIMCHIPIFCWITATVLESLLKTKQDGGKLPKTLTEMYIHFLVVQTKLKNIKYDGGAETDPLWSPENRKMIEALGKLAFEQLLKGNLIFYETDLTECGINVREASVYSGVFTQIFKEERGLYQEKVFCFIHLSIQEFLAALYVHLTFTNSGINLLKEEETASGQTDESSVRQFYQSAVNEALKSSNGHLDLFLRFLLGLSMPSNQDLLQGLHTQTGNRSQINQEAVKYIKETMNDTLSSEKSINLLHCLNELNDDSIVKEVQHHLSSGHLSKVNLSPAQWSALVFILLSSETGVDVFDLRKYSASEEALLQLLPVVKVCKKAQLSGCNLTDRSCEALSSILSSQSSRLRELDMSNNNLQDSGVKLLCVGLGSPHCMLETLRLSGCQITGTGFTSLATALCSNPSYLKELDLSYNHPGDSGMKLLSALKEDPHVKLDILCVEPQGVQWMRPGLKKYFCDLTLDPNTAHRNLKLSKNNKEVTHVKEDQLYPDHDHRFDQWPQLLCTNSLSGRCYWEVEWSGEVHVAVVYGGIRRKGEEDESRFGRTDQSWSLNCSDNKGYYAGHKFDLRAIPLLSSALSHKVAVYVDCSAGTVSFYSVSCDKLIHLHTFNTTFTEPVYAGFRVKPDSSIHLCADSE

Target ORF information:

RefSeq Version	XM_019347733.2
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus NLR family CARD domain-containing protein 3 (LOC102076273), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019347733.2

ORF Insert Sequence:

ATGGAAAGAC CAGACTCTCC TGAGCCTGAG ACCGGACGCA GATCTCCACA GAAAGACCGT 
CCTGACTTTA AAATCAGGAA ACTGACTCCT GATGAGAGGA TCACTGAGAC ACCAGATGCC 
CTTGAATCTG AACCTGGACC CAGCTGTGTG TCTCTGAAGA GTGATCAGTC AAAGGATGGC 
ATGATCCTCT TTAAAGGAAT CAAATCTGCT TCTGCTGAGA AAGCTCATCA GGAGAGTCTG 
CAGCATCAAA CTGATCTGGA CTCAGTATTT AAGATGCTGG AGGAAAACAT TATCACGTTT 
GTGAAGAACG AGCTGAAGAA GATGCAGAAT GATCTAAATC CAGATTATTC AGAGTATCAG 
AGGGATGAAG AGGAGGTGTT GGACAGTGAG GAAGAGAAGC AGAGAAGGAG CAGCAGAGAG 
GCATTTCTGA AACTTGCAGT TAACTTCCTG AGAATAATGA AACAGGAGCA GCTGGCTGAC 
TGTCTGCAGA GCAAAAATCC TGATCCACTT TGTGAGAACA CATTTAAATG TAACCTAAAG 
AAGAAGTTCC AGTGTGTCTC TGAGGGGATC GCTAAACCAG GAACCCGAAC CACTCTAAAT 
GAGATCTACA CAGAGCTCTG CATCACAGAG GGAGGGACCG GAGAGGTCAA CACTGAACAT 
GAGGTCAGAC AGATTGAAAT GTTTTCCTGG AAACCAAACA GACCAGAAAC ATCAATCAGG 
TGTGAAGACA TCTTTAAAGC TTCGACTGAA GGAGACAAAC CAATCAGAAC AGTTCTGACC 
AAAGGAGTGG CTGGCATCGG GAAAACACTC CTGACACAAA AGTTCACTTT GGACTGGGCT 
GAAGACAAGA CCAACCAGGA CATCCAGTTC ATTTTTCCAT TCACATTCAG AGAGCTGAAT 
TTACTGAAGG ATAAAAAGTT CAGCTTAGTG GGATTTATTC ATCACTTCTT CACTGAAATC 
AAAGAAGCAG GAATCTGTAA CTTTAAAAAG TTCAAAGTTG TGTTCATCTT TGATGGTCTG 
GATGAGTGTC GACTTCCTCT GAACTTCCAC ATCACTGAGA TCCTCACTGA TGTTACAGAG 
TCCACATCAG TGGATGTGCT GCTGACAAAC CTCATCAGGG GAAAGTTGCT TCCTTCTGCT 
CACATCTGGA TAACCACACG ACCTGCAGCA GCCAATCAGA TCCCTGCTGA CTATATAGAC 
ATGGTTACAG AGGTCAGAGG GTTCACTGAC CCAAAGAAGG AAGAGTACTT TAAGAAGAAA 
CTCAGAGACA AGGAAGAAGC CAACACCATA ATCTCTCACA TCAAGACATC ACGAAGCCTC 
CACATCATGT GTCACATCCC AATCTTCTGC TGGATCACTG CTACAGTTTT AGAGAGTTTG 
TTAAAAACCA AACAGGATGG AGGAAAACTG CCCAAGACAC TGACTGAGAT GTACATTCAC 
TTCCTGGTGG TTCAGACCAA ACTGAAGAAC ATCAAATATG ATGGAGGAGC TGAGACAGAT 
CCTCTCTGGA GTCCAGAGAA CAGAAAGATG ATTGAAGCAC TGGGGAAACT GGCTTTTGAG 
CAGCTGCTGA AAGGAAACCT GATCTTCTAT GAAACAGACC TGACAGAGTG TGGCATTAAT 
GTCAGAGAGG CCTCAGTGTA CTCAGGAGTG TTCACACAGA TTTTTAAAGA GGAGAGAGGA 
CTGTACCAAG AGAAGGTGTT CTGTTTCATC CATCTGAGCA TTCAGGAGTT TTTAGCTGCT 
CTTTATGTCC ATCTAACATT CACCAACTCT GGTATCAACC TGCTGAAAGA AGAAGAAACA 
GCCTCAGGAC AGACTGACGA GTCTTCAGTA AGACAGTTCT ACCAGAGTGC TGTGAACGAG 
GCTTTGAAGA GTTCAAATGG ACACTTGGAC TTGTTTCTTC GCTTCCTCCT TGGTCTTTCG 
ATGCCATCCA ATCAGGATCT CCTACAAGGC CTGCACACAC AAACAGGAAA CAGGTCACAG 
ATAAATCAGG AAGCAGTCAA GTACATTAAA GAAACAATGA ATGACACTCT CTCTTCAGAG 
AAAAGCATCA ATCTGCTCCA CTGTCTGAAT GAATTGAATG ATGACTCTAT AGTGAAGGAG 
GTCCAGCATC ACCTGAGTTC AGGACACCTG TCTAAAGTTA ATCTCTCTCC TGCTCAGTGG 
TCAGCTCTGG TCTTCATCTT ACTGTCATCA GAAACAGGTG TGGATGTGTT TGACTTGAGG 
AAATACTCAG CTTCAGAAGA GGCTCTTCTG CAGCTGCTGC CAGTGGTCAA AGTCTGTAAG 
AAAGCCCAGT TGAGTGGCTG TAACCTCACA GACAGAAGCT GTGAAGCTCT TTCCTCAATT 
CTAAGTTCTC AGTCCTCCAG ACTGAGAGAG CTGGACATGA GTAACAACAA CCTCCAGGAC 
TCAGGAGTGA AGCTGCTTTG TGTTGGACTT GGGAGTCCAC ACTGTATGCT GGAAACTCTC 
AGGCTGTCAG GCTGTCAGAT CACAGGAACA GGCTTCACCT CTCTGGCCAC AGCTCTGTGC 
TCCAACCCCT CCTATTTGAA AGAGCTGGAC TTGAGCTATA ATCACCCAGG AGACTCAGGA 
ATGAAGCTTT TGTCTGCTCT AAAGGAGGAT CCACATGTGA AACTCGACAT ACTCTGTGTG 
GAACCTCAAG GAGTCCAGTG GATGAGACCA GGTCTAAAGA AATATTTCTG TGATCTGACG 
CTGGATCCAA ACACAGCACA CAGAAACCTC AAACTGTCTA AAAACAACAA AGAGGTGACA 
CATGTGAAAG AAGACCAGCT GTATCCTGAT CATGATCATA GGTTTGATCA GTGGCCTCAG 
CTGCTGTGTA CCAACAGTCT GAGTGGTCGC TGTTACTGGG AGGTTGAGTG GAGTGGAGAG 
GTTCATGTTG CTGTAGTTTA TGGAGGAATC AGACGGAAAG GAGAAGAGGA TGAAAGCAGG 
TTTGGAAGGA CTGATCAATC CTGGAGTCTC AACTGCAGTG ATAATAAGGG TTACTATGCA 
GGTCACAAAT TTGATTTAAG AGCCATCCCT CTGCTCTCCT CAGCTCTTTC TCACAAAGTA 
GCAGTGTATG TGGACTGTTC TGCAGGAACT GTGTCTTTTT ACAGTGTCTC CTGTGATAAA 
CTGATCCACC TGCACACCTT CAACACCACC TTCACTGAAC CTGTATATGC TGGGTTTAGA 
GTGAAGCCTG ACTCTAGTAT CCACCTCTGT GCTGACTCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102076273 ( XM_019347733.1 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_019203278.1 Oreochromis niloticus protein NLRC3 (LOC102076273), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOr46340
Clone ID Related Accession (Same CDS sequence)	XM_019347732.1 , XM_019347732.2 , XM_019347731.1 , XM_019347733.2 , XM_019347733.1 , XM_019347731.2
Accession Version	XM_019347733.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	protein NLRC3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031982.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAC CAGACTCTCC TGAGCCTGAG ACCGGACGCA GATCTCCACA GAAAGACCGT 
CCTGACTTTA AAATCAGGAA ACTGACTCCT GATGAGAGGA TCACTGAGAC ACCAGATGCC 
CTTGAATCTG AACCTGGACC CAGCTGTGTG TCTCTGAAGA GTGATCAGTC AAAGGATGGC 
ATGATCCTCT TTAAAGGAAT CAAATCTGCT TCTGCTGAGA AAGCTCATCA GGAGAGTCTG 
CAGCATCAAA CTGATCTGGA CTCAGTATTT AAGATGCTGG AGGAAAACAT TATCACGTTT 
GTGAAGAACG AGCTGAAGAA GATGCAGAAT GATCTAAATC CAGATTATTC AGAGTATCAG 
AGGGATGAAG AGGAGGTGTT GGACAGTGAG GAAGAGAAGC AGAGAAGGAG CAGCAGAGAG 
GCATTTCTGA AACTTGCAGT TAACTTCCTG AGAATAATGA AACAGGAGCA GCTGGCTGAC 
TGTCTGCAGA GCAAAAATCC TGATCCACTT TGTGAGAACA CATTTAAATG TAACCTAAAG 
AAGAAGTTCC AGTGTGTCTC TGAGGGGATC GCTAAACCAG GAACCCGAAC CACTCTAAAT 
GAGATCTACA CAGAGCTCTG CATCACAGAG GGAGGGACCG GAGAGGTCAA CACTGAACAT 
GAGGTCAGAC AGATTGAAAT GTTTTCCTGG AAACCAAACA GACCAGAAAC ATCAATCAGG 
TGTGAAGACA TCTTTAAAGC TTCGACTGAA GGAGACAAAC CAATCAGAAC AGTTCTGACC 
AAAGGAGTGG CTGGCATCGG GAAAACACTC CTGACACAAA AGTTCACTTT GGACTGGGCT 
GAAGACAAGA CCAACCAGGA CATCCAGTTC ATTTTTCCAT TCACATTCAG AGAGCTGAAT 
TTACTGAAGG ATAAAAAGTT CAGCTTAGTG GGATTTATTC ATCACTTCTT CACTGAAATC 
AAAGAAGCAG GAATCTGTAA CTTTAAAAAG TTCAAAGTTG TGTTCATCTT TGATGGTCTG 
GATGAGTGTC GACTTCCTCT GAACTTCCAC ATCACTGAGA TCCTCACTGA TGTTACAGAG 
TCCACATCAG TGGATGTGCT GCTGACAAAC CTCATCAGGG GAAAGTTGCT TCCTTCTGCT 
CACATCTGGA TAACCACACG ACCTGCAGCA GCCAATCAGA TCCCTGCTGA CTATATAGAC 
ATGGTTACAG AGGTCAGAGG GTTCACTGAC CCAAAGAAGG AAGAGTACTT TAAGAAGAAA 
CTCAGAGACA AGGAAGAAGC CAACACCATA ATCTCTCACA TCAAGACATC ACGAAGCCTC 
CACATCATGT GTCACATCCC AATCTTCTGC TGGATCACTG CTACAGTTTT AGAGAGTTTG 
TTAAAAACCA AACAGGATGG AGGAAAACTG CCCAAGACAC TGACTGAGAT GTACATTCAC 
TTCCTGGTGG TTCAGACCAA ACTGAAGAAC ATCAAATATG ATGGAGGAGC TGAGACAGAT 
CCTCTCTGGA GTCCAGAGAA CAGAAAGATG ATTGAAGCAC TGGGGAAACT GGCTTTTGAG 
CAGCTGCTGA AAGGAAACCT GATCTTCTAT GAAACAGACC TGACAGAGTG TGGCATTAAT 
GTCAGAGAGG CCTCAGTGTA CTCAGGAGTG TTCACACAGA TTTTTAAAGA GGAGAGAGGA 
CTGTACCAAG AGAAGGTGTT CTGTTTCATC CATCTGAGCA TTCAGGAGTT TTTAGCTGCT 
CTTTATGTCC ATCTAACATT CACCAACTCT GGTATCAACC TGCTGAAAGA AGAAGAAACA 
GCCTCAGGAC AGACTGACGA GTCTTCAGTA AGACAGTTCT ACCAGAGTGC TGTGAACGAG 
GCTTTGAAGA GTTCAAATGG ACACTTGGAC TTGTTTCTTC GCTTCCTCCT TGGTCTTTCG 
ATGCCATCCA ATCAGGATCT CCTACAAGGC CTGCACACAC AAACAGGAAA CAGGTCACAG 
ATAAATCAGG AAGCAGTCAA GTACATTAAA GAAACAATGA ATGACACTCT CTCTTCAGAG 
AAAAGCATCA ATCTGCTCCA CTGTCTGAAT GAATTGAATG ATGACTCTAT AGTGAAGGAG 
GTCCAGCATC ACCTGAGTTC AGGACACCTG TCTAAAGTTA ATCTCTCTCC TGCTCAGTGG 
TCAGCTCTGG TCTTCATCTT ACTGTCATCA GAAACAGGTG TGGATGTGTT TGACTTGAGG 
AAATACTCAG CTTCAGAAGA GGCTCTTCTG CAGCTGCTGC CAGTGGTCAA AGTCTGTAAG 
AAAGCCCAGT TGAGTGGCTG TAACCTCACA GACAGAAGCT GTGAAGCTCT TTCCTCAATT 
CTAAGTTCTC AGTCCTCCAG ACTGAGAGAG CTGGACATGA GTAACAACAA CCTCCAGGAC 
TCAGGAGTGA AGCTGCTTTG TGTTGGACTT GGGAGTCCAC ACTGTATGCT GGAAACTCTC 
AGGCTGTCAG GCTGTCAGAT CACAGGAACA GGCTTCACCT CTCTGGCCAC AGCTCTGTGC 
TCCAACCCCT CCTATTTGAA AGAGCTGGAC TTGAGCTATA ATCACCCAGG AGACTCAGGA 
ATGAAGCTTT TGTCTGCTCT AAAGGAGGAT CCACATGTGA AACTCGACAT ACTCTGTGTG 
GAACCTCAAG GAGTCCAGTG GATGAGACCA GGTCTAAAGA AATATTTCTG TGATCTGACG 
CTGGATCCAA ACACAGCACA CAGAAACCTC AAACTGTCTA AAAACAACAA AGAGGTGACA 
CATGTGAAAG AAGACCAGCT GTATCCTGAT CATGATCATA GGTTTGATCA GTGGCCTCAG 
CTGCTGTGTA CCAACAGTCT GAGTGGTCGC TGTTACTGGG AGGTTGAGTG GAGTGGAGAG 
GTTCATGTTG CTGTAGTTTA TGGAGGAATC AGACGGAAAG GAGAAGAGGA TGAAAGCAGG 
TTTGGAAGGA CTGATCAATC CTGGAGTCTC AACTGCAGTG ATAATAAGGG TTACTATGCA 
GGTCACAAAT TTGATTTAAG AGCCATCCCT CTGCTCTCCT CAGCTCTTTC TCACAAAGTA 
GCAGTGTATG TGGACTGTTC TGCAGGAACT GTGTCTTTTT ACAGTGTCTC CTGTGATAAA 
CTGATCCACC TGCACACCTT CAACACCACC TTCACTGAAC CTGTATATGC TGGGTTTAGA 
GTGAAGCCTG ACTCTAGTAT CCACCTCTGT GCTGACTCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019203278.1
CDS	453..3677
Translation	MERPDSPEPETGRRSPQKDRPDFKIRKLTPDERITETPDALESEPGPSCVSLKSDQSKDGMILFKGIKSASAEKAHQESLQHQTDLDSVFKMLEENIITFVKNELKKMQNDLNPDYSEYQRDEEEVLDSEEEKQRRSSREAFLKLAVNFLRIMKQEQLADCLQSKNPDPLCENTFKCNLKKKFQCVSEGIAKPGTRTTLNEIYTELCITEGGTGEVNTEHEVRQIEMFSWKPNRPETSIRCEDIFKASTEGDKPIRTVLTKGVAGIGKTLLTQKFTLDWAEDKTNQDIQFIFPFTFRELNLLKDKKFSLVGFIHHFFTEIKEAGICNFKKFKVVFIFDGLDECRLPLNFHITEILTDVTESTSVDVLLTNLIRGKLLPSAHIWITTRPAAANQIPADYIDMVTEVRGFTDPKKEEYFKKKLRDKEEANTIISHIKTSRSLHIMCHIPIFCWITATVLESLLKTKQDGGKLPKTLTEMYIHFLVVQTKLKNIKYDGGAETDPLWSPENRKMIEALGKLAFEQLLKGNLIFYETDLTECGINVREASVYSGVFTQIFKEERGLYQEKVFCFIHLSIQEFLAALYVHLTFTNSGINLLKEEETASGQTDESSVRQFYQSAVNEALKSSNGHLDLFLRFLLGLSMPSNQDLLQGLHTQTGNRSQINQEAVKYIKETMNDTLSSEKSINLLHCLNELNDDSIVKEVQHHLSSGHLSKVNLSPAQWSALVFILLSSETGVDVFDLRKYSASEEALLQLLPVVKVCKKAQLSGCNLTDRSCEALSSILSSQSSRLRELDMSNNNLQDSGVKLLCVGLGSPHCMLETLRLSGCQITGTGFTSLATALCSNPSYLKELDLSYNHPGDSGMKLLSALKEDPHVKLDILCVEPQGVQWMRPGLKKYFCDLTLDPNTAHRNLKLSKNNKEVTHVKEDQLYPDHDHRFDQWPQLLCTNSLSGRCYWEVEWSGEVHVAVVYGGIRRKGEEDESRFGRTDQSWSLNCSDNKGYYAGHKFDLRAIPLLSSALSHKVAVYVDCSAGTVSFYSVSCDKLIHLHTFNTTFTEPVYAGFRVKPDSSIHLCADSE

Target ORF information:

RefSeq Version	XM_019347733.1
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus protein NLRC3 (LOC102076273), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019347733.1

ORF Insert Sequence:

ATGGAAAGAC CAGACTCTCC TGAGCCTGAG ACCGGACGCA GATCTCCACA GAAAGACCGT 
CCTGACTTTA AAATCAGGAA ACTGACTCCT GATGAGAGGA TCACTGAGAC ACCAGATGCC 
CTTGAATCTG AACCTGGACC CAGCTGTGTG TCTCTGAAGA GTGATCAGTC AAAGGATGGC 
ATGATCCTCT TTAAAGGAAT CAAATCTGCT TCTGCTGAGA AAGCTCATCA GGAGAGTCTG 
CAGCATCAAA CTGATCTGGA CTCAGTATTT AAGATGCTGG AGGAAAACAT TATCACGTTT 
GTGAAGAACG AGCTGAAGAA GATGCAGAAT GATCTAAATC CAGATTATTC AGAGTATCAG 
AGGGATGAAG AGGAGGTGTT GGACAGTGAG GAAGAGAAGC AGAGAAGGAG CAGCAGAGAG 
GCATTTCTGA AACTTGCAGT TAACTTCCTG AGAATAATGA AACAGGAGCA GCTGGCTGAC 
TGTCTGCAGA GCAAAAATCC TGATCCACTT TGTGAGAACA CATTTAAATG TAACCTAAAG 
AAGAAGTTCC AGTGTGTCTC TGAGGGGATC GCTAAACCAG GAACCCGAAC CACTCTAAAT 
GAGATCTACA CAGAGCTCTG CATCACAGAG GGAGGGACCG GAGAGGTCAA CACTGAACAT 
GAGGTCAGAC AGATTGAAAT GTTTTCCTGG AAACCAAACA GACCAGAAAC ATCAATCAGG 
TGTGAAGACA TCTTTAAAGC TTCGACTGAA GGAGACAAAC CAATCAGAAC AGTTCTGACC 
AAAGGAGTGG CTGGCATCGG GAAAACACTC CTGACACAAA AGTTCACTTT GGACTGGGCT 
GAAGACAAGA CCAACCAGGA CATCCAGTTC ATTTTTCCAT TCACATTCAG AGAGCTGAAT 
TTACTGAAGG ATAAAAAGTT CAGCTTAGTG GGATTTATTC ATCACTTCTT CACTGAAATC 
AAAGAAGCAG GAATCTGTAA CTTTAAAAAG TTCAAAGTTG TGTTCATCTT TGATGGTCTG 
GATGAGTGTC GACTTCCTCT GAACTTCCAC ATCACTGAGA TCCTCACTGA TGTTACAGAG 
TCCACATCAG TGGATGTGCT GCTGACAAAC CTCATCAGGG GAAAGTTGCT TCCTTCTGCT 
CACATCTGGA TAACCACACG ACCTGCAGCA GCCAATCAGA TCCCTGCTGA CTATATAGAC 
ATGGTTACAG AGGTCAGAGG GTTCACTGAC CCAAAGAAGG AAGAGTACTT TAAGAAGAAA 
CTCAGAGACA AGGAAGAAGC CAACACCATA ATCTCTCACA TCAAGACATC ACGAAGCCTC 
CACATCATGT GTCACATCCC AATCTTCTGC TGGATCACTG CTACAGTTTT AGAGAGTTTG 
TTAAAAACCA AACAGGATGG AGGAAAACTG CCCAAGACAC TGACTGAGAT GTACATTCAC 
TTCCTGGTGG TTCAGACCAA ACTGAAGAAC ATCAAATATG ATGGAGGAGC TGAGACAGAT 
CCTCTCTGGA GTCCAGAGAA CAGAAAGATG ATTGAAGCAC TGGGGAAACT GGCTTTTGAG 
CAGCTGCTGA AAGGAAACCT GATCTTCTAT GAAACAGACC TGACAGAGTG TGGCATTAAT 
GTCAGAGAGG CCTCAGTGTA CTCAGGAGTG TTCACACAGA TTTTTAAAGA GGAGAGAGGA 
CTGTACCAAG AGAAGGTGTT CTGTTTCATC CATCTGAGCA TTCAGGAGTT TTTAGCTGCT 
CTTTATGTCC ATCTAACATT CACCAACTCT GGTATCAACC TGCTGAAAGA AGAAGAAACA 
GCCTCAGGAC AGACTGACGA GTCTTCAGTA AGACAGTTCT ACCAGAGTGC TGTGAACGAG 
GCTTTGAAGA GTTCAAATGG ACACTTGGAC TTGTTTCTTC GCTTCCTCCT TGGTCTTTCG 
ATGCCATCCA ATCAGGATCT CCTACAAGGC CTGCACACAC AAACAGGAAA CAGGTCACAG 
ATAAATCAGG AAGCAGTCAA GTACATTAAA GAAACAATGA ATGACACTCT CTCTTCAGAG 
AAAAGCATCA ATCTGCTCCA CTGTCTGAAT GAATTGAATG ATGACTCTAT AGTGAAGGAG 
GTCCAGCATC ACCTGAGTTC AGGACACCTG TCTAAAGTTA ATCTCTCTCC TGCTCAGTGG 
TCAGCTCTGG TCTTCATCTT ACTGTCATCA GAAACAGGTG TGGATGTGTT TGACTTGAGG 
AAATACTCAG CTTCAGAAGA GGCTCTTCTG CAGCTGCTGC CAGTGGTCAA AGTCTGTAAG 
AAAGCCCAGT TGAGTGGCTG TAACCTCACA GACAGAAGCT GTGAAGCTCT TTCCTCAATT 
CTAAGTTCTC AGTCCTCCAG ACTGAGAGAG CTGGACATGA GTAACAACAA CCTCCAGGAC 
TCAGGAGTGA AGCTGCTTTG TGTTGGACTT GGGAGTCCAC ACTGTATGCT GGAAACTCTC 
AGGCTGTCAG GCTGTCAGAT CACAGGAACA GGCTTCACCT CTCTGGCCAC AGCTCTGTGC 
TCCAACCCCT CCTATTTGAA AGAGCTGGAC TTGAGCTATA ATCACCCAGG AGACTCAGGA 
ATGAAGCTTT TGTCTGCTCT AAAGGAGGAT CCACATGTGA AACTCGACAT ACTCTGTGTG 
GAACCTCAAG GAGTCCAGTG GATGAGACCA GGTCTAAAGA AATATTTCTG TGATCTGACG 
CTGGATCCAA ACACAGCACA CAGAAACCTC AAACTGTCTA AAAACAACAA AGAGGTGACA 
CATGTGAAAG AAGACCAGCT GTATCCTGAT CATGATCATA GGTTTGATCA GTGGCCTCAG 
CTGCTGTGTA CCAACAGTCT GAGTGGTCGC TGTTACTGGG AGGTTGAGTG GAGTGGAGAG 
GTTCATGTTG CTGTAGTTTA TGGAGGAATC AGACGGAAAG GAGAAGAGGA TGAAAGCAGG 
TTTGGAAGGA CTGATCAATC CTGGAGTCTC AACTGCAGTG ATAATAAGGG TTACTATGCA 
GGTCACAAAT TTGATTTAAG AGCCATCCCT CTGCTCTCCT CAGCTCTTTC TCACAAAGTA 
GCAGTGTATG TGGACTGTTC TGCAGGAACT GTGTCTTTTT ACAGTGTCTC CTGTGATAAA 
CTGATCCACC TGCACACCTT CAACACCACC TTCACTGAAC CTGTATATGC TGGGTTTAGA 
GTGAAGCCTG ACTCTAGTAT CCACCTCTGT GCTGACTCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102076273 ( XM_019347731.2 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_019203276.1 Oreochromis niloticus NLR family CARD domain-containing protein 3 (LOC102076273), transcript variant X1, mRNA.

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CloneID	OOr46340
Clone ID Related Accession (Same CDS sequence)	XM_019347732.1 , XM_019347732.2 , XM_019347731.1 , XM_019347733.2 , XM_019347733.1 , XM_019347731.2
Accession Version	XM_019347731.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	NLR family CARD domain-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031982.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_019347731.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAC CAGACTCTCC TGAGCCTGAG ACCGGACGCA GATCTCCACA GAAAGACCGT 
CCTGACTTTA AAATCAGGAA ACTGACTCCT GATGAGAGGA TCACTGAGAC ACCAGATGCC 
CTTGAATCTG AACCTGGACC CAGCTGTGTG TCTCTGAAGA GTGATCAGTC AAAGGATGGC 
ATGATCCTCT TTAAAGGAAT CAAATCTGCT TCTGCTGAGA AAGCTCATCA GGAGAGTCTG 
CAGCATCAAA CTGATCTGGA CTCAGTATTT AAGATGCTGG AGGAAAACAT TATCACGTTT 
GTGAAGAACG AGCTGAAGAA GATGCAGAAT GATCTAAATC CAGATTATTC AGAGTATCAG 
AGGGATGAAG AGGAGGTGTT GGACAGTGAG GAAGAGAAGC AGAGAAGGAG CAGCAGAGAG 
GCATTTCTGA AACTTGCAGT TAACTTCCTG AGAATAATGA AACAGGAGCA GCTGGCTGAC 
TGTCTGCAGA GCAAAAATCC TGATCCACTT TGTGAGAACA CATTTAAATG TAACCTAAAG 
AAGAAGTTCC AGTGTGTCTC TGAGGGGATC GCTAAACCAG GAACCCGAAC CACTCTAAAT 
GAGATCTACA CAGAGCTCTG CATCACAGAG GGAGGGACCG GAGAGGTCAA CACTGAACAT 
GAGGTCAGAC AGATTGAAAT GTTTTCCTGG AAACCAAACA GACCAGAAAC ATCAATCAGG 
TGTGAAGACA TCTTTAAAGC TTCGACTGAA GGAGACAAAC CAATCAGAAC AGTTCTGACC 
AAAGGAGTGG CTGGCATCGG GAAAACACTC CTGACACAAA AGTTCACTTT GGACTGGGCT 
GAAGACAAGA CCAACCAGGA CATCCAGTTC ATTTTTCCAT TCACATTCAG AGAGCTGAAT 
TTACTGAAGG ATAAAAAGTT CAGCTTAGTG GGATTTATTC ATCACTTCTT CACTGAAATC 
AAAGAAGCAG GAATCTGTAA CTTTAAAAAG TTCAAAGTTG TGTTCATCTT TGATGGTCTG 
GATGAGTGTC GACTTCCTCT GAACTTCCAC ATCACTGAGA TCCTCACTGA TGTTACAGAG 
TCCACATCAG TGGATGTGCT GCTGACAAAC CTCATCAGGG GAAAGTTGCT TCCTTCTGCT 
CACATCTGGA TAACCACACG ACCTGCAGCA GCCAATCAGA TCCCTGCTGA CTATATAGAC 
ATGGTTACAG AGGTCAGAGG GTTCACTGAC CCAAAGAAGG AAGAGTACTT TAAGAAGAAA 
CTCAGAGACA AGGAAGAAGC CAACACCATA ATCTCTCACA TCAAGACATC ACGAAGCCTC 
CACATCATGT GTCACATCCC AATCTTCTGC TGGATCACTG CTACAGTTTT AGAGAGTTTG 
TTAAAAACCA AACAGGATGG AGGAAAACTG CCCAAGACAC TGACTGAGAT GTACATTCAC 
TTCCTGGTGG TTCAGACCAA ACTGAAGAAC ATCAAATATG ATGGAGGAGC TGAGACAGAT 
CCTCTCTGGA GTCCAGAGAA CAGAAAGATG ATTGAAGCAC TGGGGAAACT GGCTTTTGAG 
CAGCTGCTGA AAGGAAACCT GATCTTCTAT GAAACAGACC TGACAGAGTG TGGCATTAAT 
GTCAGAGAGG CCTCAGTGTA CTCAGGAGTG TTCACACAGA TTTTTAAAGA GGAGAGAGGA 
CTGTACCAAG AGAAGGTGTT CTGTTTCATC CATCTGAGCA TTCAGGAGTT TTTAGCTGCT 
CTTTATGTCC ATCTAACATT CACCAACTCT GGTATCAACC TGCTGAAAGA AGAAGAAACA 
GCCTCAGGAC AGACTGACGA GTCTTCAGTA AGACAGTTCT ACCAGAGTGC TGTGAACGAG 
GCTTTGAAGA GTTCAAATGG ACACTTGGAC TTGTTTCTTC GCTTCCTCCT TGGTCTTTCG 
ATGCCATCCA ATCAGGATCT CCTACAAGGC CTGCACACAC AAACAGGAAA CAGGTCACAG 
ATAAATCAGG AAGCAGTCAA GTACATTAAA GAAACAATGA ATGACACTCT CTCTTCAGAG 
AAAAGCATCA ATCTGCTCCA CTGTCTGAAT GAATTGAATG ATGACTCTAT AGTGAAGGAG 
GTCCAGCATC ACCTGAGTTC AGGACACCTG TCTAAAGTTA ATCTCTCTCC TGCTCAGTGG 
TCAGCTCTGG TCTTCATCTT ACTGTCATCA GAAACAGGTG TGGATGTGTT TGACTTGAGG 
AAATACTCAG CTTCAGAAGA GGCTCTTCTG CAGCTGCTGC CAGTGGTCAA AGTCTGTAAG 
AAAGCCCAGT TGAGTGGCTG TAACCTCACA GACAGAAGCT GTGAAGCTCT TTCCTCAATT 
CTAAGTTCTC AGTCCTCCAG ACTGAGAGAG CTGGACATGA GTAACAACAA CCTCCAGGAC 
TCAGGAGTGA AGCTGCTTTG TGTTGGACTT GGGAGTCCAC ACTGTATGCT GGAAACTCTC 
AGGCTGTCAG GCTGTCAGAT CACAGGAACA GGCTTCACCT CTCTGGCCAC AGCTCTGTGC 
TCCAACCCCT CCTATTTGAA AGAGCTGGAC TTGAGCTATA ATCACCCAGG AGACTCAGGA 
ATGAAGCTTT TGTCTGCTCT AAAGGAGGAT CCACATGTGA AACTCGACAT ACTCTGTGTG 
GAACCTCAAG GAGTCCAGTG GATGAGACCA GGTCTAAAGA AATATTTCTG TGATCTGACG 
CTGGATCCAA ACACAGCACA CAGAAACCTC AAACTGTCTA AAAACAACAA AGAGGTGACA 
CATGTGAAAG AAGACCAGCT GTATCCTGAT CATGATCATA GGTTTGATCA GTGGCCTCAG 
CTGCTGTGTA CCAACAGTCT GAGTGGTCGC TGTTACTGGG AGGTTGAGTG GAGTGGAGAG 
GTTCATGTTG CTGTAGTTTA TGGAGGAATC AGACGGAAAG GAGAAGAGGA TGAAAGCAGG 
TTTGGAAGGA CTGATCAATC CTGGAGTCTC AACTGCAGTG ATAATAAGGG TTACTATGCA 
GGTCACAAAT TTGATTTAAG AGCCATCCCT CTGCTCTCCT CAGCTCTTTC TCACAAAGTA 
GCAGTGTATG TGGACTGTTC TGCAGGAACT GTGTCTTTTT ACAGTGTCTC CTGTGATAAA 
CTGATCCACC TGCACACCTT CAACACCACC TTCACTGAAC CTGTATATGC TGGGTTTAGA 
GTGAAGCCTG ACTCTAGTAT CCACCTCTGT GCTGACTCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019203276.1
CDS	235..3459
Translation	MERPDSPEPETGRRSPQKDRPDFKIRKLTPDERITETPDALESEPGPSCVSLKSDQSKDGMILFKGIKSASAEKAHQESLQHQTDLDSVFKMLEENIITFVKNELKKMQNDLNPDYSEYQRDEEEVLDSEEEKQRRSSREAFLKLAVNFLRIMKQEQLADCLQSKNPDPLCENTFKCNLKKKFQCVSEGIAKPGTRTTLNEIYTELCITEGGTGEVNTEHEVRQIEMFSWKPNRPETSIRCEDIFKASTEGDKPIRTVLTKGVAGIGKTLLTQKFTLDWAEDKTNQDIQFIFPFTFRELNLLKDKKFSLVGFIHHFFTEIKEAGICNFKKFKVVFIFDGLDECRLPLNFHITEILTDVTESTSVDVLLTNLIRGKLLPSAHIWITTRPAAANQIPADYIDMVTEVRGFTDPKKEEYFKKKLRDKEEANTIISHIKTSRSLHIMCHIPIFCWITATVLESLLKTKQDGGKLPKTLTEMYIHFLVVQTKLKNIKYDGGAETDPLWSPENRKMIEALGKLAFEQLLKGNLIFYETDLTECGINVREASVYSGVFTQIFKEERGLYQEKVFCFIHLSIQEFLAALYVHLTFTNSGINLLKEEETASGQTDESSVRQFYQSAVNEALKSSNGHLDLFLRFLLGLSMPSNQDLLQGLHTQTGNRSQINQEAVKYIKETMNDTLSSEKSINLLHCLNELNDDSIVKEVQHHLSSGHLSKVNLSPAQWSALVFILLSSETGVDVFDLRKYSASEEALLQLLPVVKVCKKAQLSGCNLTDRSCEALSSILSSQSSRLRELDMSNNNLQDSGVKLLCVGLGSPHCMLETLRLSGCQITGTGFTSLATALCSNPSYLKELDLSYNHPGDSGMKLLSALKEDPHVKLDILCVEPQGVQWMRPGLKKYFCDLTLDPNTAHRNLKLSKNNKEVTHVKEDQLYPDHDHRFDQWPQLLCTNSLSGRCYWEVEWSGEVHVAVVYGGIRRKGEEDESRFGRTDQSWSLNCSDNKGYYAGHKFDLRAIPLLSSALSHKVAVYVDCSAGTVSFYSVSCDKLIHLHTFNTTFTEPVYAGFRVKPDSSIHLCADSE

Target ORF information:

RefSeq Version	XM_019347731.2
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus NLR family CARD domain-containing protein 3 (LOC102076273), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019347731.2

ORF Insert Sequence:

ATGGAAAGAC CAGACTCTCC TGAGCCTGAG ACCGGACGCA GATCTCCACA GAAAGACCGT 
CCTGACTTTA AAATCAGGAA ACTGACTCCT GATGAGAGGA TCACTGAGAC ACCAGATGCC 
CTTGAATCTG AACCTGGACC CAGCTGTGTG TCTCTGAAGA GTGATCAGTC AAAGGATGGC 
ATGATCCTCT TTAAAGGAAT CAAATCTGCT TCTGCTGAGA AAGCTCATCA GGAGAGTCTG 
CAGCATCAAA CTGATCTGGA CTCAGTATTT AAGATGCTGG AGGAAAACAT TATCACGTTT 
GTGAAGAACG AGCTGAAGAA GATGCAGAAT GATCTAAATC CAGATTATTC AGAGTATCAG 
AGGGATGAAG AGGAGGTGTT GGACAGTGAG GAAGAGAAGC AGAGAAGGAG CAGCAGAGAG 
GCATTTCTGA AACTTGCAGT TAACTTCCTG AGAATAATGA AACAGGAGCA GCTGGCTGAC 
TGTCTGCAGA GCAAAAATCC TGATCCACTT TGTGAGAACA CATTTAAATG TAACCTAAAG 
AAGAAGTTCC AGTGTGTCTC TGAGGGGATC GCTAAACCAG GAACCCGAAC CACTCTAAAT 
GAGATCTACA CAGAGCTCTG CATCACAGAG GGAGGGACCG GAGAGGTCAA CACTGAACAT 
GAGGTCAGAC AGATTGAAAT GTTTTCCTGG AAACCAAACA GACCAGAAAC ATCAATCAGG 
TGTGAAGACA TCTTTAAAGC TTCGACTGAA GGAGACAAAC CAATCAGAAC AGTTCTGACC 
AAAGGAGTGG CTGGCATCGG GAAAACACTC CTGACACAAA AGTTCACTTT GGACTGGGCT 
GAAGACAAGA CCAACCAGGA CATCCAGTTC ATTTTTCCAT TCACATTCAG AGAGCTGAAT 
TTACTGAAGG ATAAAAAGTT CAGCTTAGTG GGATTTATTC ATCACTTCTT CACTGAAATC 
AAAGAAGCAG GAATCTGTAA CTTTAAAAAG TTCAAAGTTG TGTTCATCTT TGATGGTCTG 
GATGAGTGTC GACTTCCTCT GAACTTCCAC ATCACTGAGA TCCTCACTGA TGTTACAGAG 
TCCACATCAG TGGATGTGCT GCTGACAAAC CTCATCAGGG GAAAGTTGCT TCCTTCTGCT 
CACATCTGGA TAACCACACG ACCTGCAGCA GCCAATCAGA TCCCTGCTGA CTATATAGAC 
ATGGTTACAG AGGTCAGAGG GTTCACTGAC CCAAAGAAGG AAGAGTACTT TAAGAAGAAA 
CTCAGAGACA AGGAAGAAGC CAACACCATA ATCTCTCACA TCAAGACATC ACGAAGCCTC 
CACATCATGT GTCACATCCC AATCTTCTGC TGGATCACTG CTACAGTTTT AGAGAGTTTG 
TTAAAAACCA AACAGGATGG AGGAAAACTG CCCAAGACAC TGACTGAGAT GTACATTCAC 
TTCCTGGTGG TTCAGACCAA ACTGAAGAAC ATCAAATATG ATGGAGGAGC TGAGACAGAT 
CCTCTCTGGA GTCCAGAGAA CAGAAAGATG ATTGAAGCAC TGGGGAAACT GGCTTTTGAG 
CAGCTGCTGA AAGGAAACCT GATCTTCTAT GAAACAGACC TGACAGAGTG TGGCATTAAT 
GTCAGAGAGG CCTCAGTGTA CTCAGGAGTG TTCACACAGA TTTTTAAAGA GGAGAGAGGA 
CTGTACCAAG AGAAGGTGTT CTGTTTCATC CATCTGAGCA TTCAGGAGTT TTTAGCTGCT 
CTTTATGTCC ATCTAACATT CACCAACTCT GGTATCAACC TGCTGAAAGA AGAAGAAACA 
GCCTCAGGAC AGACTGACGA GTCTTCAGTA AGACAGTTCT ACCAGAGTGC TGTGAACGAG 
GCTTTGAAGA GTTCAAATGG ACACTTGGAC TTGTTTCTTC GCTTCCTCCT TGGTCTTTCG 
ATGCCATCCA ATCAGGATCT CCTACAAGGC CTGCACACAC AAACAGGAAA CAGGTCACAG 
ATAAATCAGG AAGCAGTCAA GTACATTAAA GAAACAATGA ATGACACTCT CTCTTCAGAG 
AAAAGCATCA ATCTGCTCCA CTGTCTGAAT GAATTGAATG ATGACTCTAT AGTGAAGGAG 
GTCCAGCATC ACCTGAGTTC AGGACACCTG TCTAAAGTTA ATCTCTCTCC TGCTCAGTGG 
TCAGCTCTGG TCTTCATCTT ACTGTCATCA GAAACAGGTG TGGATGTGTT TGACTTGAGG 
AAATACTCAG CTTCAGAAGA GGCTCTTCTG CAGCTGCTGC CAGTGGTCAA AGTCTGTAAG 
AAAGCCCAGT TGAGTGGCTG TAACCTCACA GACAGAAGCT GTGAAGCTCT TTCCTCAATT 
CTAAGTTCTC AGTCCTCCAG ACTGAGAGAG CTGGACATGA GTAACAACAA CCTCCAGGAC 
TCAGGAGTGA AGCTGCTTTG TGTTGGACTT GGGAGTCCAC ACTGTATGCT GGAAACTCTC 
AGGCTGTCAG GCTGTCAGAT CACAGGAACA GGCTTCACCT CTCTGGCCAC AGCTCTGTGC 
TCCAACCCCT CCTATTTGAA AGAGCTGGAC TTGAGCTATA ATCACCCAGG AGACTCAGGA 
ATGAAGCTTT TGTCTGCTCT AAAGGAGGAT CCACATGTGA AACTCGACAT ACTCTGTGTG 
GAACCTCAAG GAGTCCAGTG GATGAGACCA GGTCTAAAGA AATATTTCTG TGATCTGACG 
CTGGATCCAA ACACAGCACA CAGAAACCTC AAACTGTCTA AAAACAACAA AGAGGTGACA 
CATGTGAAAG AAGACCAGCT GTATCCTGAT CATGATCATA GGTTTGATCA GTGGCCTCAG 
CTGCTGTGTA CCAACAGTCT GAGTGGTCGC TGTTACTGGG AGGTTGAGTG GAGTGGAGAG 
GTTCATGTTG CTGTAGTTTA TGGAGGAATC AGACGGAAAG GAGAAGAGGA TGAAAGCAGG 
TTTGGAAGGA CTGATCAATC CTGGAGTCTC AACTGCAGTG ATAATAAGGG TTACTATGCA 
GGTCACAAAT TTGATTTAAG AGCCATCCCT CTGCTCTCCT CAGCTCTTTC TCACAAAGTA 
GCAGTGTATG TGGACTGTTC TGCAGGAACT GTGTCTTTTT ACAGTGTCTC CTGTGATAAA 
CTGATCCACC TGCACACCTT CAACACCACC TTCACTGAAC CTGTATATGC TGGGTTTAGA 
GTGAAGCCTG ACTCTAGTAT CCACCTCTGT GCTGACTCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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LOC102076273 cDNA ORF clone, Oreochromis niloticus(Nile tilapia)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム