LOC100690562 cDNA ORF clone, Oreochromis niloticus(Nile tilapia)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100690562
Entrez Gene ID	100690562
Full Name	phosphorylase b kinase regulatory subunit alpha, liver isoform
Gene Type	protein-coding
Organism	Oreochromis niloticus(Nile tilapia)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019356820.1	XP_019212365.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
XM_019356826.1	XP_019212371.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X2
XM_019356829.1	XP_019212374.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X3
XM_019356832.1	XP_019212377.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X4
XM_019356826.2	XP_019212371.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X2
XM_019356829.2	XP_019212374.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X3
XM_019356820.2	XP_019212365.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
XM_019356832.2	XP_019212377.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X4

Pathways from BioSystems
Gene Ontology

The following LOC100690562 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100690562 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOr28622	XM_019356820.2 Latest version!	Oreochromis niloticus phosphorylase b kinase regulatory subunit alpha, liver isoform (LOC100690562), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOr28623	XM_019356826.2 Latest version!	Oreochromis niloticus phosphorylase b kinase regulatory subunit alpha, liver isoform (LOC100690562), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOr28624	XM_019356829.2 Latest version!	Oreochromis niloticus phosphorylase b kinase regulatory subunit alpha, liver isoform (LOC100690562), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOr28625	XM_019356832.2 Latest version!	Oreochromis niloticus phosphorylase b kinase regulatory subunit alpha, liver isoform (LOC100690562), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
OOr28623	XM_019356826.1	Oreochromis niloticus phosphorylase b kinase regulatory subunit alpha, liver isoform (LOC100690562), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOr28624	XM_019356829.1	Oreochromis niloticus phosphorylase b kinase regulatory subunit alpha, liver isoform (LOC100690562), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOr28622	XM_019356820.1	Oreochromis niloticus phosphorylase b kinase regulatory subunit alpha, liver isoform (LOC100690562), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOr28625	XM_019356832.1	Oreochromis niloticus phosphorylase b kinase regulatory subunit alpha, liver isoform (LOC100690562), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100690562 ( XM_019356820.1 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_019212365.1 Oreochromis niloticus phosphorylase b kinase regulatory subunit alpha, liver isoform (LOC100690562), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOr28622
Clone ID Related Accession (Same CDS sequence)	XM_019356820.1 , XM_019356820.2
Accession Version	XM_019356820.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031965.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GCAGTAACTC AGGAGTGCGG CTGGACGGCT ATGCCCGGCT GGTCCAGGAG 
ACTATCCTGT GCCATCAGAA CCCAGTGACA GGACTTCTTC CTGCCAGTGC GCAGAAGAAG 
GACGCCTGGG TGAGGGATAA TGTCTACAGT GTCCTGGCGG TGTGGGGGCT GGGTATGGCC 
TACCGGAAGA ATGCAGACCG CGATGAAGAC AAGGCCAAAG CTTACGAACT GGAGCAGAGT 
GTCGTGAAAC TGATGCAGGG GCTTCTGCAG TGCATGATGA GACAGGTGGC CAAGGTGGAG 
AAGTTCAAAC ACACCCAGAG TACAAAGGAT TGCTTGCATG CCAAATATGA TACTCCCACT 
TGTGCCACAG TGGTCAGGGA TGATCAGTGG GGTCATCTCC AGGTGGACGC CACCTCGATT 
TACCTGCTGA TGCTGGCACA GATGACAGCC TCAGGTCTTC GTATCATTTC CAACCTGGAC 
GAGGTAGCCT TCATCCAAAA CTTGGTCTTC TACATAGAGG CTGCCTACAA AGTAGCGGAC 
TATGGAATGT GGGAACGAGG TGACAAGACT AACCAGGGCA TTCCTGAGCT CAATGCCAGC 
TCTGTAGGAA TGGCTAAGGC AGCGCTTGAG GCTATAGATG AGCTGGATCT GTTTGGTGCT 
CACGGAGGGC CGAAGTCAGT CATCCATGTG TTGCCTGATG AAGTCGAACA CTGTCAGTCT 
ATTCTGTGCT CCATGCTGCC AAGAGCTTCA ACTTCAAAGG AAATAGATGC TGGTCTTCTG 
TCCATCACTT CTTTCCCTGC GTTTGCTGTG GAGGATGCTG ACCTGGTGGC TGTGACGAAG 
TCAGAGATCA TAAGCAAACT GCAGGGTCGC TATGGCTGCT GTCGCTTTAT CAGGGACGGA 
TATCGTTGTC CCAAAGAGGA TCCATCTCGG CTGCATTATG ATCCTGCTGA GCTGAAGCTA 
TTTGAGAATA TCGAGTGTGA GTGGCCTGTG TTTTGGACTT ACCTCATCCT GGATGGTATT 
TTTGCTGGTG ATCAAGTTCA GGTGCAAGAG TACCGTGAAG CACTGGAGGG TGTTTTAATC 
AGAGGGAAGA ATGGCATTAA GTTGGTGCCT GAACTTTATT CTGTTCCTCC TGACAAGGTT 
GAGGAGGAGT ATAGCAATCC TCACTCAGTA GACAGGGTAG CCATGGGCCA GCTGCCACAC 
ATGTGGGGAC AGTCACTCTA CATCTTGGGG TCCCTTGTGG CTGAGGGTTT TTTAGCACCA 
GGAGAGATAG ATCCGCTCAA CAGGAGATTC TCTACAAGCT TCAAACCAGA TGTGGTGGTA 
CAAGTTTGTG TTCTAGCAGA GTCGGAGGAG ATCCAGGAGT TGTTAAGAGA TCTGGGGATT 
GAGGTACAGA CGATGGCAGA AGTTCTGCCC ATCAGGGTCA TGCCAGCTCG CATCCTGAGC 
CATGTCTATG TTAGACTGGG GAACAGCAAG AAGCTGAATC TGAGTGGGCG GCCATACAGA 
CACATTGGTG TCCTGGGAAC ATCCAAATTC TACGAGATCC GAAATCGCTC TTACATATTC 
ACCCCTCAGT TTCTGGATCA GCACCATTTC TATCTGGCTC TGGACAACCA GATGATTGTG 
GAGATGTTAC GGACCGAGCT GGCCTATCTC TCTTGTTGCT GGAGGATGAC AGGACGTCCC 
ACGCTCACAT TCCCAATCAC CCGTAGCATG CTGGATGAGG ATGGAGAAAC AATTGATTCA 
TGTATCCTAT CAACTCTAAG GAAACTGCAG GATGGCTATT TTGCTGGAGC GAGGGTGCAG 
ATGTCAGACG TCTCCAGTGT CCAGACCACT TCGTTTCACA CTCGTCTCAG CTTCCTGGAT 
GAAGAGAATG ATGACATATT ACTTGAGGAT GAAGGCGATG ATGATGATGG ATATGGAGAG 
GAGTATAATA CCTGTGGTCC CTCGGAGGAC TCAAAAGATG TATTTGACCA GTACCTCACC 
CAGCTCCTTC ACAGCACCAC TACCAAGTGC CATCTTCCTC CCATCCAGAG GGGGCAGCAC 
CACGTCTTCA GTCCAGAACA TACCACAAGA GACATCCTGT CTTTTATGGC GCAGGTCCAG 
GGCCTGAACG TTCCCAAGTC TTCCATGTAT CTGCCTGTGA CTCCGGTCAA GAGCAAACAT 
CGCAAATCTC TCAACCTTCT TGATGTTCCT CATCCTCAGC ACGGCCCACA GGCGAAACAG 
CACAAGCCAC ACTCTGCTGC TGATCTTCAC CTGCCTCGAG ACTCTCAGGG CAACACAGAC 
TTTGCAGCGT TGGTCAGGCA GCTAAAAGAA TGCCCCACTC TTCAGGACCA GGCTGACATC 
CTCTACATTC TCTATGTGAT GAAAGGAGCT GATTGGCTGG TGGAGTTGTC AGGTCCTGGG 
CAGGGTGGGG TCAGCGTGCG ATCACTGTTG GAGGAGCTGT ACGTAGAAGC CGGAGCCTGC 
AAAGAGTGGG CGCTCATTAG GTACATCTCT GGAATACTGC GCAAGAGGGT GGAGGTCCTC 
GCTGAGGCCT GCACAGATCT GATTTCCCAT CACAAGCAGC TGACTGTAGG TCTACCTCCT 
CAACCCAGGG AAAGAGTGAT CACAGTCCCT CTTCCTCCGG ATGAGTTAAA CAGCCTCATC 
TACGAAGCCA GTGGTCAGGA CATAAGTGTA GCAGTGCTCA CTCAGGAAAT TATGGTGTAT 
CTAGCCATGT ATGTGCGCTC CCAGCCCGCT CTGTTCGGGG ACATGCTCAG ACTCAGGATA 
GGACTCATCA TGCAAGTGAT GGCCACTGAG TTAGCTCGTA GCCTGCACTG TTCTGGGGAG 
GAGGCGTCAG AGAGTTTGAT GAGCCTGAGT CCATTTGGCA TGAAGAACCT ACTGCATCAC 
ATCCTCAGTG GCAAAGAATT TGGGGTGGAG AGAAGCATGC GCCCAATCCA GTCTACAGCC 
ACTAGCCCTG CGATATCCAT CCATGAACTA GGTCACACGG GAGCTACAAA GACTGAACGC 
ACAGGAATAC ACAAGCTAAA GAGTGAGATA AAACAGCTGG ATGACTCTCG CCCTACAAGT 
ATCTTCAGTG GCGGTTTTTC CTTGAGTAGC AATGTCACAT CTCCTCGCTC TACGCGTTGC 
AGCAGCCCTT CCACTCCCAG TGGAATGCTG TCCCCGGTGA GCACTTGTCC AGGAGACGGT 
CAGCTGCACT GGGAGGAGAG GCAAGGTCAG TGGCTGAGGA GACGCAGGCT AGATGGTGCC 
ATCAACAGAG TACCTATGGG TTTCTACCAA AAGGTGTGGA AGATCCTGCA GAAGTGCCAC 
GGTCTGTCCA TCGACGGATA CGTGTTGCCC TCTTCTACCA CAAGAGAGAT GACAGCAGGA 
GAGATTAAGT TTGCAGTGCA GGTTGAATCT GTGCTGAACC ACGTCCCACA GCCAGAGTAC 
CGGCAGCTGC TAGTGGAGAC TGTGATGGTT GTGGGCCTGA TAGCGGACGT AGACGTGGAC 
AACATCGGCG GCATCATCCA CGTGGATCGC GTGTTGCATT TGGCCAATGA CCTTTTCCTC 
AGTGACCAGA AATCCCACGG CGCCAGTGAG TATTTCCTTG AGAAGGACCC AGCAACCGGA 
ATCTGCAACT TTTTCTACGA CAGCGCTCCT AGTGGTAGCT ATGGCACCAT GACCTATCTC 
TCCAAAGCGG CAGTCACTTA CGTCCAGGAC TTCCTGCCAA GTTCCAGCTG CCTGATGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019212365.1
CDS	333..4055
Translation	MRSRSNSGVRLDGYARLVQETILCHQNPVTGLLPASAQKKDAWVRDNVYSVLAVWGLGMAYRKNADRDEDKAKAYELEQSVVKLMQGLLQCMMRQVAKVEKFKHTQSTKDCLHAKYDTPTCATVVRDDQWGHLQVDATSIYLLMLAQMTASGLRIISNLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLFGAHGGPKSVIHVLPDEVEHCQSILCSMLPRASTSKEIDAGLLSITSFPAFAVEDADLVAVTKSEIISKLQGRYGCCRFIRDGYRCPKEDPSRLHYDPAELKLFENIECEWPVFWTYLILDGIFAGDQVQVQEYREALEGVLIRGKNGIKLVPELYSVPPDKVEEEYSNPHSVDRVAMGQLPHMWGQSLYILGSLVAEGFLAPGEIDPLNRRFSTSFKPDVVVQVCVLAESEEIQELLRDLGIEVQTMAEVLPIRVMPARILSHVYVRLGNSKKLNLSGRPYRHIGVLGTSKFYEIRNRSYIFTPQFLDQHHFYLALDNQMIVEMLRTELAYLSCCWRMTGRPTLTFPITRSMLDEDGETIDSCILSTLRKLQDGYFAGARVQMSDVSSVQTTSFHTRLSFLDEENDDILLEDEGDDDDGYGEEYNTCGPSEDSKDVFDQYLTQLLHSTTTKCHLPPIQRGQHHVFSPEHTTRDILSFMAQVQGLNVPKSSMYLPVTPVKSKHRKSLNLLDVPHPQHGPQAKQHKPHSAADLHLPRDSQGNTDFAALVRQLKECPTLQDQADILYILYVMKGADWLVELSGPGQGGVSVRSLLEELYVEAGACKEWALIRYISGILRKRVEVLAEACTDLISHHKQLTVGLPPQPRERVITVPLPPDELNSLIYEASGQDISVAVLTQEIMVYLAMYVRSQPALFGDMLRLRIGLIMQVMATELARSLHCSGEEASESLMSLSPFGMKNLLHHILSGKEFGVERSMRPIQSTATSPAISIHELGHTGATKTERTGIHKLKSEIKQLDDSRPTSIFSGGFSLSSNVTSPRSTRCSSPSTPSGMLSPVSTCPGDGQLHWEERQGQWLRRRRLDGAINRVPMGFYQKVWKILQKCHGLSIDGYVLPSSTTREMTAGEIKFAVQVESVLNHVPQPEYRQLLVETVMVVGLIADVDVDNIGGIIHVDRVLHLANDLFLSDQKSHGASEYFLEKDPATGICNFFYDSAPSGSYGTMTYLSKAAVTYVQDFLPSSSCLMQ

Target ORF information:

RefSeq Version	XM_019356820.1
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus phosphorylase b kinase regulatory subunit alpha, liver isoform (LOC100690562), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019356820.1

ORF Insert Sequence:

ATGAGGAGTC GCAGTAACTC AGGAGTGCGG CTGGACGGCT ATGCCCGGCT GGTCCAGGAG 
ACTATCCTGT GCCATCAGAA CCCAGTGACA GGACTTCTTC CTGCCAGTGC GCAGAAGAAG 
GACGCCTGGG TGAGGGATAA TGTCTACAGT GTCCTGGCGG TGTGGGGGCT GGGTATGGCC 
TACCGGAAGA ATGCAGACCG CGATGAAGAC AAGGCCAAAG CTTACGAACT GGAGCAGAGT 
GTCGTGAAAC TGATGCAGGG GCTTCTGCAG TGCATGATGA GACAGGTGGC CAAGGTGGAG 
AAGTTCAAAC ACACCCAGAG TACAAAGGAT TGCTTGCATG CCAAATATGA TACTCCCACT 
TGTGCCACAG TGGTCAGGGA TGATCAGTGG GGTCATCTCC AGGTGGACGC CACCTCGATT 
TACCTGCTGA TGCTGGCACA GATGACAGCC TCAGGTCTTC GTATCATTTC CAACCTGGAC 
GAGGTAGCCT TCATCCAAAA CTTGGTCTTC TACATAGAGG CTGCCTACAA AGTAGCGGAC 
TATGGAATGT GGGAACGAGG TGACAAGACT AACCAGGGCA TTCCTGAGCT CAATGCCAGC 
TCTGTAGGAA TGGCTAAGGC AGCGCTTGAG GCTATAGATG AGCTGGATCT GTTTGGTGCT 
CACGGAGGGC CGAAGTCAGT CATCCATGTG TTGCCTGATG AAGTCGAACA CTGTCAGTCT 
ATTCTGTGCT CCATGCTGCC AAGAGCTTCA ACTTCAAAGG AAATAGATGC TGGTCTTCTG 
TCCATCACTT CTTTCCCTGC GTTTGCTGTG GAGGATGCTG ACCTGGTGGC TGTGACGAAG 
TCAGAGATCA TAAGCAAACT GCAGGGTCGC TATGGCTGCT GTCGCTTTAT CAGGGACGGA 
TATCGTTGTC CCAAAGAGGA TCCATCTCGG CTGCATTATG ATCCTGCTGA GCTGAAGCTA 
TTTGAGAATA TCGAGTGTGA GTGGCCTGTG TTTTGGACTT ACCTCATCCT GGATGGTATT 
TTTGCTGGTG ATCAAGTTCA GGTGCAAGAG TACCGTGAAG CACTGGAGGG TGTTTTAATC 
AGAGGGAAGA ATGGCATTAA GTTGGTGCCT GAACTTTATT CTGTTCCTCC TGACAAGGTT 
GAGGAGGAGT ATAGCAATCC TCACTCAGTA GACAGGGTAG CCATGGGCCA GCTGCCACAC 
ATGTGGGGAC AGTCACTCTA CATCTTGGGG TCCCTTGTGG CTGAGGGTTT TTTAGCACCA 
GGAGAGATAG ATCCGCTCAA CAGGAGATTC TCTACAAGCT TCAAACCAGA TGTGGTGGTA 
CAAGTTTGTG TTCTAGCAGA GTCGGAGGAG ATCCAGGAGT TGTTAAGAGA TCTGGGGATT 
GAGGTACAGA CGATGGCAGA AGTTCTGCCC ATCAGGGTCA TGCCAGCTCG CATCCTGAGC 
CATGTCTATG TTAGACTGGG GAACAGCAAG AAGCTGAATC TGAGTGGGCG GCCATACAGA 
CACATTGGTG TCCTGGGAAC ATCCAAATTC TACGAGATCC GAAATCGCTC TTACATATTC 
ACCCCTCAGT TTCTGGATCA GCACCATTTC TATCTGGCTC TGGACAACCA GATGATTGTG 
GAGATGTTAC GGACCGAGCT GGCCTATCTC TCTTGTTGCT GGAGGATGAC AGGACGTCCC 
ACGCTCACAT TCCCAATCAC CCGTAGCATG CTGGATGAGG ATGGAGAAAC AATTGATTCA 
TGTATCCTAT CAACTCTAAG GAAACTGCAG GATGGCTATT TTGCTGGAGC GAGGGTGCAG 
ATGTCAGACG TCTCCAGTGT CCAGACCACT TCGTTTCACA CTCGTCTCAG CTTCCTGGAT 
GAAGAGAATG ATGACATATT ACTTGAGGAT GAAGGCGATG ATGATGATGG ATATGGAGAG 
GAGTATAATA CCTGTGGTCC CTCGGAGGAC TCAAAAGATG TATTTGACCA GTACCTCACC 
CAGCTCCTTC ACAGCACCAC TACCAAGTGC CATCTTCCTC CCATCCAGAG GGGGCAGCAC 
CACGTCTTCA GTCCAGAACA TACCACAAGA GACATCCTGT CTTTTATGGC GCAGGTCCAG 
GGCCTGAACG TTCCCAAGTC TTCCATGTAT CTGCCTGTGA CTCCGGTCAA GAGCAAACAT 
CGCAAATCTC TCAACCTTCT TGATGTTCCT CATCCTCAGC ACGGCCCACA GGCGAAACAG 
CACAAGCCAC ACTCTGCTGC TGATCTTCAC CTGCCTCGAG ACTCTCAGGG CAACACAGAC 
TTTGCAGCGT TGGTCAGGCA GCTAAAAGAA TGCCCCACTC TTCAGGACCA GGCTGACATC 
CTCTACATTC TCTATGTGAT GAAAGGAGCT GATTGGCTGG TGGAGTTGTC AGGTCCTGGG 
CAGGGTGGGG TCAGCGTGCG ATCACTGTTG GAGGAGCTGT ACGTAGAAGC CGGAGCCTGC 
AAAGAGTGGG CGCTCATTAG GTACATCTCT GGAATACTGC GCAAGAGGGT GGAGGTCCTC 
GCTGAGGCCT GCACAGATCT GATTTCCCAT CACAAGCAGC TGACTGTAGG TCTACCTCCT 
CAACCCAGGG AAAGAGTGAT CACAGTCCCT CTTCCTCCGG ATGAGTTAAA CAGCCTCATC 
TACGAAGCCA GTGGTCAGGA CATAAGTGTA GCAGTGCTCA CTCAGGAAAT TATGGTGTAT 
CTAGCCATGT ATGTGCGCTC CCAGCCCGCT CTGTTCGGGG ACATGCTCAG ACTCAGGATA 
GGACTCATCA TGCAAGTGAT GGCCACTGAG TTAGCTCGTA GCCTGCACTG TTCTGGGGAG 
GAGGCGTCAG AGAGTTTGAT GAGCCTGAGT CCATTTGGCA TGAAGAACCT ACTGCATCAC 
ATCCTCAGTG GCAAAGAATT TGGGGTGGAG AGAAGCATGC GCCCAATCCA GTCTACAGCC 
ACTAGCCCTG CGATATCCAT CCATGAACTA GGTCACACGG GAGCTACAAA GACTGAACGC 
ACAGGAATAC ACAAGCTAAA GAGTGAGATA AAACAGCTGG ATGACTCTCG CCCTACAAGT 
ATCTTCAGTG GCGGTTTTTC CTTGAGTAGC AATGTCACAT CTCCTCGCTC TACGCGTTGC 
AGCAGCCCTT CCACTCCCAG TGGAATGCTG TCCCCGGTGA GCACTTGTCC AGGAGACGGT 
CAGCTGCACT GGGAGGAGAG GCAAGGTCAG TGGCTGAGGA GACGCAGGCT AGATGGTGCC 
ATCAACAGAG TACCTATGGG TTTCTACCAA AAGGTGTGGA AGATCCTGCA GAAGTGCCAC 
GGTCTGTCCA TCGACGGATA CGTGTTGCCC TCTTCTACCA CAAGAGAGAT GACAGCAGGA 
GAGATTAAGT TTGCAGTGCA GGTTGAATCT GTGCTGAACC ACGTCCCACA GCCAGAGTAC 
CGGCAGCTGC TAGTGGAGAC TGTGATGGTT GTGGGCCTGA TAGCGGACGT AGACGTGGAC 
AACATCGGCG GCATCATCCA CGTGGATCGC GTGTTGCATT TGGCCAATGA CCTTTTCCTC 
AGTGACCAGA AATCCCACGG CGCCAGTGAG TATTTCCTTG AGAAGGACCC AGCAACCGGA 
ATCTGCAACT TTTTCTACGA CAGCGCTCCT AGTGGTAGCT ATGGCACCAT GACCTATCTC 
TCCAAAGCGG CAGTCACTTA CGTCCAGGAC TTCCTGCCAA GTTCCAGCTG CCTGATGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100690562 ( XM_019356820.2 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_019212365.1 Oreochromis niloticus phosphorylase b kinase regulatory subunit alpha, liver isoform (LOC100690562), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOr28622
Clone ID Related Accession (Same CDS sequence)	XM_019356820.1 , XM_019356820.2
Accession Version	XM_019356820.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031965.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_019356820.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GCAGTAACTC AGGAGTGCGG CTGGACGGCT ATGCCCGGCT GGTCCAGGAG 
ACTATCCTGT GCCATCAGAA CCCAGTGACA GGACTTCTTC CTGCCAGTGC GCAGAAGAAG 
GACGCCTGGG TGAGGGATAA TGTCTACAGT GTCCTGGCGG TGTGGGGGCT GGGTATGGCC 
TACCGGAAGA ATGCAGACCG CGATGAAGAC AAGGCCAAAG CTTACGAACT GGAGCAGAGT 
GTCGTGAAAC TGATGCAGGG GCTTCTGCAG TGCATGATGA GACAGGTGGC CAAGGTGGAG 
AAGTTCAAAC ACACCCAGAG TACAAAGGAT TGCTTGCATG CCAAATATGA TACTCCCACT 
TGTGCCACAG TGGTCAGGGA TGATCAGTGG GGTCATCTCC AGGTGGACGC CACCTCGATT 
TACCTGCTGA TGCTGGCACA GATGACAGCC TCAGGTCTTC GTATCATTTC CAACCTGGAC 
GAGGTAGCCT TCATCCAAAA CTTGGTCTTC TACATAGAGG CTGCCTACAA AGTAGCGGAC 
TATGGAATGT GGGAACGAGG TGACAAGACT AACCAGGGCA TTCCTGAGCT CAATGCCAGC 
TCTGTAGGAA TGGCTAAGGC AGCGCTTGAG GCTATAGATG AGCTGGATCT GTTTGGTGCT 
CACGGAGGGC CGAAGTCAGT CATCCATGTG TTGCCTGATG AAGTCGAACA CTGTCAGTCT 
ATTCTGTGCT CCATGCTGCC AAGAGCTTCA ACTTCAAAGG AAATAGATGC TGGTCTTCTG 
TCCATCACTT CTTTCCCTGC GTTTGCTGTG GAGGATGCTG ACCTGGTGGC TGTGACGAAG 
TCAGAGATCA TAAGCAAACT GCAGGGTCGC TATGGCTGCT GTCGCTTTAT CAGGGACGGA 
TATCGTTGTC CCAAAGAGGA TCCATCTCGG CTGCATTATG ATCCTGCTGA GCTGAAGCTA 
TTTGAGAATA TCGAGTGTGA GTGGCCTGTG TTTTGGACTT ACCTCATCCT GGATGGTATT 
TTTGCTGGTG ATCAAGTTCA GGTGCAAGAG TACCGTGAAG CACTGGAGGG TGTTTTAATC 
AGAGGGAAGA ATGGCATTAA GTTGGTGCCT GAACTTTATT CTGTTCCTCC TGACAAGGTT 
GAGGAGGAGT ATAGCAATCC TCACTCAGTA GACAGGGTAG CCATGGGCCA GCTGCCACAC 
ATGTGGGGAC AGTCACTCTA CATCTTGGGG TCCCTTGTGG CTGAGGGTTT TTTAGCACCA 
GGAGAGATAG ATCCGCTCAA CAGGAGATTC TCTACAAGCT TCAAACCAGA TGTGGTGGTA 
CAAGTTTGTG TTCTAGCAGA GTCGGAGGAG ATCCAGGAGT TGTTAAGAGA TCTGGGGATT 
GAGGTACAGA CGATGGCAGA AGTTCTGCCC ATCAGGGTCA TGCCAGCTCG CATCCTGAGC 
CATGTCTATG TTAGACTGGG GAACAGCAAG AAGCTGAATC TGAGTGGGCG GCCATACAGA 
CACATTGGTG TCCTGGGAAC ATCCAAATTC TACGAGATCC GAAATCGCTC TTACATATTC 
ACCCCTCAGT TTCTGGATCA GCACCATTTC TATCTGGCTC TGGACAACCA GATGATTGTG 
GAGATGTTAC GGACCGAGCT GGCCTATCTC TCTTGTTGCT GGAGGATGAC AGGACGTCCC 
ACGCTCACAT TCCCAATCAC CCGTAGCATG CTGGATGAGG ATGGAGAAAC AATTGATTCA 
TGTATCCTAT CAACTCTAAG GAAACTGCAG GATGGCTATT TTGCTGGAGC GAGGGTGCAG 
ATGTCAGACG TCTCCAGTGT CCAGACCACT TCGTTTCACA CTCGTCTCAG CTTCCTGGAT 
GAAGAGAATG ATGACATATT ACTTGAGGAT GAAGGCGATG ATGATGATGG ATATGGAGAG 
GAGTATAATA CCTGTGGTCC CTCGGAGGAC TCAAAAGATG TATTTGACCA GTACCTCACC 
CAGCTCCTTC ACAGCACCAC TACCAAGTGC CATCTTCCTC CCATCCAGAG GGGGCAGCAC 
CACGTCTTCA GTCCAGAACA TACCACAAGA GACATCCTGT CTTTTATGGC GCAGGTCCAG 
GGCCTGAACG TTCCCAAGTC TTCCATGTAT CTGCCTGTGA CTCCGGTCAA GAGCAAACAT 
CGCAAATCTC TCAACCTTCT TGATGTTCCT CATCCTCAGC ACGGCCCACA GGCGAAACAG 
CACAAGCCAC ACTCTGCTGC TGATCTTCAC CTGCCTCGAG ACTCTCAGGG CAACACAGAC 
TTTGCAGCGT TGGTCAGGCA GCTAAAAGAA TGCCCCACTC TTCAGGACCA GGCTGACATC 
CTCTACATTC TCTATGTGAT GAAAGGAGCT GATTGGCTGG TGGAGTTGTC AGGTCCTGGG 
CAGGGTGGGG TCAGCGTGCG ATCACTGTTG GAGGAGCTGT ACGTAGAAGC CGGAGCCTGC 
AAAGAGTGGG CGCTCATTAG GTACATCTCT GGAATACTGC GCAAGAGGGT GGAGGTCCTC 
GCTGAGGCCT GCACAGATCT GATTTCCCAT CACAAGCAGC TGACTGTAGG TCTACCTCCT 
CAACCCAGGG AAAGAGTGAT CACAGTCCCT CTTCCTCCGG ATGAGTTAAA CAGCCTCATC 
TACGAAGCCA GTGGTCAGGA CATAAGTGTA GCAGTGCTCA CTCAGGAAAT TATGGTGTAT 
CTAGCCATGT ATGTGCGCTC CCAGCCCGCT CTGTTCGGGG ACATGCTCAG ACTCAGGATA 
GGACTCATCA TGCAAGTGAT GGCCACTGAG TTAGCTCGTA GCCTGCACTG TTCTGGGGAG 
GAGGCGTCAG AGAGTTTGAT GAGCCTGAGT CCATTTGGCA TGAAGAACCT ACTGCATCAC 
ATCCTCAGTG GCAAAGAATT TGGGGTGGAG AGAAGCATGC GCCCAATCCA GTCTACAGCC 
ACTAGCCCTG CGATATCCAT CCATGAACTA GGTCACACGG GAGCTACAAA GACTGAACGC 
ACAGGAATAC ACAAGCTAAA GAGTGAGATA AAACAGCTGG ATGACTCTCG CCCTACAAGT 
ATCTTCAGTG GCGGTTTTTC CTTGAGTAGC AATGTCACAT CTCCTCGCTC TACGCGTTGC 
AGCAGCCCTT CCACTCCCAG TGGAATGCTG TCCCCGGTGA GCACTTGTCC AGGAGACGGT 
CAGCTGCACT GGGAGGAGAG GCAAGGTCAG TGGCTGAGGA GACGCAGGCT AGATGGTGCC 
ATCAACAGAG TACCTATGGG TTTCTACCAA AAGGTGTGGA AGATCCTGCA GAAGTGCCAC 
GGTCTGTCCA TCGACGGATA CGTGTTGCCC TCTTCTACCA CAAGAGAGAT GACAGCAGGA 
GAGATTAAGT TTGCAGTGCA GGTTGAATCT GTGCTGAACC ACGTCCCACA GCCAGAGTAC 
CGGCAGCTGC TAGTGGAGAC TGTGATGGTT GTGGGCCTGA TAGCGGACGT AGACGTGGAC 
AACATCGGCG GCATCATCCA CGTGGATCGC GTGTTGCATT TGGCCAATGA CCTTTTCCTC 
AGTGACCAGA AATCCCACGG CGCCAGTGAG TATTTCCTTG AGAAGGACCC AGCAACCGGA 
ATCTGCAACT TTTTCTACGA CAGCGCTCCT AGTGGTAGCT ATGGCACCAT GACCTATCTC 
TCCAAAGCGG CAGTCACTTA CGTCCAGGAC TTCCTGCCAA GTTCCAGCTG CCTGATGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019212365.1
CDS	309..4031
Translation	MRSRSNSGVRLDGYARLVQETILCHQNPVTGLLPASAQKKDAWVRDNVYSVLAVWGLGMAYRKNADRDEDKAKAYELEQSVVKLMQGLLQCMMRQVAKVEKFKHTQSTKDCLHAKYDTPTCATVVRDDQWGHLQVDATSIYLLMLAQMTASGLRIISNLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLFGAHGGPKSVIHVLPDEVEHCQSILCSMLPRASTSKEIDAGLLSITSFPAFAVEDADLVAVTKSEIISKLQGRYGCCRFIRDGYRCPKEDPSRLHYDPAELKLFENIECEWPVFWTYLILDGIFAGDQVQVQEYREALEGVLIRGKNGIKLVPELYSVPPDKVEEEYSNPHSVDRVAMGQLPHMWGQSLYILGSLVAEGFLAPGEIDPLNRRFSTSFKPDVVVQVCVLAESEEIQELLRDLGIEVQTMAEVLPIRVMPARILSHVYVRLGNSKKLNLSGRPYRHIGVLGTSKFYEIRNRSYIFTPQFLDQHHFYLALDNQMIVEMLRTELAYLSCCWRMTGRPTLTFPITRSMLDEDGETIDSCILSTLRKLQDGYFAGARVQMSDVSSVQTTSFHTRLSFLDEENDDILLEDEGDDDDGYGEEYNTCGPSEDSKDVFDQYLTQLLHSTTTKCHLPPIQRGQHHVFSPEHTTRDILSFMAQVQGLNVPKSSMYLPVTPVKSKHRKSLNLLDVPHPQHGPQAKQHKPHSAADLHLPRDSQGNTDFAALVRQLKECPTLQDQADILYILYVMKGADWLVELSGPGQGGVSVRSLLEELYVEAGACKEWALIRYISGILRKRVEVLAEACTDLISHHKQLTVGLPPQPRERVITVPLPPDELNSLIYEASGQDISVAVLTQEIMVYLAMYVRSQPALFGDMLRLRIGLIMQVMATELARSLHCSGEEASESLMSLSPFGMKNLLHHILSGKEFGVERSMRPIQSTATSPAISIHELGHTGATKTERTGIHKLKSEIKQLDDSRPTSIFSGGFSLSSNVTSPRSTRCSSPSTPSGMLSPVSTCPGDGQLHWEERQGQWLRRRRLDGAINRVPMGFYQKVWKILQKCHGLSIDGYVLPSSTTREMTAGEIKFAVQVESVLNHVPQPEYRQLLVETVMVVGLIADVDVDNIGGIIHVDRVLHLANDLFLSDQKSHGASEYFLEKDPATGICNFFYDSAPSGSYGTMTYLSKAAVTYVQDFLPSSSCLMQ

Target ORF information:

RefSeq Version	XM_019356820.2
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus phosphorylase b kinase regulatory subunit alpha, liver isoform (LOC100690562), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019356820.2

ORF Insert Sequence:

ATGAGGAGTC GCAGTAACTC AGGAGTGCGG CTGGACGGCT ATGCCCGGCT GGTCCAGGAG 
ACTATCCTGT GCCATCAGAA CCCAGTGACA GGACTTCTTC CTGCCAGTGC GCAGAAGAAG 
GACGCCTGGG TGAGGGATAA TGTCTACAGT GTCCTGGCGG TGTGGGGGCT GGGTATGGCC 
TACCGGAAGA ATGCAGACCG CGATGAAGAC AAGGCCAAAG CTTACGAACT GGAGCAGAGT 
GTCGTGAAAC TGATGCAGGG GCTTCTGCAG TGCATGATGA GACAGGTGGC CAAGGTGGAG 
AAGTTCAAAC ACACCCAGAG TACAAAGGAT TGCTTGCATG CCAAATATGA TACTCCCACT 
TGTGCCACAG TGGTCAGGGA TGATCAGTGG GGTCATCTCC AGGTGGACGC CACCTCGATT 
TACCTGCTGA TGCTGGCACA GATGACAGCC TCAGGTCTTC GTATCATTTC CAACCTGGAC 
GAGGTAGCCT TCATCCAAAA CTTGGTCTTC TACATAGAGG CTGCCTACAA AGTAGCGGAC 
TATGGAATGT GGGAACGAGG TGACAAGACT AACCAGGGCA TTCCTGAGCT CAATGCCAGC 
TCTGTAGGAA TGGCTAAGGC AGCGCTTGAG GCTATAGATG AGCTGGATCT GTTTGGTGCT 
CACGGAGGGC CGAAGTCAGT CATCCATGTG TTGCCTGATG AAGTCGAACA CTGTCAGTCT 
ATTCTGTGCT CCATGCTGCC AAGAGCTTCA ACTTCAAAGG AAATAGATGC TGGTCTTCTG 
TCCATCACTT CTTTCCCTGC GTTTGCTGTG GAGGATGCTG ACCTGGTGGC TGTGACGAAG 
TCAGAGATCA TAAGCAAACT GCAGGGTCGC TATGGCTGCT GTCGCTTTAT CAGGGACGGA 
TATCGTTGTC CCAAAGAGGA TCCATCTCGG CTGCATTATG ATCCTGCTGA GCTGAAGCTA 
TTTGAGAATA TCGAGTGTGA GTGGCCTGTG TTTTGGACTT ACCTCATCCT GGATGGTATT 
TTTGCTGGTG ATCAAGTTCA GGTGCAAGAG TACCGTGAAG CACTGGAGGG TGTTTTAATC 
AGAGGGAAGA ATGGCATTAA GTTGGTGCCT GAACTTTATT CTGTTCCTCC TGACAAGGTT 
GAGGAGGAGT ATAGCAATCC TCACTCAGTA GACAGGGTAG CCATGGGCCA GCTGCCACAC 
ATGTGGGGAC AGTCACTCTA CATCTTGGGG TCCCTTGTGG CTGAGGGTTT TTTAGCACCA 
GGAGAGATAG ATCCGCTCAA CAGGAGATTC TCTACAAGCT TCAAACCAGA TGTGGTGGTA 
CAAGTTTGTG TTCTAGCAGA GTCGGAGGAG ATCCAGGAGT TGTTAAGAGA TCTGGGGATT 
GAGGTACAGA CGATGGCAGA AGTTCTGCCC ATCAGGGTCA TGCCAGCTCG CATCCTGAGC 
CATGTCTATG TTAGACTGGG GAACAGCAAG AAGCTGAATC TGAGTGGGCG GCCATACAGA 
CACATTGGTG TCCTGGGAAC ATCCAAATTC TACGAGATCC GAAATCGCTC TTACATATTC 
ACCCCTCAGT TTCTGGATCA GCACCATTTC TATCTGGCTC TGGACAACCA GATGATTGTG 
GAGATGTTAC GGACCGAGCT GGCCTATCTC TCTTGTTGCT GGAGGATGAC AGGACGTCCC 
ACGCTCACAT TCCCAATCAC CCGTAGCATG CTGGATGAGG ATGGAGAAAC AATTGATTCA 
TGTATCCTAT CAACTCTAAG GAAACTGCAG GATGGCTATT TTGCTGGAGC GAGGGTGCAG 
ATGTCAGACG TCTCCAGTGT CCAGACCACT TCGTTTCACA CTCGTCTCAG CTTCCTGGAT 
GAAGAGAATG ATGACATATT ACTTGAGGAT GAAGGCGATG ATGATGATGG ATATGGAGAG 
GAGTATAATA CCTGTGGTCC CTCGGAGGAC TCAAAAGATG TATTTGACCA GTACCTCACC 
CAGCTCCTTC ACAGCACCAC TACCAAGTGC CATCTTCCTC CCATCCAGAG GGGGCAGCAC 
CACGTCTTCA GTCCAGAACA TACCACAAGA GACATCCTGT CTTTTATGGC GCAGGTCCAG 
GGCCTGAACG TTCCCAAGTC TTCCATGTAT CTGCCTGTGA CTCCGGTCAA GAGCAAACAT 
CGCAAATCTC TCAACCTTCT TGATGTTCCT CATCCTCAGC ACGGCCCACA GGCGAAACAG 
CACAAGCCAC ACTCTGCTGC TGATCTTCAC CTGCCTCGAG ACTCTCAGGG CAACACAGAC 
TTTGCAGCGT TGGTCAGGCA GCTAAAAGAA TGCCCCACTC TTCAGGACCA GGCTGACATC 
CTCTACATTC TCTATGTGAT GAAAGGAGCT GATTGGCTGG TGGAGTTGTC AGGTCCTGGG 
CAGGGTGGGG TCAGCGTGCG ATCACTGTTG GAGGAGCTGT ACGTAGAAGC CGGAGCCTGC 
AAAGAGTGGG CGCTCATTAG GTACATCTCT GGAATACTGC GCAAGAGGGT GGAGGTCCTC 
GCTGAGGCCT GCACAGATCT GATTTCCCAT CACAAGCAGC TGACTGTAGG TCTACCTCCT 
CAACCCAGGG AAAGAGTGAT CACAGTCCCT CTTCCTCCGG ATGAGTTAAA CAGCCTCATC 
TACGAAGCCA GTGGTCAGGA CATAAGTGTA GCAGTGCTCA CTCAGGAAAT TATGGTGTAT 
CTAGCCATGT ATGTGCGCTC CCAGCCCGCT CTGTTCGGGG ACATGCTCAG ACTCAGGATA 
GGACTCATCA TGCAAGTGAT GGCCACTGAG TTAGCTCGTA GCCTGCACTG TTCTGGGGAG 
GAGGCGTCAG AGAGTTTGAT GAGCCTGAGT CCATTTGGCA TGAAGAACCT ACTGCATCAC 
ATCCTCAGTG GCAAAGAATT TGGGGTGGAG AGAAGCATGC GCCCAATCCA GTCTACAGCC 
ACTAGCCCTG CGATATCCAT CCATGAACTA GGTCACACGG GAGCTACAAA GACTGAACGC 
ACAGGAATAC ACAAGCTAAA GAGTGAGATA AAACAGCTGG ATGACTCTCG CCCTACAAGT 
ATCTTCAGTG GCGGTTTTTC CTTGAGTAGC AATGTCACAT CTCCTCGCTC TACGCGTTGC 
AGCAGCCCTT CCACTCCCAG TGGAATGCTG TCCCCGGTGA GCACTTGTCC AGGAGACGGT 
CAGCTGCACT GGGAGGAGAG GCAAGGTCAG TGGCTGAGGA GACGCAGGCT AGATGGTGCC 
ATCAACAGAG TACCTATGGG TTTCTACCAA AAGGTGTGGA AGATCCTGCA GAAGTGCCAC 
GGTCTGTCCA TCGACGGATA CGTGTTGCCC TCTTCTACCA CAAGAGAGAT GACAGCAGGA 
GAGATTAAGT TTGCAGTGCA GGTTGAATCT GTGCTGAACC ACGTCCCACA GCCAGAGTAC 
CGGCAGCTGC TAGTGGAGAC TGTGATGGTT GTGGGCCTGA TAGCGGACGT AGACGTGGAC 
AACATCGGCG GCATCATCCA CGTGGATCGC GTGTTGCATT TGGCCAATGA CCTTTTCCTC 
AGTGACCAGA AATCCCACGG CGCCAGTGAG TATTTCCTTG AGAAGGACCC AGCAACCGGA 
ATCTGCAACT TTTTCTACGA CAGCGCTCCT AGTGGTAGCT ATGGCACCAT GACCTATCTC 
TCCAAAGCGG CAGTCACTTA CGTCCAGGAC TTCCTGCCAA GTTCCAGCTG CCTGATGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100690562 ( XM_019356826.1 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_019212371.1 Oreochromis niloticus phosphorylase b kinase regulatory subunit alpha, liver isoform (LOC100690562), transcript variant X2, mRNA.

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CloneID	OOr28623
Clone ID Related Accession (Same CDS sequence)	XM_019356826.1 , XM_019356826.2
Accession Version	XM_019356826.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031965.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GCAGTAACTC AGGAGTGCGG CTGGACGGCT ATGCCCGGCT GGTCCAGGAG 
ACTATCCTGT GCCATCAGAA CCCAGTGACA GGACTTCTTC CTGCCAGTGC GCAGAAGAAG 
GACGCCTGGG TGAGGGATAA TGTCTACAGT GTCCTGGCGG TGTGGGGGCT GGGTATGGCC 
TACCGGAAGA ATGCAGACCG CGATGAAGAC AAGGCCAAAG CTTACGAACT GGAGCAGAGT 
GTCGTGAAAC TGATGCAGGG GCTTCTGCAG TGCATGATGA GACAGGTGGC CAAGGTGGAG 
AAGTTCAAAC ACACCCAGAG TACAAAGGAT TGCTTGCATG CCAAATATGA TACTCCCACT 
TGTGCCACAG TGGTCAGGGA TGATCAGTGG GGTCATCTCC AGGTGGACGC CACCTCGATT 
TACCTGCTGA TGCTGGCACA GATGACAGCC TCAGGTCTTC GTATCATTTC CAACCTGGAC 
GAGGTAGCCT TCATCCAAAA CTTGGTCTTC TACATAGAGG CTGCCTACAA AGTAGCGGAC 
TATGGAATGT GGGAACGAGG TGACAAGACT AACCAGGGCA TTCCTGAGCT CAATGCCAGC 
TCTGTAGGAA TGGCTAAGGC AGCGCTTGAG GCTATAGATG AGCTGGATCT GTTTGGTGCT 
CACGGAGGGC CGAAGTCAGT CATCCATGTG TTGCCTGATG AAGTCGAACA CTGTCAGTCT 
ATTCTGTGCT CCATGCTGCC AAGAGCTTCA ACTTCAAAGG AAATAGATGC TGGTCTTCTG 
TCCATCACTT CTTTCCCTGC GTTTGCTGTG GAGGATGCTG ACCTGGTGGC TGTGACGAAG 
TCAGAGATCA TAAGCAAACT GCAGGGTCGC TATGGCTGCT GTCGCTTTAT CAGGGACGGA 
TATCGTTGTC CCAAAGAGGA TCCATCTCGG CTGCATTATG ATCCTGCTGA GCTGAAGCTA 
TTTGAGAATA TCGAGTGTGA GTGGCCTGTG TTTTGGACTT ACCTCATCCT GGATGGTATT 
TTTGCTGGTG ATCAAGTTCA GGTGCAAGAG TACCGTGAAG CACTGGAGGG TGTTTTAATC 
AGAGGGAAGA ATGGCATTAA GTTGGTGCCT GAACTTTATT CTGTTCCTCC TGACAAGGTT 
GAGGAGGAGT ATAGCAATCC TCACTCAGTA GACAGGGTAG CCATGGGCCA GCTGCCACAC 
ATGTGGGGAC AGTCACTCTA CATCTTGGGG TCCCTTGTGG CTGAGGGTTT TTTAGCACCA 
GGAGAGATAG ATCCGCTCAA CAGGAGATTC TCTACAAGCT TCAAACCAGA TGTGGTGGTA 
CAAGTTTGTG TTCTAGCAGA GTCGGAGGAG ATCCAGGAGT TGTTAAGAGA TCTGGGGATT 
GAGGTACAGA CGATGGCAGA AGTTCTGCCC ATCAGGGTCA TGCCAGCTCG CATCCTGAGC 
CATGTCTATG TTAGACTGGG GAACAGCAAG AAGCTGAATC TGAGTGGGCG GCCATACAGA 
CACATTGGTG TCCTGGGAAC ATCCAAATTC TACGAGATCC GAAATCGCTC TTACATATTC 
ACCCCTCAGT TTCTGGATCA GCACCATTTC TATCTGGCTC TGGACAACCA GATGATTGTG 
GAGATGTTAC GGACCGAGCT GGCCTATCTC TCTTGTTGCT GGAGGATGAC AGGACGTCCC 
ACGCTCACAT TCCCAATCAC CCGTAGCATG CTGGATGAGG ATGGAGAAAC AATTGATTCA 
TGTATCCTAT CAACTCTAAG GAAACTGCAG GATGGCTATT TTGCTGGAGC GAGGGTGCAG 
ATGTCAGACG TCTCCAGTGT CCAGACCACT TCGTTTCACA CTCGTCTCAG CTTCCTGGAT 
GAAGAGAATG ATGACATATT ACTTGAGGAT GAAGGCGATG ATGATGATGG ATATGGAGAG 
GAGTATAATA CCTGTGGTCC CTCGGAGGAC TCAAAAGATG TATTTGACCA GTACCTCACC 
CAGCTCCTTC ACAGCACCAC TACCAAGTGC CATCTTCCTC CCATCCAGAG GGGGCAGCAC 
CACGTCTTCA GTCCAGAACA TACCACAAGA GACATCCTGT CTTTTATGGC GCAGGTCCAG 
GGCCTGAACG TTCCCAAGTC TTCCATGTAT CTGCCTGTGA CTCCGGTCAA GAGCAAACAT 
CGCAAATCTC TCAACCTTCT TGATGTTCCT CATCCTCAGC ACGGCCCACA GGCGAAACAG 
CACAAGCCAC ACTCTGCTGC TGATCTTCAC CTGCCTCGAG ACTCTCAGGG CAACACAGAC 
TTTGCAGCGT TGGTCAGGCA GCTAAAAGAA TGCCCCACTC TTCAGGACCA GGCTGACATC 
CTCTACATTC TCTATGTGAT GAAAGGAGCT GATTGGCTGG TGGAGTTGTC AGGTCCTGGG 
CAGGGTGGGG TCAGCGTGCG ATCACTGTTG GAGGAGCTGT ACGTAGAAGC CGGAGCCTGC 
AAAGAGTGGG CGCTCATTAG GTACATCTCT GGAATACTGC GCAAGAGGGT GGAGGTCCTC 
GCTGAGGCCT GCACAGATCT GATTTCCCAT CACAAGCAGC TGACTGTAGG TCTACCTCCT 
CAACCCAGGG AAAGAGTGAT CACAGTCCCT CTTCCTCCGG ATGAGTTAAA CAGCCTCATC 
TACGAAGCCA GTGGTCAGGA CATAAGTGTA GCAGTGCTCA CTCAGGAAAT TATGGTGTAT 
CTAGCCATGT ATGTGCGCTC CCAGCCCGCT CTGTTCGGGG ACATGCTCAG ACTCAGGATA 
GGACTCATCA TGCAAGTGAT GGCCACTGAG TTAGCTCGTA GCCTGCACTG TTCTGGGGAG 
GAGGCGTCAG AGAGTTTGAT GAGCCTGAGT CCATTTGGCA TGAAGAACCT ACTGCATCAC 
ATCCTCAGTG GCAAAGAATT TGGGGTGGAG AGAAGCATGC GCCCAATCCA GTCTACAGCC 
ACTAGCCCTG CGATATCCAT CCATGAACTA GGTCACACGG GAGCTACAAA GACTGAACGC 
ACAGGAATAC ACAAGCTAAA GAGTGAGATA AAACAGATCT TCAGTGGCGG TTTTTCCTTG 
AGTAGCAATG TCACATCTCC TCGCTCTACG CGTTGCAGCA GCCCTTCCAC TCCCAGTGGA 
ATGCTGTCCC CGGTGAGCAC TTGTCCAGGA GACGGTCAGC TGCACTGGGA GGAGAGGCAA 
GGTCAGTGGC TGAGGAGACG CAGGCTAGAT GGTGCCATCA ACAGAGTACC TATGGGTTTC 
TACCAAAAGG TGTGGAAGAT CCTGCAGAAG TGCCACGGTC TGTCCATCGA CGGATACGTG 
TTGCCCTCTT CTACCACAAG AGAGATGACA GCAGGAGAGA TTAAGTTTGC AGTGCAGGTT 
GAATCTGTGC TGAACCACGT CCCACAGCCA GAGTACCGGC AGCTGCTAGT GGAGACTGTG 
ATGGTTGTGG GCCTGATAGC GGACGTAGAC GTGGACAACA TCGGCGGCAT CATCCACGTG 
GATCGCGTGT TGCATTTGGC CAATGACCTT TTCCTCAGTG ACCAGAAATC CCACGGCGCC 
AGTGAGTATT TCCTTGAGAA GGACCCAGCA ACCGGAATCT GCAACTTTTT CTACGACAGC 
GCTCCTAGTG GTAGCTATGG CACCATGACC TATCTCTCCA AAGCGGCAGT CACTTACGTC 
CAGGACTTCC TGCCAAGTTC CAGCTGCCTG ATGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019212371.1
CDS	333..4031
Translation	MRSRSNSGVRLDGYARLVQETILCHQNPVTGLLPASAQKKDAWVRDNVYSVLAVWGLGMAYRKNADRDEDKAKAYELEQSVVKLMQGLLQCMMRQVAKVEKFKHTQSTKDCLHAKYDTPTCATVVRDDQWGHLQVDATSIYLLMLAQMTASGLRIISNLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLFGAHGGPKSVIHVLPDEVEHCQSILCSMLPRASTSKEIDAGLLSITSFPAFAVEDADLVAVTKSEIISKLQGRYGCCRFIRDGYRCPKEDPSRLHYDPAELKLFENIECEWPVFWTYLILDGIFAGDQVQVQEYREALEGVLIRGKNGIKLVPELYSVPPDKVEEEYSNPHSVDRVAMGQLPHMWGQSLYILGSLVAEGFLAPGEIDPLNRRFSTSFKPDVVVQVCVLAESEEIQELLRDLGIEVQTMAEVLPIRVMPARILSHVYVRLGNSKKLNLSGRPYRHIGVLGTSKFYEIRNRSYIFTPQFLDQHHFYLALDNQMIVEMLRTELAYLSCCWRMTGRPTLTFPITRSMLDEDGETIDSCILSTLRKLQDGYFAGARVQMSDVSSVQTTSFHTRLSFLDEENDDILLEDEGDDDDGYGEEYNTCGPSEDSKDVFDQYLTQLLHSTTTKCHLPPIQRGQHHVFSPEHTTRDILSFMAQVQGLNVPKSSMYLPVTPVKSKHRKSLNLLDVPHPQHGPQAKQHKPHSAADLHLPRDSQGNTDFAALVRQLKECPTLQDQADILYILYVMKGADWLVELSGPGQGGVSVRSLLEELYVEAGACKEWALIRYISGILRKRVEVLAEACTDLISHHKQLTVGLPPQPRERVITVPLPPDELNSLIYEASGQDISVAVLTQEIMVYLAMYVRSQPALFGDMLRLRIGLIMQVMATELARSLHCSGEEASESLMSLSPFGMKNLLHHILSGKEFGVERSMRPIQSTATSPAISIHELGHTGATKTERTGIHKLKSEIKQIFSGGFSLSSNVTSPRSTRCSSPSTPSGMLSPVSTCPGDGQLHWEERQGQWLRRRRLDGAINRVPMGFYQKVWKILQKCHGLSIDGYVLPSSTTREMTAGEIKFAVQVESVLNHVPQPEYRQLLVETVMVVGLIADVDVDNIGGIIHVDRVLHLANDLFLSDQKSHGASEYFLEKDPATGICNFFYDSAPSGSYGTMTYLSKAAVTYVQDFLPSSSCLMQ

Target ORF information:

RefSeq Version	XM_019356826.1
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus phosphorylase b kinase regulatory subunit alpha, liver isoform (LOC100690562), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019356826.1

ORF Insert Sequence:

ATGAGGAGTC GCAGTAACTC AGGAGTGCGG CTGGACGGCT ATGCCCGGCT GGTCCAGGAG 
ACTATCCTGT GCCATCAGAA CCCAGTGACA GGACTTCTTC CTGCCAGTGC GCAGAAGAAG 
GACGCCTGGG TGAGGGATAA TGTCTACAGT GTCCTGGCGG TGTGGGGGCT GGGTATGGCC 
TACCGGAAGA ATGCAGACCG CGATGAAGAC AAGGCCAAAG CTTACGAACT GGAGCAGAGT 
GTCGTGAAAC TGATGCAGGG GCTTCTGCAG TGCATGATGA GACAGGTGGC CAAGGTGGAG 
AAGTTCAAAC ACACCCAGAG TACAAAGGAT TGCTTGCATG CCAAATATGA TACTCCCACT 
TGTGCCACAG TGGTCAGGGA TGATCAGTGG GGTCATCTCC AGGTGGACGC CACCTCGATT 
TACCTGCTGA TGCTGGCACA GATGACAGCC TCAGGTCTTC GTATCATTTC CAACCTGGAC 
GAGGTAGCCT TCATCCAAAA CTTGGTCTTC TACATAGAGG CTGCCTACAA AGTAGCGGAC 
TATGGAATGT GGGAACGAGG TGACAAGACT AACCAGGGCA TTCCTGAGCT CAATGCCAGC 
TCTGTAGGAA TGGCTAAGGC AGCGCTTGAG GCTATAGATG AGCTGGATCT GTTTGGTGCT 
CACGGAGGGC CGAAGTCAGT CATCCATGTG TTGCCTGATG AAGTCGAACA CTGTCAGTCT 
ATTCTGTGCT CCATGCTGCC AAGAGCTTCA ACTTCAAAGG AAATAGATGC TGGTCTTCTG 
TCCATCACTT CTTTCCCTGC GTTTGCTGTG GAGGATGCTG ACCTGGTGGC TGTGACGAAG 
TCAGAGATCA TAAGCAAACT GCAGGGTCGC TATGGCTGCT GTCGCTTTAT CAGGGACGGA 
TATCGTTGTC CCAAAGAGGA TCCATCTCGG CTGCATTATG ATCCTGCTGA GCTGAAGCTA 
TTTGAGAATA TCGAGTGTGA GTGGCCTGTG TTTTGGACTT ACCTCATCCT GGATGGTATT 
TTTGCTGGTG ATCAAGTTCA GGTGCAAGAG TACCGTGAAG CACTGGAGGG TGTTTTAATC 
AGAGGGAAGA ATGGCATTAA GTTGGTGCCT GAACTTTATT CTGTTCCTCC TGACAAGGTT 
GAGGAGGAGT ATAGCAATCC TCACTCAGTA GACAGGGTAG CCATGGGCCA GCTGCCACAC 
ATGTGGGGAC AGTCACTCTA CATCTTGGGG TCCCTTGTGG CTGAGGGTTT TTTAGCACCA 
GGAGAGATAG ATCCGCTCAA CAGGAGATTC TCTACAAGCT TCAAACCAGA TGTGGTGGTA 
CAAGTTTGTG TTCTAGCAGA GTCGGAGGAG ATCCAGGAGT TGTTAAGAGA TCTGGGGATT 
GAGGTACAGA CGATGGCAGA AGTTCTGCCC ATCAGGGTCA TGCCAGCTCG CATCCTGAGC 
CATGTCTATG TTAGACTGGG GAACAGCAAG AAGCTGAATC TGAGTGGGCG GCCATACAGA 
CACATTGGTG TCCTGGGAAC ATCCAAATTC TACGAGATCC GAAATCGCTC TTACATATTC 
ACCCCTCAGT TTCTGGATCA GCACCATTTC TATCTGGCTC TGGACAACCA GATGATTGTG 
GAGATGTTAC GGACCGAGCT GGCCTATCTC TCTTGTTGCT GGAGGATGAC AGGACGTCCC 
ACGCTCACAT TCCCAATCAC CCGTAGCATG CTGGATGAGG ATGGAGAAAC AATTGATTCA 
TGTATCCTAT CAACTCTAAG GAAACTGCAG GATGGCTATT TTGCTGGAGC GAGGGTGCAG 
ATGTCAGACG TCTCCAGTGT CCAGACCACT TCGTTTCACA CTCGTCTCAG CTTCCTGGAT 
GAAGAGAATG ATGACATATT ACTTGAGGAT GAAGGCGATG ATGATGATGG ATATGGAGAG 
GAGTATAATA CCTGTGGTCC CTCGGAGGAC TCAAAAGATG TATTTGACCA GTACCTCACC 
CAGCTCCTTC ACAGCACCAC TACCAAGTGC CATCTTCCTC CCATCCAGAG GGGGCAGCAC 
CACGTCTTCA GTCCAGAACA TACCACAAGA GACATCCTGT CTTTTATGGC GCAGGTCCAG 
GGCCTGAACG TTCCCAAGTC TTCCATGTAT CTGCCTGTGA CTCCGGTCAA GAGCAAACAT 
CGCAAATCTC TCAACCTTCT TGATGTTCCT CATCCTCAGC ACGGCCCACA GGCGAAACAG 
CACAAGCCAC ACTCTGCTGC TGATCTTCAC CTGCCTCGAG ACTCTCAGGG CAACACAGAC 
TTTGCAGCGT TGGTCAGGCA GCTAAAAGAA TGCCCCACTC TTCAGGACCA GGCTGACATC 
CTCTACATTC TCTATGTGAT GAAAGGAGCT GATTGGCTGG TGGAGTTGTC AGGTCCTGGG 
CAGGGTGGGG TCAGCGTGCG ATCACTGTTG GAGGAGCTGT ACGTAGAAGC CGGAGCCTGC 
AAAGAGTGGG CGCTCATTAG GTACATCTCT GGAATACTGC GCAAGAGGGT GGAGGTCCTC 
GCTGAGGCCT GCACAGATCT GATTTCCCAT CACAAGCAGC TGACTGTAGG TCTACCTCCT 
CAACCCAGGG AAAGAGTGAT CACAGTCCCT CTTCCTCCGG ATGAGTTAAA CAGCCTCATC 
TACGAAGCCA GTGGTCAGGA CATAAGTGTA GCAGTGCTCA CTCAGGAAAT TATGGTGTAT 
CTAGCCATGT ATGTGCGCTC CCAGCCCGCT CTGTTCGGGG ACATGCTCAG ACTCAGGATA 
GGACTCATCA TGCAAGTGAT GGCCACTGAG TTAGCTCGTA GCCTGCACTG TTCTGGGGAG 
GAGGCGTCAG AGAGTTTGAT GAGCCTGAGT CCATTTGGCA TGAAGAACCT ACTGCATCAC 
ATCCTCAGTG GCAAAGAATT TGGGGTGGAG AGAAGCATGC GCCCAATCCA GTCTACAGCC 
ACTAGCCCTG CGATATCCAT CCATGAACTA GGTCACACGG GAGCTACAAA GACTGAACGC 
ACAGGAATAC ACAAGCTAAA GAGTGAGATA AAACAGATCT TCAGTGGCGG TTTTTCCTTG 
AGTAGCAATG TCACATCTCC TCGCTCTACG CGTTGCAGCA GCCCTTCCAC TCCCAGTGGA 
ATGCTGTCCC CGGTGAGCAC TTGTCCAGGA GACGGTCAGC TGCACTGGGA GGAGAGGCAA 
GGTCAGTGGC TGAGGAGACG CAGGCTAGAT GGTGCCATCA ACAGAGTACC TATGGGTTTC 
TACCAAAAGG TGTGGAAGAT CCTGCAGAAG TGCCACGGTC TGTCCATCGA CGGATACGTG 
TTGCCCTCTT CTACCACAAG AGAGATGACA GCAGGAGAGA TTAAGTTTGC AGTGCAGGTT 
GAATCTGTGC TGAACCACGT CCCACAGCCA GAGTACCGGC AGCTGCTAGT GGAGACTGTG 
ATGGTTGTGG GCCTGATAGC GGACGTAGAC GTGGACAACA TCGGCGGCAT CATCCACGTG 
GATCGCGTGT TGCATTTGGC CAATGACCTT TTCCTCAGTG ACCAGAAATC CCACGGCGCC 
AGTGAGTATT TCCTTGAGAA GGACCCAGCA ACCGGAATCT GCAACTTTTT CTACGACAGC 
GCTCCTAGTG GTAGCTATGG CACCATGACC TATCTCTCCA AAGCGGCAGT CACTTACGTC 
CAGGACTTCC TGCCAAGTTC CAGCTGCCTG ATGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100690562 ( XM_019356826.2 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_019212371.1 Oreochromis niloticus phosphorylase b kinase regulatory subunit alpha, liver isoform (LOC100690562), transcript variant X2, mRNA.

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CloneID	OOr28623
Clone ID Related Accession (Same CDS sequence)	XM_019356826.1 , XM_019356826.2
Accession Version	XM_019356826.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031965.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_019356826.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GCAGTAACTC AGGAGTGCGG CTGGACGGCT ATGCCCGGCT GGTCCAGGAG 
ACTATCCTGT GCCATCAGAA CCCAGTGACA GGACTTCTTC CTGCCAGTGC GCAGAAGAAG 
GACGCCTGGG TGAGGGATAA TGTCTACAGT GTCCTGGCGG TGTGGGGGCT GGGTATGGCC 
TACCGGAAGA ATGCAGACCG CGATGAAGAC AAGGCCAAAG CTTACGAACT GGAGCAGAGT 
GTCGTGAAAC TGATGCAGGG GCTTCTGCAG TGCATGATGA GACAGGTGGC CAAGGTGGAG 
AAGTTCAAAC ACACCCAGAG TACAAAGGAT TGCTTGCATG CCAAATATGA TACTCCCACT 
TGTGCCACAG TGGTCAGGGA TGATCAGTGG GGTCATCTCC AGGTGGACGC CACCTCGATT 
TACCTGCTGA TGCTGGCACA GATGACAGCC TCAGGTCTTC GTATCATTTC CAACCTGGAC 
GAGGTAGCCT TCATCCAAAA CTTGGTCTTC TACATAGAGG CTGCCTACAA AGTAGCGGAC 
TATGGAATGT GGGAACGAGG TGACAAGACT AACCAGGGCA TTCCTGAGCT CAATGCCAGC 
TCTGTAGGAA TGGCTAAGGC AGCGCTTGAG GCTATAGATG AGCTGGATCT GTTTGGTGCT 
CACGGAGGGC CGAAGTCAGT CATCCATGTG TTGCCTGATG AAGTCGAACA CTGTCAGTCT 
ATTCTGTGCT CCATGCTGCC AAGAGCTTCA ACTTCAAAGG AAATAGATGC TGGTCTTCTG 
TCCATCACTT CTTTCCCTGC GTTTGCTGTG GAGGATGCTG ACCTGGTGGC TGTGACGAAG 
TCAGAGATCA TAAGCAAACT GCAGGGTCGC TATGGCTGCT GTCGCTTTAT CAGGGACGGA 
TATCGTTGTC CCAAAGAGGA TCCATCTCGG CTGCATTATG ATCCTGCTGA GCTGAAGCTA 
TTTGAGAATA TCGAGTGTGA GTGGCCTGTG TTTTGGACTT ACCTCATCCT GGATGGTATT 
TTTGCTGGTG ATCAAGTTCA GGTGCAAGAG TACCGTGAAG CACTGGAGGG TGTTTTAATC 
AGAGGGAAGA ATGGCATTAA GTTGGTGCCT GAACTTTATT CTGTTCCTCC TGACAAGGTT 
GAGGAGGAGT ATAGCAATCC TCACTCAGTA GACAGGGTAG CCATGGGCCA GCTGCCACAC 
ATGTGGGGAC AGTCACTCTA CATCTTGGGG TCCCTTGTGG CTGAGGGTTT TTTAGCACCA 
GGAGAGATAG ATCCGCTCAA CAGGAGATTC TCTACAAGCT TCAAACCAGA TGTGGTGGTA 
CAAGTTTGTG TTCTAGCAGA GTCGGAGGAG ATCCAGGAGT TGTTAAGAGA TCTGGGGATT 
GAGGTACAGA CGATGGCAGA AGTTCTGCCC ATCAGGGTCA TGCCAGCTCG CATCCTGAGC 
CATGTCTATG TTAGACTGGG GAACAGCAAG AAGCTGAATC TGAGTGGGCG GCCATACAGA 
CACATTGGTG TCCTGGGAAC ATCCAAATTC TACGAGATCC GAAATCGCTC TTACATATTC 
ACCCCTCAGT TTCTGGATCA GCACCATTTC TATCTGGCTC TGGACAACCA GATGATTGTG 
GAGATGTTAC GGACCGAGCT GGCCTATCTC TCTTGTTGCT GGAGGATGAC AGGACGTCCC 
ACGCTCACAT TCCCAATCAC CCGTAGCATG CTGGATGAGG ATGGAGAAAC AATTGATTCA 
TGTATCCTAT CAACTCTAAG GAAACTGCAG GATGGCTATT TTGCTGGAGC GAGGGTGCAG 
ATGTCAGACG TCTCCAGTGT CCAGACCACT TCGTTTCACA CTCGTCTCAG CTTCCTGGAT 
GAAGAGAATG ATGACATATT ACTTGAGGAT GAAGGCGATG ATGATGATGG ATATGGAGAG 
GAGTATAATA CCTGTGGTCC CTCGGAGGAC TCAAAAGATG TATTTGACCA GTACCTCACC 
CAGCTCCTTC ACAGCACCAC TACCAAGTGC CATCTTCCTC CCATCCAGAG GGGGCAGCAC 
CACGTCTTCA GTCCAGAACA TACCACAAGA GACATCCTGT CTTTTATGGC GCAGGTCCAG 
GGCCTGAACG TTCCCAAGTC TTCCATGTAT CTGCCTGTGA CTCCGGTCAA GAGCAAACAT 
CGCAAATCTC TCAACCTTCT TGATGTTCCT CATCCTCAGC ACGGCCCACA GGCGAAACAG 
CACAAGCCAC ACTCTGCTGC TGATCTTCAC CTGCCTCGAG ACTCTCAGGG CAACACAGAC 
TTTGCAGCGT TGGTCAGGCA GCTAAAAGAA TGCCCCACTC TTCAGGACCA GGCTGACATC 
CTCTACATTC TCTATGTGAT GAAAGGAGCT GATTGGCTGG TGGAGTTGTC AGGTCCTGGG 
CAGGGTGGGG TCAGCGTGCG ATCACTGTTG GAGGAGCTGT ACGTAGAAGC CGGAGCCTGC 
AAAGAGTGGG CGCTCATTAG GTACATCTCT GGAATACTGC GCAAGAGGGT GGAGGTCCTC 
GCTGAGGCCT GCACAGATCT GATTTCCCAT CACAAGCAGC TGACTGTAGG TCTACCTCCT 
CAACCCAGGG AAAGAGTGAT CACAGTCCCT CTTCCTCCGG ATGAGTTAAA CAGCCTCATC 
TACGAAGCCA GTGGTCAGGA CATAAGTGTA GCAGTGCTCA CTCAGGAAAT TATGGTGTAT 
CTAGCCATGT ATGTGCGCTC CCAGCCCGCT CTGTTCGGGG ACATGCTCAG ACTCAGGATA 
GGACTCATCA TGCAAGTGAT GGCCACTGAG TTAGCTCGTA GCCTGCACTG TTCTGGGGAG 
GAGGCGTCAG AGAGTTTGAT GAGCCTGAGT CCATTTGGCA TGAAGAACCT ACTGCATCAC 
ATCCTCAGTG GCAAAGAATT TGGGGTGGAG AGAAGCATGC GCCCAATCCA GTCTACAGCC 
ACTAGCCCTG CGATATCCAT CCATGAACTA GGTCACACGG GAGCTACAAA GACTGAACGC 
ACAGGAATAC ACAAGCTAAA GAGTGAGATA AAACAGATCT TCAGTGGCGG TTTTTCCTTG 
AGTAGCAATG TCACATCTCC TCGCTCTACG CGTTGCAGCA GCCCTTCCAC TCCCAGTGGA 
ATGCTGTCCC CGGTGAGCAC TTGTCCAGGA GACGGTCAGC TGCACTGGGA GGAGAGGCAA 
GGTCAGTGGC TGAGGAGACG CAGGCTAGAT GGTGCCATCA ACAGAGTACC TATGGGTTTC 
TACCAAAAGG TGTGGAAGAT CCTGCAGAAG TGCCACGGTC TGTCCATCGA CGGATACGTG 
TTGCCCTCTT CTACCACAAG AGAGATGACA GCAGGAGAGA TTAAGTTTGC AGTGCAGGTT 
GAATCTGTGC TGAACCACGT CCCACAGCCA GAGTACCGGC AGCTGCTAGT GGAGACTGTG 
ATGGTTGTGG GCCTGATAGC GGACGTAGAC GTGGACAACA TCGGCGGCAT CATCCACGTG 
GATCGCGTGT TGCATTTGGC CAATGACCTT TTCCTCAGTG ACCAGAAATC CCACGGCGCC 
AGTGAGTATT TCCTTGAGAA GGACCCAGCA ACCGGAATCT GCAACTTTTT CTACGACAGC 
GCTCCTAGTG GTAGCTATGG CACCATGACC TATCTCTCCA AAGCGGCAGT CACTTACGTC 
CAGGACTTCC TGCCAAGTTC CAGCTGCCTG ATGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019212371.1
CDS	309..4007
Translation	MRSRSNSGVRLDGYARLVQETILCHQNPVTGLLPASAQKKDAWVRDNVYSVLAVWGLGMAYRKNADRDEDKAKAYELEQSVVKLMQGLLQCMMRQVAKVEKFKHTQSTKDCLHAKYDTPTCATVVRDDQWGHLQVDATSIYLLMLAQMTASGLRIISNLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLFGAHGGPKSVIHVLPDEVEHCQSILCSMLPRASTSKEIDAGLLSITSFPAFAVEDADLVAVTKSEIISKLQGRYGCCRFIRDGYRCPKEDPSRLHYDPAELKLFENIECEWPVFWTYLILDGIFAGDQVQVQEYREALEGVLIRGKNGIKLVPELYSVPPDKVEEEYSNPHSVDRVAMGQLPHMWGQSLYILGSLVAEGFLAPGEIDPLNRRFSTSFKPDVVVQVCVLAESEEIQELLRDLGIEVQTMAEVLPIRVMPARILSHVYVRLGNSKKLNLSGRPYRHIGVLGTSKFYEIRNRSYIFTPQFLDQHHFYLALDNQMIVEMLRTELAYLSCCWRMTGRPTLTFPITRSMLDEDGETIDSCILSTLRKLQDGYFAGARVQMSDVSSVQTTSFHTRLSFLDEENDDILLEDEGDDDDGYGEEYNTCGPSEDSKDVFDQYLTQLLHSTTTKCHLPPIQRGQHHVFSPEHTTRDILSFMAQVQGLNVPKSSMYLPVTPVKSKHRKSLNLLDVPHPQHGPQAKQHKPHSAADLHLPRDSQGNTDFAALVRQLKECPTLQDQADILYILYVMKGADWLVELSGPGQGGVSVRSLLEELYVEAGACKEWALIRYISGILRKRVEVLAEACTDLISHHKQLTVGLPPQPRERVITVPLPPDELNSLIYEASGQDISVAVLTQEIMVYLAMYVRSQPALFGDMLRLRIGLIMQVMATELARSLHCSGEEASESLMSLSPFGMKNLLHHILSGKEFGVERSMRPIQSTATSPAISIHELGHTGATKTERTGIHKLKSEIKQIFSGGFSLSSNVTSPRSTRCSSPSTPSGMLSPVSTCPGDGQLHWEERQGQWLRRRRLDGAINRVPMGFYQKVWKILQKCHGLSIDGYVLPSSTTREMTAGEIKFAVQVESVLNHVPQPEYRQLLVETVMVVGLIADVDVDNIGGIIHVDRVLHLANDLFLSDQKSHGASEYFLEKDPATGICNFFYDSAPSGSYGTMTYLSKAAVTYVQDFLPSSSCLMQ

Target ORF information:

RefSeq Version	XM_019356826.2
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus phosphorylase b kinase regulatory subunit alpha, liver isoform (LOC100690562), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019356826.2

ORF Insert Sequence:

ATGAGGAGTC GCAGTAACTC AGGAGTGCGG CTGGACGGCT ATGCCCGGCT GGTCCAGGAG 
ACTATCCTGT GCCATCAGAA CCCAGTGACA GGACTTCTTC CTGCCAGTGC GCAGAAGAAG 
GACGCCTGGG TGAGGGATAA TGTCTACAGT GTCCTGGCGG TGTGGGGGCT GGGTATGGCC 
TACCGGAAGA ATGCAGACCG CGATGAAGAC AAGGCCAAAG CTTACGAACT GGAGCAGAGT 
GTCGTGAAAC TGATGCAGGG GCTTCTGCAG TGCATGATGA GACAGGTGGC CAAGGTGGAG 
AAGTTCAAAC ACACCCAGAG TACAAAGGAT TGCTTGCATG CCAAATATGA TACTCCCACT 
TGTGCCACAG TGGTCAGGGA TGATCAGTGG GGTCATCTCC AGGTGGACGC CACCTCGATT 
TACCTGCTGA TGCTGGCACA GATGACAGCC TCAGGTCTTC GTATCATTTC CAACCTGGAC 
GAGGTAGCCT TCATCCAAAA CTTGGTCTTC TACATAGAGG CTGCCTACAA AGTAGCGGAC 
TATGGAATGT GGGAACGAGG TGACAAGACT AACCAGGGCA TTCCTGAGCT CAATGCCAGC 
TCTGTAGGAA TGGCTAAGGC AGCGCTTGAG GCTATAGATG AGCTGGATCT GTTTGGTGCT 
CACGGAGGGC CGAAGTCAGT CATCCATGTG TTGCCTGATG AAGTCGAACA CTGTCAGTCT 
ATTCTGTGCT CCATGCTGCC AAGAGCTTCA ACTTCAAAGG AAATAGATGC TGGTCTTCTG 
TCCATCACTT CTTTCCCTGC GTTTGCTGTG GAGGATGCTG ACCTGGTGGC TGTGACGAAG 
TCAGAGATCA TAAGCAAACT GCAGGGTCGC TATGGCTGCT GTCGCTTTAT CAGGGACGGA 
TATCGTTGTC CCAAAGAGGA TCCATCTCGG CTGCATTATG ATCCTGCTGA GCTGAAGCTA 
TTTGAGAATA TCGAGTGTGA GTGGCCTGTG TTTTGGACTT ACCTCATCCT GGATGGTATT 
TTTGCTGGTG ATCAAGTTCA GGTGCAAGAG TACCGTGAAG CACTGGAGGG TGTTTTAATC 
AGAGGGAAGA ATGGCATTAA GTTGGTGCCT GAACTTTATT CTGTTCCTCC TGACAAGGTT 
GAGGAGGAGT ATAGCAATCC TCACTCAGTA GACAGGGTAG CCATGGGCCA GCTGCCACAC 
ATGTGGGGAC AGTCACTCTA CATCTTGGGG TCCCTTGTGG CTGAGGGTTT TTTAGCACCA 
GGAGAGATAG ATCCGCTCAA CAGGAGATTC TCTACAAGCT TCAAACCAGA TGTGGTGGTA 
CAAGTTTGTG TTCTAGCAGA GTCGGAGGAG ATCCAGGAGT TGTTAAGAGA TCTGGGGATT 
GAGGTACAGA CGATGGCAGA AGTTCTGCCC ATCAGGGTCA TGCCAGCTCG CATCCTGAGC 
CATGTCTATG TTAGACTGGG GAACAGCAAG AAGCTGAATC TGAGTGGGCG GCCATACAGA 
CACATTGGTG TCCTGGGAAC ATCCAAATTC TACGAGATCC GAAATCGCTC TTACATATTC 
ACCCCTCAGT TTCTGGATCA GCACCATTTC TATCTGGCTC TGGACAACCA GATGATTGTG 
GAGATGTTAC GGACCGAGCT GGCCTATCTC TCTTGTTGCT GGAGGATGAC AGGACGTCCC 
ACGCTCACAT TCCCAATCAC CCGTAGCATG CTGGATGAGG ATGGAGAAAC AATTGATTCA 
TGTATCCTAT CAACTCTAAG GAAACTGCAG GATGGCTATT TTGCTGGAGC GAGGGTGCAG 
ATGTCAGACG TCTCCAGTGT CCAGACCACT TCGTTTCACA CTCGTCTCAG CTTCCTGGAT 
GAAGAGAATG ATGACATATT ACTTGAGGAT GAAGGCGATG ATGATGATGG ATATGGAGAG 
GAGTATAATA CCTGTGGTCC CTCGGAGGAC TCAAAAGATG TATTTGACCA GTACCTCACC 
CAGCTCCTTC ACAGCACCAC TACCAAGTGC CATCTTCCTC CCATCCAGAG GGGGCAGCAC 
CACGTCTTCA GTCCAGAACA TACCACAAGA GACATCCTGT CTTTTATGGC GCAGGTCCAG 
GGCCTGAACG TTCCCAAGTC TTCCATGTAT CTGCCTGTGA CTCCGGTCAA GAGCAAACAT 
CGCAAATCTC TCAACCTTCT TGATGTTCCT CATCCTCAGC ACGGCCCACA GGCGAAACAG 
CACAAGCCAC ACTCTGCTGC TGATCTTCAC CTGCCTCGAG ACTCTCAGGG CAACACAGAC 
TTTGCAGCGT TGGTCAGGCA GCTAAAAGAA TGCCCCACTC TTCAGGACCA GGCTGACATC 
CTCTACATTC TCTATGTGAT GAAAGGAGCT GATTGGCTGG TGGAGTTGTC AGGTCCTGGG 
CAGGGTGGGG TCAGCGTGCG ATCACTGTTG GAGGAGCTGT ACGTAGAAGC CGGAGCCTGC 
AAAGAGTGGG CGCTCATTAG GTACATCTCT GGAATACTGC GCAAGAGGGT GGAGGTCCTC 
GCTGAGGCCT GCACAGATCT GATTTCCCAT CACAAGCAGC TGACTGTAGG TCTACCTCCT 
CAACCCAGGG AAAGAGTGAT CACAGTCCCT CTTCCTCCGG ATGAGTTAAA CAGCCTCATC 
TACGAAGCCA GTGGTCAGGA CATAAGTGTA GCAGTGCTCA CTCAGGAAAT TATGGTGTAT 
CTAGCCATGT ATGTGCGCTC CCAGCCCGCT CTGTTCGGGG ACATGCTCAG ACTCAGGATA 
GGACTCATCA TGCAAGTGAT GGCCACTGAG TTAGCTCGTA GCCTGCACTG TTCTGGGGAG 
GAGGCGTCAG AGAGTTTGAT GAGCCTGAGT CCATTTGGCA TGAAGAACCT ACTGCATCAC 
ATCCTCAGTG GCAAAGAATT TGGGGTGGAG AGAAGCATGC GCCCAATCCA GTCTACAGCC 
ACTAGCCCTG CGATATCCAT CCATGAACTA GGTCACACGG GAGCTACAAA GACTGAACGC 
ACAGGAATAC ACAAGCTAAA GAGTGAGATA AAACAGATCT TCAGTGGCGG TTTTTCCTTG 
AGTAGCAATG TCACATCTCC TCGCTCTACG CGTTGCAGCA GCCCTTCCAC TCCCAGTGGA 
ATGCTGTCCC CGGTGAGCAC TTGTCCAGGA GACGGTCAGC TGCACTGGGA GGAGAGGCAA 
GGTCAGTGGC TGAGGAGACG CAGGCTAGAT GGTGCCATCA ACAGAGTACC TATGGGTTTC 
TACCAAAAGG TGTGGAAGAT CCTGCAGAAG TGCCACGGTC TGTCCATCGA CGGATACGTG 
TTGCCCTCTT CTACCACAAG AGAGATGACA GCAGGAGAGA TTAAGTTTGC AGTGCAGGTT 
GAATCTGTGC TGAACCACGT CCCACAGCCA GAGTACCGGC AGCTGCTAGT GGAGACTGTG 
ATGGTTGTGG GCCTGATAGC GGACGTAGAC GTGGACAACA TCGGCGGCAT CATCCACGTG 
GATCGCGTGT TGCATTTGGC CAATGACCTT TTCCTCAGTG ACCAGAAATC CCACGGCGCC 
AGTGAGTATT TCCTTGAGAA GGACCCAGCA ACCGGAATCT GCAACTTTTT CTACGACAGC 
GCTCCTAGTG GTAGCTATGG CACCATGACC TATCTCTCCA AAGCGGCAGT CACTTACGTC 
CAGGACTTCC TGCCAAGTTC CAGCTGCCTG ATGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100690562 ( XM_019356829.1 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_019212374.1 Oreochromis niloticus phosphorylase b kinase regulatory subunit alpha, liver isoform (LOC100690562), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOr28624
Clone ID Related Accession (Same CDS sequence)	XM_019356829.1 , XM_019356829.2
Accession Version	XM_019356829.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031965.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GCAGTAACTC AGGAGTGCGG CTGGACGGCT ATGCCCGGCT GGTCCAGGAG 
ACTATCCTGT GCCATCAGAA CCCAGTGACA GGACTTCTTC CTGCCAGTGC GCAGAAGAAG 
GACGCCTGGG TGAGGGATAA TGTCTACAGT GTCCTGGCGG TGTGGGGGCT GGGTATGGCC 
TACCGGAAGA ATGCAGACCG CGATGAAGAC AAGGCCAAAG CTTACGAACT GGAGCAGAGT 
GTCGTGAAAC TGATGCAGGG GCTTCTGCAG TGCATGATGA GACAGGTGGC CAAGGTGGAG 
AAGTTCAAAC ACACCCAGAG TACAAAGGAT TGCTTGCATG CCAAATATGA TACTCCCACT 
TGTGCCACAG TGGTCAGGGA TGATCAGTGG GGTCATCTCC AGGTGGACGC CACCTCGATT 
TACCTGCTGA TGCTGGCACA GATGACAGCC TCAGGTCTTC GTATCATTTC CAACCTGGAC 
GAGGTAGCCT TCATCCAAAA CTTGGTCTTC TACATAGAGG CTGCCTACAA AGTAGCGGAC 
TATGGAATGT GGGAACGAGG TGACAAGACT AACCAGGGCA TTCCTGAGCT CAATGCCAGC 
TCTGTAGGAA TGGCTAAGGC AGCGCTTGAG GCTATAGATG AGCTGGATCT GTTTGGTGCT 
CACGGAGGGC CGAAGTCAGT CATCCATGTG TTGCCTGATG AAGTCGAACA CTGTCAGTCT 
ATTCTGTGCT CCATGCTGCC AAGAGCTTCA ACTTCAAAGG AAATAGATGC TGGTCTTCTG 
TCCATCACTT CTTTCCCTGC GTTTGCTGTG GAGGATGCTG ACCTGGTGGC TGTGACGAAG 
TCAGAGATCA TAAGCAAACT GCAGGGTCGC TATGGCTGCT GTCGCTTTAT CAGGGACGGA 
TATCGTTGTC CCAAAGAGGA TCCATCTCGG CTGCATTATG ATCCTGCTGA GCTGAAGCTA 
TTTGAGAATA TCGAGTGTGA GTGGCCTGTG TTTTGGACTT ACCTCATCCT GGATGGTATT 
TTTGCTGGTG ATCAAGTTCA GGTGCAAGAG TACCGTGAAG CACTGGAGGG TGTTTTAATC 
AGAGGGAAGA ATGGCATTAA GTTGGTGCCT GAACTTTATT CTGTTCCTCC TGACAAGGTT 
GAGGAGGAGT ATAGCAATCC TCACTCAGTA GACAGGGTAG CCATGGGCCA GCTGCCACAC 
ATGTGGGGAC AGTCACTCTA CATCTTGGGG TCCCTTGTGG CTGAGGGTTT TTTAGCACCA 
GGAGAGATAG ATCCGCTCAA CAGGAGATTC TCTACAAGCT TCAAACCAGA TGTGGTGGTA 
CAAGTTTGTG TTCTAGCAGA GTCGGAGGAG ATCCAGGAGT TGTTAAGAGA TCTGGGGATT 
GAGGTACAGA CGATGGCAGA AGTTCTGCCC ATCAGGGTCA TGCCAGCTCG CATCCTGAGC 
CATGTCTATG TTAGACTGGG GAACAGCAAG AAGCTGAATC TGAGTGGGCG GCCATACAGA 
CACATTGGTG TCCTGGGAAC ATCCAAATTC TACGAGATCC GAAATCGCTC TTACATATTC 
ACCCCTCAGT TTCTGGATCA GCACCATTTC TATCTGGCTC TGGACAACCA GATGATTGTG 
GAGATGTTAC GGACCGAGCT GGCCTATCTC TCTTGTTGCT GGAGGATGAC AGGACGTCCC 
ACGCTCACAT TCCCAATCAC CCGTAGCATG CTGGATGAGG ATGGAGAAAC AATTGATTCA 
TGTATCCTAT CAACTCTAAG GAAACTGCAG GATGGCTATT TTGCTGGAGC GAGGGTGCAG 
ATGTCAGACG TCTCCAGTGT CCAGACCACT TCGTTTCACA CTCGTCTCAG CTTCCTGGAT 
GAAGAGAATG ATGACATATT ACTTGAGGAT GAAGGCGATG ATGATGATGG ATATGGAGAG 
GAGTATAATA CCTGTGGTCC CTCGGAGGAC TCAAAAGATG TATTTGACCA GTACCTCACC 
CAGCTCCTTC ACAGCACCAC TACCAAGTGC CATCTTCCTC CCATCCAGAG GGGGCAGCAC 
CACGTCTTCA GTCCAGAACA TACCACAAGA GACATCCTGT CTTTTATGGC GCAGGTCCAG 
GGCCTGAACG TTCCCAAGTC TTCCATGTAT CTGCCTGTGA CTCCGGTCAA GAGCAAACAT 
CGCAAATCTC TCAACCTTCT TGATGTTCCT CATCCTCAGC ACGGCCCACA GGCGAAACAG 
CACAAGCCAC ACTCTGCTGC TGATCTTCAC CTGCCTCGAG ACTCTCAGGG CAACACAGAC 
TTTGCAGCGT TGGTCAGGCA GCTAAAAGAA TGCCCCACTC TTCAGGACCA GGCTGACATC 
CTCTACATTC TCTATGTGAT GAAAGGAGCT GATTGGCTGG TGGAGTTGTC AGGTCCTGGG 
CAGGGTGGGG TCAGCGTGCG ATCACTGTTG GAGGAGCTGT ACGTAGAAGC CGGAGCCTGC 
AAAGAGTGGG CGCTCATTAG GTACATCTCT GGAATACTGC GCAAGAGGGT GGAGGTCCTC 
GCTGAGGCCT GCACAGATCT GATTTCCCAT CACAAGCAGC TGACTGTAGG TCTACCTCCT 
CAACCCAGGG AAAGAGTGAT CACAGTCCCT CTTCCTCCGG ATGAGTTAAA CAGCCTCATC 
TACGAAGCCA GTGGTCAGGA CATAAGTGTA GCAGTGCTCA CTCAGGAAAT TATGGTGTAT 
CTAGCCATGT ATGTGCGCTC CCAGCCCGCT CTGTTCGGGG ACATGCTCAG ACTCAGGATA 
GGACTCATCA TGCAAGTGAT GGCCACTGAG TTAGCTCGTA GCCTGCACTG TTCTGGGGAG 
GAGGCGTCAG AGAGTTTGAT GAGCCTGAGT CCATTTGGCA TGAAGAACCT ACTGCATCAC 
ATCCTCAGTG GCAAAGAATT TGGGGTGGAG AGAAGCATGC GCCCAATCCA GTCTACAGCC 
ACTAGCCCTG CGATATCCAT CCATGAACTA GGTCACACGG GAGCTACAAA GACTGAACGC 
ACAGGAATAC ACAAGCTAAA GAGTGAGATA AAACAGCTGG ATGACTCTCG CCCTACAAGT 
CGTTGCAGCA GCCCTTCCAC TCCCAGTGGA ATGCTGTCCC CGGTGAGCAC TTGTCCAGGA 
GACGGTCAGC TGCACTGGGA GGAGAGGCAA GGTCAGTGGC TGAGGAGACG CAGGCTAGAT 
GGTGCCATCA ACAGAGTACC TATGGGTTTC TACCAAAAGG TGTGGAAGAT CCTGCAGAAG 
TGCCACGGTC TGTCCATCGA CGGATACGTG TTGCCCTCTT CTACCACAAG AGAGATGACA 
GCAGGAGAGA TTAAGTTTGC AGTGCAGGTT GAATCTGTGC TGAACCACGT CCCACAGCCA 
GAGTACCGGC AGCTGCTAGT GGAGACTGTG ATGGTTGTGG GCCTGATAGC GGACGTAGAC 
GTGGACAACA TCGGCGGCAT CATCCACGTG GATCGCGTGT TGCATTTGGC CAATGACCTT 
TTCCTCAGTG ACCAGAAATC CCACGGCGCC AGTGAGTATT TCCTTGAGAA GGACCCAGCA 
ACCGGAATCT GCAACTTTTT CTACGACAGC GCTCCTAGTG GTAGCTATGG CACCATGACC 
TATCTCTCCA AAGCGGCAGT CACTTACGTC CAGGACTTCC TGCCAAGTTC CAGCTGCCTG 
ATGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019212374.1
CDS	333..4001
Translation	MRSRSNSGVRLDGYARLVQETILCHQNPVTGLLPASAQKKDAWVRDNVYSVLAVWGLGMAYRKNADRDEDKAKAYELEQSVVKLMQGLLQCMMRQVAKVEKFKHTQSTKDCLHAKYDTPTCATVVRDDQWGHLQVDATSIYLLMLAQMTASGLRIISNLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLFGAHGGPKSVIHVLPDEVEHCQSILCSMLPRASTSKEIDAGLLSITSFPAFAVEDADLVAVTKSEIISKLQGRYGCCRFIRDGYRCPKEDPSRLHYDPAELKLFENIECEWPVFWTYLILDGIFAGDQVQVQEYREALEGVLIRGKNGIKLVPELYSVPPDKVEEEYSNPHSVDRVAMGQLPHMWGQSLYILGSLVAEGFLAPGEIDPLNRRFSTSFKPDVVVQVCVLAESEEIQELLRDLGIEVQTMAEVLPIRVMPARILSHVYVRLGNSKKLNLSGRPYRHIGVLGTSKFYEIRNRSYIFTPQFLDQHHFYLALDNQMIVEMLRTELAYLSCCWRMTGRPTLTFPITRSMLDEDGETIDSCILSTLRKLQDGYFAGARVQMSDVSSVQTTSFHTRLSFLDEENDDILLEDEGDDDDGYGEEYNTCGPSEDSKDVFDQYLTQLLHSTTTKCHLPPIQRGQHHVFSPEHTTRDILSFMAQVQGLNVPKSSMYLPVTPVKSKHRKSLNLLDVPHPQHGPQAKQHKPHSAADLHLPRDSQGNTDFAALVRQLKECPTLQDQADILYILYVMKGADWLVELSGPGQGGVSVRSLLEELYVEAGACKEWALIRYISGILRKRVEVLAEACTDLISHHKQLTVGLPPQPRERVITVPLPPDELNSLIYEASGQDISVAVLTQEIMVYLAMYVRSQPALFGDMLRLRIGLIMQVMATELARSLHCSGEEASESLMSLSPFGMKNLLHHILSGKEFGVERSMRPIQSTATSPAISIHELGHTGATKTERTGIHKLKSEIKQLDDSRPTSRCSSPSTPSGMLSPVSTCPGDGQLHWEERQGQWLRRRRLDGAINRVPMGFYQKVWKILQKCHGLSIDGYVLPSSTTREMTAGEIKFAVQVESVLNHVPQPEYRQLLVETVMVVGLIADVDVDNIGGIIHVDRVLHLANDLFLSDQKSHGASEYFLEKDPATGICNFFYDSAPSGSYGTMTYLSKAAVTYVQDFLPSSSCLMQ

Target ORF information:

RefSeq Version	XM_019356829.1
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus phosphorylase b kinase regulatory subunit alpha, liver isoform (LOC100690562), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019356829.1

ORF Insert Sequence:

ATGAGGAGTC GCAGTAACTC AGGAGTGCGG CTGGACGGCT ATGCCCGGCT GGTCCAGGAG 
ACTATCCTGT GCCATCAGAA CCCAGTGACA GGACTTCTTC CTGCCAGTGC GCAGAAGAAG 
GACGCCTGGG TGAGGGATAA TGTCTACAGT GTCCTGGCGG TGTGGGGGCT GGGTATGGCC 
TACCGGAAGA ATGCAGACCG CGATGAAGAC AAGGCCAAAG CTTACGAACT GGAGCAGAGT 
GTCGTGAAAC TGATGCAGGG GCTTCTGCAG TGCATGATGA GACAGGTGGC CAAGGTGGAG 
AAGTTCAAAC ACACCCAGAG TACAAAGGAT TGCTTGCATG CCAAATATGA TACTCCCACT 
TGTGCCACAG TGGTCAGGGA TGATCAGTGG GGTCATCTCC AGGTGGACGC CACCTCGATT 
TACCTGCTGA TGCTGGCACA GATGACAGCC TCAGGTCTTC GTATCATTTC CAACCTGGAC 
GAGGTAGCCT TCATCCAAAA CTTGGTCTTC TACATAGAGG CTGCCTACAA AGTAGCGGAC 
TATGGAATGT GGGAACGAGG TGACAAGACT AACCAGGGCA TTCCTGAGCT CAATGCCAGC 
TCTGTAGGAA TGGCTAAGGC AGCGCTTGAG GCTATAGATG AGCTGGATCT GTTTGGTGCT 
CACGGAGGGC CGAAGTCAGT CATCCATGTG TTGCCTGATG AAGTCGAACA CTGTCAGTCT 
ATTCTGTGCT CCATGCTGCC AAGAGCTTCA ACTTCAAAGG AAATAGATGC TGGTCTTCTG 
TCCATCACTT CTTTCCCTGC GTTTGCTGTG GAGGATGCTG ACCTGGTGGC TGTGACGAAG 
TCAGAGATCA TAAGCAAACT GCAGGGTCGC TATGGCTGCT GTCGCTTTAT CAGGGACGGA 
TATCGTTGTC CCAAAGAGGA TCCATCTCGG CTGCATTATG ATCCTGCTGA GCTGAAGCTA 
TTTGAGAATA TCGAGTGTGA GTGGCCTGTG TTTTGGACTT ACCTCATCCT GGATGGTATT 
TTTGCTGGTG ATCAAGTTCA GGTGCAAGAG TACCGTGAAG CACTGGAGGG TGTTTTAATC 
AGAGGGAAGA ATGGCATTAA GTTGGTGCCT GAACTTTATT CTGTTCCTCC TGACAAGGTT 
GAGGAGGAGT ATAGCAATCC TCACTCAGTA GACAGGGTAG CCATGGGCCA GCTGCCACAC 
ATGTGGGGAC AGTCACTCTA CATCTTGGGG TCCCTTGTGG CTGAGGGTTT TTTAGCACCA 
GGAGAGATAG ATCCGCTCAA CAGGAGATTC TCTACAAGCT TCAAACCAGA TGTGGTGGTA 
CAAGTTTGTG TTCTAGCAGA GTCGGAGGAG ATCCAGGAGT TGTTAAGAGA TCTGGGGATT 
GAGGTACAGA CGATGGCAGA AGTTCTGCCC ATCAGGGTCA TGCCAGCTCG CATCCTGAGC 
CATGTCTATG TTAGACTGGG GAACAGCAAG AAGCTGAATC TGAGTGGGCG GCCATACAGA 
CACATTGGTG TCCTGGGAAC ATCCAAATTC TACGAGATCC GAAATCGCTC TTACATATTC 
ACCCCTCAGT TTCTGGATCA GCACCATTTC TATCTGGCTC TGGACAACCA GATGATTGTG 
GAGATGTTAC GGACCGAGCT GGCCTATCTC TCTTGTTGCT GGAGGATGAC AGGACGTCCC 
ACGCTCACAT TCCCAATCAC CCGTAGCATG CTGGATGAGG ATGGAGAAAC AATTGATTCA 
TGTATCCTAT CAACTCTAAG GAAACTGCAG GATGGCTATT TTGCTGGAGC GAGGGTGCAG 
ATGTCAGACG TCTCCAGTGT CCAGACCACT TCGTTTCACA CTCGTCTCAG CTTCCTGGAT 
GAAGAGAATG ATGACATATT ACTTGAGGAT GAAGGCGATG ATGATGATGG ATATGGAGAG 
GAGTATAATA CCTGTGGTCC CTCGGAGGAC TCAAAAGATG TATTTGACCA GTACCTCACC 
CAGCTCCTTC ACAGCACCAC TACCAAGTGC CATCTTCCTC CCATCCAGAG GGGGCAGCAC 
CACGTCTTCA GTCCAGAACA TACCACAAGA GACATCCTGT CTTTTATGGC GCAGGTCCAG 
GGCCTGAACG TTCCCAAGTC TTCCATGTAT CTGCCTGTGA CTCCGGTCAA GAGCAAACAT 
CGCAAATCTC TCAACCTTCT TGATGTTCCT CATCCTCAGC ACGGCCCACA GGCGAAACAG 
CACAAGCCAC ACTCTGCTGC TGATCTTCAC CTGCCTCGAG ACTCTCAGGG CAACACAGAC 
TTTGCAGCGT TGGTCAGGCA GCTAAAAGAA TGCCCCACTC TTCAGGACCA GGCTGACATC 
CTCTACATTC TCTATGTGAT GAAAGGAGCT GATTGGCTGG TGGAGTTGTC AGGTCCTGGG 
CAGGGTGGGG TCAGCGTGCG ATCACTGTTG GAGGAGCTGT ACGTAGAAGC CGGAGCCTGC 
AAAGAGTGGG CGCTCATTAG GTACATCTCT GGAATACTGC GCAAGAGGGT GGAGGTCCTC 
GCTGAGGCCT GCACAGATCT GATTTCCCAT CACAAGCAGC TGACTGTAGG TCTACCTCCT 
CAACCCAGGG AAAGAGTGAT CACAGTCCCT CTTCCTCCGG ATGAGTTAAA CAGCCTCATC 
TACGAAGCCA GTGGTCAGGA CATAAGTGTA GCAGTGCTCA CTCAGGAAAT TATGGTGTAT 
CTAGCCATGT ATGTGCGCTC CCAGCCCGCT CTGTTCGGGG ACATGCTCAG ACTCAGGATA 
GGACTCATCA TGCAAGTGAT GGCCACTGAG TTAGCTCGTA GCCTGCACTG TTCTGGGGAG 
GAGGCGTCAG AGAGTTTGAT GAGCCTGAGT CCATTTGGCA TGAAGAACCT ACTGCATCAC 
ATCCTCAGTG GCAAAGAATT TGGGGTGGAG AGAAGCATGC GCCCAATCCA GTCTACAGCC 
ACTAGCCCTG CGATATCCAT CCATGAACTA GGTCACACGG GAGCTACAAA GACTGAACGC 
ACAGGAATAC ACAAGCTAAA GAGTGAGATA AAACAGCTGG ATGACTCTCG CCCTACAAGT 
CGTTGCAGCA GCCCTTCCAC TCCCAGTGGA ATGCTGTCCC CGGTGAGCAC TTGTCCAGGA 
GACGGTCAGC TGCACTGGGA GGAGAGGCAA GGTCAGTGGC TGAGGAGACG CAGGCTAGAT 
GGTGCCATCA ACAGAGTACC TATGGGTTTC TACCAAAAGG TGTGGAAGAT CCTGCAGAAG 
TGCCACGGTC TGTCCATCGA CGGATACGTG TTGCCCTCTT CTACCACAAG AGAGATGACA 
GCAGGAGAGA TTAAGTTTGC AGTGCAGGTT GAATCTGTGC TGAACCACGT CCCACAGCCA 
GAGTACCGGC AGCTGCTAGT GGAGACTGTG ATGGTTGTGG GCCTGATAGC GGACGTAGAC 
GTGGACAACA TCGGCGGCAT CATCCACGTG GATCGCGTGT TGCATTTGGC CAATGACCTT 
TTCCTCAGTG ACCAGAAATC CCACGGCGCC AGTGAGTATT TCCTTGAGAA GGACCCAGCA 
ACCGGAATCT GCAACTTTTT CTACGACAGC GCTCCTAGTG GTAGCTATGG CACCATGACC 
TATCTCTCCA AAGCGGCAGT CACTTACGTC CAGGACTTCC TGCCAAGTTC CAGCTGCCTG 
ATGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100690562 ( XM_019356829.2 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_019212374.1 Oreochromis niloticus phosphorylase b kinase regulatory subunit alpha, liver isoform (LOC100690562), transcript variant X3, mRNA.

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CloneID	OOr28624
Clone ID Related Accession (Same CDS sequence)	XM_019356829.1 , XM_019356829.2
Accession Version	XM_019356829.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031965.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_019356829.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GCAGTAACTC AGGAGTGCGG CTGGACGGCT ATGCCCGGCT GGTCCAGGAG 
ACTATCCTGT GCCATCAGAA CCCAGTGACA GGACTTCTTC CTGCCAGTGC GCAGAAGAAG 
GACGCCTGGG TGAGGGATAA TGTCTACAGT GTCCTGGCGG TGTGGGGGCT GGGTATGGCC 
TACCGGAAGA ATGCAGACCG CGATGAAGAC AAGGCCAAAG CTTACGAACT GGAGCAGAGT 
GTCGTGAAAC TGATGCAGGG GCTTCTGCAG TGCATGATGA GACAGGTGGC CAAGGTGGAG 
AAGTTCAAAC ACACCCAGAG TACAAAGGAT TGCTTGCATG CCAAATATGA TACTCCCACT 
TGTGCCACAG TGGTCAGGGA TGATCAGTGG GGTCATCTCC AGGTGGACGC CACCTCGATT 
TACCTGCTGA TGCTGGCACA GATGACAGCC TCAGGTCTTC GTATCATTTC CAACCTGGAC 
GAGGTAGCCT TCATCCAAAA CTTGGTCTTC TACATAGAGG CTGCCTACAA AGTAGCGGAC 
TATGGAATGT GGGAACGAGG TGACAAGACT AACCAGGGCA TTCCTGAGCT CAATGCCAGC 
TCTGTAGGAA TGGCTAAGGC AGCGCTTGAG GCTATAGATG AGCTGGATCT GTTTGGTGCT 
CACGGAGGGC CGAAGTCAGT CATCCATGTG TTGCCTGATG AAGTCGAACA CTGTCAGTCT 
ATTCTGTGCT CCATGCTGCC AAGAGCTTCA ACTTCAAAGG AAATAGATGC TGGTCTTCTG 
TCCATCACTT CTTTCCCTGC GTTTGCTGTG GAGGATGCTG ACCTGGTGGC TGTGACGAAG 
TCAGAGATCA TAAGCAAACT GCAGGGTCGC TATGGCTGCT GTCGCTTTAT CAGGGACGGA 
TATCGTTGTC CCAAAGAGGA TCCATCTCGG CTGCATTATG ATCCTGCTGA GCTGAAGCTA 
TTTGAGAATA TCGAGTGTGA GTGGCCTGTG TTTTGGACTT ACCTCATCCT GGATGGTATT 
TTTGCTGGTG ATCAAGTTCA GGTGCAAGAG TACCGTGAAG CACTGGAGGG TGTTTTAATC 
AGAGGGAAGA ATGGCATTAA GTTGGTGCCT GAACTTTATT CTGTTCCTCC TGACAAGGTT 
GAGGAGGAGT ATAGCAATCC TCACTCAGTA GACAGGGTAG CCATGGGCCA GCTGCCACAC 
ATGTGGGGAC AGTCACTCTA CATCTTGGGG TCCCTTGTGG CTGAGGGTTT TTTAGCACCA 
GGAGAGATAG ATCCGCTCAA CAGGAGATTC TCTACAAGCT TCAAACCAGA TGTGGTGGTA 
CAAGTTTGTG TTCTAGCAGA GTCGGAGGAG ATCCAGGAGT TGTTAAGAGA TCTGGGGATT 
GAGGTACAGA CGATGGCAGA AGTTCTGCCC ATCAGGGTCA TGCCAGCTCG CATCCTGAGC 
CATGTCTATG TTAGACTGGG GAACAGCAAG AAGCTGAATC TGAGTGGGCG GCCATACAGA 
CACATTGGTG TCCTGGGAAC ATCCAAATTC TACGAGATCC GAAATCGCTC TTACATATTC 
ACCCCTCAGT TTCTGGATCA GCACCATTTC TATCTGGCTC TGGACAACCA GATGATTGTG 
GAGATGTTAC GGACCGAGCT GGCCTATCTC TCTTGTTGCT GGAGGATGAC AGGACGTCCC 
ACGCTCACAT TCCCAATCAC CCGTAGCATG CTGGATGAGG ATGGAGAAAC AATTGATTCA 
TGTATCCTAT CAACTCTAAG GAAACTGCAG GATGGCTATT TTGCTGGAGC GAGGGTGCAG 
ATGTCAGACG TCTCCAGTGT CCAGACCACT TCGTTTCACA CTCGTCTCAG CTTCCTGGAT 
GAAGAGAATG ATGACATATT ACTTGAGGAT GAAGGCGATG ATGATGATGG ATATGGAGAG 
GAGTATAATA CCTGTGGTCC CTCGGAGGAC TCAAAAGATG TATTTGACCA GTACCTCACC 
CAGCTCCTTC ACAGCACCAC TACCAAGTGC CATCTTCCTC CCATCCAGAG GGGGCAGCAC 
CACGTCTTCA GTCCAGAACA TACCACAAGA GACATCCTGT CTTTTATGGC GCAGGTCCAG 
GGCCTGAACG TTCCCAAGTC TTCCATGTAT CTGCCTGTGA CTCCGGTCAA GAGCAAACAT 
CGCAAATCTC TCAACCTTCT TGATGTTCCT CATCCTCAGC ACGGCCCACA GGCGAAACAG 
CACAAGCCAC ACTCTGCTGC TGATCTTCAC CTGCCTCGAG ACTCTCAGGG CAACACAGAC 
TTTGCAGCGT TGGTCAGGCA GCTAAAAGAA TGCCCCACTC TTCAGGACCA GGCTGACATC 
CTCTACATTC TCTATGTGAT GAAAGGAGCT GATTGGCTGG TGGAGTTGTC AGGTCCTGGG 
CAGGGTGGGG TCAGCGTGCG ATCACTGTTG GAGGAGCTGT ACGTAGAAGC CGGAGCCTGC 
AAAGAGTGGG CGCTCATTAG GTACATCTCT GGAATACTGC GCAAGAGGGT GGAGGTCCTC 
GCTGAGGCCT GCACAGATCT GATTTCCCAT CACAAGCAGC TGACTGTAGG TCTACCTCCT 
CAACCCAGGG AAAGAGTGAT CACAGTCCCT CTTCCTCCGG ATGAGTTAAA CAGCCTCATC 
TACGAAGCCA GTGGTCAGGA CATAAGTGTA GCAGTGCTCA CTCAGGAAAT TATGGTGTAT 
CTAGCCATGT ATGTGCGCTC CCAGCCCGCT CTGTTCGGGG ACATGCTCAG ACTCAGGATA 
GGACTCATCA TGCAAGTGAT GGCCACTGAG TTAGCTCGTA GCCTGCACTG TTCTGGGGAG 
GAGGCGTCAG AGAGTTTGAT GAGCCTGAGT CCATTTGGCA TGAAGAACCT ACTGCATCAC 
ATCCTCAGTG GCAAAGAATT TGGGGTGGAG AGAAGCATGC GCCCAATCCA GTCTACAGCC 
ACTAGCCCTG CGATATCCAT CCATGAACTA GGTCACACGG GAGCTACAAA GACTGAACGC 
ACAGGAATAC ACAAGCTAAA GAGTGAGATA AAACAGCTGG ATGACTCTCG CCCTACAAGT 
CGTTGCAGCA GCCCTTCCAC TCCCAGTGGA ATGCTGTCCC CGGTGAGCAC TTGTCCAGGA 
GACGGTCAGC TGCACTGGGA GGAGAGGCAA GGTCAGTGGC TGAGGAGACG CAGGCTAGAT 
GGTGCCATCA ACAGAGTACC TATGGGTTTC TACCAAAAGG TGTGGAAGAT CCTGCAGAAG 
TGCCACGGTC TGTCCATCGA CGGATACGTG TTGCCCTCTT CTACCACAAG AGAGATGACA 
GCAGGAGAGA TTAAGTTTGC AGTGCAGGTT GAATCTGTGC TGAACCACGT CCCACAGCCA 
GAGTACCGGC AGCTGCTAGT GGAGACTGTG ATGGTTGTGG GCCTGATAGC GGACGTAGAC 
GTGGACAACA TCGGCGGCAT CATCCACGTG GATCGCGTGT TGCATTTGGC CAATGACCTT 
TTCCTCAGTG ACCAGAAATC CCACGGCGCC AGTGAGTATT TCCTTGAGAA GGACCCAGCA 
ACCGGAATCT GCAACTTTTT CTACGACAGC GCTCCTAGTG GTAGCTATGG CACCATGACC 
TATCTCTCCA AAGCGGCAGT CACTTACGTC CAGGACTTCC TGCCAAGTTC CAGCTGCCTG 
ATGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019212374.1
CDS	309..3977
Translation	MRSRSNSGVRLDGYARLVQETILCHQNPVTGLLPASAQKKDAWVRDNVYSVLAVWGLGMAYRKNADRDEDKAKAYELEQSVVKLMQGLLQCMMRQVAKVEKFKHTQSTKDCLHAKYDTPTCATVVRDDQWGHLQVDATSIYLLMLAQMTASGLRIISNLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLFGAHGGPKSVIHVLPDEVEHCQSILCSMLPRASTSKEIDAGLLSITSFPAFAVEDADLVAVTKSEIISKLQGRYGCCRFIRDGYRCPKEDPSRLHYDPAELKLFENIECEWPVFWTYLILDGIFAGDQVQVQEYREALEGVLIRGKNGIKLVPELYSVPPDKVEEEYSNPHSVDRVAMGQLPHMWGQSLYILGSLVAEGFLAPGEIDPLNRRFSTSFKPDVVVQVCVLAESEEIQELLRDLGIEVQTMAEVLPIRVMPARILSHVYVRLGNSKKLNLSGRPYRHIGVLGTSKFYEIRNRSYIFTPQFLDQHHFYLALDNQMIVEMLRTELAYLSCCWRMTGRPTLTFPITRSMLDEDGETIDSCILSTLRKLQDGYFAGARVQMSDVSSVQTTSFHTRLSFLDEENDDILLEDEGDDDDGYGEEYNTCGPSEDSKDVFDQYLTQLLHSTTTKCHLPPIQRGQHHVFSPEHTTRDILSFMAQVQGLNVPKSSMYLPVTPVKSKHRKSLNLLDVPHPQHGPQAKQHKPHSAADLHLPRDSQGNTDFAALVRQLKECPTLQDQADILYILYVMKGADWLVELSGPGQGGVSVRSLLEELYVEAGACKEWALIRYISGILRKRVEVLAEACTDLISHHKQLTVGLPPQPRERVITVPLPPDELNSLIYEASGQDISVAVLTQEIMVYLAMYVRSQPALFGDMLRLRIGLIMQVMATELARSLHCSGEEASESLMSLSPFGMKNLLHHILSGKEFGVERSMRPIQSTATSPAISIHELGHTGATKTERTGIHKLKSEIKQLDDSRPTSRCSSPSTPSGMLSPVSTCPGDGQLHWEERQGQWLRRRRLDGAINRVPMGFYQKVWKILQKCHGLSIDGYVLPSSTTREMTAGEIKFAVQVESVLNHVPQPEYRQLLVETVMVVGLIADVDVDNIGGIIHVDRVLHLANDLFLSDQKSHGASEYFLEKDPATGICNFFYDSAPSGSYGTMTYLSKAAVTYVQDFLPSSSCLMQ

Target ORF information:

RefSeq Version	XM_019356829.2
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus phosphorylase b kinase regulatory subunit alpha, liver isoform (LOC100690562), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019356829.2

ORF Insert Sequence:

ATGAGGAGTC GCAGTAACTC AGGAGTGCGG CTGGACGGCT ATGCCCGGCT GGTCCAGGAG 
ACTATCCTGT GCCATCAGAA CCCAGTGACA GGACTTCTTC CTGCCAGTGC GCAGAAGAAG 
GACGCCTGGG TGAGGGATAA TGTCTACAGT GTCCTGGCGG TGTGGGGGCT GGGTATGGCC 
TACCGGAAGA ATGCAGACCG CGATGAAGAC AAGGCCAAAG CTTACGAACT GGAGCAGAGT 
GTCGTGAAAC TGATGCAGGG GCTTCTGCAG TGCATGATGA GACAGGTGGC CAAGGTGGAG 
AAGTTCAAAC ACACCCAGAG TACAAAGGAT TGCTTGCATG CCAAATATGA TACTCCCACT 
TGTGCCACAG TGGTCAGGGA TGATCAGTGG GGTCATCTCC AGGTGGACGC CACCTCGATT 
TACCTGCTGA TGCTGGCACA GATGACAGCC TCAGGTCTTC GTATCATTTC CAACCTGGAC 
GAGGTAGCCT TCATCCAAAA CTTGGTCTTC TACATAGAGG CTGCCTACAA AGTAGCGGAC 
TATGGAATGT GGGAACGAGG TGACAAGACT AACCAGGGCA TTCCTGAGCT CAATGCCAGC 
TCTGTAGGAA TGGCTAAGGC AGCGCTTGAG GCTATAGATG AGCTGGATCT GTTTGGTGCT 
CACGGAGGGC CGAAGTCAGT CATCCATGTG TTGCCTGATG AAGTCGAACA CTGTCAGTCT 
ATTCTGTGCT CCATGCTGCC AAGAGCTTCA ACTTCAAAGG AAATAGATGC TGGTCTTCTG 
TCCATCACTT CTTTCCCTGC GTTTGCTGTG GAGGATGCTG ACCTGGTGGC TGTGACGAAG 
TCAGAGATCA TAAGCAAACT GCAGGGTCGC TATGGCTGCT GTCGCTTTAT CAGGGACGGA 
TATCGTTGTC CCAAAGAGGA TCCATCTCGG CTGCATTATG ATCCTGCTGA GCTGAAGCTA 
TTTGAGAATA TCGAGTGTGA GTGGCCTGTG TTTTGGACTT ACCTCATCCT GGATGGTATT 
TTTGCTGGTG ATCAAGTTCA GGTGCAAGAG TACCGTGAAG CACTGGAGGG TGTTTTAATC 
AGAGGGAAGA ATGGCATTAA GTTGGTGCCT GAACTTTATT CTGTTCCTCC TGACAAGGTT 
GAGGAGGAGT ATAGCAATCC TCACTCAGTA GACAGGGTAG CCATGGGCCA GCTGCCACAC 
ATGTGGGGAC AGTCACTCTA CATCTTGGGG TCCCTTGTGG CTGAGGGTTT TTTAGCACCA 
GGAGAGATAG ATCCGCTCAA CAGGAGATTC TCTACAAGCT TCAAACCAGA TGTGGTGGTA 
CAAGTTTGTG TTCTAGCAGA GTCGGAGGAG ATCCAGGAGT TGTTAAGAGA TCTGGGGATT 
GAGGTACAGA CGATGGCAGA AGTTCTGCCC ATCAGGGTCA TGCCAGCTCG CATCCTGAGC 
CATGTCTATG TTAGACTGGG GAACAGCAAG AAGCTGAATC TGAGTGGGCG GCCATACAGA 
CACATTGGTG TCCTGGGAAC ATCCAAATTC TACGAGATCC GAAATCGCTC TTACATATTC 
ACCCCTCAGT TTCTGGATCA GCACCATTTC TATCTGGCTC TGGACAACCA GATGATTGTG 
GAGATGTTAC GGACCGAGCT GGCCTATCTC TCTTGTTGCT GGAGGATGAC AGGACGTCCC 
ACGCTCACAT TCCCAATCAC CCGTAGCATG CTGGATGAGG ATGGAGAAAC AATTGATTCA 
TGTATCCTAT CAACTCTAAG GAAACTGCAG GATGGCTATT TTGCTGGAGC GAGGGTGCAG 
ATGTCAGACG TCTCCAGTGT CCAGACCACT TCGTTTCACA CTCGTCTCAG CTTCCTGGAT 
GAAGAGAATG ATGACATATT ACTTGAGGAT GAAGGCGATG ATGATGATGG ATATGGAGAG 
GAGTATAATA CCTGTGGTCC CTCGGAGGAC TCAAAAGATG TATTTGACCA GTACCTCACC 
CAGCTCCTTC ACAGCACCAC TACCAAGTGC CATCTTCCTC CCATCCAGAG GGGGCAGCAC 
CACGTCTTCA GTCCAGAACA TACCACAAGA GACATCCTGT CTTTTATGGC GCAGGTCCAG 
GGCCTGAACG TTCCCAAGTC TTCCATGTAT CTGCCTGTGA CTCCGGTCAA GAGCAAACAT 
CGCAAATCTC TCAACCTTCT TGATGTTCCT CATCCTCAGC ACGGCCCACA GGCGAAACAG 
CACAAGCCAC ACTCTGCTGC TGATCTTCAC CTGCCTCGAG ACTCTCAGGG CAACACAGAC 
TTTGCAGCGT TGGTCAGGCA GCTAAAAGAA TGCCCCACTC TTCAGGACCA GGCTGACATC 
CTCTACATTC TCTATGTGAT GAAAGGAGCT GATTGGCTGG TGGAGTTGTC AGGTCCTGGG 
CAGGGTGGGG TCAGCGTGCG ATCACTGTTG GAGGAGCTGT ACGTAGAAGC CGGAGCCTGC 
AAAGAGTGGG CGCTCATTAG GTACATCTCT GGAATACTGC GCAAGAGGGT GGAGGTCCTC 
GCTGAGGCCT GCACAGATCT GATTTCCCAT CACAAGCAGC TGACTGTAGG TCTACCTCCT 
CAACCCAGGG AAAGAGTGAT CACAGTCCCT CTTCCTCCGG ATGAGTTAAA CAGCCTCATC 
TACGAAGCCA GTGGTCAGGA CATAAGTGTA GCAGTGCTCA CTCAGGAAAT TATGGTGTAT 
CTAGCCATGT ATGTGCGCTC CCAGCCCGCT CTGTTCGGGG ACATGCTCAG ACTCAGGATA 
GGACTCATCA TGCAAGTGAT GGCCACTGAG TTAGCTCGTA GCCTGCACTG TTCTGGGGAG 
GAGGCGTCAG AGAGTTTGAT GAGCCTGAGT CCATTTGGCA TGAAGAACCT ACTGCATCAC 
ATCCTCAGTG GCAAAGAATT TGGGGTGGAG AGAAGCATGC GCCCAATCCA GTCTACAGCC 
ACTAGCCCTG CGATATCCAT CCATGAACTA GGTCACACGG GAGCTACAAA GACTGAACGC 
ACAGGAATAC ACAAGCTAAA GAGTGAGATA AAACAGCTGG ATGACTCTCG CCCTACAAGT 
CGTTGCAGCA GCCCTTCCAC TCCCAGTGGA ATGCTGTCCC CGGTGAGCAC TTGTCCAGGA 
GACGGTCAGC TGCACTGGGA GGAGAGGCAA GGTCAGTGGC TGAGGAGACG CAGGCTAGAT 
GGTGCCATCA ACAGAGTACC TATGGGTTTC TACCAAAAGG TGTGGAAGAT CCTGCAGAAG 
TGCCACGGTC TGTCCATCGA CGGATACGTG TTGCCCTCTT CTACCACAAG AGAGATGACA 
GCAGGAGAGA TTAAGTTTGC AGTGCAGGTT GAATCTGTGC TGAACCACGT CCCACAGCCA 
GAGTACCGGC AGCTGCTAGT GGAGACTGTG ATGGTTGTGG GCCTGATAGC GGACGTAGAC 
GTGGACAACA TCGGCGGCAT CATCCACGTG GATCGCGTGT TGCATTTGGC CAATGACCTT 
TTCCTCAGTG ACCAGAAATC CCACGGCGCC AGTGAGTATT TCCTTGAGAA GGACCCAGCA 
ACCGGAATCT GCAACTTTTT CTACGACAGC GCTCCTAGTG GTAGCTATGG CACCATGACC 
TATCTCTCCA AAGCGGCAGT CACTTACGTC CAGGACTTCC TGCCAAGTTC CAGCTGCCTG 
ATGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100690562 ( XM_019356832.1 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_019212377.1 Oreochromis niloticus phosphorylase b kinase regulatory subunit alpha, liver isoform (LOC100690562), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOr28625
Clone ID Related Accession (Same CDS sequence)	XM_019356832.1 , XM_019356832.2
Accession Version	XM_019356832.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031965.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACAG TGTCCTGGCG GTGTGGGGGC TGGGTATGGC CTACCGGAAG AATGCAGACC 
GCGATGAAGA CAAGGCCAAA GCTTACGAAC TGGAGCAGGT GTGTTTGCAA CATAAATAGA 
AGTGTCGTGA AACTGATGCA GGGGCTTCTG CAGTGCATGA TGAGACAGGT GGCCAAGGTG 
GAGAAGTTCA AACACACCCA GAGTACAAAG GATTGCTTGC ATGCCAAATA TGATACTCCC 
ACTTGTGCCA CAGTGGTCAG GGATGATCAG TGGGGTCATC TCCAGGTGGA CGCCACCTCG 
ATTTACCTGC TGATGCTGGC ACAGATGACA GCCTCAGGTC TTCGTATCAT TTCCAACCTG 
GACGAGGTAG CCTTCATCCA AAACTTGGTC TTCTACATAG AGGCTGCCTA CAAAGTAGCG 
GACTATGGAA TGTGGGAACG AGGTGACAAG ACTAACCAGG GCATTCCTGA GCTCAATGCC 
AGCTCTGTAG GAATGGCTAA GGCAGCGCTT GAGGCTATAG ATGAGCTGGA TCTGTTTGGT 
GCTCACGGAG GGCCGAAGTC AGTCATCCAT GTGTTGCCTG ATGAAGTCGA ACACTGTCAG 
TCTATTCTGT GCTCCATGCT GCCAAGAGCT TCAACTTCAA AGGAAATAGA TGCTGGTCTT 
CTGTCCATCA CTTCTTTCCC TGCGTTTGCT GTGGAGGATG CTGACCTGGT GGCTGTGACG 
AAGTCAGAGA TCATAAGCAA ACTGCAGGGT CGCTATGGCT GCTGTCGCTT TATCAGGGAC 
GGATATCGTT GTCCCAAAGA GGATCCATCT CGGCTGCATT ATGATCCTGC TGAGCTGAAG 
CTATTTGAGA ATATCGAGTG TGAGTGGCCT GTGTTTTGGA CTTACCTCAT CCTGGATGGT 
ATTTTTGCTG GTGATCAAGT TCAGGTGCAA GAGTACCGTG AAGCACTGGA GGGTGTTTTA 
ATCAGAGGGA AGAATGGCAT TAAGTTGGTG CCTGAACTTT ATTCTGTTCC TCCTGACAAG 
GTTGAGGAGG AGTATAGCAA TCCTCACTCA GTAGACAGGG TAGCCATGGG CCAGCTGCCA 
CACATGTGGG GACAGTCACT CTACATCTTG GGGTCCCTTG TGGCTGAGGG TTTTTTAGCA 
CCAGGAGAGA TAGATCCGCT CAACAGGAGA TTCTCTACAA GCTTCAAACC AGATGTGGTG 
GTACAAGTTT GTGTTCTAGC AGAGTCGGAG GAGATCCAGG AGTTGTTAAG AGATCTGGGG 
ATTGAGGTAC AGACGATGGC AGAAGTTCTG CCCATCAGGG TCATGCCAGC TCGCATCCTG 
AGCCATGTCT ATGTTAGACT GGGGAACAGC AAGAAGCTGA ATCTGAGTGG GCGGCCATAC 
AGACACATTG GTGTCCTGGG AACATCCAAA TTCTACGAGA TCCGAAATCG CTCTTACATA 
TTCACCCCTC AGTTTCTGGA TCAGCACCAT TTCTATCTGG CTCTGGACAA CCAGATGATT 
GTGGAGATGT TACGGACCGA GCTGGCCTAT CTCTCTTGTT GCTGGAGGAT GACAGGACGT 
CCCACGCTCA CATTCCCAAT CACCCGTAGC ATGCTGGATG AGGATGGAGA AACAATTGAT 
TCATGTATCC TATCAACTCT AAGGAAACTG CAGGATGGCT ATTTTGCTGG AGCGAGGGTG 
CAGATGTCAG ACGTCTCCAG TGTCCAGACC ACTTCGTTTC ACACTCGTCT CAGCTTCCTG 
GATGAAGAGA ATGATGACAT ATTACTTGAG GATGAAGGCG ATGATGATGA TGGATATGGA 
GAGGAGTATA ATACCTGTGG TCCCTCGGAG GACTCAAAAG ATGTATTTGA CCAGTACCTC 
ACCCAGCTCC TTCACAGCAC CACTACCAAG TGCCATCTTC CTCCCATCCA GAGGGGGCAG 
CACCACGTCT TCAGTCCAGA ACATACCACA AGAGACATCC TGTCTTTTAT GGCGCAGGTC 
CAGGGCCTGA ACGTTCCCAA GTCTTCCATG TATCTGCCTG TGACTCCGGT CAAGAGCAAA 
CATCGCAAAT CTCTCAACCT TCTTGATGTT CCTCATCCTC AGCACGGCCC ACAGGCGAAA 
CAGCACAAGC CACACTCTGC TGCTGATCTT CACCTGCCTC GAGACTCTCA GGGCAACACA 
GACTTTGCAG CGTTGGTCAG GCAGCTAAAA GAATGCCCCA CTCTTCAGGA CCAGGCTGAC 
ATCCTCTACA TTCTCTATGT GATGAAAGGA GCTGATTGGC TGGTGGAGTT GTCAGGTCCT 
GGGCAGGGTG GGGTCAGCGT GCGATCACTG TTGGAGGAGC TGTACGTAGA AGCCGGAGCC 
TGCAAAGAGT GGGCGCTCAT TAGGTACATC TCTGGAATAC TGCGCAAGAG GGTGGAGGTC 
CTCGCTGAGG CCTGCACAGA TCTGATTTCC CATCACAAGC AGCTGACTGT AGGTCTACCT 
CCTCAACCCA GGGAAAGAGT GATCACAGTC CCTCTTCCTC CGGATGAGTT AAACAGCCTC 
ATCTACGAAG CCAGTGGTCA GGACATAAGT GTAGCAGTGC TCACTCAGGA AATTATGGTG 
TATCTAGCCA TGTATGTGCG CTCCCAGCCC GCTCTGTTCG GGGACATGCT CAGACTCAGG 
ATAGGACTCA TCATGCAAGT GATGGCCACT GAGTTAGCTC GTAGCCTGCA CTGTTCTGGG 
GAGGAGGCGT CAGAGAGTTT GATGAGCCTG AGTCCATTTG GCATGAAGAA CCTACTGCAT 
CACATCCTCA GTGGCAAAGA ATTTGGGGTG GAGAGAAGCA TGCGCCCAAT CCAGTCTACA 
GCCACTAGCC CTGCGATATC CATCCATGAA CTAGGTCACA CGGGAGCTAC AAAGACTGAA 
CGCACAGGAA TACACAAGCT AAAGAGTGAG ATAAAACAGC TGGATGACTC TCGCCCTACA 
AGTATCTTCA GTGGCGGTTT TTCCTTGAGT AGCAATGTCA CATCTCCTCG CTCTACGCGT 
TGCAGCAGCC CTTCCACTCC CAGTGGAATG CTGTCCCCGG TGAGCACTTG TCCAGGAGAC 
GGTCAGCTGC ACTGGGAGGA GAGGCAAGGT CAGTGGCTGA GGAGACGCAG GCTAGATGGT 
GCCATCAACA GAGTACCTAT GGGTTTCTAC CAAAAGGTGT GGAAGATCCT GCAGAAGTGC 
CACGGTCTGT CCATCGACGG ATACGTGTTG CCCTCTTCTA CCACAAGAGA GATGACAGCA 
GGAGAGATTA AGTTTGCAGT GCAGGTTGAA TCTGTGCTGA ACCACGTCCC ACAGCCAGAG 
TACCGGCAGC TGCTAGTGGA GACTGTGATG GTTGTGGGCC TGATAGCGGA CGTAGACGTG 
GACAACATCG GCGGCATCAT CCACGTGGAT CGCGTGTTGC ATTTGGCCAA TGACCTTTTC 
CTCAGTGACC AGAAATCCCA CGGCGCCAGT GAGTATTTCC TTGAGAAGGA CCCAGCAACC 
GGAATCTGCA ACTTTTTCTA CGACAGCGCT CCTAGTGGTA GCTATGGCAC CATGACCTAT 
CTCTCCAAAG CGGCAGTCAC TTACGTCCAG GACTTCCTGC CAAGTTCCAG CTGCCTGATG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019212377.1
CDS	16..3621
Translation	MSTVSWRCGGWVWPTGRMQTAMKTRPKLTNWSRCVCNINRSVVKLMQGLLQCMMRQVAKVEKFKHTQSTKDCLHAKYDTPTCATVVRDDQWGHLQVDATSIYLLMLAQMTASGLRIISNLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLFGAHGGPKSVIHVLPDEVEHCQSILCSMLPRASTSKEIDAGLLSITSFPAFAVEDADLVAVTKSEIISKLQGRYGCCRFIRDGYRCPKEDPSRLHYDPAELKLFENIECEWPVFWTYLILDGIFAGDQVQVQEYREALEGVLIRGKNGIKLVPELYSVPPDKVEEEYSNPHSVDRVAMGQLPHMWGQSLYILGSLVAEGFLAPGEIDPLNRRFSTSFKPDVVVQVCVLAESEEIQELLRDLGIEVQTMAEVLPIRVMPARILSHVYVRLGNSKKLNLSGRPYRHIGVLGTSKFYEIRNRSYIFTPQFLDQHHFYLALDNQMIVEMLRTELAYLSCCWRMTGRPTLTFPITRSMLDEDGETIDSCILSTLRKLQDGYFAGARVQMSDVSSVQTTSFHTRLSFLDEENDDILLEDEGDDDDGYGEEYNTCGPSEDSKDVFDQYLTQLLHSTTTKCHLPPIQRGQHHVFSPEHTTRDILSFMAQVQGLNVPKSSMYLPVTPVKSKHRKSLNLLDVPHPQHGPQAKQHKPHSAADLHLPRDSQGNTDFAALVRQLKECPTLQDQADILYILYVMKGADWLVELSGPGQGGVSVRSLLEELYVEAGACKEWALIRYISGILRKRVEVLAEACTDLISHHKQLTVGLPPQPRERVITVPLPPDELNSLIYEASGQDISVAVLTQEIMVYLAMYVRSQPALFGDMLRLRIGLIMQVMATELARSLHCSGEEASESLMSLSPFGMKNLLHHILSGKEFGVERSMRPIQSTATSPAISIHELGHTGATKTERTGIHKLKSEIKQLDDSRPTSIFSGGFSLSSNVTSPRSTRCSSPSTPSGMLSPVSTCPGDGQLHWEERQGQWLRRRRLDGAINRVPMGFYQKVWKILQKCHGLSIDGYVLPSSTTREMTAGEIKFAVQVESVLNHVPQPEYRQLLVETVMVVGLIADVDVDNIGGIIHVDRVLHLANDLFLSDQKSHGASEYFLEKDPATGICNFFYDSAPSGSYGTMTYLSKAAVTYVQDFLPSSSCLMQ

Target ORF information:

RefSeq Version	XM_019356832.1
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus phosphorylase b kinase regulatory subunit alpha, liver isoform (LOC100690562), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019356832.1

ORF Insert Sequence:

ATGTCTACAG TGTCCTGGCG GTGTGGGGGC TGGGTATGGC CTACCGGAAG AATGCAGACC 
GCGATGAAGA CAAGGCCAAA GCTTACGAAC TGGAGCAGGT GTGTTTGCAA CATAAATAGA 
AGTGTCGTGA AACTGATGCA GGGGCTTCTG CAGTGCATGA TGAGACAGGT GGCCAAGGTG 
GAGAAGTTCA AACACACCCA GAGTACAAAG GATTGCTTGC ATGCCAAATA TGATACTCCC 
ACTTGTGCCA CAGTGGTCAG GGATGATCAG TGGGGTCATC TCCAGGTGGA CGCCACCTCG 
ATTTACCTGC TGATGCTGGC ACAGATGACA GCCTCAGGTC TTCGTATCAT TTCCAACCTG 
GACGAGGTAG CCTTCATCCA AAACTTGGTC TTCTACATAG AGGCTGCCTA CAAAGTAGCG 
GACTATGGAA TGTGGGAACG AGGTGACAAG ACTAACCAGG GCATTCCTGA GCTCAATGCC 
AGCTCTGTAG GAATGGCTAA GGCAGCGCTT GAGGCTATAG ATGAGCTGGA TCTGTTTGGT 
GCTCACGGAG GGCCGAAGTC AGTCATCCAT GTGTTGCCTG ATGAAGTCGA ACACTGTCAG 
TCTATTCTGT GCTCCATGCT GCCAAGAGCT TCAACTTCAA AGGAAATAGA TGCTGGTCTT 
CTGTCCATCA CTTCTTTCCC TGCGTTTGCT GTGGAGGATG CTGACCTGGT GGCTGTGACG 
AAGTCAGAGA TCATAAGCAA ACTGCAGGGT CGCTATGGCT GCTGTCGCTT TATCAGGGAC 
GGATATCGTT GTCCCAAAGA GGATCCATCT CGGCTGCATT ATGATCCTGC TGAGCTGAAG 
CTATTTGAGA ATATCGAGTG TGAGTGGCCT GTGTTTTGGA CTTACCTCAT CCTGGATGGT 
ATTTTTGCTG GTGATCAAGT TCAGGTGCAA GAGTACCGTG AAGCACTGGA GGGTGTTTTA 
ATCAGAGGGA AGAATGGCAT TAAGTTGGTG CCTGAACTTT ATTCTGTTCC TCCTGACAAG 
GTTGAGGAGG AGTATAGCAA TCCTCACTCA GTAGACAGGG TAGCCATGGG CCAGCTGCCA 
CACATGTGGG GACAGTCACT CTACATCTTG GGGTCCCTTG TGGCTGAGGG TTTTTTAGCA 
CCAGGAGAGA TAGATCCGCT CAACAGGAGA TTCTCTACAA GCTTCAAACC AGATGTGGTG 
GTACAAGTTT GTGTTCTAGC AGAGTCGGAG GAGATCCAGG AGTTGTTAAG AGATCTGGGG 
ATTGAGGTAC AGACGATGGC AGAAGTTCTG CCCATCAGGG TCATGCCAGC TCGCATCCTG 
AGCCATGTCT ATGTTAGACT GGGGAACAGC AAGAAGCTGA ATCTGAGTGG GCGGCCATAC 
AGACACATTG GTGTCCTGGG AACATCCAAA TTCTACGAGA TCCGAAATCG CTCTTACATA 
TTCACCCCTC AGTTTCTGGA TCAGCACCAT TTCTATCTGG CTCTGGACAA CCAGATGATT 
GTGGAGATGT TACGGACCGA GCTGGCCTAT CTCTCTTGTT GCTGGAGGAT GACAGGACGT 
CCCACGCTCA CATTCCCAAT CACCCGTAGC ATGCTGGATG AGGATGGAGA AACAATTGAT 
TCATGTATCC TATCAACTCT AAGGAAACTG CAGGATGGCT ATTTTGCTGG AGCGAGGGTG 
CAGATGTCAG ACGTCTCCAG TGTCCAGACC ACTTCGTTTC ACACTCGTCT CAGCTTCCTG 
GATGAAGAGA ATGATGACAT ATTACTTGAG GATGAAGGCG ATGATGATGA TGGATATGGA 
GAGGAGTATA ATACCTGTGG TCCCTCGGAG GACTCAAAAG ATGTATTTGA CCAGTACCTC 
ACCCAGCTCC TTCACAGCAC CACTACCAAG TGCCATCTTC CTCCCATCCA GAGGGGGCAG 
CACCACGTCT TCAGTCCAGA ACATACCACA AGAGACATCC TGTCTTTTAT GGCGCAGGTC 
CAGGGCCTGA ACGTTCCCAA GTCTTCCATG TATCTGCCTG TGACTCCGGT CAAGAGCAAA 
CATCGCAAAT CTCTCAACCT TCTTGATGTT CCTCATCCTC AGCACGGCCC ACAGGCGAAA 
CAGCACAAGC CACACTCTGC TGCTGATCTT CACCTGCCTC GAGACTCTCA GGGCAACACA 
GACTTTGCAG CGTTGGTCAG GCAGCTAAAA GAATGCCCCA CTCTTCAGGA CCAGGCTGAC 
ATCCTCTACA TTCTCTATGT GATGAAAGGA GCTGATTGGC TGGTGGAGTT GTCAGGTCCT 
GGGCAGGGTG GGGTCAGCGT GCGATCACTG TTGGAGGAGC TGTACGTAGA AGCCGGAGCC 
TGCAAAGAGT GGGCGCTCAT TAGGTACATC TCTGGAATAC TGCGCAAGAG GGTGGAGGTC 
CTCGCTGAGG CCTGCACAGA TCTGATTTCC CATCACAAGC AGCTGACTGT AGGTCTACCT 
CCTCAACCCA GGGAAAGAGT GATCACAGTC CCTCTTCCTC CGGATGAGTT AAACAGCCTC 
ATCTACGAAG CCAGTGGTCA GGACATAAGT GTAGCAGTGC TCACTCAGGA AATTATGGTG 
TATCTAGCCA TGTATGTGCG CTCCCAGCCC GCTCTGTTCG GGGACATGCT CAGACTCAGG 
ATAGGACTCA TCATGCAAGT GATGGCCACT GAGTTAGCTC GTAGCCTGCA CTGTTCTGGG 
GAGGAGGCGT CAGAGAGTTT GATGAGCCTG AGTCCATTTG GCATGAAGAA CCTACTGCAT 
CACATCCTCA GTGGCAAAGA ATTTGGGGTG GAGAGAAGCA TGCGCCCAAT CCAGTCTACA 
GCCACTAGCC CTGCGATATC CATCCATGAA CTAGGTCACA CGGGAGCTAC AAAGACTGAA 
CGCACAGGAA TACACAAGCT AAAGAGTGAG ATAAAACAGC TGGATGACTC TCGCCCTACA 
AGTATCTTCA GTGGCGGTTT TTCCTTGAGT AGCAATGTCA CATCTCCTCG CTCTACGCGT 
TGCAGCAGCC CTTCCACTCC CAGTGGAATG CTGTCCCCGG TGAGCACTTG TCCAGGAGAC 
GGTCAGCTGC ACTGGGAGGA GAGGCAAGGT CAGTGGCTGA GGAGACGCAG GCTAGATGGT 
GCCATCAACA GAGTACCTAT GGGTTTCTAC CAAAAGGTGT GGAAGATCCT GCAGAAGTGC 
CACGGTCTGT CCATCGACGG ATACGTGTTG CCCTCTTCTA CCACAAGAGA GATGACAGCA 
GGAGAGATTA AGTTTGCAGT GCAGGTTGAA TCTGTGCTGA ACCACGTCCC ACAGCCAGAG 
TACCGGCAGC TGCTAGTGGA GACTGTGATG GTTGTGGGCC TGATAGCGGA CGTAGACGTG 
GACAACATCG GCGGCATCAT CCACGTGGAT CGCGTGTTGC ATTTGGCCAA TGACCTTTTC 
CTCAGTGACC AGAAATCCCA CGGCGCCAGT GAGTATTTCC TTGAGAAGGA CCCAGCAACC 
GGAATCTGCA ACTTTTTCTA CGACAGCGCT CCTAGTGGTA GCTATGGCAC CATGACCTAT 
CTCTCCAAAG CGGCAGTCAC TTACGTCCAG GACTTCCTGC CAAGTTCCAG CTGCCTGATG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100690562 ( XM_019356832.2 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_019212377.1 Oreochromis niloticus phosphorylase b kinase regulatory subunit alpha, liver isoform (LOC100690562), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOr28625
Clone ID Related Accession (Same CDS sequence)	XM_019356832.1 , XM_019356832.2
Accession Version	XM_019356832.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031965.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_019356832.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACAG TGTCCTGGCG GTGTGGGGGC TGGGTATGGC CTACCGGAAG AATGCAGACC 
GCGATGAAGA CAAGGCCAAA GCTTACGAAC TGGAGCAGGT GTGTTTGCAA CATAAATAGA 
AGTGTCGTGA AACTGATGCA GGGGCTTCTG CAGTGCATGA TGAGACAGGT GGCCAAGGTG 
GAGAAGTTCA AACACACCCA GAGTACAAAG GATTGCTTGC ATGCCAAATA TGATACTCCC 
ACTTGTGCCA CAGTGGTCAG GGATGATCAG TGGGGTCATC TCCAGGTGGA CGCCACCTCG 
ATTTACCTGC TGATGCTGGC ACAGATGACA GCCTCAGGTC TTCGTATCAT TTCCAACCTG 
GACGAGGTAG CCTTCATCCA AAACTTGGTC TTCTACATAG AGGCTGCCTA CAAAGTAGCG 
GACTATGGAA TGTGGGAACG AGGTGACAAG ACTAACCAGG GCATTCCTGA GCTCAATGCC 
AGCTCTGTAG GAATGGCTAA GGCAGCGCTT GAGGCTATAG ATGAGCTGGA TCTGTTTGGT 
GCTCACGGAG GGCCGAAGTC AGTCATCCAT GTGTTGCCTG ATGAAGTCGA ACACTGTCAG 
TCTATTCTGT GCTCCATGCT GCCAAGAGCT TCAACTTCAA AGGAAATAGA TGCTGGTCTT 
CTGTCCATCA CTTCTTTCCC TGCGTTTGCT GTGGAGGATG CTGACCTGGT GGCTGTGACG 
AAGTCAGAGA TCATAAGCAA ACTGCAGGGT CGCTATGGCT GCTGTCGCTT TATCAGGGAC 
GGATATCGTT GTCCCAAAGA GGATCCATCT CGGCTGCATT ATGATCCTGC TGAGCTGAAG 
CTATTTGAGA ATATCGAGTG TGAGTGGCCT GTGTTTTGGA CTTACCTCAT CCTGGATGGT 
ATTTTTGCTG GTGATCAAGT TCAGGTGCAA GAGTACCGTG AAGCACTGGA GGGTGTTTTA 
ATCAGAGGGA AGAATGGCAT TAAGTTGGTG CCTGAACTTT ATTCTGTTCC TCCTGACAAG 
GTTGAGGAGG AGTATAGCAA TCCTCACTCA GTAGACAGGG TAGCCATGGG CCAGCTGCCA 
CACATGTGGG GACAGTCACT CTACATCTTG GGGTCCCTTG TGGCTGAGGG TTTTTTAGCA 
CCAGGAGAGA TAGATCCGCT CAACAGGAGA TTCTCTACAA GCTTCAAACC AGATGTGGTG 
GTACAAGTTT GTGTTCTAGC AGAGTCGGAG GAGATCCAGG AGTTGTTAAG AGATCTGGGG 
ATTGAGGTAC AGACGATGGC AGAAGTTCTG CCCATCAGGG TCATGCCAGC TCGCATCCTG 
AGCCATGTCT ATGTTAGACT GGGGAACAGC AAGAAGCTGA ATCTGAGTGG GCGGCCATAC 
AGACACATTG GTGTCCTGGG AACATCCAAA TTCTACGAGA TCCGAAATCG CTCTTACATA 
TTCACCCCTC AGTTTCTGGA TCAGCACCAT TTCTATCTGG CTCTGGACAA CCAGATGATT 
GTGGAGATGT TACGGACCGA GCTGGCCTAT CTCTCTTGTT GCTGGAGGAT GACAGGACGT 
CCCACGCTCA CATTCCCAAT CACCCGTAGC ATGCTGGATG AGGATGGAGA AACAATTGAT 
TCATGTATCC TATCAACTCT AAGGAAACTG CAGGATGGCT ATTTTGCTGG AGCGAGGGTG 
CAGATGTCAG ACGTCTCCAG TGTCCAGACC ACTTCGTTTC ACACTCGTCT CAGCTTCCTG 
GATGAAGAGA ATGATGACAT ATTACTTGAG GATGAAGGCG ATGATGATGA TGGATATGGA 
GAGGAGTATA ATACCTGTGG TCCCTCGGAG GACTCAAAAG ATGTATTTGA CCAGTACCTC 
ACCCAGCTCC TTCACAGCAC CACTACCAAG TGCCATCTTC CTCCCATCCA GAGGGGGCAG 
CACCACGTCT TCAGTCCAGA ACATACCACA AGAGACATCC TGTCTTTTAT GGCGCAGGTC 
CAGGGCCTGA ACGTTCCCAA GTCTTCCATG TATCTGCCTG TGACTCCGGT CAAGAGCAAA 
CATCGCAAAT CTCTCAACCT TCTTGATGTT CCTCATCCTC AGCACGGCCC ACAGGCGAAA 
CAGCACAAGC CACACTCTGC TGCTGATCTT CACCTGCCTC GAGACTCTCA GGGCAACACA 
GACTTTGCAG CGTTGGTCAG GCAGCTAAAA GAATGCCCCA CTCTTCAGGA CCAGGCTGAC 
ATCCTCTACA TTCTCTATGT GATGAAAGGA GCTGATTGGC TGGTGGAGTT GTCAGGTCCT 
GGGCAGGGTG GGGTCAGCGT GCGATCACTG TTGGAGGAGC TGTACGTAGA AGCCGGAGCC 
TGCAAAGAGT GGGCGCTCAT TAGGTACATC TCTGGAATAC TGCGCAAGAG GGTGGAGGTC 
CTCGCTGAGG CCTGCACAGA TCTGATTTCC CATCACAAGC AGCTGACTGT AGGTCTACCT 
CCTCAACCCA GGGAAAGAGT GATCACAGTC CCTCTTCCTC CGGATGAGTT AAACAGCCTC 
ATCTACGAAG CCAGTGGTCA GGACATAAGT GTAGCAGTGC TCACTCAGGA AATTATGGTG 
TATCTAGCCA TGTATGTGCG CTCCCAGCCC GCTCTGTTCG GGGACATGCT CAGACTCAGG 
ATAGGACTCA TCATGCAAGT GATGGCCACT GAGTTAGCTC GTAGCCTGCA CTGTTCTGGG 
GAGGAGGCGT CAGAGAGTTT GATGAGCCTG AGTCCATTTG GCATGAAGAA CCTACTGCAT 
CACATCCTCA GTGGCAAAGA ATTTGGGGTG GAGAGAAGCA TGCGCCCAAT CCAGTCTACA 
GCCACTAGCC CTGCGATATC CATCCATGAA CTAGGTCACA CGGGAGCTAC AAAGACTGAA 
CGCACAGGAA TACACAAGCT AAAGAGTGAG ATAAAACAGC TGGATGACTC TCGCCCTACA 
AGTATCTTCA GTGGCGGTTT TTCCTTGAGT AGCAATGTCA CATCTCCTCG CTCTACGCGT 
TGCAGCAGCC CTTCCACTCC CAGTGGAATG CTGTCCCCGG TGAGCACTTG TCCAGGAGAC 
GGTCAGCTGC ACTGGGAGGA GAGGCAAGGT CAGTGGCTGA GGAGACGCAG GCTAGATGGT 
GCCATCAACA GAGTACCTAT GGGTTTCTAC CAAAAGGTGT GGAAGATCCT GCAGAAGTGC 
CACGGTCTGT CCATCGACGG ATACGTGTTG CCCTCTTCTA CCACAAGAGA GATGACAGCA 
GGAGAGATTA AGTTTGCAGT GCAGGTTGAA TCTGTGCTGA ACCACGTCCC ACAGCCAGAG 
TACCGGCAGC TGCTAGTGGA GACTGTGATG GTTGTGGGCC TGATAGCGGA CGTAGACGTG 
GACAACATCG GCGGCATCAT CCACGTGGAT CGCGTGTTGC ATTTGGCCAA TGACCTTTTC 
CTCAGTGACC AGAAATCCCA CGGCGCCAGT GAGTATTTCC TTGAGAAGGA CCCAGCAACC 
GGAATCTGCA ACTTTTTCTA CGACAGCGCT CCTAGTGGTA GCTATGGCAC CATGACCTAT 
CTCTCCAAAG CGGCAGTCAC TTACGTCCAG GACTTCCTGC CAAGTTCCAG CTGCCTGATG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019212377.1
CDS	17..3622
Translation	MSTVSWRCGGWVWPTGRMQTAMKTRPKLTNWSRCVCNINRSVVKLMQGLLQCMMRQVAKVEKFKHTQSTKDCLHAKYDTPTCATVVRDDQWGHLQVDATSIYLLMLAQMTASGLRIISNLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLFGAHGGPKSVIHVLPDEVEHCQSILCSMLPRASTSKEIDAGLLSITSFPAFAVEDADLVAVTKSEIISKLQGRYGCCRFIRDGYRCPKEDPSRLHYDPAELKLFENIECEWPVFWTYLILDGIFAGDQVQVQEYREALEGVLIRGKNGIKLVPELYSVPPDKVEEEYSNPHSVDRVAMGQLPHMWGQSLYILGSLVAEGFLAPGEIDPLNRRFSTSFKPDVVVQVCVLAESEEIQELLRDLGIEVQTMAEVLPIRVMPARILSHVYVRLGNSKKLNLSGRPYRHIGVLGTSKFYEIRNRSYIFTPQFLDQHHFYLALDNQMIVEMLRTELAYLSCCWRMTGRPTLTFPITRSMLDEDGETIDSCILSTLRKLQDGYFAGARVQMSDVSSVQTTSFHTRLSFLDEENDDILLEDEGDDDDGYGEEYNTCGPSEDSKDVFDQYLTQLLHSTTTKCHLPPIQRGQHHVFSPEHTTRDILSFMAQVQGLNVPKSSMYLPVTPVKSKHRKSLNLLDVPHPQHGPQAKQHKPHSAADLHLPRDSQGNTDFAALVRQLKECPTLQDQADILYILYVMKGADWLVELSGPGQGGVSVRSLLEELYVEAGACKEWALIRYISGILRKRVEVLAEACTDLISHHKQLTVGLPPQPRERVITVPLPPDELNSLIYEASGQDISVAVLTQEIMVYLAMYVRSQPALFGDMLRLRIGLIMQVMATELARSLHCSGEEASESLMSLSPFGMKNLLHHILSGKEFGVERSMRPIQSTATSPAISIHELGHTGATKTERTGIHKLKSEIKQLDDSRPTSIFSGGFSLSSNVTSPRSTRCSSPSTPSGMLSPVSTCPGDGQLHWEERQGQWLRRRRLDGAINRVPMGFYQKVWKILQKCHGLSIDGYVLPSSTTREMTAGEIKFAVQVESVLNHVPQPEYRQLLVETVMVVGLIADVDVDNIGGIIHVDRVLHLANDLFLSDQKSHGASEYFLEKDPATGICNFFYDSAPSGSYGTMTYLSKAAVTYVQDFLPSSSCLMQ

Target ORF information:

RefSeq Version	XM_019356832.2
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus phosphorylase b kinase regulatory subunit alpha, liver isoform (LOC100690562), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019356832.2

ORF Insert Sequence:

ATGTCTACAG TGTCCTGGCG GTGTGGGGGC TGGGTATGGC CTACCGGAAG AATGCAGACC 
GCGATGAAGA CAAGGCCAAA GCTTACGAAC TGGAGCAGGT GTGTTTGCAA CATAAATAGA 
AGTGTCGTGA AACTGATGCA GGGGCTTCTG CAGTGCATGA TGAGACAGGT GGCCAAGGTG 
GAGAAGTTCA AACACACCCA GAGTACAAAG GATTGCTTGC ATGCCAAATA TGATACTCCC 
ACTTGTGCCA CAGTGGTCAG GGATGATCAG TGGGGTCATC TCCAGGTGGA CGCCACCTCG 
ATTTACCTGC TGATGCTGGC ACAGATGACA GCCTCAGGTC TTCGTATCAT TTCCAACCTG 
GACGAGGTAG CCTTCATCCA AAACTTGGTC TTCTACATAG AGGCTGCCTA CAAAGTAGCG 
GACTATGGAA TGTGGGAACG AGGTGACAAG ACTAACCAGG GCATTCCTGA GCTCAATGCC 
AGCTCTGTAG GAATGGCTAA GGCAGCGCTT GAGGCTATAG ATGAGCTGGA TCTGTTTGGT 
GCTCACGGAG GGCCGAAGTC AGTCATCCAT GTGTTGCCTG ATGAAGTCGA ACACTGTCAG 
TCTATTCTGT GCTCCATGCT GCCAAGAGCT TCAACTTCAA AGGAAATAGA TGCTGGTCTT 
CTGTCCATCA CTTCTTTCCC TGCGTTTGCT GTGGAGGATG CTGACCTGGT GGCTGTGACG 
AAGTCAGAGA TCATAAGCAA ACTGCAGGGT CGCTATGGCT GCTGTCGCTT TATCAGGGAC 
GGATATCGTT GTCCCAAAGA GGATCCATCT CGGCTGCATT ATGATCCTGC TGAGCTGAAG 
CTATTTGAGA ATATCGAGTG TGAGTGGCCT GTGTTTTGGA CTTACCTCAT CCTGGATGGT 
ATTTTTGCTG GTGATCAAGT TCAGGTGCAA GAGTACCGTG AAGCACTGGA GGGTGTTTTA 
ATCAGAGGGA AGAATGGCAT TAAGTTGGTG CCTGAACTTT ATTCTGTTCC TCCTGACAAG 
GTTGAGGAGG AGTATAGCAA TCCTCACTCA GTAGACAGGG TAGCCATGGG CCAGCTGCCA 
CACATGTGGG GACAGTCACT CTACATCTTG GGGTCCCTTG TGGCTGAGGG TTTTTTAGCA 
CCAGGAGAGA TAGATCCGCT CAACAGGAGA TTCTCTACAA GCTTCAAACC AGATGTGGTG 
GTACAAGTTT GTGTTCTAGC AGAGTCGGAG GAGATCCAGG AGTTGTTAAG AGATCTGGGG 
ATTGAGGTAC AGACGATGGC AGAAGTTCTG CCCATCAGGG TCATGCCAGC TCGCATCCTG 
AGCCATGTCT ATGTTAGACT GGGGAACAGC AAGAAGCTGA ATCTGAGTGG GCGGCCATAC 
AGACACATTG GTGTCCTGGG AACATCCAAA TTCTACGAGA TCCGAAATCG CTCTTACATA 
TTCACCCCTC AGTTTCTGGA TCAGCACCAT TTCTATCTGG CTCTGGACAA CCAGATGATT 
GTGGAGATGT TACGGACCGA GCTGGCCTAT CTCTCTTGTT GCTGGAGGAT GACAGGACGT 
CCCACGCTCA CATTCCCAAT CACCCGTAGC ATGCTGGATG AGGATGGAGA AACAATTGAT 
TCATGTATCC TATCAACTCT AAGGAAACTG CAGGATGGCT ATTTTGCTGG AGCGAGGGTG 
CAGATGTCAG ACGTCTCCAG TGTCCAGACC ACTTCGTTTC ACACTCGTCT CAGCTTCCTG 
GATGAAGAGA ATGATGACAT ATTACTTGAG GATGAAGGCG ATGATGATGA TGGATATGGA 
GAGGAGTATA ATACCTGTGG TCCCTCGGAG GACTCAAAAG ATGTATTTGA CCAGTACCTC 
ACCCAGCTCC TTCACAGCAC CACTACCAAG TGCCATCTTC CTCCCATCCA GAGGGGGCAG 
CACCACGTCT TCAGTCCAGA ACATACCACA AGAGACATCC TGTCTTTTAT GGCGCAGGTC 
CAGGGCCTGA ACGTTCCCAA GTCTTCCATG TATCTGCCTG TGACTCCGGT CAAGAGCAAA 
CATCGCAAAT CTCTCAACCT TCTTGATGTT CCTCATCCTC AGCACGGCCC ACAGGCGAAA 
CAGCACAAGC CACACTCTGC TGCTGATCTT CACCTGCCTC GAGACTCTCA GGGCAACACA 
GACTTTGCAG CGTTGGTCAG GCAGCTAAAA GAATGCCCCA CTCTTCAGGA CCAGGCTGAC 
ATCCTCTACA TTCTCTATGT GATGAAAGGA GCTGATTGGC TGGTGGAGTT GTCAGGTCCT 
GGGCAGGGTG GGGTCAGCGT GCGATCACTG TTGGAGGAGC TGTACGTAGA AGCCGGAGCC 
TGCAAAGAGT GGGCGCTCAT TAGGTACATC TCTGGAATAC TGCGCAAGAG GGTGGAGGTC 
CTCGCTGAGG CCTGCACAGA TCTGATTTCC CATCACAAGC AGCTGACTGT AGGTCTACCT 
CCTCAACCCA GGGAAAGAGT GATCACAGTC CCTCTTCCTC CGGATGAGTT AAACAGCCTC 
ATCTACGAAG CCAGTGGTCA GGACATAAGT GTAGCAGTGC TCACTCAGGA AATTATGGTG 
TATCTAGCCA TGTATGTGCG CTCCCAGCCC GCTCTGTTCG GGGACATGCT CAGACTCAGG 
ATAGGACTCA TCATGCAAGT GATGGCCACT GAGTTAGCTC GTAGCCTGCA CTGTTCTGGG 
GAGGAGGCGT CAGAGAGTTT GATGAGCCTG AGTCCATTTG GCATGAAGAA CCTACTGCAT 
CACATCCTCA GTGGCAAAGA ATTTGGGGTG GAGAGAAGCA TGCGCCCAAT CCAGTCTACA 
GCCACTAGCC CTGCGATATC CATCCATGAA CTAGGTCACA CGGGAGCTAC AAAGACTGAA 
CGCACAGGAA TACACAAGCT AAAGAGTGAG ATAAAACAGC TGGATGACTC TCGCCCTACA 
AGTATCTTCA GTGGCGGTTT TTCCTTGAGT AGCAATGTCA CATCTCCTCG CTCTACGCGT 
TGCAGCAGCC CTTCCACTCC CAGTGGAATG CTGTCCCCGG TGAGCACTTG TCCAGGAGAC 
GGTCAGCTGC ACTGGGAGGA GAGGCAAGGT CAGTGGCTGA GGAGACGCAG GCTAGATGGT 
GCCATCAACA GAGTACCTAT GGGTTTCTAC CAAAAGGTGT GGAAGATCCT GCAGAAGTGC 
CACGGTCTGT CCATCGACGG ATACGTGTTG CCCTCTTCTA CCACAAGAGA GATGACAGCA 
GGAGAGATTA AGTTTGCAGT GCAGGTTGAA TCTGTGCTGA ACCACGTCCC ACAGCCAGAG 
TACCGGCAGC TGCTAGTGGA GACTGTGATG GTTGTGGGCC TGATAGCGGA CGTAGACGTG 
GACAACATCG GCGGCATCAT CCACGTGGAT CGCGTGTTGC ATTTGGCCAA TGACCTTTTC 
CTCAGTGACC AGAAATCCCA CGGCGCCAGT GAGTATTTCC TTGAGAAGGA CCCAGCAACC 
GGAATCTGCA ACTTTTTCTA CGACAGCGCT CCTAGTGGTA GCTATGGCAC CATGACCTAT 
CTCTCCAAAG CGGCAGTCAC TTACGTCCAG GACTTCCTGC CAAGTTCCAG CTGCCTGATG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

LOC100690562 cDNA ORF clone, Oreochromis niloticus(Nile tilapia)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム